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De Novo Biosynthesis of Polydatin in Saccharomyces cerevisiae

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成果类型:
期刊论文
作者:
Liu, Tian;Liu, Yuqian;Li, Lan;Liu, Xiaonan;Guo, Zhaokuan;...
通讯作者:
Xiaonan Liu<&wdkj&>Nengzhong Xie<&wdkj&>Jian Lu<&wdkj&>Huifeng Jiang
作者机构:
[Lu, Jian; Liu, Tian] Guangxi Univ, Life Sci & Technol Coll, Nanning 530004, Guangxi, Peoples R China.
[Lu, Lina; Liu, Yuqian; Jiang, Huifeng; Liu, Tian; Guo, Zhaokuan; Liu, Xiaonan; Zhu, Xiaoxi; Cheng, Jian] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China.
[Lu, Lina; Liu, Yuqian; Jiang, Huifeng; Liu, Tian; Guo, Zhaokuan; Liu, Xiaonan; Cheng, Jian] Natl Ctr Technol Innovat Synthet Biol, Tianjin 300308, Peoples R China.
[Liu, Yuqian] South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China.
[Fan, Guanwei; Li, Lan] Tianjin Univ Tradit Chinese Med, Teaching Hosp 1, Tianjin 300193, Peoples R China.
通讯机构:
[Xiaonan Liu; Huifeng Jiang] K
[Nengzhong Xie] N
[Jian Lu] L
Life Science and Technology College, Guangxi University, Nanning, Guangxi 530004, China<&wdkj&>Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China<&wdkj&>National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China<&wdkj&>Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China<&wdkj&>University of Chinese Academy of Sciences, Beijing 100049, China<&wdkj&>National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China<&wdkj&>National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China
语种:
英文
关键词:
Polygonum cuspidatum;polydatin;resveratrol;glycosyltransferase;synthetic biology
期刊:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN:
0021-8561
年:
2021
卷:
69
期:
21
页码:
5917-5925
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [32060523, 31901026, 21868007]; Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project [TSBICIP-KJGG-007]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2019M661032]
机构署名:
本校为其他机构
院系归属:
生命科学与技术学院
摘要:
Polydatin, with better structural stability and biological activities than resveratrol, is mainly extracted from the traditional Chinese medicinal plant Polygonum cuspidatum. In this study, based on the transcriptome analysis of P. cuspidatum, we identified the key glycosyltransferase of resveratrol and achieved the biosynthesis of polydatin from glucose by incorporation with the resveratrol biosynthesis module, UDP-glucose supply module, and glycosyltransferase expression module. Through metabolic engineering and fermentation optimization, the production of polydatin reached 545 mg/L, and the...

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