期刊论文

Optimization and secretory expression of the alkaline protease derived from Alkalihalobacillus alcalophilus in Bacillus subtilis

中文

食品与发酵工业

Food and Fermentation Industries

0253-990X

2024

50

18

91-97

10.13995/j.cnki.11-1802/ts.037573

国家重点研发计划(2021YFC2100202)。

本校为第一机构

该研究通过信号肽筛选和启动子优化构建了一种在枯草芽孢杆菌中高效分泌嗜碱碱性卤杆菌蛋白酶的表达系统。结果表明,信号肽筛选和启动子优化的组合策略显著提高了该蛋白酶在枯草芽孢杆菌中的胞外表达量。在173种信号肽中,SP展开更多 该研究通过信号肽筛选和启动子优化构建了一种在枯草芽孢杆菌中高效分泌嗜碱碱性卤杆菌蛋白酶的表达系统。结果表明,信号肽筛选和启动子优化的组合策略显著提高了该蛋白酶在枯草芽孢杆菌中的胞外表达量。在173种信号肽中,SPYdjm介导该蛋白酶分泌的效率最高,是原始信号肽SPaprE所介导酶活力的13.8倍。而串联启动子PHapII-Pylb对表达的促进作用最为显著,使酶活力进一...

The protease produced by Alkalihalobacillus alcalophilus performed high catalytic activity in alkaline and halophilic environments,which can be widely used in leather and detergent industry.In this study,a highly efficient expression system was constructed in Bacillus s...MORE The protease produced by Alkalihalobacillus alcalophilus performed high catalytic activity in alkaline and halophilic environments,which can be widely used in leather and detergent industry.In this study,a highly efficient expression system was constructed in Bacillus subtilis through signal peptide screening and promote...