摘要：
<div class="mag_zhaiyao_sec"><p id="Par1" class="mag_zhaiyao_p">Cell migration is a fundamental biological process that plays a crucial role in both physiological and pathological conditions, and is largely influenced by the complex microenvironment, particularly the extracellular matrix (ECM), a macromolecular network that governs various cellular interactions. Extensive research has established that ECM-cell interactions are critical in multiple biological processes, with some directly regulating cell migration. Among ECM components, collagens stand out as key regulators of cell movement. However, existing reviews have provided only limited perspectives on the role of collagen-based biomaterials in directing migration across different cell populations. This gap in knowledge hinders a comprehensive understanding of collagen’s full potential. Drawing from systematic literature and our ongoing research, this review aims to summarize advancements over the past five years in the application of collagen-based biomaterials for modulating cell migration. The discussion primarily focuses on three pivotal cell types: stem cells, immune cells, and cancer cells. By shedding light on the functions, mechanisms, and therapeutic potential of collagen in cell migration, this review will contribute to the development of innovative collagen-based biomaterials with applications in wound healing and tissue regeneration.</div>
摘要：
In this study, bovine collagen peptide (BCP) decorated selenium nanoparticles (BCP-SeNPs) were prepared using BCP as a protective and reducing agent, and their structure, hypolipidemic and antitumor activities were investigated. The results of the various techniques including dynamic light scattering (DLS), transmission electron microscopy (TEM), energy dispersive x-ray (EDX) spectroscopy, x-ray photoelectron spectroscopy (XPS), circular dichroism (CD) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy confirmed the successful synthesis of BCP-SeNP. The polydispersity index (PDI) and zeta-potential analysis displayed that the PDI of BCP-SeNPs was about 0.12, smaller than BCP. The zeta-potential absolute value of BCP-SeNPs was found to be 34.2 mV, higher than BCP, suggesting that the stability of the BCP-SeNPs was better than BCP. Hypolipidemic performance evaluation indicated that binding capacity of BCP-SeNPs to bile salts was the highest when its concentration was 0.3 mM, and significantly higher than bare SeNPs, bovine serum albumin decorated SeNPs (BSA-SeNPs), and hyaluronic acid decorated SeNPs (HA-SeNPs). Moreover, the viability of HepG2 cells after BCP-SeNPs treatment was only 22.95%, which was considerably lower than that of bare SeNPs, BSA-SeNPs, and HA-SeNPs, showing better antitumor activity. These findings are expected to provide novel active ingredients that can be employed as functional foods and drugs.
通讯机构：
[Li, S ] W;[Wang, HB ] H;Wuhan Polytech Univ, Sch Chem & Environm Engn, Hubei Prov Key Lab Agr Waste Resource Utilizat, Wuhan, Hubei, Peoples R China.;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Resource Utilizat & Qual Control Cha, Xiaogan, Peoples R China.
关键词：
Fish collagen-derived peptides;Water-soluble;AIE;ESIPT;Schiff base;Metal ion detection
摘要：
Peptides, which are hydrolysis products of proteins, are commonly utilized as nutritional supplements in food due to their high bio-safety profile. In this study, we present a novel water-soluble fluorescent probe derived from fish collagen peptides, termed Sali-Pep. This probe exhibits light green fluorescence, characterized by both aggregate-induced-emission (AIE) and excited state intramolecular proton transfer (ESIPT) mechanisms, with emission peaks at 480 nm and 522 nm. Furthermore, Sali-Pep demonstrates a high sensitivity towards Cu 2+ ions via a “turn-off” fluorescence mechanism, and towards Zn 2+ ions through a “turn-on” fluorescence mechanism, both in a dose-dependent manner. The detection limits of Sali-Pep were 1.49 × 10 −7 M for Cu 2+ and 4.23 × 10 −7 M for Zn 2+ , respectively. In real environmental and biological samples, the recoveries of Cu 2+ and Zn 2+ ions ranged from 91.14 % to 108.61 %. These findings indicate that Sali-Pep is a promising fluorescent probe for the detection of Cu 2+ and Zn 2+ ions, combining both high biosafety and low environmental risk. Additionally, this study also show that collagen-derived peptides have the potential to serve as AIE sensing platforms for metal ion detection and fluorescent labeling.
Peptides, which are hydrolysis products of proteins, are commonly utilized as nutritional supplements in food due to their high bio-safety profile. In this study, we present a novel water-soluble fluorescent probe derived from fish collagen peptides, termed Sali-Pep. This probe exhibits light green fluorescence, characterized by both aggregate-induced-emission (AIE) and excited state intramolecular proton transfer (ESIPT) mechanisms, with emission peaks at 480 nm and 522 nm. Furthermore, Sali-Pep demonstrates a high sensitivity towards Cu 2+ ions via a “turn-off” fluorescence mechanism, and towards Zn 2+ ions through a “turn-on” fluorescence mechanism, both in a dose-dependent manner. The detection limits of Sali-Pep were 1.49 × 10 −7 M for Cu 2+ and 4.23 × 10 −7 M for Zn 2+ , respectively. In real environmental and biological samples, the recoveries of Cu 2+ and Zn 2+ ions ranged from 91.14 % to 108.61 %. These findings indicate that Sali-Pep is a promising fluorescent probe for the detection of Cu 2+ and Zn 2+ ions, combining both high biosafety and low environmental risk. Additionally, this study also show that collagen-derived peptides have the potential to serve as AIE sensing platforms for metal ion detection and fluorescent labeling.
通讯机构：
[Zhu, L ] W;[Qi, CB ] J;[Wang, HB ] H;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan 432000, Peoples R China.
摘要：
Stem cells serve as promising candidates for regenerative medicine. The migration capability of stem cells may determine the treatment efficacy. Currently, chemotaxis and thigmotaxis are two plausible approaches to regulate cell migration behaviors, with thigmotaxis requiring actual contact between the cell and an external substrate. Collagen, a main component of the extracellular matrix, is promising for regulating cell behaviors. However, few studies have focused on revealing the regulatory effect of collagen on cell migration, especially the relationship between collagen configuration and stem cell migration behavior. Collagen plays an essential role in many regenerative processes, highlighting the importance of using collagen to explore its regulatory effect on stem cell migration. Therefore, grass carp derived collagen configuration was flexibly controlled through heating in a water bath, cross-linking with a chemical strategy, and assembly. The migration behavior of adipose tissue-derived stromal cells was regulated. Results confirmed that migration capability increased with higher levels of cross-linking and assembly, while denaturation inhibited migration. Additionally, the expression level of F-actin was positively correlated with migration behavior under these conditions. In conclusion, we presented a simple yet effective approach for designing collagen-based biomaterials to precisely control stem cell migration processes, potentially enhancing the treatment efficacy of some diseases.
通讯机构：
[Zhang, JT ] W;[Wang, HB ] H;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan, Hubei, Peoples R China.
关键词：
Alginate;Collagen;Hemostasis
摘要：
Biomacromolecule-based hemostatic materials with biocompatibility and biodegradability have become a topic of significant research for the treatment of wound hemorrhage. Among available biomacromolecules, collagen and alginate are particularly promising. Although collagen and alginate composite materials have been developed, the impact of the spatial structures of collagen on the hemostatic properties of these materials remains to be fully understood. Collagen fibers, formed through self-assembly, share the same composition as collagen but exhibit distinct spatial structures. In this study, calcium alginate (CaAlg) membranes containing collagen (Col) or collagen fibers (Col-fiber) were fabricated. By adjusting the ratio of collagen to alginate, Col/CaAlg and Col-fiber/CaAlg composite membranes with favourable tensile strength and water retention ability were selected. The impact of collagen's spatial structures on the structures and properties of composite membranes was investigated, revealing that collagen fibers enhance the cytocompatibility, blood compatibility, and hemostatic performance of alginate membranes more effectively than collagen. Therefore, the Col-fiber/CaAlg membranes could be a promising candidate for hemostatic applications.
Biomacromolecule-based hemostatic materials with biocompatibility and biodegradability have become a topic of significant research for the treatment of wound hemorrhage. Among available biomacromolecules, collagen and alginate are particularly promising. Although collagen and alginate composite materials have been developed, the impact of the spatial structures of collagen on the hemostatic properties of these materials remains to be fully understood. Collagen fibers, formed through self-assembly, share the same composition as collagen but exhibit distinct spatial structures. In this study, calcium alginate (CaAlg) membranes containing collagen (Col) or collagen fibers (Col-fiber) were fabricated. By adjusting the ratio of collagen to alginate, Col/CaAlg and Col-fiber/CaAlg composite membranes with favourable tensile strength and water retention ability were selected. The impact of collagen's spatial structures on the structures and properties of composite membranes was investigated, revealing that collagen fibers enhance the cytocompatibility, blood compatibility, and hemostatic performance of alginate membranes more effectively than collagen. Therefore, the Col-fiber/CaAlg membranes could be a promising candidate for hemostatic applications.
摘要：
High stability of selenium nanoparticles (SeNPs) is crucial for preserving their biochemical properties. Currently, surface modification is one of the most effective routes to improve the stability of nanoparticles. Herein, we synthesized SeNPs through a mild reduction reaction at room temperature. Subsequently, the SeNPs were simultaneously coated with polyvinylpyrrolidone (PVP) and glucose to improve stability, the obtained composite was abbreviated as PG-SeNPs. Results demonstrated that PG-SeNPs displayed excellent stability without any coagulation even when stored for as long as 2 months. The hydrodynamic particle size remained almost uniform. After that, doxorubicin (DOX) was loaded onto the surface of PG-SeNPs via electrostatic interaction, resulting in PG-SeNPs@DOX, with a loading efficiency of 4.53 ± 0.3 %. In addition, the biocompatibility of PG-SeNPs was conducted and proven to be excellent. Consequences obtained in both in the in vitro and in vivo studies revealed that the PG-SeNPs@DOX could effectively kill the tumor cells and remarkably inhibit the growth of the transplanted tumor. We proposed that the antitumor efficacy obtained above was primarily dominated by the synergistic effect of both the reactive oxygen species induced by SeNPs and the released DOX. In summary, this study presents a straightforward and plausible method for producing highly stable SeNPs, and further confirms the antitumor effectiveness of PG-SeNPs@DOX.
High stability of selenium nanoparticles (SeNPs) is crucial for preserving their biochemical properties. Currently, surface modification is one of the most effective routes to improve the stability of nanoparticles. Herein, we synthesized SeNPs through a mild reduction reaction at room temperature. Subsequently, the SeNPs were simultaneously coated with polyvinylpyrrolidone (PVP) and glucose to improve stability, the obtained composite was abbreviated as PG-SeNPs. Results demonstrated that PG-SeNPs displayed excellent stability without any coagulation even when stored for as long as 2 months. The hydrodynamic particle size remained almost uniform. After that, doxorubicin (DOX) was loaded onto the surface of PG-SeNPs via electrostatic interaction, resulting in PG-SeNPs@DOX, with a loading efficiency of 4.53 ± 0.3 %. In addition, the biocompatibility of PG-SeNPs was conducted and proven to be excellent. Consequences obtained in both in the in vitro and in vivo studies revealed that the PG-SeNPs@DOX could effectively kill the tumor cells and remarkably inhibit the growth of the transplanted tumor. We proposed that the antitumor efficacy obtained above was primarily dominated by the synergistic effect of both the reactive oxygen species induced by SeNPs and the released DOX. In summary, this study presents a straightforward and plausible method for producing highly stable SeNPs, and further confirms the antitumor effectiveness of PG-SeNPs@DOX.
关键词：
Magnetic nanoparticles;Fish scale residues;Porous carbon;Peroxymonosulfate;Water treatment
摘要：
Peroxymonosulfate (PMS)-based Fenton-like oxidation has gained attention as a promising technology for wastewater remediation. However, its widespread application is constrained by the lack of high-performance and durable activators. In this study, a magnetic graphite-like porous carbon (MGPC) derived from fish scale residues was synthesized and demonstrated exceptional PMS activation performance, effectively degrading various persistent contaminants with minimal iron leaching (<0.8 mg L⁻ 1 ). Notably, MGPC achieved high degradation rates for sulfamethoxazole (0.13 min⁻ 1 g⁻ 1 L), methylene blue (0.65 min⁻ 1 g⁻ 1 L), tetracycline (0.19 min⁻ 1 g⁻ 1 L), and rhodamine B (1.07 min⁻ 1 g⁻ 1 L), outperforming many state-of-the-art activators. This enhanced activity is attributed to the synergistic effects of multiple active components, including Fe 3 C, Fe 3 O 4 , and a nitrogen-doped carbon matrix. Additionally, the MGPC/PMS system exhibited strong resistance to interference from background constituents and maintained broad pH adaptability (3–9). Impressively, over 95 % of methylene blue was degraded after five consecutive cycles. The MGPC powders were easily recovered using an external magnetic field, eliminating the need for complex separation processes. Radical quenching experiments, in-situ electron paramagnetic resonance (EPR) analysis, and chronoamperometry confirmed that the MGPC/PMS system operates through two non-radical pathways: 1 O 2 -mediated oxidation and direct electron transfer. This innovative approach to designing efficient PMS activators from environmental waste offers a sustainable strategy for environmental pollution control.
Peroxymonosulfate (PMS)-based Fenton-like oxidation has gained attention as a promising technology for wastewater remediation. However, its widespread application is constrained by the lack of high-performance and durable activators. In this study, a magnetic graphite-like porous carbon (MGPC) derived from fish scale residues was synthesized and demonstrated exceptional PMS activation performance, effectively degrading various persistent contaminants with minimal iron leaching (<0.8 mg L⁻ 1 ). Notably, MGPC achieved high degradation rates for sulfamethoxazole (0.13 min⁻ 1 g⁻ 1 L), methylene blue (0.65 min⁻ 1 g⁻ 1 L), tetracycline (0.19 min⁻ 1 g⁻ 1 L), and rhodamine B (1.07 min⁻ 1 g⁻ 1 L), outperforming many state-of-the-art activators. This enhanced activity is attributed to the synergistic effects of multiple active components, including Fe 3 C, Fe 3 O 4 , and a nitrogen-doped carbon matrix. Additionally, the MGPC/PMS system exhibited strong resistance to interference from background constituents and maintained broad pH adaptability (3–9). Impressively, over 95 % of methylene blue was degraded after five consecutive cycles. The MGPC powders were easily recovered using an external magnetic field, eliminating the need for complex separation processes. Radical quenching experiments, in-situ electron paramagnetic resonance (EPR) analysis, and chronoamperometry confirmed that the MGPC/PMS system operates through two non-radical pathways: 1 O 2 -mediated oxidation and direct electron transfer. This innovative approach to designing efficient PMS activators from environmental waste offers a sustainable strategy for environmental pollution control.
作者：
Haofei Xu;Yang Liu;Longxue Qiu;Antonio Lorenzo Masa Mbomio Mangue;Juntao Zhang;...
期刊：
Collagen and Leather,2025年7(1):1-19 ISSN：2097-1419
通讯作者：
Zhang, JT;Wang, HB
作者机构：
[Chengzhi Xu; Antonio Lorenzo Masa Mbomio Mangue; Benmei Wei; Longxue Qiu; Yang Liu; Lian Zhu; Juntao Zhang; Haofei Xu; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Wang, Haibo; Haibo Wang] Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan, Hubei, Peoples R China.;[Evgeny A. Shirshin] MV Lomonosov Moscow State Univ, Dept Phys, Leninskie Gory 1-2, Moscow 119991, Russia.
通讯机构：
[Zhang, JT ] W;[Wang, HB ] H;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan, Hubei, Peoples R China.
关键词：
Collagen;Hemostasis;Biomacromolecule
摘要：
Traumatic wounds are the prevalent scenarios encountered in battleground and emergency rooms. The rapid and effective hemostasis is imperative for life saving in these scenarios, for which the development of high-efficiency and biocompatible hemostatic materials is essential. Due to its excellent hemostatic property and biocompatibility, collagen has emerged as an ideal component of hemostatic materials. Furthermore, the properties of collagen-based hemostatic materials could be improved by the integration of other biomacromolecules, such as alginate, cellulose derivatives, and chitosan derivatives. Therefore, more and more novel hemostatic materials with exceptional hemostatic properties have been developed. This review aims to overview recent progress of collagen-based hemostatic materials. Firstly, the hemostatic mechanism of collagen was introduced. Secondly, various forms of collagen-based hemostatic materials, such as hydrogels, sponges, and powders, were highlighted. Thirdly, composite hemostatic materials of collagen and other biomacromolecules were overviewed. Finally, the outlook of collagen-based hemostatic materials was discussed.
通讯机构：
[Wei, BM ] W;[Wang, HB ] H;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan, Hubei, Peoples R China.
摘要：
A significant focus of carbon dot research is on enhancing the fluorescence emission performance of biomimetic carbon dots to improve their application value in practical analysis. In this study, fish scales were used as a precursor, and citric acid was introduced to improve the quantum yield of carbon dots. The results showed that under 350 nm excitation, citric acid-modified carbon dots (CDs-FS/CA) exhibited a maximum fluorescence emission of 411 nm, and the emission behavior was independent of the excitation wavelength, with a quantum yield of 35.5%. This high quantum yield could be attributed to the presence of citric acid and the participation of hydroxyapatite in fish scales. The CDs-FS/CA had a moderate degree of graphitization, smaller and more concentrated particle size distribution, and a high proportion of pyrrole N. They showed good fluorescence performance through the synergistic effect of surface state sp2 C and different N-doped surface states. A good linear relationship in the range of 0-50 mu mol L-1 was obtained using CDs-FS/CA for trace detection of quercetin, with a limit of detection of 3.8 nmol L-1, and good recovery in actual sample detection. These results offer a reference for enhancing the quantum yield of CDs obtained from alternative biomass sources and indicate the encouraging commercial feasibility of CDs derived from waste biomass for detecting trace amounts of quercetin. Through modification, this work significantly enhances the quantum yield of carbon dots derived from fish scales and applies them for trace detection of quercetin.
期刊：
European Polymer Journal,2024年215:113240 ISSN：0014-3057
通讯作者：
Wang, HB
作者机构：
[Xu, Chengzhi; Sui, Peishan; Wei, Benmei; Liu, Yang; Xu, Haofei; Zhang, Juntao; Zheng, Mingming] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Wang, Haibo; Zhu, Lian] Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan, Hubei, Peoples R China.;[Zheng, Mingming] Chinese Acad Agr Sci, Key Lab Oilseeds Proc, Hubei Key Lab Lipid Chem & Nutr, Oil Crops Res Inst,Minist Agr, Wuhan, Hubei, Peoples R China.
通讯机构：
[Wang, HB ] H;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan, Hubei, Peoples R China.
关键词：
Itaconic anhydride;Collagen;Hydrogelation
摘要：
Based on the bioconjugation engineering, the structures and properties of collagen could be optimized, which is attractive for the development of novel collagen-based biomaterials. Here, itaconic anhydride was used to modify the amino groups in collagen to prepare the itaconic anhydride modified collagen (CITA) with different grafting density. The grafting density of CITAs was detected by TNBS assay. The integrity and thermostability of triple helical structure in CITAs were revealed by CD. Hydrophilicity of CITAs was studied by isoelectric point and water contact angles analysis. The concentration of CITA solution could be improved to 30 mg/mL under physiological environment. The hydrogelation of CITA was realized by photopolymerization, based on the introduction of double bonds. The storage modulus (G ') of CITA hydrogel could be improved to 680 Pa, compared with 67 Pa of Col hydrogel. The improved hemostasis property and antibacterial property of CITAs were confirmed, and CITAs also possessed good biocompatibility. This work provided a novel artificially modified collagen with optimized properties, which could be used for 3D bioprinting and fabrication of the in situ hemostatic hydrogel system.
摘要：
Mineral elements including calcium, iron, and zinc play crucial roles in human health. Their deficiency causes public health risk globally. Commercial mineral supplements have limitations; therefore, alternatives with better solubility, bioavailability, and safety are needed. Chelates of food-derived peptides and mineral elements exhibit advantages in terms of stability, absorption rate, and safety. However, low binding efficiency limits their application. Extensive studies have focused on understanding and enhancing the chelating activity of food-derived peptides with mineral elements. This includes obtaining peptides with high chelating activity, elucidating interaction mechanisms, optimizing chelation conditions, and developing techniques to enhance the chelating activity. This review provides a comprehensive theoretical basis for the development and utilization of food-derived peptide-mineral element chelates in the food industry. Efforts to address the challenge of low binding rates between peptides and mineral elements have yielded promising results. Optimization of peptide sources, enzymatic hydrolysis processes, and purification schemes have helped in obtaining peptides with high chelating activity. The understanding of interaction mechanisms has been enhanced through advanced separation techniques and molecular simulation calculations. Optimizing chelation process conditions, including pH and temperature, can help in achieving high binding rates. Methods including phosphorylation modification and ultrasonic treatment can enhance the chelating activity.
摘要：
An economical and sustainable approach was proposed for the fabrication of two-end-open carbon nanorods within three-dimensional interconnected hierarchical porous structure, which was utilized to modify the glass carbon electrode(GCE) for detecting ascorbic acid (AA) for the first time. The constructed PCNRs/GCE displayed a statistical linear concentration range of 8-500 µM and 0.5-9.615 mM for AA with a detection limit of 0.11 µmol L− 1 based on a signal-to-noise ratio (S/N) of 3 with the ideal amount of modification. It featured superior stability, repeatability, selectivity, and anti-interference capability. An electrochemical kinetic investigation revealed the superior electrochemical performance could be ascribed to the rapid ion transport and electron transfer between electrode surface and AA triggering by surface adsorption control. It is related to the large surface area, excellent conductivity, and abundant electroactive sites/defects, which are attracted to its unique structure. The determination feasibility was evaluated in juice with recovery rates from 99.8–100.4%% and relative standard deviation of below 3%. The PCNRs demonstrate significant potential as an effective material for ascorbic acid electrochemical sensor systems.
1. Porous carbon nanorods (PCNRs) were prepared via a green and sustainable method.
2. The materials demonstrated three-dimensional interconnected hierarchical pore channels.
3. Electrochemical oxidation mechanism of AA on the PCNRs/GCE surface was investigated.
4. PCNRs/GCE sensor demonstrated enhanced electrochemical behavior.
摘要：
Food-derived peptides (FPs) are bioactive molecules produced from dietary proteins through enzymatic hydrolysis or fermentation. These peptides exhibit various biological activities. However, their efficacy largely depends on bioavailability, the ability to cross absorption barriers, and reach target sites within the body. This review addresses key issues in FP absorption, including barriers, pathways, influencing factors, and strategies to enhance absorption. The biochemical and physical barriers to FP absorption include pH variations, enzymes, unstirred water layer, mucus layer, and intestinal epithelial cells. FPs enter the bloodstream via four main pathways: carrier-mediated transport, endocytosis, paracellular, and passive diffusion. The barrier-crossing efficiency depends on the structural properties and state of FPs and coexisting substances. Absorption efficiency can be significantly improved with permeability enhancers, nano-delivery systems, and chemical modifications. These insights provide a scientific basis and practical guidance for optimizing the bioactivity and health benefits of food-derived peptides.
Food-derived peptides (FPs) are bioactive molecules produced from dietary proteins through enzymatic hydrolysis or fermentation. These peptides exhibit various biological activities. However, their efficacy largely depends on bioavailability, the ability to cross absorption barriers, and reach target sites within the body. This review addresses key issues in FP absorption, including barriers, pathways, influencing factors, and strategies to enhance absorption. The biochemical and physical barriers to FP absorption include pH variations, enzymes, unstirred water layer, mucus layer, and intestinal epithelial cells. FPs enter the bloodstream via four main pathways: carrier-mediated transport, endocytosis, paracellular, and passive diffusion. The barrier-crossing efficiency depends on the structural properties and state of FPs and coexisting substances. Absorption efficiency can be significantly improved with permeability enhancers, nano-delivery systems, and chemical modifications. These insights provide a scientific basis and practical guidance for optimizing the bioactivity and health benefits of food-derived peptides.
期刊：
European Polymer Journal,2024年217:113292 ISSN：0014-3057
通讯作者：
Zhu, L;Wang, HB;Wen, W
作者机构：
[Xu, Yu-Ling; Zhu, Lian; Liu, Jia-Ling; Chen, Shuang; Wei, Ben-Mei] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Wang, Hai-Bo] Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan 432000, Peoples R China.;[Wen, Wei] Hubei Univ, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 4300062, Peoples R China.
通讯机构：
[Zhu, L ] W;[Wen, W ; Wang, HB ] H;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan 432000, Peoples R China.;Hubei Univ, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 4300062, Peoples R China.
关键词：
Collagen;Migration;Adhesion
摘要：
Cell adhesion and migration behaviors are widely involved in physiological processes, and can be mediated by various factors. Collagen is one representative constituents of extracellular matrix, possessing advantages including excellent biocompatibility, low immunogenicity, and ease to be engineered, which makes collagen be promising biomaterial for regulating cell behaviors. Furthermore, the biofunction of collagen is closely correlated with its configuration. Whereas, current studies on collagen-based biomaterials for regulating cell adhesion and migration are rare, let alone the exploration of the ralationship between the configurations and these two behaviors. (Human umbilical vein endothelial cells, HUVEC) plays significant roles in wounds healing and cancer progression. Therefore, we aimed at disclosing the relationships between the adhesion/migration capability and the collagen origin, structure, and molecular state. In summary, results clearly revealed that denaturation disfavors the adhesion/migration of HUVEC cells, while assembly promotes. The expression levels of F-actin and vinculin, together with the quantity of filopodia were positively correlated with the two processes. Finnaly, our study may provide directions for the further designing of collagen-based biomaterials in the treatment of these kinds of severe diseases.
Cell adhesion and migration behaviors are widely involved in physiological processes, and can be mediated by various factors. Collagen is one representative constituents of extracellular matrix, possessing advantages including excellent biocompatibility, low immunogenicity, and ease to be engineered, which makes collagen be promising biomaterial for regulating cell behaviors. Furthermore, the biofunction of collagen is closely correlated with its configuration. Whereas, current studies on collagen-based biomaterials for regulating cell adhesion and migration are rare, let alone the exploration of the ralationship between the configurations and these two behaviors. (Human umbilical vein endothelial cells, HUVEC) plays significant roles in wounds healing and cancer progression. Therefore, we aimed at disclosing the relationships between the adhesion/migration capability and the collagen origin, structure, and molecular state. In summary, results clearly revealed that denaturation disfavors the adhesion/migration of HUVEC cells, while assembly promotes. The expression levels of F-actin and vinculin, together with the quantity of filopodia were positively correlated with the two processes. Finnaly, our study may provide directions for the further designing of collagen-based biomaterials in the treatment of these kinds of severe diseases.
通讯机构：
[Wang, HB ] H;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan, Hubei, Peoples R China.
关键词：
Fish scale collagen hydrolysate;Plastein reaction;Antioxidant properties;Reactive oxygen species
摘要：
An important area of focus for developing antioxidant collagen hydrolysates (peptides) involves enhancing the antioxidant properties of collagen hydrolysates. In this study, fish scale collagen hydrolysate (FH) was used as the raw material for plastein reaction. Various enzymes, including papain, alcalase, flavourzyme, and the combination of alcalase and flavourzyme, were employed for this purpose. The plastein reaction significantly improved the thermal stability, chemical antioxidant activity, and capacity to scavenge cellular reactive oxygen species of FH. Notably, the plastein reaction catalyzed by alcalase exhibited the most significant improvement, increasing the hydroxyl radical scavenging rate from 72.3 to 93.4% and restoring the viability of the oxidative stress-induced HepG2 cell model from 50.8 +/- 1.7 to 74.9 +/- 1.7%. During the plastein reaction, condensation and hydrolysis reactions occurred simultaneously, with condensation being the dominant process. These reactions, along with physical aggregation, facilitated the formation of larger yet more concentrated collagen peptide aggregates, leading to increased exposure of hydrophobic groups. This enhanced the uptake of collagen hydrolysates by the cells and contributed to the enhancement of their antioxidant properties. Thus, the plastein reaction is an effective method for enhancing the antioxidant properties of collagen hydrolysates, with its simplicity of operation and promising application potential.
摘要：
The uncontrollable migration of cancer cells is one of the reasons for the poor prognosis. The interaction between collagen and cellular processes is usually dominated by the full triple-helix structure of collagen. However, it remains to be explored how the collagen configuration affects the migration behaviors of cancer cells. Michigan cancer foundation-7 cells are chosen to study this relationship. Meanwhile, the grass carp is one of the representative fish species in Hubei and is virtually unaffected by zoonotic diseases. Therefore, grass carp-derived collagen is prepared and used to modulate the migration behaviors. The collagen sample is pretreated via several procedures including the hydrothermal process and EDC/NHS, respectively. The underlying mechanisms affecting migration behavior are explored by tuning the degree of denaturation, assembly states, and cross-linking conditions of collagen. Results demonstrate that moderate cross-linking and assembly contribute to the migration processes, while denaturation significantly suppresses migration. Of particular note is the positive correlation of F-actin expression levels with these phenomena. More importantly, the hydrophilic and hydrophobic properties of the collagen surface also affect the migration process. In summary, the study may provide insights to flexibly tune the migration behavior of cancer cells, which may be promising in inhibiting tumor metastasis. The relationship between the configuration of grass crap-derived collagen and migration behavior of cancerous cells is studied in this article. Results confirm that denaturation inhibits migration, while cross-linking and assembly contribute to the migration of michigan cancer foundation-7 cells. This study may provide an opportunity for exploring the utilization value of collagen-based biomaterials for inhibiting tumor metastasis.image (c) 2024 WILEY-VCH GmbH
通讯机构：
[Wei, BM; Wang, HB ] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;Hubei Engn Univ, Coll Life Sci & Technol, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan 432000, Peoples R China.
关键词：
Cell adhesion;Collagen;Signal peptide
摘要：
The signal peptides GVMGFO and GFOGER exhibit differential binding affinities towards Michigan Cancer Foundation-7 (MCF-7) breast cancer cells and HT-1080 human fibrosarcoma cells, respectively, which in turn modulate the cell adhesion properties of natural collagen. GVMGFO demonstrates a more potent interaction with discoidin domain receptor 1(DDR1)-expressing MCF-7 cells, whereas GFOGER preferentially binds to the integrin alpha 2 beta 1 present on HT-1080 cells. The integration of GVMGFO into natural collagen through direct doping or crosslinking markedly enhances its association with MCF-7 cells, especially when optimal peptide concentrations and blending ratios are utilized, indicating a synergistic effect. This augmented adhesion is attributed to specific binding at the DDR1-collagen interface, facilitated by a constellation of amino acids within the collagen scaffold engaging with the DDR1 discoidin (DS) domain through polar interactions and hydrogen bonding. Conversely, the incorporation of GFOGER into natural collagen through co-assembling or crosslinking leads to a progressive increase in adherence to HT-1080 cells, as evidenced by the peptide's affinity for integrin alpha 2 beta 1. These findings advance the design of collagen-based biomaterials for targeted cellular interactions in the medical, pharmaceutical, and enhance our understanding of the molecular mechanisms governing peptide-collagen mediated cell adhesion processes.
