A Color-Reaction-Based Biochip Detection Assay for RIF and INH Resistance of Clinical Mycobacterial Specimens

首页 > 成果 > 详情

认领

导出

Link by DOI

反馈

作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Xue, Wenfei;Peng, Jingfu;Yu, Xiaoli;Zhang, Shulin;Zhou, Boping;...

通讯作者：

Li, Yao;Zhu, Bin

作者机构：

[Li, Yao; Xue, Wenfei; Jiang, Danqing; Peng, Jingfu] Fudan Univ, State Key Lab Genet Engn, Shanghai Engn Res Ctr Ind Microorganisms, Sch Life Sci, Shanghai 200433, Peoples R China.

[Yu, Xiaoli; Ding, Bingbing] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

[Zhang, Shulin] Shanghai Jiao Tong Univ, Sch Med, Dept Med Microbiol & Parasitol, Shanghai 200025, Peoples R China.

[Zhou, Boping; Chen, Jianbo] Third Peoples Hosp Shenzhen, Shenzhen 518000, Peoples R China.

[Zhu, Bin] Baio Technol Ltd Co, Shanghai 200233, Peoples R China.

通讯机构：

[Li, Yao] F

[Zhu, Bin] B

Fudan Univ, State Key Lab Genet Engn, Shanghai Engn Res Ctr Ind Microorganisms, Sch Life Sci, Shanghai 200433, Peoples R China.

Baio Technol Ltd Co, Shanghai 200233, Peoples R China.

语种：

英文

关键词：

Mycobacterium tuberculosis;drug resistance;biochip;enzymatic color reaction;mixed sample;templex PCR

期刊：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

ISSN：

1017-7825

年：

2015

卷：

期：

页码：

180-189

DOI：

10.4014/jmb.1501.01015

基金类别：

National Key Technologies R & D Program of ChinaNational Key Technology R&D Program [2013ZX10003003-004002]; Shanghai Science and Technology CommissionScience & Technology Commission of Shanghai Municipality (STCSM)Shanghai Science & Technology Committee [12XD1422000, 13DZ2252000]; Educational Commission of Hubei China [D20121809]

机构署名：

本校为其他机构

院系归属：

生命科学与技术学院

摘要：

The widespread occurrence of drug-resistant Mycobacterium tuberculosis places importance on the detection of TB (tuberculosis) drug susceptibility. Conventional drug susceptibility testing (DST) is a lengthy process. We developed a rapid enzymatic color-reaction-based biochip assay. The process included asymmetric multiplex PCR/templex PCR, biochip hybridization, and an enzymatic color reaction, with specific software for data operating. Templex PCR (tem-PCR) was applied to avoid interference between different primers in conventional multiplex-PCR. We applied this assay to 276 clinical specime...

反馈

产权有误：本人成果被他人认领

数据有误：数据基本信息有误

归属有误：成果的院系归属、机构署名归属有误

其他原因：

验证码：

看不清楚，换一个

确定

取消

成果认领

标题：

用户	作者	通讯作者	--
	请选择	请选择	--

确定

取消

A Color-Reaction-Based Biochip Detection Assay for RIF and INH Resistance of Clinical Mycobacterial Specimens

反馈

成果认领

提示

该栏目需要登录且有访问权限才可以访问