作者机构：
[Cai, Jie; Xie, F; Xie, Fang; Zhang, Die] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Zhang, Die] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J; Xie, F ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
摘要：
The work aimed to explore the effect of supercritical carbon dioxide (SCCD) treatment on the enzyme activity, color, carotenoid, sugar and phenolic profile, and antioxidant activity of fresh-cut pumpkins. The polyphenol oxidase (PPO) and peroxidase (POD) activities of SCCD-treated pumpkin cubes were reduced by 21% and 18%, respectively. The lightness value of SCCD-treated samples declined compared to the untreated samples, whereas the redness and yellowness values in treated samples were enhanced. The lutein, alpha-carotene, beta-carotene and total carotenoids, glucose, sorbitol, and other polysaccharides in the SCCD-treated samples showed a fluctuation trend. Similarly, the treated sample's total phenolic contents (TPC), ABTS (2,2 '- azino-bis(3ethylbenzothiazoline-6-sulfonic acid, DPPH (2,2-diphenyl-1-picrylhydrazyl), and superoxide radical scavenging activities were increased and then reduced. An enhancement was noted in the coumaryloquinic acid I and II, caffeic acid glucoside, 4-hydroxybenzoic acid, and p-coumaric acid of SCCD-treated samples. The work demonstrated that the SCCD technique at moderate conditions could obtain higher retention of nutritional composition in fresh -cut pumpkins.
作者机构：
These authors contributed equally to this work.;The author, Xin Cong, is also employed by Enshi Se-Run Material Engineering Technology Co., Ltd., Enshi 445000, China.;[Cheng, Shuiyuan] National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Wang, Hui; Liu, Mingkang; Xu, Xiao; Liu, Yulan; Xu, Xianfeng; Yan, Mengke; Song, Fulong; Wang, Dan] Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China;Author to whom correspondence should be addressed.
通讯机构：
[Huiling Zhu] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Oxidative stress occurs in the process of egg storage. Antioxidants as feed additives can enhance egg quality and extend the shelf life of eggs. Selenium-enriched Cardamine violifolia (SEC) has strongly antioxidant properties. The objective of this study was to assess the effects of dietary supplementation with SEC on egg quality and the yolk antioxidant capacity of eggs stored at 4 °C and 25 °C. Four hundred fifty 65-week-old, Roman hens that were similar in laying rate (90.79 ± 1.69%) and body weight (2.19 ± 0.23 kg) were divided into 5 groups. The birds were fed diets supplemented with 0 mg/kg selenium (Se) (CON), 0.3 mg/kg Se from sodium selenite (SS), 0.3 mg/kg Se from Se-enriched yeast (SEY), 0.3 mg/kg Se for selenium-enriched Cardamine violifolia (SEC) or 0.3 mg/kg Se from Se-enriched Cardamine violifolia and 0.3 mg/kg Se from Se-enriched yeast (SEC + SEY) for 8 weeks. The eggs were collected on the 8th week and were analyzed for egg quality and oxidative stability of yolk during storage at 4 °C or 25 °C for 0, 2, 4, or 6 weeks. Dietary SEC and SEC + SEY supplementation increased the Haugh unit (HU) and albumen foam stability in eggs stored at 4 °C and 25 °C (p < 0.05). SS and SEC supplementation increased the yolk index in eggs stored at 25 °C (p < 0.05). SEC or SEC + SEY slowed down an increase in albumen pH and gel firmness in eggs stored at 4 °C and 25 °C (p < 0.05). Moreover, SEC or SEC + SEY alleviated the increase in malonaldehyde (MDA), and the decrease in total antioxidant capacity (T-AOC) level and total superoxide dismutase (T-SOD) activity in yolks stored at 4 °C and 25 °C (p < 0.05). These results indicate that SEC mitigated egg quality loss and improved the antioxidant capacity of yolks during storage. SEC supplementation would be advantageous to extend the shelf life of eggs.
摘要：
pH-responsive intelligent films for food freshness monitoring have attracted great attentions recently. In this study, several intelligent films based on chitosan (CS), polyvinyl alcohol (PVA), and grape skin anthocyanin (GSA) were prepared, and the effect of film-forming solution pH on the properties of intelligent films was investigated. The results of SEM, FTIR, XRD and TGA displayed that the hydrogen bond between CS and GSA was strong at strong acidic conditions (2.0-2.5), and it weakened at weak acidic conditions (3.0-4.5). Meanwhile, the hydrogen bond between PVA and GSA was negligible under strong acidic conditions, and it appeared under weak acidic conditions. Consequently, the films fabricated under weak acidic conditions displayed lower water solubility, lower water vapor permeability, and higher elongation at break. The tensile strength of films increased firstly and subsequently decreased with pH increasing, reaching a maximum value of 31.44MPa at pH3.5. Additionally, the films prepared at pH2.5 and 4.0 showed the best color responsiveness to ammonia and acetic acid, respectively. Overall, the intelligent films prepared under variant pH have the potential to realize the goal of monitoring the freshness of different types of food, thereby expanding the application subject of anthocyanins-based intelligent films.
通讯机构：
[Zhu, ZZ ; Li, SY ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Wuhan 430023, Peoples R China.
关键词：
gluten protein;grape skin anthocyanin extract;microstructure and physicochemical properties
摘要：
Intake of polyphenol-modified wheat products has the potential to reduce the incidence of chronic diseases. In order to determine the modification effect of polyphenols on wheat gluten protein, the effects of grape skin anthocyanin extract (GSAE, additional amounts of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%, respectively) on the microstructure and physicochemical properties of gluten protein were investigated. The introduction of GSAE improves the maintenance of the gluten network and increases viscoelasticity, as evidenced by rheological and creep recovery tests. The tensile properties of gluten protein were at their peak when the GSAE level was 0.3%. The addition of 0.5% GSAE may raise the denaturation temperature of gluten protein by 6.48 °C-9.02 °C at different heating temperatures, considerably improving its thermal stability. Furthermore, GSAE enhanced the intermolecular hydrogen bond of gluten protein and promoted the conversion of free sulfhydryl groups to disulfide bonds. Meanwhile, the GSAE treatment may also lead to protein aggregation, and the average pore size of gluten samples decreased significantly and the structure became denser, indicating that GSAE improved the stability of the gluten spatial network. The positive effects of GSAE on gluten protein properties suggest the potential of GSAE as a quality enhancer for wheat products.
摘要：
Fungal symbionts can improve plant tolerance to salt stress. However, the interaction of epiphytic Fusarium oxysporum and Fusarium fujikuroi with the tolerance of the invasive plant Ipomoea cairica against saline coastal habitats is largely unknown. This study aimed to investigate the interaction of the mixture of the two epiphytic fungi with salt tolerance of I. cairica. Surface-sterilized I. cairica cuttings inoculated (E+) and non-inoculated (E-) with the fungal mixture were cultivated with 2, 3, and 5 parts per thousand (PPT) of NaCl solutions to simulate mild, moderate, and severe salt stress, respectively. The hydroponic experiment showed that the growth inhibition and peroxidation damages of E+ and E- cuttings were aggravated with salinity. Noteworthily, E+ cuttings had higher peroxidase (POD) and catalase (CAT) activities, chlorophyll content, total biomass, aboveground biomass, total shoot length and secondary shoot number, but lower root-to-shoot ratio than E- cuttings under 2 and 3 PPT NaCl conditions. Moreover, E+ had higher superoxide dismutase (SOD) activity and proline content but lower belowground biomass and malondialdehyde (MDA) content than E- cuttings under 3 PPT NaCl condition. However, lower SOD, POD, and CAT activities, and chlorophyll content, but higher MDA content occurred in E+ cuttings than in E- cuttings under 5 PPT NaCl condition. These findings suggested that the mixture of the two epiphytic fungi increased salt tolerance of I. cairica mainly through increasing its antioxidation ability and chlorophyll stability under mildly and moderately saline conditions, but decreased salt tolerance of this plant in an opposite way under severely saline conditions.
期刊：
International Journal of Biological Macromolecules,2024年260(Pt 1):129329 ISSN：0141-8130
作者机构：
[Cai, Jie; Yang, Zhaoxing; Zhang, Die] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Cai, Jie] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: caijievip@whpu.edu.cn
关键词：
Food packaging;Starch;Tannic acid
摘要：
Developing efficient and biodegradable packaging films is of paramount significance owing to the scarcity of petroleum based resources. However, their applications in food packaging are limited due to their poor mechanical properties and inadequate biological activities. This study proposes a novel approach to develop the starch composite nanofibrous films (SNFs/TA/Fe(3+)) consisting of starch, tannic acid, and Fe(3+) using the temperature-assisted electrospinning method. The addition of TA resulted in a decrease in the rate of thermal degradation, indicating an improvement in the thermal stability of SNFs. However, the incorporation of TA or TA/Fe(3+) showed only a slight impact on the internal structure of SNFs. SNFs/TA/Fe(3+) loaded with 0.1wt% of Fe(3+) demonstrated a significantly higher tensile strength compared to SNFs and those loaded with TA alone. The presence of TA enhances the antioxidant activity of SNFs, and the robust SNFs/TA/Fe(3+) exhibited comparable antioxidant activity to SNFs/TA. However, the SNFs/TA/Fe(3+) showed a reduction in antibacterial activity, possibly due to the high valence state of the metal ions. Overall, these findings highlighted that a simple electrospinning method was used to produce SNFs/TA/Fe(3+) resulted in improved mechanical properties and antioxidant activity, offering a new strategy for the development of active food packaging using SNFs.
作者机构：
[Hu, Xiaosong; Li, Daotong; Ma, Chen] College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Beijing Key Laboratory for Food Non-Thermal Processing, China Agricultural University, Beijing 100083, China;National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;Author to whom correspondence should be addressed.;[Chen, Fang] College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Beijing Key Laboratory for Food Non-Thermal Processing, China Agricultural University, Beijing 100083, China<&wdkj&>Author to whom correspondence should be addressed.;[Wang, Xuehua] College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Beijing Key Laboratory for Food Non-Thermal Processing, China Agricultural University, Beijing 100083, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Fang Chen] C;College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Beijing Key Laboratory for Food Non-Thermal Processing, China Agricultural University, Beijing 100083, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
SCFAs;gut microbiota;high hydrostatic pressure;metabolic profiles;tomato juice
摘要：
High hydrostatic pressure (HHP) is a non-thermal pasteurization technology for the enhancement of food products' safety and quality. The components of tomato juice can be affected by HHP processing. Little is known about the effects of HHP-processed tomato juice on the gut microbiome and metabolism. Here, we performed high-throughput sequencing and metabolomics profiling to determine the critical differences in gut microbiota structure and metabolic profiles in mice administered with HHP-processed tomato juice. Tomato juice administration significantly increased the gut bacterial alpha diversity and the relative abundance of Bacteroides. The mice administered with HHP-processed tomato juice were characterized by the enrichment of Bacteroidetes, Alistieps, and Faecalibaculum compared with those administered with HTST-processed tomato juice. Moreover, HHP-processed tomato juice promoted SCFA levels, which were positively correlated with the enriched Alistieps. Our results show that HHP-processed tomato juice may drive healthy gut microbes and metabolites.
通讯机构：
[Wu, Q; Wang, C ] H;Hubei Univ Technol, Coll Bioengn & Food, Wuhan 430068, Peoples R China.
关键词：
Feruloylated arabinoxylan;Pea starch;Processing and digestibility properties
摘要：
In this study, arabinoxylans (AX) with various molecular weights (Mw) and bound ferulic acid (FA) contents were prepared to compare their effects on the gelatinization, short-term retrogradation and digestive properties of pea starch (PeS). The results indicated that all AX samples could obviously impede the pasting process of PeS and inhibit the short-term retrogradation of PeS-based gels during refrigeration by hindering the rearrangement and double helical associations of amylose. More precisely, AXs with low Mw and the highest FA content (H-FAX) exhibited the strongest intervention ability on PeS compared with the other samples. According to the Fourier transform infrared spectroscopy and X-ray diffraction results, it might be due to the unique role of bound FA as a noncovalent cross-linking agent, which enhanced the association between AX and starch molecules through extra hydrogen bonding interactions and entanglement behaviour. On these bases, H-FAX clearly improved the hardness, chewiness, moisture content, and sensory acceptance of PeS-base gels (pea jelly), and could also regulate its starch composition during short-term refrigeration to delay starch digestion. Overall, AXs with appropriate structural features might obviously improve the quality and storage stability of PeS-based foods.
摘要：
Cardamine violifolia is a unique selenium hyperaccumulating vegetable in China, but its flowers are commonly wasted in large-scale cultivation. To better utilize this resource, this study explored the selenium content, chemical composition, and volatile organic compounds (VOCs) of hydro-distilling essential oil (EO) and hydrosol from C. violifolia flowers. ICP-MS results indicated that the EO and hydrosol contained selenium reaching 13.66±2.82 mg/kg and 0.0084±0.0013 mg/kg, respectively. GC-MS analysis revealed that organic acids, hydrocarbons, and amines were the main components of EO. Additionally, benzyl nitrile, benzaldehyde, benzyl isothiocyanate, benzyl alcohol, megastigmatrienone, and 2-methoxy-4-vinylphenol also existed in considerable amounts. The hydrosol extract had fewer components, mainly amines. HS-SPME-GC-MS corresponded to the composition analysis and aromatic compounds were the prevalent VOCs, while HS-GC-IMS primarily identified C2-C10 molecular alcohols, aldehydes, ethers, and sulfur-containing compounds. This study first described the chemical composition and VOC profiles of EO and hydrosol from selenium hyperaccumulating plant.
作者机构：
[Cao, Jie; Xu, Feng; Wang, Li-Na; Zhou, Xian; Zhang, Wei-wei; Ye, Jia-Bao] Yangtze Univ, Coll Hort & Gardening, Jingzhou, Peoples R China.;[Cheng, Shuiyuan; Liu, Xiao-Meng] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;[Xu, Feng; Xu, F] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.
通讯机构：
[Xu, F ] Y;Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.
摘要：
4-Coumarate-CoA ligase (4CL) gene plays vital roles in plant growth and development, especially the regulation of lignin metabolism and flavonoid synthesis. To investigate the potential function of 4CL in the lignin biosynthesis of Ginkgo biloba, this study identified two 4CL genes, Gb4CL1 and Gb4CL2, from G. biloba genome. Based on the phylogenetic tree analysis, Gb4CL1 and Gb4CL2 protein were classified into Class I, which has been confirmed to be involved in lignin biosynthesis. Therefore, it can be inferred that these two genes may also participate in lignin metabolism. The tissue-specific expression patterns of these two genes revealed that Gb4CL1 was highly expressed in microstrobilus, whereas Gb4CL2 was abundant in immature leaves. The onion transient expression assay indicated that Gb4CL1 was predominantly localized in the nucleus, indicating its potential involvement in nuclear functions, while Gb4CL2 was observed in the cell wall, suggesting its role in cell wall-related processes. Phytohormone response analysis revealed that the expression of both genes was upregulated in response to indole acetic acid, while methyl jasmonate suppressed it, gibberellin exhibited opposite effects on these genes. Furthermore, Gb4CL1 and Gb4CL2 expressed in all tissues containing lignin that showed a positive correlation with lignin content. Thus, these findings suggest that Gb4CL1 and Gb4CL2 are likely involved in lignin biosynthesis. Gb4CL1 and Gb4CL2 target proteins were successfully induced in Escherichia coli BL21 with molecular weights of 85.5 and 89.2kDa, proving the integrity of target proteins. Our findings provided a basis for revealing that Gb4CL participated in lignin synthesis in G. biloba.
摘要：
The use of microalgae-bacteria consortia (MBC) for wastewater treatment has garnered attention as their interactions impart greater environmental adaptability and stability compared with that obtained by only microalgae or bacteria use, thereby improving the efficiency of pollutant removal and bio-product productivity. Additionally, the value-added bio-products produced via biorefineries can improve economic competitiveness and environmental sustainability. Therefore, this review focuses on the interaction between microalgae and bacteria that leads to nutrient exchange, gene transfer and signal transduction to comprehensively understand the interaction mechanisms underlying their strong adaptability. In addition, it includes recent research in which MBC has been efficiently used to treat various wastewater. Moreover, the review summarizes the use of MBC-produced biomass in a biorefining context to produce biofuel, biomaterial, high-value bio-products and bio-fertilizer. Overall, more effort is needed to identify the symbiotic mechanism in MBC to provide a foundation for circular bio-economy and environmentally friendly development programmes.
作者机构：
National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China;School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China;Authors to whom correspondence should be addressed.;[Wenxin Guo; Shuiyuan Cheng; Xinyu Shi; Xin Cong; Lu Wang] National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China;[Hua Cheng; Linling Li] Authors to whom correspondence should be addressed.<&wdkj&>National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China
通讯机构：
[Hua Cheng; Linling Li] A;Authors to whom correspondence should be addressed.<&wdkj&>National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China
摘要：
Selenium nanoparticles (SeNPs) have greater bioavailability and safety than inorganic selenium, and was widely used in medical, agricultural, nutritional supplements, and antibacterial fields. The present study screened a strain L11 producing SeNPs from a selenium rich dairy cow breeding base in Hubei Province, China. The strain was identified as Bacillus subtilis through physiological, biochemical, and molecular biology analysis. By adjusting the cultivation conditions, the experiment determined the ideal parameters for L11 to efficiently produce SeNPs. These parameters include a pH value of 6, a cultivation temperature of 37 °C, a concentration of 4 mmol/L Na2SeO3, and a cultivation of 48 h. X-ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDS), and Transmission Electron Microscopy (TEM) were used to verify that the Se particles produced by L11 are SeNPs with diameters ranging from 50 to 200 nm. The combination of the protein analysis of different cell components and TEM analysis showed that L11 mainly produces SeNPs through the transformation of the cell’s periplasmic space, cell membrane, and cell wall. Adding the L11 SeNPs complex to sheep feed can significantly enhance the antioxidant activity and immunity of sheep, and increase the Se content in the neck muscles, liver, and spleen tissues.
期刊：
Journal of Food Protection,2024年87(4):100244 ISSN：0362-028X
通讯作者：
Chen, Xuehong
作者机构：
[Li, Shuyao; Zhang, Yu; Li, Ru; Feng, Xingxing; Yuan, Heng; Sun, Zifan; Yu, Nannan] School of Food and Bio-engineering, Xuzhou University of Technology, 221018 Xuzhou, China;[Li, Shuyao] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, 430023 Wuhan, China;[Chen, Xuehong] School of Food and Bio-engineering, Xuzhou University of Technology, 221018 Xuzhou, China. Electronic address: xzcxhong@163.com
通讯机构：
[Chen, Xuehong] S;School of Food and Bio-engineering, Xuzhou University of Technology, 221018 Xuzhou, China. Electronic address:
摘要：
Strawberries rapidly deteriorate postharvest, necessitating effective measures to extend their shelf life. This study focused on developing an eco-friendly chitosan-based protective film for strawberry preservation. Strawberries were treated with a coating solution containing varying concentrations of hawthorn leaf extract (HLE) (0.4%, 0.7%, and 1.0%), 1.5% chitosan (CH), and 1% acetic acid. The results demonstrated that coating strawberry fruit with 1% CH-HLE notably delayed fruit spoilage. In-depth analysis revealed that, compared with the uncoated strawberry fruits, the 1% CH-HLE coating effectively reduced weight loss, the respiration intensity, malondialdehyde (MDA) levels, and superoxide anion (O(2)(·-)) production. Additionally, the coated strawberries exhibited improved firmness, total soluble solids (TSS), vitamin C (Vc) content, titratable acidity (TA), and total phenolic compound (TPC) content. The enzyme activities of superoxide dismutase (SOD) and catalase (CAT) in the CH-HLE-coated strawberries were greater than those in their uncoated counterparts. The application of a 1% CH-HLE coating successfully delayed spoilage and extend the shelf life of the strawberries by approximately 4-5days. These findings suggest that CH-HLE has significant potential as a resource for protecting fruits and vegetables, offering an environmentally sustainable solution for postharvest preservation.
期刊：
European Journal of Pharmacology,2024年964:176226 ISSN：0014-2999
通讯作者：
Cheng, SY;Liu, Q
作者机构：
[Li, Xiaoqian; Zhang, Huajie; He, Zhijun; Li, Nan; Liu, Qiong; Shen, Liming] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Biotechnol & Ecol, Shenzhen 518055, Guangdong, Peoples R China.;[Cheng, Shuiyuan; Cheng, SY; He, Zhijun] Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Wuhan 430023, Peoples R China.;[Li, Nan; Liu, Qiong] Shenzhen Hong Kong Inst Brain Sci Shenzhen Fundame, Shenzhen 518055, Peoples R China.;[Liu, Qiong] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen 518055, Peoples R China.
通讯机构：
[Liu, Q ] S;[Cheng, SY ] W;Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Wuhan 430023, Peoples R China.;Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen 518055, Peoples R China.
关键词：
Alzheimer's disease (AD);Cerebral cortex;Esculentoside A (EsA);Proteomics
摘要：
Esculentoside A (EsA), isolated from phytolacca esculenta, is a saponin showing neuroprotective effect in the mouse models of Alzheimer's disease (AD). To investigate its action target and underlying mechanism, this study used the proteomics technique of isobaric tags for relative and absolute quantification (iTRAQ) to analyze the differentially expressed proteins (DEPs) in the cerebral cortex of EsA-treated and untreated triple-transgenic 3×Tg-AD model mice. Proteomic comparison revealed 250, 436, and 903 DEPs in three group pairs, i.e. AD/Wild-type (WT), AD+5mg/kg EsA/AD, AD+10mg/kg EsA/AD, respectively. Among them 28 DEPs were commonly shared by three group pairs, and 25 of them showed reversed expression levels in the diseased group under the treatment of both doses of EsA. Bioinformatics analysis revealed that these DEPs were mainly linked to metabolism, synapses, apoptosis, learning and memory. EsA treatment restored the expression of these proteins, including amyloid precursor protein (APP), cathepsin B (Cstb), 4-aminobutyrate aminotransferase (Abat), 3-phosphoinositide-dependent protein kinase-1 (PDK1), carnitine palmitoyltransferase1 (Cpt1) and synaptotagmin 17 (Syt17), thereby ameliorated the spatial learning and memory of AD mice. Collectively, this study reveals for the first time the profound effect of EsA on the cerebral cortex of AD mice, which might be a potential therapeutic agent for the treatment of AD.
作者机构：
Authors to whom correspondence should be addressed.;[Wangting Zhou; Jia Li; Meng Xu; Muci Wu; Jinjing Yin] National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China;[Xinsun Yang] Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China;[Rui Zhang] Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Rui Zhang] A;[Jingren He] K;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Purple sweet potato (PSP) has abundant nutritional compounds, which are valuable constituents of the human diet, but its development and utilization are still in the primary processing phase. This study examined the differences in nutritional characteristics of 10 PSP varieties. A variety of nutritional components were evaluated and comprehensively compared using principal component analysis (PCA) and cluster analysis (CA). The PSP had 60.9–70.1% moisture. The dried PSP had abundant starch (43.9~67.2%) and dietary fiber (9.40~16.5%), moderate levels of protein (3.19~8.75%) and reducing sugar (1.44~4.01%), and low amounts of crude fat (0.51~1.01%). The anthocyanin profile varied significantly between the different varieties. A correlation analysis showed that a higher content of anthocyanins resulted in a darker color. The PCA and CA suggested that varieties XS, ZL, and JS18 are desirable for developing the diabetic patient’s diet. JS1 had the highest anthocyanin, protein, and dietary fiber contents and the lowest starch, implying that it could be used as a source of natural colorants or functional foods. Varieties FX, GS, ES13, and EN are suitable for producing various starch-based food products, such as noodles, cookies, and pastries. This study provides a reference for the practical use and rational processing of PSP resources.
关键词：
Chinese quince;Growth stages;Proanthocyanidins;Chemical composition;Structure characterization;Antioxidant capacity
摘要：
This study analyzed the chemical composition and antioxidant properties of proanthocyanidins (PAs) in Chinese quince fruits at six growth stages. UPLC-QTOF-ESI-MS/MS and MALDI-TOF-MS identified and quantified the chemical composition, while radical scavenging ability assessed antioxidant capacities. PA concentration peaked during mid-growth and declined as fruits ripened. The highest total proanthocyanidin content occurred in August (1776.69 +/- 56.97 mg PB2/100 g FW). Catechin and epicatechin were the primary PA subunits, forming trimers to tetradecamers and higher degrees of PAs. The PA extracts were composed predominantly of highly polymerized B-type procyanidins with degrees of polymerization (DP) greater than 4, which accounted for over 93% of total PA content, along with minor amounts of dimeric and trimeric prodelphinidins. Molecular weight of PA samples decreased as fruits ripened. The highest antioxidant activities against DPPH+center dot and ABTS(+)center dot were observed in July (IC50 = 7.17 +/- 0.073 mu g/mL) and August (IC50 = 67.47 +/- 2.29 mg/mL), respectively, with the highest FRAP activity in July. Immature fruits with elevated PA content and antioxidant capacity show promising potential for functional food applications.
摘要：
Selenium-enriched Cardamine violifolia (SEC), a cruciferous plant, exerts excellent antioxidant and anti-inflammatory capacity, but its effect on hepatic function is unclear. This study investigated the effect and potential mechanism of SEC on hepatic injury induced by lipopolysaccharide (LPS). Twenty-four weaned piglets were randomly allotted to treatment with SEC (0.3 mg/kgSe) and/or LPS (100 μg/kg). After 28 days of the trial, pigs were injected with LPS to induce hepatic injury. These results indicated that SEC supplementation attenuated LPS-induced hepatic morphological injury and reduced aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities in plasma. SEC also inhibited the expression of pro-inflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) after the LPS challenge. In addition, SEC improved hepatic antioxidant capacity via enhancing glutathione peroxidase (GSH-Px) activity and decreasing malondialdehyde (MDA) concentration. Moreover, SEC downregulated the mRNA expression of hepatic myeloid differentiation factor 88 (MyD88) and nucleotide-binding oligomerization domain proteins 1 (NOD1) and its adaptor molecule receptor interacting protein kinase 2 (RIPK2). SEC also alleviated LPS-induced hepatic necroptosis by inhibiting RIPK1, RIPK3, and mixed-lineage kinase domain-like (MLKL) expression. These data suggest that SEC potentially mitigates LPS-induced hepatic injury via inhibiting Toll-like receptor 4 (TLR4)/NOD2 and necroptosis signaling pathways in weaned piglets.
作者机构：
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan, 430068, China;School of Life and Health Sciences, Hubei University of Technology, Wuhan, 430068, China;[Muci Wu] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[Jingyi Wang; Shumin He; Sihong Tao; Shuzhen Ma; Yufan Luo; Mengzhou Zhou] Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan, 430068, China<&wdkj&>School of Life and Health Sciences, Hubei University of Technology, Wuhan, 430068, China
通讯机构：
[Mengzhou Zhou] C;Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan, 430068, China<&wdkj&>School of Life and Health Sciences, Hubei University of Technology, Wuhan, 430068, China
摘要：
This study explores the effect of ultrasound-assisted cellulase (UE) processing on the structure, functional performance, and prebiotic properties of guava insoluble dietary fiber (RIDF). As the ultrasound exposure time increased (0–40 min), the particle size (from 126.3 μm to 38.7 μm) and crystallinity (from 30.72% to 14.42%) of RIDF significantly decreased, while its soluble carbohydrate content (from 1.40% to 6.17%) and water solubility (from 8.6% to 16.4%) notably increased. These crucial changes suggested the degradation and disorganization of RIDF. Meanwhile, UE-RIDF (modified RIDF) demonstrated a honeycomb network microstructure with a larger surface area, indicating distinct structural and morphological properties compared to RIDF. Consequently, UE-RIDF showed stronger retention/adsorption capacity and improved in vitro prebiotic activity. Interestingly, UE treatment increased RIDF fermentability, leading to additional shifts in its modulation of human fecal microbiota, including a higher abundance of Proteobacteria and Phascolarctobacterium. Furthermore, compared to pure cow/goat yogurt, yogurt fermented with UE-RIDF displayed a lower pH, better stability, and improved texture and rheological properties during a 21-day refrigeration period. These findings highlight the potential application of RIDF as a novel prebiotic active ingredient in fermented foods for the first time, underscoring the critical role of UE treatment in enhancing its performance.
