期刊论文

Hou, Yongqing

英文

Amino Acids

0939-4451

2015

47

1

65-78

10.1007/s00726-014-1842-8

This research was jointly supported by National Basic Research Program of China (No. 2012CB126305), National Natural Science Foundation of China (No. 31372319 and 31402084), Hubei Provincial Research and Development Program (No. 2010BB023), Natural Science Foundation of Hubei Province (No. 2013CFA097, 2013CFB325, 2012FFB04805, 2011CDA131), the Hubei Hundred Talent program, Agriculture and Food Research Initiative Competitive Grant (2014-67015-21770) of the USDA National Institute of Food and Agriculture, and Texas AgriLife Research (H-82000). All these funding agencies had no role in the design, analysis or writing of this study.

本校为第一且通讯机构

Neonates (including human infants) require l-glutamine (Gln) for optimal intestinal health. This study tested the hypothesis that Gln enhances enterocyte growth via both mammalian target of rapamycin (mTOR) and AMP-activated kinase (AMPK) signaling pathways. Intestinal porcine epithelial cells (IPEC-1) were cultured for 3 days in Gln-free Dulbecco's modified Eagle medium containing 0 or 2 mM Gln. To determine the role of mTOR and AMPK on cell growth, additional experiments were conducted where medium contained 2 mM Gln and 10 nM rapamycin (Rap,...