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MOLECULAR CLONING AND EXPRESSION ANALYSIS OF A PUTATIVE E CLASS MADS-BOX GENE, GbSEP, FROM GINKGO BILOBA

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成果类型:
期刊论文
作者:
Cheng, Shui-Yuan;Cheng, Jun-Huan;Xu, Feng*;Ye, Jia-Bao;Wang, Xiao-Hui
通讯作者:
Xu, Feng
作者机构:
[Cheng, Shui-Yuan] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
[Cheng, Jun-Huan; Xu, Feng; Wang, Xiao-Hui; Ye, Jia-Bao] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Peoples R China.
通讯机构:
[Xu, Feng] Y
Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Peoples R China.
语种:
英文
关键词:
Expression analysis;GbSEP;Ginkgo biloba;MADS-box;Real-time PCR
期刊:
JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS
ISSN:
1018-7081
年:
2016
卷:
26
期:
1
页码:
253-260
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370680]; Natural Science Foundation of Hubei ProvinceNatural Science Foundation of Hubei Province [2013CFA039]; Key Projects of Chinese Ministry of Education [212112, 210137]
机构署名:
本校为第一机构
院系归属:
生命科学与技术学院
摘要:
MADS-box gene family plays an important role in the development of floral organs in plants. A full-length cDNA of MADS-box gene, named as GbSEP(Genbank accession numberKJ630504), was cloned by RT-PCR from female flower of Ginkgo biloba. The cDNA sequence of GbSEP contained an ORF of 756 bp encoding 252 amino acid residues. The comparison between cDNA and genomic sequences of GbSEP indicated no intron in the genomic DNA sequence of this gene. Protein multi-alignment analysis indicated that GbSEP shared high homology with other SEP family protein...

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