摘要：
Terpene trilactones (TTL) is a pharmacological ingredient in Ginkgo biloba and its content has become one of the key indices for medicinal value evaluation of ginkgo. 1-Deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) catalyzes the first step specific for isopentenyl diphosphate production in methylerythritol phosphate pathway, which provide the basic structure required for TTLs biosynthesis. To understand the mechanism controlling the GbDXR gene expression, the GbDXR promoter sequence was isolated and subjected to transient expression with the green fluorescent protein (GFP) in tobacco plants. Characteristic analysis revealed various cis-acting elements that related to light-regulated transcription, hormone signaling (auxin, ethylene), adversity stress and defense signaling (heat/dehydration stress) in the GbDXRpromoter region. In transient expression assay, deletion of different portions of the upstream GbDXR promoter identified that the promoter region -3230bp to-865bp conserve the positive regulation function, which could promote the expression of GFP in the cytoplasm of tobacco leaf epidermal cells. The regulation function of the promoter region-865bp to-262bp remained to be elucidated. EMSA analysis suggested possible interactions of GbERF10 and GbERF17 with the ERF-binding elements in the upstream of GbDXR promoter. For abiotic stresses treatment, the expression of GbDXR gene could be significantly induced by UV B and drought stress. In general, the GbDXR gene expressed differently in different ginkgo tissues but exhibited the highest transcriptional level in the root, with the maximum TTLs content simultaneously. The positive relationship between gene expression level and TTLs content indicated that the GbDXR is responsible for TTLs biosynthesis in G. biloba.
摘要：
Obesity develops from an imbalance of energy homeostasis and is associated with the development of metabolic disorders, including insulin resistance and type 2 diabetes. Identification of the underlying molecular mechanisms and effective therapeutic approaches is highly needed. Lysine-specific demethylase 1 (LSD1), an flavin adenine dinucletide-dependent amine oxidase, is implicated in a wide variety of biological processes, including tumorigenesis, stem cell fate decisions, and embryonic development. Recent studies have suggested a vital role of LSD1 in regulating adaptive thermogenesis, mitochondrial biogenesis, glucose, and lipid metabolism. More recently, LSD1 activity was found to be regulated by nutrients, energy status, and hormonal signals, suggesting that it may act as a novel sensor for nutritional regulation of metabolic health. Here, we first discuss the effects of LSD1 on physiological phenotypes, including body weight, fat mass, body temperature, and glucose homeostasis. We also summarize recent understanding of the physiological roles and underlying mechanisms of LSD1 in controlling metabolic functions of adipose and other tissues. Hopefully, a better understanding of the roles of LSD1 in metabolic regulation may provide new perspectives for the nutritional prevention and treatment of obesity.
作者机构：
[Cheng, Shuiyuan; He, Yi; Dong, Xingxing; Wang, Zhangqian; Gao, Chao; Li, Mengjun; Wang, Yidan; Zhang, Sishang; Deng, Jieya; Duan, Yuhua] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan, Peoples R China.;[Wang, Zhangqian; Wang, Qin] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China.;[Yi, Tian] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Peoples R China.
通讯机构：
[Chao Gao; Zhangqian Wang] A;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Selenium is an essential microelement required for human health. The biotransformation of selenium nanoparticles has attracted increasing attention in recent years. However, little of the literature has investigated the comprehensive evaluation of the strains for practical application and the effect on the functional properties in the existence of Se. The present study showed the selenite reduction strain Bacillus subtilis T5 (up to 200 mM), which could produce high yields of selenium polysaccharides and selenium nanoparticles in an economical and feasible manner. Biosynthesized selenium nanoparticles by B. subtilis T5 were characterized systematically using UV-vis spectroscopy, FTIR, Zeta Potential, DLS, and SEM techniques. The biosynthesized SeNPs exhibited high stability with small particle sizes. B. subtilis T5 also possessed a tolerance to acidic pH and bile salts, high aggregation, negative hemolytic, and superior antioxidant activity, which showed excellent probiotic potential and can be recommended as a potential candidate for the selenium biopharmaceuticals industry. Remarkably, B. subtilis T5 showed that the activity of alpha-amylase was enhanced with selenite treatment to 8.12 U/mL, 2.72-fold more than the control. The genus Bacillus was first reported to produce both selenium polysaccharides with extremely high Se-content (2.302 g/kg) and significantly enhance the activity to promote alpha-amylase with selenium treatment. Overall, B. subtilis T5 showed potential as a bio-factory for the biosynthesized SeNPs and organ selenium (selenium polysaccharide), providing an appealing perspective for the biopharmaceutical industry.
通讯机构：
[Yi Wang] S;School of Chemistry and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
insulating gas;trifluoromethanesulfonyl fluoride;Horn's method;acute toxic gas inhalation test;lethal concentration
作者机构：
[Tan, Qiling; Sun, Xuecheng; Huang, Kan; Hu, Chengxiao] Huazhong Agr Univ, Coll Resources & Environm, Microelements Res Ctr, Key Lab Arable Land Conservat Middle & Lower Reach, Wuhan 430070, Peoples R China.;[Tan, Qiling; Sun, Xuecheng; Huang, Kan; Hu, Chengxiao] Huazhong Agr Univ, Coll Resources & Environm, State Environm Protect Key Lab Soil Hlth & Green R, Wuhan 430070, Peoples R China.;[Yu, Min] Foshan Univ, Int Res Ctr Environm Membrane Biol, Foshan 528000, Peoples R China.;[Shabala, Sergey] Univ Tasmania, Tasmanian Inst Agr, Hobart, Tas 7001, Australia.;[Yang, Lin] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Peoples R China.
通讯机构：
[Xuecheng Sun] K;Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Micro-elements Research Center, College of Resources & Environment, Huazhong Agricultural University, Wuhan, 430070, China<&wdkj&>State Environmental Protection Key Laboratory of Soil Health and Green Remediation, College of Resources & Environment, Huazhong Agricultural University, Wuhan, 430070, China
摘要：
Phosphorus-modified biochars are considered as good materials for the removal of heavy metals from wastewater. However, the efficacy of ammonium polyphosphate-modified biochar in cadmium (Cd(II)) adsorption remains largely unknown. In this work, the biochar was respectively modified with ammonium polyphosphate (PABC), phosphoric acid (PHBC) and ammonium dihydrogen phosphate (PNBC) to enhance its adsorption performance for heavy metals from wastewater. The properties of biochar before and after modification and P speciation on the surface of the modified biochar were investigated with FTIR, SEM-EDS, XPS, XRD and (31)P NMR, and the adsorption capacity was evaluated by batch adsorption experiments. The results demonstrated that the optimal adsorption performance could be achieved at the solution pH=4, and the pseudo-second-order and Langmuir models could well describe the Cd(II) adsorption process. The maximum adsorption capacity of PABC, PHBC and PNBC for Cd(II) was 155, 138 and 99mgg(-1), which were 4.84, 4.32 and 3.10 folds that of original biochar, respectively. The (31)P NMR showed that orthophosphate accounted for 82.1%, 62.8% and 54.5% of P in PABC, PHBC and PNBC, respectively, which decreased to 28.24%, 33.51% and 29.34% after Cd(II) adsorption, indicating that the orthophosphate ratio in P-modified biochar surface could significantly affect Cd adsorption by forming phosphate precipitate. This work implies that the PABC has greater potential in the removal of Cd from wastewater relative to PHBC and PNBC.
作者：
Chen, F.;Mei, Q. S.;Li, J. Y.;Li, C. L.;Wan, L.;...
期刊：
Materials Science and Engineering A-structural Materials Properties Microstructure and Processing,2022年839:142859 ISSN：0921-5093
通讯作者：
Mei, QS
作者机构：
[Mei, Q. S.; Xu, T.; Chen, F.; Chen, Z. H.; Li, C. L.; Wang, Y. C.; Mei, X. M.; Tan, Y. Y.; Wan, L.] Wuhan Univ, Sch Power & Mech Engn, Wuhan 430072, Peoples R China.;[Li, J. Y.] Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Mei, QS ] W;Wuhan Univ, Sch Power & Mech Engn, Wuhan 430072, Peoples R China.
关键词：
Cu matrix composites;Dual reinforcements;Synergistic strengthening;Accumulative roll-compositing
摘要：
Cu metal matrix composites (MMCs) incorporated with dual reinforcements of nanocarbons and Al2O3 particles with different contents were prepared by accumulative roll-compositing (ARC). Microstructure and properties of the dual-reinforced Cu MMCs were systematically investigated and compared with those of the individual reinforced counterparts. Results showed that both the individual and dual reinforcements can be homogenously incorporated into and well-bonded with Cu matrix by ARC. The dual-reinforced Cu MMCs exhibited high strength (up to ~747 MPa) and better comprehensive properties of strength/elongation and strength/electrical conductivity than those of the individual counterparts. Moreover, a synergistic strengthening effect of 1 + 1 > 2 of the dual reinforcements was revealed. The influences of content, distribution and interfacial interaction of the dual reinforcements on the properties of the Cu MMCs were discussed. This study indicated the method and mechanisms for further improving the comprehensive properties of Cu MMCs at high strength level by introducing dual reinforcements.
期刊：
Wireless Communications and Mobile Computing,2022年2022 ISSN：1530-8669
通讯作者：
Cui, J.
作者机构：
[Wu, Jike; Cui, Jianqun; Zhang, Ruijie; Chang, Yanan; Wan, Qiyun] Cent China Normal Univ, Sch Comp, Wuhan, Peoples R China.;[Zhou, Hao] Wuhan Polytech Univ, Network & Informatizat Ctr, Wuhan, Peoples R China.
通讯机构：
[Yanan Chang; Qiyun Wan; Jike Wu; Jianqun Cui; Ruijie Zhang] S;[Hao Zhou] N;School of Computer,Central China Normal University,Wuhan,China<&wdkj&>Network and Informatization Center,Wuhan Polytechnic University,Wuhan,China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
How to select proper relay nodes to ensure the successful delivery of messages is still a hot topic in delay tolerant networks (DTN). In this paper, we propose a probabilistic Spray-and-Wait routing algorithm based on node interest preference (called NIP-PSW). Firstly, considering the influence of the social attributes of nodes, we define a metric called node interest preference (NIP) to measure the probability of nodes becoming friends. Secondly, in view of the influence of node quality and connection time between nodes on message forwarding, we define the delivery probability (DP). Finally, according to the historical information of nodes, the node interest similarity (NIS) is proposed. In spray phase, NIP and DP are used to select the relay node and allocate the number of message copies adaptively. In wait phase, it is judged whether to forward the message to the encountering nodes again according to the NIS and the DP. In addition, the concept of message storage value (MSV) and the acknowledgment (ACK) mechanism are introduced to manage the buffer of nodes. The simulation results show that the NIP-PSW not only can significantly improve the delivery rate and reduce the average delay but also shows good performance in network overhead and average number of hops.
通讯机构：
[Qianqian Lin] S;[Qiang Cao] T;The Institute of Technological Sciences, Wuhan University, Wuhan, 430072 China<&wdkj&>School of Physics and Technology, Wuhan University, Wuhan, 430072 China<&wdkj&>Suzhou Institute of Wuhan University, Suzhou, 255123 China
关键词：
femtosecond laser direct writing;micro-grating;perovskite photodetectors;thermal co-evaporation
作者机构：
[Wang, Qian; Zhao, Lin; Wang, Mali; Zhang, Qipeng] Liaocheng Univ, Sch Geog & Environm, Dept Geog Informat Sci, Liaocheng 252059, Shandong, Peoples R China.;[Wu, Jinjia] Liaocheng Univ, Grand Canal Res Ctr, Grand Canal Res Inst, Liaocheng 252000, Shandong, Peoples R China.;[Zhou, Wei] Southwest Univ, Sch Geog Sci, Chongqing Jinfo Mt Karst Ecosyst Natl Observat &, Chongqing 400715, Peoples R China.;[Deng, Meie] Wuhan Polytech Univ, Sch Management, Wuhan 430048, Peoples R China.
通讯机构：
[Qipeng Zhang] D;Department of Geographic Information Science, School of Geography and Environment, Liaocheng University, Liaocheng 252059, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
random forest;grassland drought monitoring;SPEI;drought
摘要：
The accuracy of drought monitoring models is crucial for drought monitoring and early warning. Random forest (RF) is being used widely in the field of artificial intelligence. Nonetheless, the application of a random forest model in grassland drought monitoring research is yet to be further explored. In this study, various drought hazard factors were integrated based on remote sensing data, including from the Moderate Resolution Imaging Spectroradiometer (MODIS) and Global Precipitation Measurement (GPM), as multisource remote sensing data. Based on the RF, a comprehensive grassland drought monitoring model was constructed and tested in Inner Mongolia, China, as an example. The critical issue addressed is the construction of a grassland drought disaster monitoring model based on meteorological data and multisource remote sensing data by using an RF model, and the verification of the accuracy and reliability of its monitoring results. The results show that the grassland drought monitoring model could quantitatively monitor the drought situation in Inner Mongolia grasslands. There was a significantly positive correlation between the drought indicators output by the model and the standardized precipitation evapotranspiration index (SPEI) measured in the field. The correlation coefficients (R) between the drought degree were 0.9706 and 0.6387 for the training set and test set, respectively. The consistent rate between the model drought index and the SPEI reached 87.90%. Drought events in Inner Mongolia were monitored from April to September in wet years, normal years, and dry years using the constructed model. The monitoring results of the model constructed in this study were in accordance with the actual drought conditions, reflecting the development and spatial evolution of drought conditions. This study provides a new application method for the comprehensive assessment of grassland drought.
摘要：
Clustering noisy, high-dimensional, and structurally complex data has always been a challenging task. As most existing clustering methods are not able to deal with both the adverse impact of noisy samples and the complex structures of data, in this paper, we propose a novel Robust and Sparse Possibilistic K-Subspace Clustering algorithm (RSPKS) to integrate subspace recovery and possibilistic clustering algorithms under a unified sparse framework. First, the proposed method sparsifies the membership matrix and the subspace projection vector under a dual-sparse framework to handle high-dimensional noisy data. This unifies dimensionality reduction and clustering using one objective function, for which the optimization can be realized through synchronous iteration. Second, the reconstruction error of each sample in the local subspace is used as the distance metric for classification. That is, each sample itself is treated as a clustering prototype so as not to be affected by the structure of the overall data distribution. Therefore, the clustering prototype construction problem of data with complex structures can be better addressed. Finally, to deal with non-linear regions, our RSPKS method is further extended into a kernelized version, namely the Kernelized Robust and Sparse Possibilistic K-Subspace Clustering (KRSPKS) algorithm. Experimental results on both synthetic and real-world datasets demonstrate that our proposed method outperforms state-of-the-art algorithms in terms of clustering accuracy. IEEE
作者机构：
[Liao, Yangwei; Zhang, Wei; Mu, Yaoqin; Zhang, Lei; Xiao, Xianjin; Hu, Hao; Li, Longjie; Yan, Bei; Ye, Zhengxin] Huazhong Univ Sci & Technol, Inst Reprod Hlth, Tongji Med Coll, Wuhan 430030, People R China.;[Wang, Hongbo; Dong, Kejun; Zhao, Rong; Zhang, Wei; Shu, Wan] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Obstet & Gynecol, Wuhan 430000, People R China.;[Lu, Yaping] Sinopharm Genom Technol Co Ltd, Wuhan 430000, People R China.;[Wan, Chong] Tsinghua Univ, Precis Med Ctr, Yangtze Delta Reg Inst, Jiaxing 314006, Peoples R China.;[Sun, Qiangqiang] Life Hlth Care Clin Labs, Beijing 100000, Peoples R China.
通讯机构：
[Longjie Li; Xianjin Xiao] I;[Hongbo Wang] D;Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, China<&wdkj&>School of Life Science and Technology, Wuhan Polytechnic University , Wuhan 430023, China<&wdkj&>Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430000, China<&wdkj&>Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, China
摘要：
Although CRISPR-Cas12a [clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 12a] combiningpre-amplification technology has the advantage of high sensitivity in biosensing, its generality and specificity are insufficient, which greatly restrains its application range. Here, we discovered a new targeting substrate for LbaCas12a (Lachnospiraceae bacterium Cas12a), namely double-stranded DNA (dsDNA) with a sticky-end region (PAM-SE+ dsDNA). We discovered that CRISPR-Cas12a hadspecial enzymatic properties for this substrate DNA, including the ability to recognize and cleave it without needing a protospacer adjacent motif (PAM) sequence and a high sensitivity to single-base mismatches in that substrate. Further mechanism studies revealed that guide RNA (gRNA) formed a triple-stranded flap structure with the substrate dsDNA. We also discovered the property of low-temperature activation of CRISPR-Cas12a and, by coupling with the unique DNA hybridization kinetics at low temperature, we constructed a complete workflow for low-abundance point mutation detection in real samples, which was fast, convenient and free of single-stranded DNA (ssDNA) transformation. The detection limits were 0.005-0.01% for synthesized strands and 0.01-0.05% for plasmid genomic DNA, and the mutation abundances provided by our system for 28 clinical samples were in accordance with next-generation sequencing results. We believe that our work not only reveals novel information about the target recognition mechanism of the CRISPR-Cas12a system, but also greatly broadens its application scenarios.
通讯机构：
[Weiwei Zhang; Feng Xu] C;College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China<&wdkj&>College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China<&wdkj&>Spice Crops Research Institute, Yangtze University, Jingzhou 434025, Hubei, China
