作者： Liu, Yang;Li, Qi;Wang, Hualin*;Zhao, Xiuju（赵秀举）;Li, Na;...

期刊： JOURNAL OF NUTRITIONAL BIOCHEMISTRY,2019年69:53-62 ISSN：0955-2863

通讯作者： Wang, Hualin

作者机构： [Zhao, Xiuju; Wang, Hualin; Li, Qi; Liu, Zhiguo; Li, Na; Liu, Yang; Zhang, Hongyu] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Hubei Prov Engn Res Ctr Hlth Food, Wuhan 430023, Hubei, Peoples R China.;[Chen, Guoxun] Univ Tennessee, Dept Nutr, Knoxville, TN 37996 USA.

通讯机构： [Wang, Hualin] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Hubei Prov Engn Res Ctr Hlth Food, Wuhan 430023, Hubei, Peoples R China.

关键词： acetylcarnitine;aconitic acid;alanine;arginine;aspartic acid;bile acid;dextro glutamic acid;dicarboxylic acid;fish oil;glutamine;glyoxylic acid;high density lipoprotein cholesterol;histidine;homoserine;hypotaurine;kynurenine;lauric acid;linolenic acid;phenylalanine;proline;sebacic acid;starch;sucrose;taurine;tryptophan;tyrosine;bile acid;fish oil;amino acid metabolism;animal experiment;animal model;animal tissue;Article;bile acid synthesis;bile composition;C57BL 6 mouse;circadian rhythm;controlled study;dietary supplement;down regulation;fatty acid oxidation;fatty acid synthesis;high density lipoprotein cholesterol level;lipid diet;lipid storage;liquid chromatography-mass spectrometry;liver hypertrophy;liver tissue;male;metabolomics;mouse;nonalcoholic fatty liver;nonhuman;obesity;protein expression;retention time;sucrose metabolism;time of flight mass spectrometry;upregulation;Western blotting;adverse event;animal;C57BL mouse;drug effect;liver;metabolism;multivariate analysis;nonalcoholic fatty liver;pathology;signal transduction;sleep disorder;Animals;Bile Acids and Salts;Chronobiology Disorders;Diet, High-Fat;Dietary Supplements;Fish Oils;Liver;Male;Mice, Inbred C57BL;Multivariate Analysis;Non-alcoholic Fatty Liver Disease;Signal Transduction

摘要： Our previous studies have found that fish oil rich in omega-3 polyunsaturated fatty acids (omega-3 PUFA) protects against non-alcoholic fatty liver disease (NAFLD) in mice. This study was aimed to explore the effects of fish oil on high fat diet (HFD)-induced circadian bile composition chaos. Male C57BL/6 mice were randomly divided into three groups, a control group (CON), a HFD group and a fish oil (FO) group, which were fed a normal chow diet, a HFD, and a HFD supplemented with FO, respectively for 12 weeks. At the end of the experiment, liver tissue, blood and bile samples were processed at 12-h intervals with the first one at zeitgeber time 0 (ZT0) and the second at zeitgeber time 12 (ZT12). Metabolites in bile were determined using UPLC-QTOF-MS, screened using multivariate statistical analysis, and analyzed using KEGG database and Metaboanalyst. The expression levels of key proteins in bile acid metabolism were examined using western blot. Results of biochemical analysis and H&E staining illustrated that feeding of HFD induced NAFLD, which was ameliorated in FO group. The bile content of each group at ZT0 (CON, HFD, or FO group) was respectively higher than that at ZT12 (P<.05). The metabolic pathway analysis of differential metabolites showed that these differences were correlated with amino acid metabolism, fatty acid biosynthesis and primary bile acid synthesis at ZT0. FO supplement could modify bile composition, which was related to the influence of its omega-3 PUFA on liver metabolism. omega-3 PUFA may also regulate the circadian rhythm of bile metabolism.

语种： 英文

作者： 刘杨;阮梦成;赵秀举（赵秀举）;王华林;刘志国

期刊： 武汉轻工大学学报,2019年38(2):21-24,30 ISSN：2095-7386

作者机构： 武汉轻工大学生物与制药工程学院,湖北武汉,430023;[赵秀举; 阮梦成; 刘杨; 王华林; 刘志国] 武汉轻工大学

关键词： 高脂饮食;虾青素;胆汁代谢

摘要： 研究虾青素对高脂高胆固醇饲喂小鼠胆汁成分的调节作用.采用高脂高胆固醇饲喂小鼠模型,设置高脂高胆固醇饲喂组、高脂高胆固醇添加虾青素组,12周后测定小鼠血浆总甘油三酯(TG)、总胆固醇(TC),HE染色观察肝组织形态学变化,LC-MS测定胆汁组成.结果 显示:高脂高胆固醇饮食增补虾青素能够降低小鼠脂肪指数、血浆TG和TC;此外,高脂高胆固醇饮食小鼠在添加虾青素后胆汁部分成分有明显变化,其中高脂所致的组氨醇、组氨酸等成分有所改善.结论:添加虾青素能够对高脂高胆固醇饮食小鼠胆汁成分起到明显调节作用.

语种： 中文

作者： Hu, Xiao-Yu;Li, Yun;Li, Long-Quan;Zheng, Yuan;Lv, Jia-Hong;...

期刊： BMJ OPEN,2018年8(4):e019974 ISSN：2044-6055

通讯作者： Zhao, Xiu-Ju（赵秀举）

作者机构： [Zhao, Xiu-Ju; Li, Yun; Li, Long-Quan; Lv, Jia-Hong; Huang, Shu-Chun; Zhang, Weinong; Zhao, Ling; Liu, Liang; Liu, Zhuiguo; Hu, Xiao-Yu; Zheng, Yuan] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan, Hubei, Peoples R China.;[Zhao, Xiu-Ju] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA.

通讯机构： [Zhao, Xiu-Ju] W;[Zhao, Xiu-Ju] C;[Zhao, Xiu-Ju] T;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan, Hubei, Peoples R China.

关键词： epidemiology;hepatology;public health

摘要： Objective Non-alcoholic fatty liver disease (NAFLD) is a major public health burden in China, and its prevalence is increasing. This study aimed to determine the risk factors and biomarkers of NAFLD. Design An observational cross-sectional primary survey. Setting Central China. Participants The study included 1479 participants aged over 18 and below 80 years, not currently being treated for cancer or infectious disease or no surgery in the previous year, and no history of cancer or an infectious disease. Participants underwent clinical examination, metabolomic assay and anthropometric assessment. Univariate and logistic regression analyses were used to evaluate associations between covariates and NAFLD. Main outcome measures Risk factors and metabolic biomarkers including sex, body mass index, hypertension, body fat ratio, blood triglycerides, blood fasting glucose, liver enzyme elevation, uric acid and oleic acid-hydroxy oleic acid (OAHOA). Results Data from the 447 participants (mean age 44.3±11.9 years) were analysed, and the prevalence of NAFLD was 24.7%. Male sex (OR 3.484, 95% CI 2.028 to 5.988), body mass index ≥24 kg/m 2 (OR 8.494, 95% CI 5.581 to 12.928), body fat ratio (≥25 for women, ≥20 for men) (OR 1.833, 95% CI 1.286 to 2.756), triglycerides ≥1.7 mmol/L (OR 1.340, 95% CI 1.006 to 1.785), fasting glucose ≥6.1 mmol/L (OR 3.324, 95% CI 1.888 to 5.850), blood pressure ≥140/90 mm Hg or antihypertensive drug treatment (OR 1.451, 95% CI 1.069 to 1.970), uric acid (≥357 μmol/L for women, ≥416 μmol/L for men) (OR 2.755, 95% CI 2.009 to 3.778) and OAHOA (<5 nmol/L) (OR 1.340, 95% CI 1.006 to 1.785) were independent predictors of NAFLD (all P<0.05). These results were verified by all 1479 participants. Conclusions NAFLD was common among the study participants. In particular, NAFLD was correlated with uric acid. We identified OAHOA as a novel marker of NAFLD prevalence. It provides a reference on the prevention of NAFLD and related metabolic diseases with the rapid urbanisation, technological advancement and population ageing in China over the recent decades. © 2018 Article author(s) (or their employer(s) unless otherwise stated in the text of the article). All rights reserved.

语种： 英文

作者： Chen, Yu-Lian;Xiao, Chuan-Hao;Hu, Zhi-Xiong;Liu, Xiao-Shan;Liu, Zhiguo;...

期刊： JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017年1055-1056:165-171 ISSN：1570-0232

通讯作者： Zhao, Xiu-Ju（赵秀举）;Zhang, Wei-Nong

作者机构： [Zhao, Xiu-Ju; Hu, Zhi-Xiong; Liu, Zhiguo; Liu, Xiao-Shan; Chen, Yu-Lian] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan 430062, Peoples R China.;[Zhang, Wei-Nong] Wuhan Polytech Univ, Sch Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;[Xiao, Chuan-Hao] Puyang Vocat & Tech Coll, Puyang 457000, Peoples R China.

通讯机构： [Zhao, Xiu-Ju; Zhang, Wei-Nong] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Sch Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.

关键词： Biochemistry;Biomarkers;Body fluids;Gas chromatography;Mammals;Mass spectrometry;Monounsaturated fatty acids;Spectroscopy;Unsaturated fatty acids;Clinical biochemistry;Control groups;High-fat diet;Hyperlipidemia;Lipid profile;Mass spectroscopy;Serum samples;Time points;Fatty acids;alanine aminotransferase;aspartate aminotransferase;cholesterol;glucose;monounsaturated fatty acid;polyunsaturated fatty acid;saturated fatty acid;unsaturated fatty acid;biological marker;fatty acid;alanine aminotransferase blood level;animal experiment;animal model;animal tissue;Article;aspartate aminotransferase blood level;cholesterol blood level;controlled study;fatty acid blood level;glucose blood level;hyperlipidemia;lipid diet;male;mass fragmentography;mouse;nonhuman;pathology;priority journal;adverse effects;animal;blood;chemistry;Hyperlipidemias;liver;procedures;Animals;Biomarkers;Diet, High-Fat;Fatty Acids;Gas Chromatography-Mass Spectrometry;Hyperlipidemias;Liver;Male;Mice

摘要： Biomarkers of serum fatty acids in hyperlipidemia need to be elucidated. 90 SPF KM male mice were randomly divided into 18 groups (n=5/group), control groups, and high fat diet (HFD) groups at 9 time points. On day 7, 10, 15, 18, 21, 24, 28, 31, and 35, the mice were sacrificed; blood was collected into tubes from the eyes, serum samples for clinical biochemistry assays and gas chromatography–mass spectroscopy were attained after centrifugation, and the contents of serum fatty acids were detected with GC–MS. Sections of livers were taken and stored in formalin solution for histological assessments. No species differences existed in all these groups. The contents of C16:1, C18:1, C22:6 were significantly different between HFD groups and the corresponding controls; meanwhile, the proportion of fatty acids, especially the monounsaturated degree, the polyunsaturated degree, changed significantly and regularly (P<0.05). Thus the three unsaturated fatty acids C16:1, C18:1, C22:6 and the monounsaturated/polyunsaturated unsaturated degrees may be as potential biomarkers of hyperlipidemia. © 2017 Elsevier B.V.

语种： 英文

作者： Zhao, Xiu-Ju*（赵秀举）;Chen, Yu-Lian;Fu, Bing;Zhang, Wen;Liu, Zhiguo*;...

期刊： Journal of the Science of Food and Agriculture,2017年97(4):1158-1163 ISSN：0022-5142

通讯作者： Zhao, Xiu-Ju（赵秀举）;Liu, Zhiguo

作者机构： [Zhao, Xiu-Ju; Liu, Zhiguo; Chen, Yu-Lian] Wuhan Polytech Univ, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd,Changqing Garden, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan 430062, Peoples R China.;[Fu, Bing] Changyuan Cuisine Vocat & Tech Coll, Changyuan 453400, Peoples R China.;[Zhang, Wen] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China.;[Zhuo, Hexian] Xinxiang Inst Drug Control Food & Drug Adm Xinxia, 17 Jiankang Rd, Xinxiang 453000, Peoples R China.

通讯机构： [Zhao, XJ; Liu, ZG] W;Wuhan Polytech Univ, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd,Changqing Garden, Wuhan 430023, Peoples R China.

关键词： amino acid;choline;fat intake;glucose;nucleotide;vegetable oil;adverse effects;animal;chemistry;drug effects;endoplasmic reticulum stress;fat intake;genetic transcription;lipid diet;liver;Metabolic Diseases;metabolism;metabolome;metabolomics;plant seed;real time polymerase chain reaction;squash;unfolded protein response;Wistar rat;Amino Acids;Animals;Choline;Cucurbita;Diet, High-Fat;Dietary Fats;Endoplasmic Reticulum Stress;Glucose;Liver;Metabolic Diseases;Metabolome;Metabolomics;Nucleotides;Plant Oils;Rats, Wistar;Real-Time Polymerase Chain Reaction;Seeds;Transcription, Genetic;Unfolded Protein Response

摘要： BACKGROUND: Understanding the metabolic and transcription basis of pumpkin seed oil (PSO) intervention on metabolic disease (MD) is essential to daily nutrition and health. RESULTS: This study analyzed the liver metabolic variations of Wistar rats fed normal diet (CON), high-fat diet (HFD) and high-fat plus PSO diet (PSO) to establish the relationship between the liver metabolite composition/transcript profile and the effects of PSO on MD. By using proton nuclear magnetic resonance spectroscopy together with multivariate data analysis, it was found that, compared with CON rats, HFD rats showed clear dysfunctions of choline metabolism, glucose metabolism and nucleotide and amino acid metabolism. Using quantitative real-time polymerase chain reaction (qPCR), it was found that, compared with HFD rats, PSO rats showed alleviated endoplasmic reticulum stress accompanied by lowered unfolded protein response. CONCLUSION: These findings provide useful information to understand the metabolic alterations triggered by MD and to evaluate the effects of PSO intervention. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry

语种： 英文

作者： 丁洪波;赵秀举（赵秀举）

期刊： 食品工业科技,2015年36(12):341-343 ISSN：1002-0306

作者机构： [赵秀举; 丁洪波] 武汉轻工大学生物与制药工程学院;农产品加工湖北省协同创新中心

关键词： 高血脂;血清脂肪酸标志物;气质联用

摘要： 为了筛选南瓜籽油降脂效果的生物标志物,将36只SPF级KM系雄性小鼠随机分为对照组、高脂组、南瓜籽油.干预组。喂养4周后处死,使用气相色谱-质谱检测血清脂肪酸的种类与含量。结果表明,各组小鼠血清脂肪酸种类无差别;而脂肪酸C16:1、C18:1、C18:2、C22:6的含量变化显著(p<;0.05)。因此,小鼠血清脂肪酸C16:1、C18:1、C18:2、C22:6可做为南瓜籽油降脂的生物标志物。

语种： 中文

作者： 赵秀举（赵秀举）

期刊： 中国生物化学与分子生物学报,2015年31(5):543-547 ISSN：1007-7626

作者机构： 武汉轻工大学生物与制药工程学院,农产品加工湖北省协同创新中心,武汉430023;[赵秀举] 武汉轻工大学

关键词： 人端粒酶活性;有限延伸法;错配

摘要： 人端粒酶是一种核蛋白体,通过其内含的RNA模板与端粒末端配对把重复端粒片段添加在端粒3'末端; 因此,端粒酶活性与细胞凋亡、衰老、永生化有密切关系,是癌症临床预测诊断的一个生物标签. 现有的端粒酶活性检测方法,存在灵敏度低和不易定量等问题. 本研究采用错配有限延伸法检测端粒酶活性:在人端粒酶延伸人工合成的游离端粒酶底物时,只加入dATP和dGTP,端粒酶只能把底物延伸4个脱氧核糖核苷酸AGGG. 然后加入dNTP,让端粒酶延伸的产物和一条长的引物配对从而延伸出PCR模板; 再加入引物进行热启动PCR. PCR后进行非变性PAGE(polyacrylamide gel electrophoresis), 得到希望的唯一1条目标带. 同时,用不同的端粒酶浓度梯度进行优化,发现有限延伸法检测端粒酶活性的下限达到250个HeLa细胞.

语种： 中文

作者： 彭芳;郭世新;赵秀举（赵秀举）

期刊： 武汉轻工大学学报,2015年(2):40-42,54 ISSN：2095-7386

作者机构： 武汉轻工大学生物与制药工程学院,湖北武汉,430023;[赵秀举; 彭芳; 郭世新] 武汉轻工大学

关键词： 蛋白磷酸酶;PPP基因家族;基因比对

摘要： 蛋白磷酸酶（ PPP）家族在体内参与调节多种重要的生理功能，包括细胞周期、细胞生长及分化等过程。通过对在信号转导中起着重要作用的PPP基因进行序列比对，研究人、猪、大鼠、小鼠以及大猩猩的PPP基因家族的进化。结果表明：除了ppp1r3a 基因序列分化较晚，基因保守性略高外，其它蛋白磷酸酶基因家族序列分化较早，分支较大，但在分化后保守性高。

语种： 中文

作者： 刘小珊;赵秀举（赵秀举）;胡志雄;齐玉堂;张维农

期刊： 武汉轻工大学学报,2015年(3):25-30 ISSN：2095-7386

作者机构： 武汉轻工大学食品科学与工程学院,湖北武汉,430023;[赵秀举; 张维农; 刘小珊; 齐玉堂; 胡志雄] 武汉轻工大学

关键词： 高脂血症;血清脂肪酸;气质联用;不饱和脂肪酸

摘要： 探讨高脂血症小鼠血清脂肪酸水平的变化。以高脂血症小鼠为模型,以生化检测指标为基础,通过GC—MS检测技术监测基础组、高脂组及配方油脂干预组小鼠血清脂肪酸的差异。将50只SPF级KM系雄性小鼠随机分为基础组、高脂组、配方油低中高剂量组。连续喂养4周,提取小鼠血清,检测血清生化指标,并使用气相色谱—质谱联用技术检测其脂肪酸的种类与含量。结果表明,成功建立高脂血症小鼠模型,且小鼠血清脂肪酸种类并无差别;而与基础组比较,高脂组C14∶0、C16∶1、C18∶1、C22∶6的含量有显著性差异;与高脂组相比,配方油组各脂肪酸含量均有不同程度变化,其中C16∶1、C18∶1、C22∶6的含量变化趋势显著（P〈0.05）;且不饱和脂肪酸比例在各组之间也有显著性差异。根据GC—MS（气质联用）监测结果可知,C16∶1、C18∶1、C22∶6三种脂肪酸在各组之间变化显著,与高脂血症代谢异常有关,血清不饱和脂肪酸比例与高脂血症关系密切。

语种： 中文

作者： 王杰;王丽梅;赵秀举（赵秀举）;张继文;刘烈炬

期刊： 中国油脂,2015年40(2):40-45 ISSN：1003-7969

作者机构： 武汉轻工大学生物与制药工程学院;[赵秀举; 张继文; 刘烈炬; 王丽梅] 农产品加工湖北省协同创新中心;[王杰] 武汉轻工大学

关键词： 油脂;大鼠;空间学习记忆;Morris水迷宫

摘要： 研究了DHA油、核桃油、红花油、紫苏油以及亚油酸对初断乳大鼠空间学习记忆的影响,并探讨其与ASICs(酸敏感离子通道)基因表达的关系。将48只初断乳的SD大鼠随机分成6组:阴性对照组(基础饲料)、DHA油组(0.902 mL/(kg·d))、核桃油组(2.445 mL/(kg·d))、红花油组(1.792 mL/(kg·d))、紫苏油组(2.445 mL/(kg·d))与亚油酸组(1.464 mL/(kg·d))。油样经口灌胃,连续喂养8周,第8周进行Morris水迷宫行为学测试,并用RT-PCR检测大鼠海马组织中ASICs的mRNA表达情况。结果表明:与阴性对照组比较,DHA油组、核桃油组、红花油组、紫苏油组和亚油酸组的逃避潜伏期明显缩短(P<0.05),而穿越平台次数均显著增加(P<0.05);与阴性对照组相比,DHA油、核桃油、红花油、紫苏油和亚油酸能显著提高大鼠脑部海马组织中的ASIC4 mRNA的表达量(P<0.05),而使ASIC1a、ASIC1b mRNA的表达量显著(P<0.05)或极显著(P<0.01)降低;DHA油能显著提高ASIC3 mRNA的表达量(P<0.05);核桃油能显著提高ASIC2b mRNA的表达量(P<0.05);红花油能极显著提高ASIC2b mRNA的表达量(P<0.01);紫苏油能显著提高ASIC2b mRNA的表达量(P<0.05),极显著提高ASIC2a mRNA的表达量(P<0.01);亚油酸能极显著提高ASIC2a mRNA的表达量(P<0.01)。因此,DHA油、核桃油、红花油、紫苏油和亚油酸能够提高大鼠的空间学习记忆能力;不同油脂提高大鼠空间学习记忆能力的过程与ASICs 亚基 mRNA的表达水平有关。

语种： 中文

作者： 罗申军;白璐;赵秀举（赵秀举）

期刊： 武汉轻工大学学报,2015年34(1):14-16 ISSN：2095-7386

作者机构： 武汉轻工大学生物与制药工程学院,湖北武汉,430023;[赵秀举; 白璐; 罗申军] 武汉轻工大学

关键词： 南瓜多糖;提取;优化

摘要： 用三因素三水平正交试验,对影响南瓜多糖提取率的因素:提取时间、提取温度和料液比进行了优化研究。结果显示,影响提取率的主要因素分别为提取温度>料液比>提取时间。最优提取条件为:提取温度为70℃、提取时间为2 h、料液比为1∶2。多糖的最高得率为1.9%。

语种： 中文

作者： Yang, Jiang-Ke*;Xiong, Wei;Xu, Li;Li, Jia;Zhao, Xiu-Ju（赵秀举）

期刊： Enzyme and Microbial Technology,2015年75-76:64-70 ISSN：0141-0229

通讯作者： Yang, Jiang-Ke

作者机构： [Zhao, Xiu-Ju; Xu, Li; Li, Jia; Yang, Jiang-Ke; Xiong, Wei] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

通讯机构： [Yang, Jiang-Ke] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

关键词： CO differential display;Constitutive promoter;CtrHb;Growth curve;Semi-quantitative PCR

摘要： Bacteria hemoglobin could bind to the oxygen, transfer it from the intracellular microenvironment to the respiration process and sustain the energy for the metabolism and reproduction of cells. Heterologous expression of bacteria hemoglobin gene could improve the capacity of the host on oxygen-capturing and allow it to grow even under microaerophilic condition. To develop a system based on hemoglobin to help bacteria cells overcome the oxygen shortage in fermentation, in this study, Campylobacter jejuni truncated hemoglobin (CtrHb) gene was synthesized and expressed under the control of constitutive expression promoters P2 and PSPO1-II in Escherichia coli. As showed by the growth curves of the two recombinants P2-CtrHb and PSPO1-II-CtrHb, constitutive expression of CtrHb improved cell growth under aerobic shaking-flasks, anaerobic capped-bottles and bioreactor conditions. According to the NMR analysis, this improvement might come from the expression of hemoglobin which could boost the metabolism of cells by supplying more oxygen to the respiratory chain processes. Through semi-quantitative RT-PCR and CO differential spectrum assays, we further discussed the connection between the growth patterns of the recombinants, the expression level of CtrHb and oxygen binding capacity of CtrHb in cells. Based on the growth patterns of these recombinants in bioreactor, a possible choice on different type of recombinants under specific fermentation conditions was also suggested in this study. © 2015 Elsevier Inc.

语种： 英文

作者： Xiu-Ju Zhao（赵秀举）;Zhiguo Liu

作者机构： [Xiu-Ju Zhao; Zhiguo Liu] School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University;[Xiu-Ju Zhao; Zhiguo Liu] Wuhan Magnetic Resonance Center;[Xiu-Ju Zhao; Zhiguo Liu] Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences

会议名称： 2014年第十二届全国脂质与脂蛋白学术会议

会议时间： 2014-10-10

会议地点： 杭州

会议主办单位： 中国生物化学与分子生物学会脂质与脂蛋白专业委员会

会议论文集名称： 2014年第十二届全国脂质与脂蛋白学术会议论文集

摘要： <正>A food supplement is a complex multicomponent system.Previous researches of food supplements mainly focused on roles and actions of choline,taurine,carnitine,inositol,omega-3 polyunsaturated fatty acids（PUFAs,e.g.ALA,EPA,DPA,DHA）,omega-6 PUFAs（e.g.ARA）and long-chain saturated fatty acids（SFAs）.However,short-and medium-chain SFAs,sphingolipids,and the whole profile/spectrum are less concerned.

语种： 英文

作者： Wang Hualin;Yuan Fahu;Zhao Xiuju（赵秀举）;Zhang Hongyu;Liu Zhiguo

作者机构： [Zhao Xiuju; Wang Hualin; Yuan Fahu; Zhang Hongyu; Liu Zhiguo] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University

会议名称： 2014年第十二届全国脂质与脂蛋白学术会议

会议时间： 2014-10-10

会议地点： 杭州

会议主办单位： 中国生物化学与分子生物学会脂质与脂蛋白专业委员会

会议论文集名称： 2014年第十二届全国脂质与脂蛋白学术会议论文集

摘要： <正>Background:The advances in high-throughput technologies have allowed for the massive analysis of genomic data,providing benefits for the characterization of transcriptome architectures.Recent studi

语种： 英文

作者： Wang Hualin;Tian yu;Zhang Hongyu;Zhao Xiuju（赵秀举）;Liu Zhiguo

作者机构： [Zhao Xiuju; Wang Hualin; Tian yu; Zhang Hongyu; Liu Zhiguo] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University

会议名称： 2014年第十二届全国脂质与脂蛋白学术会议

会议时间： 2014-10-10

会议地点： 杭州

会议主办单位： 中国生物化学与分子生物学会脂质与脂蛋白专业委员会

会议论文集名称： 2014年第十二届全国脂质与脂蛋白学术会议论文集

语种： 英文

作者： 王丽梅;赵秀举（赵秀举）;刘志国;刘烈炬

作者机构： [赵秀举; 王丽梅; 刘志国; 刘烈炬] 武汉轻工大学生物与制药工程学院

会议名称： 2014年第十二届全国脂质与脂蛋白学术会议

会议时间： 2014-10-10

会议地点： 杭州

会议主办单位： 中国生物化学与分子生物学会脂质与脂蛋白专业委员会

会议论文集名称： 2014年第十二届全国脂质与脂蛋白学术会议论文集

摘要： <正>目的:研究不同剂量的紫苏油对大鼠学习记忆的影响及对海马组织中ASICs亚型(ASIC1a、ASIC1b、ASIC2a、ASIC2b、ASIC3和ASIC4)基因表达的影响,探讨紫苏油提高大鼠学习记忆能力的量效关系及其促记忆可能的机制。方法:64只四周龄SD大鼠适应性喂养1周后按随机数字表法随机分为4组,每组16只,紫苏油小剂量组(0.815ml/kg)、紫苏油中剂量组(2.445ml/kg)

语种： 中文

作者： 赵秀举（赵秀举）;刘志国

期刊： 中国酿造,2014年33(1):32-35 ISSN：0254-5071

作者机构： 武汉轻工大学生物与制药工程学院,农产品加工湖北省协同创新中心,湖北武汉430023;[赵秀举; 刘志国] 武汉轻工大学

关键词： 洛伐他汀;正交试验;红曲米;降脂

摘要： 以功能红曲为试验材料,采用正交试验设计优化发酵洛伐他汀（MK）的条件:高温温度及持续时间,低温温度及持续时间,装瓶体积,装料量。结果表明,高温35℃持续5d,低温22℃持续15d,装料量360g/L为发酵MK的优化条件。同时,通过细胞培养实验发现,功能红曲与MK通过缓解内质网应激具有降脂作用。

语种： 中文

作者： Zhao, Xiu-Ju*（赵秀举）;Zhuo, Hexian

期刊： BioMed Research International,2014年2014:850802 ISSN：2314-6133

通讯作者： Zhao, Xiu-Ju（赵秀举）

作者机构： [Zhao, Xiu-Ju] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China.;[Zhuo, Hexian] Xinxiang Inst Drug Control, Xinxiang 453000, Peoples R China.;[Zhao, Xiu-Ju] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.

通讯机构： [Zhao, Xiu-Ju] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.

摘要： Early growth is connected to a key link between embryonic development and aging. In this paper, liver gene expression profiles were assayed at postnatal day 22 and week 16 of age. Meanwhile another independent animal experiment and cell culture were carried out for validation. Significance analysis of microarrays, qPCR verification, drug induction/inhibition assays, and metabonomics indicated that alpha-2u globulin (extracellular region)-socs2 (-SH2-containing signals/receptor tyrosine kinases)-ppp2r2a/pik3c3 (MAPK signaling)-hsd3b5/cav2 (metabolism/organization) plays a vital role in early development. Taken together, early development of male rats is ECR and MAPK-mediated coordination of cancer-like growth and negative regulations. Our data represent the first comprehensive description of early individual development, which could be a valuable basis for understanding the functioning of the gene interaction network of infant development.

语种： 英文

作者： 赵秀举（赵秀举）;刘志国

期刊： 中国酿造,2013年32(11):36-38 ISSN：0254-5071

作者机构： 武汉轻工大学生物与制药工程学院 农产品加工湖北省协同创新中心,湖北武汉,430023;[赵秀举; 刘志国] 武汉轻工大学

关键词： 果糖1;6-二磷酸(FBP);正交试验;固定化;废旧酵母利用

摘要： 以酿酒酵母为试验材料,采用正交试验设计优化发酵果糖l,6-二磷酸(FBP)的条件:酵母添加量,蔗糖添加量,渗透剂比例,微量营养素含量.结果表明,酵母5％,蔗糖9％,甲苯、吐温-20各1％,MgCl2·6H2O 0.12％为发酵FBP的优化条件.在此基础上进行了酵母细胞破碎和固定化研究,使FBP含量得以进一步提高.同时活性干酵母发酵FBP的优化条件被应用到废旧酵母,提高了废旧酵母的利用价值.

语种： 中文

作者： 赵秀举（赵秀举）

期刊： 科技导报,2013年31(1):85 ISSN：1000-7857

作者机构： 武汉工业学院生物与制药工程学院;[赵秀举] 武汉工业学院

关键词： 教师;高校;工作岗位;工作经验

摘要： <正>高校年轻教师刚刚从培养单位毕业走上工作岗位,由于工作经验不足、资源缺乏等原因,在工作岗位上起步比较困难。注意以下几个方面,可能有助于更好地起步。1投入教学,站稳讲台教学是高校教师的主要任务之一。年轻教师要学习一些高等教育学、高等教育心理学的知识,将教育学理论运用到专业课程教学实践中去。多听老教师讲课,熟悉大学授课的流程,揣摩老教师是如何把握重点和难点、如何取舍教材内容的。在此基础上,年轻教师应参照已有的

语种： 中文