期刊论文

中文

中国兽药杂志

1002-1280

2007

41

9

13-16

10.3969/j.issn.1002-1280.2007.09.004

本校为第一机构

采用戊二醛交联法和混合酸酐法制备了四环素-BSA完全抗原,分别将两种不同偶联法制备的四环素-BSA完全抗原按照常规免疫程序免疫新西兰大白兔制备多克隆抗体,酶联免疫吸附法鉴定出多抗中含有四环素的特异性抗体,特异性抗体效价最高达1:320 000,抑制率为50%(IC50)时的四环素浓度为92.6 μg/mL,四环素的最低检测限为6.25 μg/mL,四环素和金霉素的交叉反应率达到78%,该方法灵敏度高,为试纸条法快速检测四环素类的残留奠定了基础.

Tetracycline-BSA immunoantigens （TC-BSA）were prepared via giutaraldehyde method and succinic acid method, then the different immunoantigens were injected into rabbits in order to prepare different polyclonal antibodies. The special antibody of Tetracycline（TC） was identified by indirect enzyme -linked immunosorbent assay （ELISA）. It was proved that the working concentration of the two kinds of the special antibodies was 1：320 000, the Tetracycline （ TC ） inhibition concentration （ IC50 ） was 92.6 μg/mL, the detection limit was 6.25 ...