期刊论文

Hou, Yongqing

英文

FRONTIERS IN BIOSCIENCE-LANDMARK

2768-6701

2018

23

8

1517-1534

10.2741/4658

This research was jointly supported by National Key R & D Programs of China (2017YFD0500505, 2016YFD0501210), National Natural Science Foundation of China (31372319, 31572416, 31402084), Hubei Provincial Technology and Innovation Program (2016ABA121, 2017AHB062), Natural Science Foundation of Hubei Province (2016CFA070), Research and Innovation Initiatives of WHPU (2016J08), the Hubei Hundred Talent Program, Agriculture and Food Research Initiative Competitive Grants of (2014-67015-21770) of the USDA National Institute of Food and Agriculture, and Texas AgriLife Research (H-82000). All these funding agencies had no role in the design, analysis or writing of our published studies. The authors declare that they have no conflict of interest.

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Enterotoxigenic Escherichia coli strains induce human and animal intestinal dysfunction and injury, and cause diarrhea in weanling pigs. A recombinant E.coli strain LMG194-STa which expressed single toxin STa of ETEC was constructed by directly inserting the STa gene of ETEC into the expression vector pBAD202 and then transferring the recombinant plasmid pBAD-STa into the E.coli LMG19 host strain. Diarrhea and intestinal injury in piglets were induced by oral administration of recombinant strain LMG194-Sta. In vitro, the recombinant strain LMG1...