作者： Li, Yun;Zhao, Xiu-ju*

期刊： ANALYTICAL METHODS,2020年12(15):1995-2001 ISSN：1759-9660

通讯作者： Zhao, Xiu-ju

作者机构： [Li, Yun; Zhao, Xiu-ju] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;[Zhao, Xiu-ju] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.

通讯机构： [Zhao, Xiu-ju] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.

摘要： Hyperlipidemia dynamics need to be elucidated. 90 Kunming male mice were randomly divided into 18 groups, control groups, and high fat diet groups at 9 time points. On days 7, 10, 15, 18, 21, 24, 28, 31, and 35, the mice were anesthetized and blood was collected into tubes from the eyes. Sections of livers were taken. The plasma glycerol, glucose, leucine, arginine, betaine, lysine, glutamine, glutamate, valine, alanine, and choline were significantly different between the high fat diet groups and the corresponding controls (P < 0.05; VIP > 1). Therefore, the eleven metabolites may be used as potential biomarkers of hyperlipidemia. Based on the Spearman correlation analysis, it was found that valine was significantly associated with C22:6 from the 28th day to the end of the experiment, implying that these two metabolites may be a key point in subsequent research. These significantly affected metabolic pathways, namely arginine biosynthesis, d-glutamine and d-glutamate metabolism, alanine, aspartate and glutamate metabolism, were involved in the pathophysiological process of hyperlipidemia. © 2020 The Royal Society of Chemistry.

语种： 英文