期刊：
Environmental Science and Pollution Research,2024年:1-1 ISSN：0944-1344
通讯作者：
Sumin Hu
作者机构：
[Haoqiang Wu] School of Economics, Wuhan Polytechnic University, Wuhan, China;[Sumin Hu] Business School, Suzhou University of Science and Technology, Suzhou, China;[Sujie Hu] Business School, Suzhou University, Suzhou, China
通讯机构：
[Sumin Hu] B;Business School, Suzhou University of Science and Technology, Suzhou, China
期刊：
Journal of Flow Visualization and Image Processing,2024年31(1):33-52 ISSN：1065-3090
作者机构：
[Zunhai Gao] School of Information and Artificial Intelligence, Nanchang Institute of Science and Technology, Nanchang, 330108, China;[Hongtao Gao; Yuandong Xiang] School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, 430048, China;[Zunhai Gao] School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, 430048, China
摘要：
Existing deep learning methods for facial emotion recognition only focus on optimizing network structures, utilizing fixed receptive fields for different images, and relying on feature extraction based on a single scale of receptive fields. However, this approach fails to fully capture the most critical facial regions. To address this limitation, this paper presents a novel technique for facial emotion recognition that employs a selective kernel network. The proposed method introduces a dedicated module called the selective kernel network, which is trained using transfer learning. This module incorporates various components, such as a selective attention mechanism and channel-wise independent feature extraction and fusion. These components allow for the extraction of feature information from key facial regions. Unlike other methods, the selective convolutional kernel network extracts features with multiple scales of receptive fields and adapts to different spatial positions using a multilayer perceptron. This adaptability enhances useful features and suppresses noise. After extracting the features, they are combined, and the classification outcome is computed using the softmax function. Experimental results demonstrate that the suggested approach achieves an accuracy of 88.4 and 92.1% on the RAF-DB and KDEF datasets, respectively. These results confirm the efficacy of the proposed technique in comprehensively capturing the most crucial facial regions. Moreover, compared to alternative methods, this technique exhibits superior accuracy and enhanced resilience.
摘要：
This study utilises the transfer matrix method (TMM) to address the acoustic characteristics of multilayered cylindrical shells lined with porous materials. The TMM theoretical model for the sound transmission loss of composite cylindrical shells with internal porous materials is derived by establishing transfer matrices for the air/composite material interface, composite material/foam interface, foam/air interface and boundary interfaces. The accuracy of the TMM model is validated through a comparison and analysis with experimental results. Building upon this, the impact of porous foam material parameters and types on the structural sound transmission loss is discussed. The results indicate that the use of TMM accurately reflects the acoustic performance of composite structures. Additionally, this model allows for the determination of the influence patterns of porous foam material parameters and types on the acoustic performance of composite structures. In the frequency range of 100-10,000 Hz, the sound transmission loss of the melamine foam-lined composite structure increases with the increase in flow resistance and porosity and the decrease of the tortuosity factor. The use of the porous lining material significantly enhances the structural sound insulation performance.
摘要：
Fungal symbionts can improve plant tolerance to salt stress. However, the interaction of epiphytic Fusarium oxysporum and Fusarium fujikuroi with the tolerance of the invasive plant Ipomoea cairica against saline coastal habitats is largely unknown. This study aimed to investigate the interaction of the mixture of the two epiphytic fungi with salt tolerance of I. cairica. Surface-sterilized I. cairica cuttings inoculated (E+) and non-inoculated (E-) with the fungal mixture were cultivated with 2, 3, and 5 parts per thousand (PPT) of NaCl solutions to simulate mild, moderate, and severe salt stress, respectively. The hydroponic experiment showed that the growth inhibition and peroxidation damages of E+ and E- cuttings were aggravated with salinity. Noteworthily, E+ cuttings had higher peroxidase (POD) and catalase (CAT) activities, chlorophyll content, total biomass, aboveground biomass, total shoot length and secondary shoot number, but lower root-to-shoot ratio than E- cuttings under 2 and 3 PPT NaCl conditions. Moreover, E+ had higher superoxide dismutase (SOD) activity and proline content but lower belowground biomass and malondialdehyde (MDA) content than E- cuttings under 3 PPT NaCl condition. However, lower SOD, POD, and CAT activities, and chlorophyll content, but higher MDA content occurred in E+ cuttings than in E- cuttings under 5 PPT NaCl condition. These findings suggested that the mixture of the two epiphytic fungi increased salt tolerance of I. cairica mainly through increasing its antioxidation ability and chlorophyll stability under mildly and moderately saline conditions, but decreased salt tolerance of this plant in an opposite way under severely saline conditions.
期刊：
International Journal of Biological Macromolecules,2024年260(Pt 1):129329 ISSN：0141-8130
通讯作者：
Cai, J
作者机构：
[Cai, Jie; Yang, Zhaoxing; Zhang, Die] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Food packaging;Starch;Tannic acid
摘要：
Developing efficient and biodegradable packaging films is of paramount significance owing to the scarcity of petroleum based resources. However, their applications in food packaging are limited due to their poor mechanical properties and inadequate biological activities. This study proposes a novel approach to develop the starch composite nanofibrous films (SNFs/TA/Fe(3+)) consisting of starch, tannic acid, and Fe(3+) using the temperature-assisted electrospinning method. The addition of TA resulted in a decrease in the rate of thermal degradation, indicating an improvement in the thermal stability of SNFs. However, the incorporation of TA or TA/Fe(3+) showed only a slight impact on the internal structure of SNFs. SNFs/TA/Fe(3+) loaded with 0.1wt% of Fe(3+) demonstrated a significantly higher tensile strength compared to SNFs and those loaded with TA alone. The presence of TA enhances the antioxidant activity of SNFs, and the robust SNFs/TA/Fe(3+) exhibited comparable antioxidant activity to SNFs/TA. However, the SNFs/TA/Fe(3+) showed a reduction in antibacterial activity, possibly due to the high valence state of the metal ions. Overall, these findings highlighted that a simple electrospinning method was used to produce SNFs/TA/Fe(3+) resulted in improved mechanical properties and antioxidant activity, offering a new strategy for the development of active food packaging using SNFs.
作者机构：
[Hu, Tao; Dong, Hao; Wang, Guangjin; Gong, Xinghou; Wu, Chonggang; Dong, H; Guo, Lei; Zhu, Chuanhui; Zheng, Xuan; Wang, GJ] Hubei Univ Technol, Sch Mat & Chem Engn, Hubei Prov Key Lab Green Mat Light Ind, Wuhan 436800, Peoples R China.;[Wang, Guangjin] Foshan Univ, Sch Mat Sci & Energy Engn, Foshan 52800, Peoples R China.;[Dong, Hao] Zhongkai Univ Agr & Engn, Coll Light Ind & Food Technol, Guangzhou 510225, Peoples R China.;[Hu, Tao; Gong, Xinghou; Wu, Chonggang; Zheng, Xuan] Hubei Longzhong Lab, Xiangyang 441000, Peoples R China.;[Hou, Yuanjing] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430048, Peoples R China.
通讯机构：
[Dong, H ; Zheng, X; Wang, GJ] H;Hubei Univ Technol, Sch Mat & Chem Engn, Hubei Prov Key Lab Green Mat Light Ind, Wuhan 436800, Peoples R China.
关键词：
Ag nanowires;Biosensor;Food safety;MoS(2);Pesticide
摘要：
Thiabendazole (TBZ), a highly toxic phosphorothioate insecticide commonly used in postharvest fruit management, has the potential to cause detrimental effects on human health as an endocrine disruptor. In this study, an electrochemical sensor was developed to detect TBZ by modifying MoS(2) on silver nanowires (Ag NWs@MoS(2)) and integrating them onto a glassy carbon surface. Cyclic voltammetry revealed that TBZ underwent an irreversible, diffusion-controlled process on Ag NWs@MoS(2), leading to a two-fold increase in peak current compared to unmodified MoS(2). Square wave voltammetry facilitated TBZ detection, and the sensor exhibited a linear range of 0.05-10μM with a high coefficient of determination (R(2)=0.9958) and a limit of detection (LOD) of 1.75nM (signal-to-noise ratio=3). The sensor's applicability for food safety monitoring was verified through TBZ analysis in pear and apple samples, achieving recoveries of 95.5-103.6% with RSDs in the range of 1.98-3.25%.
作者机构：
[Yang, Sicheng; Liu, Jianfang; Peng, Shuai; Yi, Chenglingzi; Zhang, Yaoyun; Yang, Qing; Liu, Ting; Zhang, Rongrong] Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan 430023, Peoples R China.;[Jia, Dan] Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Peoples R China.
通讯机构：
[Liu, JF ] W;Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan 430023, Peoples R China.
摘要：
Anti-wear performance is a crucial quality of lubricants, and it is important to conduct research into the structure-activity relationship of anti-wear additives in bio-based lubricants. These lubricants are eco-friendly and energy-efficient. A literature review resulted in the construction of a dataset comprising 779 anti-wear properties of 79 anti-wear additives in rapeseed oil, at various loadings and additive levels. The anti-wear additives were classified into six groups, including phosphoric acid, formate esters, borate esters, thiazoles, triazine derivatives, and thiophene. Logistic regression analysis revealed that the quantity and kind of anti-wear agents had significant effects on the anti-wear properties of rapeseed oil, with phosphoric acid being the most effective and thiophene being the least effective. To identify the specific structural data that affect the anti-wear capabilities of additives in bio-based lubricants of rapeseed oil, a random forest classification model was developed. The results showed a 0.964 accuracy (ACC) and a 0.931 Matthews Correlation Coefficient (MCC) on the test set. The ranking of importance and characterization of MACCS descriptors in the model confirms that anti-wear additives with chemical structures containing P, O, N, S and heterocyclic groups, along with more than two methyl groups, improve the anti-wear performance of rapeseed oil. The application of data analysis and machine learning to investigate the classifications and structural characteristics of anti-wear additives in rapeseed oil provides data references and guiding principles for designing anti-wear additives in bio-based lubricants. Anti-wear performance is a crucial quality of lubricants, and it is important to conduct research into the structure-activity relationship of anti-wear additives in bio-based lubricants.
摘要：
Cardamine violifolia is a unique selenium hyperaccumulating vegetable in China, but its flowers are commonly wasted in large-scale cultivation. To better utilize this resource, this study explored the selenium content, chemical composition, and volatile organic compounds (VOCs) of hydro-distilling essential oil (EO) and hydrosol from C. violifolia flowers. ICP-MS results indicated that the EO and hydrosol contained selenium reaching 13.66±2.82 mg/kg and 0.0084±0.0013 mg/kg, respectively. GC-MS analysis revealed that organic acids, hydrocarbons, and amines were the main components of EO. Additionally, benzyl nitrile, benzaldehyde, benzyl isothiocyanate, benzyl alcohol, megastigmatrienone, and 2-methoxy-4-vinylphenol also existed in considerable amounts. The hydrosol extract had fewer components, mainly amines. HS-SPME-GC-MS corresponded to the composition analysis and aromatic compounds were the prevalent VOCs, while HS-GC-IMS primarily identified C2-C10 molecular alcohols, aldehydes, ethers, and sulfur-containing compounds. This study first described the chemical composition and VOC profiles of EO and hydrosol from selenium hyperaccumulating plant.
作者机构：
[Renchu Zhao; Dachang Chen; Jie Li; Qing Miao; Ke Liu] School of Electrical and Electronic Engineering, Wuhan Polytechnic University, Wuhan, China;[Beibei Xiao] School of Power Engineering, Jiangsu University of Science and Technology, Zhenjiang, China
摘要：
Equipment faults in power transformers can be detected by analyzing the dissolved gas in their internal insulation oil. In this study, the adsorption behavior and sensing properties of several characteristic dissolved gases (H2, CO, C2H2, and C2H4) on pristine and late transition metal (TM) (Fe, Co, Ni, and Cu)-doped MoTe2 were investigated using density functional theory (DFT) calculations. The adsorption energy, charge transfer ( ${Q}_{T}{)}$ , charge density difference (CDD), band structure, density of states (DOS), work function, and occupation function were compared to understand the gas adsorption behavior and electronic properties. The results demonstrate that TM-MoTe2 monolayers exhibit excellent sensing properties for H2, CO, C2H2, and C2H4 compared to pristine MoTe2. Among the four TM atoms, Co-doped MoTe2 shows the highest increase in adsorption energy for H2, CO, C2H2, and C2H4. The DOS analysis reveals clear hybridization between the molecular orbitals of the adsorbed gas molecules and the TM atomic orbitals for CO, C2H2, and C2H4 adsorption systems, indicating strong chemical interactions between these three gases and TM-MoTe2 surfaces. Moreover, the Co-MoTe2 surface exhibits superior sensitivity toward the adsorption of these four gas molecules. The introduction of TM doping shows a significant enhancement in adsorption selectivity, and TM-MoTe2 demonstrates the most dominant selective adsorption of CO molecules. This study provides valuable theoretical insights for the design of a new gas sensor aimed at detecting dissolved gases.
摘要：
BACKGROUND: Rosmarinic acid (RA), a polyphenol from edible-medical Lamiaceae herbs, is known to possess a variety of pharmacological activity, like anti-inflammatory, hepatoprotective and immunoregulation activities. METHODS AND RESULTS: Hereon, we investigated the anti-allergic activity of RA on immunoglobulin E (IgE)-mediated anaphylaxis responses in rat basophilic leukemia (RBL)-2H3 mast cell. RA hindered the morphological changes of IgE-induced degranulated RBL-2H3 cells. The release of two key biomarkers (β-hexosaminidase (β-HEX) and histamine) of IgE-induced degranulated mast cells was also remarkably down-regulated by RA intervention in a dose dependent manner. Moreover, RA inhibited IgE-induced ROS overproduction and flux of intracellular Ca(2+) in IgE-mediated degranulated mast cells. The q-PCR analysis showed that the expressions of genes (COX 2, PGD 2, LTC 4, HDC, Nrf2, HO-1 and NQO1) involved in MAPK and oxidative stress signaling pathways were significantly regulated by RA intervention. Moreover, the degranulation inhibitory effect of rosmarinic acid was investigated on the anti-DNP IgE/DNP-HSA induced passive cutaneous anaphylaxis (PCA) mice model in vivo. It showed that RA significantly inhibited the PCA reaction and allergic edema of ears in anti-DNP IgE/DNP-HSA stimulated mice. CONCLUSION: These findings suggest that RA has the potential to be used as a therapeutic candidate for allergic diseases by inhibiting mast cell degranulation. This indicates a possible role for RA in managing allergic reactions and related conditions.
摘要：
The aim of this study was to investigate the effects of dietary l-glutamine (Gln) supplementation on the morphology and function of the intestine and the growth of muscle in piglets. In this study, sixteen 21-day-old piglets were randomly divided into two groups: the Control group (fed a basal diet) and the Gln group (fed a basal diet supplemented with 0.81% Gln). Blood, gut, and muscle samples were collected from all piglets on Day 20 of the trial. Compared with the Control group, the supplementation of Gln increased (p < 0.05) the villus height, villus width, villus surface area, and villus height/crypt depth ratio of the small intestine. Furthermore, the supplementation of Gln increased (p < 0.05) total protein, total protein/DNA, and RNA/DNA in both the jejunum and ileum. It also increased (p < 0.05) the concentrations of carnosine and citrulline in the jejunal mucosa, as well as citrulline and cysteine concentrations in the ileum. Conversely, Gln supplementation decreased (p < 0.05) Gln concentrations in both the jejunum and ileum, along with β-aminoisobutyric acid and 1-Methylhistidine concentrations, specifically in the ileum. Subsequent research revealed that Gln supplementation increased (p < 0.05) the mRNA levels for glutathione-S-transferase omega 2 and interferon-β in the duodenum. In addition, Gln supplementation led to an increase (p < 0.05) in the number of Lactobacillus genus in the colon, but a decrease (p < 0.05) in the level of HSP70 in the jejunum and the activity of diamine oxidase in plasma. Also, Gln supplementation reduced (p < 0.05) the mRNA levels of glutathione-S-transferase omega 2 and interferon stimulated genes, such as MX1, OAS1, IFIT1, IFIT2, IFIT3, and IFIT5 in both the jejunum and ileum, and the numbers of Clostridium coccoides, Enterococcus genus, and Enterobacterium family in the colon. Moreover, Gln supplementation enhanced (p < 0.05) the concentrations of total protein, RNA/DNA, and total protein/DNA ratio in the longissimus dorsi muscle, the concentrations of citrulline, ornithine, arginine, and hydroxyproline, and the mRNA level of peptide transporter 1, while reducing the contents of hydrogen peroxide and malondialdehyde and the mRNA level of glutathione-S-transferase omega 2 in the longissimus dorsi muscle. In conclusion, dietary Gln supplementation can improve the intestinal function of piglets and promote the growth of the longissimus dorsi muscle.
摘要：
Plant autotoxicity is considered to be one of the important causes of continuous cropping obstacles in modern agriculture, which accumulates a lot of allelochemicals and xenobiotics and is difficult to solve effectively. To overcome tobacco continuous obstacles, a strain Pigmentiphaga kullae CHJ604 isolated from the environment can effectively degrade these compounds in this study. CHJ604 strain can degrade 11 types of autotoxicity allelochemicals and xenobiotics (1646.22μg/kg) accumulated in the soil of ten-years continuous cropping of tobacco. The 11 allelochemicals and xenobiotics significantly reduced Germination Percentage (GP), Germination Index (GI), and Mean Germination Time (MGT) of tobacco seeds, and inhibited the development of leaves, stems, and roots. These negative disturbances can be eliminated by CHJ604 strain. The degradation pathways of 11 allelochemicals and xenobiotics were obtained by whole genome sequence and annotation of CHJ604 strain. The heterologous expression of a terephthalate 1,2-dioxygenase can catalyze 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 4-hydroxybenzaldehyde, and 4-hydroxy-3-methoxy-benzaldehyde, respectively. The phthalate 4,5-dioxygenase can catalyze phthalic acid, diisobutyl phthalate, and dibutyl phthalate. These two enzymes are conducive to the simultaneous degradation of multiple allelochemicals and xenobiotics by strain CHJ604. This study provides new insights into the biodegradation of autotoxicity allelochemicals and xenobiotics as it is the first to describe a degrading bacterium of 11 types of allelochemicals and xenobiotics and their great potential in improving tobacco continuous obstacles.
摘要：
The use of microalgae-bacteria consortia (MBC) for wastewater treatment has garnered attention as their interactions impart greater environmental adaptability and stability compared with that obtained by only microalgae or bacteria use, thereby improving the efficiency of pollutant removal and bio-product productivity. Additionally, the value-added bio-products produced via biorefineries can improve economic competitiveness and environmental sustainability. Therefore, this review focuses on the interaction between microalgae and bacteria that leads to nutrient exchange, gene transfer and signal transduction to comprehensively understand the interaction mechanisms underlying their strong adaptability. In addition, it includes recent research in which MBC has been efficiently used to treat various wastewater. Moreover, the review summarizes the use of MBC-produced biomass in a biorefining context to produce biofuel, biomaterial, high-value bio-products and bio-fertilizer. Overall, more effort is needed to identify the symbiotic mechanism in MBC to provide a foundation for circular bio-economy and environmentally friendly development programmes.
摘要：
Although it is widely accepted that LTBI transitioning to ATB results from a disruption of host immune balance and a weakening of protective immune responses, the exact underlying immunological mechanisms that promote this conversion are not well characterized. This review systematically explores the immunoregulatory mechanisms in LTBI and ATB, along with their practical applications. Abstract Nearly one‐fourth of the global population is infected by Mycobacterium tuberculosis (Mtb), and approximately 90%–95% remain asymptomatic as latent tuberculosis infection (LTBI), an estimated 5%–10% of those with latent infections will eventually progress to active tuberculosis (ATB). Although it is widely accepted that LTBI transitioning to ATB results from a disruption of host immune balance and a weakening of protective immune responses, the exact underlying immunological mechanisms that promote this conversion are not well characterized. Thus, it is difficult to accurately predict tuberculosis (TB) progression in advance, leaving the LTBI population as a significant threat to TB prevention and control. This article systematically explores three aspects related to the immunoregulatory mechanisms and translational research about LTBI: (1) the distinct immunocytological characteristics of LTBI and ATB, (2) LTBI diagnostic markers discovery related to host anti‐TB immunity and metabolic pathways, and (3) vaccine development focus on LTBI. This article is categorized under: Infectious Diseases > Molecular and Cellular Physiology Infectious Diseases > Genetics/Genomics/Epigenetics Immune System Diseases > Genetics/Genomics/Epigenetics
摘要：
Edge -dependent states in electronic and synthetic graphenes have stimulated extensive research interests in recent years. The orbital degree of freedom (ODOF), facilitating the understanding of exotic phenomena in condensed matter, supplies a powerful base to explore boundary topological properties, which has not yet been investigated extensively in classical -wave realms such as acoustic systems. Here, we experimentally demonstrate orbital -dependent acoustic twig edge states in multi-ODOF systems as degenerate -orthogonal p orbitals are introduced. We analyze and discuss the band structures of orbital strip supercell lattices with the twig and zigzag edges by using the tight -binding method (TBM) and full -wave simulation (FWS). We visualize fourfold zero -energy orbital twig edge states that form the complete flat band across the Brillouin zone, characterized by nontrivial topological windings. Moreover, we unravel the selective excitation of the orbital twig edge states in experiments. Our findings advance the field for understanding the interplay between graphene edge states and acoustic orbitals as well as broaden the potential application opportunities of Dirac materials such as trapping and sensing.
摘要：
To improve the emulsifying property of sodium caseinate (NaCas) as stabilizer of oil-in-water emulsions and encapsulation of bioactive compounds, three hexaglycerol mono-fatty acid esters were chosen as small molecular weight surfactants to complex with NaCas. Hexaglycerol monooleate (HGMO) was found to be the optimal surfactant and the optimal mass ratio was 1:1, through characterization of particle size, Zeta-potential, and turbidity. Fluorescence and FTIR spectra indicated that the hydrophobic interaction and hydrogen bond provided driving forces to the formation of stable complex. The complexation of HGMO to NaCas increased the surface hydrophobicity and decreased surface tension compared with NaCas, and strengthened the EAI and ESI. The NaCas-HGMO complex had good stabilization on rice bran oil-in-water emulsions, in a wide pH and ionic strength, and the forwarding Cur encapsulation in O/W emulsions dramatically reduced the degradation during storage at 4celcius. Therefore, the present NaCas-HGMO complex might be employed as an effective emulsifier to stable O/W emulsions that load lipophilic bioactives in functional foods or beverages.
通讯机构：
[Mu, D ; Wang, C ] B;Beijing Jiaotong Univ, Sch Econ & Management, Beijing, Peoples R China.;Beijing Univ Technol, Coll Econ & Management, Beijing, Peoples R China.
关键词：
EV LIBs;Geopolitical disruptions;Supply chain network;Vulnerability assessment;Disruption ripple effect
摘要：
In the rapidly expanding global electric vehicle lithium -ion battery supply chain network (EV LIB SCN), intricate intercontinental and interrelated connections render it susceptible to geopolitical disturbances. The complex supplier-buyer dynamics within this network facilitate the propagation of disruptions, complicating the identification and evaluation of the consequences of geopolitical disruptions. Existing recommendations, grounded in macro -level material flow data, offer limited guidance for EV LIB -associated enterprises in managing such disruptions. To address this gap, this study constructs a meso-level EV LIB SCN incorporating realistic supplier-buyer relationship data among relevant firms. A supply chain network vulnerability (SCNV) index has been designed to quantify the disruption impact. Furthermore, a geopolitical disruption diffusion (GDD) model has been developed to simulate the disruption propagation in two distinct scenarios, i.e., single -region blockade and inter -region blockade. This study broadens the scope of EV LIB SCN investigations from macro- to meso-level analyses, and the evaluation of both direct and indirect impacts of geopolitical disruptions enhances comprehension of geopolitical disruption risks in global supply chain networks.
作者机构：
[Qian, Shenxian; Chen, Zhenzhen; Chen, Can; Shi, Pengfei; Qian, SX] Westlake Univ, Affiliated Hangzhou Peoples Hosp 1, Sch Med, Dept Hematol, Hangzhou, Peoples R China.;[Qian, Shenxian; Chen, Can; Kang, Dandan; Qian, SX] Zhejiang Univ, Sch Med, Hangzhou, Peoples R China.;[Li, Yun; Li, Y] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Team Neonatal & Infant Dev Hlth & Nutr NDHN, Wuhan, Peoples R China.;[Li, Yun; Li, Y] Kindstar Global Precis Med Inst, Wuhan, Peoples R China.
通讯机构：
[Li, Y ; Qian, SX ] W;Westlake Univ, Affiliated Hangzhou Peoples Hosp 1, Sch Med, Dept Hematol, Hangzhou, Peoples R China.;Zhejiang Univ, Sch Med, Hangzhou, Peoples R China.;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Team Neonatal & Infant Dev Hlth & Nutr NDHN, Wuhan, Peoples R China.;Kindstar Global Precis Med Inst, Wuhan, Peoples R China.
关键词：
Biomarker;CD8 + T cells;DLBCL;DLD;KDM1A
摘要：
Since the discovery of copper induces cell death(cuprotosis) in 2022, it has been one of the biggest research hotspots. cuprotosis related genes (CRGs) has been demonstrated to be a potential therapeutic target for cancer, however, the molecular mechanism of CRGs in coronavirus disease 2019 (COVID-19) infected in DLBCL patients has not been reported yet. Therefore, our research objective is first to elucidate the mechanism and role of CRGs in COVID-19. Secondly, we conducted univariate and multivariate analysis and machine learning to screen for CRGs with common expression differences in COVID-19 and DLBCL. Finally, the functional role and immune mechanism of genes in DLBCL were confirmed through cell experiments and immune analysis. The research results show that CRGs play an important role in the occurrence and development of COVID-19. Univariate analysis and machine learning confirm that dihydrolipoamide dehydrogenase (DLD) is the common key gene of COVID-19 and DLBCL. Inhibiting the expression of DLD can significantly inhibit the cycle progression and promote cell apoptosis of DLBCL cells and can target positive regulation of Lysine-specific demethylase 1 (LSD1, also known as KDM1A) to inhibit the proliferation of DLBCL cells and promote cell apoptosis. The immune analysis results show that high-expression of DLD may reduce T cell-mediated anti-tumor immunity by regulating immune infiltration of CD8 + T cells and positively regulating immune checkpoints LAG3 and CD276. Reducing the expression of DLD can effectively enhance T cell-mediated anti-tumor immunity, thereby clearing cancer cells and preventing cancer growth. In conclusion, DLD may be a potential therapeutic target for COVID-19 infection in DLBCL patients. Our research provides a theoretical basis for improving the clinical treatment of COVID-19 infection in DLBCL.
