Cloning, Expression Profiling and Functional Analysis of CnHMGS, a Gene Encoding 3-hydroxy-3-Methylglutaryl Coenzyme A Synthase from Chamaemelum nobile

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成果类型：

期刊论文

作者：

Cheng, Shuiyuan;Wang, Xiaohui;Xu, Feng*;Chen, Qiangwen;Tao, Tingting;...

通讯作者：

Xu, Feng;Chang, Jie

作者机构：

[Cheng, Shuiyuan] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

[Chen, Qiangwen; Xu, Feng; Wang, Xiaohui; Zhang, Weiwei; Lei, Jing; Tao, Tingting; Liao, Yongling] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.

[Chang, Jie] Jingchu Univ Technol, Hubei Collaborat Innovat Ctr Targeted Antitumor D, Jingmen 448000, Hubei, Peoples R China.

[Li, Xingxiang] Yangtze Univ, Sch Med, Jingzhou 434025, Hubei, Peoples R China.

通讯机构：

[Xu, Feng] Y

[Chang, Jie] J

Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.

Jingchu Univ Technol, Hubei Collaborat Innovat Ctr Targeted Antitumor D, Jingmen 448000, Hubei, Peoples R China.

语种：

英文

关键词：

Chamaemelum nobile;sesquiterpenoid biosynthesis;3-hydroxy-3-methylglutaryl coenzyme A synthase;cloning;expression profile;functional complementation analysis

期刊：

Molecules

ISSN：

1420-3049

年：

2016

卷：

期：

页码：

316

DOI：

10.3390/molecules21030316

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31400603]; International Science and Technology Cooperation Project of Hubei Province [2013BHE029, 2013BHE039]

机构署名：

本校为第一机构

院系归属：

生命科学与技术学院

摘要：

Roman chamomile (Chamaemelum nobile L.) is renowned for its production of essential oils, which major components are sesquiterpenoids. As the important enzyme in the sesquiterpenoid biosynthesis pathway, 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) catalyze the crucial step in the mevalonate pathway in plants. To isolate and identify the functional genes involved in the sesquiterpene biosynthesis of C. nobile L., a HMGS gene designated as CnHMGS (GenBank Accession No. KU529969) was cloned from C. nobile. The cDNA sequence of CnHMGS contained a 1377 bp open reading frame encoding a 458...

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Cloning, Expression Profiling and Functional Analysis of CnHMGS, a Gene Encoding 3-hydroxy-3-Methylglutaryl Coenzyme A Synthase from Chamaemelum nobile

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