期刊论文

Chen, Shouwen

英文

Bioresource Technology

0960-8524

2019

271

325-331

10.1016/j.biortech.2018.08.134

The total RNA was extracted by according to the previous report (Li et al., 2012b). Before RNA extraction, all the samples were filtrated through filter paper to remove bean dreg residue. The HiScript®II Q RT SuperMix for qPCR (+gDNA wiper) (Vazyme, China) was used for DNA digestion and cDNA synthesis following the manufacturer’s instructions. The real-time PCR was performed by using iTaq™ Universal SYBR® Green Supermix (BIO-RAD, USA) by the manufacturer. The 16S rRNA gene was served as the

本校为其他机构

Short branched-chain fatty acids (SBCFAs) are multi-functional platform chemicals used in many fields. Currently, SBCFAs are produced mainly by chemical synthesis, which is high cost and lead to environmental pollution. The aim of this study was to achieve high-level production of SBCFAs from waste materials, bean dreg and crude glycerol. The Bacillus licheniformis DWc9n∗ was genetically modified by overexpression of SBCFAs synthesis genes via replacement of native promoter of bkd operon, the mutant strain DWc9n∗-PbacA produced 4.68 g/L of SB...