作者机构：
[Michael Schmitt] Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany;[Rustam Guliev] Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany;[Jonas Ballmaier; Orlando Guntinas-Lichius] Department of Otorhinolaryngology-Head and Neck Surgery, Jena University Hospital, Jena, Germany;[Chen Liu] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China;[Edoardo Farnesi; Juergen Popp; Dana Cialla-May] Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany<&wdkj&>Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany
通讯机构：
[Dana Cialla-May] I;Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany<&wdkj&>Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany
摘要：
Early detection of head and neck cancer (HNC) is critical for improving prognosis and survival rates. Many cases are diagnosed at advanced stages due to subtle initial symptoms and the complexity of the head and neck anatomy, which complicates clinical examination and biopsy procedures. Therefore, there is an urgent need for non-invasive, reliable, and rapid diagnostic methods suitable for point-of-care (POC) settings. In this study, we applied surface-enhanced Raman spectroscopy (SERS) to develop a rapid screening method for HNC diagnosis using cerumen as the medium. The study aimed to utilize a SERS-based machine learning (ML) approach to distinguish between cerumen samples from healthy individuals and those with HNC. Principal Component Analysis and Linear Discriminant Analysis (PCA-LDA) were performed to analyze and differentiate the cerumen samples. By comparing the SERS spectra of healthy donors with those of HNC patients, we identified SERS spectral features associated with the presence of tumors. The PCA-LDA method successfully classified healthy and HNC cerumen samples with 87.2% accuracy, 87.3% specificity, 87% sensitivity, and a 90% area under the receiver operating characteristic (ROC AUC) curve. This cerumen-SERS-ML workflow proved effective for the rapid identification and evaluation of HNC, offering a promising tool for disease diagnosis.
摘要：
In real-world food safety incidents, hazards are often diverse and coexist simultaneously. Mature single-target detection technologies, while effective, are insufficient for comprehensively evaluating the overall quality and safety of food. As a result, multi-target detection, which enables a more comprehensive assessment of multiple hazards in food, has emerged as a prominent research focus. Nanomaterials have gained significant attention in sensing technologies due to their unique advantages, such as high sensitivity and potential for point-of-care detection. Furthermore, nanomaterials hold great promise for multi-target detection technologies because of their versatile properties, including fluorescence and other characteristics that make them suitable for developing a wide range of detection methods. On the basis of systematically summarizing the research achievements of multi-targets sensing in recent five years, this paper categorizes multi-targets sensing nanoprobes construction strategies into five types according to recognition/interaction relationships between the sensing units and targets. In addition, this review also elaborates some practical cases to further explain the design strategy, detection process and detection system of multi-targets sensing nanoprobes, which provides a novel and directional guidance for food detection and food industry management. Finally, according to the shortcomings of current multi-targets sensing nanoprobes, the prospect and challenges of multi-targets sensing nanoprobes are given.
In real-world food safety incidents, hazards are often diverse and coexist simultaneously. Mature single-target detection technologies, while effective, are insufficient for comprehensively evaluating the overall quality and safety of food. As a result, multi-target detection, which enables a more comprehensive assessment of multiple hazards in food, has emerged as a prominent research focus. Nanomaterials have gained significant attention in sensing technologies due to their unique advantages, such as high sensitivity and potential for point-of-care detection. Furthermore, nanomaterials hold great promise for multi-target detection technologies because of their versatile properties, including fluorescence and other characteristics that make them suitable for developing a wide range of detection methods. On the basis of systematically summarizing the research achievements of multi-targets sensing in recent five years, this paper categorizes multi-targets sensing nanoprobes construction strategies into five types according to recognition/interaction relationships between the sensing units and targets. In addition, this review also elaborates some practical cases to further explain the design strategy, detection process and detection system of multi-targets sensing nanoprobes, which provides a novel and directional guidance for food detection and food industry management. Finally, according to the shortcomings of current multi-targets sensing nanoprobes, the prospect and challenges of multi-targets sensing nanoprobes are given.
摘要：
The potential health effects of exposure to rare earth elements (REEs) remain largely unexplored. This prospective cohort study aimed to elucidate the association between early pregnancy REE exposure and maternal thyroid function, as well as neonatal birth outcomes, in a cohort of pregnant women in Beijing, China. Additionally, the study explored the mediating role of thyroid homeostasis in the effects of REE exposure. Serum concentrations of fifteen REEs, along with Free Thyroxine (FT4), Thyroid Stimulating Hormone (TSH), and Thyroid Peroxidase Antibodies (TPOAb), were measured in 195 pregnant women. Multivariable linear regression analyses identified significant correlations between REE exposure and disruptions in maternal thyroid homeostasis. Specifically, Praseodymium (Pr) and Lutetium (Lu) were positively associated with FT4 levels, while Gadolinium (Gd) showed a positive correlation with TSH levels. Conversely, Thulium (Tm) was negatively associated with FT4 levels, and Yttrium (Y) was negatively correlated with TSH levels, indicating distinct interactions of specific REEs with thyroid regulation. Notably, Lu remained positively correlated with FT4 levels (β = 1.39, 95% CI = 0.55, 2.22) after adjusting for multiple comparisons. Regarding neonatal birth outcomes, Dysprosium (Dy) was found to be negatively associated with infant birth weight (β = −0.09, 95% CI = −0.170, −0.002). Furthermore, gender-specific analyses revealed significant associations between REE exposure and TPOAb levels among female neonates. Mediation analyses indicated that TSH significantly mediated the relationships between Terbium (Tb) and Y exposure and neonatal birth outcomes. The study suggests that REEs may disrupt endocrine function, particularly thyroid hormones, which could adversely affect neonatal growth, highlighting the need for further research on their impact in vulnerable populations.
The potential health effects of exposure to rare earth elements (REEs) remain largely unexplored. This prospective cohort study aimed to elucidate the association between early pregnancy REE exposure and maternal thyroid function, as well as neonatal birth outcomes, in a cohort of pregnant women in Beijing, China. Additionally, the study explored the mediating role of thyroid homeostasis in the effects of REE exposure. Serum concentrations of fifteen REEs, along with Free Thyroxine (FT4), Thyroid Stimulating Hormone (TSH), and Thyroid Peroxidase Antibodies (TPOAb), were measured in 195 pregnant women. Multivariable linear regression analyses identified significant correlations between REE exposure and disruptions in maternal thyroid homeostasis. Specifically, Praseodymium (Pr) and Lutetium (Lu) were positively associated with FT4 levels, while Gadolinium (Gd) showed a positive correlation with TSH levels. Conversely, Thulium (Tm) was negatively associated with FT4 levels, and Yttrium (Y) was negatively correlated with TSH levels, indicating distinct interactions of specific REEs with thyroid regulation. Notably, Lu remained positively correlated with FT4 levels (β = 1.39, 95% CI = 0.55, 2.22) after adjusting for multiple comparisons.
Regarding neonatal birth outcomes, Dysprosium (Dy) was found to be negatively associated with infant birth weight (β = −0.09, 95% CI = −0.170, −0.002). Furthermore, gender-specific analyses revealed significant associations between REE exposure and TPOAb levels among female neonates. Mediation analyses indicated that TSH significantly mediated the relationships between Terbium (Tb) and Y exposure and neonatal birth outcomes. The study suggests that REEs may disrupt endocrine function, particularly thyroid hormones, which could adversely affect neonatal growth, highlighting the need for further research on their impact in vulnerable populations.
摘要：
Some probiotic films have not shown satisfactory antibacterial performance, which limits their application in food. The purpose of this study is to improve the antibacterial activity of probiotic film by using probiotics to ferment the film solution, and to solve the problem of weak antibacterial performance of probiotic film. In this study, the solution composed of gum arabic/whey protein isolate/isomalt/glycerol was firstly fermented by Lactobacillus rhamnosus (L. rhamnosus) HN001, and then prepared into probiotic fermented films. The effect of probiotic fermentation on the films properties was systematically evaluated in this study. These results demonstrated that probiotic fermentation enhanced the mechanical strength, water barrier properties, thermal stability, and antioxidant activity of the films. According to the scanning electron microscopy (SEM), the probiotic-fermented films exhibited a more compact and coherent structure. Characterization of the films revealed that probiotic fermentation improved the compatibility between the film components and altered the conformation of whey protein. Compared to the non-fermented probiotic films, the probiotic-fermented films exhibited an 11% increase in the inhibition rate against Staphylococcus aureus and a 21% increase in the inhibition rate against Salmonella. The probiotic-fermented films successfully delayed the spoilage of fresh pork stored at 4 °C, effectively inhibiting microbial growth and reproduction, demonstrating a more effective preservation effect than the non-fermented probiotic films. This study provides an effective and convenient method for improving the antibacterial properties and preservation effect of probiotic films.
Some probiotic films have not shown satisfactory antibacterial performance, which limits their application in food. The purpose of this study is to improve the antibacterial activity of probiotic film by using probiotics to ferment the film solution, and to solve the problem of weak antibacterial performance of probiotic film. In this study, the solution composed of gum arabic/whey protein isolate/isomalt/glycerol was firstly fermented by Lactobacillus rhamnosus (L. rhamnosus) HN001, and then prepared into probiotic fermented films. The effect of probiotic fermentation on the films properties was systematically evaluated in this study. These results demonstrated that probiotic fermentation enhanced the mechanical strength, water barrier properties, thermal stability, and antioxidant activity of the films. According to the scanning electron microscopy (SEM), the probiotic-fermented films exhibited a more compact and coherent structure. Characterization of the films revealed that probiotic fermentation improved the compatibility between the film components and altered the conformation of whey protein. Compared to the non-fermented probiotic films, the probiotic-fermented films exhibited an 11% increase in the inhibition rate against Staphylococcus aureus and a 21% increase in the inhibition rate against Salmonella. The probiotic-fermented films successfully delayed the spoilage of fresh pork stored at 4 °C, effectively inhibiting microbial growth and reproduction, demonstrating a more effective preservation effect than the non-fermented probiotic films. This study provides an effective and convenient method for improving the antibacterial properties and preservation effect of probiotic films.
摘要：
Total starch granule-associated proteins (tGAP), including granule-channel (GCP) and granule-surface proteins (GSP), alter the physicochemical properties of starches. Quinoa starch (QS) acts as an effective emulsifier in Pickering emulsion. However, the correlation between the tGAP and the emulsifying capacity of QS at different scales remains unclear. Herein, GCP and tGAP were selectively removed from QS, namely QS-C and QS-A. Results indicated that the loss of tGAP increased the water permeability and hydrophilicity of the starch particles. Mesoscopically, removing tGAP decreased the diffusion rate and interfacial viscous modulus. Particularly, GSP had a more profound impact on the interfacial modulus than GCP. Microscopically and macroscopically, the loss of tGAP endowed QS with weakened emulsifying ability in terms of emulsions with larger droplet size and diminished rheological properties. Collectively, this work demonstrated that tGAP played an important role in the structural and interfacial properties of QS molecules and the stability of QS-stabilized emulsions.
作者机构：
[Fang, Min] Key Laboratory of Edible Oil Quality and Safety, State Administration for Market Regulation, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, 68Xuefu South Road, Changqing Garden, Wuhan, 430023, China. Electronic address: fangmin0227@126.com;[Gong, Zhiyong; Wang, Zhaojie; Xu, Lin; Fu, Qing; Yang, Qing] Key Laboratory of Edible Oil Quality and Safety, State Administration for Market Regulation, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, 68Xuefu South Road, Changqing Garden, Wuhan, 430023, China;[Jiang, Xiaoming; Lu, Yuepeng; Yang, Yong] Wuhan Institute for Food and Cosmetic Control, 1137 Jinshan Avenue, Wuhan, 430012, China;[Wu, Yongning] Research Unit of Food Safety, Chinese Academy of Medical Sciences (No. 2019RU014), NHC Key Lab of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment (CFSA), Beijing 100022, China;[Liu, Xin] Key Laboratory of Edible Oil Quality and Safety, State Administration for Market Regulation, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, 68Xuefu South Road, Changqing Garden, Wuhan, 430023, China. Electronic address: liuxinhook@whpu.edu.cn
通讯机构：
[Min Fang] K;Key Laboratory of Edible Oil Quality and Safety, State Administration for Market Regulation, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, 68Xuefu South Road, Changqing Garden, Wuhan, 430023, China
摘要：
The aim of this work was to develop a fluorescence method based on the polydopamine-polyethyleneimine (PDA-PEI) copolymerization, which was subsequently applied for the determination of 3-monochloropropane-1,2-diol (3-MCPD) in food contact papers (FCMs). PEI could provide an alkaline environment and then react with dopamine (DA) to produce copolymers by Michael addition and Schiff-base reactions. This copolymer has a strong fluorescence emission at 527 nm. We found that amino groups of DA and PEI could also react with 3-MCPD in an alkaline medium, which improved the morphology and fluorescence intensity of PDA-PEI copolymers. The fluorescence intensity of the polymers was linear but inversely proportional to the concentration of 3-MCPD in the range of 10.0–500.0 μg kg−1 and the detection limit was 2 μg kg−1. The standard addition method was used in FCMs to demonstrate the practical applicability and the spiked recoveries ranged from 99.8 to 110.3 %. Finally, the levels of 3-MCPD in different FCMs (n = 70) were determined by the proposed method. The detection frequencies ranged from 25 % to 100 % and both the highest detection frequency and levels were observed in kitchen papers. More than half of the samples did not comply with the limits recommended by the German Federal Institute for Risk Assessment, suggesting that 3-MCPD released from FCMs is a major route of human exposure.
The aim of this work was to develop a fluorescence method based on the polydopamine-polyethyleneimine (PDA-PEI) copolymerization, which was subsequently applied for the determination of 3-monochloropropane-1,2-diol (3-MCPD) in food contact papers (FCMs). PEI could provide an alkaline environment and then react with dopamine (DA) to produce copolymers by Michael addition and Schiff-base reactions. This copolymer has a strong fluorescence emission at 527 nm. We found that amino groups of DA and PEI could also react with 3-MCPD in an alkaline medium, which improved the morphology and fluorescence intensity of PDA-PEI copolymers. The fluorescence intensity of the polymers was linear but inversely proportional to the concentration of 3-MCPD in the range of 10.0–500.0 μg kg−1 and the detection limit was 2 μg kg−1. The standard addition method was used in FCMs to demonstrate the practical applicability and the spiked recoveries ranged from 99.8 to 110.3 %. Finally, the levels of 3-MCPD in different FCMs (n = 70) were determined by the proposed method. The detection frequencies ranged from 25 % to 100 % and both the highest detection frequency and levels were observed in kitchen papers. More than half of the samples did not comply with the limits recommended by the German Federal Institute for Risk Assessment, suggesting that 3-MCPD released from FCMs is a major route of human exposure.
摘要：
Degumming, a critical process in the edible oil industry, is essential for removing phospholipids. Traditional methods such as water-degumming and acid-degumming have limitations in this regard. This study aimed to assess the efficacy of different degumming techniques, including acid, single and multi-enzyme methods, on rice bran oil (RBO). The investigation also focused on the impact of these techniques on the physicochemical characteristics and preservation of micronutrients in RBO during the degumming process. The primary phospholipids identified were phosphatidylethanolamine (29.51 %), phosphatidylcholine (37.00 %), and phosphatidylinositol (24.49 %). Acid degumming removed 84.23 % of phospholipids, while a significantly higher removal rate of 98.7 % was achieved with the combination of phospholipase A1 & phospholipase C. The degumming process effectively inhibited oxidation in RBO, leading to a substantial increase in the oxidation induction time from 5.7 to 10.0 hours. Furthermore, multi-enzyme degumming showed slightly greater radical-scavenging activity compared to single enzyme degumming in RBO. However, the levels of micronutrients such as phenols, sterols, tocopherols, squalene, and oryzanol were reduced by 6.27–22.17 %. This study provides a comprehensive analysis of the effects of different degumming processes on the physicochemical properties, fatty acid profiles, antioxidant capacities, and preservation of micronutrients in RBO.
Degumming, a critical process in the edible oil industry, is essential for removing phospholipids. Traditional methods such as water-degumming and acid-degumming have limitations in this regard. This study aimed to assess the efficacy of different degumming techniques, including acid, single and multi-enzyme methods, on rice bran oil (RBO). The investigation also focused on the impact of these techniques on the physicochemical characteristics and preservation of micronutrients in RBO during the degumming process. The primary phospholipids identified were phosphatidylethanolamine (29.51 %), phosphatidylcholine (37.00 %), and phosphatidylinositol (24.49 %). Acid degumming removed 84.23 % of phospholipids, while a significantly higher removal rate of 98.7 % was achieved with the combination of phospholipase A1 & phospholipase C. The degumming process effectively inhibited oxidation in RBO, leading to a substantial increase in the oxidation induction time from 5.7 to 10.0 hours. Furthermore, multi-enzyme degumming showed slightly greater radical-scavenging activity compared to single enzyme degumming in RBO. However, the levels of micronutrients such as phenols, sterols, tocopherols, squalene, and oryzanol were reduced by 6.27–22.17 %. This study provides a comprehensive analysis of the effects of different degumming processes on the physicochemical properties, fatty acid profiles, antioxidant capacities, and preservation of micronutrients in RBO.
期刊：
International Journal of Biological Macromolecules,2025年293:139340 ISSN：0141-8130
通讯作者：
Zhang, Rui;He, JR
作者机构：
[Chen, Ming; Pei, Xun; Yin, Jinjing; Zhang, Rui; Xiong, Sihui; Wu, Muci; He, Jingren] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Oliveira, Helder; Mateus, Nuno] Univ Porto, Fac Sci, Dept Chem & Biochem, REQUIMTE LAQV, P-4169007 Porto, Portugal.;[Ye, Shuxin] Yun Hong Grp Co Ltd, Wuhan 430206, Hubei, Peoples R China.;[Zhang, Rui; He, Jingren] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[He, Jingren; Zhang, Rui] Wuhan Polytech Univ, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.
通讯机构：
[He, JR ; Zhang, R] W;Wuhan Polytech Univ, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.
关键词：
Antioxidant activity;Antiproliferative capacity;Phenolics;Protein;Purple rice (Oryza sativa L.)
摘要：
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
关键词：
Gel properties;Gelatinizing properties;Microstructure;Wheat starch;gWPI
摘要：
Glycated whey protein isolate (gWPI) was produced by dry thermal reaction between whey protein isolate (WPI) and lactose, and its influence on the gelatinization, gel properties, and microstructure changes of wheat starch (WS) was systematically studied. Results showed that gWPI significantly inhibited starch gelatinization and improved starch gel properties. RVA and DSC analysis showed that gWPI decreased viscosity and gelatinization enthalpy (ΔH) in a concentration-dependent manner. When gWPI concentration was 12 %, the peak viscosity and ΔH decreased by 36 cP and 3.11 J/g, respectively. gWPI competed with WS for water and inhibited WS water absorption and expansion. Rheological results showed that the viscoelasticity of the gel decreased by adding gWPI and that of WS-gWPI was a pseudoplastic fluid with shear-thinning behavior. In addition, gWPI covered the WS surface in the form of hydrogen bonds, which inhibited the leaching of amylose, thereby reducing the particle size of the gelatinized starch and its iodine binding ability. AFM results showed that gWPI combined with WS weakened the aggregation of starch molecular chains and decreased the height of starch. In conclusion, gWPI can be used as a gelatinizing regulator to improve starch properties.
Glycated whey protein isolate (gWPI) was produced by dry thermal reaction between whey protein isolate (WPI) and lactose, and its influence on the gelatinization, gel properties, and microstructure changes of wheat starch (WS) was systematically studied. Results showed that gWPI significantly inhibited starch gelatinization and improved starch gel properties. RVA and DSC analysis showed that gWPI decreased viscosity and gelatinization enthalpy (ΔH) in a concentration-dependent manner. When gWPI concentration was 12 %, the peak viscosity and ΔH decreased by 36 cP and 3.11 J/g, respectively. gWPI competed with WS for water and inhibited WS water absorption and expansion. Rheological results showed that the viscoelasticity of the gel decreased by adding gWPI and that of WS-gWPI was a pseudoplastic fluid with shear-thinning behavior. In addition, gWPI covered the WS surface in the form of hydrogen bonds, which inhibited the leaching of amylose, thereby reducing the particle size of the gelatinized starch and its iodine binding ability. AFM results showed that gWPI combined with WS weakened the aggregation of starch molecular chains and decreased the height of starch. In conclusion, gWPI can be used as a gelatinizing regulator to improve starch properties.
摘要：
Epidemiological evidence on maternal thyroid function disruption by prenatal exposure to perfluorinated and polyfluorinated substances (PFASs) is limited and inconsistent. The study examined the effects of PFASs exposure during early pregnancy on maternal thyroid function [free thyroxine (FT4), thyroid stimulating hormone (TSH), thyroid peroxidase antibodies (TPOAb) and FT4/TSH ratio]. The associations were evaluated using both single and mixed pollutant models, statistical analyses were further utilized in benchmark dose (BMD) estimations to offer critical references for human health risk assessment. Linear regression was used and then Bonferroni correction adjustment was set up to correct for multiple comparisons. The results revealed a significant association between PFHxS exposure and TSH (β = 0.473; 95% CI: 0.180, 0.767). According to BKMR mixed-effects models, PFHxS was significantly positively correlated with TSH at the 25th percentile. PFASs were associated with the FT4/TSH ratio at the 25th to 40th percentile. The BMD value of the increasing FT4 effect induced by PFBA and PFPeA in pregnant women were 6.68 ng/mL and 1.37 ng/mL, respectively. The BMDs were obtained for TSH in the case of PFBA (0.33 ng/mL), PFHxS (0.28 ng/mL). Although BMDL 10 is higher than observed for maternal TSH elevation in animal studies, both studies agree that thyroid homeostasis is the sensitive target. The fact that BMD results at this stage are lower than current exposure levels to PFHxS underscores the urgency of prioritizing endocrine end points in PFASs risk assessment.
Epidemiological evidence on maternal thyroid function disruption by prenatal exposure to perfluorinated and polyfluorinated substances (PFASs) is limited and inconsistent. The study examined the effects of PFASs exposure during early pregnancy on maternal thyroid function [free thyroxine (FT4), thyroid stimulating hormone (TSH), thyroid peroxidase antibodies (TPOAb) and FT4/TSH ratio]. The associations were evaluated using both single and mixed pollutant models, statistical analyses were further utilized in benchmark dose (BMD) estimations to offer critical references for human health risk assessment. Linear regression was used and then Bonferroni correction adjustment was set up to correct for multiple comparisons. The results revealed a significant association between PFHxS exposure and TSH (β = 0.473; 95% CI: 0.180, 0.767). According to BKMR mixed-effects models, PFHxS was significantly positively correlated with TSH at the 25th percentile. PFASs were associated with the FT4/TSH ratio at the 25th to 40th percentile. The BMD value of the increasing FT4 effect induced by PFBA and PFPeA in pregnant women were 6.68 ng/mL and 1.37 ng/mL, respectively. The BMDs were obtained for TSH in the case of PFBA (0.33 ng/mL), PFHxS (0.28 ng/mL). Although BMDL 10 is higher than observed for maternal TSH elevation in animal studies, both studies agree that thyroid homeostasis is the sensitive target. The fact that BMD results at this stage are lower than current exposure levels to PFHxS underscores the urgency of prioritizing endocrine end points in PFASs risk assessment.
期刊：
Journal of Cereal Science,2025年:104196 ISSN：0733-5210
通讯作者：
Bin Tan
作者机构：
[Xiaoning Li; Bin Tan; Liping Wang] Institute of Cereal Processing Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China;School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[Qian Xu] Institute of Cereal Processing Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China<&wdkj&>School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
通讯机构：
[Bin Tan] I;Institute of Cereal Processing Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China
摘要：
The individual and combined effects of cold plasma and enzymatic hydrolysis modification on the yield, structural, physicochemical and functional properties of soluble dietary fiber (SDF) derived from wheat bran were investigated. The results indicated that enzymatic hydrolysis and combined modification (cold plasma followed by enzymatic hydrolysis) significantly enhanced the yield of SDF to 15.08% and 14.65%, respectively. In terms of structure, all three modifications resulted in partial cleavage of glycosidic and hydrogen bonds, leading to the reduction in the molecular weights of SDFs. The cold plasma-modified SDF displayed a loose lamellar microstructure with small pores, while honeycomb-like pores were observed on the surface of SDF modified by two others. The combined-modified SDF exhibited the most significant structural alterations, which resulted in its lowest viscosity and highest water solubility in all tested SDF. Furthermore, the cold plasma-modified SDF demonstrated the highest α-amylase inhibition ability and bile salt adsorption capacity; while the combined-modified SDF showed the best performance in glucose adsorption capacity, cholesterol adsorption capacity and antioxidant capacity. In conclusion, the combination of cold plasma and enzymatic hydrolysis is a promising strategy for improving not only the yield but also the physicochemical and functional properties of SDF in wheat bran.
The individual and combined effects of cold plasma and enzymatic hydrolysis modification on the yield, structural, physicochemical and functional properties of soluble dietary fiber (SDF) derived from wheat bran were investigated. The results indicated that enzymatic hydrolysis and combined modification (cold plasma followed by enzymatic hydrolysis) significantly enhanced the yield of SDF to 15.08% and 14.65%, respectively. In terms of structure, all three modifications resulted in partial cleavage of glycosidic and hydrogen bonds, leading to the reduction in the molecular weights of SDFs. The cold plasma-modified SDF displayed a loose lamellar microstructure with small pores, while honeycomb-like pores were observed on the surface of SDF modified by two others. The combined-modified SDF exhibited the most significant structural alterations, which resulted in its lowest viscosity and highest water solubility in all tested SDF. Furthermore, the cold plasma-modified SDF demonstrated the highest α-amylase inhibition ability and bile salt adsorption capacity; while the combined-modified SDF showed the best performance in glucose adsorption capacity, cholesterol adsorption capacity and antioxidant capacity. In conclusion, the combination of cold plasma and enzymatic hydrolysis is a promising strategy for improving not only the yield but also the physicochemical and functional properties of SDF in wheat bran.
摘要：
Acid-catalyzed organosolv pretreatments using various acids and organic solvents have been widely studied for biomass fractionation. However, few studies have explored whether specific combinations of acids and solvents are necessary to achieve optimal enzymatic cellulose hydrolysis. In this study, organosolv pretreatments were performed on corn stover under mild conditions (120 °C, 2 h) using four biomass-derived solvents (ethylene glycol (EG), 1,4-butanediol (BDO), dimethyl isosorbide (DMI), and γ-valerolactone (GVL)) in an 80:20 solvent-to-water weight ratio, combined with four acids (HCl, H 2 SO 4 , AlCl 3 and p-toluenesulfonic acid (TsOH)) as catalysts (0.1 mol/L). The results showed specific interactions between the acid and solvent. HCl- and AlCl 3 -catalyzed GVL/H 2 O, HCl- and TsOH-catalyzed EG/H 2 O, and HCl-catalyzed DMI/H 2 O exhibited high pretreatment efficacy, achieving enzymatic glucose yields of approximately 80 % after 48 h of hydrolysis. The evaluation of solvent effects using Hansen Solubility Parameters (HSP) revealed no clear correlation with delignification, likely due to the influence of acidic catalysts and the formation of condensed lignin and pseudo-lignin, which may distort the delignification data. Additionally, the chemical composition and cellulose-related factors (accessibility, degree of polymerization and crystallinity) of pretreated biomass were analyzed and correlated with enzymatic glucose yield to evaluate their effects on biomass saccharification. In summary, this study underscores the specificity of acids in organosolv biomass pretreatment and cautions against relying solely on HSP theory for solvent selection when using acidic catalysts.
Acid-catalyzed organosolv pretreatments using various acids and organic solvents have been widely studied for biomass fractionation. However, few studies have explored whether specific combinations of acids and solvents are necessary to achieve optimal enzymatic cellulose hydrolysis. In this study, organosolv pretreatments were performed on corn stover under mild conditions (120 °C, 2 h) using four biomass-derived solvents (ethylene glycol (EG), 1,4-butanediol (BDO), dimethyl isosorbide (DMI), and γ-valerolactone (GVL)) in an 80:20 solvent-to-water weight ratio, combined with four acids (HCl, H 2 SO 4 , AlCl 3 and p-toluenesulfonic acid (TsOH)) as catalysts (0.1 mol/L). The results showed specific interactions between the acid and solvent. HCl- and AlCl 3 -catalyzed GVL/H 2 O, HCl- and TsOH-catalyzed EG/H 2 O, and HCl-catalyzed DMI/H 2 O exhibited high pretreatment efficacy, achieving enzymatic glucose yields of approximately 80 % after 48 h of hydrolysis. The evaluation of solvent effects using Hansen Solubility Parameters (HSP) revealed no clear correlation with delignification, likely due to the influence of acidic catalysts and the formation of condensed lignin and pseudo-lignin, which may distort the delignification data. Additionally, the chemical composition and cellulose-related factors (accessibility, degree of polymerization and crystallinity) of pretreated biomass were analyzed and correlated with enzymatic glucose yield to evaluate their effects on biomass saccharification. In summary, this study underscores the specificity of acids in organosolv biomass pretreatment and cautions against relying solely on HSP theory for solvent selection when using acidic catalysts.
摘要：
Lotus root is a widely popular aquatic vegetable with edible and medicinal values. Here we report the structure and lipid-lowering activity of two lotus root polysaccharides LRW (lotus root polysaccharide by water extraction) and LRA (lotus root polysaccharide by alkali extraction), that were extracted by aqueous and alkaline solution respectively. The results showed that the yield of polysaccharide from lotus root could be significantly improved by alkali extraction. Basic composition and structural characterization showed that the total sugar contents of LRW and LRA were 96.83 % and 73.66 %, and the molecular weights were 2.464 × 10 5 Da and 1.727 × 10 5 Da, respectively. LRW and LRA had the similar structure that the main backbone consisted of →4)-α-D-Glcp-(1→ with branches at C-6 site. Both LRW and LRA could scavenge DPPH and hydroxyl radicals effectively, and have strong adsorption capacity to cholate salts in a concentration-dependent manner. In HepG2 cells, LRW and LRA inhibited the accumulation of lipid droplets induced by oleic acid, and increased the activity of T-SOD and CAT, meanwhile, reduced the level of MDA, TC and TG, showing good lipid-lowering activity. In comparison, the lipid-lowering effect of LRA was better than that of LRW. In addition, gene sequencing and RT-PCR showed that AMPK, ACC, PPARα and CPT-1 were essential for LRA to exert a lipid-lowering effect. This study provides a theoretical basis for the extraction and lipid-lowering application of lotus root polysaccharides.
Lotus root is a widely popular aquatic vegetable with edible and medicinal values. Here we report the structure and lipid-lowering activity of two lotus root polysaccharides LRW (lotus root polysaccharide by water extraction) and LRA (lotus root polysaccharide by alkali extraction), that were extracted by aqueous and alkaline solution respectively. The results showed that the yield of polysaccharide from lotus root could be significantly improved by alkali extraction. Basic composition and structural characterization showed that the total sugar contents of LRW and LRA were 96.83 % and 73.66 %, and the molecular weights were 2.464 × 10 5 Da and 1.727 × 10 5 Da, respectively. LRW and LRA had the similar structure that the main backbone consisted of →4)-α-D-Glcp-(1→ with branches at C-6 site. Both LRW and LRA could scavenge DPPH and hydroxyl radicals effectively, and have strong adsorption capacity to cholate salts in a concentration-dependent manner. In HepG2 cells, LRW and LRA inhibited the accumulation of lipid droplets induced by oleic acid, and increased the activity of T-SOD and CAT, meanwhile, reduced the level of MDA, TC and TG, showing good lipid-lowering activity. In comparison, the lipid-lowering effect of LRA was better than that of LRW. In addition, gene sequencing and RT-PCR showed that AMPK, ACC, PPARα and CPT-1 were essential for LRA to exert a lipid-lowering effect. This study provides a theoretical basis for the extraction and lipid-lowering application of lotus root polysaccharides.
摘要：
Tyrosol is an important component of pharmaceuticals, nutraceuticals, and cosmetics, and their biosynthetic pathways are currently a hot research topic. d -Erythrose 4-phosphate is a key precursor for the biosynthesis of tyrosol in Saccharomyces cerevisiae . Hence, the flux of d -Erythrose 4-phosphate determined the yield of tyrosol synthesis. In this study, we first obtained an S. cerevisiae strain S19 with a tyrosol yield of 247.66 mg/L by metabolic engineering strategy. To increase the production of d -Erythrose 4-phosphate, highly active phosphoketolase BA-C was obtained by bioinformatics combined with tyrosol yield assay. The key residue sites 183, 217, and 320 were obtained by molecular docking, kinetic simulation, and tyrosol yield verification. After mutation, the highly efficient phosphoketolase BA-C His320Met was obtained, with a 37.32 % increase in enzyme activity. The tyrosol production of strain S26 with BA-C His320Arg increased by 43.05 % than strain S25 with BA-C and increased by 151.19 % compared with the strain S19 without phosphoketolase in a 20 L fermenter. The mining and modification of phosphoketolase will provide strong support for the de novo synthesis of aromatic compounds.
Tyrosol is an important component of pharmaceuticals, nutraceuticals, and cosmetics, and their biosynthetic pathways are currently a hot research topic. d -Erythrose 4-phosphate is a key precursor for the biosynthesis of tyrosol in Saccharomyces cerevisiae . Hence, the flux of d -Erythrose 4-phosphate determined the yield of tyrosol synthesis. In this study, we first obtained an S. cerevisiae strain S19 with a tyrosol yield of 247.66 mg/L by metabolic engineering strategy. To increase the production of d -Erythrose 4-phosphate, highly active phosphoketolase BA-C was obtained by bioinformatics combined with tyrosol yield assay. The key residue sites 183, 217, and 320 were obtained by molecular docking, kinetic simulation, and tyrosol yield verification. After mutation, the highly efficient phosphoketolase BA-C His320Met was obtained, with a 37.32 % increase in enzyme activity. The tyrosol production of strain S26 with BA-C His320Arg increased by 43.05 % than strain S25 with BA-C and increased by 151.19 % compared with the strain S19 without phosphoketolase in a 20 L fermenter. The mining and modification of phosphoketolase will provide strong support for the de novo synthesis of aromatic compounds.
作者机构：
[Xiaoxue Yin; Fangjiao Chen; Bingyu Han; Xuedong Wang; Wenping Ding; Beibei Ding] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[Jun You] Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key laboratory of Polymer Materials, School of Materials Science and Engineering, Hubei University, Wuhan, 430062, China
通讯机构：
[Beibei Ding] S;School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
摘要：
Pickering emulsions have been acknowledged as a viable method for safeguarding the flavor compounds in essential oils. In this study, chitin nanowhiskers (ChNWs) was prepared via ultrasonic exfoliation of chitin that had undergone pretreatment involving ammonium persulfate (APS) oxidation and partial deacetylation. The prepared ChNWs were then employed to stabilize Pickering emulsions for the encapsulation of flavor compounds in Zanthoxylum bungeanum oil (ZBO). The stability of the Pickering emulsions was assessed through various techniques including EI value, particle size distribution, CLSM, rheological properties, and the release of flavor compounds was analyzed using electronic nose and GC-IMS. The findings indicated that the ChNWs strongly adsorbed at the oil-water interface, leading to the formation of a protective capping layer around the ZBO droplets. This phenomenon effectively reduced the rate of lipid oxidation and inhibited the release of flavor compounds.
Pickering emulsions have been acknowledged as a viable method for safeguarding the flavor compounds in essential oils. In this study, chitin nanowhiskers (ChNWs) was prepared via ultrasonic exfoliation of chitin that had undergone pretreatment involving ammonium persulfate (APS) oxidation and partial deacetylation. The prepared ChNWs were then employed to stabilize Pickering emulsions for the encapsulation of flavor compounds in Zanthoxylum bungeanum oil (ZBO). The stability of the Pickering emulsions was assessed through various techniques including EI value, particle size distribution, CLSM, rheological properties, and the release of flavor compounds was analyzed using electronic nose and GC-IMS. The findings indicated that the ChNWs strongly adsorbed at the oil-water interface, leading to the formation of a protective capping layer around the ZBO droplets. This phenomenon effectively reduced the rate of lipid oxidation and inhibited the release of flavor compounds.
摘要：
The effects of different phenolic compounds (Epigallocatechin gallate, EGCG; Catechin, CC; Tannic acid, TA) at different phenol hydroxyl concentrations on the gel strength, water holding capacity (WHC), water distribution, and micro-morphology of surimi gels were investigated. Breaking force and deformation of surimi gels treated with EGCG, CC, and TA were increased by 20%, 15%, 12% and 14%, 7%, 4%, compared with untreated surimi gel. Polyphenols increased the WHC of surimi gels and induced the conversion of α-helix to β-sheet in proteins, which was beneficial to the gelation of surimi gel. Surimi gels treated with polyphenols had a complete three-dimensional network structure and relatively uniform and small pores, especially EGCG cross-linked surimi gel. Molecular docking indicated that the interactions between EGCG, CC, TA and myosin heavy chain II A were mainly hydrogen bond and hydrophobic interaction, and their binding energies were −37, −31, and −26 kJ/mol. The binding energy of polyphenols with myosin heavy chain II A correlates with their cross-linking ability with proteins, and the lower the binding energy is, the stronger the cross-linking ability is. This study provides important information to elucidate the mechanism of phenolic compounds regulating the physical properties of surimi gel.
The effects of different phenolic compounds (Epigallocatechin gallate, EGCG; Catechin, CC; Tannic acid, TA) at different phenol hydroxyl concentrations on the gel strength, water holding capacity (WHC), water distribution, and micro-morphology of surimi gels were investigated. Breaking force and deformation of surimi gels treated with EGCG, CC, and TA were increased by 20%, 15%, 12% and 14%, 7%, 4%, compared with untreated surimi gel. Polyphenols increased the WHC of surimi gels and induced the conversion of α-helix to β-sheet in proteins, which was beneficial to the gelation of surimi gel. Surimi gels treated with polyphenols had a complete three-dimensional network structure and relatively uniform and small pores, especially EGCG cross-linked surimi gel. Molecular docking indicated that the interactions between EGCG, CC, TA and myosin heavy chain II A were mainly hydrogen bond and hydrophobic interaction, and their binding energies were −37, −31, and −26 kJ/mol. The binding energy of polyphenols with myosin heavy chain II A correlates with their cross-linking ability with proteins, and the lower the binding energy is, the stronger the cross-linking ability is. This study provides important information to elucidate the mechanism of phenolic compounds regulating the physical properties of surimi gel.
摘要：
Fractionation allows the separation of components in beef tallow. This study compared the physicochemical characteristics and cholesterol content of beef tallow and its liquid fraction, evaluating their frying performance as potential deep-fat frying oils against plant oils. Results showed effective separation of unsaturated components from beef tallow through fractionation. Beef tallow exhibited superior physicochemical properties during frying, with lower deterioration levels than plant oils. Benzo[ a ]pyrene content increased in plant oils but remained low in beef tallow and its liquid fraction. The liquid fraction had a significantly shorter oxidative induction time of 0.38 h compared to 5.85 h and 5.24 h for plant oils. This study revealed that alterations were observed in beef tallow and its liquid fraction when used as frying oils, with beef tallow demonstrating stronger antioxidative properties compared to the liquid fraction, which exhibited lower levels of cholesterol and saturated fatty acids.
Fractionation allows the separation of components in beef tallow. This study compared the physicochemical characteristics and cholesterol content of beef tallow and its liquid fraction, evaluating their frying performance as potential deep-fat frying oils against plant oils. Results showed effective separation of unsaturated components from beef tallow through fractionation. Beef tallow exhibited superior physicochemical properties during frying, with lower deterioration levels than plant oils. Benzo[ a ]pyrene content increased in plant oils but remained low in beef tallow and its liquid fraction. The liquid fraction had a significantly shorter oxidative induction time of 0.38 h compared to 5.85 h and 5.24 h for plant oils. This study revealed that alterations were observed in beef tallow and its liquid fraction when used as frying oils, with beef tallow demonstrating stronger antioxidative properties compared to the liquid fraction, which exhibited lower levels of cholesterol and saturated fatty acids.
作者机构：
[Xiaorui Zhang; Xingyi Huang; Xiaoyu Tian; Chengquan Wang; Shanshan Yu; Joshua Harrington Aheto] School of Food and Biological Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, Jiangsu, PR China;[Chunxia Dai] School of Electrical and Information Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, Jiangsu, PR China;[Yi Ren] School of Smart Agriculture, Suzhou Polytechnic Institute of Agriculture, Xiyuan Road 279, Suzhou 215008, Jiangsu, PR China;[Li Wang] Culinary Science and Technology Department, Jiangsu College of Tourism, Yangzhou 225000, PR China;[Xianhui Chang] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, Hubei, PR China
通讯机构：
[Xingyi Huang] S;School of Food and Biological Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, Jiangsu, PR China
摘要：
To elucidate the volatile flavor compositions and overall aroma profiles of fermented bean curd (FBC), the volatile organic compounds (VOCs) in six different FBC were detected and characterized using gas chromatography-ion mobility spectrometry (GC-IMS) and an electronic nose (E-nose) in this study. A total of 60 VOCs were identified by GC-IMS, of which esters, aldehydes, alcohols, and ketones constituted the major compounds. Among them, 17 VOCs were identified as key differentiating volatile compounds. In addition, the E-nose combined two algorithms, Linear Discriminant Analysis (LDA) and K-Nearest Neighbor (KNN), to demonstrate its effectiveness in differentiating between different FBC samples. The results showed that the LDA model performed better than the KNN model. When the principal component number was 9, the recognition accuracies of the training and prediction sets for the LDA model were 94.44% and 91.67%, respectively. In addition, a multi-channel colorimetric sensor array (CSA) was constructed in this study for the quantitative prediction of key physicochemical indicators. The results showed that both partial least squares regression (PLSR) and support vector machine regression (SVR) achieved good prediction performance. Among them, for the SVR model, the prediction correlation coefficients for total acidity, reducing sugar, salinity, and amino acid nitrogen were 0.9033, 0.9170, 0.7298, and 0.9213, respectively. The results of this study indicate that GC-IMS, E-nose, and CSA are expected to be effective tools for characterizing FBC flavor as well as facilitating the rapid quantification of key physicochemical indicators, which may provide valuable insights for flavor and quality control in traditional fermented foods.
To elucidate the volatile flavor compositions and overall aroma profiles of fermented bean curd (FBC), the volatile organic compounds (VOCs) in six different FBC were detected and characterized using gas chromatography-ion mobility spectrometry (GC-IMS) and an electronic nose (E-nose) in this study. A total of 60 VOCs were identified by GC-IMS, of which esters, aldehydes, alcohols, and ketones constituted the major compounds. Among them, 17 VOCs were identified as key differentiating volatile compounds. In addition, the E-nose combined two algorithms, Linear Discriminant Analysis (LDA) and K-Nearest Neighbor (KNN), to demonstrate its effectiveness in differentiating between different FBC samples. The results showed that the LDA model performed better than the KNN model. When the principal component number was 9, the recognition accuracies of the training and prediction sets for the LDA model were 94.44% and 91.67%, respectively. In addition, a multi-channel colorimetric sensor array (CSA) was constructed in this study for the quantitative prediction of key physicochemical indicators. The results showed that both partial least squares regression (PLSR) and support vector machine regression (SVR) achieved good prediction performance. Among them, for the SVR model, the prediction correlation coefficients for total acidity, reducing sugar, salinity, and amino acid nitrogen were 0.9033, 0.9170, 0.7298, and 0.9213, respectively. The results of this study indicate that GC-IMS, E-nose, and CSA are expected to be effective tools for characterizing FBC flavor as well as facilitating the rapid quantification of key physicochemical indicators, which may provide valuable insights for flavor and quality control in traditional fermented foods.
期刊：
International Journal of Biological Macromolecules,2025年308(Pt 3):142352 ISSN：0141-8130
通讯作者：
Cai, J
作者机构：
[Cai, Jie; Liu, Nian; Yang, Zhaoxing; He, Zhijun; Feng, Xiaofang; Xie, Fang] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Liu, Xiaoqing] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Delivery;OSA starch;Selenium nanoparticles
摘要：
Selenium nanoparticles (SeNPs) exhibit significant potential in antitumor therapy. However, challenges such as aggregation and lack of targeting capability limit their application. Herein, we developed selenium-loaded octenyl succinic anhydride starch (OSAS) micelles functionalized with folic acid (FA) for targeted tumor delivery. The FA-OSAS-SeNPs were synthesized through self-assembly, incorporating SeNPs into FA-conjugated OSAS micelles. Fourier Transform Infrared (FTIR) spectroscopy and UV–visible spectrophotometry confirmed the successful synthesis of FA-OSAS-SeNPs. The nanoparticles exhibited an average size of 131.66 ± 7.88 nm and a zeta potential of −19.54 ± 0.33 mV, with encapsulation efficiency and drug loading capacity of approximately 87.28 % and 8.96 %, respectively. FA-OSAS-SeNPs demonstrated good stability across various conditions, including different dilution ratios, temperatures, pH levels, and ionic strengths. In vitro studies showed that FA-OSAS-SeNPs exhibited significant targeted inhibitory effects on cervical cancer (HeLa) cells and markedly increased intracellular ROS levels, inducing apoptosis. This study presents a novel and effective strategy for targeted SeNPs delivery systems in tumor therapy, offering a valuable reference for future development of nanomaterials for clinical applications in cancer treatment.
Selenium nanoparticles (SeNPs) exhibit significant potential in antitumor therapy. However, challenges such as aggregation and lack of targeting capability limit their application. Herein, we developed selenium-loaded octenyl succinic anhydride starch (OSAS) micelles functionalized with folic acid (FA) for targeted tumor delivery. The FA-OSAS-SeNPs were synthesized through self-assembly, incorporating SeNPs into FA-conjugated OSAS micelles. Fourier Transform Infrared (FTIR) spectroscopy and UV–visible spectrophotometry confirmed the successful synthesis of FA-OSAS-SeNPs. The nanoparticles exhibited an average size of 131.66 ± 7.88 nm and a zeta potential of −19.54 ± 0.33 mV, with encapsulation efficiency and drug loading capacity of approximately 87.28 % and 8.96 %, respectively. FA-OSAS-SeNPs demonstrated good stability across various conditions, including different dilution ratios, temperatures, pH levels, and ionic strengths. In vitro studies showed that FA-OSAS-SeNPs exhibited significant targeted inhibitory effects on cervical cancer (HeLa) cells and markedly increased intracellular ROS levels, inducing apoptosis. This study presents a novel and effective strategy for targeted SeNPs delivery systems in tumor therapy, offering a valuable reference for future development of nanomaterials for clinical applications in cancer treatment.
作者机构：
[Shen, Wangyang; Jia, Xiwu; Luo, Xiaohua; Jin, Weiping; Liu, Xin] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China;[Shen, Wangyang; Jia, Xiwu; Jin, Weiping] Key Laboratory of the Deep Processing of Bulk Grain and Oil Authorized by Ministry of Education, Wuhan 430000, Hubei, PR China;[Wu, Yongning] Department of Nutrition and Food Safety, Peking Union Medical College, Research Unit of Food Safety, Chinese Academy of Medical Sciences, Beijing 100021, China;[Wu, Yongning] NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100021, China;[Liu, Xin] Key Laboratory of the Deep Processing of Bulk Grain and Oil Authorized by Ministry of Education, Wuhan 430000, Hubei, PR China. Electronic address: liuxinhook@whpu.edu.cn
通讯机构：
[Xin Liu] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China<&wdkj&>Key Laboratory of the Deep Processing of Bulk Grain and Oil Authorized by Ministry of Education, Wuhan 430000, Hubei, PR China
摘要：
The effect of Chlorella pyrenoidosa (CP) and Spirulina platensis (SP) at concentrations of 0 %–12 % on the properties of rice starch (RS) was investigated. Compared with pure RS, the addition of CP and SP powder decreased the viscosity, increased the gelatinization temperature, and promoted the retrogradation of RS gel. However, when CP was added at 12 % and SP at 8 %, retrogradation inhibition was reduced. At these concentrations, the relative crystallinity of the CP mixture increased by 57.37 %, whereas that of SP increased by 48.13 %. Scanning electron microscopy revealed that the addition of low amount of CP and SP reduced porosity. CP and SP powder facilitated the conversion of bound water to free water and contributed to the weakening of the viscoelasticity of the RS gel. CP powder likely had a more detrimental effect on the short-term storage properties of RS than SP powder. These results provide theoretical support for the development of RS-based products and the innovative utilization of microalgae.
The effect of Chlorella pyrenoidosa (CP) and Spirulina platensis (SP) at concentrations of 0 %–12 % on the properties of rice starch (RS) was investigated. Compared with pure RS, the addition of CP and SP powder decreased the viscosity, increased the gelatinization temperature, and promoted the retrogradation of RS gel. However, when CP was added at 12 % and SP at 8 %, retrogradation inhibition was reduced. At these concentrations, the relative crystallinity of the CP mixture increased by 57.37 %, whereas that of SP increased by 48.13 %. Scanning electron microscopy revealed that the addition of low amount of CP and SP reduced porosity. CP and SP powder facilitated the conversion of bound water to free water and contributed to the weakening of the viscoelasticity of the RS gel. CP powder likely had a more detrimental effect on the short-term storage properties of RS than SP powder. These results provide theoretical support for the development of RS-based products and the innovative utilization of microalgae.
