期刊论文

Effects of Lipopolysaccharide on the m RNA Expression of the Key Genes and Negative Regulatory Factors in NODs Signaling Pathway in the Tissues of Intestines,Liver and Muscle of Piglets

中文

中国畜牧杂志

0258-7033

2016

52

11

35-38,54

10.3969/j.issn.0258-7033.2016.11.008

湖北省教育厅优秀中青年科技创新团队项目（T201508）；

本校为第一机构

本试验旨在研究脂多糖（LPS）刺激对仔猪核苷酸结合寡聚化结构域蛋白（NODs）信号通路关键基因及其负调控因子m RNA表达的影响。选取12头杜×长×大断奶仔猪,分成2个处理组,每个处理组6个重复。试验组注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后,屠宰仔猪,取空肠、回肠、肝脏、背最长肌和腓肠肌,应用实时荧光定量PCR技术测定NODs信号通路关键基因及其负调控因子m RNA表达水平,包括NOD1、NOD2、受体相互作用蛋白激酶2（RIPK2）、核因子-κB（NF-κB）、肿瘤坏死因子-α（TNF-α）、矢车菊苷β1（ACAP1）和Erbb2相互作用蛋白（ERBB2IP）m RNA表达水平。结果表明:与...

This experiment was conducted to investigate the effects of lipopolysaccharide（LPS） challenge on the m RNA expression of the key genes and negative regulatory factors in nucleotide-binding oligomerization domain proteins（NODs）signaling pathway in the tissues of intestines,liver and muscles of piglets.Twelve crossbred（Duroc ×Large ×White Landrace） piglets were allotted to two groups and 6 replicates per treatment.The piglets in the treatment group were injected with LPS at 100 μg/kg body weight,whereas piglets in the control group were ...