期刊：
International Journal of Biological Macromolecules,2025年293:139340 ISSN：0141-8130
通讯作者：
Zhang, Rui;He, JR
作者机构：
[Chen, Ming; Pei, Xun; Yin, Jinjing; Zhang, Rui; Xiong, Sihui; Wu, Muci; He, Jingren] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Oliveira, Helder; Mateus, Nuno] Univ Porto, Fac Sci, Dept Chem & Biochem, REQUIMTE LAQV, P-4169007 Porto, Portugal.;[Ye, Shuxin] Yun Hong Grp Co Ltd, Wuhan 430206, Hubei, Peoples R China.;[Zhang, Rui; He, Jingren] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[He, Jingren; Zhang, Rui] Wuhan Polytech Univ, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.
通讯机构：
[He, JR ; Zhang, R] W;Wuhan Polytech Univ, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.
关键词：
Antioxidant activity;Antiproliferative capacity;Phenolics;Protein;Purple rice (Oryza sativa L.)
摘要：
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
期刊：
CURRENT MEDICINAL CHEMISTRY,2025年 ISSN：0929-8673
作者机构：
[He, Yi; Wang, Zhangqian; Gao, Chao; Wei, Shiya; Chen, Jixin] National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[Xu, Yang] Wuhan Polytechnic University School of Modern Industry for Selenium Science and Engineering Wuhan China;[Umer, Muhammad] Research Center of Forest Ecology, Institute for Forest Resources and Environment of Guizhou and Forestry College, Guizhou University, Guiyang, 550025, China;[Anwar, Naureen] Department of Biological Sciences, Faculty of Science and Technology, Virtual University of Pakistan, Punjab, 54000, Pakistan;[Liu, Wenbin] College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, 430023, China
摘要：
INTRODUCTION: G-quadruplexes (G4s) are non-classical high-level structures that are formed by DNA/RNA sequences and have been a promising target for developing antitumor drugs. However, it is still a challenge to find a ligand that binds to a particular G4 with selectivity. Telomeric multimeric G4s are more accessible for screening for specific ligands due to their higher-order structure compared with telomeric monomeric G4s. METHODS: In this study, the natural product berberine was found to exhibit a higher selectivity for telomeric multimeric G4 in comparison with other G4s. The mechanism of interaction between telomeric G4s and berberine was further investigated by fluorescence spectra measurements, job plot analysis, and UV titrations. We found that there are three binding sites for berberine on telomeric dimeric G-quadruplex Tel45, which are located at the 5' and 3' terminal G-quartet surfaces and the pocket between the two quadruplex units of Tel45. It was worth noting that the berberine preferred to interact within the interfacial cavity between two G4 units. RESULTS: Moreover, via dynamic light scattering (DLS) and native polyacrylamide gel electrophoresis (Native-PAGE) assays, it was found that the particle size of the telomeric multimeric G4s conformation was significantly increased by the addition of berberine. In contrast, the particle sizes of Tel21 did not change significantly after the addition of berberine. An immunofluorescence assay indicated that berberine induced the formation of endogenous telomeric G4 structures along with the related telomeric DNA damage response. CONCLUSION: This study provides a hypothetical basis for the development of natural products targeting telomeric G4 as antitumor drugs.
摘要：
Acid-catalyzed organosolv pretreatments using various acids and organic solvents have been widely studied for biomass fractionation. However, few studies have explored whether specific combinations of acids and solvents are necessary to achieve optimal enzymatic cellulose hydrolysis. In this study, organosolv pretreatments were performed on corn stover under mild conditions (120 °C, 2 h) using four biomass-derived solvents (ethylene glycol (EG), 1,4-butanediol (BDO), dimethyl isosorbide (DMI), and γ-valerolactone (GVL)) in an 80:20 solvent-to-water weight ratio, combined with four acids (HCl, H 2 SO 4 , AlCl 3 and p-toluenesulfonic acid (TsOH)) as catalysts (0.1 mol/L). The results showed specific interactions between the acid and solvent. HCl- and AlCl 3 -catalyzed GVL/H 2 O, HCl- and TsOH-catalyzed EG/H 2 O, and HCl-catalyzed DMI/H 2 O exhibited high pretreatment efficacy, achieving enzymatic glucose yields of approximately 80 % after 48 h of hydrolysis. The evaluation of solvent effects using Hansen Solubility Parameters (HSP) revealed no clear correlation with delignification, likely due to the influence of acidic catalysts and the formation of condensed lignin and pseudo-lignin, which may distort the delignification data. Additionally, the chemical composition and cellulose-related factors (accessibility, degree of polymerization and crystallinity) of pretreated biomass were analyzed and correlated with enzymatic glucose yield to evaluate their effects on biomass saccharification. In summary, this study underscores the specificity of acids in organosolv biomass pretreatment and cautions against relying solely on HSP theory for solvent selection when using acidic catalysts.
Acid-catalyzed organosolv pretreatments using various acids and organic solvents have been widely studied for biomass fractionation. However, few studies have explored whether specific combinations of acids and solvents are necessary to achieve optimal enzymatic cellulose hydrolysis. In this study, organosolv pretreatments were performed on corn stover under mild conditions (120 °C, 2 h) using four biomass-derived solvents (ethylene glycol (EG), 1,4-butanediol (BDO), dimethyl isosorbide (DMI), and γ-valerolactone (GVL)) in an 80:20 solvent-to-water weight ratio, combined with four acids (HCl, H 2 SO 4 , AlCl 3 and p-toluenesulfonic acid (TsOH)) as catalysts (0.1 mol/L). The results showed specific interactions between the acid and solvent. HCl- and AlCl 3 -catalyzed GVL/H 2 O, HCl- and TsOH-catalyzed EG/H 2 O, and HCl-catalyzed DMI/H 2 O exhibited high pretreatment efficacy, achieving enzymatic glucose yields of approximately 80 % after 48 h of hydrolysis. The evaluation of solvent effects using Hansen Solubility Parameters (HSP) revealed no clear correlation with delignification, likely due to the influence of acidic catalysts and the formation of condensed lignin and pseudo-lignin, which may distort the delignification data. Additionally, the chemical composition and cellulose-related factors (accessibility, degree of polymerization and crystallinity) of pretreated biomass were analyzed and correlated with enzymatic glucose yield to evaluate their effects on biomass saccharification. In summary, this study underscores the specificity of acids in organosolv biomass pretreatment and cautions against relying solely on HSP theory for solvent selection when using acidic catalysts.
摘要：
Chitosan (CTS) exhibits notable moisturizing and film-forming capabilities, whereas nano selenium (SeNPs) demonstrates antioxidant and antibacterial properties. In the present study, a CTS/Se film-forming agent was prepared by dissolving CTS, glycerol, and SeNPs in 1% (v/v) glacial acetic acid through heating and ultrasonic treatment. The results revealed that SeNPs fused with CTS via intermolecular forces, thereby enhancing the tensile strength of the CTS film. The water contact angle of the CTS film increased with an increase in SeNPs concentration. The thermal stability and water retention properties of CTS/Se were superior than CTS. In a storage experiment, the content of soluble solids in tomatoes coated with CTS/Se was higher than that in tomatoes coated with CTS and the control. The CTS/Se coating inhibited gas exchange both inside and outside the film, leading to increased activities of antioxidant enzymes. This reduced the oxidation of vitamin C in the tomatoes and decreased the content of malondialdehyde. The CTS/Se film inhibited the growth of bacteria on the tomato surface and mitigated the decline of aldehyde, alcohol and ketone aroma compounds. Consequently, the CTS/Se coating alleviated the softening, aging and rotting of tomatoes. Feeding experiments conducted on mice verified the food safety of the CTS/Se.
Chitosan (CTS) exhibits notable moisturizing and film-forming capabilities, whereas nano selenium (SeNPs) demonstrates antioxidant and antibacterial properties. In the present study, a CTS/Se film-forming agent was prepared by dissolving CTS, glycerol, and SeNPs in 1% (v/v) glacial acetic acid through heating and ultrasonic treatment. The results revealed that SeNPs fused with CTS via intermolecular forces, thereby enhancing the tensile strength of the CTS film. The water contact angle of the CTS film increased with an increase in SeNPs concentration. The thermal stability and water retention properties of CTS/Se were superior than CTS. In a storage experiment, the content of soluble solids in tomatoes coated with CTS/Se was higher than that in tomatoes coated with CTS and the control. The CTS/Se coating inhibited gas exchange both inside and outside the film, leading to increased activities of antioxidant enzymes. This reduced the oxidation of vitamin C in the tomatoes and decreased the content of malondialdehyde. The CTS/Se film inhibited the growth of bacteria on the tomato surface and mitigated the decline of aldehyde, alcohol and ketone aroma compounds. Consequently, the CTS/Se coating alleviated the softening, aging and rotting of tomatoes. Feeding experiments conducted on mice verified the food safety of the CTS/Se.
摘要：
Glycyrrhizae Radix et Rhizoma (Gancao) is a functional food whose quality varies significantly owing to the genetic and geographical factors, and is often used by unscrupulous merchants as a substitute for profit. This study developed a rapid identification method for Gancao that determined the species, geographical origin, and main quality marker using a six-channel visual array sensor. This sensor detected the color changes resulting from the competitive coordination with metal ions and color-changing organic dyes. The combination of the array sensor with a random forest (RF) algorithm achieved 100% accuracy in identifying Gancao. Additionally, the smartphone app enabled the rapid and intelligent identification of Gancao within 1 min. The identification accuracy was 91.43%, and the prediction error of two quality markers was less than 13%. This research provides a foundation for rapid and reliable assessment of Gancao quality, enabling the industry to combat fraudulent practices effectively.
Glycyrrhizae Radix et Rhizoma (Gancao) is a functional food whose quality varies significantly owing to the genetic and geographical factors, and is often used by unscrupulous merchants as a substitute for profit. This study developed a rapid identification method for Gancao that determined the species, geographical origin, and main quality marker using a six-channel visual array sensor. This sensor detected the color changes resulting from the competitive coordination with metal ions and color-changing organic dyes. The combination of the array sensor with a random forest (RF) algorithm achieved 100% accuracy in identifying Gancao. Additionally, the smartphone app enabled the rapid and intelligent identification of Gancao within 1 min. The identification accuracy was 91.43%, and the prediction error of two quality markers was less than 13%. This research provides a foundation for rapid and reliable assessment of Gancao quality, enabling the industry to combat fraudulent practices effectively.
期刊：
International Journal of Biological Macromolecules,2025年287:138595 ISSN：0141-8130
通讯作者：
Wu, Q
作者机构：
[Ma, Shuzhen; Wang, Jingyi; Zhang, Xue; Wu, Qian; Tao, Sihong] Hubei Univ Technol, Cooperat Innovat Ctr Ind Fermentat, Minist Educ & Hubei Prov, Wuhan 430068, Peoples R China.;[Ma, Shuzhen; Wang, Jingyi; Zhang, Xue; Wu, Qian; Tao, Sihong] Hubei Univ Technol, Sch Life & Hlth Sci, Wuhan 430068, Peoples R China.;[Wu, Muci] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.
通讯机构：
[Wu, Q ] H;Hubei Univ Technol, Sch Life & Hlth Sci, Wuhan 430068, Peoples R China.
关键词：
Casein;Protein-polysaccharide complex coacervates;Low frequency-static magnetic field treatment
摘要：
To evaluate the combination effects of highland barley polysaccharides (HBP) and low-frequency static magnetic field (LF-SMF) treatment on the structure and properties of acid-induced casein (CS) coacervates, this study conducted a comprehensive investigation at various stages- before, during, and after coacervation-for the first time. Compared with native CS, adding HBP caused CS to denature owing to hydrophobic and electrostatic interactions, and LF-SMF treatment further promoted these changes. During the acidification (pH 7.0–2.0) and coacervation processes, the integration of LF-SMF treatment with the addition of HBP enhanced the rate and extent of casein (CS) aggregation and crosslinking, attributable to alterations in the ζ-potential of CS. Among the formed coacervates, the yield and particle size of CS/HBP complex coacervates after being treated with LF-SMF (M-CS/HBP) increased from 67.9 % to 78.4 % and from 803.12 nm to 1253.43 nm, respectively. Additionally, M-CS/HBP demonstrated improved viscoelasticity and a more uniform, compact microstructure with denser packing. The alterations observed in CS-based coacervates were due to non-covalent interactions between CS and HBP, further promoted by LF-SMF treatment, leading to the unfolding and disordering of protein secondary structure. Consequently, M-CS/HBP complex coacervates demonstrated superior encapsulation efficiency for L. plantarum and provided enhanced protection for probiotics under adverse environments.
To evaluate the combination effects of highland barley polysaccharides (HBP) and low-frequency static magnetic field (LF-SMF) treatment on the structure and properties of acid-induced casein (CS) coacervates, this study conducted a comprehensive investigation at various stages- before, during, and after coacervation-for the first time. Compared with native CS, adding HBP caused CS to denature owing to hydrophobic and electrostatic interactions, and LF-SMF treatment further promoted these changes. During the acidification (pH 7.0–2.0) and coacervation processes, the integration of LF-SMF treatment with the addition of HBP enhanced the rate and extent of casein (CS) aggregation and crosslinking, attributable to alterations in the ζ-potential of CS. Among the formed coacervates, the yield and particle size of CS/HBP complex coacervates after being treated with LF-SMF (M-CS/HBP) increased from 67.9 % to 78.4 % and from 803.12 nm to 1253.43 nm, respectively. Additionally, M-CS/HBP demonstrated improved viscoelasticity and a more uniform, compact microstructure with denser packing. The alterations observed in CS-based coacervates were due to non-covalent interactions between CS and HBP, further promoted by LF-SMF treatment, leading to the unfolding and disordering of protein secondary structure. Consequently, M-CS/HBP complex coacervates demonstrated superior encapsulation efficiency for L. plantarum and provided enhanced protection for probiotics under adverse environments.
摘要：
In this study, the physicochemical properties, in vitro fermentation, and interaction with Bacteroides ovatus of a purified fraction of polysaccharides from the root of Brassica rapa (BRP2–2) were investigated. It was demonstrated with an in vitro anaerobic fermentation model that BRP2–2 significantly increased ( p < 0.05) the relative abundance of Bacteroides spp. After 24 h incubation of BRP2–2 with Bacteroides ovatus alone, 10.24 ± 0.69, 8.76 ± 0.48 and 3.37 ± 0.26 mM of acetate, propionate and isovalerate were produced, respectively. Moreover, transcriptome analysis of B. ovatus showed that 143 genes were up-regulated by BRP2–2, including five discrete polysaccharide utilization loci and two carbohydrate-active enzyme clusters. Based on the annotation of carbohydrate enzyme function, we speculate that BRP2–2 is a pectic polysaccharide containing homogalacturonan, rhamnogalacturonan I and rhamnogalacturonan II domains. These results suggested that BRP2–2 was degraded by B. ovatus , which produced metabolites with beneficial effects on host health.
In this study, the physicochemical properties, in vitro fermentation, and interaction with Bacteroides ovatus of a purified fraction of polysaccharides from the root of Brassica rapa (BRP2–2) were investigated. It was demonstrated with an in vitro anaerobic fermentation model that BRP2–2 significantly increased ( p < 0.05) the relative abundance of Bacteroides spp. After 24 h incubation of BRP2–2 with Bacteroides ovatus alone, 10.24 ± 0.69, 8.76 ± 0.48 and 3.37 ± 0.26 mM of acetate, propionate and isovalerate were produced, respectively. Moreover, transcriptome analysis of B. ovatus showed that 143 genes were up-regulated by BRP2–2, including five discrete polysaccharide utilization loci and two carbohydrate-active enzyme clusters. Based on the annotation of carbohydrate enzyme function, we speculate that BRP2–2 is a pectic polysaccharide containing homogalacturonan, rhamnogalacturonan I and rhamnogalacturonan II domains. These results suggested that BRP2–2 was degraded by B. ovatus , which produced metabolites with beneficial effects on host health.
摘要：
The mortality rate of tumor is still very high till now. Circulating tumor cells (CTCs) are the major culprit of high cancer mortality. To improve survival rate of cancer patients, real-time monitoring and quantitative detection of CTCs are of indescribable value. However, due to the extremely small content and heterogeneous characteristics of CTCs, to accurately detect CTCs is still a tremendous challenge in clinical trials. Herein, the photoelectrochemical aptasensing and fluorescence imaging were co-joint to detect MCF-7 cells in whole blood via an inertial separation microfluidic chip. A portable inertial microfluidic chip with a height of 150 μm was designed to separate MCF-7 cells from whole blood samples. Niblue-C6-IMC was used to label and image MCF-7 cells through specifically reacting with COX-2 enzyme from the over-expression on the cellular surface. Subsequently, MCF-7 cells were detected with a Bi 2 O 2 S nanoflower based photoelectrochemical sensing system. Parameters including the channel height and length, flow rate, focusing position, fluorescence probe concentration, and flow length of the microfluidic chip were optimized. The separation efficiency and purity of MCF-7 cells were 85 % and 80 %, respectively. For 1 mL of blood sample (100 cells mL −1 ), 85 cells and 90 cells could be calculated based on the signals from fluorescence imaging and photoelectrochemical response, respectively. Nevertheless, for assaying 1 mL of blood samples containing 50 MCF-7 cells, 40 cells and 39 cells were obtained from fluorescence imaging and photoelectrochemical responses, respectively.
The mortality rate of tumor is still very high till now. Circulating tumor cells (CTCs) are the major culprit of high cancer mortality. To improve survival rate of cancer patients, real-time monitoring and quantitative detection of CTCs are of indescribable value. However, due to the extremely small content and heterogeneous characteristics of CTCs, to accurately detect CTCs is still a tremendous challenge in clinical trials. Herein, the photoelectrochemical aptasensing and fluorescence imaging were co-joint to detect MCF-7 cells in whole blood via an inertial separation microfluidic chip. A portable inertial microfluidic chip with a height of 150 μm was designed to separate MCF-7 cells from whole blood samples. Niblue-C6-IMC was used to label and image MCF-7 cells through specifically reacting with COX-2 enzyme from the over-expression on the cellular surface. Subsequently, MCF-7 cells were detected with a Bi 2 O 2 S nanoflower based photoelectrochemical sensing system. Parameters including the channel height and length, flow rate, focusing position, fluorescence probe concentration, and flow length of the microfluidic chip were optimized. The separation efficiency and purity of MCF-7 cells were 85 % and 80 %, respectively. For 1 mL of blood sample (100 cells mL −1 ), 85 cells and 90 cells could be calculated based on the signals from fluorescence imaging and photoelectrochemical response, respectively. Nevertheless, for assaying 1 mL of blood samples containing 50 MCF-7 cells, 40 cells and 39 cells were obtained from fluorescence imaging and photoelectrochemical responses, respectively.
作者机构：
[Xie, Fang; Luo, Yajie; He, Zhijun; Chen, Qianqian] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China;[Qin, Zhiqin] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China;[Cai, Jie] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China
通讯机构：
[Jie Cai] S;School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China
摘要：
This study introduces a starch-based composite film integrated with quaternary ammonium chitosan (QCS) and Lycium ruthenicum anthocyanins (LRA) via a facile casting method, designed for intelligent food packaging. The influences of varying concentrations of QCS and/or LRA on water sensitivity, mechanical attributes, UV transmittance, antioxidant capacity, antibacterial performance, and pH-responsive characteristics were meticulously examined. The optimized film demonstrated a tensile strength of ∼0.68 MPa, a contact angle of ∼123.69°, and ABTS radical scavenging efficiency exceeding 80 %. The film exhibited pH-responsive color changes from pink to green across a pH range of 2–12, alongside excellent UV-blocking and antibacterial properties. Shrimp preservation experiments revealed a 16-h shelf-life extension, coupled with real-time freshness monitoring. These findings put forward an exceedingly promising approach to the promotion of starch-based films boasting diverse functionalities in the realm of intelligent food packaging, signifying a stride in this domain.
This study introduces a starch-based composite film integrated with quaternary ammonium chitosan (QCS) and Lycium ruthenicum anthocyanins (LRA) via a facile casting method, designed for intelligent food packaging. The influences of varying concentrations of QCS and/or LRA on water sensitivity, mechanical attributes, UV transmittance, antioxidant capacity, antibacterial performance, and pH-responsive characteristics were meticulously examined. The optimized film demonstrated a tensile strength of ∼0.68 MPa, a contact angle of ∼123.69°, and ABTS radical scavenging efficiency exceeding 80 %. The film exhibited pH-responsive color changes from pink to green across a pH range of 2–12, alongside excellent UV-blocking and antibacterial properties. Shrimp preservation experiments revealed a 16-h shelf-life extension, coupled with real-time freshness monitoring. These findings put forward an exceedingly promising approach to the promotion of starch-based films boasting diverse functionalities in the realm of intelligent food packaging, signifying a stride in this domain.
摘要：
This study explores selenium enrichment of milk through microbial transformation, utilizing Geotrichum candidum LG-8, isolated from traditional dairy products. A combination of electronic tongue, electronic nose, HPLC-ICP-MS, and UHPLC-QTOF-MS were used to evaluate milk quality, organic selenium types, and various components. The milk retained a weakly acidic pH post-transformation, with a flavor profile rich in sour, sweet, and umami tastes. Odor analysis revealed predominant contributions from nitrogen oxides, aromatic compounds, ketones, and organic sulfides. Selenium concentrations increased with higher initial selenium levels, with 50 μg/mL yielding concentrations of MeSeCys, SeCys2, Se(IV), SeMet, and Se(VI) at 5.15, 20.80, 1.41, 75.31 and 0.44 μg/L respectively. Significant changes were observed in the milk’s amino acid profile, with metabolite identification and metabolic pathways mapping(including KEGG). The findings highlight the potential of Geotrichum candidum LG-8 for generating functional organic selenium-enriched milk, offering promising nutritional benefits in dairy production.
This study explores selenium enrichment of milk through microbial transformation, utilizing Geotrichum candidum LG-8, isolated from traditional dairy products. A combination of electronic tongue, electronic nose, HPLC-ICP-MS, and UHPLC-QTOF-MS were used to evaluate milk quality, organic selenium types, and various components. The milk retained a weakly acidic pH post-transformation, with a flavor profile rich in sour, sweet, and umami tastes. Odor analysis revealed predominant contributions from nitrogen oxides, aromatic compounds, ketones, and organic sulfides. Selenium concentrations increased with higher initial selenium levels, with 50 μg/mL yielding concentrations of MeSeCys, SeCys2, Se(IV), SeMet, and Se(VI) at 5.15, 20.80, 1.41, 75.31 and 0.44 μg/L respectively. Significant changes were observed in the milk’s amino acid profile, with metabolite identification and metabolic pathways mapping(including KEGG). The findings highlight the potential of Geotrichum candidum LG-8 for generating functional organic selenium-enriched milk, offering promising nutritional benefits in dairy production.
作者机构：
[He, Zhijun; Luo, Changsheng; Zhou, Jiaojiao; Cai, Jie; Chen, Qianqian; Xie, Fang] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[He, ZJ; Cai, J ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
3D printing;Curcumin;Food preservation;Photodynamics;Starch
摘要：
Here, a facile self-assembly strategy was used to fabricate octenyl succinic anhydride starch (OSAS) nano micelles for encapsulation of K-carrageenan comprising curcumin (Cur) (KC/Cur-OSAS). KC/Cur-OSAS was used as a multipurpose edible food packaging coating on grapes. The characteristics, storage stability, photoactivated antibacterial properties and antimicrobial mechanisms of KC/Cur-OSAS were investigated. The experimental results demonstrated the potential photoactivated antibacterial performance of KC/Cur-OSAS gel coating, with an antibacterial efficacy of more than of 99 % against S. aureus and E. coli. Moreover, the coating showed good biocompatibility and 3D printing potential, and could reduce dewatering, prevent nutrient degradation, inhibit bacterial proliferation, increase nutritional content, maintain food quality, and extend the shelf life of food by more than 3 days, when conserved at a temperature of 25 °C. Hence, the light-driven KC/Cur-OSAS coatings prepared in this has great potential for improving nutrient loading and extending the shelf life of perishable food and can be used for developing food packaging and preservation materials.
Here, a facile self-assembly strategy was used to fabricate octenyl succinic anhydride starch (OSAS) nano micelles for encapsulation of K-carrageenan comprising curcumin (Cur) (KC/Cur-OSAS). KC/Cur-OSAS was used as a multipurpose edible food packaging coating on grapes. The characteristics, storage stability, photoactivated antibacterial properties and antimicrobial mechanisms of KC/Cur-OSAS were investigated. The experimental results demonstrated the potential photoactivated antibacterial performance of KC/Cur-OSAS gel coating, with an antibacterial efficacy of more than of 99 % against S. aureus and E. coli. Moreover, the coating showed good biocompatibility and 3D printing potential, and could reduce dewatering, prevent nutrient degradation, inhibit bacterial proliferation, increase nutritional content, maintain food quality, and extend the shelf life of food by more than 3 days, when conserved at a temperature of 25 °C. Hence, the light-driven KC/Cur-OSAS coatings prepared in this has great potential for improving nutrient loading and extending the shelf life of perishable food and can be used for developing food packaging and preservation materials.
摘要：
Sausage is favored due to its rich nutrition and good taste, but usually contains high sodium concentration. Additionally, physicochemical changes happening during the cooking process of sausage resulted in water loss and poor flavor. Plant proteins are important ingredients in meat alternatives. This study explored the effect of plant proteins on the physical and flavor properties of reduced-sodium pork sausages. The results showed that soy protein isolate had the highest gel strength, water holding capacity (WHC) and oil holding capacity (OHC), pea protein had higher gel strength, WHC and OHC than peanut protein. Besides, the emulsion activity of soy protein isolate and pea protein was higher than that of peanut protein. KCl substitution minimally affected the color of soy protein isolate sausage and WHC of pea protein sausage. LF-NMR results showed KCl didn't change water distribution of sausages. Electronic tongue analysis revealed enhanced umami and reduced sourness with increased KCl in pea protein sausage. Furthermore, Soy protein isolate sausage with 20% KCl substitution and pea protein sausage with 40% KCl substitution had the highest sensory scores. Overall, soy protein isolate and pea protein are promising ingredients with 20% and 30%–40% KCl substitution in sausage, respectively.
Sausage is favored due to its rich nutrition and good taste, but usually contains high sodium concentration. Additionally, physicochemical changes happening during the cooking process of sausage resulted in water loss and poor flavor. Plant proteins are important ingredients in meat alternatives. This study explored the effect of plant proteins on the physical and flavor properties of reduced-sodium pork sausages. The results showed that soy protein isolate had the highest gel strength, water holding capacity (WHC) and oil holding capacity (OHC), pea protein had higher gel strength, WHC and OHC than peanut protein. Besides, the emulsion activity of soy protein isolate and pea protein was higher than that of peanut protein. KCl substitution minimally affected the color of soy protein isolate sausage and WHC of pea protein sausage. LF-NMR results showed KCl didn't change water distribution of sausages. Electronic tongue analysis revealed enhanced umami and reduced sourness with increased KCl in pea protein sausage. Furthermore, Soy protein isolate sausage with 20% KCl substitution and pea protein sausage with 40% KCl substitution had the highest sensory scores. Overall, soy protein isolate and pea protein are promising ingredients with 20% and 30%–40% KCl substitution in sausage, respectively.
摘要：
Selenium (Se) is an indispensable trace element for humans and other animals. Various studies have demonstrated the beneficial effects of Se on plants, including the promotion of growth, accumulation of secondary metabolites, and enhancement of antioxidant capacity, thereby improving plant stress resistance. Consequently, Se biofortification has emerged as an effective strategy to elevate Se content and nutritional quality in plants, attracting widespread attention. The mechanism of selenium (Se) at the plant secondary metabolic level has not yet been fully elucidated, and it remains an unanswered question as to how selenium affects plant secondary metabolic pathways and how these metabolic pathways respond to selenium biofortification. Although it has been shown that selenium can affect the antioxidant system and defense mechanisms in plants, detailed mechanisms of selenium’s action on plant secondary metabolic pathways, including its effects on specific metabolic enzymes and regulatory genes, still need to be revealed by further in-depth studies. The present study aims to elucidate the mechanisms of Se absorption, transport, and metabolism in plants under Se-rich conditions and to investigate the impact of various Se biofortification methods on the content of plant secondary metabolites. By integrating existing research progress, this paper will delve into the potential molecular regulatory mechanisms of Se on plant secondary metabolism, aiming to unravel the interplay between Se and plant secondary metabolism. This study provides a novel perspective and direction for future research on plant secondary metabolism and the biological utilization of Se.
摘要：
Bioactive peptides represent a promising therapeutic approach for Alzheimer's disease (AD) by maintaining cholinergic system homeostasis through the inhibition of acetylcholinesterase (AChE) activity. This study focused on extracting AChE inhibitory peptides from yellowfin tuna pancreas using moderate ultrasound-assisted enzymatic hydrolysis (MUE). Firstly, papain and MUE stood out from five enzymes and four enzymatic hydrolysis methods, respectively, by comparing the degree of hydrolysis and AChE inhibitory activity of different pancreatic protein hydrolysates. Subsequently, the optimal MUE conditions were obtained by single-factor, Plackett-Burman, and response surface methodologies. The pancreatic protein hydrolysate prepared under optimal MUE conditions was then purified by ultrafiltration followed by RP-HPLC, from which a novel AChE inhibitory peptide (LLDF) was identified by LC-MS/MS and virtual screening. LLDF effectively inhibited AChE activity by a competitive inhibition mechanism, with an IC(50) of 18.44 ± 0.24 μM. Molecular docking and molecular dynamic simulation revealed that LLDF bound robustly to the active site of AChE via hydrogen bonds. These findings provided a theoretical basis for the valuable use of yellowfin tuna pancreas and introduced a new viewpoint on the potential therapeutic advantages of AChE inhibitory peptides for future AD treatment.
摘要：
Resistant starch (RS) refers to starch varieties that resist digestion by human digestive enzymes. Owing to its distinctive physicochemical attributes and functional capabilities, RS has gained a wide range of applications as a dietary fiber and prebiotic. In terms of structure and functions, RS can be categorized into five distinct types: RS1 through RS5. These types offer dietary benefits, contributing to improved colonic health, the modulation of microbial communities, the reduction in gallstone formation, the enhancement of mineral absorption, and alterations in fat oxidation potential. From a technical standpoint, RS can be manufactured through an array of physical, enzymatic, and chemical modifications. This paper presents a comprehensive review of the existing literature, summarizing the classification, structural features, raw material origins, preparation methodologies, and functionalities of RS. Furthermore, new production technologies and applications of RS, such as 3D printing, provide valuable insights.
关键词：
Carotenoids;Microbial count;Minimally processed vegetables;Polyphenol oxidase and peroxidase;Sugars
摘要：
Supercritical carbon dioxide (SCCD) has been studied for its effectiveness in preserving vegetables, but most research has focused on overall changes in the total phenolic, carotenoid and sugar contents. However, the detailed changes in individual nutrients during storage, as well as their relationship, remain largely unexplored. Herein, in this work, high-performance liquid chromatography (HPLC) was used to analyze the variations in individual carotenoids and sugars in SCCD-treated carrots and pumpkins during 21 days of storage. The results demonstrated that SCCD effectively inhibited microbial growth, and maintained the low levels of polyphenol oxidase (PPO) and peroxidase (POD) activity during storage. SCCD also deferred the degradation of carotenoid profile and phenolic compounds. Additionally, SCCD accelerated the hydrolysis of sucrose content while postponing the reduction of glucose and fructose. In summary, the SCCD technique could delay the quality deterioration of fresh-cut vegetables, preserving them with minimal quality changes.
Supercritical carbon dioxide (SCCD) has been studied for its effectiveness in preserving vegetables, but most research has focused on overall changes in the total phenolic, carotenoid and sugar contents. However, the detailed changes in individual nutrients during storage, as well as their relationship, remain largely unexplored. Herein, in this work, high-performance liquid chromatography (HPLC) was used to analyze the variations in individual carotenoids and sugars in SCCD-treated carrots and pumpkins during 21 days of storage. The results demonstrated that SCCD effectively inhibited microbial growth, and maintained the low levels of polyphenol oxidase (PPO) and peroxidase (POD) activity during storage. SCCD also deferred the degradation of carotenoid profile and phenolic compounds. Additionally, SCCD accelerated the hydrolysis of sucrose content while postponing the reduction of glucose and fructose. In summary, the SCCD technique could delay the quality deterioration of fresh-cut vegetables, preserving them with minimal quality changes.
摘要：
Bee pollen, a natural honeybee product, is hailed as a treasure trove of human nutrition. Among the nourishing substances of bee pollen, the constituents with a low molecular weight (such as phenolic acids and flavonoid glycosides) have been extensively studied in the past decades, whereas the polysaccharides with a relatively high molecular weight have received much less attention. To deepen our understanding of bee pollen polysaccharides, this review summarizes the published findings related to their preparation technologies, structural characteristics and biological activities. Among the preparation technologies, ultrasonic-assisted extraction is currently the most effective technology for the recovery of polysaccharides from bee pollen, because ultrasound can crack the pollen exine into fragments and facilitate the release of polysaccharides present in the pollen intine. The preliminary structures, including the molecular weight and monosaccharide composition, of bee pollen polysaccharides have been widely reported, but their fine structures have not fully elucidated. Moreover, bee pollen polysaccharides have antioxidant, immunomodulatory, and antitumor activities, exhibiting potential application in functional foods. Furthermore, bee pollen polysaccharides can modulate the composition of gut microbiota and promote the production of short-chain fatty acids. It is expected that this review can provide inspiration for the development and utilization of bee pollen polysaccharides.
Bee pollen, a natural honeybee product, is hailed as a treasure trove of human nutrition. Among the nourishing substances of bee pollen, the constituents with a low molecular weight (such as phenolic acids and flavonoid glycosides) have been extensively studied in the past decades, whereas the polysaccharides with a relatively high molecular weight have received much less attention. To deepen our understanding of bee pollen polysaccharides, this review summarizes the published findings related to their preparation technologies, structural characteristics and biological activities. Among the preparation technologies, ultrasonic-assisted extraction is currently the most effective technology for the recovery of polysaccharides from bee pollen, because ultrasound can crack the pollen exine into fragments and facilitate the release of polysaccharides present in the pollen intine. The preliminary structures, including the molecular weight and monosaccharide composition, of bee pollen polysaccharides have been widely reported, but their fine structures have not fully elucidated. Moreover, bee pollen polysaccharides have antioxidant, immunomodulatory, and antitumor activities, exhibiting potential application in functional foods. Furthermore, bee pollen polysaccharides can modulate the composition of gut microbiota and promote the production of short-chain fatty acids. It is expected that this review can provide inspiration for the development and utilization of bee pollen polysaccharides.
作者：
Chao Gao;Shiya Wei;Yang Xu;Hany I. Mohamed;Wenbin Liu;...
期刊：
Pest Management Science,2025年 ISSN：1526-498X
通讯作者：
Wenqiang Wu<&wdkj&>Yi He
作者机构：
[Chao Gao; Shiya Wei; Yang Xu; Zhangqian Wang; Yi He] National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, China;[Wenqiang Wu] State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, China;[Wenbin Liu] College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China;[Hany I. Mohamed] State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China;[Mo Wang] College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
通讯机构：
[Wenqiang Wu] S;[Yi He] N;National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, China<&wdkj&>State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, China
通讯机构：
[Rao, S ; Liu, XM] W;Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
White tea;Selenium;Mineral;Amino acid;Correlation analysis
摘要：
Tea, second only to water in consumption, is rich in nutrients. Selenium, a crucial trace element, has unclear relationships with other substances in tea leaves. This study analyzed five white teas, measuring selenium, five minerals, and various chemical components. The results showed that there were significant differences in the elemental and chemical characteristics among these tea. Correlation analysis showed that low-level selenium might have a synergistic effect with iron, magnesium and potassium, while the correlations between other elements in tea leaves, total proteins, and total amino acids with selenium were not significant. In addition, the contents of flavonoids and polyphenols were significantly negatively correlated with the selenium content. These results indicated that selenium has a significant impact on the accumulation of minerals, flavonoids, and polyphenols in tea leaves. This study disclosed white tea's elemental and chemical basis, supplying a theoretical basis for quality evaluation, crucial for the tea industry.
Tea, second only to water in consumption, is rich in nutrients. Selenium, a crucial trace element, has unclear relationships with other substances in tea leaves. This study analyzed five white teas, measuring selenium, five minerals, and various chemical components. The results showed that there were significant differences in the elemental and chemical characteristics among these tea. Correlation analysis showed that low-level selenium might have a synergistic effect with iron, magnesium and potassium, while the correlations between other elements in tea leaves, total proteins, and total amino acids with selenium were not significant. In addition, the contents of flavonoids and polyphenols were significantly negatively correlated with the selenium content. These results indicated that selenium has a significant impact on the accumulation of minerals, flavonoids, and polyphenols in tea leaves. This study disclosed white tea's elemental and chemical basis, supplying a theoretical basis for quality evaluation, crucial for the tea industry.
作者机构：
School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, PR China;National R&D Center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan, 430023, PR China;[Chen, Xu; Xiang, Hongxia; Liang, Yong; He, Jiangling; Chen, Rongyu; Zhu, Zhenzhou; Li, Shuyi; Chen, Xiang; Cheng, Shuiyuan] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, PR China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan, 430023, PR China
通讯机构：
[Xu Chen] S;School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, PR China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan, 430023, PR China
摘要：
Highly stable and multifunctional intelligent films were fabricated using a combination of grape skin anthocyanin, polyvinyl alcohol, chitosan and selenopeptide, and the influence of selenopeptide concentration on films' properties and their effectiveness in strawberry preservation and freshness monitoring was investigated. The results showed that the incorporated selenopeptide could interact with polyvinyl alcohol and grape skin anthocyanin via hydrogen bonding, improving the hydrophobic, UV-blocking, and mechanical characteristics of the films. In particular, the introduction of 0.4% selenopeptide into the film notably boosted its tensile strength from 11.91 MPa to 26.91 MPa. More importantly, the films incorporated with selenopeptide showed decent antioxidant and antibacterial properties, along with high storage stability and thermal stability. Adding 0.4% selenopeptide to the film can increase the shelf life of strawberries at 25 °C by 3 days, and a distinct color change showing fair good linear relationship with the freshness indexes of the strawberry (hardness and weight loss rate) was observed. Overall, anthocyanin-infused films combined with selenopeptide have demonstrated promising results in preserving food and monitoring freshness, opening up new opportunities for preparing stable, smart and active films.
Highly stable and multifunctional intelligent films were fabricated using a combination of grape skin anthocyanin, polyvinyl alcohol, chitosan and selenopeptide, and the influence of selenopeptide concentration on films' properties and their effectiveness in strawberry preservation and freshness monitoring was investigated. The results showed that the incorporated selenopeptide could interact with polyvinyl alcohol and grape skin anthocyanin via hydrogen bonding, improving the hydrophobic, UV-blocking, and mechanical characteristics of the films. In particular, the introduction of 0.4% selenopeptide into the film notably boosted its tensile strength from 11.91 MPa to 26.91 MPa. More importantly, the films incorporated with selenopeptide showed decent antioxidant and antibacterial properties, along with high storage stability and thermal stability. Adding 0.4% selenopeptide to the film can increase the shelf life of strawberries at 25 °C by 3 days, and a distinct color change showing fair good linear relationship with the freshness indexes of the strawberry (hardness and weight loss rate) was observed. Overall, anthocyanin-infused films combined with selenopeptide have demonstrated promising results in preserving food and monitoring freshness, opening up new opportunities for preparing stable, smart and active films.
