期刊论文

中文

食品与发酵工业

0253-990X

2015

41

11

87-92

10.13995/j.cnki.11-1802/ts.201511016

国家公益性科技支撑计划项目:粮油加工技术与装备开发应用（201313012-03）；

本校为第一机构

分别研究了柠檬酸添加量、酶加入量、温度、加水量和时间5个单因素对磷脂酶C(PLC)脱胶效果的影响,在单因素基础上,采用响应面试验设计,对主要脱胶工艺参数进行优化,得到最佳条件:柠檬酸添加量5.80 mL/kg,加酶量12.60 mL/kg,酶解温度42.20 ℃,在该条件下验证,菜籽毛油磷含量由693 mg/kg降低至7.85 mg/kg。采用棒状薄层色谱-氢火焰离子化检测器(TLC-FID)对毛油与脱胶油中甘油酯含量进行检测,结果表明:脱胶油中甘一酯的含量变化不明显,甘二酯含量从约2.5%增加到约6.5%, 而甘三酯的含量从约93%下降到约89%;而同样采用TLC-FID对水化脱胶磷脂与PLC脱胶磷脂中的溶血磷脂进行检测,分析得知,PLC脱胶磷脂中...

The influences of citric acid dosage, enzyme dosage, degumming temperature, amount of water addi- tion and degumming time on the phospholipids removal efficiency from rapeseed oil were investigated based on the sin- gle-factor experiments. Response surface methodology （RSM） was adopted to optimize the main processing parameters of PLC degumming, the optimal processing conditions were as follows： citric acid dosage 5.80 mL/kg, enzyme dosage 12.60 mL/kg, degumming temperature 42.20℃. Under the optimal conditions, the phosphorus content of de-...