作者机构：
Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of SubtropicalAgricult;Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University,Wuhan 430023;Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agricul
会议名称：
4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)
会议时间：
2009-07-04
会议地点：
广州
会议论文集名称：
4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)论文集
摘要：
Effects of dietary protein level on intramuscular fat content and its fatty acid composition in lean and obese genotype finishing pigs were studied. Twenty four Crossbred (Duroc×Landrace×Large White) barrows (49.1±4.9kg of BW) and eighteen Ningxiang barrows (61.6±4.1kg of BW) were equally randomly assigned to two groups and fed diets I (13.89％ CP) and diet II (15.33％ CP), respectively. The pigs were slaughtered after 46 days. Longissimus dorsi muscle (LD) and semitendinosus muscle (ST) samples were taken and the intramuscular fat (IMF) content as well as its fatty acid compositions was determined. There was no significant difference in IMF content both in LD and ST between breed, within breed or between muscles, when diet I was fed. The percentage of oleic acid (C18:1) and linoleic acid (C18:2) in LD of Crossbred was lower (P<0.01) and higher (P<0.01) significantly than that of Ningxiang, respectively, when diet I was fed. There was no significant difference (P>0.05) in fatty acid compositions in ST between breeds when diet I was fed. When diet II was fed, the IMF amount of LD in Crossbred was significantly lower (P<0.01) than that in Ningxiang and significantly lower (P<0.05) than that of its own ST. Ratio of stearic acid (C18:0), C18:1 and C18:2 in LD of Crossbred was significantly higher (P<0.01), lower (P<0.01) and higher (P<0.01) than that of Ningxiang pig, respectively. Ratio of palmitic acid (C16:0), C18:0, C18:1 and C18:2 in ST of Crossbred was significantly lower (P<0.01), higher (P<0.01), lower (P<0.01) and higher (P<0.01) than that of Ningxiang pig. Ratio of C18:1 in LD of Crossbred was significantly lower (P<0.01) than that of its own ST while ratio of C18:0 and C18:1 in LD of Ningxiang pig was significantly higher (P<0.05) and lower (P<0.05) than that of its own ST, respectively. These results indicate that breed, protein level of diet and muscle types have effects on IMF content and its fatty acid composition and that different muscle types of different breeds responded differently in IMF content and fatty acid composition to the small change in dietary protein level.
摘要：
通过在日粮中添加不同水平多糖复合物(mixture of chitosan and saccharicterpenin,MCS),研究其对鲫鱼(Carassius auratus)躯体生化组成、脏器指数及白细胞数目的影响.研究结果表明:3个MCS添加组水分含量、粗蛋白含量较空白对照组有所降低(P＞0.05),但粗脂肪含量则有不同程度增加(P＞0.05);3个MCS添加组灰分含量均低于空白对照组,且0.25%MCS组显著低于空白对照组(P＜0.05);各组间钙、磷含量无显著差异(P＞0.05).添加不同水平的MCS对鲫鱼脏体比、肝胰脏指数和胆指数均具有不同程度的降低作用,其中0.50%MCS组脏体比极显著(P＜0.01)、肝胰脏指数显著(P＜0.05)低于空白对照组;3个MCS添加组胆指数均极显著低于空白对照组(P＜0.01),1.00%MCS组胆指数也显著低于0.25%MCS组(P＜0.05),且随MCS添加量的增加胆指数呈现下降趋势;各组间头肾指数、脾脏指数差异不显著(P＞0.05).各组间血液白细胞数目差异不显著(P＞0.05).
摘要：
Lutein supplements are often used to pigment and enrich layer chicken eggs. This experiment was conduced to compare the bioavailability of free and esterified lutein, by depletion method. Forty chickens were randomly divided into two groups. After 2 weeks of washout period, when all birds were fed the same low lutein basal diet, the two groups were fed for another 2 weeks with diets supplemented with free lutein or esterified lutein. Two experimental diets were supplemented with the same amount of 15 mg lutein/kg. On day 0 (baseline) and days 3, 7, and 14, birds fasting morning plasma samples were collected and stored for lutein analysis by high performance liquid chromatography method (HPLC). Results showed that: 1) Plasma lutein concentration increased dramatically after feeding free or esterified lutein; 2) Plasma lutein level in birds fed the diet supplemented with free lutein significantly differed from those fed esterified lutein on day 3. 3) There were no significant differences in plasma lutein levels between free lutein and esterified lutein on days 7 and 14. In conclusion, the lutein bioavailability from free lutein or esterified lutein supplements was comparable.
摘要：
Voltage-gated sodium channels (VGSCs) are important channels which participate in many physiological functions. Whether VGSCs can be modulated by changes in osmotality in trigeminal ganglion (TG) neurons remains unknown. In this study, by using whole-cell patch clamp techniques, we tested the effects of hypo- and hypertonicity on VGSCs in cultured TG neurons. Our data show that tetrodotoxin-resistant sodium Current (TTX-R current) was inhibited in the presence of hypo- and hypertonic solutions. In hypertonic solutions both voltage-dependent activation and inactivation curves shifted to the hyperpolarizing direction, while in hypotonic solutions only inactivation curve shifted to the hyperpolarizing direction. Transient Receptor Potential Vanilloid 4 (TRPV4) receptor activator mimicked the inhibition of TTX-R current by hypotonicity and the inhibition by hypotonicity was markedly attenuated by TRPV4 receptor blocker and in TRPV4(-/-) mice TG neurons. We also demonstrate that the inhibition of PKA selectively attenuated hypotonicity-induced inhibition, whereas antagonism of PLC and PI3K selectively attenuated hypertonicity-induced inhibition. We conclude that although hypo- and hypertonicity have similar effect on VGSCs, receptor and intracellular signaling pathways are different for hypo- versus hypertonicity-induced inhibition of TTX-R current. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
作者机构：
Key Laboratory for Agro-ecological Processes in Subtropical Region ,Institute of SubtropicalAgricult;Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University,Wuhan 430023;Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA;Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 43002
会议名称：
4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)
会议时间：
2009-07-04
会议地点：
广州
会议论文集名称：
4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)论文集
摘要：
Two experiments were conducted to evaluate the effects of dietary supplementation with astragalus polysaccharide (APS) growth performance, apparent ileal digestibilities (AID) of amino acids (AA), and their serum concentrations in early-weaned piglets. In Exp.1, sixty pigs were weaned at 21 days of age (BW = 7.35 ± 0.23 kg) and allocated to three treatments (20 pigs/treatment), representing supplementing 0.0％ (control), 0.02％ colistin (antibiotic), or 0.1％ APS to a corn- and soybean meal-based diet. Average daily gain (ADG), average daily feed intake (ADFI) and feed/gain ratio (F/G) were measured weekly. Blood samples were obtained from five pigs selected randomly from each treatment for the measurement of serum free AA concentrations on Days 7, 14 and 28. In Exp.2, twelve pigs were weaned at 21 day of age (BW = 7.64 ± 0.71 kg), assigned to three treatment groups as in Exp. 1, and surgically fitted with a simple T-cannula at the terminal ileum. Ileal digesta samples were obtained for the measurement of AID of AA on Days 7, 14 and 28. Dietary APS did not affect ADFI, but enhanced (P < 0.05) ADG by 11％ and 4.4％, and improved F/G by 5.6％ and 8.4％, respectively, compared with the control and antibiotic groups. Addition of APS to the diet increased AID and serum concentrations of most nutritionally essential and nonessential AA (including arginine, proline, glutamate, lysine, methionine, tryptophan, and threonine) on Days 14 and 28. Circulating levels of total AA were affected by the age of pigs and treatment × time interaction. Collectively, these findings indicate that APS may ameliorate the digestive and absorptive function and regulate AA metabolism to beneficially increase the entry of dietary AA into the systemic circulation, which provide a mechanism to explain the growth-promoting effect of APS in early-weaned piglets.
