摘要：
Obesity develops from an imbalance of energy homeostasis and is associated with the development of metabolic disorders, including insulin resistance and type 2 diabetes. Identification of the underlying molecular mechanisms and effective therapeutic approaches is highly needed. Lysine-specific demethylase 1 (LSD1), an flavin adenine dinucletide-dependent amine oxidase, is implicated in a wide variety of biological processes, including tumorigenesis, stem cell fate decisions, and embryonic development. Recent studies have suggested a vital role of LSD1 in regulating adaptive thermogenesis, mitochondrial biogenesis, glucose, and lipid metabolism. More recently, LSD1 activity was found to be regulated by nutrients, energy status, and hormonal signals, suggesting that it may act as a novel sensor for nutritional regulation of metabolic health. Here, we first discuss the effects of LSD1 on physiological phenotypes, including body weight, fat mass, body temperature, and glucose homeostasis. We also summarize recent understanding of the physiological roles and underlying mechanisms of LSD1 in controlling metabolic functions of adipose and other tissues. Hopefully, a better understanding of the roles of LSD1 in metabolic regulation may provide new perspectives for the nutritional prevention and treatment of obesity.
作者机构：
[Chao, Wei; Chen, Qiangwen; Ye, Jiabao; Xu, Feng; Zhang, Weiwei; Liao, Yongling] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Peoples R China.;[Chao, Wei] Yangtze Univ, Minist Educ, Engn Res Ctr Ecol & Agr Use Wetland, Jingzhou 434025, Peoples R China.;[Chao, Wei] Yangtze Univ, Hubei Key Lab Waterlogging Disaster & Agr Use Wet, Jingzhou 434025, Peoples R China.;[Cheng, Shuiyuan; Rao, Shen] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.;[Yang, Xiaoyan] Shaoguan Univ, Henry Fok Sch Biol & Agr, Shaoguan 512005, Peoples R China.
通讯机构：
[Xiaoyan Yang; Feng Xu] A;Authors to whom correspondence should be addressed.<&wdkj&>Henry Fok School of Biology and Agricultural, Shaoguan University, Shaoguan 512005, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
关键词：
selenium;plant;allelopathy;ecosystem
摘要：
Selenium is an essential trace element which plays an important role in human immune regulation and disease prevention. Plants absorb inorganic selenium (selenite or selenate) from the soil and convert it into various organic selenides (such as seleno amino acids, selenoproteins, and volatile selenides) via the sulfur metabolic pathway. These organic selenides are important sources of dietary selenium supplementation for humans. Organoselenides can promote plant growth, improve nutritional quality, and play an important regulatory function in plant ecosystems. The release of selenium-containing compounds into the soil by Se hyperaccumulators can promote the growth of Se accumulators but inhibit the growth and distribution of non-Se accumulators. Volatile selenides with specific odors have a deterrent effect on herbivores, reducing their feeding on plants. Soil microorganisms can effectively promote the uptake and transformation of selenium in plants, and organic selenides in plants can improve the tolerance of plants to pathogenic bacteria. Although selenium is not an essential trace element for plants, the right amount of selenium has important physiological and ecological benefits for them. This review summarizes recent research related to the functions of selenium in plant ecosystems to provide a deeper understanding of the significance of this element in plant physiology and ecosystems and to serve as a theoretical basis and technical support for the full exploitation and rational application of the ecological functions of selenium-accumulating plants.
作者机构：
[Chen, Qiangwen; Xu, Feng; Xiang, Juan; Zhang, Weiwei] Yangtze Univ, Coll Hort & Gardening, Jinzhou 434025, Peoples R China.;[Cheng, Shuiyuan; Rao, Shen] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Zhang, Yue; Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.;[Yang, Xiaoyan] Shaoguan Univ, Henry Fok Sch Biol & Agr, Shaoguan 512005, Peoples R China.
通讯机构：
[Xiaoyan Yang; Feng Xu] A;Authors to whom correspondence should be addressed.<&wdkj&>Henry Fok School of Biology and Agricultural, Shaoguan University, Shaoguan 512005, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Horticulture and Gardening, Yangtze University, Jinzhou 434025, China
关键词：
selenium;Camellia sinensis (L.) O. Kuntze;growth;quality
摘要：
Selenium (Se) is an essential trace element for humans and animals, and it plays an important role in immune regulation and disease prevention. Tea is one of the top three beverages in the world, and it contains active ingredients such as polyphenols, theanine, flavonoids, and volatile substances, which have important health benefits. The tea tree has suitable Se aggregation ability, which can absorb inorganic Se and transform it into safe and effective organic Se through absorption by the human body, thereby improving human immunity and preventing the occurrence of many diseases. Recent studies have proven that 50 similar to 100.0 mg/L exogenous Se can promote photosynthesis and absorption of mineral elements in tea trees and increase their biomass. The content of total Se and organic selenides in tea leaves significantly increases and promotes the accumulation of polyphenols, theanine, flavonoids, and volatile secondary metabolites, thereby improving the nutritional quality of tea leaves. This paper summarizes previous research on the effects of exogenous Se treatment on the growth and quality of tea trees to provide a theoretical basis and technical support for the germplasm selection and exploitation of Se-rich tea.
作者机构：
[Jia, Jilai; He, Jiangling; Zhou, Jiaojiao; Cai, Jie; Lv, Xuqin; Cai, Shiqi; Xie, Fang] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Lv, Xuqin] Wuhan Polytech Univ, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;[Din, Zia-ud] Univ Swabi, Dept Agr, Swabi 23561, Pakistan.
通讯机构：
[Jie Cai] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Developing robust and sensitive food safety detection methods is important for human health. Electrochemiluminescence (ECL) is a powerful analytical technique for complete separation of input source (electricity) and output signal (light), thereby significantly reducing background ECL signal. ECL biosensors have attracted considerable attention owing to their high sensitivity and wide dynamic range in food safety detection. In this review, we introduce the principles of ECL biosensors and common ECL luminophores, as well as the latest applications of ECL biosensors in food analysis. Further, novel nanomaterial assembly strategies have been progressively incorporated into the design of ECL biosensors, and by demonstrating some representative works, we summarize the development status of ECL biosensors in detection of mycotoxins, heavy metal ions, antibiotics, pesticide residues, foodborne pathogens, and other illegal additives. Finally, the current challenges faced by ECL biosensors are outlined and the future directions for advancing ECL research are presented.
通讯机构：
[Yulan Liu] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Simple Summary Oxidative stress may reduce the growth performance and intestinal health status of weanling piglets. Due to the fact that the body can synthesize glycine, it is generally treated as an amino acid which is nonessential for nourishment. However, previous research has demonstrated that synthesized glycine was unable to support piglets' newborn growth and development. Moreover, according to several findings, glycine is crucial for relieving oxidative stress and intestinal damage. The purpose of this investigation was to determine whether glycine could lessen the intestinal damage caused by diquat in weanling piglets and the relationship between ferroptosis and diquat-induced intestinal epithelial cell death. The results showed that dietary glycine reduced intestinal oxidative stress induced by diquat in weanling piglets. Furthermore, with increasing anti-oxidative capacity, dietary glycine was able to restrain intestinal epithelial cell ferroptosis triggered by diquat. The purpose of this research was to examine the impact of glycine on intestinal injury caused by oxidative stress in piglets. A 2 x 2 factorial experiment with diets (basic diet vs. 1% glycine diet) and oxidative stress (saline vs. diquat) was conducted on 32 weanling piglets. On day 21, all piglets received an injection of either saline or diquat. After 7 days, all pigs were slaughtered and intestinal samples were collected. Dietary glycine supplementation improved intestinal mucosal morphology, increased the activities of disaccharidases and enhanced intestinal mucosal antioxidant capacity, while regulating the expression of ferroptosis mediators in the piglets under oxidative stress. These findings suggested that dietary glycine supplementation improved the morphology and function of the intestinal mucosa, which was involved in regulating antioxidant capacity and ferroptosis.
通讯机构：
[Yulan Liu] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Lay Summary Aflatoxin B1 (AFB1) is well known for its growth retardation, hepatotoxic, immunosuppressive, and other negative effects both in humans and poultry. Plant extracts such as tannic acid (TA) have been demonstrated as effective agents to control AFB1 contamination. The objective of this study was to evaluate the effects of Chinese gallnut TA in preventing aflatoxicosis in broilers. Broilers received one of four treatments: CON, control diet; AF, control diet with AFB1; TA1, AF + 250 mg/kg TA; TA2, AF + 500 mg/kg TA. Although AF did not decrease the growth performance of broilers, 250 and 500 mg/kg TA had greater average daily gain and average daily feed intake than those in the CON and AF. The relative weight of liver and kidney, blood alanine transferase, and gamma-glutamyl transferase activity were increased, and the antioxidant status was depressed in chicks fed the AF diet compared with the CON group. Dietary supplementation with 250 and 500 mg/kg TA ameliorated all the above-mentioned negative effects of AFB1. Moreover, the crypt depth of the jejunum was decreased, and the villus height of the ileum was increased in TA treatments compared with the AF. Conclusively, Chinese gallnut TA could be considered as a potential natural agent for the prevention of AFB1-induced oxidative and intestinal damage of broilers. The objective of the present study was to investigate the effects of tannic acid (TA) on growth performance, blood parameters, antioxidant capacity, and intestinal health in broilers challenged with aflatoxin B1 (AFB1). A total of 480 broilers aged 1 d were randomly allotted into four treatments: 1) CON, control diet; 2) AF, CON + 60 mu g/kg AFB1 of feed during days 1 to 21, CON + 120 mu g/kg AFB1 of feed during days 22 to 42; 3) TA1, AF + 250 mg/kg TA; and 4) TA2, AF + 500 mg/kg TA. Average daily gain (ADG) and average daily feed intake (ADFI) were increased in the TA1 during days 1 to 21, days 22 to 42, and days 1 to 42 compared with CON and AF treatments (P < 0.05). Broilers fed the TA2 diet had greater ADG and ADFI than those fed the CON and AF diets during the finisher and the whole period (P < 0.05). Administration of TA decreased the relative weight of liver and kidney compared with broilers fed the AF diet on day 42 (P < 0.05). The blood activity of alanine transferase (ALT) and gamma-glutamyl transferase (GGT) was increased in the AF treatment compared with the CON (P < 0.05). Broilers fed the TA1 decreased the ALT content on day 21, and the level of ALT and GGT was decreased in the TA2 compared with the AF group on day 42 (P < 0.05). The activity of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) in plasma, and the hepatic glutathione S-transferase (GST) was decreased in the AF group compared with the CON group (P < 0.05). The TA decreased plasma malondialdehyde concentration, and increased plasma T-SOD, GSH-Px, total antioxidant capacity, and hepatic GST activity compared with the AF (P < 0.05). The crypt depth of the jejunum was decreased in the TA1 treatment on day 21, and the villus height of the ileum was increased in the TA2 group on day 42 compared with the AF treatment (P < 0.05). The cecal Lactobacillus counts on day 21 were tended to increase in the TA treatments compared with the AF (P = 0.061). In conclusion, dietary inclusion of 250 and 500 mg/kg TA could improve the growth, antioxidant capacity, and partially protected the intestinal health of broilers challenged with AFB1. Dietary supplementation with 250 and 500 mg/kg Chinese gallnut tannins could provide protection against the negative effects of aflatoxin B1 on the liver, antioxidative status, and intestinal morphology in broiler chickens.
作者机构：
[An, Xiaoyu; Guo, Ziqi; Zhang, Rui; Wu, Muci; Tao, Wen; He, Jingren] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pr, Sch Modern Ind Selenium Sci & Engn, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.;[Zhang, Rui; He, Jingren; Yang, Ning] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.;[Oliveira, Helder] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE, Rua Campo Alegre S-N, P-4169007 Porto, Portugal.
通讯机构：
[Rui Zhang; Jingren He] N;National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, No. 36 Huanhu Middle Road, Jinyinhu District, Wuhan 430023, P. R. China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, No. 36 Huanhu Middle Road, Jinyinhu District, Wuhan 430023, P. R. China
作者机构：
[Li, Yu; Cui, Haoran; Wang, Zhipeng; Song, Jian] North Univ China, Sch Chem Engn & Technol, Taiyuan 030051, Peoples R China.;[Mu, Jiliang; Zhang, Le; Cui, Haoran; Chou, Xiujian] North Univ China, Sch Instrument & Elect, Taiyuan 030051, Peoples R China.;[Cao, Guoqiang] Chinese Acad Sci, Inst Coal Chem, State Key Lab Coal Convers, Taiyuan 030001, Peoples R China.;[Wu, Yiwan] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Zhipeng Wang; Xiujian Chou] A;Authors to whom correspondence should be addressed.<&wdkj&>These authors contributed equally to this work.<&wdkj&>School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>School of Instrument and Electronics, North University of China, Taiyuan 030051, China
摘要：
In the present work, a facile one-step methodology was used to synthesize honeycomb-like BiFeO3/g-C3N4 composites, where the well-dispersed BiFeO3 strongly interacted with the hg-C3N4. The 10BiFeO(3)/hg-C3N4 could completely degrade RhB under visible light illumination within 60 min. The degradation rate constant was remarkably improved and approximately three times and seven times that of pristine hg-C3N4 and BiFeO3, respectively. This is ascribed to the following factors: (1) the unique honeycomb-like morphology facilitates the diffusion of the reactants and effectively improves the utilization of light energy by multiple reflections of light; (2) the charged dye molecules can be tightly bound to the spontaneous polarized BiFeO3 surface to form the Stern layer; (3) the Z-scheme heterojunction and the ferroelectric synergistically promoted the efficient separation and migration of the photogenerated charges. This method can synchronously tune the micro-nano structure, surface property, and internal field construction for g-C3N4-based photocatalysts, exhibiting outstanding potential in environmental purification.
作者机构：
[Feng, Yun] Wuhan Polytech Univ, Sch Math & Comp Sci, Wuhan 430023, Peoples R China.;[Lin, Wensong] Southeast Univ, Sch Math, Nanjing 210096, Peoples R China.
通讯机构：
[Yun Feng] S;School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
期刊：
Critical Reviews in Food Science and Nutrition,2022年:1-15 ISSN：1040-8398
通讯作者：
Krystian Marszałek
作者机构：
[Khaneghah, Amin Mousavi; Chen, Zhe; Marszalek, Krystian] Prof Waclaw Dabrowski Inst Agr & Food Biotechnol, State Res Inst, Dept Fruit & Vegetable Prod Technol, Warsaw, Poland.;[Spilimbergo, Sara] Univ Padua, Dept Ind Engn, Padua, Italy.;[Zhu, Zhenzhou] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan, Peoples R China.
通讯机构：
[Krystian Marszałek] D;Department of Fruit and Vegetable Product Technology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology – State Research Institute, Warsaw, Poland
关键词：
Economic analysis;fruit and vegetable;future challenges;preservation;regulation;supercritical carbon dioxide
摘要：
Consumers have an increasing demand for fruit and vegetables with high nutritional value worldwide. However, most fruit and vegetables are vulnerable to quality loss and spoilage during processing, transportation, and storage. Among the recently introduced emerging technologies, supercritical carbon dioxide (SCCO2) has been extensively utilized to treat and maintain fruit and vegetables mainly due to its nontoxicity, safety, and environmentally friendly. SCCO2 technology generates low processing costs and mild processing conditions (temperature and pressure) that allow for the application of CO2 at a supercritical state. This review aimed to summarize the current knowledge on the influence of SCCO2 technology on the quality attributes of fruit and vegetable products, such as physicochemical properties (pH, color, cloud, particle size distribution, texture), sensory quality, and nutritional composition (ascorbic acid, phenolic compounds, anthocyanins, carotenoids, and betalains). In addition, the effects and mechanisms of the SCCO2 technique on endogenous enzyme inactivation (polyphenol oxidase, peroxidase, and pectin methylesterase) were also elucidated. Finally, the prospects of the SCCO2 technique for industrial application was discussed from the economic and regulatory aspect.
通讯机构：
[Yang, Jiang-Tao] C;[Huo, Kai-Fu] W;[Zhu, Lian] S;Wuhan National Lab for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China<&wdkj&>College of Life Sciences and Health, Wuhan University of Science and Technology, Wuhan 430070, China<&wdkj&>School of Chemistry and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Incomplete tumor ablation and subsequent tumor metastasis usually occur during photothermal anti-tumor processes. The combination of photothermal and immunotherapy has proven to be a promising method to conquer technical challenges. Inhibiting the programmed death ligand-1 (PD-L1)/programmed cell death protein 1 (PD-1) immune pathway represents one of the most successful immunotherapy strategies. Whereas, the PD-L1 expression level significantly differs, leading to a relatively low response rate to the immune checkpoint blockade (ICB) approaches. Therefore, improving the expression level of PD-L1 becomes one potential method to enhance the response rate. Herein, NIH 3T3 cells were educated to steadily express PD-1 protein. Furthermore, the synthesized molybdenum nitride was then coated with PD-1 protein-modified cytomembrane, which endows it with immune checkpoint blocking capability. Moreover, under the irradiation of near-infrared light, the local mild heat released from the molybdenum nitride causes the apoptosis of tumor cells. More importantly, the elevated temperature simultaneously helps elevate the expression level of PD-L1, further enhancing the response rate of ICB. Finally, the PD-1 cytomembrane coatings interact with the upregulated PD-L1, leading to the activation of the immune system. In summary, we confirmed that the PD-1 protein-coated molybdenum nitride could synergistically ablate tumors and avoid metastasis.
通讯机构：
[Tongbo Wu] D;Department of Pharmaceutical Analysis, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
摘要：
Alkaline phosphatase (ALP) is widely expressed in human tissues. ALP plays an important role in the dephosphorylation of proteins and nucleic acids. Therefore, quantitative analysis of ALP plays a vital role in disease diagnosis and the development of biological detection methods. Terminal deoxynucleotidyl transferase (TdT) catalyzes continuous polymerization of deoxynucleotide triphosphates at the 3'-OH end of single-stranded DNA in the absence of a template. In this study, we developed a highly sensitive and selective method based on TdT and endonuclease IV (Endo IV) to quantify ALP activity. After ALP hydrolyzes the 3'-PO4 end of the substrate and generates 3'-OH, TdT can effectively elongate the 3'-OH end with deoxynucleotide adenine triphosphate (dATP) and produce a poly A tail, which can be detected by the poly T probes. Endo IV digests the AP site in poly T probes to generate a fluorescent signal and a new 3'-OH end, leading to the generation of exponential fluorescence signal amplification. The substrate for TdT elongation was optimized, and a limit of detection of 4.3 x 10(-3) U/L was achieved for ALP by the optimized substrate structure. This method can also detect ALP in the cell lysate of a single cell. This work has potential applications in disease diagnosis and biomedical detection. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of Xi'an Jiaotong University.
通讯机构：
[Haijun Lu; Rongtang Zhang; Nenghao Zhao] S;School of Civil Engineering and Architecture,Wuhan Polytechnic University,Wuhan 430023,China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
In the study of the impact of rainfall on the unsaturated soil slope, the changes of pore water pressure and effective stress are frequently analyzed, while the loading effect caused by the change of pore water content in unsaturated soils is often neglected. In order to realize the coupling effect of the above two, a coupling model involving pore water pressure and pore water gravity was established based on the principle of seepage-stress coupling in unsaturated soils. Through a case verification, the coupling effect of pore water pressure and pore water gravity on the unsaturated soil slope was analyzed from the aspects of effective saturation, pore water pressure, effective stress, displacement, and stability. At the same time, the results were compared with those considering only the pore water pressure. The results show that the slope stability is relatively poor considering the coupling of unsaturated pore water pressure and pore water gravity. Therefore, the gravity effect of pore water in unsaturated soils cannot be neglected when calculating the stability of unsaturated soil slope under rainfall condition.
