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Expression of monellin in a food-grade delivery system in Saccharomyces cerevisiae

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成果类型:
期刊论文
作者:
Liu, Jun;Yan, Da-zhong*;Zhao, Sheng-jun
通讯作者:
Yan, Da-zhong
作者机构:
[Yan, Da-zhong; Liu, Jun] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
[Zhao, Sheng-jun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.
通讯机构:
[Yan, Da-zhong] W
Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
语种:
英文
关键词:
fungal DNA;ribosome DNA;sweetening agent;vegetable protein;Africa;chemistry;food handling;fruit;gene expression;genetic transformation;genetics;human;Menispermaceae;metabolism;plant gene;Saccharomyces cerevisiae;Africa, Western;DNA, Fungal;DNA, Ribosomal;Food Technology;Fruit;Gene Expression;Genes, Plant;Humans;Menispermaceae;Plant Proteins;Saccharomyces cerevisiae;Sweetening Agents;Transformation, Genetic
期刊:
Journal of the Science of Food and Agriculture
ISSN:
0022-5142
年:
2015
卷:
95
期:
13
页码:
2646-2651
基金类别:
Natural Science Foundation of Hubei Province of ChinaNatural Science Foundation of Hubei Province [2008CDB067]
机构署名:
本校为第一且通讯机构
院系归属:
生命科学与技术学院
动物科学与营养工程学院
摘要:
BACKGROUND: Genetically modified (GM) foods have caused much controversy. Construction of a food-grade delivery system is a desirable technique with presumptive impact on industrial applications from the perspective of bio-safety. The aim of this study was to construct a food-grade delivery system for Saccharomyces cerevisiae and to study the expression of monellin from the berries of the West African forest plant Dioscoreophyllum cumminsii in this system. RESULTS: A food-grade system for S. cerevisiae was constructed based on ribosomal DNA (rD...

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