作者机构:
[Wang, Hongxun; Zou, Han; Meng, Shutong; Wang, Huajuan; Zhou, Min; Zhou, M; Ma, Jiale] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Wang, Hongxun; Wang, Huajuan; Zhou, Min; Zhou, M] Hubei Ind Technol Res Inst Jingchu Special Foods, Jingzhou 434000, Peoples R China.
通讯机构:
[Zhou, M ] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;Hubei Ind Technol Res Inst Jingchu Special Foods, Jingzhou 434000, Peoples R China.
摘要:
The rapid emergence and spread of drug-resistant pathogens pose a serious threat to human health. To overcome this problem, the exploration of new antibacterial agents, especially antibiotic-free strategies, is urgent. Here, nitric oxide (NO) release and photothermal effects were integrated into a single nanoplatform to achieve more efficient antibacterial effects. The nanoplatform (SNP/MPDA) was composed of mesoporous polydopamine (MPDA) and sodium nitroferricyanide (III) dihydrate (SNP). Near-infrared (NIR)-light could make MPDA produce high temperature, which could not only effectively kill bacteria but also induce SNPs to generate NO to kill bacteria. The NO/photothermal strategy showed the ability to kill both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureusand destruct mature biofilm. This antibiotic-free strategy had the advantage of producing NO in situ, thereby showing its high antibacterial activity and great potential in killing pathogens. This work provides insights into the design of more efficient antimicrobial strategies without antibiotics.
摘要:
Electronic cigarettes (e-cigarette) have become more and more popular among young smokers in recent years. Nicotine, the main addictive component in cigarettes, is widely present in many e-cigarette. Assessing levels of nicotine is critical to the health of consumers and the development of tobacco industry. Herein, a novel electrochemical sensor based on Ag/TiO 2 nanoparticles, metal - organic frameworks (MOFs) and graphene oxide (dual-NPs@HKUST-1/GO) nanocomposite was prepared for the rapid detection of nicotine in e-cigarette. The composites were characterized by scanning electron microscopy, X-ray diffraction, Raman spectroscopy and Fourier transform infrared spectroscopy, respectively. The results showed that the successfully synthesized composites have an average size of (20 +/- 5) mu m and excellent characterization properties. Under optimized conditions, the peak current response of nicotine with the differential pulse voltammetry showed a linear relationship in the range of 0.025 - 300 mu M with a detection limit of 0.0145 mu M. This newly developed dual-NPs@HKUST-1/GO-based sensor exhibited excellent selectivity, sensitivity and reproducibility for the accurate detection of nicotine. In addition, it also has excellent performance in detecting nicotine in actual e-cigarette refill liquids. The recoveries ranged from 88.6 % to 101.8 % in e-cigarette refill liquids, indicating good detection performance. This method provides a new concept for the detection of nicotine in the tobacco industry and clinical fields.
摘要:
Vibrio parahaemolyticus is a common foodborne pathogenic bacterium. With the overuse of antibiotics, an increasing proportion of drug-resistant strains are emerging, which puts enormous pressure on public health. In this study, a V. parahaemolyticus-specific phage, VP41s3, was isolated. The head length, width, and tail length of the phage were 77.7 nm, 72.2 nm, and 17.5 nm, respectively. It remained active in the temperature range of 30-50°C and pH range of 4-11. The lytic curve of phage VP41s3 showed that the host bacteria did not grow until 11 h under phage treatment at MOI of 1000, indicating that the phage had good bacteriostatic ability. When it was added to shellfish contaminated with V. parahaemolyticus (15°C, 48 h), the number of bacteria in the experimental group was 2.11 log(10) CFU/mL lower than that in the control group at 24 h. Furthermore, genomic characterization and phylogenetic analysis indicated that phage VP41s3 was a new member of the Podoviridae family. The genome contained 50 open reading frames (ORFs), in which the ORF19 (thymidine kinase) was an enzyme involved in the pyrimidine salvage pathway, which might lead to the accelerated DNA synthesis efficiency after phage entered into host cells. This study not only contributed to the improvement of phage database and the development of beneficial phage resources but also revealed the potential application of phage VP41s3 in food hygiene and safety.
摘要:
Photothermal therapy (PTT) is considered to be one of the promising methods to combat pathogenic bacteria. However, traditional PTT is prone to generate undesired temperature increase to surrounding normal tissues, which limits the application of PTT. Herein, an acid-responsive PTT system (Au nanoparticles system: AuNPs-S) was constructed based on the photothermal feature of spherical gold nanoparticles (AuNPs) and the low pH of the bacterial infected site. AuNPs-S is composed of two kinds of AuNPs: AuNPs modified with Asp-Asp-Asp-Asp-Asp-Cys (peptide A) were denoted as AuNPs-A; AuNPs modified with 2,3-dimethylmaleic anhydride (DA) grafted Lys-Gly-Gly-Lys-Gly-Gly-Lys-Cys (peptide B) were denoted as AuNPs-B/DA. AuNPs-B/DA with an acid-responsive moiety showed a charge-convertible feature. The negatively charged AuNPs-B/DA became positively charged AuNPs-B at low pH, aggregating with the negatively charged AuNPs-A via an electrostatic interaction, reaching the threshold to the interparticle plasmonic coupling effect among AuNPs, thereby killing bacteria precisely under the irradiation of near-infrared (NIR) light through the elevated temperature at the targeted area. This acid-responsive PTT strategy supplies an excellent mode for combating bacterial infections with no vital damage to normal tissues.
期刊:
FOODBORNE PATHOGENS AND DISEASE,2023年20(4):149-157 ISSN:1535-3141
通讯作者:
Zhou, M.;Wang, H.
作者机构:
[Wang, Huajuan; Zhou, Min; Hou, Wenfu; Liu, Wenting; Hao, Lulu] Wuhan Polytech Univ, Sch Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430024, Peoples R China.;[Wang, Hongxun; Xia, Hai] Wuhan Polytech Univ, Sch Life Sci & Technol, 68 Xuefu South Rd, Wuhan 430024, Peoples R China.
通讯机构:
[Hongxun Wang; Min Zhou] S;School of Food Science and Engineering and Wuhan Polytechnic University, Wuhan, China.<&wdkj&>School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China.
摘要:
In order to explore the application prospects of phages for controlling bacterial contamination, a lytic phage Pf17397_F_PD1 (Later abbreviated as PD1) was isolated from fish guts using Pseudomonas fluorescens ATCC 17397 as the host bacterium. The phage displayed short latency (18 min), long lysis period (212 min), and high lysis volume (1.47 x 102 PFU/each cell). It displayed wide temperature (30-70 & DEG;C) and pH (4-11) tolerance. Genomic comparison revealed a maximum sequence identity of 48.65% between phage PD1 and other identified phages, indicating that PD1 was a new phage. The phage PD1 significantly inhibited the growth of P. fluorescens in milk and grass carp at 4 & DEG;C and 25 & DEG;C. Compared to the negative control, bacterial levels in milk stored at 25 & DEG;C for 48 h were reduced by 2.71 log CFU/mL and 2.84 log CFU/mL at the multiplicity of infection (MOI) of 100 and 1,000, respectively. In contrast, when grass carp were stored at 25 & DEG;C for 24 h, the bacterial load was reduced by 1.28 log CFU/g and 2.64 log CFU/g compared to the control (MOI of 100 and 1,000). When the phage was applied for preservation of grass carp blocks, total volatile salt nitrogen (TVB-N) values of phage-treated samples increased by 6.8 mg/100 g and 7.5 mg/100 g at MOI of 100 and 1,000, respectively, after 7 days of storage, which was significantly lower than that of the control group (15.83 mg/100 g). This study showed that phage PD1 was a good natural biological antimicrobial agent against P. fluorescens ATCC 17397.
作者机构:
[Wang, Hongxun; Zou, Han; Wang, Huajuan; Zhou, Min; Wu, Yiming; Wang, Yudong] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Wang, Hongxun; Wang, Huajuan; Zhou, Min] Hubei Ind Technol Res Inst Jingchu Special Foods, Jingzhou 434000, Peoples R China.;[Song, Zhiyong] Huazhong Agr Univ, Coll Sci, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China.
通讯机构:
[Hongxun Wang; Min Zhou] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, P. R. China<&wdkj&>Hubei Industrial Technology Research Institute of Jingchu Special Foods, Jingzhou, 434000, P. R. China
摘要:
The synthesis of functional antibacterial nanoplatforms for killing pathogenic bacteria is urgent. Among them, combining traditional antibiotics with antimicrobial photodynamic therapy (PDT) is an attractive means. Here, we synthesized a multimodal antibacterial nanoplatform (denoted as Van/PCN) based on PCN-224 and vancomycin (Van). PCN-224 is a kind of photosensitizer-based nanoscale metal organic framework (pMOF), which not only possessed large surface area and many binding sites for loading Van but also had the ability to produce ROS under visible light irradiation. Van/PCN killed bacteria by Van and ROS, achieving a synergistic antibacterial effect. Since Gram-positive bacteria are sensitive to Van, this antibacterial nanoplatform has great potential to treat bacterial infections caused by Gram-positive pathogens.
作者:
Hai Xia;Na Yan;Jiaqi Jin;Wenfu Hou;Hongxun Wang;...
期刊:
FOODBORNE PATHOGENS AND DISEASE,2022年19(8):543-549 ISSN:1535-3141
通讯作者:
Zhou, M.;Wang, H.
作者机构:
[Hai Xia; Na Yan; Jiaqi Jin; Wenfu Hou] Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.;Address correspondence to: Min Zhou, PhD, Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Changqing Garden, Wuhan 430023, China;Address correspondence to: Hongxun Wang, PhD, Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Changqing Garden, Wuhan 430023, China;[Hongxun Wang] Address correspondence to: Hongxun Wang, PhD, Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Changqing Garden, Wuhan 430023, China<&wdkj&>Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.;[Min Zhou] Address correspondence to: Min Zhou, PhD, Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Changqing Garden, Wuhan 430023, China<&wdkj&>Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.
通讯机构:
[Hongxun Wang; Min Zhou] A;Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.
摘要:
This study used response surface methodology to determine the optimal conditions for extraction of polysaccharides from Pyracantha. fortuneana (PSPF), and studied the mechanism of PSPF-inducing apoptosis in human ovarian carcinoma Skov3 cells. Response surface methodology (RSM) were adopted to extract PSPF. The maximum value of polysaccharide yield was obtained under these optimal conditions. PSPF had good potential as an antioxidant. Exposure of cells to PSPF resulted in cytotoxicity through the induction of apoptosis, and the reactive oxygen species were increased, mitochondrial membrane potential decreased, DNA damage (detected as gamma- H2AX and RAD51 foci) was observed in Skov3 cells. In addition, PSPF could induce apoptosis of cancer cells. Therefore, PSPF should be explored as novel potential antioxidants and an anti-tumor drug in a clinical setting.
摘要:
The oxidation of phenolic compounds caused by polyphenol oxidase (PPO) often contributes to the browning and decaying of lotus root. However, methods are limited to solve this problem and to effectively extend the shelf life of fresh lotus. Thus, lactic acid bacteria (LAB) were used in this study as an alternative solution. Michaelies-Menten equations analysis indicated that gallic acid, chlorogenic acid and catechin of lotus were the most optimal substrates of PPO. So Lactobacillus plantarum (LH-B02), Lactobacillus acidophilus (LA-05) and Lactobacillus casei (LC-01) were cultivated at the presence of gradient concentrations of these phenols, presenting different dose-effect relationship. The decomposition of polyphenols by effective strains was detected by HPLC analysis, indicating that after interacted for 30 h, 84.17% of catechin was transformed by LH-B02. When LH-B02 suspension (1.44 × 1011 CFU L−1) were sprayed on the surface of the lotus root for 1, 3, 5, and 8 times, the color loss of treated lotus root was significantly reduced (p < 0.01), compared with the control during the storage of 15 d. Texture properties involving hardness, chewiness, springiness and cohesiveness of the lotus was significantly (p < 0.05) enhanced, especially for the samples sprayed five and eight times, suggesting that LAB could improve the postharvest properties of lotus root.
摘要:
Lotus (Nelumbo nucifera Gaertn) is a wetland vegetable famous for its nutritional and medicinal value. Phenolic compounds are secondary metabolites that play important roles in the browning of fresh-cut fruits and vegetables, and chemical constituents are extracted from lotus for medicine due to their high antioxidant activity. Studies have explored in depth the changes in phenolic compounds during browning, while little is known about their synthesis during the formation of lotus rhizome. In this study, transcriptomic analyses of six samples were performed during lotus rhizome formation using a high-throughput tag sequencing technique. About 23 million high-quality reads were generated, and 92.14% of the data was mapped to the reference genome. The samples were divided into two stages, and we identified 23,475 genes in total, 689 of which were involved in the biosynthesis of secondary metabolites. A complex genetic crosstalk-regulated network involved in the biosynthesis of phenolic compounds was found during the development of lotus rhizome, and 25 genes in the phenylpropanoid biosynthesis pathway, 18 genes in the pentose phosphate pathway, and 30 genes in the flavonoid biosynthesis pathway were highly expressed. The expression patterns of key enzymes assigned to the synthesis of phenolic compounds were analyzed. Moreover, several differentially expressed genes required for phenolic compound biosynthesis detected by comparative transcriptomic analysis were verified through qRT-PCR. This work lays a foundation for future studies on the molecular mechanisms of phenolic compound biosynthesis during rhizome formation.
作者:
Zhou, Min;Li, Xiaofang;Hou, Wenfu;Wang, Hongxun*;Paoli, George C.;...
期刊:
Frontiers in Microbiology,2019年10:ARTN 2265 ISSN:1664-302X
通讯作者:
Wang, Hongxun;Shi, Xianming
作者机构:
[Zhou, Min; Li, Xiaofang; Hou, Wenfu; Wang, Hongxun] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China;[Paoli, George C.] ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastem Reg Res Ctr,Most Joint Res Ctr Food Safety, Wyndmoor, PA USA;[Shi, Xianming] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, Shanghai, Peoples R China
通讯机构:
[Wang, Hongxun] W;[Shi, Xianming] S;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China. Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, Shanghai, Peoples R China.
关键词:
MLST;Salmonella;blaCTX-M-15;blaCTX-M-65;class I integrons;qnrB
摘要:
Salmonella is a leading cause of foodborne disease and is often associated with the consumption of foods of animal origin. In this study, sixty-six Salmonella isolates were obtained from 631 raw meat samples purchased at small retail suppliers in Hubei Province, China. The most prevalent Salmonella serotypes were Thompson (18.2%) and Agona (13.6%). Frequent antimicrobial resistance was observed for the sulfonamides (43.9%), tetracycline (43.9%), and the b-lactams amoxicillin and ampicillin (36.4% for each). Interestingly, a high incidence of resistance to cephazolin was observed in strains of the most common serotype, S. Thompson. Class I integrons were found in 27.3% (18/66) of the isolates and five of these integrons contained different gene cassettes (aacA4C-arr-3-dfr2, dfrA12-aadA21, aadA2, dfrA12-aadA2, dfr17-aadA5). Additional antimicrobial resistance genes, including blaTEM-1, blaCTX- M-65, blaCTX- M-15, qnrB, and qnrS, were also identified among these Salmonella isolates. Results of replicon typing and conjugation experiments revealed that an integron with qnrB and blaCTX- M-15 genes was present on incH12 mobile plasmid in S. Thompson strain. Multilocus sequence typing (MLST) analysis revealed 32 sequence types, indicating that these isolates were phenotypically and genetically diverse, among which ST26 (18.2%) and ST541 (12.1%) were the predominant sequence types. The integrons, along with multiple antimicrobial resistance genes on mobile plasmids, are likely contributors to the dissemination of multidrug resistance in Salmonella.
关键词:
acid-sensing ion channels;Asic2a;Asic4;methylation;morris water maze;walnut oil
摘要:
<jats:title>Abstract</jats:title><jats:p>Although Walnut oil (<jats:styled-content style="fixed-case">WO</jats:styled-content>) has been reported to enhance cognitive function, the underlying molecular mechanisms are not well understood. This study was designed to assess the effects of <jats:styled-content style="fixed-case">WO</jats:styled-content> on spatial memory in rats through modulation of the expression of acid‐sensing ion channel genes, <jats:italic>Asic2a</jats:italic> and <jats:italic>Asic4</jats:italic>. To investigate the effect of <jats:styled-content style="fixed-case">WO</jats:styled-content> on cognitive performance, we supplemented the diet of female rats with <jats:styled-content style="fixed-case">WO</jats:styled-content>. The results showed that supplementation with <jats:styled-content style="fixed-case">WO</jats:styled-content> at doses of 2.2 and 11gkg<jats:sup>−1</jats:sup>day<jats:sup>−1</jats:sup> significantly improved learning and memory. In vitro treatment of rat hippocampal neuronal cells with appropriate doses of <jats:styled-content style="fixed-case">WO</jats:styled-content> revealed a significant increase in the expression of <jats:italic>Asic2a</jats:italic> and <jats:italic>Asic</jats:italic>4 in a dose‐dependent manner at both the <jats:styled-content style="fixed-case">mRNA</jats:styled-content> and protein levels. We conclude that <jats:styled-content style="fixed-case">WO</jats:styled-content> intake might help to prevent cognitive decline, particularly in the elderly, and that <jats:styled-content style="fixed-case">ASIC</jats:styled-content> genes in neurons can be the targets of compounds contained in the oil.</jats:p>