摘要：
Pseudonocardia species are emerging as important microorganisms of global concern with unique and increasingly significant ecological roles and represent a prominent source of bioactive natural products, but genetic engineering of these organisms for biotechnological applications is greatly hindered due to the limitation of efficient genetic manipulation tools. In this regard, we report here the establishment of an efficient genetic manipulation system for a newly isolated strain, Pseudonocardia alni Shahu, based on plasmid conjugal transfer from Escherichia coli to Pseudonocardia. Conjugants were yielded upon determining the optimal ratio between the donor and recipient cells, and designed genome modifications were efficiently accomplished, including exogenous gene integration based on an integrative plasmid and chromosomal stretch removal by homologous recombination using a suicidal non-replicating vector. Collectively, this work has made the P. alni Shahu accessible for genetic engineering, and provided an important reference for developing genetic manipulation methods in other rare actinomycetes.
作者机构：
[Liu, Siyuan; Li, Longquan; Sui, Zhiwei; Wang, Meng; Wang, Ziquan] Natl Inst Metrol, Ctr Adv Measurement Sci, 18 Beisanhuandonglu, Beijing 100029, Peoples R China.;[Wang, Zejian; Li, Bo] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China.;[Li, Longquan; Zhou, Guoping] Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan, Peoples R China.
通讯机构：
[Zejian Wang] S;[Zhiwei Sui] C;Center for Advanced Measurement Science, National Institute of Metrology, Beijing, China<&wdkj&>State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
作者：
Su, Liangxia;Hua, Dong;Liu, Jun;Hu, Bing;Wang, Jianwei
期刊：
Water,2022年14(13):2084- ISSN：2073-4441
通讯作者：
Jun Liu
作者机构：
[Su, Liangxia; Hua, Dong; Liu, Jun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.;[Hu, Bing] Fujian Key Lab Special Aquat Formula Feed, Fuzhou 350308, Peoples R China.;[Wang, Jianwei] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
通讯机构：
[Jun Liu] S;School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
The wide use of rare-earth elements in China for aquacultural purposes and many other applications has resulted in their accumulation in the aquatic environment and has caused concern about their safety. In this study, we tested the toxicity of lanthanum (La (III)) to the early life stages (embryonic and sac-fry stages) of the rare minnow Gobiocypris rarus Ye & Fu, 1983. We exposed fertilized eggs to 0, 0.06, 0.13, 0.25, 0.50, 1.00, and 1.92 mg/L of La (III) until the yolk sac was exhausted in any group (at about 168 h of exposure). Exposure to 1.00 and 1.92 mg/L La (III) had obvious lethal effects on embryos, La (III) exposure also accelerated the development of embryos and had a significant inhibitory effect on the hatching rate after 96 h. As the exposure time increased, the larvae exhibited obvious yolk-sac edema, pericardium edema, spinal curvature, tail bending, and other symptoms of poisoning, including deflated swim-bladder. In general, these results clearly indicate that La pollutants hinder the development of rare minnow embryos and are acutely toxic to rare minnow larvae. Our finding would provide a theoretical basis for further research of relevant feed additive criteria for this fish.
通讯机构：
[Xuesong Yu] S;School of Economics and Management, Hubei Polytechnic University, Huangshi 435003, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
mask;green supply chain;anti-risk;pandemic
摘要：
Against the background of the pandemic, the mask supply chain faces the risk of pollution caused by discarded masks, the risk of insufficient funds of retailers, and the risk of mask overstock. To better guard against the above risks, this study constructed a two-party game model and a cusp catastrophe model from the perspective of the mask green supply chain, and studied the strategic choices of retailers and suppliers in the supply chain affected by the risk of capital constraints and overstock. The result shows that the risk shocks will lead to the disruption of the mask green supply chain, and the main factors affecting the strategy choice of mask suppliers and retailers are mask recycling rate, deposit ratio, risk occurrence time, etc. In further research, this study involved a mechanism for financial institutions, mask retailers, and the government to jointly deal with the risk of mask overstock, the risk of retailers’ insufficient funds, and the risk of environmental pollution from discarded masks. The research path and conclusion of this study reveal the risks in the circulation area of mask supplies during the pandemic, and provide recommendations for planning for future crises and risk prevention.
期刊：
International Journal of Environmental Research and Public Health,2022年19(13):7990- ISSN：1661-7827
通讯作者：
Chong Zhao
作者机构：
[Xiao, Pengnan] Cent China Normal Univ, Coll Urban & Environm Sci, Wuhan 430079, Peoples R China.;[Xu, Jie] Hubei Univ, Fac Resources & Environm Sci, Wuhan 430062, Peoples R China.;[Zhao, Chong] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430040, Peoples R China.
通讯机构：
[Chong Zhao] S;School of Chemistry and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430040, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
“production-living-ecological” space;space conflict;FLUS model;land use change
作者机构：
[Liu, Mengyi; Lu, Haijun; Ji, Shuang; Deng, Qingkai] Wuhan Polytech Univ, Sch Civil Engn & Architecture, Wuhan 430023, Peoples R China.;[Liu, Mengyi; Lu, Haijun; Wan, Yong] Chinese Acad Sci, Inst Rock & Soil Mech, State Key Lab Geomech & Geotech Engn, Wuhan 430071, Peoples R China.;[Qin, Linbo] Wuhan Univ Sci & Technol, Coll Resources & Environm Engn, Wuhan 430081, Peoples R China.
通讯机构：
[Haijun Lu; Yong Wan] S;School of Civil Engineering and Architecture, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>State Key Laboratory of Geomechanics and Geotechnical Engineering, Institute of Rock and Soil Mechanics, Chinese Academy of Sciences, Wuhan 430071, China<&wdkj&>State Key Laboratory of Geomechanics and Geotechnical Engineering, Institute of Rock and Soil Mechanics, Chinese Academy of Sciences, Wuhan 430071, China
关键词：
Industrial solid waste;Landfill cover;Microstructure;Modified municipal sludge;Water permeability
摘要：
In order to prepare a new type of landfill covering material for closure, we used industrial calcium-containing waste (slag, desulfurised gypsum and fly ash) to modify the municipal dewatered sludge. Shear, infiltration and rainfall infiltration model tests were performed to obtain the shear strength parameters of the modified sludge, the hydraulic conductivity during the wet-dry cycle, and the service performance against rainfall breakdown to evaluate the service performance of the modified sludge cover (MSC). Comprehensive characterisation of the modified sludge was analysed by XRD, FTIR and SEM-EDS to revealed the mineral structure, microstructure, and modification mechanism of the sludge. The MSC samples had high shear strength and shown the characteristics of evolving from plasticity to brittleness. After curing for 14 d, the values of cohesion c and internal friction angle φ reached 150.75-384.69kPa and 37.60-57.29°, respectively. The MSC exhibited excellent anti-seepage service performance under dry and wet cycle conditions. Compared with traditional compacted clay, its hydraulic conductivity dropped by an order of magnitude, and after six wet and dry cycles, the hydraulic conductivity of the modified sludge reached stability at 1.4-7.2×10(-7) cm/s. The 60-cm-thick MSC layer can completely withstand the impact of long-term rainfall during the rainy season in the middle reaches of the Yangtze River in China. Analysis results also show that the modification mechanism of the sludge could be ascribed to the generation of dense blocks and clusters of C-S-H and C-A-S-H gels originated from SiO(2), Al(2)O(3), and CaO phases in industrial calcium-containing waste and sludge by the activation of the alkali.
摘要：
Cardiovascular complications of patients with type 2 diabetes mellitus (T2DM) threaten the health and life of numerous individuals. Recently, growth factor receptor-binding protein 10 (GRB10) was found to play a pivotal role in vascular complications of T2DM, which participates in the regulation of lipid metabolism of T2DM patients. The genetic variation of GRB10 rs1800504 is closely related to the risk of coronary heart disease in patients with T2DM. The development of GRB10 as a key mediator in the association of lipid metabolism with cardiovascular complications in T2DM is detailed in and may provide new potential concerns for the study of cardiovascular complications in T2DM patients.
作者机构：
[Guo Yuhao; Cao Yan; Wang Feifei] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Wang Feifei] Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China.;[Wang Feifei; Zhang Chengwu] Minist Educ, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn, Guangzhou 510632, Peoples R China.;[Zhang Chengwu] Jinan Univ, Dept Ecol, Guangzhou 510632, Peoples R China.;[Zhang Chengwu] Jinan Univ, Res Ctr Hydrobiol, Guangzhou 510632, Peoples R China.
通讯机构：
[Chengwu Zhang] E;Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou, China<&wdkj&>Department of Ecology and Research Center for Hydrobiology, Jinan University, Guangzhou, China
摘要：
Filamentous microalgae from genus Tribonema are promising sustainable sources of omega-7 palmitoleic acid, but their ability to accumulate this compound varies among species and depends on the initial nitrogen concentration (INC) supply. In this study, the palmitoleic acid accumulation capacities of five Tribonema species were examined under three different INCs to select the alga species with the highest production. Results showed that a high INC was associated with increased palmitoleic acid accumulation but led to decreased biomass concentration in all tested species. In particular, T. minus grown at 18 mmol L~(-1) INC had the highest palmitoleic acid content (20.72% of dry weight) and productivity (90.88 mg L~(-1) d~(-1)). The combination of alkali metal freezing precipitation (AMFP) and urea complexation successfully isolated and enriched palmitoleic acid from T. minus and obtained a purity of 80.11% and a yield of 7.39 g (100 g)-1 of algal powder. The compound was identified as (9Z)-hexadecenoic acid (C16:1 ω-7). Antibacterial activity evaluation for the highly concentrated palmitoleic acid (10 mg mL~(-1)) against Streptococcus agalactiae revealed the formation of a 12.10 mm-diameter inhibition zone and the minimum inhibitory concentration of 31.25 μg mL~(-1), indicating that palmitoleic acid is an effective antibacterial agent. This study is the first to report that palmitoleic acid derived from T. minus can antagonize S. agalactiae, which further broadens the potential application of Tribonema biomass in green aquaculture.
摘要：
In this paper, the properties and evolution during the calcination process of hydrolysis by−products from Al‐water reaction for hydrogen generation were systematically investigated. The phase composition of by−products included AlOOH, InSn4, and trace phases. AlOOH constructed with flower‐like shape with micro/nano petals was obtained from the hydrolysis reaction. The phase transformation from AlOOH to γ−Al2O3, γ− Al2O3, and α−Al2O3 occurs during the calcination process. Discussion on the properties and evolution of hydrolysis by−products are beneficial to product reuse and recovery. Abstract In this paper, the properties and evolution during the calcination process of hydrolysis by−products from the reaction between Al−based composites and water for hydrogen generation were systematically investigated. The results showed that the main phases of by−products included AlOOH and InSn4. The amorphous product possessed a large Brunauer−Emmett−Teller (BET) surface area with 276.77 m2/g and high porosity properties with 0.37 cm3/g. The phase transformation of by−products from AlOOH to γ−Al2O3, δ−Al2O3, and α−Al2O3 occurred during the calcination process. It appears that the by−products can be used as raw materials for the preparation of Al2O3 with different crystal structures. Discussion on the properties and evolution of hydrolysis by−products are beneficial to product reuse and recovery. In this way, the cost reduction of hydrogen production via Al−water reaction can be realized, promoting the sustainable development of hydrogen production from Al−water reaction.
作者机构：
[Wang, Zemin; An, Jiachun; Ma, Yuanyuan; Li, Fei; Liu, Shunlun; Li, Bing] Wuhan Univ, Chinese Antarctic Ctr Surveying & Mapping, Wuhan 430079, Peoples R China.;[Ma, Yuanyuan; Li, Fei] Wuhan Univ, Elect Informat Sch, Wuhan 430072, Peoples R China.;[Wang, Zemin; An, Jiachun; Ma, Yuanyuan; Li, Fei; Liu, Shunlun] MNR, Key Lab Polar Surveying & Mapping, Wuhan 430079, Peoples R China.;[Li, Bing] Wuhan Polytech Univ, Sch Math & Comp Sci, Wuhan 430048, Peoples R China.;[Ma, Weifeng] Yunnan Normal Univ, Fac Geog, Kunming 650050, Yunnan, Peoples R China.
通讯机构：
[Wang, ZM ] W;Wuhan Univ, Chinese Antarctic Ctr Surveying & Mapping, Wuhan 430079, Peoples R China.;MNR, Key Lab Polar Surveying & Mapping, Wuhan 430079, Peoples R China.
关键词：
glacier mass balance;SAR interferometry;ice motion;ionospheric effects;reformulation of the split-spectrum method;ionospheric correction
摘要：
Ice motion is an essential element for accurately evaluating glacier mass balance. Interferometric synthetic aperture radar (InSAR) has been widely applied for monitoring ice motion with high precision and wide coverage in the Antarctic. However, the ionospheric effects can significantly impact InSAR-based ice-motion measurements. At low radar frequencies in particular, the ionospheric effects have been regarded as a serious source of noise in L-band SAR data. The split-spectrum method (SSM) is commonly used for correcting the ionospheric effects of the InSAR technique. However, it requires spatial filtering with the relatively large factors used to scale the sub-bands' interferograms, which often results in an unwrapped phase error. In this paper, a reformulation of the split-spectrum method (RSSM) is introduced to correct the ionospheric effects in the Grove Mountains of East Antarctica, which have slow ice flow and frequent ionosphere changes. The results show that RSSM can effectively correct the ionospheric effects of InSAR-based ice-motion measurements. To evaluate the ability of ionospheric correction using RSSM, the result of ionospheric correction derived from SSM is compared with the results of RSSM. In addition, ionosphere-corrected ice motion is also compared with GPS and MEaSUREs. The results show that the ionosphere-corrected ice velocities are in good agreement with GPS observations and MEaSUREs. The average ice velocity from the InSAR time series is compared to that from MEaSUREs, and the average ionosphere-corrected ice velocity error reduces 43.9% in SSM and 51.1% in RSSM, respectively. The ionosphere-corrected ice velocity error is the most significant, reducing 86.9% in SSM and 90.4% in RSSM from 1 November 2007 to 19 December 2007. The results show that the ability of RSSM to correct ionospheric effects is slightly better than that of SSM. Therefore, we deduce that the RSSM offers a feasible way to correct ionospheric effects in InSAR-based ice-motion measurements in Antarctica.
通讯机构：
[Bingying Ding; Shuangshuang Guo] A;Authors to whom correspondence should be addressed.<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-Products, Wuhan Polytechnic University, Ministry of Education, Wuhan 430023, China
摘要：
Coccidia and C. perfringens are the main accomplices in broiler necrotizing enteritis (NE), and NE causes oxidative stress and intestinal damage in broilers, which will result in huge economic losses to the poultry industry. Vitamin A (VA) is an essential micronutrient of the diet, and the beneficial effects of VA on vision, growth, and development have been intensively investigated. At present, the effects of VA on intestinal barrier and antioxidant functions in broilers have not been systematically reported. The present study was conducted to investigate the effects of dietary supplementation with vitamin A on the antioxidant and intestinal barrier function of broilers co-infected with coccidia and C. perfringens (CCP). Our outcomes showed that dietary VA might help a little with intestinal barrier function; nonetheless, it failed to alleviate the negative effects of CCP on the antioxidant function in broilers. Our study has guiding significance for the dose of VA in the diet of broilers, and it might arouse readers' strong interest. Necrotic enteritis (NE) impairs poultry production and causes great economic loss. The nutritional regulation of diets has the potential to alleviate NE. The present study was conducted to investigate the effects of dietary supplementation with vitamin A (VA) on the antioxidant and intestinal barrier function of broilers co-infected with coccidia and C. perfringens (CCP). In a 2 x 2 factorial arrangement, 336 one-day-old Ross 308 broilers were divided into four treatments with two levels of VA (0 or 12,000 IU/kg) and challenged with or without CCP. The animal trial lasted for 42 days. The results showed that dietary supplemental VA improved body weight gain (BWG) and the feed intake (FI), and the FI was negatively affected by CCP. Additionally, the levels of catalase (CAT) in the serum, total superoxide dismutase (T-SOD), and CAT in the jejunum and glutathione peroxidase (GSH-Px) in the liver decreased with the CCP challenge (p < 0.05). The mRNA levels of SOD, CAT, GSH-Px1, and GSH-Px3 in the liver and jejunum were upregulated by the CCP challenge (p < 0.05). In addition, the level of serum diamine oxidase (DAO), and the mRNA level of ZO-1 were also upregulated with the CCP challenge. Dietary supplementation with VA contributed to the intestinal villi height and the mRNA level of Mucin-2 in the jejunum (p < 0.05). Additionally, dietary VA had the ability to alleviate the upregulation of SOD in the liver and SOD, CAT, GSH-Px1, GSH-Px3, ZO-1, and claudin-1 in the jejunum with the CCP challenge (p < 0.05). However, the mRNA level of GSH-Px3 and the levels of SOD in the liver and jejunum were downregulated with the VA supplementation in the diet. In conclusion, dietary VA improved the growth performance and the intestinal barrier function; nonetheless, it failed to alleviate the negative effects of CCP on the antioxidant function in broilers.
通讯机构：
[Kaiwu zhang; Wei Wang] G;[Dengxiang Du] S;Guizhou Institute of Upland Food Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China<&wdkj&>School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
Embryogenic and regenerable tissue cultures are widely used in plant transformation. To dissect the molecular mechanism of embryogenesis, we used inbred line A188 as the material; the immature embryo of kernels (15 day after pollination, 15DAP) was isolated and cultured in inducing medium and subjected to RNA-Seq. The results revealed that 5,076 differentially expressed genes (DEGs) were involved in morphological and histological changes and endogenous indole-3-acetic acid (IAA) alteration. Functional analysis showed that the DEGs were related to metabolic pathways and biosynthesis of secondary metabolites. In particular, ARF16 and ARF8 genes of auxin response factors (ARF) were upregulated from EC to IDC and EC to IRC. Meanwhile, BBM2, SERK1, and SERK2 genes of the embryogenic pathway were upregulated, and WIP2 and ESR genes of the wound-inducible were upregulated from EC to IDC and EC to IRC. These changes can improve conversion efficiency from EC to IRC, which is important for elucidating the underlying molecular mechanisms of callus formation.
