期刊论文

Effect of Necrostatin-1 on Blood Differential Count and Blood Biochemical Measurements of Piglets Challenged with Lipopolysaccharide

中文

中国畜牧杂志

0258-7033

2018

54

8

80-86

10.19556/j.0258-7033.2018-08-080

国家自然科学基金面上项目（31772615） 湖北省教育厅优秀中青年科技创新团队项目（T201508） 杭州市钱江特聘专家（3122）

本校为第一机构

本试验旨在研究程序性坏死阻断剂Necrostatin-1 (Nec-1)对脂多糖(LPS)刺激断奶仔猪血细胞分类计数和血液生化指标的影响.选用25头断奶仔猪,分为对照组[10％二甲基亚砜(DMSO)+生理盐水]、LPS组(10％DMSO+LPS)、0.33Nec-1组[0.33 mg/kg体重(BW) Nec-1+LPS]、1.0Nec-1组(1.0 mg/kg BW Nec-1+LPS)和3.3Nec-1组(3.3 mg/kg BW Nec-1+LPS).注射10％ DMSO或Nec-1 30 min后,对照组注射生理盐水,其他组注射100 μg/kg BW LPS,分别在注射LPS或生理盐水4h和24h后采血液样品待测.结果表明:注射LPS 4h后,0.33 mg/kg BW Nec-1、1.0 mg/kg BW Nec-1和3.3 mg/kg BW Nec-1显著缓解LPS刺激导致的血小板分布宽度升高...

The study was conducted to investigate the effects of Necrostatin-1（Nec-1） on blood cell differential count and blood biochemical measurements of piglets challenged with lipopolysaccharide（LPS）. Twenty-five piglets were randomly assigned into 5 groups： control; LPS; LPS＋0.33 Nec-1（0.33 mg/kg BW）; LPS＋1.0 Nec-1（1.0 mg/kg BW）; LPS＋3.3 Nec-1（3.3 mg/kg BW）. After injection of 10%DMSO or Nec-1 for 30 mins, piglets were injected with either LPS or saline. At 4 h or 24 h after LPS or saline injection, blood samples were taken. The result...