期刊论文

Effects of Lipopolysaccharide Challenge on Genes Related to Muscle Inflammation and Protein Degradation of Weaned Piglets

中文

中国畜牧杂志

0258-7033

2019

55

4

74-77

10.19556/j.0258-7033.2019-04-074

湖北省教育厅优秀中青年科技创新团队项目（T201508）；

本校为第一机构

本试验旨在研究脂多糖(LPS)刺激后不同时间断奶仔猪肌肉炎症和肌肉蛋白质降解相关基因表达的变化规律.选择42头(7.1±0.9) kg杜×长×大三元杂断奶仔猪,按注射LPS之前(0 h)和注射LPS后1、2、4、8、12、24 h随机分为7个处理,每个处理6头猪.预试14d后,腹腔注射100 tg/kg体重的LPS.按以上时间点将仔猪屠宰,取背最长肌样品待测.结果 表明:背最长肌炎性细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6的mRNA表达量在注射LPS 1～2 h后达到峰值;Toll样受体4信号通路关键基因Toll样受体4(TLR4)、骨髓分化因子88 (MyD88),核苷酸结合寡聚域信号通路关键基因核苷酸结合寡聚域2 (NOD2)、受体...

The purpose of this study was to investigate the changes of genes related to muscle inflammation and protein degradation in weaned piglets at different time points after lipopolysaccharide(LPS)challenge.Forty-two weaned piglets(Duroc×Landrace×Yorkshine)with average body weight(7.1±0.9 kg)were randomly divided into seven groups.The piglets were injected with 100μg/kg BW LPS,and slaughtered at 0 h(before LPS challenge),1,2,4,8,12 and 24 h(after LPS challenge).The longissimus dorsi muscle samples were collected for analysis.The results showed ...