Protein phosphatase ( PPP) family was involved in regulating in vivo a variety of important physiological functions, including cell cycle, cell growth and differentiation processes.In this paper, we aligned sequences of PPP genes which play an important role in the signal transduction, to study evolution of PPP gene family, among Homo, Suidae, Rattus, Mus, and Gorilla The results show that ppp1r3a gene sequence was late differentiation, and slightly conservative, whilst the other protein phosphatase gene family sequences were earlier differe...