腐竹的机械化生产关键技术研究
作者：
李诗龙;丁文平;张永林;侯传亮
期刊：
农业工程学报 ,2007年23(10):224-228 ISSN：1002-6819
通讯作者：
Li, S.(long6710@whpu.edu.cn)
作者机构：
[李诗龙; 张永林; 侯传亮] 武汉工业学院机械工程系;[丁文平] 武汉工业学院食品科学与工程学院
通讯机构：
Department of Machinery Engineering, Wuhan Polytechnic University, China
关键词：
腐竹;腐皮;豆制品;腐竹自动成形机;中试
摘要：
结合传统的腐竹结膜成形工艺,研发了一种新型腐竹自动成形机,采用涂布特氟龙的玻璃纤维槽型绷带输送装置实现了腐竹的一次性连续结膜,进行了机械化加工腐竹的中试,并就豆浆的温度、浓度以及pH值等因素对腐竹一次性连续结膜成形的影响进行了研究.试验结果表明,腐竹机械化揭膜的操作工艺要求与手工静态浆池揭膜成形明显不同,其最佳操作工艺条件为:当豆浆深度为8 mm时,豆浆温度为90℃,浓度为11%,pH值为7.5左右,此时浆液结膜速度快,腐膜质量好,腐竹出品率高达68%.
语种：
中文
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基于管式模型的小麦面筋系统流变行为的解析
作者：
朱帆;徐广文;丁文平
期刊：
农业工程学报 ,2007年23(7):24-29 ISSN：1002-6819
通讯作者：
Zhu, F.(stupidbadboy@sina.com)
作者机构：
[丁文平; 朱帆; 徐广文] College of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
College of Food Science and Technology, Wuhan Polytechnic University, China
关键词：
小麦面筋;动态流变学;表观活化能;时间-温度叠加
摘要：
利用动态流变仪对8种小麦面筋系统的进行小形变试验,研究在振动模式下的频率扫描时,面筋系统流变学特性,从分子动力学的角度进行解析,并引入Pierre Gilles de Gennes等人的管式模型理论基础性地对面筋流变行为进行阐述.试验表明:面筋蛋白转变温度在52.6～58.5 ℃间;0.1～40 ℃间,频率扫描下,时间-温度叠加法算出20℃时表观活化能在4602.57～6511.16 J/mol间,不同样品间有较大的差异;Arrhenius模型和WLF模型在拟合面筋系统叠合曲线的效果上一致.通过SPSS分析相关性表明:在面筋蛋白转变温度下,固定频率的温度扫描的弹性模量和在不同温度下的频率扫描的弹性模量成显著的正相关;面筋蛋白的转变温度与表观活化能呈较显著的正相关.该试验结果为小麦面筋相关制品的开发提供了参考.
语种：
中文
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大米淀粉纯化工艺及其性质的研究
作者：
陈季旺;刘英;刘刚;王慧溪
期刊：
农业工程学报 ,2007年23(9):225-228 ISSN：1002-6819
通讯作者：
Chen, J.(jiwangchen@yahoo.com.cn)
作者机构：
[陈季旺; 刘英; 刘刚; 王慧溪] School of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China;Hubei Technology Research Centre of Rice Process Engineering, Wuhan 430023, China
通讯机构：
School of Food Science and Technology, Wuhan Polytechnic University, China
关键词：
大米淀粉;碱性蛋白酶;扫描电子显微镜(SEM);溶解度;膨润力
摘要：
该文采用碱性蛋白酶对碱法制备得到的大米淀粉进行纯化,酶解条件为 pH 9,温度45℃,酶与底物比48 AU/kg,时间60 min.大米淀粉中的蛋白质含量从2.87%降低到0.40%.扫描电镜分析大米淀粉的超微结构显示,碱性蛋白酶纯化处理后的大米淀粉中未见明显的蛋白质颗粒存在.比较纯化前后的大米淀粉发现,大米淀粉经过碱性蛋白酶纯化后,其溶解度和膨润力都明显增加.
语种：
中文
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Pretreatment of bamboo residues with Coriolus versicolor for enzymatic hydrolysis
作者：
Zhang, Xiaoyu* ;Xu, Chunyan;Wang, Hongxun
期刊：
Journal of Bioscience and Bioengineering ,2007年104(2):149-151 ISSN：1389-1723
通讯作者：
Zhang, Xiaoyu
作者机构：
[Zhang, Xiaoyu] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.;Wuhan Polytech Univ, Coll Food Sci & Technol, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, Xiaoyu] H;Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.
关键词：
white-rot fungi;biological treatment;fuel ethanol;bamboo
摘要：
Pretreatment by a white-rot fungus Coriolus versicolor B1 under different conditions and saccharification of bamboo were investigated. The saccharification rate was significantly enhanced and a maximum saccharification rate of 37.0% was achieved after pretreatment. Reducing sugars yield was 223.2 mg/g of bamboo residues, which was 2.34 times that of the raw material. It was feasible to treat bamboo residues with B1 for the saccharification of bamboo.
语种：
英文
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高水分稻谷组合清理机设计与试验研究
作者：
王继焕;刘启觉
期刊：
农业工程学报 ,2006年22(1):102-106 ISSN：1002-6819
通讯作者：
Wang, J.
作者机构：
[王继焕] 武汉工业学院机械工程系;[刘启觉] 武汉工业学院食品科学与工程系
通讯机构：
Mechanical Engineering Department, Wuhan Polytechnic University, China
关键词：
高水分稻谷;组合清理机;试验研究;纤维性杂质
摘要：
刚收割的高水分稻谷中夹杂较多碎禾叶、禾秆等纤维性杂质，进入烘干机之前必须进行清理。针对国内外对高水分稻谷清理技术研究很少和现有稻谷清理设备不适合处理高水分稻谷的现状，设计了稻谷组合清理机。运用生产试验和现场检测的方法，分析处理量与稻谷水分、设备吸风量、筛孔尺寸及分布、筛板倾角及分布、振动频率等参数之间的联系与相互作用。试验表明：当稻谷含水率高于20％时，组合清理机的筛孔尺寸按卜层50×50、中层30×30、下层15×15分布，筛板倾角按上层21°、中层17°、下层13°布置，并且穿过筛孔的实际风速为稻谷悬浮速度的1．1～1．2倍时，处理量较大，清理效果较好。
语种：
中文
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Rheological properties of low methoxyl pectin
作者：
汪海波;徐群英;汪芳安
期刊：
农业工程学报 ,2006年22(11):223-227 ISSN：1002-6819
通讯作者：
Wang, H.(wanghaibo@whpu.edu.cn)
作者机构：
[汪芳安; 汪海波; 徐群英] Department of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430022, China
通讯机构：
Department of Food Science and Engineering, Wuhan Polytechnic University, China
关键词：
低酯果胶;流变学性能;温度;剪切速率;pH值;金属离子;糖
摘要：
以商品低酯果胶为研究对象,以AR-500型动态流变仪为主要设备,考察低酯果胶溶液的流变学基本特性,以及温度、剪切速率、体系pH值、金属离子、糖等因素对其流体性能的影响.结果表明,低酯果胶溶液是典型的剪切稀化型非牛顿流体,果胶浓度越高,溶液剪切稀化现象越严重.同时其溶液流体行为受温度、剪切速率、pH值等因素的影响.体系温度和pH值的升高均可导致溶液黏度降低;剪切速率的影响与果胶浓度有关,在低浓度条件下(0.3%),低速率时(0～200 s~(-1))剪切稀化现象越严重,高速率(400～700 s~(-1))时转变为胀塑性流体;在高浓度(1%、2%)条件下,随着剪切速率增高,溶液流体逐渐趋向于理想状态的牛顿流体.金属二价离子对低酯果胶溶液有显著的增稠效应,其增稠效果与离子浓度呈正相关.
语种：
中文
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大米肽的酶法制备工艺及其特性的研究
作者：
陈季旺;孙庆杰;夏文水;汪志华;章焦
期刊：
农业工程学报 ,2006年22(6):178-181 ISSN：1002-6819
通讯作者：
Chen, J.
作者机构：
[陈季旺; 夏文水; 汪志华] 武汉工业学院食品科学与工程学院;[孙庆杰] 湖南金健米业股份有限公司;[章焦] 东南香米业发展有限公司
通讯机构：
School of Food Science and Engineering, Wuhan Polytechnic University, China
关键词：
大米肽;碱性蛋白酶;工艺;特性
摘要：
该文利用蛋白酶水解大米蛋白制备得到大米肽.比较碱性蛋白酶、中性蛋白酶、复合蛋白酶和风味酶水解大米蛋白的进程曲线,结果显示碱性蛋白酶的水解效果最好,其较佳作用条件为:底物浓度10%、pH值9.0、温度45℃、酶与底物比48 AU/kg、时间150 min.在此条件下,大米肽的得率为46.8%,纯度为71.3%.大米肽具有溶解性较好和黏度较低的特性,可以在食品中广泛应用.
语种：
中文
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A New Process for Preparation of Soybean Protein Concentrate with Hexane-Aqueous Ethanol Mixed Solvents
作者：
Zhang, WN* ;Liu, DC
期刊：
JOURNAL OF AOAC INTERNATIONAL ,2005年88(4):1217-1222 ISSN：1060-3271
通讯作者：
Zhang, WN
作者机构：
[Liu, DC; Zhang, WN] Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, WN] W;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
摘要：
A new process for the preparation of soybean protein concentrate (SPC) by directly extracting full-fat soy flour with a mixture of hexane and aqueous ethanol was established. Compared with conventional methods, it has some advantages, such as saving energy and reducing protein denaturation caused by heat action during solvent recovery, because this process saves one step of solvent recovery. The effects of aqueous ethanol concentration and the mixure ratio (hexane to ethanol) on the degree of protein denaturation and product quality were investigated, on the basis of which the orthogonal tests were performed. The optimum technical parameters were obtained by analyzing the results of the orthogonal tests with statistical methods. We found that SPC can be obtained by extracting full-fat soy flour under the following conditions: mixture ratio hexane: 90% ethanol, 9:1, v/v; extraction temperature, 45°C; ratio of solid to solvents, (1:2 w/v); and 5 repeated extractions (15 min each time). The results of quality analysis showed that solubility of the product was improved significantly [nitrogen solubility index (NSI) 46.6%] compared with that for ethanol washing of protein concentrate (NSI 8.7%).
语种：
英文
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High-performance liquid chromatography of some basic drugs on a n-octadecylphosphonic acid modified magnesia-zirconia stationary phase
作者：
He, HB;Feng, YQ* ;Da, SL;Hu, ZX;Qu, L
期刊：
JOURNAL OF SEPARATION SCIENCE ,2005年28(13):1577-1586 ISSN：1615-9306
通讯作者：
Feng, YQ
作者机构：
[Feng, YQ] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan, Peoples R China.
通讯机构：
[Feng, YQ] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
high-performance liquid chromatography;n-octaclecylphosphonic acid modification;magnesia-zirconia;basic drugs
摘要：
The high-performance liquid chromatographic behavior of some basic drugs was studied on a n-octadecylphosphonic acid modified magnesia-zirconia (C<inf>18</inf>PZM) stationary phase. The effect of mobile phase variables such as methanol content, ionic strength, and pH on their chromatographic behavior was investigated. The retention mechanism of basic drugs on the stationary phase was elucidated. The results indicate that both hydrophobic and cation-exchange interactions contribute to solute retention under most chromatographic conditions. The inherent Bro¨nsted-acid sites and also the adsorbed Lewis base anionic buffer constituents on accessible ZM surface Lewis acid sites play a role in the retention of ionized solutes by cation-exchange interaction. However, especially at high mobile phase pH, the retention of basic drugs depends mainly on hydrophobic interactions between solutes and support. Separations of the basic drugs on the C<inf>18</inf>PZM phase by a predominantly reversed-phase retention mode were very promising. The mixed-mode retention feature on this phase, as a result of the adsorbed Lewis base anionic buffer constituents acting as sites for cation-exchange, could also be very useful, e.g. for enhancing the chromatographic selectivity of such analytes. The C<inf>18</inf>PZM seems to be an excellent alternative to silica-based reversed-phase stationary phase for the separation of strongly basic solutes. ©2005 Wiley-VCH Verlag GmbH &Co. KGaA, Weinheim.
语种：
英文
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高水分稻谷干燥工艺试验研究
作者：
刘启觉
期刊：
农业工程学报 ,2005年21(2):135-139 ISSN：1002-6819
通讯作者：
Liu, Q.
作者机构：
[刘启觉] Department of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
Department of Food Science and Engineering, Wuhan Polytechnic University, China
关键词：
变温干燥;稻谷裂纹;降水速率;缓苏烘干比
摘要：
针对中国南方地区稻谷收获季节需及时干燥高水分稻谷的市场要求,采用试验方法,在分批循环式稻谷干燥机上试验了低恒温干燥、变温干燥和变温干燥过程中增加缓苏时间的三种干燥工艺.依据试验结果,分析稻谷含水率、干燥介质温度、稻谷温度、缓苏烘干时间比等参数之间的联系与相互作用.试验表明:稻谷含水率高于21%时,降水速率可大于每小时1%,可采用60～70℃的介质.当稻谷含水率小于18%时,介质温度应小于60℃,降水速率小于每小时1%.当高水分稻谷进行了3～4次烘干缓苏后,利用中间缓苏仓增加缓苏时间,使稻谷内部与表层的温度、水分趋于平衡,有利于改善烘后品质和后续工艺的干燥降水.该结论对高水分稻谷干燥工艺设计和设备研制具有实用参考价值.
语种：
中文
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Study on interaction between drug and membrane by using liposome coated zirconia–magnesia chromatography
作者：
Zhang, WN;Hu, ZX;Liu, Y;Feng, YQ* ;Da, SL
期刊：
Talanta ,2005年67(5):1023-1028 ISSN：0039-9140
通讯作者：
Feng, YQ
作者机构：
[Feng, YQ] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Feng, YQ] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
liposome chromatography;drug absorption;zirconia;phosphatidylcholine
摘要：
The interactions between drug molecules and membrane were studied using the new chromatography stationary phase of liposome coated zirconia-magnesia. logK(s)(ZrO(2)-MgO) on this new chromatography for some drugs, compared with that on liposome coated silica chromatography and other reported data, fair correlations were observed between them when excluding effect of special adsorption. logK(s)(ZrO(2)-MgO) values for barbitalum, diazepam, benzene, benzocaine and toluene correlated well with corresponding values on liposome coated silica chromatography (R=0.99778, P<0.001; R=0.98229, P<0.003; R=0.9985, P<0.0001; R=0.99925, P<0.0001, pH value of mobile phase at pH 7.4, 7.0, 6.4 and 5.4, respectively). They also correlated well with the literature data on immobilized artificial membrane chromatography (R=0.99999, P<0.004 at pH 7.4) and liposome chromatography (R=0.99994, P<0.008) for procaine, lidocaine and bupivacaine. Liposome coated zirconia-magnesia chromatography can thus be used for studying drug-membrane interaction and prediction of drug absorption as another liposome chromatography method.
语种：
英文
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Adsorptive voltammetry of Hg(II) ions at a glassy carbon electrode coated with electropolymerized methyl-red film
作者：
Yang, NJ;Wan, QJ* ;Yu, JH
期刊：
Sensors and Actuators B-Chemical ,2005年110(2):246-251 ISSN：0925-4005
通讯作者：
Wan, QJ
作者机构：
[Wan, QJ] Wuhan Inst Technol, Sch Chem Engn & Pharm, Hubei Key Lab Novel Reactor & Green Chem Technol, Wuhan 430073, Peoples R China.;Univ Fukui, Grad Sch Engn, Fukui 9108507, Japan.;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.
通讯机构：
[Wan, QJ] W;Wuhan Inst Technol, Sch Chem Engn & Pharm, Hubei Key Lab Novel Reactor & Green Chem Technol, Wuhan 430073, Peoples R China.
关键词：
modified electrode;adsorptive voltammetry;electropolymerization;methyl red;Hg(II) ion;ANODIC-STRIPPING VOLTAMMETRY;MODIFIED SOLID ELECTRODES;PASTE ELECTRODES;GOLD ELECTRODE;DISK ELECTRODE;MERCURY;POLYPYRROLE;PRECONCENTRATION;ACID
摘要：
The glassy carbon electrode coated with electropolymerized methyl-red film, 1.2 x 10(-6) m in thickness, (PMRE) showed high sensitivity towards Hg(II) ions. PMREs were adopted to accumulate and detect H(II) ions in a pH 2.56 Britton-Robinson buffer solution. Cyclic voltammogram of the accumulated Hg species on PMREs exhibited an anodic wave at 0.64 V and a cathodic wave at 0.13 V, due to the oxidation of accumulated Hg species on PMREs and the reduction of Hg(II) ions in the solution, respectively. For this heterogeneous adsorption of Hg(11) ions onto PMREs, the maximum surface concentration, adsorption equilibrium, and Gibbs energy change were evaluated to be 5.12 x 10(-6) mol m(-2), 3.7 x 10(5) l mol(-1), and -30.1 kJ mol(-1), respectively. The anodic peak current at 0.64 V was linear with the concentration of Hg(H) ions in the range of 1.1 x 10(-10) to 1.1 x 10(-7) M with a detection limit of 4.4 x 10(-11) M. The proposed method was utilized successfully for the detection of Hg(II) ions in the lake water. (C) 2005 Elsevier B.V. All rights reserved.
语种：
英文
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Magnesia-zirconia based mimetic biomembrane chromatography for predicting human drug absorption
作者：
Hu, ZX;Zhang, WN;He, HB;Feng, YQ;Da, SL*
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2005年827(2):173-181 ISSN：1570-0232
通讯作者：
Da, SL
作者机构：
[Da, SL] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Da, SL] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
Magnesia-zirconia composite;Mimetic biomembrane stationary phase;Oral drug absorption
摘要：
In this paper, a novel mimetic biomembrane chromatography stationary phase of magnesia-zirconia composite matrix were prepared with the Lewis acid-base interaction between phosphatidylcholine's residue phosphonate group and Lewis acid sites of magnesia-zirconia composite;the retention factors of a chemically diverse set of drugs on the new stationary phase were determined;the drugs log K<inf>mbm</inf> values were correlationed with the absorbed fraction of drugs orally administered in humans (%F<inf>a</inf>) and a hyperbolic relationship was obtained. Meanwhile, the relationship between the log K<inf>mbm</inf> values and hydrophobic parameters (log P<inf>oct</inf> and log D<inf>oct</inf>) were discussed. The usefulness of the new column for predicting oral drug absorption in humans is demonstrated by comparing this model with IAM, ILC and BMC models. Results show that the log K<inf>mbm</inf> values have good relationship with logKWIAM, log K<inf>BMC</inf> and have moderate to fair relationship with log K<inf>s</inf> determined on four different ILC column (EPL, PC, PC-PE, PC-PS). Therefore, the log K<inf>mbm</inf> values can provide key information about the transport properties of drugs and this chromatographic model may be applicable for prediction of drug uptake through epithelial cell membranes during the drug discovery process. ©2005 Elsevier B.V. All rights reserved.
语种：
英文
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Optimization of parameters of high-performance displacement chromatography for separation of soybean phosphatidylcholine and phosphatidylethanolamine
作者：
Zhang, WN;Hu, ZX;Feng, YQ;Da, SL*
期刊：
Journal of Chromatography A ,2005年1068(2):269-278 ISSN：0021-9673
通讯作者：
Da, SL
作者机构：
[Da, SL] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Da, SL] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
displacement chromatography;phosphatidylcholine;phosphatidylethanolamine;soybean phospholipids;lipids
摘要：
Hundred milligrams of soybean phospholipids were successfully separated by using high-performance displacement chromatography (HPDC) on a 150 mm x 4.6 mm analytical silica column (3-5 μm packings) with dichloromethane-methanol (9:1, v/v) as carrier and ethanolamine as displacer. From the viewpoint of preparative separation, the effects of loading amount, concentration and flow-rate of displacer on separation efficiency were investigated using throughput and recovery as indices. The parameters were optimized by orthogonal test design and statistical analysis method. Under the optimum conditions, namely displacer concentration being 167 mM, the flow-rate of displacer at 0.2 ml/min and concentration of sample being 211 mg/ml (factual loading amount 211 mg/ml x 0.7 ml = 148 mg), the purity, throughput and recovery of obtained soybean phosphatidylethanolamine (PE) and phosphatidylcholine (PC) were 80.2%, 65.7 mg/h, 70.9% and 90.5%, 272.6 mg/h, 88.3%, respectively. In addition, selections of regenerant and appropriate regeneration condition were also studied. ©2005 Elsevier B.V. All rights reserved.
语种：
英文
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Preparation and evaluation of n-octadecylphosphonic acid-modified magnesia–zirconia stationary phases for reversed-phase liquid chromatography
作者：
He, HB;Feng, YQ* ;Qu-Li;Da, SL;Hu, ZX
期刊：
Analytica Chimica Acta ,2005年542(2):268-279 ISSN：0003-2670
通讯作者：
Feng, YQ
作者机构：
[Feng, YQ] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430072, Peoples R China.
通讯机构：
[Feng, YQ] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
Alkaloids;Basic compounds;Magnesia-zirconia;Octadecylphosphonic acid-modified;Reversed-phase liquid chromatography;Stationary phases
摘要：
Three n-octadecylphosphonic acid-modified magnesia-zirconia reversed stationary phases (C18PZM) are prepared via the strong Lewis base interactions between organophosphonate and magnesia-zirconia composite. And two of them are end-capped by using trimethylchlorosilane as end-capping agent in different procedures. Stability studies at extreme high pH conditions (pH 9-12) show that both the non-endcapped and endcapped columns are quite stable at pH 12 mobile phase. The reversed-phase liquid chromatographic behavior of three C18PZM stationary phases are comparatively investigated in detail using a variety of basic compounds as probes. The retention of basic compounds on the three phases is studied over a wide range of pHs. And the possible retention mechanisms of basic compounds on the three stationary phases are discussed. The results show that the basic solutes retain by a hydrophobic and cation-exchange interaction mixed mechanism on three stationary phases when they are operated in eluents at pH values near to the pKa of the Brönsted conjugate acid form of the analyte, suggesting that inherent zirconol groups on ZM are not expected to interact with bases via cation-exchange interaction at lower pH. Nonetheless, the non-endcapped phase differs markedly from the edncapped ones in retention and selectivity of basic solutes using eluents at pH 4.1, implying a complex retention mechanism at this pH. The cation-exchange sites under such conditions are more likely due to the adsorbed Lewis base anionic buffer constituents (acetate) on accessible ZM surface sites than the chemisorbed phosphonate. Although the three phases exhibit very similar chromatographic behavior with eluents at pH 10.1, and show in general satisfactory separation of basic compounds and alkaloids studied, the performance for a specific analyte, however, differs largely from column to column. © 2005 Elsevier B.V. All rights reserved.
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Preparation and characterization of a magnesia–zirconia stationary phase modified with β-cyclodextrin for reversed-phase high-performance liquid chromatography
作者：
He, HB;Zhang, WN;Da, SL;Feng, YQ*
期刊：
Analytica Chimica Acta ,2004年513(2):481-492 ISSN：0003-2670
通讯作者：
Feng, YQ
作者机构：
[Feng, YQ] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Feng, YQ] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
β-Cyclodextrin;Alkylbenzenes;HPLC;Magnesia-zirconia composite;Positional isomers
摘要：
A new stationary phase of magnesia-zirconia composite matrix for high-performance liquid chromatography was first prepared by modification of β-cyclodextrin (β-CD) via fosfomycin as a spacer. Various modification procedures were attempted for achievement of successful modification. The modified composite was characterized by using coloration, elemental analysis, diffused reflectance FT-IR, surface area and pore size distribution. The separation of alkylbenzenes, polycyclic aromatic hydrocarbons, positional isomers of some acidic, basic and amphoteric disubstituted benzenes was studied on the new stationary phase. The effect of pH and methanol content in the mobile phase on retention and separation selectivity for the positional isomers were investigated. The chromatographic performance of CD modified magnesia-zirconia was compared with fosfomycin modified magnesia-zirconia as intermediate material and bare magnesia-zirconia as raw material. The results show that various retention mechanisms such as hydrophobicity, inclusion complexation and hydrogen bond interaction exist in the chromatography process of the packing modified with CD. The β-CD played the major role in the chromatographic property of this new stationary phase. The modified magnesia-zirconia exhibits superiority of separation for basic aromatics and high stability above pH 11. © 2004 Elsevier B.V. All rights reserved.
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英文
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Separation of phosphatidylcholine and phosphatidylethanolamine by using high-performance displacement chromatography
作者：
Zhang, WN;He, HB;Feng, YQ;Da, SL*
期刊：
Journal of Chromatography A ,2004年1036(2):145-154 ISSN：0021-9673
通讯作者：
Da, SL
作者机构：
[Da, SL] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Da, SL] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
Displacement chromatography;Phosphatidylcholine;Phosphatidylethanolamine;Phospholipids;Lipids
摘要：
A binary mixture of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was successfully separated by high-performance displacement chromatography (HPDC) on an 150 mm x 4.6 turn analytical silica column (3-5 mum packing), using dichloromethane-methanol (9: 1, v/v) as carrier and ethanolamine as displacer. The effects of displacer concentration, flow-rate, loading amount and the composition of the sample on separation efficiency were studied. Eighty-four milligrams sample (PE:PC 1:1.16) was separated perfectly by using 83 mM ethanolamine (in carrier) as displacer at the flow-rate of 0.1 ml/min. The yields of the pure PE and PC (100% purity) were 94.8% and 87.9%, respectively and the cycle time for a single separation was about 195 min. It was valuable that the optimum loading amount (the allowed maximum of sample loading) was investigated only by using the sample to be simulated the composition of the separated actual one, because the separation efficiency was significantly affected by the composition of the sample. For the same loading amount of 175 mg, the yields of the pure PE and PC were improved greatly from 31.4 and 16.9 to 56.0 and 77.6%, respectively, when the proportion of PE to PC was adjusted from 1:1.16 to 1:4. Furthermore, the separation of PE and PC in an actual sample (soybean phospholipids) was achieved using the proposed HPDC method. (C) 2004 Elsevier B.V. All rights reserved.
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Separation and purification of phosphatidylcholine and phosphatidylethanolamine from soybean degummed oil residues by using solvent extraction and column chromatography
作者：
Zhang, WN;He, HB;Feng, YQ;Da, SL*
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2003年798(2):323-331 ISSN：1570-0232
通讯作者：
Da, SL
作者机构：
[Da, SL] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Da, SL] W;Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China.
关键词：
Phosphatidylcholine;Phosphatidylethanolamine
摘要：
Natural phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were separated and purified from soybean degummed oil residues in this work. Crude PC and PE were first separated from degummed oil residues by extraction with 95% ethanol, and then the crude PC and PE were used as raw materials to prepare high purity PC and PE by using column chromatography of silica gel (100-200 mesh) with different eluents and elution modes. The high purity PC (content >90%) was obtained from the crude PC by using isocratic elution with methanol as eluent. Compared with the methods reported by using isocratic elution with mixed solvents as eluent or gradient elution, the procedure proposed exhibits low cost and industry potentialities because of some advantages, such as operation simplicity, cheap equipment and solvent to be recovered easily. The purity of the PE product prepared from the crude PE was more than 75%. The gradient elution was preferable to isocratic elution for reducing the elution time and eluent consumption when to prepare PE from the crude PE. The effects of loading amount and the flow-rate on separation efficiency were also investigated. For obtaining high separation efficiency, the loading amount should be less than 2.0g crude PC or PE/100g silica gel, and the flow-rate should be controlled under 4ml/min for crude PC and 3ml/min for crude PE, respectively. ©2003 Elsevier B.V. All rights reserved.
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