作者机构：
[Gao, Weilan] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Cheng, Shuiyuan; Li, Shuyi; Li, Shuyao; Zhu, Zhenzhou; Gao, Weilan; Zhang, Na; Zhu, Shiyu] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pr, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.;[Barba, Francisco J.] Univ Valencia, Prevent Med & Publ Hlth, Food Sci Toxicol & Forens Med Dept, Fac Pharm, Avda Vicent Andres Estelles, Valencia 46100, Spain.
通讯机构：
[Shuyi Li] A;[Francisco J. Barba] P;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, 46100 Burjassot, València, Spain<&wdkj&>Authors to whom correspondence should be addressed.
关键词：
pulsed electric fields;selenium-enriched tea;selenium;polysaccharide;antioxidant activity
关键词：
Chinese quince;Sequential removal;Cell wall polysaccharides;Lignin structure
摘要：
The cross-linking between lignin and cell wall polysaccharides is one of recalcitrance to extract pectin and hemicellulose. In this work, water-, chelator-, Na2CO3-soluble pectin (WSP, CSP, NSP) and alkali-soluble hemicellulose (ASH) were sequentially removed, milled wood lignins (WMWL, CMWL, NMWL and KMWL) were isolated from Chinese quince. Quantitative chemical analysis techniques (e.g., HPAEC, FT-IR, GPC, Py-GC/MS, TG and 2D NMR) were used for characterizing the lignin fractions. Results indicate that there are tight linkages between arabinose and lignin. The molecular weight and thermal stability decreased as different types of pectin (WSP, CSP and NSP) were removed. Py-GC/MS and 2D NMR data showed that the major interunit beta-O-4' aryl ether bonds of lignin (61.07 %-72.53 %) and the S/G ratios (0.56-2.68) were changed. It was revealed that Chinese quince pectin and hemicellulose polysaccharides have more covalent crosslinks with S-type lignin from secondary wall S2 than G-type lignin. These results will contribute to realizing the mechanisms of cell wall polysaccharides isolation and be beneficial for the potential application of Chinese quince fruits in food and other industries.
摘要：
以天然高硒糙米与低硒糙米为原料，采用分级提取法提取糙米蛋白并对其进行结构表征，通过生物信息学技术结合蛋白组学技术探究蛋白的生物学意义。结果表明：高硒糙米和低硒糙米4种蛋白（清蛋白、球蛋白、醇溶蛋白和谷蛋白）中谷蛋白的蛋白质含量（86.39%）以及硒含量（363.80μg/100 g）最高；与低硒谷蛋白相比，高硒谷蛋白没有产生新的化学键，但其α-螺旋含量减少，无规则卷曲含量增多，蛋氨酸和游离巯基含量降低。Protein Ontology（GO）功能注释表明2种蛋白在生物过程、细胞成分及分子功能中，蛋白数最多的二级功能相同；与低硒谷蛋白不同的是，高硒谷蛋白Kyoto Encyclopedia of Proteins and Genomes（KEGG）通路注释具有环境信息处理通路。硒强化使糙米谷蛋白提取物中蛋白质含量、硒含量增加，谷蛋白结构发生了一定的变化，高硒谷蛋白与低硒谷蛋白其潜在的功能有一定差异。
作者机构：
[Li, Shuyi; Zhu, Zhenzhou; Chen, Xujun; Wang, Feifei; Chen, Zhe; Ming, Shanxiu] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pr, Sch Modern Ind Selenium Sci & Engn, Wuhan 430205, Peoples R China.;[Deng, Shaonan] Hubei Nanbai Shengtainongye Co Ltd, Enshi 445000, Peoples R China.;[Marszalek, Krystian] Prof Waclaw Browski Inst Agr & Food Biotechnol, Dept Fruit & Vegetable Prod Technol, State Res Inst, 36 Rakowiecka St, PL-02532 Warsaw, Poland.;[Marszalek, Krystian] Univ Rzeszow, Coll Nat Sci, Inst Food Technol & Nutr, Dept Food Technol & Human Nutr, Zelwerowicza 2D, PL-35601 Rzeszow, Poland.;[Zhang, Wenxiang] King Abdullah Univ Sci & Technol, Water Desalinat & Reuse Res Ctr, Biol & Environm Sci & Engn Div, Riyadh 11543, Saudi Arabia.
通讯机构：
[Zhenzhou Zhu; Francisco J. Barba] A;Authors to whom correspondence should be addressed.<&wdkj&>Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, University of Valencia, 46100 Valencia, Spain<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing Technology, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430205, China
作者机构：
[Guo Yuhao; Cao Yan; Wang Feifei] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Wang Feifei] Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China.;[Wang Feifei; Zhang Chengwu] Minist Educ, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn, Guangzhou 510632, Peoples R China.;[Zhang Chengwu] Jinan Univ, Dept Ecol, Guangzhou 510632, Peoples R China.;[Zhang Chengwu] Jinan Univ, Res Ctr Hydrobiol, Guangzhou 510632, Peoples R China.
通讯机构：
[Chengwu Zhang] E;Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou, China<&wdkj&>Department of Ecology and Research Center for Hydrobiology, Jinan University, Guangzhou, China
摘要：
Filamentous microalgae from genus Tribonema are promising sustainable sources of omega-7 palmitoleic acid, but their ability to accumulate this compound varies among species and depends on the initial nitrogen concentration (INC) supply. In this study, the palmitoleic acid accumulation capacities of five Tribonema species were examined under three different INCs to select the alga species with the highest production. Results showed that a high INC was associated with increased palmitoleic acid accumulation but led to decreased biomass concentration in all tested species. In particular, T. minus grown at 18 mmol L~(-1) INC had the highest palmitoleic acid content (20.72% of dry weight) and productivity (90.88 mg L~(-1) d~(-1)). The combination of alkali metal freezing precipitation (AMFP) and urea complexation successfully isolated and enriched palmitoleic acid from T. minus and obtained a purity of 80.11% and a yield of 7.39 g (100 g)-1 of algal powder. The compound was identified as (9Z)-hexadecenoic acid (C16:1 ω-7). Antibacterial activity evaluation for the highly concentrated palmitoleic acid (10 mg mL~(-1)) against Streptococcus agalactiae revealed the formation of a 12.10 mm-diameter inhibition zone and the minimum inhibitory concentration of 31.25 μg mL~(-1), indicating that palmitoleic acid is an effective antibacterial agent. This study is the first to report that palmitoleic acid derived from T. minus can antagonize S. agalactiae, which further broadens the potential application of Tribonema biomass in green aquaculture.
作者机构：
[He, Jiangling; Hu, Zhongze; Zhou, Jiaojiao; Cai, Jie; Zhu, Weijia; Chen, Lei; Dong, Qi; Zhang, Die; Liu, Xiaoqing] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[Ma, Tiezheng] Beijing Technol & Business Univ BTBU, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China.;[He, Jiangling; Zhou, Jiaojiao; Cai, Jie] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, 30023, Wuhan, Peoples R China.;[Din, Zia-ud] Univ Swabi, Dept Agr, Anbar 23561, Khyber Pakhtunk, Pakistan.
通讯机构：
[Jiangling He; Jie Cai] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, PR China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 30023, PR China
摘要：
Many efforts have been devoted to developing starch-based films as an alternative to non-degradable petroleum -based plastic food packaging. However, the application of starch-based films still faces serious challenges due to their water sensitivity and poor mechanical properties. In this work, a simple route was proposed to develop crosslinked starch nanofibrous films (C-SNFs) through electrospinning of starch solution, followed by cross -linking in glutaraldehyde (GTA) vapor. The effect of electrospinning parameters such as starch concentration (20-30 wt%), applied voltage (15-25 kV), and collecting distance (10-20 cm) on fiber morphology was studied. Compared with solution-cast starch films, electrospun starch nanofibrous films (SNFs) showed superior flexibility but poor mechanical strength and water sensitivity. After GTA crosslinking post-treatment, the tensile strength and water contact angle (WCA) of SNFs could be enhanced up to-9.65 MPa and-94.3 degrees, respectively. Results from this work could provide a facile route to fabricate starch-based film materials that showed a promising potential application in the food packaging field.
期刊：
Critical Reviews in Food Science and Nutrition,2022年63(31):10678-10690 ISSN：1040-8398
通讯作者：
Jingren He
作者机构：
[Shuai, Xiaoyan; Wang, Huiling; Zhang, Rui; Wu, Muci; He, Jingren] Wuhan Polytech Univ, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan, Peoples R China.;[Shuai, Xiaoyan; Wang, Huiling; Zhang, Rui; Wu, Muci; He, Jingren] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan, Peoples R China.;[Li, Yubao; Jiang, Sijia; Ye, Shuxin; Wu, Dong] China YunHong Holdings Co Ltd, Wuxue, Peoples R China.
通讯机构：
[Jingren He] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Wuhan Polytechnic University, Wuhan, P.R. China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, P.R. China
作者机构：
[Cheng, Shuiyuan; He, Yi; Dong, Xingxing; Wang, Zhangqian; Gao, Chao; Li, Mengjun; Wang, Yidan; Zhang, Sishang; Deng, Jieya; Duan, Yuhua] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan, Peoples R China.;[Wang, Zhangqian; Wang, Qin] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China.;[Yi, Tian] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Peoples R China.
通讯机构：
[Chao Gao; Zhangqian Wang] A;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Selenium is an essential microelement required for human health. The biotransformation of selenium nanoparticles has attracted increasing attention in recent years. However, little of the literature has investigated the comprehensive evaluation of the strains for practical application and the effect on the functional properties in the existence of Se. The present study showed the selenite reduction strain Bacillus subtilis T5 (up to 200 mM), which could produce high yields of selenium polysaccharides and selenium nanoparticles in an economical and feasible manner. Biosynthesized selenium nanoparticles by B. subtilis T5 were characterized systematically using UV-vis spectroscopy, FTIR, Zeta Potential, DLS, and SEM techniques. The biosynthesized SeNPs exhibited high stability with small particle sizes. B. subtilis T5 also possessed a tolerance to acidic pH and bile salts, high aggregation, negative hemolytic, and superior antioxidant activity, which showed excellent probiotic potential and can be recommended as a potential candidate for the selenium biopharmaceuticals industry. Remarkably, B. subtilis T5 showed that the activity of alpha-amylase was enhanced with selenite treatment to 8.12 U/mL, 2.72-fold more than the control. The genus Bacillus was first reported to produce both selenium polysaccharides with extremely high Se-content (2.302 g/kg) and significantly enhance the activity to promote alpha-amylase with selenium treatment. Overall, B. subtilis T5 showed potential as a bio-factory for the biosynthesized SeNPs and organ selenium (selenium polysaccharide), providing an appealing perspective for the biopharmaceutical industry.
通讯机构：
[Weiwei Zhang; Feng Xu] C;College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China<&wdkj&>College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China<&wdkj&>Spice Crops Research Institute, Yangtze University, Jingzhou 434025, Hubei, China
作者机构：
[Zhu, Zhenzhou] Wuhan Polytech Univ, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Wu, Sirong] Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Serich Agr Prod Proc, Wuhan 430023, Peoples R China.;[Wu, Sirong; Qi, Benkun; Luo, Jianquan] Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China.;[Wan, Yinhua; Luo, Jianquan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Wan, Yinhua] Chinese Acad Sci, Ganjiang Innovat Acad, Ganzhou, Jiangxi, Peoples R China.
通讯机构：
[Benkun Qi] S;State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
通讯机构：
[Shuiyuan Cheng] S;[Feng Xu] C;College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, National R&D Center for Se-rich Agricultural Products Processing Technology, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Nutrient imbalance (i.e., deficiency and toxicity) of microelements is an outstanding environmental issue that influences each aspect of ecosystems. Although the crucial roles of microelements in entire lifecycle of plants have been widely acknowledged, the effective control of microelements is still neglected due to the narrow safe margins. Selenium (Se) is an essential element for humans and animals. Although it is not believed to be indispensable for plants, many literatures have reported the significance of Se in terms of the uptake, accumulation, and detoxification of essential microelements in plants. However, most papers only concerned on the antagonistic effect of Se on metal elements in plants and ignored the underlying mechanisms. There is still a lack of systematic review articles to summarize the comprehensive knowledge on the connections between Se and microelements in plants. In this review, we conclude the bidirectional effects of Se on micronutrients in plants, including iron, zinc, copper, manganese, nickel, molybdenum, sodium, chlorine, and boron. The regulatory mechanisms of Se on these micronutrients are also analyzed. Moreover, we further emphasize the role of Se in alleviating element toxicity and adjusting the concentration of micronutrients in plants by altering the soil conditions (e.g., adsorption, pH, and organic matter), promoting microbial activity, participating in vital physiological and metabolic processes, generating element competition, stimulating metal chelation, organelle compartmentalization, and sequestration, improving the antioxidant defense system, and controlling related genes involved in transportation and tolerance. Based on the current understanding of the interaction between Se and these essential elements, future directions for research are suggested.
