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Influences of epigallocatechin gallate and citric acid on Escherichia coli O157:H7 toxin gene expression and virulence‐associated stress response

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成果类型:
期刊论文
作者:
Yang, J.;Tang, C. B.;Xiao, J.;Du, W. F.;Li, R.*
通讯作者:
Li, R.
作者机构:
[Li, R.; Yang, J.; Xiao, J.; Du, W. F.] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.
[Tang, C. B.] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing, Jiangsu, Peoples R China.
通讯机构:
[Li, R.] W
Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.
语种:
英文
关键词:
citric acid;Escherichia coli O157:H7;EGCG;membrane permeability;toxin expression
期刊:
Letters in Applied Microbiology
ISSN:
0266-8254
年:
2018
卷:
67
期:
5
页码:
435-441
基金类别:
This work was funded by the National Key R&D Program of China (Grant No.2017YFC1600103); partially by a grant of National Natural Science Foundation of China (31471660).
机构署名:
本校为第一且通讯机构
院系归属:
生命科学与技术学院
摘要:
Citric acid and EGCG at their minimum inhibitory concentrations were tested in this study. Logarithmic phase cells of Escherichia coli O157:H7 (ATCC 43895) were exposed to EGCG and citric acid respectively. The results of RT-real time PCR showed that both EGCG and citric acid increased stx2 and oxyR expression and decreased stx1, recA and Q expression. The result of Western blotting for RecA protein further indicated that both EGCG and citric acid decreased RecA production. Both EGCG and citric acid increased the level of intracellular reactive...

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