作者机构：
[Jingcheng Dai; Jiangke Yang] School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China;[Dingxin Wen; Yong Yang] State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, 430062, China;[Hao Li; Jingpeng Yang; Xiongfei Rao] Tobacco Research Institute of Hubei Province, Wuhan , Hubei, 430030, China;[Jiangke Yang] Pilot Base of Food Microbial Resources Utilization of Hubei Province, College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430024, China;[Chunlei Yang] Tobacco Research Institute of Hubei Province, Wuhan , Hubei, 430030, China. ycl193737@163.com
通讯机构：
[Chunlei Yang; Jun Yu] T;Tobacco Research Institute of Hubei Province, Wuhan
, Hubei, China<&wdkj&>Tobacco Research Institute of Hubei Province, Wuhan
, Hubei, China
摘要：
BACKGROUND: Hydrogen sulfide (H(2)S) is a novel signaling molecule involved in the growth and development of plants and their response to stress. However, the involvement of H(2)S in promoting the growth and development of tobacco plants is still unclear. RESULTS: In this study, we explored the effect of pre-soaking or irrigating the roots of tobacco plants with 0.0, 2.0, 4.0, 6.0, and 8.0mM of sodium hydrosulfide (NaHS) on endogenous H(2)S production, antioxidant enzymatic and cysteine desulfhydrase activities, seed germination, agronomic traits, photosynthetic pigments contents, and root vigor. The results revealed that exogenous NaHS treatment could significantly promote endogenous H(2)S production by inducing gene expression of D/L-CD and the activities of D/L-CD enzymes. Additionally, a significant increase in the agronomic traits and the contents of photosynthetic pigments, and no significant difference in carotenoid content among tobacco plants treated with 0.0 to 8.0mM of NaHS was observed. Additionally, a significant increase in the germination speed, dry weight, and vigor of tobacco seeds, whereas no significant effect on the percentage of seed germination was observed on NaHS treatment. Furthermore, NaHS treatment could significantly increase the activity of superoxide dismutase (SOD) and peroxidase (POD) enzymes, which reduces damage due to oxidative stress by maintaining reactive oxygen species homeostasis. CONCLUSIONS: These results would aid in enhancing our understanding of the involvement of H(2)S, a novel signaling molecule to promote the growth and development of tobacco plants.
作者机构：
[Qian, Shenxian; Chen, Zhenzhen; Chen, Can; Shi, Pengfei; Li, Yiwei] Department of Hematology, Affiliated Hangzhou First People's Hospital, West Lake University, School of Medicine, Hangzhou, China;[Li, TongYu] Department of Hematology, Ningbo First Hospital, Ningbo, China;[Li, Yun] Team of Neonatal & Infant Development, Health and Nutrition, NDHN, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China;[Li, Yun] Kindstar Global Precision Medicine Institute, Wuhan, China;[Li, Yun] Department of Scientific Research Project, Wuhan Kindstar Medical Laboratory Co., Ltd., Wuhan, China
通讯机构：
[Yun Li] T;[Shenxian Qian] D;Department of Hematology, Affiliated Hangzhou First People's Hospital, West Lake University, School of Medicine, Hangzhou, China<&wdkj&>Team of Neonatal & Infant Development, Health and Nutrition, NDHN, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China<&wdkj&>Kindstar Global Precision Medicine Institute, Wuhan, China<&wdkj&>Department of Scientific Research Project, Wuhan Kindstar Medical Laboratory Co., Ltd., Wuhan, China
关键词：
B cell immune;Biomarker;DLBCL;GPX4;Single cells
摘要：
At present, GPX4's role in the occurrence and development of diffuse large B lymphoma (DLBCL) is rarely reported. This study's purpose is to explore GPX4's significance in the diagnosis, treatment, and pathological mechanisms of DLBCL. The TIMER 2.0, GEPIA, and GEO databases were used to analyze GPX4's expression levels in DLBCL tissue, peripheral blood, and single cells, and evaluate its potential performance as a therapeutic and diagnostic marker. Cell experiments validate GPX4's role in DLBCL cells. And revealed the potential mechanism of GPX4's action from three aspects: immunity, pathogenic gene expression, and protein interaction. The results indicate that GPX4 can be used as a biomarker for treatment and diagnosis (FC>1.5, P<0.05, AUC>0.8, KM-P value<0.05). In single cell data, GPX4 also showed high expression in immune cells. Besides, cell experiments have confirmed that GPX4's high expression can inhibit DLBCL cells' proliferation. Meanwhile, we found a negative correlation between GPX4 and the 16 core DLBCL's pathogenic genes, and a significant negative correlation with immune B cell infiltration. In summary, GPX4 can serve as a potential therapeutic and diagnostic marker for DLBCL. GPX4's high expression can lead to a good prognosis in DLBCL patients, which may be related to its inhibition of cancer cell proliferation, high expression of key pathogenic genes, and infiltration of immune B cells.
作者机构：
[Duan, Linlin; Duan, Haitao; Li, Guozheng; Duan, HT; Jia, Dan] Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Applic, Wuhan 430030, Peoples R China.;[Duan, Linlin; Duan, Haitao; Li, Guozheng; Duan, HT; Jia, Dan] Hubei Longzhong Lab, Xiangyang 441000, Peoples R China.;[Liu, Jianfang] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430030, Peoples R China.;[Cheng, Bingxue] Tsinghua Univ, State Key Lab Tribol Adv Equipment, Beijing 100084, Peoples R China.
通讯机构：
[Duan, HT ] W;Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Applic, Wuhan 430030, Peoples R China.;Hubei Longzhong Lab, Xiangyang 441000, Peoples R China.
摘要：
In this work, a self-assembled hybrid phosphate nanoflower (HPN) containing Ba3(PO4)2 and NaBaPO4 was synthesized via BaCO3 primary crystals inducement. A ball-on-disc reciprocating tribometer was used to explore the tribological behavior of HPN as an additive for polyalphaolefin 8 (PAO8) on titanium alloy. Compared with the neat PAO8, after being lubricated with PAO8 containing HPN, the friction coefficient and wear rate decreased by 74.98% and 99.89%, respectively. The tribofilm at the friction interface was characterized and conformed by SEM, EDS, XPS, and cross-sectional TEM. As an additive to PAO8, HPN can participate in the formation of tribofilm and exhibit superior friction-reducing and anti-wear properties for titanium alloy. It was demonstrated that due to the P-O-Ti bonds, HPN can easily adsorb and deposit at the friction interface to form a tribofilm against wear. Besides, the simulation experiments showed that the repulsive force at the solid-liquid interface between HPN and oil molecules is the key to friction reduction and lubrication, and the comparative tribological experiments of different types of base oils were performed to verify the results of molecular dynamics.
摘要：
Identifying functional enzymes for the catalysis of specific biochemical reactions is a major bottleneck in the de novo design of biosynthesis and biodegradation pathways. Conventional methods based on microbial screening and functional metagenomics require long verification periods and incur high experimental costs; recent data-driven methods apply only to a few common substrates. To enable rapid and high-throughput identification of enzymes for complex and less-studied substrates, we propose a robust enzyme's substrate promiscuity prediction model based on positive unlabeled learning. Using this model, we identified 15 new degrading enzymes specific for the mycotoxins ochratoxin A and zearalenone, of which six could degrade >90% mycotoxin content within 3 h. We anticipate that this model will serve as a useful tool for identifying new functional enzymes and understanding the nature of biocatalysis, thereby advancing the fields of synthetic biology, metabolic engineering, and pollutant biodegradation.
期刊：
FRONTIERS IN IMMUNOLOGY,2024年15:1324552 ISSN：1664-3224
作者机构：
Koç University, Research Center for Translational Medicine, İstanbul, Türkiye;Hubei Province Engineering Research Center of Healthy Food, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hebei Province, China
摘要：
Air pollution plays an important role in the mortality and morbidity of chronic airway diseases, such as asthma and chronic obstructive pulmonary disease (COPD). Particulate matter (PM) is a significant fraction of air pollutants, and studies have demonstrated that it can cause airway inflammation and injury. The airway epithelium forms the first barrier of defense against inhaled toxicants, such as PM. Airway epithelial cells clear airways from inhaled irritants and orchestrate the inflammatory response of airways to these irritants by secreting various lipid mediators, growth factors, chemokines, and cytokines. Studies suggest that PM plays an important role in the pathogenesis of chronic airway diseases by impairing mucociliary function, deteriorating epithelial barrier integrity, and inducing the production of inflammatory mediators while modulating the proliferation and death of airway epithelial cells. Furthermore, PM can modulate epithelial plasticity and airway remodeling, which play central roles in asthma and COPD. This review focuses on the effects of PM on airway injury and epithelial plasticity, and the underlying mechanisms involving mucociliary activity, epithelial barrier function, airway inflammation, epithelial-mesenchymal transition, mesenchymalepithelial transition, and airway remodeling.
摘要：
Botrytis cinerea is a necrotrophic fungal plant pathogen that causes grey mould and rot diseases in many crops. Here, we show that the B. cinerea BcCrh4 transglycosylase is secreted during plant infection and induces plant cell death and pattern-triggered immunity (PTI), fulfilling the characteristics of a cell death-inducing protein (CDIP). The CDIP activity of BcCrh4 is independent of the transglycosylase enzymatic activity, it takes place in the apoplast and does not involve the receptor-like kinases BAK1 and SOBIR1. During saprophytic growth, BcCrh4 is localized in the endoplasmic reticulumand in vacuoles, but during plant infection, it accumulates in infection cushions (ICs) and is then secreted to the apoplast. Two domains within the BcCrh4 protein determine the CDIP activities: a 20aa domain at the N' end activates intense cell death and PTI, while a stretch of 52aa in the middle of the protein induces a weaker response and suppresses the activity of the 20aa N' domain. Deletion of bccrh4 affected fungal development and IC formation in particular, resulting in reduced virulence. Collectively, our findings demonstrate that BcCrh4 is required for fungal development and pathogenicity, and hint at a dual mechanism that balances the virulence activity of this, and potentially other CDIPs.
摘要：
Introduction: The nutritional value of duck meat is well acknowledged due to its low cholesterol and high protein content. Nevertheless, the impacts of deep-frying and baking on its quality characteristics are not extensively documented in literature. Methods: The objective of this study is to examine the effects of deep-frying, pre-boilingdeep-frying, baking, and pre-boiling-baking on the quality attributes, water distribution, microstructure, and flavor characteristics of duck jerky. Results and discussion: The findings revealed that the deep-frying group had better quality attributes than the baking, pre-boiling-deep-frying, and preboiling-baking groups. The deepfried duck jerky had a higher a* value (4.25) and a lower b* value (5.87), with a more appropriate texture profile, and had the highest comprehensive impression score (5.84). Moreover, the drying rate was faster, and the intensity of the free water and oil signal was significantly elevated in the deep-frying group. The microstructure results indicated that the muscle fibers in the deep-frying group were closely packed, whereas those in the baking group were relatively loose. Furthermore, the GC-IMS test revealed that the deep-fried duck jerky had a wider range of volatile flavor compounds, including 11 unique compounds that were only found in this particular product.
作者机构：
[Qiuqi Lei; Min Zhou] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Xiaoyan Sun; Qinyi Chen; Dandan Song; Hongxun Wang] College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China;Author to whom correspondence should be addressed.;[Limei Wang] College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
通讯机构：
[Limei Wang] C;College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Background: Trapa bispinosa shells (TBs) and its flesh (TBf) have been recognized for their medicinal properties, including antioxidant, antitumor, and immunomodulatory effects. Despite these benefits, TBs are often discarded as waste material, and their applications remain to be further explored. Methods: In this study, we optimized the solid-state fermentation process of Ganoderma sinense (GS) with TBs using a response surface experiment methodology to obtain the fermented production with the highest water extract rate and DPPH free radical scavenging activity. We prepared and characterized pre-fermentation purified polysaccharides (P1) and post-fermentation purified polysaccharides (P2). Alcoholic extracts before (AE1) and after (AE2) fermentation were analyzed for active components such as polyphenols and flavonoids using UPLC-QTOF-MS/MS (ultra-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry). Mouse macrophages (RAW 264.7) were employed to compare the immune-stimulating ability of polysaccharides and the antioxidant activity of AE1 and AE2. Results: Optimal fermentation conditions comprised a duration of 2 days, a temperature of 14 °C, and a humidity of 77%. The peak water extract yield and DPPH free radical scavenging rate of the water extract from TBs fermented by GS were observed under these conditions. The enhanced activity may be attributed to changes in the polysaccharide structure and the components of the alcoholic extract. The P2 treatment group indicated more secretion of RAW 264.7 cells of NO, iNOS, IL-2, IL-10, and TNF-α than P1, which shows that the polysaccharides demonstrated increased immune-stimulating ability, with their effect linked to the NF-кB pathway. Moreover, the results of the AE2 treatment group indicated that secretion of RAW 264.7 cells of T-AOC and T-SOD increased and MDA decreased, which shows that the alcoholic extract demonstrated enhanced antioxidant activity, with its effect linked to the Nrf2/Keap1-ARE pathway. Conclusions: Biphasic fermentation of Trapa bispinosa shells by Ganoderma sinense could change the composition and structure of the polysaccharides and the composition of the alcoholic extract, which could increase the products’ immunomodulatory and antioxidant activity.
作者机构：
[舒少华; 刘和平; 刘金鑫; 唐静; 苏振佳] College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China;[朱闻君] School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
通讯机构：
[Shu, S.-H.] C;College of Plant Science and Technology, China
摘要：
This study investigated the effects of 1 g L-1 ethylene immersion treatment for 1 min on the storage quality, antioxidant capacity and ethylene signal transduction pathway of fresh-cut lotus roots. Phenylalanine ammonia-lyase and polyphenol oxidase activities were increased in ethylene treatment slices, and total phenolic content was increased. The activities of peroxidase, catalase and superoxide dismutase were increased after ethylene treatment, as well as the expression of related genes (NnPOD, NnSOD, NnCAT) was upregulated. The rate of superoxide anion radical and hydroxyl radical production in ethylene treatment fresh-cut lotus roots was inhibited, the hydrogen peroxide content was reduced, and the degree of membrane lipid peroxidation was decreased, which delayed browning during the early storage. However, ethylene treatment inhibited microbial growth and improved 1,1-Diphenyl-2-picrylhydrazyl scavenging capacity only during the early storage, pro-moted respiration and accelerated the decline of hardness and soluble solids content throughout the storage period. At the molecular level, ethylene treatment overall upregulated the transcription of NnmetK, NnACO, NnETR, NnEIN3, and NnERF genes and promoted ethylene biosynthesis and signal transduction. This finding may be related to the senescence of fresh-cut lotus roots during late storage. These results suggest that ethylene treatment improved the antioxidant system, reduced the accumulation of reactive oxygen species, and effectively delayed the early browning of fresh-cut lotus roots, while promoting late senescence.
通讯机构：
[Ting Min] C;College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China
关键词：
Fresh-cut Chinese water chestnut;Ethylene;Browning;Reactive oxygen species (ROS)
摘要：
The phytohormone ethylene responds positively to abiotic stress, but its effect on quality deterioration of freshcut Chinese water chestnuts (CWCs) caused by mechanical damage remains unclear. Here, the effects of single or combination treatments of ethephon (ET) and 1-methylcyclopropene (1-MCP) on fresh-cut CWCs during storage at 10 degrees C for 5 d were investigated. The results indicated that ET treatment was most effective in maintaining CWCs' visual quality, followed by 1-MCP preceding ET treatment, 1-MCP treatment, and 1-MCP following ET treatment. Compared with 1-MCP treatment group, ET-treated fruit exhibited lower expressions of CwPAL, CwCHS1 and CwCHI1 and decreased activity of phenylalanine ammonia-lyase activity to inhibit flavonoid accumulation but higher catalase and superoxide dismutase and peroxidase (POD) activities and lower malonaldehyde content to maintain reactive oxygen species balance and suppress microbial growth. Nevertheless, ET treatment promoted fruit weight loss and CO2 concentration in the package, which was reversed by the preceding 1-MCP application rather than the subsequent 1-MCP treatment. 1-MCP followed by ET treatment not only inhibited the levels of flavonoid compounds and oxidation catalyzed by POD compared with the post-1-MCP treatment, but also reduced H2O2 production and microbial spoilage with a higher catalase activity. These results show that ET could maintain the quality of fresh-cut CWC by boosting antioxidant capacity, inhibiting microbial growth and suppressing enzymatic generation of browning substrates. In addition, compared with ET treatment, 1-MCP followed by ET treatment, despite showing weaker antioxidant enhancement, could inhibit the senescence of fresh-cut CWCs, suggesting its suitability for quality control of fresh-cut CWCs.
