Using time-shared scanning optical tweezers assisted two-photon fluorescence imaging to establish a versatile CRISPR/Cas12a-mediated biosensor
作者：
Liu, Da;Sun, Xiao-Ming;Zhu, Lian;Li, Cheng-Yu
期刊：
Biosensors and Bioelectronics ,2023年227:115158 ISSN：0956-5663
通讯作者：
Li, Cheng-Yu(li-chengyu@wust.edu.cn)
作者机构：
[Liu, Da; Li, Cheng-Yu] Wuhan Univ Sci & Technol, Med Coll, Sch Publ Hlth, Hubei Prov Key Lab Occupat Hazard Identificat & Co, Wuhan 430065, Peoples R China.;[Sun, Xiao-Ming] Hubei Univ Med, Biomed Res Inst, Sch Basic Med Sci, Shiyan 442000, Peoples R China.;[Zhu, Lian] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Cheng-Yu Li] H;Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, 430065, PR China
关键词：
Biosensor;CRISPR;Imaging;Optical tweezers;Two-photon fluorescence
摘要：
Based on the admirable precision to identify target nucleic acids and the particular trans-cleavage feature, CRISPR/Cas12a system is a useful means to further improve the sensing accuracy and the design flexibility of fluorescence biosensors. However, the current construction concepts still suffer from insufficient sensitivity, unsuitable for complicated real samples and limited detection species. In this work, much efforts are achieved to address these obstacles. At first, we adopt a microsphere sustained signal enrichment, under which a home-made time-shared scanning optical tweezers assisted fluorescence imaging is employed to guarantee a stable excitation and also realize multiflux measurement. Furthermore, by taking advantage of the low background merit of the near-infrared light excited two-photon fluorescence, a commendable anti-interference capability is endowed to operate in complex media. After utilizing a functional DNA (e.g. aptamer and DNAzyme) regulated mediation pathway to respond non-nucleic acid analytes (alpha fetal protein and Pb2+), the newly-established CRISPR/Cas12a-mediated fluorescence biosensor is found to display favorable assay performance. More importantly, our analytical methodology can act as a versatile and reliable toolbox in various applications such as disease diagnosis and environmental analysis, propelling the development of CRISPR system in biosensing field. © 2023 Elsevier B.V.
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英文
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Novel montmorillonite nanosheets-based hydrogel beads with high adsorption performance and structural strength for removal of dyes and heavy metals
作者：
Zhang, Tingting;Li, Mingliang;Chen, Licai;Bai, Haoyu;Wang, Wei;...
期刊：
Chemical Physics Letters ,2023年813:140322 ISSN：0009-2614
通讯作者：
Chen, Licai(chenlc1219@whut.edu.cn)
作者机构：
[Zhang, Tingting] Wuhan Polytech Univ, Sch Chem & Environm Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.;[Li, Mingliang; Chen, Licai; Zhao, Yunliang] Wuhan Univ Technol, Sch Resources & Environm Engn, 34 Wenzhi St, Wuhan 430070, Hubei, Peoples R China.;[Bai, Haoyu] Imperial Coll London, Dept Mat, London SW7, England.;[Wang, Wei] Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Henan, Peoples R China.;[Zhao, Yunliang] Wuhan Clayene Technol Co Ltd, 36 Tangxunhu North Rd, Wuhan 430223, Hubei, Peoples R China.
通讯机构：
[Licai Chen; Yunliang Zhao] S;School of Resources and Environmental Engineering, Wuhan University of Technology, 34 Wenzhi Street, Wuhan, Hubei 430070, China<&wdkj&>School of Resources and Environmental Engineering, Wuhan University of Technology, 34 Wenzhi Street, Wuhan, Hubei 430070, China<&wdkj&>Wuhan Clayene Technology Co., Ltd., 36 Tangxunhu North Road, Wuhan, Hubei 430223, China
关键词：
Absorbents;Heavy metals;Hydrogel beads;Methylene blue;Montmorillonite nanosheets
摘要：
A series of high-performance montmorillonite nanosheets (MMTNS)-based hydrogel beads (HG-Bs) are prepared with different amounts of MMTNS, acrylic acid, acrylamide, sodium alginate and polyvinyl alcohol. MMTNS can significantly enhance the adsorption performance and structural strength, showing porous structure, good reusability, and high adsorption capacity of 564.97 mg/g for methylene blue. Moreover, FTIR, EDS and XPS measurements indicate that methylene blue is adsorbed by HG-Bs mainly through the adsorption between methylene blue and carboxyl, amide functional groups in HG-Bs, and the cation exchange. HG-Bs also has good adsorption capacity of 130.3 mg/g for Cu2+ and 155.7 mg/g for Cd2+. © 2023 Elsevier B.V.
语种：
英文
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Thermodynamics of Ligand Exchange with Aromatic Ligands on the Surface of CdSe Quantum Dots
作者：
Liu, Meng;Chen, Zhe-Yong;He, Xiao-Hang;Liu, Xing-Yu;Tian, Huan;...
期刊：
CHEMISTRY OF MATERIALS ,2023年35(5):1868-1876 ISSN：0897-4756
通讯作者：
Jiang, Feng-Lei(fljiang@whu.edu.cn)
作者机构：
[Liu, Xing-Yu; Tian, Huan; Liu, Yi; Jiang, Feng-Lei; Chen, Zhe-Yong; He, Xiao-Hang; Liu, Meng] Wuhan Univ, Coll Chem & Mol Sci, Sauvage Ctr Mol Sci, Wuhan 430072, Peoples R China.;[Liu, Yi] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Liu, Yi] Hubei Univ Sci & Technol, Hubei Key Lab Radiat Chem & Funct Mat, Xianning 437100, Peoples R China.
通讯机构：
[Feng-Lei Jiang] S;Sauvage Center for Molecular Sciences, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China
摘要：
Quantum dots (QDs) have great application prospects in optoelectronic devices because of their excellent optical properties. Nevertheless, the original ligands of the long carbon chain introduced in the synthesis process are not conducive to the application of CdSe QDs in optoelectronic devices due to their insulating nature. Therefore, short-chain and aromatic ligands are employed to replace the original ligands. However, the influences of conjugation structures on ligand exchange have rarely been systematically studied from a thermodynamic perspective. In this work, two types of aromatic ligands, namely, a series of benzoic acid (BA) ligands and cinnamic acid (CA) ligands that introduced electron-donating (methoxy-) and electron-withdrawing groups (fluoro-) at the meta and para positions of the benzene ring of aromatic ligands, were chosen to investigate the ligand exchanges on the surface of QDs from oleic acid (OA) to a series of aromatic ligands by 1H nuclear magnetic resonance (NMR) spectroscopy and isothermal titration calorimetry (ITC). 1H NMR showed that the average exchange ratio of CA ligands to OA was ∼1:1, which made it possible to obtain thermodynamic parameters through the determined ligand exchange model by ITC. Furthermore, thermodynamic results showed that ligand exchange with CA ligands was endothermic (ΔH > 0) and entropy-increasing (ΔS > 0), indicating that the process was entropy-driven. In addition, the equilibrium constants (K) of ligand exchange with CA ligands were larger than those with BA ligands. However, the K values of ligand exchange with BA ligands were ∼1, which resulted in ΔG ∼ 0 kJ/mol, demonstrating that ligand exchange with BA ligands was less favorable at 298.15 K. Finally, the introduction of substituents on meta and para positions of the aromatic ligand benzene ring had no significant effect on the ligand exchange process. This work not only elaborates on the process of exchange with aromatic ligands but also has fundamental significance for the application of aromatic ligand-modified QDs in optoelectronic devices. © 2023 American Chemical Society
语种：
英文
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Efficient peroxymonosulfate activation by magnetic CoFe2O4 nanoparticle immobilized on biochar toward sulfamethoxazole degradation: Performance, mechanism and pathway
作者：
Xiong, Minghui;Sun, Ya;Chai, Bo;Fan, Guozhi;Song, Guangsen
期刊：
Applied Surface Science ,2023年615:156398 ISSN：0169-4332
通讯作者：
Chai, Bo(willycb@163.com)
作者机构：
[Sun, Ya; Song, Guangsen; Chai, Bo; Xiong, Minghui; Fan, Guozhi] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Bo Chai] S;School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China
关键词：
CoFe2O4/BC composite;Degradation pathway;Peroxymonosulfate;Radical and non-radical
摘要：
Utilizing biomass waste to create highly efficient biochar-based catalysts has drawn intensively interest since it adheres to the idea of waste recycling in environmental conservation. Herein, CoFe2O4 nanoparticles immobilized on biochar (BC) derived from rape straw were synthesized and applied in degrading sulfamethoxazole (SMX) by activating peroxymonosulfate (PMS). Experimental results indicated that the 30 wt% CoFe2O4/BC composite catalyst had the best catalytic activity with the SMX degradation efficiency of 93% within 20 min, which was probably the fact that BC as a carrier not only effectively inhibited the agglomeration of CoFe2O4 nanoparticles and increased the active sites, but also could simultaneously participate in the catalytic degradation reaction as an activator of PMS. In addition, cycle experiments illustrated that the as-prepared catalyst possessed the excellent stability, and the magnetic property measurements implied that they were convenient for separation and recovery. According to quenching experiments, electron paramagnetic resonance (EPR) and electrochemical studies, both radical and non-radical process participated into degrading SMX, where the generated SO4·-, ·OH and O2·- as the major radical active species while 1O2 and direct electron transfer were responsible for the non-radical route. Besides, using high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis, a potential SMX degradation pathway was suggested. The present CoFe2O4/BC composite would be a promising PMS activation catalyst for environmental remediation. © 2023 Elsevier B.V.
语种：
英文
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Synthesis of 5‐hydroxymethylfurfural from glucose over carbon‐based acid–base bifunctional catalyst
作者：
Wang, Chun;He, Qiao;Chen, Wenhai;Cheng, Qunpeng;Song, Guangsen;...
期刊：
Energy Science & Engineering ,2023年11(7):2408-2420 ISSN：2050-0505
通讯作者：
Fan, Guozhi(fgzcch@163.com)
作者机构：
[Cheng, Qunpeng; Song, Guangsen; Chen, Wenhai; Wang, Chun; Fan, Guozhi; He, Qiao] Wuhan Polytech Univ, Coll Chem & Environm Engn, Wuhan, Peoples R China.;[Fan, Guozhi] Wuhan Polytech Univ, Coll Chem & Environm Engn, Wuhan 430023, Peoples R China.
关键词：
5-hydroxymethylfurfural;acid-base bifunctional catalyst;activated carbon;glucose
摘要：
In this paper, carbon‐based acid–base bifunctional catalyst was synthesized and employed for producing fuel precursor 5‐hydroxymethylfurfural from glucose, displaying excellent catalytic performance and stability. Abstract Carbon‐based acid–base bifunctional catalyst was synthesized by the functionalization of activated carbon (AC) with 3‐mercaptopropyl trimethoxysilane, 3‐aminopropyl trimethoxysilane, and H2SO4 via sulfhydrylation, ammonifiation, and sulfonation successively. The as‐prepared catalyst of NH2‐AC‐SO3H was characterized by infrared spectroscopy, X‐ray diffraction, thermogravimetric analysis and Brunauer–Emmet–Teller specific area analysis, and it was further used for producing fuel precursor 5‐hydroxymethylfurfural (5‐HMF) from glucose. The influences of the catalyst type, ratio of acid–base groups, catalyst amount, reaction temperature, and reaction time were investigated. It was found that NH2‐AC‐SO3H revealed excellent activity, and the yield of 5‐HMF reached 71.8% under the optimized reaction conditions with a 1.3:1 mass ratio of NH2‐AC‐SO3H to glucose at 140°C for 6 h in γ‐valerolactone. The reusability of NH2‐AC‐SO3H was also performed, and only slight decreases in the conversion of glucose and the yield of 5‐HMF were observed, still giving 65.3% yield of 5‐HMF after 4 runs.
语种：
英文
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Efficient degradation of COD from coking wastewater by corncob biochar-modified particles using a three-dimensional electrode reactor
作者：
Zhu, Qiaoyun;Liu, Xueling;Xiang, Jingjing;Li, Likun;Fu, Benquan;...
期刊：
Environmental Science: Water Research & Technology ,2023年9(6):1717-1728 ISSN：2053-1400
通讯作者：
Li, Likun(lilk@gwi.gd.cn);Zhang, Lei(zhanglei@whpu.edu.cn)
作者机构：
[Wang, Yi; Huang, Yanjun; Liu, Xueling; Zhu, Qiaoyun; Xiang, Jingjing; Fan, Guozhi; Zhang, Lei] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Li, Likun] Guangdong Acad Sci, China Ukraine Inst Welding, Guangzhou 510650, Peoples R China.;[Fu, Benquan] Wuhan Iron & Steel Co, R&D Ctr, Wuhan 430080, Peoples R China.
通讯机构：
[Likun Li] C;[Lei Zhang] S;China-Ukraine Institute of Welding, Guangdong Academy of Sciences, Guangzhou 510650, China<&wdkj&>School of Chemistry and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Fe-Mn/BC particle electrodes with different loading ratios were prepared by a sol-gel method using corncob biochar (BC) as a carrier. The particle electrodes were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD) and cyclic voltammetry (CV). Using the chemical oxygen demand (COD) removal efficiency of coking wastewater as an indicator of electrocatalytic oxidation performance, the influencing factors of the electrocatalytic oxidation performance by a three-dimensional electrode system are discussed and the analysis of the degradation mechanism was carried out. The results show that the Fe-Mn particle electrodes with a load ratio of 1 : 1 obtained after carbonization at 500 °C for 3 h had a COD removal rate of 81.26% under the optimum conditions of pH 7, a current density of 25 mA cm−2 and electrolysis time of 120 min. The three-dimensional electrode reactor (3DER) filled with Fe-Mn-modified BC particle electrodes showed superior performances. The three-dimensional fluorescence spectra results of the coking wastewater before and after treatment in the 3DER indicated that the refractory organics in the coking wastewater were degraded into degradable organic substances, and most of the soluble organic compounds were degraded and transformed. Moreover, the tert-butanol quenching experiment confirmed that the electrocatalytic process was mainly based on the indirect oxidation of hydroxyl radicals (˙OH) to achieve the removal of pollutants. This novel strategy realized the synergistic production of valuable corncob biochar particle electrodes and the remediation of pollutants with remarkable environmental and ecological benefits. © 2023 The Royal Society of Chemistry.
语种：
英文
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NIR-II-triggered photothermal therapy with Au@PDA/PEG-PI for targeted downregulation of PSMA in prostate cancer
作者：
Ding, Xin;Bai, Shiwei;Liu, Fachuang;Michal, Nowicki;Roman, Szewczyk;...
期刊：
Acta Biomaterialia ,2023年157:487-499 ISSN：1742-7061
通讯作者：
Peng, Na(pengna@wust.edu.cn)
作者机构：
[Liu, Fachuang; Liu, Yi; Peng, Na; Ding, Xin] Wuhan Univ Sci & Technol, Coll Chem & Chem Engn, Key Lab Coal Convers & New Carbon Mat Hubei Prov, Wuhan 430081, Peoples R China.;[Liu, Fachuang; Liu, Yi; Peng, Na; Ding, Xin] Wuhan Univ Sci & Technol, Inst Adv Mat & Nanotechnol, Coll Chem & Chem Engn, Wuhan 430081, Peoples R China.;[Liu, Yi] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Bai, Shiwei] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci BNLMS, CAS Key Lab Colloid Interface & Chem Thermodynam, Beijing 100190, Peoples R China.;[Liu, Yi] Hubei Univ Sci & Technol, Hubei Key Lab Radiat Chem & Funct Mat, Xianning 437100, Peoples R China.
通讯机构：
[Na Peng; Yi Liu] K;Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province & Institute of Advanced Materials and Nanotechnology, College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, PR China<&wdkj&>Belt and Road Joint Laboratory on Measurement and Control Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China<&wdkj&>Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province & Institute of Advanced Materials and Nanotechnology, College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, PR China<&wdkj&>School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China<&wdkj&>Hubei Key Laboratory of Radiation Chemistry and Functional Materials, Hubei University of Science and Technology, Xianning 437100, PR China
关键词：
NIR-II laser;Photothermal therapy;Prostate cancer;Prostate-specific membrane antigen
摘要：
Although positron emission tomography (PET) imaging products targeting prostate-specific membrane antigen (PSMA) have been approved for marketing, clinical challenges remain in the study of its use as a therapeutic target, such as the complex synthesis process and side effects after treatment. Here, we developed a strategy for targeted photothermal therapy (PTT) using PSMA as the target. The results of molecular docking demonstrated that the synthesized PEG modified urea-based PSMA inhibitor (small molecular PSMA inhibitor, PI) PI-PEG has a high affinity energy (binding energy = − 8.3 kcal mol−1) for the PSMA target. Therefore, modification of PI-PEG onto the surface of gold@polydopamine (Au@PDA) with NIR-II absorption could enable targeted PTT against PSMA. This work revealed that the prepared Au@PDA/PEG-PI were not only highly selective for PSMA, but also could efficiently ablate PSMA expression by targeted PTT at the maximum permissible exposure (MPE) of the NIR-II laser. Moreover, Au@PDA/PEG-PI also have potential for photoacoustic (PA) imaging and computed tomography (CT) imaging. As the first strategy to downregulate the expression of PSMA and successfully inhibit prostate cancer by targeted PTT, this study case provides a new idea for the clinical translation of PSMA as an integrated target for tumor diagnosis and anti-tumor treatment. Statement of significance: (1) Au@PDA/PEG-PI NPs were the novel PTT agent to target PSMA and successfully down-regulate PSMA expression. (2) Molecular docking results demonstrated that PI-PEG inhibitors have a high affinity energy for PSMA (binding energy = − 8.3 kcal mol−1). (3) Au@PDA/PEG-PI NPs can be targeted for efficient PTT at the MPE of the NIR-II laser. (4) Au@PDA/PEG-PI NPs also have the potential for PA and CT imaging. © 2022 Acta Materialia Inc.
语种：
英文
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Nascent disentangled UHMWPE: Origin, synthesis, processing, performances and applications
作者：
Wu, Sheng-Li;Qiao, Junfei;Guan, Jintao;Chen, Hong-Mei;Wang, Tielin;...
期刊：
European Polymer Journal ,2023年184:111799 ISSN：0014-3057
通讯作者：
Wang, Yi(ywang@wit.edu.cn)
作者机构：
[Wang, Yi; Wang, Cunwen; Wang, Tielin] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Wang, Yi; Wang, Cunwen; Wang, Tielin] Wuhan Inst Technol, Sch Chem & Engn Pharm, Key Lab Novel Reactor & Green Chem Technol Hubei P, Key Lab Green Chem Proc,Minist Educ, Wuhan 430205, Peoples R China.
通讯机构：
[Yi Wang] K;Key Laboratory for Green Chemical Process of Ministry of Education, Key Laboratory of Novel Reactor and Green Chemical Technology of Hubei Province, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430205, China
关键词：
Applications;Catalytic synthesis;Nascent d-UHMWPE;Origin;Processing
摘要：
The advent of nascent d-UHMWPE overcomes the intrinsic melting defects and crystal boundaries within the commercial UHMWPE (C-UHMWPE) based products, and opens the access to high value-added UHMWPE materials with long lifetime and improved qualities. The nascent d-UHMWPE derives from the disentanglement of UHMWPE that calls for suitable synthetic routes to d-UHMWPE. With a continuous development the synthesis of d-UHMWPE can be achieved either by single site catalysts or heterogeneous solid catalysts, and demonstrates a much easier processability within a wider temperature window. The processed d-UHMWPE demonstrates superior drawability, flexibility, toughness and thermal conductivity et al., and promises to be used in packages, bullet-proof armors, total knee/hip/ankle joint replacements, aerospace and marine engineering et al. The contribution systematically reviews the origin, catalytic synthesis, processing and applications of the nascent d-UHMWPE, and gives a detailed description on the conceptual proposal, the core and key points facing to the nascent d-UHMWPE. By analyzing the scientific problems still existing in the relating researches, the developmental trends and prospects of the nascent d-UHMWPE are envisioned. © 2022 Elsevier Ltd
语种：
英文
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Mesenchymal Stem Cell Derived Extracellular Vesicles: Promising Nanomedicine for Cutaneous Wound Treatment
作者：
Liu, Jia-Lin;Kang, De-Lai;Mi, Peng;Xu, Cheng-Zhi;Zhu, Lian;...
期刊：
ACS BIOMATERIALS SCIENCE & ENGINEERING ,2023年9(2):531-541 ISSN：2373-9878
通讯作者：
Zhu, Lian(yljzl@whpu.edu.cn);Wei, Ben-Mei(amei_xiaohua@163.com)
作者机构：
[Liu, Jia-Lin; Xu, Cheng-Zhi; Kang, De-Lai; Mi, Peng; Wei, Ben-Mei; Zhu, Lian] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Lian Zhu; Ben-Mei Wei] S;School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Changqing Garden, Wuhan, 430023 Hubei, China
关键词：
Skin wound;mesenchymal stem cells;extracellular vesicles;wound repair
摘要：
A skin wound represents a rupture caused by external damage or the existence of underlying pathological conditions. Sometimes, skin wound healing processes may place a heavy burden on patients, families, and society. Wound healing processes mainly consist of several continuous, dynamic, but overlapping stages, namely, the coagulation stage, inflammation stage, proliferation stage, and remodeling stage. Bacterial infection, excessive inflammation, impaired angiogenesis, and scar formation constitute the four significant factors impeding the recovery efficacy of skin wounds. This encourages scientists to develop multifunctional nanomedicines to meet challenging needs. As we know, mesenchymal stem cells (MSCs) have been widely explored for wound repair owing to their unique capability for self-renewal and multipotency. However, problems including immune concerns and legal restrictions should be properly resolved before MSC-based therapeutics are safely and widely used in clinics. Besides, maintaining the high viability/proliferation capability of MSCs during administration processes and therapy procedures is also one of the biggest technical bottlenecks. Extracellular vesicles (EVs) are cell-derived nanovesicles, that not only possess the basic characteristics and functions of their corresponding maternal cells but also contain several outstanding advantages including abundant sources, excellent biocompatibility, and convenient administration routes. Furthermore, the membrane surface and cavity are easy to flexibly modify to meet versatile application needs. Recently, MSC-derived EVs have emerged as promising therapeutics for skin wound repair. However, current reviews are too broad and rarely focused on the specific roles of EVs in the different stages of wound recovery. Therefore, it is quite necessary to demonstrate the significance of stem cell-derived EVs in promoting wound healing from several specific aspects. Here, this review primarily tries to provide critical comments on current advances in EVs derived from MSCs for wound repair, particularly elaborating on their impressive roles in effectively eliminating infections, inhibiting inflammation, promoting angiogenesis, and reducing scar formation. Last but not least, current limitations and future prospects of EVs derived from MSCs in the areas of wound repair are also objectively analyzed. © 2023 American Chemical Society.
语种：
英文
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Role of polyamide microplastic in altering microbial consortium and carbon and nitrogen cycles in a simulated agricultural soil microcosm
作者：
Sun, Xia;Tao, Ruidong;Xu, Daoqing;Qu, Mengjie;Zheng, Mingming;...
期刊：
Chemosphere ,2023年312(Pt 1):137155 ISSN：0045-6535
通讯作者：
Mei, Yunjun(meiyunjun_2000@163.com)
作者机构：
[Qu, Mengjie; Mei, Yunjun; Sun, Xia; Tao, Ruidong; Zhang, Meng; Zheng, Mingming] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Xu, Daoqing] Anhui Acad Agr Sci, Cotton Res Inst, Hefei 230031, Peoples R China.
通讯机构：
[Yunjun Mei] S;School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
关键词：
C cycle;Microbial community;Microplastic;N cycle;Polyamide microplastic
摘要：
Microplastics (MPs) are persistent organic pollutants globally, with a continuous increase in MP wastes near and away from the regions of human activities. Studies to date aimed to explore the impact of MPs on ecosystems, but the area of research could not go beyond environmental pollution caused by MPs. To address the menace of MPs, scientists need to pay enough attention to the biogeochemical cycles, microbial communities, and functional microorganisms. Hence, this study aimed to evaluate the impact of adding 0.3% (mass ratio) [low-concentration (LC) group] and 1% [high-concentration (HC) group] of polyamide (PA) MP to the soil microenvironment with regard to the aforementioned parameters. PA MP decreased the soil microbial diversity (Shannon and Simpson indices, P < 0.05). At the phylum level, PA MP increased the abundance of Acidobacteria, Firmicutes, and Crenarchaeota (P < 0.05); at the genus level, it enhanced that of Geobacter, Thiobacillus, Pseudomonas, and Bradyrhizobium (P < 0.01) while decreased that of Bacillus, Flavisolibacter, Geothrix, and Pseudarthrobacter (P < 0.05). PA MP affected the carbon (C) cycle. PA MP accelerated the soil C fixation by enhancing the abundance of the genes accA and pccA. The LC PA MP accelerated organic C degradation and methane metabolism by changing the abundance of mnp, chiA, mcrA, pmoA, and mmoX genes, while the HC PA MP inhibited them with increasing the experimental time. Regarding the effects of PA on the nitrogen (N) cycle, the PA MP promoted N assimilation and ammonification by increasing the abundance of the genes gdh and ureC, the impact of PA MP on N fixation and denitrification depended on its concentration and treating time. This study showed that PA MP impacted the microbial consortium, it also affected the C and N cycles and its effect depended on its concentration and the treating time. © 2022 Elsevier Ltd
语种：
英文
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Superlubricity by polyimide-induced alignment
作者：
Gao, Xinlei;Chen, Hao;Yan, Hong;Huang, Chengrui;Wu, Li;...
期刊：
摩擦(英文) ,2023年11(9):1690-1707 ISSN：2223-7690
通讯作者：
Gao, Xinlei(gaoxl0131@163.com)
作者机构：
[Gao, Xinlei] Hubei Univ, Sch Mat Sci & Engn, Wuhan 430062, Peoples R China.;[Wang, Tingting; Huang, Chengrui; Chen, Hao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Yan, Hong] Beijing Univ Chem Technol, Coll Chem, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China.;[Wu, Li] Wuhan Inst Technol, Sch Chem & Environm Engn, Wuhan 430074, Peoples R China.
通讯机构：
[Xinlei Gao] S;School of Materials Science and Engineering, Hubei University, Wuhan, China
关键词：
superlubricity;polyimide;liquid crystal;alignment
摘要：
We have investigated the lubrication alignment behavior of point-plane contact and plane-plane contact between the GCr15 steel and polyimide (PI) friction pair using nematic liquid crystals (LCs) as the lubricant. In this system, rubbing orients the macromolecular PI molecules, and the oriented PI molecules induce alignment of the LC molecules in contact with or close to the oriented PI molecules. The LC molecules are aligned in the wear scar grooves of the PI film, and alignment extends to the GCr15-steel counterpart. Alignment of the LC molecules is correlated with the strong interaction force between the PI and LC molecules, the stable coordination structure of the LCs and GCr15 steel, and the weak interaction between the LC molecules. We performed simulations of the pretilt angle of PI and LCs and discussed the relationship between the pretilt angle and the friction properties. Owing to the small pretilt angle between PI and the LCs, the LC molecules orient almost parallel to the PI material, which is beneficial for superlubricity of this type of friction system. [Figure not available: see fulltext.]. © 2023, The author(s).
语种：
英文
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A High Yield and Cost-Effective Pathway for the Production of Iron Doped Porous Carbon Derived from Squid Pen as Supercapacitor Electrode Material
作者：
Li, Sheng;Yang, Jingqi;Wang, Haibo;Chen, Lingyun
期刊：
Waste and Biomass Valorization ,2023年14(9):2815-2824 ISSN：1877-2641
通讯作者：
Wang, Haibo(wanghaibo216@126.com);Chen, Lingyun(lingyun1@ualberta.ca)
作者机构：
[Li, Sheng; Wang, Haibo] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.;[Li, Sheng; Chen, Lingyun; Yang, Jingqi] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada.
通讯机构：
[Haibo Wang] S;[Lingyun Chen] D;School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, People’s Republic of China<&wdkj&>Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada
关键词：
High yield;Iron activated;Porous carbon;Squid pen;Electrode material
摘要：
Researchers have devoted a lot of time and efforts to demonstrate the energy storage mechanism and improve the performance of biomass-based porous carbon materials for the past decades. But little attention has been paid to the cleanliness and low energy consumption of the manufacturing process, which is very important for the widely application in the practical production of porous carbon materials. As a fishery industry by-product, squid pen value-added utilizations have attracted attentions in recent years. This study reports an iron doped porous carbon derived from squid pen for supercapacitor application. The fabrication process are designed in a scientific and reasonable pathway, in which the activator—FeCl3 transforms into iron oxide improving the electrochemical performance meanwhile avoiding the repeated washing-centrifugation process of residual Fe. Optimal parameters of the synthesis process are Fe doped dosage (Squid pen: FeCl3 = 10:1, w/w), carbonization temperature 700°C for 1h and activation temperature 700°C for 1h. The specific capacitance of Fe doped carbonized squid pen powder (CSPP) has increased more than 4 times to 131.7 F/g at a current density of 0.2 A/g comparing to the CSPP. Fe doped CSPP also shows a relatively high yield of 30.6%, which is significantly higher than that of biomass based porous carbon material in previous reports. This high yield and cost-effective method is beneficial for the high value transformation of squid pen as electrode material application. Graphical Abstract: [Figure not available: see fulltext.]. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
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英文
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Magnetic optimization and regulation mechanism of La2CoMnO6 double-perovskite ceramics by Ti-doping at Mn-site
作者：
Shi, Junwei;Shen, Qiang;Wang, Chuanbin;Hu, Mingzhe
期刊：
Ceramics International ,2023年49(12):20662-20671 ISSN：0272-8842
通讯作者：
Hu, MZ
作者机构：
[Shi, Junwei] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Peoples R China.;[Hu, MZ; Hu, Mingzhe] Guizhou Minzu Univ, Coll Phys & Mechatron Engn, Guiyang 550025, Peoples R China.;[Shen, Qiang; Wang, Chuanbin] Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Peoples R China.
通讯机构：
[Hu, MZ ] G;Guizhou Minzu Univ, Coll Phys & Mechatron Engn, Guiyang 550025, Peoples R China.
关键词：
La2CoMnO6;Double-perovskite;Ti-doping;Magnetic optimization;First-principle calculation
摘要：
This work focuses on the magnetic optimization of La2CoMnO6 (LCMO) double-perovskite ceramics by Ti-doping at Mn-site. LCMO ceramics with different Ti-doping contents (LCMTxO, x = 0.1–0.4) were prepared by plasma activated sintering, and the effect of Ti-doping on the structural and magnetic properties of the ceramics is studied systematically. All the ceramics exhibit dense structure and small grain size, with a monoclinic P21/n crystalline structure. With the increasing of Ti-doping content, the proportions of Co2+ and Mn4+ in the LCMTxO ceramics change accordingly, reaching the highest values at x = 0.1. Since the Co2+-O-Mn4+ ferromagnetic super-exchange is the dominant factor affecting the magnetic properties of the LCMTxO ceramics, it is reasonable that a high saturation magnetization (Ms = 4.58μB/f.u) is obtained for the LCMT0.1O ceramic. The effect of Ti-doping on the orbital structure and magnetic regulation of the LCMTxO double-perovskite ceramics is further investigated by the first-principle calculation. It is found that the magnetic moments of Co and Mn ions will increase by the appropriate addition of Ti, which makes most Co ions exist in the form of Co2+, underpinning the La2Co2+Mn4+O6 ferromagnetic state and improving the magnetic properties. © 2023
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英文
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Anaerobic digestion of sulphate wastewater mediated by biochar
作者：
Xu, Chenxi;Ding, Yongyu;Liu, Jiacheng;Huang, Wenwen;Cheng, Qunpeng* ;...
期刊：
Environmental Technology ,2023年44(11):1667-1678 ISSN：0959-3330
通讯作者：
Cheng, Qunpeng
作者机构：
[Ding, Yongyu; Cheng, Qunpeng; Liu, Jiacheng; Song, Guangsen; Zhang, Shunxi; Yan, Juntao; Huang, Wenwen; Fan, Guozhi; Xu, Chenxi] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430073, Peoples R China.;[Xiao, Bo] Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan, Peoples R China.
通讯机构：
[Cheng, Qunpeng] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430073, Peoples R China.
关键词：
Sulphate wastewater;biochar;anaerobic digestion;COD removal rate;methanogenesis
摘要：
In this paper, the influences of biochar on the anaerobic digestion of sulphate wastewater, including the COD removal rate, methane yield, intermediate products and the change of microbial community structure, were investigated. The results showed that sulphate could promote the anaerobic digestion with the SO42−/COD ratio increasing from 0 to 0.1, while the activity of MPB was inhibited, which led to the decrease of COD removal rate and methane yield with the SO42−/COD ratio increasing from 0.1 to 2. At 1 g biochar loading, 344.97 mL CH4/gCODremoval was obtained compared with the control group (220.70 CH4/gCODremoval) at 2 of SO42−/COD. Biochar could also reduce the secondary accumulation of NH4+–N and TVFA. Meanwhile, methanogenic microorganisms were selectively enriched especially for methanobacterium, methanosaeta and methanolinea, while the growth of SRB was inhibited with biochar addition. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
语种：
英文
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Superhydrophobic polyaniline solid contact for potential stability improvement of NH4+-selective electrode
作者：
Bao, Yanzhou;Yan, Jiabao;Hu, Jie;Li, Jing
期刊：
Sensors and Actuators B-Chemical ,2023年390:133997 ISSN：0925-4005
通讯作者：
Yan, JB
作者机构：
[Bao, Yanzhou; Li, Jing; Yan, Jiabao] Wuhan Univ Sci & Technol, Sch Chem & Chem Engn, Hubei Prov Key Lab Coal Convers & New Carbon Mate, 947 Peace Ave, Wuhan 430081, Peoples R China.;[Hu, Jie] Wuhan Polytechn Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Yan, JB ] W;Wuhan Univ Sci & Technol, Sch Chem & Chem Engn, Hubei Prov Key Lab Coal Convers & New Carbon Mate, 947 Peace Ave, Wuhan 430081, Peoples R China.
关键词：
NH4+ -selective electrode;Solid contact;Superhydrophobic polyaniline;Water layer;Potential stability
摘要：
Potential stability and reproducibility of solid-contact ion-selective electrodes (SC-ISEs) are key to ensure reliable accuracy of actual water sample monitoring. However, undesirable water layer formed at interface of SCs and ion-selective membranes inevitably changes the interfacial potential. Here a superhydrophobic polyaniline (PANI) SC is presented to improve potential response of NH4+-ISE. Electrodeposition methods are used to control PANI growth and perfluorooctanoic acid co-doping, forming PANI SCs with different wettabilities. Though constructed SC-NH4+-ISEs show detection linear ranges from 10-5 to 10-1 M, superhydrophobic SC-ISE has a slope (58.07 mV/dec) closest to the ideal Nernstian slope. Importantly, superhydrophobic SC greatly inhibits water layer formation and enables SC-NH4+-ISE to have a very stable potential response. Potential drift is only 13.6 ± 3.2 µV/h for 12 h continuous measurement and standard deviation of the standard potential is 0.96 mV (n = 3), which are 55.7 and 5.4 times lower than those of unmodified SC-ISE, respectively. Moreover, superhydrophobic SC-ISE exhibits excellent potential stability against external interferences and even during actual wastewater monitoring. Once water layer is formed in unmodified SC-ISE, inaccuracy of measured NH4+ concentrations in actual wastewater reaches ca. 37%. This study demonstrates that superhydrophobic SC can efficiently improve potential response of SC-ISEs. © 2023 Elsevier B.V.
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英文
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A fluorescent sensing platform based on collagen peptides-protected Au/Ag nanoclusters and WS2 for determining collagen triple helix integrity
作者：
Li, Ke;Kang, Delai;Li, Yu;Zhu, Weizhe;Zhu, Lian;...
期刊：
Analytica Chimica Acta ,2023年1247:340900 ISSN：0003-2670
通讯作者：
Wang, Haibo(wanghaibo@whpu.edu.cn);Wei, Benmei(benmeiwei@whpu.edu.cn)
作者机构：
[Kang, Delai; Li, Yu; Xu, Chengzhi; Zhu, Weizhe; Wei, Benmei; Li, Ke; Wang, Haibo; Zhu, Lian; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Benmei Wei; Haibo Wang] S;School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, 430023, PR China
关键词：
Au/Ag nanoclusters;Collagen;Triple helix integrity;WS(2)
摘要：
The unique triple helix structure of collagen plays an important role in its biological properties, and the triple helix integrity is closely correlated with its molecular behavior and biological functions. Nevertheless, there is still a lack of convenient, accurate and practical methods for quantitatively determining collagen triple helix integrity. Herein, we first prepared bovine skin collagen peptide (BSCP)-protected Au/Ag nanoclusters (Au/AgNCs@BSCP) with excellent optical properties, high stability and good biocompatibility, which could adsorb on WS2 surface leading to fluorescence quenching. Upon the addition of collagen, the interaction of collagen and Au/AgNCs@BSCP led to the detachment of Au/AgNCs@BSCP from the WS2 surface, causing an increase in the fluorescence signal. Using the difference in the fluorescence recovery of the different samples, we achieved the quantitative determination of collagen triple helix integrity. This developed strategy exhibited excellent accuracy, selectivity, and practicality, thus showing promising potentials in biomedical applications. © Elsevier B.V.
语种：
英文
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Co-production of 5-hydroxymethyl furfural and furfural from holocellulose over UiO-66-derived acid-base catalysts
作者：
Fan, Guozhi;Chen, Yi;Lu, Yuchan;Cheng, Qunpeng;Chai, Bo;...
期刊：
Chemical Engineering Science ,2023年276:118768 ISSN：0009-2509
通讯作者：
Fan, GZ
作者机构：
[Chen, Yi; Cheng, Qunpeng; Lu, Yuchan; Song, Guangsen; Chai, Bo; Fan, Guozhi] Wuhan Polytech Univ, Coll Chem & Environm Engn, Wuhan 433023, Peoples R China.;[Song, Guangsen] Agr Resources Chem & Chem Utilizat Engn Res Ctr, Wuhan 430023, Peoples R China.
通讯机构：
[Fan, GZ ] W;Wuhan Polytech Univ, Coll Chem & Environm Engn, Wuhan 433023, Peoples R China.
关键词：
5-Hydroxymethyl furfural;Furfural;Holocellulose;UiO-66-derived acid-base catalyst
摘要：
UiO-66-derived H2N-UiO-66-SO3H with acid-base active sites was used for the co-production of 5-hydroxymethyl furfural (5-HMF) and furfural (FUR) in the direct conversion of holocellulose composed of cellulose and hemicellulose. The prepared catalyst was further characterized by various techniques, confirming its formation, structure, and composition. H2N-UiO-66-SO3H displayed excellent performance for the selective conversion of holocellulose to 5-HMF and FUR, achieving yields of 39.1 % and 28.2 %, respectively. The reusability of the catalyst was also investigated by direct use after recycling without any pretreatment. The results indicated that H2N-UiO-66-SO3H had good stability, and after four cycles, the yields of 5-HMF and FUR decreased only slightly to 37.2 % and 27.1 %, respectively. The recyclable catalyst H2N-UiO-66-SO3H for the effective conversion of holocellulose to 5-HMF and FUR demonstrated application potential for the utilization of lignocellulosic biomass. © 2023 Elsevier Ltd
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英文
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The volcanic relationship between the HER activity and lattice constant of RuCo alloy nanofilms
作者：
Wang, Yanyan;Ren, Zhandong* ;Cong, Ning;Heng, Yaping;Wang, Ming;...
期刊：
Chemical Communications ,2022年58(29):4631-4634 ISSN：1359-7345
通讯作者：
Ren, Zhandong;Zhu, YC
作者机构：
[Wang, Ziqian; Ren, Zhandong; Liu, Ye; Wang, Yanyan; Zhu, Yuchan; Cong, Ning; Xie, Zhiqiang; Han, Juanjuan; Heng, Yaping; Wang, Ming; Zhu, YC] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Ren, ZD; Zhu, YC ] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
摘要：
RuCo-ANFs with different proportions can be successfully prepared by magnetron sputtering, and their lattice constants can be adjusted accurately. In the RuCo-ANF, there is an obvious electronic interaction between Ru and Co, thus adjusting the ΔGH* on its surface. There is a volcanic relationship between the HER activity and the lattice constant of RuCo-ANF. © 2022 The Royal Society of Chemistry.
语种：
英文
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Photocatalytic fuel cell – A review
作者：
He, Yun;Chen, Keda;Leung, Michael K. H.* ;Zhang, Yizhen;Li, Li;...
期刊：
Chemical Engineering Journal ,2022年428:131074 ISSN：1385-8947
通讯作者：
Leung, Michael K. H.
作者机构：
[Li, Jianfen; He, Yun] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.;[Leung, Michael K. H.; Zhang, Yizhen; He, Yun; Chen, Keda] City Univ Hong Kong, Abil R&D Energy Res Ctr, Sch Energy & Environm, Hong Kong, Peoples R China.;[Zhang, Yizhen] Dalian Univ Technol, Sch Environm Sci & Technol, Key Lab Ind Ecol & Environm Engn Minist Educ, Dalian 116024, Peoples R China.;[Li, Li] Jiangsu Univ Technol, Sch Automot & Traff Engn, Changzhou 213001, Jiangsu, Peoples R China.;[Li, Guisheng] Univ Shanghai Sci & Technol, Sch Mat Sci & Engn, Shanghai 200093, Peoples R China.
通讯机构：
[Leung, Michael K. H.] C;City Univ Hong Kong, Abil R&D Energy Res Ctr, Sch Energy & Environm, Hong Kong, Peoples R China.
关键词：
Cell configurations;Photocatalytic fuel cell;Photoelectrodes;Recovery of chemical energy;Synergistic mechanisms
摘要：
Photocatalytic oxidation has been widely investigated and applied to perform degradation of organic pollutants in water and air. In recent technological advancement, photocatalysis (PC) is integrated into fuel cell (FC) to form photocatalytic fuel cell (PFC) for simultaneous wastewater treatment and production of electricity. In the PFC mechanisms, the organic pollutant, acting as a fuel in the fuel cell component, is decomposed upon light activation at the photoanode and the flow of photoexcited electrons is driven by the potential difference between the two electrodes. Thus, unwanted electron-hole recombination is effectively inhibited, resulting in enhanced PC activity. In other words, the chemical energy stored in the organic pollutant is recovered and converted into useful electricity during the wastewater treatment process. The photoelectrochemical technology can also be implemented for hydrogen generation and carbon dioxide reduction. Various strategies have been investigated for improving the PFC mechanisms through better visible-light photoelectrodes, innovative cell designs, dual-photoelectrode setup, as well as optimal control. In this review, the fundamentals and technological development of PFC will be discussed with special attention to novel cell configurations. With better knowledge and understanding of the PFC, we can identify promising research directions to further develop the PFC technologies. © 2021 Elsevier B.V.
语种：
英文
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Preparation of Ni-La/Al2O3-CeO2-Bamboo Charcoal Catalyst and Its Application in Co-pyrolysis of Straw and Plastic for Hydrogen Production
作者：
Zhang, Yiran;Li, Jianfen* ;Li, Bolin;Li, Zeshan;He, Yun;...
期刊：
BioEnergy Research ,2022年15(3):1501-1514 ISSN：1939-1234
通讯作者：
Li, Jianfen
作者机构：
[Li, Jianfen; Qin, Zhenhua; He, Yun; Zhang, Yiran; Gao, Rongyi; Li, Bolin; Li, Zeshan] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Li, Jianfen] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
关键词：
Composite support;Co-pyrolysis;Catalyst;Hydrogen yield;Catalytic performance
摘要：
The conversion of biomass and plastic into hydrogen through catalytic pyrolysis is a promising technology for the production of clean fuels. The developed Ni-La/Al2O3-CeO2-Bamboo charcoal (ACB) catalyst was applied to the co-pyrolysis of straw and polyethylene (PE) to produce hydrogen. This research explored the optimal mixing ratio of straw and PE, and the influence of the presence and stability of the catalysts on hydrogen production. Experiments show that the best mixing ratio is 5:5. Ni-La/ACB can significantly promote the production of H2 (13.56mmol/g), and it can still maintain high stability after repeated use, which is inseparable from the introduction of La to improve the performance of the catalyst. It can be concluded that adding a certain proportion of plastic is beneficial to increase H2 production, and co-pyrolysis can solve the pollution problem caused by improper treatment of plastics while making full use of renewable energy. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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