Multi-Fluorescence Real-Time PCR Assay for Detection of RIF and INH Resistance of M-tuberculosis

首页 > 成果 > 详情

认领

导出

Link by DOI

反馈

作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Peng, Jingfu;Yu, Xiaoli;Cui, Zhenling;Xue, Wenfei;Luo, Ziyi;...

通讯作者：

Li, Yao;Zhang, Shulin

作者机构：

[Li, Yao; Xue, Wenfei; Jiang, Danqing; Peng, Jingfu; Liu, Minghua; Wu, Hai] Fudan Univ, Sch Life Sci, Shanghai Engn Res Ctr Ind Microorgan, State Key Lab Genet Engn, Shanghai 200433, Peoples R China.

[Wen, Zilu; Yu, Xiaoli] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Dept Biotechnol, Wuhan, Peoples R China.

[Cui, Zhenling] Tongji Univ, Shanghai Pulm Hosp, Sch Med, Shanghai Key Lab TB, Shanghai 200092, Peoples R China.

[Luo, Ziyi] Third Peoples Hosp Shenzhen, Shenzhen, Peoples R China.

[Zheng, Heping] Haoding Technol Ltd Co, Shenzhen, Peoples R China.

通讯机构：

[Li, Yao] F

[Zhang, Shulin] S

Fudan Univ, Sch Life Sci, Shanghai Engn Res Ctr Ind Microorgan, State Key Lab Genet Engn, Shanghai 200433, Peoples R China.

Shanghai Jiao Tong Univ, Sch Med, Dept Immunol & Med Microbiol, Shanghai 200030, Peoples R China.

语种：

英文

关键词：

Mycobacterium tuberculosis;drug resistance;isoniazid;quantitative real-time PCR;rifampicin

期刊：

Frontiers in Microbiology

ISSN：

1664-302X

年：

2016

卷：

期：

APR

页码：

618

DOI：

10.3389/fmicb.2016.00618

基金类别：

National Key Program of China [2013ZX10003003-004-002]; Shanghai Science and Technology CommissionShanghai Science & Technology CommitteeScience & Technology Commission of Shanghai Municipality (STCSM) [13DZ2252000]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81271794]; Educational Commission of Hubei china [D20121809]

机构署名：

本校为其他机构

院系归属：

生命科学与技术学院

摘要：

Background: Failure to early detect multidrug-resistant tuberculosis (MDR-TB) results in treatment failure and poor clinical outcomes, and highlights the need to rapidly detect resistance to rifampicin (RIF) and isoniazid (INH). Methods: In Multi-Fluorescence quantitative Real-Time PCR (MF-qRT-PCR) assay, 10 probes labeled with four kinds of fluorophores were designed to detect the mutations in regions of rpoB, katG, mabA-inhA, oxyR-ahpC, and rrs. The efficiency of MF-qRT-PCR assay was tested using 261 bacterial isolates and 33 clinical sputum ...

反馈

产权有误：本人成果被他人认领

数据有误：数据基本信息有误

归属有误：成果的院系归属、机构署名归属有误

其他原因：

验证码：

看不清楚，换一个

确定

取消

成果认领

标题：

用户	作者	通讯作者	--
	请选择	请选择	--

确定

取消

Multi-Fluorescence Real-Time PCR Assay for Detection of RIF and INH Resistance of M-tuberculosis

反馈

成果认领

提示

该栏目需要登录且有访问权限才可以访问