期刊：
International Journal of Biological Macromolecules,2025年308(Pt 3):142352 ISSN：0141-8130
通讯作者：
Cai, J
作者机构：
[Cai, Jie; Liu, Nian; Yang, Zhaoxing; He, Zhijun; Feng, Xiaofang; Xie, Fang] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Liu, Xiaoqing] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Delivery;OSA starch;Selenium nanoparticles
摘要：
Selenium nanoparticles (SeNPs) exhibit significant potential in antitumor therapy. However, challenges such as aggregation and lack of targeting capability limit their application. Herein, we developed selenium-loaded octenyl succinic anhydride starch (OSAS) micelles functionalized with folic acid (FA) for targeted tumor delivery. The FA-OSAS-SeNPs were synthesized through self-assembly, incorporating SeNPs into FA-conjugated OSAS micelles. Fourier Transform Infrared (FTIR) spectroscopy and UV–visible spectrophotometry confirmed the successful synthesis of FA-OSAS-SeNPs. The nanoparticles exhibited an average size of 131.66 ± 7.88 nm and a zeta potential of −19.54 ± 0.33 mV, with encapsulation efficiency and drug loading capacity of approximately 87.28 % and 8.96 %, respectively. FA-OSAS-SeNPs demonstrated good stability across various conditions, including different dilution ratios, temperatures, pH levels, and ionic strengths. In vitro studies showed that FA-OSAS-SeNPs exhibited significant targeted inhibitory effects on cervical cancer (HeLa) cells and markedly increased intracellular ROS levels, inducing apoptosis. This study presents a novel and effective strategy for targeted SeNPs delivery systems in tumor therapy, offering a valuable reference for future development of nanomaterials for clinical applications in cancer treatment.
Selenium nanoparticles (SeNPs) exhibit significant potential in antitumor therapy. However, challenges such as aggregation and lack of targeting capability limit their application. Herein, we developed selenium-loaded octenyl succinic anhydride starch (OSAS) micelles functionalized with folic acid (FA) for targeted tumor delivery. The FA-OSAS-SeNPs were synthesized through self-assembly, incorporating SeNPs into FA-conjugated OSAS micelles. Fourier Transform Infrared (FTIR) spectroscopy and UV–visible spectrophotometry confirmed the successful synthesis of FA-OSAS-SeNPs. The nanoparticles exhibited an average size of 131.66 ± 7.88 nm and a zeta potential of −19.54 ± 0.33 mV, with encapsulation efficiency and drug loading capacity of approximately 87.28 % and 8.96 %, respectively. FA-OSAS-SeNPs demonstrated good stability across various conditions, including different dilution ratios, temperatures, pH levels, and ionic strengths. In vitro studies showed that FA-OSAS-SeNPs exhibited significant targeted inhibitory effects on cervical cancer (HeLa) cells and markedly increased intracellular ROS levels, inducing apoptosis. This study presents a novel and effective strategy for targeted SeNPs delivery systems in tumor therapy, offering a valuable reference for future development of nanomaterials for clinical applications in cancer treatment.
摘要：
A novel peptide attenuating skeletal muscle atrophy was prepared, identified, screened from corn and its molecular mechanism was explored using two-step enzymatic hydrolysis, molecular docking, and sarcopenia mice model. The results showed that the DPPH free radical scavenging rate of corn peptides (CPs) was 45.20 % under the optimum preparation conditions. Fifty-one peptide fragments were identified from CPs, among which QQPIVGGA, QYQLPSY, LQQQLL, and LQQQQL presented superior affinity with mTORC1 and FOXO in molecular docking. LQQQLL (0.02 mM) significantly increased the proliferative activity of senescent C2C12 cells by 41.67 % compared with the model group ( P < 0.05), showing the potential to attenuate skeletal muscle atrophy. The sarcopenia mice model results indicated that CPs and LQQQLL significantly improved the content of total superoxide dismutase (T-SOD), skeletal muscle mass index (SMI), and decreased the level of malondialdehyde (MDA), tumor necrosis factor (TNF)-α, muscle atrophy protein Fbox-1 (Atrogin-1), and 8-hydroxydeoxyguanosine (8-OHdG) ( P < 0.05). CPs and LQQQLL also markedly increased the cross-sectional muscle area and the relative content of type II muscle fibers in sarcopenia mice. Additionally, CPs and LQQQLL significantly up-regulated the expression levels of PI3K, AKT and mTOR proteins ( P < 0.05), reduced the proliferation of Proteobacteria , Actinobacteriota , Desulfobacterota, and Staphylococcus and promoted the proliferation of Bacteroidota, and Lactobacillus . In conclusion, CPs and LQQQLL could activate the PI3K/AKT/mTOR signaling pathway, and reduce the proliferation of pathogens and promote the proliferation of intestinal microorganisms, thus improving the attenuation of skeletal muscle.
A novel peptide attenuating skeletal muscle atrophy was prepared, identified, screened from corn and its molecular mechanism was explored using two-step enzymatic hydrolysis, molecular docking, and sarcopenia mice model. The results showed that the DPPH free radical scavenging rate of corn peptides (CPs) was 45.20 % under the optimum preparation conditions. Fifty-one peptide fragments were identified from CPs, among which QQPIVGGA, QYQLPSY, LQQQLL, and LQQQQL presented superior affinity with mTORC1 and FOXO in molecular docking. LQQQLL (0.02 mM) significantly increased the proliferative activity of senescent C2C12 cells by 41.67 % compared with the model group ( P < 0.05), showing the potential to attenuate skeletal muscle atrophy. The sarcopenia mice model results indicated that CPs and LQQQLL significantly improved the content of total superoxide dismutase (T-SOD), skeletal muscle mass index (SMI), and decreased the level of malondialdehyde (MDA), tumor necrosis factor (TNF)-α, muscle atrophy protein Fbox-1 (Atrogin-1), and 8-hydroxydeoxyguanosine (8-OHdG) ( P < 0.05). CPs and LQQQLL also markedly increased the cross-sectional muscle area and the relative content of type II muscle fibers in sarcopenia mice. Additionally, CPs and LQQQLL significantly up-regulated the expression levels of PI3K, AKT and mTOR proteins ( P < 0.05), reduced the proliferation of Proteobacteria , Actinobacteriota , Desulfobacterota, and Staphylococcus and promoted the proliferation of Bacteroidota, and Lactobacillus . In conclusion, CPs and LQQQLL could activate the PI3K/AKT/mTOR signaling pathway, and reduce the proliferation of pathogens and promote the proliferation of intestinal microorganisms, thus improving the attenuation of skeletal muscle.
摘要：
Lotus root is a widely popular aquatic vegetable with edible and medicinal values. Here we report the structure and lipid-lowering activity of two lotus root polysaccharides LRW (lotus root polysaccharide by water extraction) and LRA (lotus root polysaccharide by alkali extraction), that were extracted by aqueous and alkaline solution respectively. The results showed that the yield of polysaccharide from lotus root could be significantly improved by alkali extraction. Basic composition and structural characterization showed that the total sugar contents of LRW and LRA were 96.83 % and 73.66 %, and the molecular weights were 2.464 × 10 5 Da and 1.727 × 10 5 Da, respectively. LRW and LRA had the similar structure that the main backbone consisted of →4)-α-D-Glcp-(1→ with branches at C-6 site. Both LRW and LRA could scavenge DPPH and hydroxyl radicals effectively, and have strong adsorption capacity to cholate salts in a concentration-dependent manner. In HepG2 cells, LRW and LRA inhibited the accumulation of lipid droplets induced by oleic acid, and increased the activity of T-SOD and CAT, meanwhile, reduced the level of MDA, TC and TG, showing good lipid-lowering activity. In comparison, the lipid-lowering effect of LRA was better than that of LRW. In addition, gene sequencing and RT-PCR showed that AMPK, ACC, PPARα and CPT-1 were essential for LRA to exert a lipid-lowering effect. This study provides a theoretical basis for the extraction and lipid-lowering application of lotus root polysaccharides.
Lotus root is a widely popular aquatic vegetable with edible and medicinal values. Here we report the structure and lipid-lowering activity of two lotus root polysaccharides LRW (lotus root polysaccharide by water extraction) and LRA (lotus root polysaccharide by alkali extraction), that were extracted by aqueous and alkaline solution respectively. The results showed that the yield of polysaccharide from lotus root could be significantly improved by alkali extraction. Basic composition and structural characterization showed that the total sugar contents of LRW and LRA were 96.83 % and 73.66 %, and the molecular weights were 2.464 × 10 5 Da and 1.727 × 10 5 Da, respectively. LRW and LRA had the similar structure that the main backbone consisted of →4)-α-D-Glcp-(1→ with branches at C-6 site. Both LRW and LRA could scavenge DPPH and hydroxyl radicals effectively, and have strong adsorption capacity to cholate salts in a concentration-dependent manner. In HepG2 cells, LRW and LRA inhibited the accumulation of lipid droplets induced by oleic acid, and increased the activity of T-SOD and CAT, meanwhile, reduced the level of MDA, TC and TG, showing good lipid-lowering activity. In comparison, the lipid-lowering effect of LRA was better than that of LRW. In addition, gene sequencing and RT-PCR showed that AMPK, ACC, PPARα and CPT-1 were essential for LRA to exert a lipid-lowering effect. This study provides a theoretical basis for the extraction and lipid-lowering application of lotus root polysaccharides.
摘要：
The effect of Chlorella pyrenoidosa (CP) and Spirulina platensis (SP) at concentrations of 0 %–12 % on the properties of rice starch (RS) was investigated. Compared with pure RS, the addition of CP and SP powder decreased the viscosity, increased the gelatinization temperature, and promoted the retrogradation of RS gel. However, when CP was added at 12 % and SP at 8 %, retrogradation inhibition was reduced. At these concentrations, the relative crystallinity of the CP mixture increased by 57.37 %, whereas that of SP increased by 48.13 %. Scanning electron microscopy revealed that the addition of low amount of CP and SP reduced porosity. CP and SP powder facilitated the conversion of bound water to free water and contributed to the weakening of the viscoelasticity of the RS gel. CP powder likely had a more detrimental effect on the short-term storage properties of RS than SP powder. These results provide theoretical support for the development of RS-based products and the innovative utilization of microalgae.
The effect of Chlorella pyrenoidosa (CP) and Spirulina platensis (SP) at concentrations of 0 %–12 % on the properties of rice starch (RS) was investigated. Compared with pure RS, the addition of CP and SP powder decreased the viscosity, increased the gelatinization temperature, and promoted the retrogradation of RS gel. However, when CP was added at 12 % and SP at 8 %, retrogradation inhibition was reduced. At these concentrations, the relative crystallinity of the CP mixture increased by 57.37 %, whereas that of SP increased by 48.13 %. Scanning electron microscopy revealed that the addition of low amount of CP and SP reduced porosity. CP and SP powder facilitated the conversion of bound water to free water and contributed to the weakening of the viscoelasticity of the RS gel. CP powder likely had a more detrimental effect on the short-term storage properties of RS than SP powder. These results provide theoretical support for the development of RS-based products and the innovative utilization of microalgae.
摘要：
Total starch granule-associated proteins (tGAP), including granule-channel (GCP) and granule-surface proteins (GSP), alter the physicochemical properties of starches. Quinoa starch (QS) acts as an effective emulsifier in Pickering emulsion. However, the correlation between the tGAP and the emulsifying capacity of QS at different scales remains unclear. Herein, GCP and tGAP were selectively removed from QS, namely QS-C and QS-A. Results indicated that the loss of tGAP increased the water permeability and hydrophilicity of the starch particles. Mesoscopically, removing tGAP decreased the diffusion rate and interfacial viscous modulus. Particularly, GSP had a more profound impact on the interfacial modulus than GCP. Microscopically and macroscopically, the loss of tGAP endowed QS with weakened emulsifying ability in terms of emulsions with larger droplet size and diminished rheological properties. Collectively, this work demonstrated that tGAP played an important role in the structural and interfacial properties of QS molecules and the stability of QS-stabilized emulsions.
摘要：
The aim of this work was to develop a fluorescence method based on the polydopamine-polyethyleneimine (PDA-PEI) copolymerization, which was subsequently applied for the determination of 3-monochloropropane-1,2-diol (3-MCPD) in food contact papers (FCMs). PEI could provide an alkaline environment and then react with dopamine (DA) to produce copolymers by Michael addition and Schiff-base reactions. This copolymer has a strong fluorescence emission at 527 nm. We found that amino groups of DA and PEI could also react with 3-MCPD in an alkaline medium, which improved the morphology and fluorescence intensity of PDA-PEI copolymers. The fluorescence intensity of the polymers was linear but inversely proportional to the concentration of 3-MCPD in the range of 10.0–500.0 μg kg−1 and the detection limit was 2 μg kg−1. The standard addition method was used in FCMs to demonstrate the practical applicability and the spiked recoveries ranged from 99.8 to 110.3 %. Finally, the levels of 3-MCPD in different FCMs (n = 70) were determined by the proposed method. The detection frequencies ranged from 25 % to 100 % and both the highest detection frequency and levels were observed in kitchen papers. More than half of the samples did not comply with the limits recommended by the German Federal Institute for Risk Assessment, suggesting that 3-MCPD released from FCMs is a major route of human exposure.
The aim of this work was to develop a fluorescence method based on the polydopamine-polyethyleneimine (PDA-PEI) copolymerization, which was subsequently applied for the determination of 3-monochloropropane-1,2-diol (3-MCPD) in food contact papers (FCMs). PEI could provide an alkaline environment and then react with dopamine (DA) to produce copolymers by Michael addition and Schiff-base reactions. This copolymer has a strong fluorescence emission at 527 nm. We found that amino groups of DA and PEI could also react with 3-MCPD in an alkaline medium, which improved the morphology and fluorescence intensity of PDA-PEI copolymers. The fluorescence intensity of the polymers was linear but inversely proportional to the concentration of 3-MCPD in the range of 10.0–500.0 μg kg−1 and the detection limit was 2 μg kg−1. The standard addition method was used in FCMs to demonstrate the practical applicability and the spiked recoveries ranged from 99.8 to 110.3 %. Finally, the levels of 3-MCPD in different FCMs (n = 70) were determined by the proposed method. The detection frequencies ranged from 25 % to 100 % and both the highest detection frequency and levels were observed in kitchen papers. More than half of the samples did not comply with the limits recommended by the German Federal Institute for Risk Assessment, suggesting that 3-MCPD released from FCMs is a major route of human exposure.
摘要：
Epidemiological evidence on maternal thyroid function disruption by prenatal exposure to perfluorinated and polyfluorinated substances (PFASs) is limited and inconsistent. The study examined the effects of PFASs exposure during early pregnancy on maternal thyroid function [free thyroxine (FT4), thyroid stimulating hormone (TSH), thyroid peroxidase antibodies (TPOAb) and FT4/TSH ratio]. The associations were evaluated using both single and mixed pollutant models, statistical analyses were further utilized in benchmark dose (BMD) estimations to offer critical references for human health risk assessment. Linear regression was used and then Bonferroni correction adjustment was set up to correct for multiple comparisons. The results revealed a significant association between PFHxS exposure and TSH (β = 0.473; 95% CI: 0.180, 0.767). According to BKMR mixed-effects models, PFHxS was significantly positively correlated with TSH at the 25th percentile. PFASs were associated with the FT4/TSH ratio at the 25th to 40th percentile. The BMD value of the increasing FT4 effect induced by PFBA and PFPeA in pregnant women were 6.68 ng/mL and 1.37 ng/mL, respectively. The BMDs were obtained for TSH in the case of PFBA (0.33 ng/mL), PFHxS (0.28 ng/mL). Although BMDL 10 is higher than observed for maternal TSH elevation in animal studies, both studies agree that thyroid homeostasis is the sensitive target. The fact that BMD results at this stage are lower than current exposure levels to PFHxS underscores the urgency of prioritizing endocrine end points in PFASs risk assessment.
Epidemiological evidence on maternal thyroid function disruption by prenatal exposure to perfluorinated and polyfluorinated substances (PFASs) is limited and inconsistent. The study examined the effects of PFASs exposure during early pregnancy on maternal thyroid function [free thyroxine (FT4), thyroid stimulating hormone (TSH), thyroid peroxidase antibodies (TPOAb) and FT4/TSH ratio]. The associations were evaluated using both single and mixed pollutant models, statistical analyses were further utilized in benchmark dose (BMD) estimations to offer critical references for human health risk assessment. Linear regression was used and then Bonferroni correction adjustment was set up to correct for multiple comparisons. The results revealed a significant association between PFHxS exposure and TSH (β = 0.473; 95% CI: 0.180, 0.767). According to BKMR mixed-effects models, PFHxS was significantly positively correlated with TSH at the 25th percentile. PFASs were associated with the FT4/TSH ratio at the 25th to 40th percentile. The BMD value of the increasing FT4 effect induced by PFBA and PFPeA in pregnant women were 6.68 ng/mL and 1.37 ng/mL, respectively. The BMDs were obtained for TSH in the case of PFBA (0.33 ng/mL), PFHxS (0.28 ng/mL). Although BMDL 10 is higher than observed for maternal TSH elevation in animal studies, both studies agree that thyroid homeostasis is the sensitive target. The fact that BMD results at this stage are lower than current exposure levels to PFHxS underscores the urgency of prioritizing endocrine end points in PFASs risk assessment.
作者机构：
[Michael Schmitt] Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany;[Rustam Guliev] Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany;[Jonas Ballmaier; Orlando Guntinas-Lichius] Department of Otorhinolaryngology-Head and Neck Surgery, Jena University Hospital, Jena, Germany;[Chen Liu] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China;[Edoardo Farnesi; Juergen Popp; Dana Cialla-May] Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany<&wdkj&>Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany
通讯机构：
[Dana Cialla-May] I;Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany<&wdkj&>Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany
摘要：
Early detection of head and neck cancer (HNC) is critical for improving prognosis and survival rates. Many cases are diagnosed at advanced stages due to subtle initial symptoms and the complexity of the head and neck anatomy, which complicates clinical examination and biopsy procedures. Therefore, there is an urgent need for non-invasive, reliable, and rapid diagnostic methods suitable for point-of-care (POC) settings. In this study, we applied surface-enhanced Raman spectroscopy (SERS) to develop a rapid screening method for HNC diagnosis using cerumen as the medium. The study aimed to utilize a SERS-based machine learning (ML) approach to distinguish between cerumen samples from healthy individuals and those with HNC. Principal Component Analysis and Linear Discriminant Analysis (PCA-LDA) were performed to analyze and differentiate the cerumen samples. By comparing the SERS spectra of healthy donors with those of HNC patients, we identified SERS spectral features associated with the presence of tumors. The PCA-LDA method successfully classified healthy and HNC cerumen samples with 87.2% accuracy, 87.3% specificity, 87% sensitivity, and a 90% area under the receiver operating characteristic (ROC AUC) curve. This cerumen-SERS-ML workflow proved effective for the rapid identification and evaluation of HNC, offering a promising tool for disease diagnosis.
摘要：
In real-world food safety incidents, hazards are often diverse and coexist simultaneously. Mature single-target detection technologies, while effective, are insufficient for comprehensively evaluating the overall quality and safety of food. As a result, multi-target detection, which enables a more comprehensive assessment of multiple hazards in food, has emerged as a prominent research focus. Nanomaterials have gained significant attention in sensing technologies due to their unique advantages, such as high sensitivity and potential for point-of-care detection. Furthermore, nanomaterials hold great promise for multi-target detection technologies because of their versatile properties, including fluorescence and other characteristics that make them suitable for developing a wide range of detection methods. On the basis of systematically summarizing the research achievements of multi-targets sensing in recent five years, this paper categorizes multi-targets sensing nanoprobes construction strategies into five types according to recognition/interaction relationships between the sensing units and targets. In addition, this review also elaborates some practical cases to further explain the design strategy, detection process and detection system of multi-targets sensing nanoprobes, which provides a novel and directional guidance for food detection and food industry management. Finally, according to the shortcomings of current multi-targets sensing nanoprobes, the prospect and challenges of multi-targets sensing nanoprobes are given.
In real-world food safety incidents, hazards are often diverse and coexist simultaneously. Mature single-target detection technologies, while effective, are insufficient for comprehensively evaluating the overall quality and safety of food. As a result, multi-target detection, which enables a more comprehensive assessment of multiple hazards in food, has emerged as a prominent research focus. Nanomaterials have gained significant attention in sensing technologies due to their unique advantages, such as high sensitivity and potential for point-of-care detection. Furthermore, nanomaterials hold great promise for multi-target detection technologies because of their versatile properties, including fluorescence and other characteristics that make them suitable for developing a wide range of detection methods. On the basis of systematically summarizing the research achievements of multi-targets sensing in recent five years, this paper categorizes multi-targets sensing nanoprobes construction strategies into five types according to recognition/interaction relationships between the sensing units and targets. In addition, this review also elaborates some practical cases to further explain the design strategy, detection process and detection system of multi-targets sensing nanoprobes, which provides a novel and directional guidance for food detection and food industry management. Finally, according to the shortcomings of current multi-targets sensing nanoprobes, the prospect and challenges of multi-targets sensing nanoprobes are given.
期刊：
Environmental Chemistry and Ecotoxicology,2025年7:1587-1597 ISSN：2590-1826
通讯作者：
Liu, X;Zhang, L;Xiang, Q
作者机构：
[Xia, Zhunan; Chen, Shiyi; Liu, Xin] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Liu, Youren] Chengdu Institute of Food Inspection, Irradiation Preservation Key Laboratory of Sichuan Province, Chengdu 611130, China;[Li, Jingguang; Zhang, Lei] NHC Key Lab of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100010, China;[Xiang, Qian] Healthcare-associated Infection Control Center, Sichuan Academy of Medical Sciences, Sichuan People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, China;[Wu, Yongning] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>NHC Key Lab of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100010, China
通讯机构：
[Xin Liu] K;[Qian Xiang] H;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Healthcare-associated Infection Control Center, Sichuan Academy of Medical Sciences, Sichuan People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, China
关键词：
PFASs;Prenatal exposure;Neonatal sex hormone;Bayesian benchmark dose;Toxicogenomics
摘要：
Emerging evidence indicates that prenatal exposure to per - and polyfluoroalkyl substances (PFASs) may adversely affect endocrine function, particularly during critical windows of fetal development. This study evaluated the impact of prenatal PFAS exposure on neonatal sex hormone levels—total testosterone, estradiol, prolactin, and progesterone—in a cohort of newborns from Beijing, China. We quantified 19 individual PFAS compounds and examined their individual and combined effects on neonatal hormone levels using linear regression, restricted cubic spline (RCS) analysis, weighted quantile sum (WQS) regression, and Bayesian benchmark dose (BMD) modeling. Our results revealed significant positive associations between increased exposure to PFOA (β = 0.115, 95 % CI: 0.050–0.180) and total PFAS (β = 0.066, 95 % CI: 0.010–0.122) and elevated testosterone levels. Additionally, PFUdA exposure was positively associated with increased prolactin levels (β = 0.149, 95 % CI: 0.021–0.277). The RCS analysis further demonstrated non-linear dose-response associations for PFOA and total PFAS exposure with testosterone levels. WQS regression indicated significant positive associations between PFAS mixtures and both testosterone ( P = 0.0014) and prolactin ( P = 0.0208), with PFOA, PFUdA, and 6: 2 Cl-PFESA identified as predominant contributors to these hormonal disruptions. Stratified analyses highlighted notable sex-specific modification effects, with significant interactions observed for PFHxS, PFTeDA, and PFOA influencing testosterone levels, and PFHxS affecting progesterone and prolactin (P for interaction <0.05). Bayesian benchmark dose (BMD) analysis yielded exposure threshold estimates, identifying BMD<sub>10</sub> values of 3.72 ng/mL for PFOA and 2.32 ng/mL for PFUdA associated with a 10 % increase in testosterone and prolactin levels, respectively. Furthermore, we integrated toxicogenomics analysis by identifying candidate genes for PFAS and sex hormones using the Comparative Toxicogenomics Database (CTD) and GeneCards databases. The core targets were subsequently confirmed through STRING analysis and visualized with Cytoscape software. To explore potential molecular mechanisms underlying the endocrine-disrupting effects of PFAS, we performed enrichment analyses of these core targets using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Collectively, these findings suggest that prenatal PFAS exposure may disrupt neonatal hormone homeostasis, especially affecting testosterone and prolactin, with distinct sex-specific effects. This study underscores the urgent need for further research into the underlying mechanisms of PFAS-mediated endocrine disruption and highlights the importance of developing public health strategies to minimize prenatal PFAS exposure, thereby protecting fetal development.
Emerging evidence indicates that prenatal exposure to per - and polyfluoroalkyl substances (PFASs) may adversely affect endocrine function, particularly during critical windows of fetal development. This study evaluated the impact of prenatal PFAS exposure on neonatal sex hormone levels—total testosterone, estradiol, prolactin, and progesterone—in a cohort of newborns from Beijing, China. We quantified 19 individual PFAS compounds and examined their individual and combined effects on neonatal hormone levels using linear regression, restricted cubic spline (RCS) analysis, weighted quantile sum (WQS) regression, and Bayesian benchmark dose (BMD) modeling. Our results revealed significant positive associations between increased exposure to PFOA (β = 0.115, 95 % CI: 0.050–0.180) and total PFAS (β = 0.066, 95 % CI: 0.010–0.122) and elevated testosterone levels. Additionally, PFUdA exposure was positively associated with increased prolactin levels (β = 0.149, 95 % CI: 0.021–0.277). The RCS analysis further demonstrated non-linear dose-response associations for PFOA and total PFAS exposure with testosterone levels. WQS regression indicated significant positive associations between PFAS mixtures and both testosterone ( P = 0.0014) and prolactin ( P = 0.0208), with PFOA, PFUdA, and 6: 2 Cl-PFESA identified as predominant contributors to these hormonal disruptions. Stratified analyses highlighted notable sex-specific modification effects, with significant interactions observed for PFHxS, PFTeDA, and PFOA influencing testosterone levels, and PFHxS affecting progesterone and prolactin (P for interaction <0.05). Bayesian benchmark dose (BMD) analysis yielded exposure threshold estimates, identifying BMD<sub>10</sub> values of 3.72 ng/mL for PFOA and 2.32 ng/mL for PFUdA associated with a 10 % increase in testosterone and prolactin levels, respectively. Furthermore, we integrated toxicogenomics analysis by identifying candidate genes for PFAS and sex hormones using the Comparative Toxicogenomics Database (CTD) and GeneCards databases. The core targets were subsequently confirmed through STRING analysis and visualized with Cytoscape software. To explore potential molecular mechanisms underlying the endocrine-disrupting effects of PFAS, we performed enrichment analyses of these core targets using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Collectively, these findings suggest that prenatal PFAS exposure may disrupt neonatal hormone homeostasis, especially affecting testosterone and prolactin, with distinct sex-specific effects. This study underscores the urgent need for further research into the underlying mechanisms of PFAS-mediated endocrine disruption and highlights the importance of developing public health strategies to minimize prenatal PFAS exposure, thereby protecting fetal development.
关键词：
Gel properties;Gelatinizing properties;Microstructure;Wheat starch;gWPI
摘要：
Glycated whey protein isolate (gWPI) was produced by dry thermal reaction between whey protein isolate (WPI) and lactose, and its influence on the gelatinization, gel properties, and microstructure changes of wheat starch (WS) was systematically studied. Results showed that gWPI significantly inhibited starch gelatinization and improved starch gel properties. RVA and DSC analysis showed that gWPI decreased viscosity and gelatinization enthalpy (ΔH) in a concentration-dependent manner. When gWPI concentration was 12 %, the peak viscosity and ΔH decreased by 36 cP and 3.11 J/g, respectively. gWPI competed with WS for water and inhibited WS water absorption and expansion. Rheological results showed that the viscoelasticity of the gel decreased by adding gWPI and that of WS-gWPI was a pseudoplastic fluid with shear-thinning behavior. In addition, gWPI covered the WS surface in the form of hydrogen bonds, which inhibited the leaching of amylose, thereby reducing the particle size of the gelatinized starch and its iodine binding ability. AFM results showed that gWPI combined with WS weakened the aggregation of starch molecular chains and decreased the height of starch. In conclusion, gWPI can be used as a gelatinizing regulator to improve starch properties.
Glycated whey protein isolate (gWPI) was produced by dry thermal reaction between whey protein isolate (WPI) and lactose, and its influence on the gelatinization, gel properties, and microstructure changes of wheat starch (WS) was systematically studied. Results showed that gWPI significantly inhibited starch gelatinization and improved starch gel properties. RVA and DSC analysis showed that gWPI decreased viscosity and gelatinization enthalpy (ΔH) in a concentration-dependent manner. When gWPI concentration was 12 %, the peak viscosity and ΔH decreased by 36 cP and 3.11 J/g, respectively. gWPI competed with WS for water and inhibited WS water absorption and expansion. Rheological results showed that the viscoelasticity of the gel decreased by adding gWPI and that of WS-gWPI was a pseudoplastic fluid with shear-thinning behavior. In addition, gWPI covered the WS surface in the form of hydrogen bonds, which inhibited the leaching of amylose, thereby reducing the particle size of the gelatinized starch and its iodine binding ability. AFM results showed that gWPI combined with WS weakened the aggregation of starch molecular chains and decreased the height of starch. In conclusion, gWPI can be used as a gelatinizing regulator to improve starch properties.
摘要：
The effects of different phenolic compounds (Epigallocatechin gallate, EGCG; Catechin, CC; Tannic acid, TA) at different phenol hydroxyl concentrations on the gel strength, water holding capacity (WHC), water distribution, and micro-morphology of surimi gels were investigated. Breaking force and deformation of surimi gels treated with EGCG, CC, and TA were increased by 20%, 15%, 12% and 14%, 7%, 4%, compared with untreated surimi gel. Polyphenols increased the WHC of surimi gels and induced the conversion of α-helix to β-sheet in proteins, which was beneficial to the gelation of surimi gel. Surimi gels treated with polyphenols had a complete three-dimensional network structure and relatively uniform and small pores, especially EGCG cross-linked surimi gel. Molecular docking indicated that the interactions between EGCG, CC, TA and myosin heavy chain II A were mainly hydrogen bond and hydrophobic interaction, and their binding energies were −37, −31, and −26 kJ/mol. The binding energy of polyphenols with myosin heavy chain II A correlates with their cross-linking ability with proteins, and the lower the binding energy is, the stronger the cross-linking ability is. This study provides important information to elucidate the mechanism of phenolic compounds regulating the physical properties of surimi gel.
The effects of different phenolic compounds (Epigallocatechin gallate, EGCG; Catechin, CC; Tannic acid, TA) at different phenol hydroxyl concentrations on the gel strength, water holding capacity (WHC), water distribution, and micro-morphology of surimi gels were investigated. Breaking force and deformation of surimi gels treated with EGCG, CC, and TA were increased by 20%, 15%, 12% and 14%, 7%, 4%, compared with untreated surimi gel. Polyphenols increased the WHC of surimi gels and induced the conversion of α-helix to β-sheet in proteins, which was beneficial to the gelation of surimi gel. Surimi gels treated with polyphenols had a complete three-dimensional network structure and relatively uniform and small pores, especially EGCG cross-linked surimi gel. Molecular docking indicated that the interactions between EGCG, CC, TA and myosin heavy chain II A were mainly hydrogen bond and hydrophobic interaction, and their binding energies were −37, −31, and −26 kJ/mol. The binding energy of polyphenols with myosin heavy chain II A correlates with their cross-linking ability with proteins, and the lower the binding energy is, the stronger the cross-linking ability is. This study provides important information to elucidate the mechanism of phenolic compounds regulating the physical properties of surimi gel.
摘要：
Fractionation allows the separation of components in beef tallow. This study compared the physicochemical characteristics and cholesterol content of beef tallow and its liquid fraction, evaluating their frying performance as potential deep-fat frying oils against plant oils. Results showed effective separation of unsaturated components from beef tallow through fractionation. Beef tallow exhibited superior physicochemical properties during frying, with lower deterioration levels than plant oils. Benzo[ a ]pyrene content increased in plant oils but remained low in beef tallow and its liquid fraction. The liquid fraction had a significantly shorter oxidative induction time of 0.38 h compared to 5.85 h and 5.24 h for plant oils. This study revealed that alterations were observed in beef tallow and its liquid fraction when used as frying oils, with beef tallow demonstrating stronger antioxidative properties compared to the liquid fraction, which exhibited lower levels of cholesterol and saturated fatty acids.
Fractionation allows the separation of components in beef tallow. This study compared the physicochemical characteristics and cholesterol content of beef tallow and its liquid fraction, evaluating their frying performance as potential deep-fat frying oils against plant oils. Results showed effective separation of unsaturated components from beef tallow through fractionation. Beef tallow exhibited superior physicochemical properties during frying, with lower deterioration levels than plant oils. Benzo[ a ]pyrene content increased in plant oils but remained low in beef tallow and its liquid fraction. The liquid fraction had a significantly shorter oxidative induction time of 0.38 h compared to 5.85 h and 5.24 h for plant oils. This study revealed that alterations were observed in beef tallow and its liquid fraction when used as frying oils, with beef tallow demonstrating stronger antioxidative properties compared to the liquid fraction, which exhibited lower levels of cholesterol and saturated fatty acids.
摘要：
To elucidate the volatile flavor compositions and overall aroma profiles of fermented bean curd (FBC), the volatile organic compounds (VOCs) in six different FBC were detected and characterized using gas chromatography-ion mobility spectrometry (GC-IMS) and an electronic nose (E-nose) in this study. A total of 60 VOCs were identified by GC-IMS, of which esters, aldehydes, alcohols, and ketones constituted the major compounds. Among them, 17 VOCs were identified as key differentiating volatile compounds. In addition, the E-nose combined two algorithms, Linear Discriminant Analysis (LDA) and K-Nearest Neighbor (KNN), to demonstrate its effectiveness in differentiating between different FBC samples. The results showed that the LDA model performed better than the KNN model. When the principal component number was 9, the recognition accuracies of the training and prediction sets for the LDA model were 94.44% and 91.67%, respectively. In addition, a multi-channel colorimetric sensor array (CSA) was constructed in this study for the quantitative prediction of key physicochemical indicators. The results showed that both partial least squares regression (PLSR) and support vector machine regression (SVR) achieved good prediction performance. Among them, for the SVR model, the prediction correlation coefficients for total acidity, reducing sugar, salinity, and amino acid nitrogen were 0.9033, 0.9170, 0.7298, and 0.9213, respectively. The results of this study indicate that GC-IMS, E-nose, and CSA are expected to be effective tools for characterizing FBC flavor as well as facilitating the rapid quantification of key physicochemical indicators, which may provide valuable insights for flavor and quality control in traditional fermented foods.
To elucidate the volatile flavor compositions and overall aroma profiles of fermented bean curd (FBC), the volatile organic compounds (VOCs) in six different FBC were detected and characterized using gas chromatography-ion mobility spectrometry (GC-IMS) and an electronic nose (E-nose) in this study. A total of 60 VOCs were identified by GC-IMS, of which esters, aldehydes, alcohols, and ketones constituted the major compounds. Among them, 17 VOCs were identified as key differentiating volatile compounds. In addition, the E-nose combined two algorithms, Linear Discriminant Analysis (LDA) and K-Nearest Neighbor (KNN), to demonstrate its effectiveness in differentiating between different FBC samples. The results showed that the LDA model performed better than the KNN model. When the principal component number was 9, the recognition accuracies of the training and prediction sets for the LDA model were 94.44% and 91.67%, respectively. In addition, a multi-channel colorimetric sensor array (CSA) was constructed in this study for the quantitative prediction of key physicochemical indicators. The results showed that both partial least squares regression (PLSR) and support vector machine regression (SVR) achieved good prediction performance. Among them, for the SVR model, the prediction correlation coefficients for total acidity, reducing sugar, salinity, and amino acid nitrogen were 0.9033, 0.9170, 0.7298, and 0.9213, respectively. The results of this study indicate that GC-IMS, E-nose, and CSA are expected to be effective tools for characterizing FBC flavor as well as facilitating the rapid quantification of key physicochemical indicators, which may provide valuable insights for flavor and quality control in traditional fermented foods.
作者机构：
[Wang, Ziying; Liu, Haiqing; Weng, Yunxiang; Fang, Yan; Zheng, Xinwei; Chen, Qinhui] Fujian Normal Univ, Coll Chem & Mat Sci, Fujian Prov Key Lab Polymer Mat, Fuzhou 350007, Fujian, Peoples R China.;[Sun, Xun] Xuzhou Vocat Coll Ind Technol, Coll Mat Engn, Xuzhou 221000, Peoples R China.;[Fan, Xianmou] Guangdong Med Univ, Affiliated Hosp, Dept Plast Surg, Zhanjiang 524000, Guangdong, Peoples R China.;[Wang, Guozhen] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Sun, X ] X;[Chen, QH; Fang, Y ; Liu, HQ] F;[Fan, XM ] G;Fujian Normal Univ, Coll Chem & Mat Sci, Fujian Prov Key Lab Polymer Mat, Fuzhou 350007, Fujian, Peoples R China.;Xuzhou Vocat Coll Ind Technol, Coll Mat Engn, Xuzhou 221000, Peoples R China.
关键词：
Cotton gauze;Hemostasis;Polysaccharide
摘要：
Cotton gauze remains a clinically prevalent hemostatic material, yet its effectiveness remains compromised by bleeding. We have deposited cationic quaternized chitosan (QCS) and anionic catechol-functionalized hyaluronic acid (HADA) nanofibers onto cotton gauze to prepare QCS/HADA@gauze. Upon blood contact, oppositely charged nanofibers electrostatically adhere through complementary charge interactions. The interfiber complexation achieves dual hemostatic mechanisms by rapidly establishing a physical barrier while concurrently entrapping circulating blood components (erythrocytes and platelets). Furthermore, the QCS/HADA@gauze can adhere firmly to the tissue, preventing bleeding through the gap between the gauze and the tissue. Additionally, the QCS/HADA@gauze can be removed easily when needed by using NaCl solution. QCS/HADA@gauze exhibits superior coagulation and hemostatic performance than both cotton gauze and Combat Gauze™. Furthermore, QCS/HADA@gauze has exceptional biocompatibility and antibacterial activity. Our study confirms that this strategy for designing hemostatic fabric has considerable potential.
Cotton gauze remains a clinically prevalent hemostatic material, yet its effectiveness remains compromised by bleeding. We have deposited cationic quaternized chitosan (QCS) and anionic catechol-functionalized hyaluronic acid (HADA) nanofibers onto cotton gauze to prepare QCS/HADA@gauze. Upon blood contact, oppositely charged nanofibers electrostatically adhere through complementary charge interactions. The interfiber complexation achieves dual hemostatic mechanisms by rapidly establishing a physical barrier while concurrently entrapping circulating blood components (erythrocytes and platelets). Furthermore, the QCS/HADA@gauze can adhere firmly to the tissue, preventing bleeding through the gap between the gauze and the tissue. Additionally, the QCS/HADA@gauze can be removed easily when needed by using NaCl solution. QCS/HADA@gauze exhibits superior coagulation and hemostatic performance than both cotton gauze and Combat Gauze™. Furthermore, QCS/HADA@gauze has exceptional biocompatibility and antibacterial activity. Our study confirms that this strategy for designing hemostatic fabric has considerable potential.
摘要：
It is an urgent need of rapid and sensitive method for detection of Escherichia coli O157:H7 ( E. coli O157:H7), a class of hazardous foodborne pathogens in food safety. The traditional enzyme-linked immunosorbent assay (ELISA), a dominant rapid detection technic, takes disadvantages of low test sensitivity due to the insufficient enzyme loading capacity. In this study, we successfully synthesized the self-assembled Au/polydopamine (PDA)/HRP nanocomposites with the high enzyme loading on the outer surface and in the inner space. The high catalytic activity of Au/PDA/HRP was maintained by virtue of its hyperbranched flexible structure. For E. coli O157:H7 detection in milk samples, the proposed immunoassay achieved a visual cut-off value of 10 3 cfu mL −1 and a low limit of detection (LOD) of 2.8 × 10 2 cfu mL −1 , 33 and 46 times more sensitive than the traditional ELISA, respectively. The tremendous advantages of high sensitivity, excellent specificity and adequate recovery make it promising for sensitively monitoring various kinds of pathogenic bacteria in food safety.
It is an urgent need of rapid and sensitive method for detection of Escherichia coli O157:H7 ( E. coli O157:H7), a class of hazardous foodborne pathogens in food safety. The traditional enzyme-linked immunosorbent assay (ELISA), a dominant rapid detection technic, takes disadvantages of low test sensitivity due to the insufficient enzyme loading capacity. In this study, we successfully synthesized the self-assembled Au/polydopamine (PDA)/HRP nanocomposites with the high enzyme loading on the outer surface and in the inner space. The high catalytic activity of Au/PDA/HRP was maintained by virtue of its hyperbranched flexible structure. For E. coli O157:H7 detection in milk samples, the proposed immunoassay achieved a visual cut-off value of 10 3 cfu mL −1 and a low limit of detection (LOD) of 2.8 × 10 2 cfu mL −1 , 33 and 46 times more sensitive than the traditional ELISA, respectively. The tremendous advantages of high sensitivity, excellent specificity and adequate recovery make it promising for sensitively monitoring various kinds of pathogenic bacteria in food safety.
摘要：
Some probiotic films have not shown satisfactory antibacterial performance, which limits their application in food. The purpose of this study is to improve the antibacterial activity of probiotic film by using probiotics to ferment the film solution, and to solve the problem of weak antibacterial performance of probiotic film. In this study, the solution composed of gum arabic/whey protein isolate/isomalt/glycerol was firstly fermented by Lactobacillus rhamnosus (L. rhamnosus) HN001, and then prepared into probiotic fermented films. The effect of probiotic fermentation on the films properties was systematically evaluated in this study. These results demonstrated that probiotic fermentation enhanced the mechanical strength, water barrier properties, thermal stability, and antioxidant activity of the films. According to the scanning electron microscopy (SEM), the probiotic-fermented films exhibited a more compact and coherent structure. Characterization of the films revealed that probiotic fermentation improved the compatibility between the film components and altered the conformation of whey protein. Compared to the non-fermented probiotic films, the probiotic-fermented films exhibited an 11% increase in the inhibition rate against Staphylococcus aureus and a 21% increase in the inhibition rate against Salmonella. The probiotic-fermented films successfully delayed the spoilage of fresh pork stored at 4 °C, effectively inhibiting microbial growth and reproduction, demonstrating a more effective preservation effect than the non-fermented probiotic films. This study provides an effective and convenient method for improving the antibacterial properties and preservation effect of probiotic films.
Some probiotic films have not shown satisfactory antibacterial performance, which limits their application in food. The purpose of this study is to improve the antibacterial activity of probiotic film by using probiotics to ferment the film solution, and to solve the problem of weak antibacterial performance of probiotic film. In this study, the solution composed of gum arabic/whey protein isolate/isomalt/glycerol was firstly fermented by Lactobacillus rhamnosus (L. rhamnosus) HN001, and then prepared into probiotic fermented films. The effect of probiotic fermentation on the films properties was systematically evaluated in this study. These results demonstrated that probiotic fermentation enhanced the mechanical strength, water barrier properties, thermal stability, and antioxidant activity of the films. According to the scanning electron microscopy (SEM), the probiotic-fermented films exhibited a more compact and coherent structure. Characterization of the films revealed that probiotic fermentation improved the compatibility between the film components and altered the conformation of whey protein. Compared to the non-fermented probiotic films, the probiotic-fermented films exhibited an 11% increase in the inhibition rate against Staphylococcus aureus and a 21% increase in the inhibition rate against Salmonella. The probiotic-fermented films successfully delayed the spoilage of fresh pork stored at 4 °C, effectively inhibiting microbial growth and reproduction, demonstrating a more effective preservation effect than the non-fermented probiotic films. This study provides an effective and convenient method for improving the antibacterial properties and preservation effect of probiotic films.
摘要：
Flupyradifurone is a novel butenolide insecticide widely applied to control a broad range of sucking pests, including Myzus persicae . However, resistance monitoring has revealed that several Chinese populations have developed moderate resistance to flupyradifurone. Therefore, we investigated the risk and underlying mechanisms of flupyradifurone resistance in M. persicae . A flupyradifurone-resistant strain (FDF-R) was established through 14 generations of selection and exhibited a 35.02-fold increase in resistance. FDF-R showed cross-resistance to acetamiprid (13.9-fold), sulfoxaflor (3.5-fold), and imidacloprid (4.2-fold), but not to tested pyrethroid or carbamate insecticides. The synergist piperonyl butoxide significantly increased flupyradifurone toxicity in FDF-R, suggesting the involvement of cytochrome P450 monooxygenases, whereas the other two synergists had no effect. Enzyme assays revealed 1.74-fold higher P450 activity in FDF-R compared to the susceptible strain. Gene expression analysis showed significant overexpression of seven P450 genes, including CYP380C34 , CYP6CY36 , CYP6CY4 , and CYP6CY3 . Moreover, RNAi silencing of CYP6CY36 and CYP380C34 significantly increased susceptibility to flupyradifurone. Additionally, molecular docking further confirmed strong binding affinities between these two P450 enzymes and flupyradifurone. These findings demonstrate that P450-mediated detoxification contributes to M. persicae resistance to flupyradifurone.
Flupyradifurone is a novel butenolide insecticide widely applied to control a broad range of sucking pests, including Myzus persicae . However, resistance monitoring has revealed that several Chinese populations have developed moderate resistance to flupyradifurone. Therefore, we investigated the risk and underlying mechanisms of flupyradifurone resistance in M. persicae . A flupyradifurone-resistant strain (FDF-R) was established through 14 generations of selection and exhibited a 35.02-fold increase in resistance. FDF-R showed cross-resistance to acetamiprid (13.9-fold), sulfoxaflor (3.5-fold), and imidacloprid (4.2-fold), but not to tested pyrethroid or carbamate insecticides. The synergist piperonyl butoxide significantly increased flupyradifurone toxicity in FDF-R, suggesting the involvement of cytochrome P450 monooxygenases, whereas the other two synergists had no effect. Enzyme assays revealed 1.74-fold higher P450 activity in FDF-R compared to the susceptible strain. Gene expression analysis showed significant overexpression of seven P450 genes, including CYP380C34 , CYP6CY36 , CYP6CY4 , and CYP6CY3 . Moreover, RNAi silencing of CYP6CY36 and CYP380C34 significantly increased susceptibility to flupyradifurone. Additionally, molecular docking further confirmed strong binding affinities between these two P450 enzymes and flupyradifurone. These findings demonstrate that P450-mediated detoxification contributes to M. persicae resistance to flupyradifurone.
作者机构：
[Xun Pei; Zushan Tan; Yilin Li; Muci Wu; Wangting Zhou] School of Modern Industry for Selenium Science and Engineering, National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China;[Jinzeng Wang] Key Laboratory of Magnetic Molecules & Magnetic Information Materials Ministry of Education, School of Chemistry & Material Science, Shanxi Normal University, Taiyuan 030031, China;[Chen Liu] School of Food Science and Engineering, Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China;[Nabil Grimi] Université de Technologie de Compiègne UTC/ESCOM, Laboratoire Transformations Intégrées de la Matière Renouvelable (TIMR), Compiègne 60203, France;[Jingren He; Rui Zhang] School of Modern Industry for Selenium Science and Engineering, National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Food Science and Engineering, Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Chen Liu; Rui Zhang] S;School of Modern Industry for Selenium Science and Engineering, National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Food Science and Engineering, Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Food Science and Engineering, Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
This study investigated the effect of pulsed electric field (PEF, E = 0–6 kV/cm, t = 0–5.65 ms) on cell disruption and selective extraction of intracellular components from selenium-enriched Konjac fly powder, in comparison with high pressure homogenization (HPH). The intracellular components extractability and cell disruption degree were evaluated by extraction indexes and cell disruption index, respectively. Results showed that PEF significantly improved cell disruption and components release. The extraction indexes increased with higher E and t . The extractability order was ionic components > carbohydrates > proteins, while the maximum selenium level was obtained at 1.13 ms. Compared to HPH, PEF favored carbohydrates extraction over proteins. For example, at 3 kJ/g, PEF gave a selectivity index ( S ) ≈ 3.0 (4 kV/cm) and ≈ 4.2 (6 kV/cm), while HPH gave S ≈ 2.3. Non-linear relationships between extraction indexes and cell disruption index reflected PEF differential effects on cell membranes and walls.
This study investigated the effect of pulsed electric field (PEF, E = 0–6 kV/cm, t = 0–5.65 ms) on cell disruption and selective extraction of intracellular components from selenium-enriched Konjac fly powder, in comparison with high pressure homogenization (HPH). The intracellular components extractability and cell disruption degree were evaluated by extraction indexes and cell disruption index, respectively. Results showed that PEF significantly improved cell disruption and components release. The extraction indexes increased with higher E and t . The extractability order was ionic components > carbohydrates > proteins, while the maximum selenium level was obtained at 1.13 ms. Compared to HPH, PEF favored carbohydrates extraction over proteins. For example, at 3 kJ/g, PEF gave a selectivity index ( S ) ≈ 3.0 (4 kV/cm) and ≈ 4.2 (6 kV/cm), while HPH gave S ≈ 2.3. Non-linear relationships between extraction indexes and cell disruption index reflected PEF differential effects on cell membranes and walls.
