期刊论文

中文

中国畜牧杂志

0258-7033

2020

02

84-87

10.19556/j.0258-7033.20190704-03

国家自然科学基金(31702203) 湖北省教育厅优秀中青年科技创新团队项目(T201508)。

本校为第一机构

本试验旨在研究脂多糖(LPS)诱导仔猪发生炎症反应时肝脏和脾脏炎性细胞因子和lncRNA-47491、lncRNA-49770、lncRNA-51636表达的变化。选取8头21日龄、体重(7.15±0.42)kg的杜×长×大三元杂交断奶仔猪,随机分为对照组、LPS组,每个处理4个重复,每个重复1头猪。预试14 d后,LPS组和对照组腹腔分别注射100μg/kg体重的LPS、生理盐水,4 h后屠宰并取肝脏和脾脏组织样待测。结果表明:与对照组相比,LPS刺激使肝脏和脾脏中炎性细胞因子的mRNA表达量显著上调;LPS诱导的炎症反应中lncRNA-47491、lncRNA-49770和lncRNA-51636的表达量均显著上调;对lncRNA调控的顺式靶标基因的预测结果显示,lncRNA-47491和lnc...

The purpose of this study was to investigate the changes in the expression of inflammatory cytokines and lncRNA-47491,lncRNA-49770,and lncRNA-51636 in liver and spleen induced by lipopolysaccharide(LPS)in piglets.A total of 8 weaned piglets(Duroc×Landrace×Yorkshine;weaned at 21 d of age;(7.15±0.42)kg initial body weight)were randomly divided into two groups(n=4),including control group and LPS group.After 14 days of pretest,the piglets in LPS group were injected with 100μg/kg BW LPS and the piglets in control group were injected with an equ...