摘要：
<jats:p><jats:italic>Pseudomonas</jats:italic> is one of the most diverse bacterial genera identified in the environment. Genome sequence analysis has indicated that this genus can be clustered into three lineages and ten groups. Each group can adopt different mechanisms to thrive under zinc-depleted or high-zinc conditions, two environments that are frequently encountered during their environmental propagation. The response of three prominent <jats:italic>Pseudomonas</jats:italic> strains (<jats:italic>Pseudomonas aeruginosa</jats:italic> PAO1, <jats:italic>Pseudomonas putida</jats:italic> KT2440, and <jats:italic>Pseudomonas fluorescens</jats:italic> ATCC 13525<jats:sup>T</jats:sup>) to minimal inhibitory concentrations of zinc were compared using RNA-seq and ultra-performance liquid chromatography–tandem mass spectrometry analysis. Results demonstrated that the three strains shared only minimal similarity at the transcriptional level. Only four genes responsible for zinc efflux were commonly upregulated. <jats:italic>P. aeruginosa</jats:italic> PAO1 specifically downregulated the operons involved in siderophore synthesis and the genes that encode ribosomal protein, while upregulated the genes associated with antibiotic efflux and cell envelope biosynthesis. The membrane transporters in <jats:italic>P. putida</jats:italic> KT2440 were globally downregulated, indicating changes in cell permeability. Compared with <jats:italic>P. aeruginosa</jats:italic> PAO1 and <jats:italic>P. putida</jats:italic> KT2440, the most remarkable transcriptional variation in <jats:italic>P. fluorescens</jats:italic> ATCC 13525<jats:sup>T</jats:sup> is the significant downregulation of the type VI secretion system. Metabolite quantitative analysis showed that low concentrations of the metabolites involved in central carbon metabolism and amino acid synthesis were detected in the three strains. In summary, the cellular responses of the three strains under high-zinc condition is quite divergent. Although similar metal efflux systems were upregulated, the three strains employed different pathways to reduce zinc intrusion. In addition, zinc treatment can increase the difficulties of scavenging <jats:italic>P. aeruginosa</jats:italic> from its colonization area, and reduce the competitiveness of <jats:italic>P. fluorescens</jats:italic> in microbiota.</jats:p>
摘要：
Polysaccharides are considered to be promising candidates for non-viral gene delivery because of their molecular diversity, which can be modified to fine-tune their physicochemical properties. In this work, transcriptional activator protein (TAT) functionalized PEI grafted polysaccharide polymer (PRBP) was prepared by using rice bran polysaccharide as the starting material, and characterized by various methods. The potential of TAT functionalized PRBP (PRBP-TAT) as gene vector was studied in vitro, including DNA loading capacity, DNA protection ability and biocompatibility. The cell uptake and transfection efficiency of the PRBP-TAT/pDNA polyplexes were studied. The results showed that PRBP-TAT could completely condense DNA at N/P 2. The PRBP-TAT/pDNA polyplexes could protect DNA from degrading by DNase and were efficiently internalized by cells. Biocompatibility result showed that PRBP-TAT had no significant cytotoxicity and effect on cell proliferation. At low N/P ratios of 1-3.5, PRBP-TAT showed higher transfection efficiency than PEI30k and PEI30k-grafted rice bran polysaccharide. PRBP-TAT and PEI showed the highest transfection efficiency of 42.8% and 28.1% when pDNA is 2 microg and N/P ratio is 1.5, respectively, while PRBP showed the highest transfection efficiency of 37.3% at N/P 2.5. These results indicate that PTA is a promising candidate vector for safe and efficient gene delivery.
摘要：
Xanthine oxidase (XOD) catalyses the oxidation of hypoxanthine into xanthine and xanthine into uric acid. The enzyme plays a key role in the purine metabolic pathway. Despite the presence of different XODs in prokaryotes, the functional and structural knowledge of prokaryotic XODs remain limited (compared with their well-known eukaryotic counterparts), thereby hindering their biochemical analysis and industrial application. Using genetic and biochemical analyses, we identified and characterised recombinant XOD (CcXOD(AB)) from Cellulosimicrobium cellulans ATCC21606. Bioinformatics analysis suggests that CcXOD(AB) shares low amino acid sequence identities with other XODs. The purified enzyme exhibits the maximum activity at 55 degrees C and pH 8.0. In addition, CcXOD(AB) exhibits moderate thermostability and retains 80.65 % of the original activity after 30 min of incubation at 60 degrees C. Ca2+ has a slight inhibitory effect, whereas Co2+ and Mn2+ have a strong inhibitory effect on XODAB activity. In particular, low Ba2+ and Mg2+ concentrations have no effect, whereas high Mg2+ (>= 10 mM) and Ba2+ (>= 2 mM) concentrations show an inhibitory effect on enzyme activity. The K-m and V-max values for xanthine are 131.29 +/- 11.09 mu mol.L-1 and 15.23 +/- 0.65 mu mol.L(-1)min(-1), respectively. Results indicate that CcXOD(AB) is a novel enzyme with potential industrial application.
摘要：
A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons. In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin. The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 . The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 . Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges. Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution. When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively. The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants. PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment. The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 . The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.
期刊：
LANCET GLOBAL HEALTH,2020年8(8):e1038-e1060 ISSN：2214-109X
通讯作者：
Wiens, Kirsten E.
作者机构：
[Welgan, Catherine A.; Feigin, Valery L.; Mayala, Benjamin K.; Blacker, Brigette F.; Munro, Sandra B.; Murray, Christoper J. L.; Dandona, Lalit; Cormier, Natalie Maria; Dharmaratne, Samath Dhamminda; Mokdad, Ali H.; Dandona, Rakhi; LeGrand, Kate E.; Deshpande, Aniruddha; Henry, Nathaniel J.; Johnson, Kimberly B.; Hay, Simon I.; Baumann, Mathew M.; Schaeffer, Lauren E.; Lindstedt, Paulina A.; Ross, Jennifer M.; Levine, Aubrey J.; Reiner, Robert C., Jr.; Weaver, Marcia; Wiens, Kirsten E.] Univ Washington, Inst Hlth Metr & Evaluat, Seattle, WA 98195 USA.;[Murray, Christoper J. L.; Dharmaratne, Samath Dhamminda; Mokdad, Ali H.; Dandona, Rakhi; Hay, Simon I.; Sartorius, Benn; Reiner, Robert C., Jr.; Weaver, Marcia] Univ Washington, Sch Med, Dept Hlth Metr Sci, Seattle, WA USA.;[Ross, Jennifer M.; Kochhar, Sonali] Univ Washington, Dept Global Hlth, Seattle, WA 98195 USA.;[Massenburg, Benjamin Ballard] Univ Washington, Div Plast & Reconstruct Surg, Seattle, WA 98195 USA.;[Somayaji, Ranjani; Ross, Jennifer M.] Univ Washington, Dept Med, Seattle, WA USA.
通讯机构：
[Wiens, Kirsten E.] U;Univ Washington, Inst Hlth Metr & Evaluat, Seattle, WA 98195 USA.
摘要：
Background Oral rehydration solution (ORS) is a form of oral rehydration therapy (ORT) for diarrhoea that has the potential to drastically reduce child mortality; yet, according to UNICEF estimates, less than half of children younger than 5 years with diarrhoea in low-income and middle-income countries (LMICs) received ORS in 2016. A variety of recommended home fluids (RHF) exist as alternative forms of ORT; however, it is unclear whether RHF prevent child mortality. Previous studies have shown considerable variation between countries in ORS and RHF use, but subnational variation is unknown. This study aims to produce high-resolution geospatial estimates of relative and absolute coverage of ORS, RHF, and ORT (use of either ORS or RHF) in LMICs. Methods We used a Bayesian geostatistical model including 15 spatial covariates and data from 385 household surveys across 94 LMICs to estimate annual proportions of children younger than 5 years of age with diarrhoea who received ORS or RHF (or both) on continuous continent-wide surfaces in 2000-17, and aggregated results to policy-relevant administrative units. Additionally, we analysed geographical inequality in coverage across administrative units and estimated the number of diarrhoeal deaths averted by increased coverage over the study period. Uncertainty in the mean coverage estimates was calculated by taking 250 draws from the posterior joint distribution of the model and creating uncertainty intervals (UIs) with the 2 center dot 5th and 97 center dot 5th percentiles of those 250 draws. Findings While ORS use among children with diarrhoea increased in some countries from 2000 to 2017, coverage remained below 50% in the majority (62 center dot 6%; 12 417 of 19 823) of second administrative-level units and an estimated 6 519 000 children (95% UI 5 254 000-7 733 000) with diarrhoea were not treated with any form of ORT in 2017. Increases in ORS use corresponded with declines in RHF in many locations, resulting in relatively constant overall ORT coverage from 2000 to 2017. Although ORS was uniformly distributed subnationally in some countries, within-country geographical inequalities persisted in others; 11 countries had at least a 50% difference in one of their units compared with the country mean. Increases in ORS use over time were correlated with declines in RHF use and in diarrhoeal mortality in many locations, and an estimated 52 230 diarrhoeal deaths (36 910-68 860) were averted by scaling up of ORS coverage between 2000 and 2017. Finally, we identified key subnational areas in Colombia, Nigeria, and Sudan as examples of where diarrhoeal mortality remains higher than average, while ORS coverage remains lower than average. Interpretation To our knowledge, this study is the first to produce and map subnational estimates of ORS, RHF, and ORT coverage and attributable child diarrhoeal deaths across LMICs from 2000 to 2017, allowing for tracking progress over time. Our novel results, combined with detailed subnational estimates of diarrhoeal morbidity and mortality, can support subnational needs assessments aimed at furthering policy makers' understanding of within-country disparities. Over 50 years after the discovery that led to this simple, cheap, and life-saving therapy, large gains in reducing mortality could still be made by reducing geographical inequalities in ORS coverage. Copyright (c) 2020 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license.
作者机构：
[Liu, Jianfang; Wei, Lei; Duan, Haitao; Jin, Yongliang; Liu, Lian; Sun, Xianming; Jia, Dan; Chen, Song; Li, Jian] Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Peoples R China.;[Liu, Jianfang] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Sun, Xianming] Wuhan Inst Technol, Sch Mat Sci & Engn, Wuhan 430073, Peoples R China.
通讯机构：
[Li, Jian] W;Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Peoples R China.
关键词：
motor oil;oil condition evaluation;on-board diagnostic system
摘要：
The condition of the motor oil in civilian cars is difficult to monitor; hence, we propose a method to evaluate the degree of degradation of motor oil using an on-board diagnostic (OBD) system. Three civilian cars and four motor oils (containing mineral oils and synthetic oils) were subjected to five groups of road tests under urban traffic and high-way conditions. The operation information, oil service time, mileage, engine operation time, idle time of the engine, and number of start-ups of the engine were obtained using the proposed OBD system. Physiochemical properties and changes in the components of motor oils during road tests were analyzed in laboratory. The theoretical model of the comprehensive indicators of driving parameters and oil properties were established. The proposed method was successfully applied to different cars, motor oils, and operating conditions in road tests. All the theoretical models had high accuracy and precision. Herein, we provide a method to monitor the oil condition with real-time driving parameters and provide a reference for end users to change their motor oil reasonably.
关键词：
anthraquinone;azo dye;low-voltage;pulse electrolysis;Reactive Blue 19
摘要：
<jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>Wastewater produced by the textile industry containing azo dyes and anthraquinone dyes is significant source of pollution to the environment and is toxic for aquatic life. To overcome the high‐energy cost of traditional electrochemical oxidation, a custom‐built power supply device for the degradation of anthraquinone and azo dyes by low voltage of 15.0–20.0V pulsed discharge was investigated. Titanium coated with mixed oxide (Ti/IrO<jats:sub>2</jats:sub>‐RuO<jats:sub>2</jats:sub>‐SnO<jats:sub>2</jats:sub>) plates and pure titanium plates were used as the anode and cathode, respectively, for the generation of chlorine in the dye solution. For the anthraquinone dye Reactive Blue 19, 60.0% of the chemical oxygen demand (COD) and 22.0% of the total organic carbon (TOC) were removed using this system. A comparison of the direct current electrolysis and pulsed discharge revealed that using the pulsed discharge method reduced the energy cost by 68.6%. UV–visible, LC‐MS, and GC‐MS were used to identify the intermediate compounds formed during the degradation of Reactive Blue 19. The results indicate that in the process of oxidation by chlorine/hypochlorite, the chromophore group was first oxidized to –NH<jats:sub>2</jats:sub>, followed by decolorization via chlorination of the aromatic rings. The results confirm that low‐voltage pulse electrolysis can be used for the degradation of industrial dyes in waste effluents.</jats:p></jats:sec><jats:sec><jats:title>Practitioner points</jats:title><jats:p>
<jats:list list-type="bullet">
<jats:list-item><jats:p>Low‐voltage pulse electrolysis can be used for the degradation of industrial dyes and/or dyes in waste effluents.</jats:p></jats:list-item>
<jats:list-item><jats:p>For anionic dye Reactive Blue 19, 60.0% of COD and 22.0% of TOC were removed using low‐voltage (20.0V) pulse electrolysis.</jats:p></jats:list-item>
<jats:list-item><jats:p>The pulsed discharge method reduced the energy cost of this degradation process by 68.6% compared with direct current electrolysis.</jats:p></jats:list-item>
<jats:list-item><jats:p>The intermediate compounds formed during the degradation of Reactive Blue 19 were confirmed by UV–visible spectroscopy, LC‐MS, and GC‐MS.</jats:p></jats:list-item>
</jats:list>
</jats:p></jats:sec>
关键词：
climate-smart agriculture;crayfish-rice coculture;mixed crop-fish system
摘要：
Agriculture affects and is affected by climate change. The development of climate-smart agriculture (CSA) is an effective way for agricultural production to cope with climate change. The rice-crayfish coculture system is a combination of the concept of climate-smart agriculture with the experience of traditional Chinese mixed crop-fish systems. This paper reviews the advantages and potential risks of rice-crayfish coculture with respect to climate, ecological environment and economic benefits, and summarises the successful management strategies and operation experiences during the implementation of this CSA practice in Qianjiang City. We also introduce various mixed crop-fish systems in China and suggest the potential of using wetland resources to develop mixed crop-fish systems and CSA.
通讯机构：
[Li, Chun] D;Departments of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77054, United States.;Center for RNAi and Non-Coding RNA, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77054, United States.
摘要：
The tyrosine kinase receptor EphB4 is frequently overexpressed in ovarian and other solid tumors and is involved in interactions between tumor cells and the tumor microenvironment, contributing to metastasis. Trans-interaction between EphB4 and its membrane-bound ligand ephrin B2 (EFNB2) mediates bi-directional signaling: forward EFNB2-to-EphB4 signaling suppresses tumor cell proliferation, while reverse EphB4-to-EFNB2 signaling stimulates the invasive and angiogenic properties of endothelial cells. Currently, no small molecule-based, dual-function, EphB4-binding peptides are available. Here, we report our discovery of a bi-directional ephrin agonist peptide, BIDEN-AP which, when selectively internalized via receptor-mediated endocytosis, suppressed invasion and epithelial-mesenchymal transition of ovarian cancer cells. BIDEN-AP also inhibited endothelial migration and tube formation. In vivo, BIDEN-AP and its nanoconjugate CCPM-BIDEN-AP significantly reduced growth of orthotopic ovarian tumors, with CCPM-BIDEN-AP displaying greater antitumor potency than BIDEN-AP. Both BIDEN-AP and CCPM-BIDEN-AP compromised angiogenesis by downregulating epithelial-mesenchymal transition and angiogenic pathways. Thus, we report a novel EphB4-based therapeutic approach against ovarian cancer.
摘要：
<jats:title>Abstract</jats:title><jats:p>The tyrosine kinase receptor EphB4 is frequently overexpressed in ovarian and other solid tumors and is involved in interactions between tumor cells and the tumor microenvironment, contributing to metastasis. Trans-interaction between EphB4 and its membrane-bound ligand ephrin B2 (EFNB2) mediates bi-directional signaling: forward EFNB2-to-EphB4 signaling suppresses tumor cell proliferation, while reverse EphB4-to-EFNB2 signaling stimulates the invasive and angiogenic properties of endothelial cells. Currently, no small molecule–based, dual-function, EphB4-binding peptides are available. Here, we report our discovery of a <jats:underline>bi</jats:underline>-<jats:underline>d</jats:underline>irectional <jats:underline>e</jats:underline>phri<jats:underline>n a</jats:underline>gonist <jats:underline>p</jats:underline>eptide, BIDEN-AP which, when selectively internalized <jats:italic>via</jats:italic> receptor-mediated endocytosis, suppressed invasion and epithelial-mesenchymal transition of ovarian cancer cells. BIDEN-AP also inhibited endothelial migration and tube formation. <jats:italic>In vivo</jats:italic>, BIDEN-AP and its nanoconjugate CCPM-BIDEN-AP significantly reduced growth of orthotopic ovarian tumors, with CCPM-BIDEN-AP displaying greater antitumor potency than BIDEN-AP. Both BIDEN-AP and CCPM-BIDEN-AP compromised angiogenesis by downregulating epithelial-mesenchymal transition and angiogenic pathways. Thus, we report a novel EphB4-based therapeutic approach against ovarian cancer.</jats:p>
摘要：
Metal complexes, as a type of potential non-virus gene carriers, have gained much attention due to their properties of high charge density and unique three-dimensional structure. This study investigated the potential of polyethyleneimine (PEI)-modified rice bran polysaccharide-Fe(III) complex (PEI-PI) as a safe gene delivery system and explored the effect of Fe(III) on the efficiency of gene transfection mediated by PEI modified rice bran polysaccharide (PEI-P) and PEI-PI. Gel retardation assay was used to study the DNA binding and protection capability, MTT assay was performed to evaluate the biocompatibility, and PEI-PI complex-mediated EGFP gene transfection was studied in vitro. Results showed the PEI-PI could induce DNA condensation and protect DNA from degradation by DNase I at a low weight ratio (vector/DNA) of 2. At the same weight ratio, PEI exhibited the strongest DNA binding capability but PEI-PI exhibited the highest gene transfection efficiency among all carrier systems. Compared with the PEI-P + Fe(III) system, PEI-PI not only had a more significant capability to condense DNA but also presented higher gene transfection efficiency. Moreover, PEI-PI exhibited no obvious cytotoxicity to cells. This work provides a strategy for the design and development of gene vectors based on PI complexes. (C) 2019 Elsevier B.V. All rights reserved.
摘要：
This study used response surface methodology to determine the optimal conditions for extraction of polysaccharides from Pyracantha. fortuneana (PSPF), and studied the mechanism of PSPF-inducing apoptosis in human ovarian carcinoma Skov3 cells. Response surface methodology (RSM) were adopted to extract PSPF. The maximum value of polysaccharide yield was obtained under these optimal conditions. PSPF had good potential as an antioxidant. Exposure of cells to PSPF resulted in cytotoxicity through the induction of apoptosis, and the reactive oxygen species were increased, mitochondrial membrane potential decreased, DNA damage (detected as gamma- H2AX and RAD51 foci) was observed in Skov3 cells. In addition, PSPF could induce apoptosis of cancer cells. Therefore, PSPF should be explored as novel potential antioxidants and an anti-tumor drug in a clinical setting.
摘要：
The rhizome of P. japonicus var. major, one of the important herbs in Traditional Chinese medicine (TCM), has been used as tonic and hemostatic drugs in Tujia and Miao ethnic groups of China for thousand years. In this study, comparative metabolite and transcriptome analysis of rhizome nodes and internodes of wild P. japonicus var. major was performed to reveal their different roles in the biosynthesis of triterpene saponins. The results showed that the node was the crucial section for the synthesis of ginsenosides in the rhizome. The content of oleanane-type ginsenosides in the node was much higher than those in the internode. Most isoprenoid biosynthesis-related genes were highly expressed in the node. And, candidate UDP-glycosyltransferase (UGT) genes were also found to be differentially expressed between node and internode. Our study will provide a better understanding of the metabolism of ginsenosides in the rhizome of P. japonicus var. major.
摘要：
A SiOx coating prepared via plasma-enhanced chemical vapor deposition (PECVD) techniquewas used as a transition layer between polylactic acid (PLA) and chitosan (CS) to form a three-layer composite film of PLA/SiOx/CS. The effect of oxygen plasma irradiation time (0 s, 30s and 60s) on SiOx surface and PLA-CS interface was examined based on the contact angle, pull-off test and scanning electron microscope (SEM). The mechanical, barrier and antibacterial properties of PLA/SiOx/CS films were also investigated. The results showed that oxygen plasma irradiation improved the hydrophilicity of the SiOx surface and the adhesive strength between SiOx-CS of PLA/ SiOx/CS films in a time-dependent manner. SEM examination revealed a gap between PLA/CS layers, but the interfacial separation among layers in PLA/SiOx/CS films disappeared as the transition layer of SiOx and oxygen plasma irradiation (60s) intensified. Notably, the oxygen barrier property and antibacterial activity of PLA/ SiOx/CS films were dramatically enhanced. Additionally, moisture resistance was slightly decreased following the incorporation of the CS coating compared with the PLA film. The tensile strength of the composite film also increased with the number of layers, while elongation at break decreased. The prepared PLA/SiOx/CS films with multifunction are promising applied in food packaging as biobased materials. (C) 2020 Elsevier B.V. All rights reserved.
摘要：
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Intracellular bacteria, especially <jats:styled-content style="fixed-case"><jats:italic>Mycobacterium tuberculosis</jats:italic></jats:styled-content>, are important pathogenic microorganisms that endanger human health. Purified and synthesized cecropin A‐magainin 2 (CAMA‐syn) can exhibit a higher antibacterial activity and lower cytotoxicity. To enhance such antimicrobial potential, it would be desirable to deliver CAMA‐syn expressed in lung epithelial cells by an adenovirus vector using gene therapy.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>A549 cells <jats:italic>in vitro</jats:italic> and lung epithelial cells <jats:italic>in vivo</jats:italic> were used to express CAMA‐syn by transducing recombinant adenovirus Ad‐SPC‐CAMA/GFP, and the expression of CAMA‐syn was determined by a reverse transcriptase‐polymerase reaction (RT‐PCR) and immunofluorescence. The antimicrobial activity in cells was investigated by colony‐forming rate and growth curve. Forty Kunming mice of a Bacillus Calmette‐Guerin (BCG) infection animal model were randomly divided into three groups: adenoviruses delivery of Ad‐SPC‐CAMA/GFP, Ad‐CMV‐CAMA/GFP and empty‐virus Ad‐CMV‐GFP. The expression of CAMA‐syn in mice was confirmed by RT‐PCR and immunofluorescence. After tracheal injection of adenoviral vector for 3 days, lungs from the mouse model were extracted and homogenized for detection of colony‐forming efficiency.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>CAMA‐syn expressed in lung epithelial cells A549 conferred antimicrobial activity against a series of bacteria, including <jats:italic>Salmonella abortusovis</jats:italic> and BCG. The results obtained <jats:italic>in vivo</jats:italic> showed that the colony‐forming rate of Ad‐SPC‐CAMA/GFP (74.54%) and Ad‐CMV‐CAMA/GFP (62.31%) transduced into mice was significantly lower than that of the control group.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Lung epithelial‐specific expression of antimicrobial peptide CAMA‐syn mediated by adenovirus suppressed the growth of intracellular bacteria, providing a promising approach for the control of refractory intracellular infection.</jats:p></jats:sec>
