作者： Li, Yunxia;Tang, Zhiru;Li, Tiejun;Chen, C.;Huang, Feiruo;...

期刊： JOURNAL OF ANIMAL SCIENCE,2018年96(9):3804-3814 ISSN：0021-8812

通讯作者： Sun, Zhihong

作者机构： [Li, Yunxia] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.;[An, Rui; Sun, Jiajing; Zhen, Jifu; Wu, Zhaoliang; Li, Mao; Wu, Liuting; Sun, Zhihong; Li, Yunxia; Tang, Zhiru; Xu, Qingqing; Zhang, Xiangxin; Yang, Jing; Chen, Jinchao; Chen, C.] Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.;[Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Huang, Feiruo] Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Zhao, Shengjun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Sun, Zhihong] S;Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.

关键词： alanine;amino acid;glutamic acid;nitrogen;pyruvic acid;urea;animal;animal food;cell line;cytology;diet;dietary supplement;drug effect;energy metabolism;epithelium cell;intestine mucosa;male;metabolism;oxidation reduction reaction;physiology;pig;protein intake;protein restriction;veterinary medicine;Alanine;Amino Acids;Animal Feed;Animal Nutritional Physiological Phenomena;Animals;Cell Line;Diet;Diet, Protein-Restricted;Dietary Proteins;Dietary Supplements;Energy Metabolism;Epithelial Cells;Glutamic Acid;Intestinal Mucosa;Male;Nitrogen;Oxidation-Reduction;Pyruvic Acid;Swine;Urea

摘要： This study was performed to determine if pyruvate, which acts as a critical intermediate in energy metabolism, can substitute the role of glutamate as a metabolic fuel and effectively reduce nitrogen excretion in pigs. First, the experiment in vitro was carried out to investigate the effects of culturing porcine small intestinal epithelial cell line with pyruvate on the oxidation. Then, barrows weighing 40 kg were used in the experiment investigating the changes of nitrogen balance in response to addition of pyruvate to low-protein diets. Last, barrows (40 kg), which were surgically fitted with permanent catheters in the mesenteric vein, portal vein, hepatic vein, and carotid artery, were used to investigate the effects of supplementing low-protein diets with calcium pyruvate on the net portal fluxes of amino acids (AAs) and the consumption of AAs in the liver. The results showed that culturing cells with sodium pyruvate significantly reduced the number of glutamate oxidation (P < 0.05). Addition of calcium pyruvate to low-protein diets significantly reduced urinary nitrogen excretion from 13.2 g/d (18.0% crude protein, CP) to 10.3 g/d (15.0% CP) or 7.80 g/d (13.5% CP) and total nitrogen excretion from 22.5 g/d (18.0% CP) to 17.8 g/d (15.0% CP) or 14.2 g/d (13.5% CP) (P < 0.05), without obviously negative effects on the nitrogen retention (P > 0.05). Addition of calcium pyruvate to low-protein diets significantly decreased essential AA consumption rate in the liver (P < 0.05). This diet modification reduced the net portal fluxes of NH3, glycine, and alanine, as well as urea production rate in the liver (P < 0.05). The results indicated that pyruvate is an effective substitute for glutamate as a supplement in low-protein diets, reducing porcine nitrogen excretion and nitrogen consumption. © The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved.

语种： 英文

作者： 邹红;李灿;汪润秋;李文祥;吴山功;...

期刊： 水生生物学报,2018年42(2):364-368 ISSN：1000-3207

作者机构： 中国科学院水生生物研究所, 农业部淡水养殖病害防治重点实验室;[邹红; 汪润秋; 李文祥; 吴山功; 王桂堂; 李明] 淡水生态与生物技术国家重点实验室, 武汉, 430072;武汉轻工大学动物科学与营养工程学院, 武汉, 430023;[邹红; 汪润秋; 李文祥; 吴山功; 王桂堂; 李明] 中国科学院水生生物研究所, 农业部淡水养殖病害防治重点实验室;[李灿] 武汉轻工大学动物科学与营养工程学院, 武汉, 430023

关键词： 蛙片虫;原蛙片虫属;形态学;泽蛙

摘要： 对采自湖北省洪湖地区的泽蛙(Fejervarya limnocharis)肠道内寄生的泽蛙原蛙片虫进行了形态学再描述,包括活体形态、固定染色标本形态及扫描电镜下超微结构。样本形态特征参数值与Nie(倪达书)首次发现并命名时所记述标本参数值颇为相符(包括体长、体宽,核长、核宽)。此外,本文对2个重要的分类特征进行了补充和修正:(1)缝线位于虫体顶端,贯穿背腹侧;所有体动基列均从缝线两侧发出。(2)胞核分裂时在两新核间产生的连接细丝,在虫体整个生活周期一直存在。并针对这2点特征与其他已知的原蛙片虫属种类进行了比较和讨论:认为缝线结构是较为稳定的分类特征之一,体动基列均由此处生发并与虫体纵轴平行延伸;推测泽蛙原蛙片虫子代虫体中的2个胞核均来自于母体中同一个核,而其2核间的连接细丝可能是胞核分裂进化历程中比较原始的残迹。

语种： 中文

作者： Wu, Liuting;Zhang, Xiangxin;Tang, Zhiru;Li, Yunxia;Li, Tiejun;...

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018年66(31):8262-8271 ISSN：0021-8561

通讯作者： Sun, Zhihong;Yin, Yulong;Jiang, Qingyan;Zhu, Weiyun

作者机构： [An, Rui; Sun, Jiajing; Zhen, Jifu; Wu, Zhaoliang; Li, Mao; Wu, Liuting; Sun, Zhihong; Tang, Zhiru; Xu, Qingqing; Zhang, Xiangxin; Chen, Cheng; Yang, Jing; Chen, Jinchao] Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.;[Li, Yunxia] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.;[Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Huang, Feiruo] Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Zhao, Shengjun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Sun, Zhihong] S;[Yin, Yulong] C;[Jiang, Qingyan] H;[Zhu, Weiyun] N;Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.

关键词： Ascorbic acid;Liver;Metabolism;Nitrogen;Proteins;Urea;Carotid artery;Crude proteins;Hepatic veins;Low protein diet;Nitrogen balance;Nitrogen excretion;Nitrogen retention;Urea production;Amino acids;amino acid;lysine;methionine;nitrogen;threonine;tryptophan;urea;animal;chemistry;diet;dietary supplement;feces;liver;male;metabolism;pig;protein restriction;urine;veterinary;viscera;Amino Acids;Animals;Diet;Diet, Protein-Restricted;Dietary Supplements;Feces;Liver;Lysine;Male;Methionine;Nitrogen;Sus scrofa;Threonine;Tryptophan;Urea;Viscera

摘要： Reducing dietary crude protein (CP) intake effectively decreases nitrogen excretion in growing-finishing pigs but at the expense of poor growth when dietary CP content is reduced by ≥3%. In this study, we investigated the main disadvantages of low-protein diets supplemented with lysine, methionine, threonine, and tryptophan in pigs. First, changes in the nitrogen balance in response to differences in dietary CP content (18%, 15%, and 13.5%) were investigated in barrows (40 kg). Then, barrows (40 kg) surgically fitted with catheters in the mesenteric vein, portal vein, hepatic vein, and carotid artery were used to investigate changes in amino acid (AA) metabolism in the portal-drained viscera and liver in response to differences in dietary CP content. The results showed that low-protein diets reduced fecal and urinary nitrogen excretion (P &lt; 0.05) meanwhile resulted in significant decreases in nitrogen retention (P &lt; 0.05). Moreover, a reduction in the dietary CP content from 18% to 13.5% resulted in decreases in the net portal fluxes of NH3, glycine, and alanine as well as in the urea production in the liver (P &lt; 0.05), whereas their values as a percentage of nitrogen intake did not decline (P &gt; 0.05). The net portal fluxes of nonessential AA (NEAA) were reduced in the low-protein diet groups (P &lt; 0.05), while essential AA consumption in the liver increased (P &lt; 0.05). Thus, low-protein diets result in reductions in both nitrogen excretion and retention, and NEAA deficiency may be a major disadvantage of low-protein diets. © 2018 American Chemical Society.

语种： 英文

作者： Li, Can;Zhao, Weishan;Zhang, Dong;Wang, Runqiu;Wang, Guitang;...

期刊： PARASITE,2018年25:38 ISSN：1252-607X

通讯作者： Li, Ming

作者机构： [Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Can; Li, Wenxiang] Chinese Acad Sci, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China.;[Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Can; Li, Wenxiang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.;[Li, Can] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Li, Ming] C;Chinese Acad Sci, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China.;Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.

关键词： animal;Anura;ciliate;classification;genetics;isolation and purification;parasitology;phylogeny;RNA gene;scanning electron microscopy;species difference;ultrastructure;Animals;Anura;Ciliophora;Genes, rRNA;Microscopy, Electron, Scanning;Phylogeny;Species Specificity

摘要： Morphological studies of Sicuophora (Syn. Wichtermania) multigranularis Xiao et al., 2002, from the rectum of the frog, Quasipaa spinosa, performed using silver impregnation and scanning electron microscopy, confirmed the following newly recognized features: (1) only one apical suture on the right surface; (2) two naked regions at the posterior end of both the left and the right side of the body. Phylogenetic analysis based on the SSU-rRNA gene showed that S. multigranularis is a sister to a clade comprising all other Clevelandellida, strongly supporting the validity of the genus Sicuophora. This is also the first molecular data obtained for the genus Sicuophora. Because of the lack of molecular data, it will be necessary to obtain more genetic data from the family Sicuophoridae to discuss the question of the taxonomic status of the genus Sicuophora. SSU-rRNA. © 2018 EDP Sciences.

语种： 英文

作者： Rui An;Tang, Zhiru;Li, Yunxia;Li, Tiejun;Xu, Qingqing;...

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018年66(15):3793-3800 ISSN：0021-8561

通讯作者： Sun, Zhihong;Yin, Yulong;Jiang Qingyan;Zhu, Weiyun

作者机构： [Sun, Jiajing; Zhen, Jifu; Wu, Zhaoliang; Rui An; Jing Yang; Wu, Liuting; Sun, Zhihong; Mao Li; Tang, Zhiru; Xu, Qingqing; Zhang, Xiangxin; Cheng Chen; Chen, Jinchao] Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.;[Li, Yunxia] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.;[Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Huang, Feiru] Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Zhao, Shengjun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Sun, Zhihong] S;[Yin, Yulong] C;[Jiang Qingyan] H;[Zhu, Weiyun] N;Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.

关键词： Amino acids;Ascorbic acid;Bacteria;Cells;Chemical activation;Cytology;Dichloroacetic acid;Mammals;Metabolism;Nutrients;Phosphorylation;Physiology;Sodium compounds;-glutamate;Dichloroacetate;Glucose consumption;Nutrient consumption;Oxidative metabolism;Pyruvate dehydrogenase kinase;Pyruvates;Small interfering rna (siRNA);Glucose;amino acid;dichloroacetic acid;glucose;pyruvate dehydrogenase complex;pyruvic acid derivative;analysis;animal;bacterium;cell line;dietary supplement;drug effects;intestine;metabolism;microbiology;pig;Amino Acids;Animals;Bacteria;Cell Line;Dichloroacetic Acid;Dietary Supplements;Glucose;Intestines;Pyruvate Dehydrogenase Complex;Pyruvates;Swine

摘要： The extensive metabolism of amino acids (AA) as fuel is an important reason for the low use efficiency of protein in pigs. In this study, we investigated whether regulation of the pyruvate dehydrogenase kinase (PDK)/pyruvate dehydrogenase alpha 1 (PDHA1) pathway affected AA consumption by porcine intestinal epithelial (IPEC-J2) cells and intestinal bacteria in pigs. The effects of knockdown of PDHA1 and PDK1 with small interfering RNA (siRNA) on nutrient consumption by IPEC-J2 cells were evaluated. IPEC-J2 cells were then cultured with sodium dichloroacetate (DCA) to quantify AA and glucose consumption and nutrient oxidative metabolism. The results showed that knockdown of PDHA1 using siRNA decreased glucose consumption but increased total AA (TAA) and glutamate (Glu) consumption by IPEC-J2 cells (P < 0.05). Opposite effects were observed using siRNA targeting PDK1 (P < 0.05). Additionally, culturing IPEC-J2 cells in the presence of 5 mM DCA markedly increased the phosphorylation of PDHA1 and PDH phosphatase 1, but inhibited PDK1 phosphorylation (P < 0.05). DCA treatment also reduced TAA and Glu consumption and increased glucose depletion (P < 0.05). These results indicated that PDH was the regulatory target for shifting from AA metabolism to glucose metabolism and that culturing cells with DCA decreased the consumption of AAs by increasing the depletion of glucose through PDH activation. © 2018 American Chemical Society.

语种： 英文

作者： Zhao, Weishan;Li, Can;Zhang, Dong;Wang, Runqiu;Zheng, Yingzhen;...

期刊： PARASITE,2018年25:29 ISSN：1252-607X

通讯作者： Li, Ming

作者机构： [Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Wenxiang] Chinese Acad Sci, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Hubei, Peoples R China.;[Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Wenxiang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.;[Li, Can] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Dong; Wang, Runqiu; Zhao, Weishan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Zheng, Yingzhen] Agr Forestry Anim Husb & Aquaculture Bur Guye Dis, Anim Husb & Aquaculture Stn, Tangshan 063100, Peoples R China.

通讯机构： [Li, Ming] C;Chinese Acad Sci, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Hubei, Peoples R China.;Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.

关键词： protozoal DNA;ribosome DNA;anatomy and histology;animal;Anura;Balantidium;China;classification;genetics;isolation and purification;microscopy;parasitology;phylogeny;rectum;scanning electron microscopy;ultrastructure;Animals;Anura;Balantidium;China;DNA, Protozoan;DNA, Ribosomal;Microscopy;Microscopy, Electron, Scanning;Phylogeny;Rectum

摘要： Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is "V"-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.

语种： 英文

作者： Sun, Zongyi;Jiang, Chuanqi;Feng, Jinmei;Yang, Wentao;Li, Ming*;...

期刊： PARASITE,2017年24:43 ISSN：1252-607X

通讯作者： Li, Ming;Miao, Wei

作者机构： [Li, Ming; Sun, Zongyi; Li, M; Miao, W] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Li, Ming; Yang, Wentao; Li, M; Miao, W; Jiang, Chuanqi; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.;[Feng, Jinmei] Jianghan Univ, Sch Med, Dept Pathogen Biol, Wuhan 430056, Hubei, Peoples R China.

通讯机构： [Li, M; Miao, W] W;[Li, M; Miao, W] C;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.

关键词： protozoal DNA;ribosome DNA;algorithm;animal;balantidiasis;Balantidium;Bayes theorem;carp;chemistry;China;classification;fish disease;fishery;gene expression profiling;genetics;Markov chain;Monte Carlo method;nucleotide sequence;parasitology;phylogeny;procedures;sequence alignment;sequence analysis;statistical model;veterinary;Algorithms;Animals;Balantidiasis;Balantidium;Base Sequence;Bayes Theorem;Carps;China;DNA, Protozoan;DNA, Ribosomal;Fish Diseases;Fisheries;Gene Expression Profiling;Likelihood Functions;Markov Chains;Monte Carlo Method;Phylogeny;Sequence Alignment;Sequence Analysis, RNA

摘要： In this paper, we present transcriptome data for Balantidium ctenopharyngodoni Chen, 1955 collected from the hindgut of grass carp (Ctenopharyngodon idella). We evaluated sequence quality and de novo assembled a preliminary transcriptome, including 43.3 megabits and 119,141 transcripts. Then we obtained a final transcriptome, including 17.7 megabits and 35,560 transcripts, by removing contaminative and redundant sequences. Phylogenomic analysis based on a supermatrix with 132 genes comprising 53,873 amino acid residues and phylogenetic analysis based on SSU rDNA of 27 species were carried out herein to reveal the evolutionary relationships among six ciliate groups: Colpodea, Oligohymenophorea, Litostomatea, Spirotrichea, Heterotrichea and Protocruziida. The topologies of both phylogenomic and phylogenetic trees are discussed in this paper. In addition, our results suggest that single-cell sequencing is a sound method of obtaining sufficient omics data for phylogenomic analysis, which is a good choice for uncultivable ciliates. The transcriptome data for Balantidium ctenopharyngodoni are the first omics data within the subclass Trichostomatia, and provide a good basis for ciliate phylogenomic analysis, as well as related omics analysis.

语种： 英文

作者： Li, Can;Jin, Xiao;Li, Ming*;Wang, Guitang;Zou, Hong;...

期刊： PARASITE,2017年24:6 ISSN：1252-607X

通讯作者： Li, Ming

作者机构： [Jin, Xiao; Li, Can] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Zou, Hong; Li, Ming; Wu, Shangong; Wang, Guitang; Li, Wenxiang] Chinese Acad Sci, Inst Hydrobiol, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Peoples R China.

通讯机构： [Li, Ming] C;Chinese Acad Sci, Inst Hydrobiol, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Peoples R China.

关键词： animal;Anura;infection;microbiology;parasitology;stramenopile;transmission electron microscopy;ultrastructure;veterinary;Animals;Anura;Infection;Microscopy, Electron, Transmission;Stramenopiles

摘要： Cepedea longa Bezzenberger, 1904, collected from Fejervarya limnocharis (Amphibia, Anura, Ranidae) from Honghu Lake, Hubei Province, China in May-July 2016, is described at both light and transmission electron microscope levels. This is the first electron microscopic study of this species. Cepedea longa possesses a developed fibrillar skeletal system, composed of longitudinal fibrillar bands and transversal fibrils as well as numerous thin microfibrils dispersed in the endoplasm, which may play an important role in morphogenesis and offer some resilience to deformations of the cell. Longitudinal microfibrils are polarizing elements of kineties, bordering the somatic kineties on the left side and possibly responsible for kinetosome alignment. Two types of vesicles exist in the somatic cortex: globular endocytotic vesicles and flattened exocytotic vesicles. As to the nuclei of C. longa, a thick microfibrillar layer was observed to attach to the cytoplasmic face of the nuclear envelope. This fact suggests no necessary connection between the presence of this microfibrillar layer and the number of nuclei. In addition, some unknown tightly-packed microtubular structures in the nucleoplasm were observed for the first time in opalinids; neither their nature nor physiological significance is known. A detailed list of all reported Cepedea species is included. © C. Li et al., published by EDP Sciences, 2017.

语种： 英文

作者： Li, Ming;Li, Can;Grim, J. Norman;Ponce-Gordo, Francisco;Wang, Guitang*;...

期刊： European Journal of Protistology,2017年58:152-163 ISSN：0932-4739

通讯作者： Wang, Guitang

作者机构： [Zou, Hong; Li, Ming; Wu, Shangong; Wang, Guitang; Li, Wenxiang] Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei Province, Peoples R China.;[Li, Can] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Grim, J. Norman] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA.;[Ponce-Gordo, Francisco] Univ Complutense Madrid, Fac Farm, Dept Parasitol, Plaza Ramon y Cajal S-N, E-28040 Madrid, Spain.;[Li, Ming] Hubei Collaborat Innovat Ctr Freshwater Aquacultu, Wuhan 430070, Peoples R China.

通讯机构： [Wang, Guitang] C;Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei Province, Peoples R China.

关键词： Macrocytopharynxa;Morphology;Nyctotheroides;Nyctotherus;Phylogenetic analysis;SSU-rDNA

摘要： The morphological revisions of Macrocytopharynxa pyriformis (Nie, 1932) Li et al., 2002; collected from the rectum of Fejervarya limnocharis (=Rana limnocharis), are presented in this paper: (1) two surfaces of the organism are not - identical left side narrower and convex, right broader and flat or slightly concave; (2) infundibulum is large and well-developed with no "fold" or "plicature" present in the middle or posterior portion; (3) micronucleus is tiny and ovoid shaped and always embedded in the middle concavity of macronucleus, which can be well revealed by ammoniacal silver staining. Our phylogenetic analysis based on SSU-rDNA showed that M. pyriformis fell into the Nyctotheroides Glade, within which four definite Nyctotheroides species were involved - N. cordiformis, N. deslierresae, N. parvus and N. hubeiensis. In combination with their morphological features, we discussed the reliability of using karyophore organelles or kinetal suture patterns as the generic taxonomic criteria. Besides, we considered that the genus Macrocytopharynxa is a junior synonym of Nyctotheroides and we transfer its type species to Nyctotheroides as Nyctotheroides pyriformis n. comb. The phylogenetic pattern of the family Nyctotheridae was also indicated in our work, but it will be necessary to analyze more species from fishes and reptiles before coming to a sound conclusion. (C) 2016 Elsevier GmbH. All rights reserved.

语种： 英文

作者： Wuming Yang;Ningqiu Li;Ming Li;Defeng Zhang;Guannan An

期刊： Genome Announcements,2016年4(1) ISSN：2169-8287

通讯作者： Li, Ningqiu

作者机构： [Ming Li; Guannan An; Wuming Yang] School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, China;[Ningqiu Li] Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, China liningq@126.com;[Defeng Zhang] Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, China

通讯机构： [Li, Ningqiu] P;Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, China

摘要： <jats:title>ABSTRACT</jats:title> <jats:p> <jats:named-content content-type="genus-species">Aeromonas hydrophila</jats:named-content> is one of the most important fish pathogens in China. Here, we report complete genome sequence of a virulent strain, <jats:italic>A. hydrophila</jats:italic> JBN2301, which was isolated from diseased crucian carp. </jats:p>

语种： 英文

作者： Ming Li;Zongyi Sun;Chong Wang*;Lin Zhang

期刊： Journal of Parasitic Diseases,2016年40(4):1429-1434 ISSN：0971-7196

通讯作者： Chong Wang

作者机构： [Sun Z.] Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China;[Wang C.] Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, 430079, China;[Zhang L.] Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan, 430223, China;Hubei Collaborative Innovation Centre for Freshwater Aquaculture, Wuhan, 430070, China;[Li M.] Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Hubei Collaborative Innovation Centre for Freshwater Aquaculture, Wuhan, 430070, China

通讯机构： [Chong Wang] I;Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, China

关键词： animal experiment;Apiosoma piscicola;Article;China;Chordata;ciliate;gill;mortality;nonhuman;priority journal;scanning electron microscopy;Tachysurus fulvidraco;transmission electron microscopy

摘要： This paper gave detailed description of the fine structure of Apiosoma piscicola Blanchard 1885. These parasites were collected from the fry of Tachysurus fulvidraco during parasite surveys of fish at Jiangxia Fish Hatchery in Wuhan, Hubei province in May 2011. Its pellicle, peristomial apparatus, nuclei, trochal band and scopula were described, respectively. The relationship between these peritrich ciliates and their hosts was well discussed herein. The scopula of A. piscicola did not adhere directly to the surface of the host epithelial cells, but by means of a secreted adhesive pad of material according to the present study. Besides, no ultrastructural damage to host epithelial cells could be detected in our work. We concluded that these sessilids should be ectocommensals rather than ectoparasites, but not always harmless. In addition, their great variability of body shape and non-strict host specificity were also discussed. © 2016, Indian Society for Parasitology.

语种： 英文

作者： Wang, Chong;Liang, Yin-Quan*;Li, Ming*;Zhang, Yu;Shen, Zai-Jun;...

期刊： Mitochondrial DNA Part A,2016年27(6):4617-4619 ISSN：2470-1394

通讯作者： Liang, Yin-Quan;Li, Ming

作者机构： [Liang, Yin-Quan; Wang, Chong] Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan, Peoples R China.;[Liang, Yin-Quan; Wang, Chong] Chinese Acad Sci, Wuhan, Peoples R China.;[Li, Ming] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Zhang, Yu; Shen, Zai-Jun; Jiang, Zhi-Wu] Jin Sha River Ludila Hydropower Co, Kunming, Peoples R China.

通讯机构： [Liang, Yin-Quan] M;[Liang, Yin-Quan] C;[Li, Ming] W;Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan, Peoples R China.;Chinese Acad Sci, Wuhan, Peoples R China.

关键词： genomic DNA;mitochondrial DNA;ribosome RNA;transfer RNA;fish protein;mitochondrial DNA;ribosome RNA;transfer RNA;Article;Botiidae;conservation genetics;controlled study;Cypriniformes;DNA extraction;DNA sequence;mitochondrial genome;molecular systematics;Myxocyprinus asiaticus;Nemaeheilidae;nonhuman;phylogenetic tree;polymerase chain reaction;priority journal;sequence analysis;species identification;Triplophysa venusta;animal;chemistry;classification;Cypriniformes;DNA base composition;genetics;isolation and purification;metabolism;open reading frame;phylogeny;Animals;Base Composition;Cypriniformes;DNA, Mitochondrial;Fish Proteins;Genome, Mitochondrial;Open Reading Frames;Phylogeny;RNA, Ribosomal;RNA, Transfer;Sequence Analysis, DNA

摘要： The complete mitochondrial DNA sequence of Triplophysa venusta was determined using a PCR-based method in our present study. The genome is totally 16 574 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA, 22 transfer RNA genes, and 1 non-coding control region. The overall base composition of T. venusta is A 27.8%, T 26.9%, C 26.9%, and G 18.4%, with an A + T bias of 54.7%. This complete mitogenome of T. venusta provides a basic data for studies on species identification, molecular systematics, and conservation genetics. The phylogenetic trees consistently showed that the Family Cobitidae was divided into three major clades: the subfamily Botiidae, the subfamily Nemaeheilidae, and the subfamily Cobitinae. Cobitinae and Nomaeheilidae formed a main Loach clade, with Botiidae clustered at the basal. © 2015 Taylor & Francis.

语种： 英文

作者： Li, Ming;Sun, Zong-Yi;Grim, J. Norman;Ponce-Gordo, Francisco*;Wang, Gui-tang;...

期刊： JOURNAL OF EUKARYOTIC MICROBIOLOGY,2016年63(6):751-759 ISSN：1066-5234

通讯作者： Ponce-Gordo, Francisco

作者机构： [Li, Wen-xiang; Zou, Hong; Wu, Shan-gong; Li, Ming; Wang, Gui-tang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.;[Sun, Zong-Yi] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Grim, J. Norman] No Arizona Univ, Dept Biol Sci, Box 5640, Flagstaff, AZ 86011 USA.;[Ponce-Gordo, Francisco] Univ Complutense Madrid, Fac Farm, Dept Parasitol, Plaza Ramon y Cajal S-N, E-28040 Madrid, Spain.

通讯机构： [Ponce-Gordo, Francisco] U;Univ Complutense Madrid, Fac Farm, Dept Parasitol, Plaza Ramon y Cajal S-N, E-28040 Madrid, Spain.

关键词： Light microscopy;Nyctotheroides;phylogenetic analysis;SSU-rDNA

摘要： The morphology of Nyctotheroides hubeiensis (Acta Hydrobiol. Sin. 1998, 22(suppl.):187), collected from the rectum of Phelophylax nigromaculatus, is presented in this paper based on detailed morphological information and molecular data. Our phylogenetic analysis showed that N.hubeiensis fell into the Nyctotheroides clade, which was strongly supported as monophyletic and clustered as basal to the genera Nyctotherus and Clevelandella. Also, the monophyly of the Order Clevelandellida and the affinity of parasitic nyctotherids and free-living metopids were indicated in our work. The origin of clevelandellid ciliates as well as their possible evolutionary history was also discussed here; however, the analysis of more species from other vertebrate hosts (fish, reptiles) should be made before a well-supported conclusion can be drawn. © 2016 The Author(s) Journal of Eukaryotic Microbiology © 2016 International Society of Protistologists

语种： 英文

作者： Li, Weidong;Wang, Chong;Huang, Feng;Li, Ming*;Nilsen, Frank;...

期刊： PARASITE,2014年21:46 ISSN：1252-607X

通讯作者： Li, Ming

作者机构： [Li, Ming; Huang, Feng; Li, Weidong; Xu, Jianlong; Liu, Huiyu] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wang, Chong] Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.;[Wang, Chong] Chinese Acad Sci, Wuhan 430079, Peoples R China.;[Li, Ming; Nilsen, Frank] Univ Bergen, Dept Biol, Sea Lice Res Ctr, N-5020 Bergen, Norway.;[Li, Ming] Hubei Collaborat Innovat Ctr Freshwater Aquacultu, Wuhan 430070, Peoples R China.

通讯机构： [Li, Ming] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

关键词： animal;China;classification;isolation and purification;parasitology;Ranidae;rectum;scanning electron microscopy;species difference;stramenopile;symbiosis;ultrastructure;Animals;China;Microscopy, Electron, Scanning;Ranidae;Rectum;Species Specificity;Stramenopiles;Symbiosis

摘要： A redescription of Protoopalina pingi Nie, 1935 is presented in this paper to complete Nie's description at both light and scanning electron microscope levels. These organisms were collected from the recta of the frogs Hylarana guentheri Boulenger, 1882 and Pelophylax nigromaculatus Hallowell, 1861 from Jialing River, Sichuan Province and Honghu Lake, Hubei Province, respectively, in China. This is the first record of its occurrence in H. guentheri and P. nigromaculatus. The body of P. pingi is elongated and somewhat spindle-like in shape, slightly narrowed and bluntly rounded at the anterior extremity, while the posterior end is tapering or sharply pointed. The body surface is thickly flagellated, with the caudal tip being barren. The falx, located at the margin of the anterior end, is composed of a narrow band of kinetosomes. Four round or oval-shaped nuclei, usually arranged in a straight line, are situated in the middle region of the body. Comparisons are made between P. pingi and its congeners. © 2014 W. Li et al., published by EDP Sciences.

语种： 英文

作者： Li, Ming;Ponce-Gordo, Francisco;Grim, J. Norman;Wang, Chong*;Nilsen, Frank

期刊： Parasitology Research,2014年113(12):4327-4333 ISSN：0932-0113

通讯作者： Wang, Chong

作者机构： [Li, Ming] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wang, Chong] Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.;[Wang, Chong] Chinese Acad Sci, Wuhan 430079, Peoples R China.;[Li, Ming; Nilsen, Frank] Univ Bergen, Dept Biol, Sea Lice Res Ctr, N-5020 Bergen, Norway.;[Ponce-Gordo, Francisco] Univ Complutense Madrid, Fac Farm, Dept Parasitol, E-28040 Madrid, Spain.

通讯机构： [Wang, Chong] M;Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.

关键词： small subunit ribosomal RNA;protozoal DNA;ribosome DNA;Article;Balantidium;Balantidium ctenopharingodoni;Balantidium polyvacuolum;cladistics;controlled study;fish;host parasite interaction;molecular phylogeny;morphological trait;nonhuman;nucleotide sequence;phylogenetic tree;polyphyly;priority journal;RNA sequence;species identification;taxonomic identification;animal;balantidiasis;Balantidium;chemistry;classification;DNA sequence;fish disease;genetics;isolation and purification;molecular genetics;parasitology;phylogeny;small ribosomal subunit;veterinary;Animals;Balantidiasis;Balantidium;Base Sequence;DNA, Protozoan;DNA, Ribosomal;Fish Diseases;Fishes;Molecular Sequence Data;Phylogeny;Ribosome Subunits, Small;Sequence Analysis, DNA

摘要： We obtained sequences of the small subunit ribosomal RNA (rRNA) for two new isolates of Balantidium from fishes, Balantidium polyvacuolum and Balantidium ctenopharingodoni. This is the first introduction of molecular data of Balantidium species from fish hosts in the phylogenetic analyses of the ciliate subclass Trichostomatia. Despite the fact that these species share morphological characteristics common to other species of Balantidium, the phylogenetic analysis of their sequences has shown that they are to be placed in a different branch closely related to the so-called Australian clade. Thus, our results indicate that the genus Balantidium is polyphyletic and possibly should be represented by two different genera; however, the analysis of more species from other poikilothermic hosts (amphibians, reptiles) should be made before a revised taxonomical proposal could be made. © 2014, Springer-Verlag Berlin Heidelberg.

语种： 英文

作者： Li Ming;Wang Chong*;Grim, J. Norman;Wang Jianguo;Gong Xiaoning

期刊： Acta Parasitologica,2014年59(3):413-419 ISSN：1230-2821

通讯作者： Wang Chong

作者机构： [Li Ming] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wang Chong] Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.;[Wang Chong] Chinese Acad Sci, Wuhan 430079, Peoples R China.;[Grim, J. Norman] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA.;[Wang Jianguo; Gong Xiaoning] Chinese Acad Sci, Inst Hydrobiol, Lab Hlth Aquaculture, Wuhan 430072, Peoples R China.

通讯机构： [Wang Chong] M;Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.

关键词： Ultrastructure;ciliates;Balantidium ctenopharyngodoni;vestibulum;microtubular ribbons

摘要： A detailed description of the fine structure of Balantidium ctenopharyngodoni Chen, 1955 with an emphasis on its vestibulum is given in the present paper. As to the vestibular kinetids, special attention is paid to the characters of T1, T2 microtubules and nematodesmata. Serving as the major skeleton to the vestibular cortex, the T1, T2 and Pc microtubules are described herein and their support function is also discussed. Moreover, the well-developed nematodesmata of the vestibular kinetids that form a large basket-like complex are described in detail.

语种： 英文

作者： Ming Li;Zongyi Sun;Chong Wang

期刊： Journal of Veterinary Parasitology,2014年28(1):56-61 ISSN：0971-6157

作者机构： [Wang C.] Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, 430079, China;Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China;Hubei Collaborative Innovation Centre for Freshwater Aquaculture, Wuhan, 430070, China;[Li M.] Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, 430079, China<&wdkj&>Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Hubei Collaborative Innovation Centre for Freshwater Aquaculture, Wuhan, 430070, China;[Sun Z.] Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, 430079, China<&wdkj&>Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China

关键词： Apiosoma piscicola;Apiosoma piscicola infection;Article;cell organelle;China;cytoplasm;ectoparasitosis;heterochromatin;host range;kinetosome;macronucleus;microscopy;nonhuman;parasite;parasite identification;Pelteobagrus fulvidraco;scanning electron microscopy;transmission electron microscopy;ultrastructure

摘要： This paper gave detailed description of the fine structure of Apiosoma piscicola Blanchard 1885 occurring on fry of Pelteobagrus fulvidraco during parasite surveys of fish at Jiangxia Fish Hatchery in Wuhan, Hubei province in May 2011. Its pellicle, peristomial apparatus, nuclei, transverse ciliary band and scopula were described respectively. The relationship between these peritrich ciliates and their hosts are well discussed herein. The scopula of A. piscicola did not adhere directly to the surface of the host epithelial cells, but by means of a secreted adhesive pad of material. Besides, no ultrastructural damage to host epithelial cells could be detected. It is concluded that these sessilids are ectocommensals rather than ectoparasites and are not always harmless. In addition, their great variability and non-strict host specificity is also discussed. © IAAVP, India.

语种： 英文

作者： Li, Ming;Li, Weidong;Zhang, Lei;Wang, Chong*

期刊： Korean Journal of Parasitology,2013年51(4):427-431 ISSN：0023-4001

通讯作者： Wang, Chong

作者机构： [Li, Ming; Li, Weidong; Zhang, Lei] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Li, Ming; Wang, Chong] Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.;[Li, Ming; Wang, Chong] Chinese Acad Sci, Wuhan 430079, Peoples R China.;[Li, Ming] Hubei Collaborat Innovat Ctr Freshwater Aquacultu, Wuhan 430070, Peoples R China.

通讯机构： [Wang, Chong] M;Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.

关键词： Anura;article;Balantidium;Balantidium honghuensis;body height;body size;cell vacuole;China;microbial morphology;morphometrics;new species;nonhuman;Rana limnocharis;Rana nigromaculata;scanning electron microscopy;species identification;vestibule;Balantidium honghuensis;China;frog;Rana limnocharis;Rana nigromaculata;trichostome ciliate;Animals;Balantidiasis;Balantidium;China;Ranidae;Rectum

摘要： A new trichostome ciliate, Balantidium honghuensis n. sp., was isolated from the recta of Rana nigromaculata and R. limnocharis during parasite surveys in Honghu Lake, Hubei Province, central China in summer of 2010 and 2011. Its detailed morphometric characters based on LM and SEM studies were described herein. The organism is oval in shape and thickly ciliated. The vestibulum is ""V"" shaped and occupies about 1/3 to 2/5 of the body length. The vestibular and nearby regions possess strong peripheral fibers which form a marked axial fiber about the cytopharynx. More than 10 contractile vacuoles are distributed along the periphery of the latter body. Comparisions were made between this new species and B. sinensis Nie, 1935. They were discriminated from each other in terms of general body forms, body size, and vestibulum shapes. Besides, special attention was paid to its high-speed daughter swarmers which we believed to be the infective stage of B. honghuensis. Possible infection routes of anura amphibian balantidia were discussed. © 2013, Korean Society for Parasitology and Tropical Medicine.

语种： 英文

作者： 李明;李伟东;戈贤平;汪建国;龚小宁;...

作者机构： [李明; 李伟东; 戈贤平; 汪建国; 龚小宁; 黄峰; 刘辉宇] 武汉工业学院湖北省动物营养与饲料科学重点实验室;[李明; 李伟东; 戈贤平; 汪建国; 龚小宁; 黄峰; 刘辉宇] 中国水产科学研究院淡水渔业研究中心;[李明; 李伟东; 戈贤平; 汪建国; 龚小宁; 黄峰; 刘辉宇] 中国科学院水生生物研究所

会议名称： 2013年中国水产学会鱼病专业委员会学术研讨会

会议时间： 2013-11-01

会议地点： 海口

会议论文集名称： 2013年中国水产学会鱼病专业委员会学术研讨会论文集

关键词： 珠蚌累枝虫;黄颡鱼鱼苗;缘毛亚纲;皮肤损伤

摘要： 累枝虫（Epistylis sp.）隶属于缘毛亚纲,固着目,累枝虫科;是一类广泛附生于多种水生植物、无脊椎动物和脊椎动物的纤毛虫。在一定情况下,可以严重危害养殖的鱼苗、鱼种、鳖及各种虾、蟹的卵、幼体和成体。其中尤以对虾、蟹的幼体和鱼苗的危害最大,可引起大批死亡,残存的商品价值也大大降低。珠蚌累枝虫（Epistylis unioi）是龚循矩（1986）在圆顶珠蚌的鳃叶上发现并命名的。本文以寄生在黄颡鱼鱼苗的珠蚌累枝虫为主要研究对象,利用蛋白银染色法和扫描电镜技术,对这一虫种进行了再描述。珠蚌累枝虫在黄颡鱼苗体表的发现是其宿主的一个新纪录。结果表明:黄颡鱼鱼苗为大量的珠蚌累枝虫感染,鱼体表现为生命力减弱,游动缓慢,浮头、呼吸急促;置于解剖镜下观察发现大量的虫体存在于鱼体体表、鳍、须和鳃上。虫体细长,略呈花瓶状,活体状态下虫体完全伸展时体长56-88（68）μm,体宽22-38（30）μm,口围唇的直径27-45（35）μm。蛋白银染色状态下,体长34-53（41）μm,体宽16-26（22）μm。虫体表面可见多条横向排列的条纹,在口围与横纤毛带间有37-45（41）条,横纤毛带与帚胚间有26-33（29）条,相邻条纹间距约为0.5μm。横向条纹上可见许多排列稀疏的气孔。单毛基列和复毛基列均起自于细胞顶端口围唇的内侧,两者相伴而行,进入口区之前围绕口缘1.5圈。P1-3分别由三条毛基索组成,其中P1、P2远长于P3;P1的下端一直延伸至胞口处,而P2和P3末端交汇,位于P1的下端点之上。伸缩泡一个,位于虫体顶端口围盘稍下处。大核呈马蹄形,横位,位于虫体的前部。群体较小,呈不对称二叉式分支。此外,我们还发现了虫体的游泳体,在横纤毛带上生出密集的纤毛。扫描电镜显示,在虫体的体柄与寄主皮肤接触的部位,可见明显损伤。

语种： 中文

作者： Li, Ming;Li, Weidong;Ge, Xianping*;Wang, Chong;Zhang, Lin;...

期刊： ZOOTAXA,2012年3556(3556):80-88 ISSN：1175-5326

通讯作者： Ge, Xianping

作者机构： [Li, Ming; Huang, Feng; Li, Weidong; Liu, Huiyu] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Ge, Xianping] Chinese Acad Fishery Sci, Minist Agr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China.;[Wang, Chong] Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.;[Wang, Chong] Chinese Acad Sci, Wuhan 430079, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China.

通讯机构： [Ge, Xianping] C;Chinese Acad Fishery Sci, Minist Agr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China.

关键词： Epistylis unioi;peritrich;infraciliature;skin lesions

摘要： The peritrich Epistylis unioi Gong, 1986 was collected from fry of Pelteobagrus fulvidraco during parasite surveys at Honghu Lake Fish Hatchery, Hubei Province, central China in May 2010 and redescribed. Some revisions were done based on live, silver-impregnated, and SEM specimens. The zooid is elongated and somewhat vase-like in shape, measuring 56-88 × 22-38 μm in vivo. A single contractile vacuole is apically located slightly below the peristome disc. The macronucleus is horseshoe-shaped, always transversely situated at the foreside of the body. Haplokinety (H) and polykinety (Po) complete one and one-half circuits on the peristome before entering the infundibulum, with a distal kinetal fragment present at the distal end. Silverline system consists of 37-45 pellicular striations between peristome and aboral trochal band (TB), and of 26-33 between TB and scopula. Colony is asymmetrically and dichotomously branched, usually with only two levels of branches. In addition, the telotrochs of E. unioi were also observed and its structures were described herein. Besides, obvious skin lesions caused by the ringlike base of E. unioi were detected and the relationship between these epizooites and their hosts was briefly discussed as well. Copyright © 2012 · Magnolia Press.

语种： 英文