2,4-dinitrotoluene (Dnt) perturbs yolk absorption, liver development and lipid metabolism/oxygen transport gene expression in zebrafish embryos and larvae
作者：
Xiong, Jianglin;Sha, Hang;Zhou, Hualin;Peng, Lijuan;Wu, Lingying;...
期刊：
International Journal of Molecular Sciences ,2019年20(15):3632 ISSN：1661-6596
通讯作者：
Wang, Rui
作者机构：
[Hu, Xianqin; Wu, Lingying; Xiong, Jianglin; Qiu, Yinsheng; Wang, Rui] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Sha, Hang] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China.;[Zhou, Hualin] Xiangyang Vocat & Tech Coll, Agr Coll, Xiangyang 441050, Peoples R China.;[Peng, Lijuan] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Wang, Rui] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
2,4 dinitrotoluene;acyl coenzyme A oxidase;apolipoprotein A2;fatty acid binding protein;green fluorescent protein;heme oxygenase;hypoxia inducible factor 1alpha;microsomal triglyceride transfer protein;peroxisome proliferator activated receptor alpha;peroxisome proliferator activated receptor gamma;transferrin;2,4-dinitrotoluene;nitrobenzene derivative;oxygen;adult;animal experiment;animal model;animal tissue;Article;breathing;breeding;controlled study;down regulation;embryo;embryo development;fish breeding;gene expression;genetic transcription;larva;LC50;lipid metabolism;lipid transport;lipolysis;liver development;medical parameters;morphometry;mortality;nonhuman;oxygen transportation;polymerase chain reaction;post fertilization;real time polymerase chain reaction;reverse transcription polymerase chain reaction;RNA isolation;stereomicroscopy;upregulation;yolk absorption;zebra fish;animal;drug effect;embryology;gene expression regulation;larva;lipid metabolism;liver;metabolism;nonmammalian embryo;organogenesis;pharmacology;pollutant;toxicity;transport at the cellular level;Animals;Biological Transport;Dinitrobenzenes;Embryo, Nonmammalian;Embryonic Development;Environmental Pollutants;Gene Expression Regulation, Developmental;Larva;Lipid Metabolism;Lipolysis;Liver;Organogenesis;Oxygen;Zebrafish
摘要：
2,4-dinitrotoluene (2,4-DNT) is a common environmental pollutant, and was classified as a group 2B human carcinogenic compound by the International Agency for Research on Cancer. This study determined the toxic effects of 2,4-DNT exposure on zebrafish at the embryo-larvae stage, in terms of organ morphogenesis and the expression pattern of selected target genes related to lipid metabolism and oxygen transportation. The results showed that the 120-h post-fertilization LC50 of 2,4-DNT was 9.59 mg/L with a 95% confidence interval of 8.89–10.44 mg/L. The larvae treated with 2,4-DNT showed toxic symptoms including smaller body, less skin pigment production, yolk malabsorption, and disordered liver development. Further studies on the expression of genes related to lipid transport and metabolism, and respiration indicated that they were significantly affected by 2,4-DNT. It is concluded that 2,4-DNT exposure perturbed liver development and yolk absorption in early-life zebrafish, and disturbed the lipid metabolism /oxygen transport gene expression. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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英文
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Yeast cell walls stimulate viability, respiratory burst, and phagocytosis in channel catfish (Ictalurus punctatus) head-kidney macrophages
作者：
Zhou, Jianjun;Gao, Zhen;Wang, Wenbiao;Huang, Feng;Hu, Junpeng;...
期刊：
Aquaculture International ,2019年27(6):1655-1665 ISSN：0967-6120
通讯作者：
Hu, Xianqin;Wang, Xuedong
作者机构：
[Hu, Xianqin; Hu, XQ; Wang, XD; Gao, Zhen; Huang, Feng; Wang, Wenbiao; Yang, Wumin; Zhou, Jianjun; Wang, Rui; Wang, Xuedong] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn,Hubei Key Lab Anim Nutr, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Engn Res Ctr Feed Prot Resources Agr By Prod,Mini, Wuhan 430023, Hubei, Peoples R China.;[Gong, Aqiong; Hu, Junpeng] Angel Yeast Co Ltd, Yichang City 443003, Hubei, Peoples R China.;[Li, Jie] Dong Xihu Farm Adm Hubei Prov, Agr Comprehens Serv Stn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Hu, XQ; Wang, XD] W;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn,Hubei Key Lab Anim Nutr, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Engn Res Ctr Feed Prot Resources Agr By Prod,Mini, Wuhan 430023, Hubei, Peoples R China.
关键词：
Yeast cell wall;Channel catfish;Head-kidney macrophage;Respiratory burst;Phagocytosis
摘要：
Yeast cell wall (YCW) preparations are important nutritional additives in the aquaculture industry. Head-kidney macrophages play important roles in innate immune functions in fish. However, the mechanisms underlying their stimulation are not yet characterized. The purpose of this study was to investigate the effects of YCW on immunological functions of channel catfish (Ictalurus punctatus) head-kidney macrophages. Our results showed that YCW preparations not only improved the proliferation and phagocytosis of macrophages but also induced the secretion of reactive oxygen species (ROS), nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta). At a concentration of 500.0 mu g/mL, YCW preparations stimulated macrophage proliferation and phagocytosis to 1.98 and 1.8 times, respectively, when compared with the negative group. Equally, YCW preparations raised the production of NO by inducing iNOS and ROS in a concentration-dependent manner. Furthermore, these preparations enhanced TNF-alpha and IL-1 beta mRNA levels to multiple digits from 1.2 to 3.0 times, respectively. In conclusion, the stimulatory effects of YCW on the phagocytic activity of the fish macrophages were associated with respiratory bursts coupled with nitric oxide production.
语种：
英文
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Sicuophora (Syn. Wichtermania) multigranularis from Quasipaa spinosa (Anura): Morphological and molecular study, with emphasis on validity of Sicuophora (Armophorea, Clevelandellida)
作者：
Li, Can;Zhao, Weishan;Zhang, Dong;Wang, Runqiu;Wang, Guitang;...
期刊：
PARASITE ,2018年25:38 ISSN：1252-607X
通讯作者：
Li, Ming
作者机构：
[Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Can; Li, Wenxiang] Chinese Acad Sci, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China.;[Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Can; Li, Wenxiang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.;[Li, Can] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Li, Ming] C;Chinese Acad Sci, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China.;Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
关键词：
animal;Anura;ciliate;classification;genetics;isolation and purification;parasitology;phylogeny;RNA gene;scanning electron microscopy;species difference;ultrastructure;Animals;Anura;Ciliophora;Genes, rRNA;Microscopy, Electron, Scanning;Phylogeny;Species Specificity
摘要：
Morphological studies of Sicuophora (Syn. Wichtermania) multigranularis Xiao et al., 2002, from the rectum of the frog, Quasipaa spinosa, performed using silver impregnation and scanning electron microscopy, confirmed the following newly recognized features: (1) only one apical suture on the right surface; (2) two naked regions at the posterior end of both the left and the right side of the body. Phylogenetic analysis based on the SSU-rRNA gene showed that S. multigranularis is a sister to a clade comprising all other Clevelandellida, strongly supporting the validity of the genus Sicuophora. This is also the first molecular data obtained for the genus Sicuophora. Because of the lack of molecular data, it will be necessary to obtain more genetic data from the family Sicuophoridae to discuss the question of the taxonomic status of the genus Sicuophora. SSU-rRNA. © 2018 EDP Sciences.
语种：
英文
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Balantidium grimi n. sp. (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China
作者：
Zhao, Weishan;Li, Can;Zhang, Dong;Wang, Runqiu;Zheng, Yingzhen;...
期刊：
PARASITE ,2018年25:29 ISSN：1252-607X
通讯作者：
Li, Ming
作者机构：
[Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Wenxiang] Chinese Acad Sci, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Hubei, Peoples R China.;[Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Wenxiang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.;[Li, Can] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Dong; Wang, Runqiu; Zhao, Weishan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Zheng, Yingzhen] Agr Forestry Anim Husb & Aquaculture Bur Guye Dis, Anim Husb & Aquaculture Stn, Tangshan 063100, Peoples R China.
通讯机构：
[Li, Ming] C;Chinese Acad Sci, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Hubei, Peoples R China.;Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
关键词：
protozoal DNA;ribosome DNA;anatomy and histology;animal;Anura;Balantidium;China;classification;genetics;isolation and purification;microscopy;parasitology;phylogeny;rectum;scanning electron microscopy;ultrastructure;Animals;Anura;Balantidium;China;DNA, Protozoan;DNA, Ribosomal;Microscopy;Microscopy, Electron, Scanning;Phylogeny;Rectum
摘要：
Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is "V"-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.
语种：
英文
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核苷酸对斑点叉尾鮰幼鱼生长、体成分和胃肠道形态的影响
作者：
沙航;王锐;祝爱侠;王春维
期刊：
粮食与饲料工业 ,2015年(02):44-48 ISSN：1003-6202
作者机构：
武汉轻工大学//动物营养与饲料安全湖北省协同创新中心,湖北武汉,430023;[祝爱侠; 王锐; 沙航; 王春维] 武汉轻工大学
关键词：
核苷酸;生长;体成分;胃肠道形态;斑点叉尾鮰
摘要：
研究外源核苷酸——5’-腺苷酸、5’-尿苷酸二钠、5’-胞苷酸二钠、5’-鸟苷酸二钠和5’-肌苷酸二钠对斑点叉尾鮰幼鱼生长性能、体成分和胃肠道形态的影响。结果表明:基础饲料中添加外源核苷酸没有显著提高斑点叉尾鮰幼鱼的增重率和特定生长率,但显著改善了鱼体的干物质和粗蛋白质含量,同时对胃肠道形态的发育具有显著地促进作用,其中以0.10%核苷酸混合物添加水平较佳。
语种：
中文
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不同品种鸡蛋蛋黄的营养效果研究
作者：
沙航;胡素琴;王锐
期刊：
中国家禽 ,2015年37(03):51-53 ISSN：1004-6364
作者机构：
武汉轻工大学,动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[胡素琴; 王锐; 沙航] 武汉轻工大学
关键词：
蛋黄;营养效果;生长性能
摘要：
本研究旨在探索不同品种鸡蛋蛋黄的营养效果,首先测定了3种市售鸡蛋蛋黄的营养成分,进而通过高效液相色谱和质谱法分析了其脂肪酸含量,最后以斑马鱼幼鱼为受试生物,分析了3种蛋黄的饲喂效果.结果表明,不同品种鸡蛋蛋黄的粗蛋白含量不同,蛋黄3的粗蛋白含量显著高于试验蛋黄1(P＜0.05),而各组蛋黄的粗脂肪含量差异不显著,高效液相色谱和质谱分析显示其脂肪酸含量存在差异,蛋黄3的二十碳以上不饱和脂肪酸含量较高;通过饲喂斑马鱼幼鱼显示其营养效果存在差异,与蛋黄1、2相比,蛋黄3能显著提高斑马鱼幼鱼的生长性能(P＜0.05).总之,不同品种鸡蛋蛋黄的蛋白质含量和不饱和脂肪酸含量存在差异,这直接影响了蛋黄的营养效果.
语种：
中文
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Effect of yeast polysaccharide on some hematologic parameter and gut morphology in channel catfish (Ictalurus punctatus)
作者：
Zhu, Huiling* ;Liu, Huiyu;Yan, Jing;Wang, Rui;Liu, Lihe
期刊：
Fish Physiology and Biochemistry ,2012年38(5):1441-1447 ISSN：0920-1742
通讯作者：
Zhu, Huiling
作者机构：
[Yan, Jing; Liu, Lihe; Zhu, Huiling; Liu, Huiyu; Wang, Rui] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Dept Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Zhu, Huiling] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Dept Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Yeast polysaccharides;Hematology;Intestinal morphology;Channel catfish
摘要：
A study was conducted to investigate the effect of dietary yeast polysaccharides on some hematologic parameters and intestinal morphology of channel catfish. Channel catfish were fed diets containing yeast polysaccharides at 0 (control), 0.1, 0.2, or 0.3 % for 7 weeks. Each diet was provided to 10 channel catfish specimens (5.82 ± 0.13 g initial weight) replicated 3 times in individual 250 L fiberglass tanks. Some hematologic parameters, leukocyte phagocytic activity, and intestinal morphology were monitored. After 7 weeks of trial, 0.2 % yeast polysaccharides resulted in significantly higher (P < 0.05) monocyte numbers. Furthermore, fish fed 0.2 % yeast polysaccharide diet had higher (P < 0.05) phagocytic rate of leukocyte. And 0.3 % yeast polysaccharide enhanced (P < 0.05) phagocytic index of leukocyte. Histological evaluation showed yeast polysaccharide supplementation increased the height of intestine fold (0.1, 0.2 and 0.3 %) and the thick of muscular layers (0.2 %) in intestine (P < 0.05). In addition, 0.1 and 0.3 % yeast polysaccharide supplementation improved the number of goblet cells (P < 0.05). The results of this trial indicate that yeast polysaccharides supplementation could affect blood monocytes, improve leukocytes phagocytic activity, and the development of intestine in channel catfish. © 2012 Springer Science+Business Media B.V.
语种：
英文
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稀土壳聚糖对鲫鱼非特异性免疫力的影响
作者：
汪明;刘军;黄峰;陈爱敬;刘立鹤;...
期刊：
粮食与饲料工业 ,2010年(9):52-54 ISSN：1003-6202
作者机构：
动物营养与饲料科学湖北省重点实验室,武汉工业学院,湖北,武汉,430023;[陈爱敬; 刘军; 汪明; 王锐; 黄峰; 刘立鹤] 武汉工业学院
关键词：
稀土壳聚糖螯合盐;鲫鱼;溶菌酶;超氧化物歧化酶;免疫器官指数
摘要：
研究稀土壳聚糖螯合盐对鲫鱼非特异性免疫力的影响。结果表明:饲喂20天后,3个试验组血清溶菌酶活性较对照组均有不同程度提高,且0.24%组血清溶菌酶显著高于对照组;但在第40、60天时,3个试验组血清溶菌酶活性与对照组相比差异均不显著。在第40天时,3个试验组血清超氧化物歧化酶活性均高于对照组;在第20、60天时,各组血清超氧化物歧化酶活性无明显变化规律,差异均不显著。第20天时,0.24%组鲫鱼脾脏指数、头肾指数均显著高于对照组,0.08%组、0.16%组脾脏指数、头肾指数也均高于对照组;但在第40、60天时,各组脾脏指数、头肾指数无明显变化规律,差异均不显著。
语种：
中文
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酵母多糖对斑点叉尾鮰免疫功能的影响
作者：
严晶;刘辉宇;朱惠玲;王锐;刘立鹤
期刊：
粮食与饲料工业 ,2009年(11):42-44 ISSN：1003-6202
作者机构：
武汉工业学院动物科学与营养工程学院,湖北,武汉,430023;[刘辉宇; 严晶; 王锐; 朱惠玲; 刘立鹤] 武汉工业学院
关键词：
酵母多糖;斑点叉尾鮰;免疫功能;吞噬活性
摘要：
研究在日粮中添加不同水平的酵母多糖对斑点叉尾鮰免疫功能的影响.结果表明:O.2%、0.3%酵母多糖组斑点叉尾细的头肾指数比对照组分别提高15.09%和7.50%,但试验组间差异不显著;0.3%酵母多糖组脾脏指数比对照组提高9.84%,但试验组间差异不显著.酵母多糖组斑点叉尾鮰血液中白细胞总数比对照组呈不同程度的降低,但各组间差异不显著.0.2%酵母多糖组斑点叉尾鮰白细胞吞噬率显著高于对照组和0.1%酵母多糖组,而0.2%、0.3%酵母多糖组间差异不显著;0.3%酵母多糖组斑点叉尾鮰吞噬指数比对照组显著提高29.77%.
语种：
中文
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