期刊论文

Establishment of IPEC1 Cell Injury Model with LPS and Determination of Related Indexes

中文

中国畜牧杂志

0258-7033

2020

56

8

140-145

10.19556/j.0258-7033.20190906-06

武汉市应用基础前沿项目（2018020401011304）；

本校为第一机构

本试验旨在研究脂多糖(LPS)刺激对猪肠上皮细胞系(IPEC1)细胞活力、乳酸脱氢酶(LDH)活性、紧密连接蛋白和炎症相关基因表达的影响.分别选取0、0.025、1、10 μg/mL的LPS刺激细胞1、2、4、8h,检测相关指标.结果 表明:在不同时间,不同浓度的LPS对细胞活力的影响未呈现出显著的规律性;与0、0.025 μg/mLLPS组相比,在1、4、8h,1、10 μg/mL LPS处理导致IPEC1上清液LDH活性显著升高;在不同时间,不同浓度的LPS对紧密连接蛋白(ZO-1、occludin、claudin-1)和炎症相关基因(TLR4、NF-κB、TNF-α和IL-6)mRNA表达影响未呈现出显著的规律性.以上结果表明,LPS刺激能引起IPEC1细胞损伤,但不能诱导显著的炎症反...

The experiment was conducted to investigate the effects of lipopolysaccharide(LPS) on IPEC1 cells. The IPEC1 cells were stimulated with the different doses(0 ng/mL, 25 ng/mL, 1 μg/mL and 10 μg/mL) of LPS at different times(1, 2, 4, 8 h). The results showed that different doses of LPS had no consistent effect on cell viability at different time. LPS challenge(1 μg/mL and 10 μg/mL) increased LDH activity at 1, 4 and 8 h. Different doses of LPS had no consistent effect on mRNA expression of tight junction proteins(ZO-1, occludin and claudin-1)...