期刊论文

Yang, S.;Shen, W.

英文

Fermentation

2311-5637

2023

9

2

113-

10.3390/fermentation9020113

Supported by the National Natural Science Foundation of China (U1932141 and 21978071), 2022 Joint Projects between Chinese and CEEC‘s Universities (202004), the National Key Technology Research and Development Program of China (2022YFA0911800 and 2018YFA0900300), the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang Province (2018R01014), and the Innovation Base for Introducing Talents of Discipline of Hubei Province (2019BJH021). Funding was also supported by the State Key Laboratory of Biocatalysis and Enzyme Engineering.

本校为其他机构

Biorefinery to produce value-added biochemicals offers a promising alternative to meet our sustainable energy and environmental goals. Acetoin is widely used in the food and cosmetic industries as taste and fragrance enhancer. The generally regarded as safe (GRAS) bacterium Zymomonas mobilis produces acetoin as an extracellular product under aerobic conditions. In this study, metabolic engineering strategies were applied including redistributing the carbon flux to acetoin and manipulating the NADH levels. To improve the acetoin level, a heterologous acetoin pathway was first introduced into Z....