作者机构：
School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, PR China;National R&D Center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan, 430023, PR China;[Chen, Xu; Xiang, Hongxia; Liang, Yong; He, Jiangling; Chen, Rongyu; Zhu, Zhenzhou; Li, Shuyi; Chen, Xiang; Cheng, Shuiyuan] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, PR China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan, 430023, PR China
通讯机构：
[Xu Chen] S;School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, PR China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan, 430023, PR China
摘要：
Highly stable and multifunctional intelligent films were fabricated using a combination of grape skin anthocyanin, polyvinyl alcohol, chitosan and selenopeptide, and the influence of selenopeptide concentration on films' properties and their effectiveness in strawberry preservation and freshness monitoring was investigated. The results showed that the incorporated selenopeptide could interact with polyvinyl alcohol and grape skin anthocyanin via hydrogen bonding, improving the hydrophobic, UV-blocking, and mechanical characteristics of the films. In particular, the introduction of 0.4% selenopeptide into the film notably boosted its tensile strength from 11.91 MPa to 26.91 MPa. More importantly, the films incorporated with selenopeptide showed decent antioxidant and antibacterial properties, along with high storage stability and thermal stability. Adding 0.4% selenopeptide to the film can increase the shelf life of strawberries at 25 °C by 3 days, and a distinct color change showing fair good linear relationship with the freshness indexes of the strawberry (hardness and weight loss rate) was observed. Overall, anthocyanin-infused films combined with selenopeptide have demonstrated promising results in preserving food and monitoring freshness, opening up new opportunities for preparing stable, smart and active films.
摘要：
<jats:p>The chemical composition discrepancies of five sweet potato leaves (SPLs) and their phenolic profile variations during in vitro digestion were investigated. The results indicated that Ecaishu No. 10 (EC10) provided better retention capacity for phenolic compounds after drying. Furthermore, polyphenols were progressively released from the matrix as the digestion process proceeded. The highest bioaccessibility of polyphenols was found in EC10 intestinal chyme at 48.47%. For its phenolic profile, 3-, 4-, and 5-monosubstituted caffeoyl quinic acids were 9.75%, 57.39%, and 79.37%, respectively, while 3,4-, 3,5-, and 4,5-disubstituted caffeoyl quinic acids were 6.55, 0.27 and 13.18%, respectively. In contrast, the 3,4-, 3,5-, 4,5-disubstituted caffeoylquinic acid in the intestinal fluid after dialysis bag treatment was 62.12%, 79.12%, and 62.98%, respectively, which resulted in relatively enhanced bioactivities (DPPH, 10.51 μmol Trolox/g; FRAP, 8.89 μmol Trolox/g; ORAC, 7.32 μmol Trolox/g; IC50 for α-amylase, 19.36 mg/g; IC50 for α-glucosidase, 25.21 mg/g). In summary, desirable phenolic acid release characteristics and bioactivity of EC10 were observed in this study, indicating that it has potential as a functional food ingredient, which is conducive to the exploitation of the sweet potato processing industry from a long-term perspective.</jats:p>
作者机构：
[Cai, Jie; Xie, F; Xie, Fang; Zhang, Die] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Zhang, Die] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J; Xie, F ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
摘要：
Cardamine violifolia is a significant Brassicaceae plant known for its high selenium (Se) accumulation capacity, serving as an essential source of Se for both humans and animals. WRKY transcription factors play crucial roles in plant responses to various biotic and abiotic stresses, including cadmium stress, iron deficiency, and Se tolerance. However, the molecular mechanism of CvWRKY in Se accumulation is not completely clear. In this study, 120 WRKYs with conserved domains were identified from C. violifolia and classified into three groups based on phylogenetic relationships, with Group II further subdivided into five subgroups. Gene structure analysis revealed WRKY variations and mutations within the CvWRKYs. Segmental duplication events were identified as the primary driving force behind the expansion of the CvWRKY family, with numerous stress-responsive cis-acting elements found in the promoters of CvWRKYs. Transcriptome analysis of plants treated with exogenous Se and determination of Se levels revealed a strong positive correlation between the expression levels of CvWRKY034 and the Se content. Moreover, CvWRKY021 and CvWRKY099 exhibited high homology with AtWRKY47, a gene involved in regulating Se accumulation in Arabidopsis thaliana. The WRKY domains of CvWRKY021 and AtWRKY47 were highly conserved, and transcriptome data analysis revealed that CvWRKY021 responded to Na2SeO4 induction, showing a positive correlation with the concentration of Na2SeO4 treatment. Under the induction of Na2SeO3, CvWRKY021 and CvWRKY034 were significantly upregulated in the roots but downregulated in the shoots, and the Se content in the roots increased significantly and was mainly concentrated in the roots. CvWRKY021 and CvWRKY034 may be involved in the accumulation of Se in roots. The results of this study elucidate the evolution of CvWRKYs in the C. violifolia genome and provide valuable resources for further understanding the functional characteristics of WRKYs related to Se hyperaccumulation in C. violifolia.
摘要：
Selenium (Se)-rich Cyclocarya paliurus is popular for its bioactive components, and exogenous Se fortification is the most effective means of enrichment. However, the effects of exogenous Se fortification on the nutritional quality of C. paliurus are not well known. To investigate the nutrient contents and antioxidant properties of C. paliurus following Se treatment, we used a foliar spray to apply Se in two forms-chemical nano-Se (Che-SeNPs) and sodium selenite (Na(2)SeO(3)). Sampling began 10 days after spraying and was conducted every 5 days until day 30. The Se, secondary metabolite, malondialdehyde contents, antioxidant enzyme activity, Se speciation, and Se-metabolism-related gene expression patterns were analyzed in the collected samples. Exogenous Se enhancement effectively increased the Se content of leaves, reaching a maximum on days 10 and 15 of sampling, while the contents of flavonoids, triterpenes, and polyphenols increased significantly during the same period. In addition, the application of Se significantly enhanced total antioxidant activity, especially the activity of the antioxidant enzyme peroxidase. Furthermore, a positive correlation between the alleviation of lipid peroxidation and Se content was observed, while methylselenocysteine formation was an effective means of alleviating Se stress. Finally, Na(2)SeO(3) exhibited better absorption and conversion efficiency than Che-SeNPs in C. paliurus.
作者机构：
[Cheng, Shuiyuan; He, Yi; Zhu, Lisha; Dong, Xingxing; Long, Pengcheng; He, Y; Hu, Man] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[He, Yi; Zhu, Lisha] Wuhan Polytech Univ, Sch Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod,Minist E, Wuhan 430023, Peoples R China.;[Wang, Liling] Tarim Univ, Coll Food Sci & Engn, Alar 843300, Peoples R China.;[Shao, Yanchun] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.
通讯机构：
[He, Y ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
摘要：
Monascus species are functional fermentation fungi with great potential for selenium (Se) supplementation. This study investigated the effects of Se bio-fortification on the growth, morphology, and biosynthesis of Monascus ruber M7. The results demonstrated a significant increase in the yield of orange and red Monascus pigments (MPs) in red yeast rice (RYR) by 38.52% and 36.57%, respectively, under 20μg/mL of selenite pressure. Meanwhile, the production of citrinin (CIT), a mycotoxin, decreased from 244.47μg/g to 175.01μg/g. Transcriptome analysis revealed significant upregulation of twelve genes involved in MPs biosynthesis, specifically MpigE, MpigF, and MpigN, and downregulation of four genes (mrr3, mrr4, mrr7, and mrr8) associated with CIT biosynthesis. Additionally, three genes encoding cysteine synthase cysK (Log(2)FC=1.6), methionine synthase metH (Log(2)FC=2.2), and methionyl-tRNA synthetase metG (Log(2)FC=1.8) in selenocompound metabolism showed significantly upregulated. These findings provide insights into Se biotransformation and metabolism in filamentous fungi.
摘要：
Cassava serves as a primary staple food for over one billion people worldwide. The quality of cassava flour is markedly affected by the oxidation and deterioration of lipids during storage. Despite its significance, the lipid composition of cassava flour and its alterations throughout storage periods have not been extensively studied. This study offers a comprehensive lipidomic analysis of cassava flour over storage periods using liquid chromatography–mass spectrometry/mass spectrometry (LC-MS/MS). The results showed that 545 lipids from five classes and 27 subclasses were identified in cassava flour, including key substances such as free fatty acids (36 species), diglycerides (DGs) (31 species), and triglycerides (TGs) (259 species). Using Metware Cloud for statistical analysis, significant variations were observed in 50 lipid species over long-term storage, reflecting changes in lipid profiles due to storage. These lipids correlate with seven metabolic pathways, among which glycerolipid metabolism is the most affected. The metabolites associated with these pathways can differentiate cassava flour based on the length of storage. This study provides a theoretical basis and storage technology parameters for lipid changes during cassava flour storage.
摘要：
Acting as a growth regulator, Indole-3-acetic acid (IAA) is an important phytohormone that can be produced by several Bacillus species. However, few studies have been published on the comprehensive evaluation of the strains for practical applications and the effects of selenium species on their IAA-producing ability. The present study showed the selenite reduction strain Bacillus altitudinis LH18, which is capable of producing selenium nanoparticles (SeNPs) at a high yield in a cost-effective manner. Bio-SeNPs were systematically characterized by using DLS, zeta potential, SEM, and FTIR. The results showed that these bio-SeNPs were small in particle size, homogeneously dispersed, and highly stable. Significantly, the IAA-producing ability of strain was differently affected under different selenium species. The addition of SeNPs and sodium selenite resulted in IAA contents of 221.7 mu g/mL and 91.01 mu g/mL, respectively, which were 3.23 and 1.33 times higher than that of the control. This study is the first to examine the influence of various selenium species on the IAA-producing capacity of Bacillus spp., providing a theoretical foundation for the enhancement of the IAA-production potential of microorganisms.
摘要：
Oxidative stress occurs in the process of egg storage. Antioxidants as feed additives can enhance egg quality and extend the shelf life of eggs. Selenium-enriched Cardamine violifolia (SEC) has strongly antioxidant properties. The objective of this study was to assess the effects of dietary supplementation with SEC on egg quality and the yolk antioxidant capacity of eggs stored at 4 degrees C and 25 degrees C. Four hundred fifty 65-week-old, Roman hens that were similar in laying rate (90.79 +/- 1.69%) and body weight (2.19 +/- 0.23 kg) were divided into 5 groups. The birds were fed diets supplemented with 0 mg/kg selenium (Se) (CON), 0.3 mg/kg Se from sodium selenite (SS), 0.3 mg/kg Se from Se-enriched yeast (SEY), 0.3 mg/kg Se for selenium-enriched Cardamine violifolia (SEC) or 0.3 mg/kg Se from Se-enriched Cardamine violifolia and 0.3 mg/kg Se from Se-enriched yeast (SEC + SEY) for 8 weeks. The eggs were collected on the 8th week and were analyzed for egg quality and oxidative stability of yolk during storage at 4 degrees C or 25 degrees C for 0, 2, 4, or 6 weeks. Dietary SEC and SEC + SEY supplementation increased the Haugh unit (HU) and albumen foam stability in eggs stored at 4 degrees C and 25 degrees C (p < 0.05). SS and SEC supplementation increased the yolk index in eggs stored at 25 degrees C (p < 0.05). SEC or SEC + SEY slowed down an increase in albumen pH and gel firmness in eggs stored at 4 degrees C and 25 degrees C (p < 0.05). Moreover, SEC or SEC + SEY alleviated the increase in malonaldehyde (MDA), and the decrease in total antioxidant capacity (T-AOC) level and total superoxide dismutase (T-SOD) activity in yolks stored at 4 degrees C and 25 degrees C (p < 0.05). These results indicate that SEC mitigated egg quality loss and improved the antioxidant capacity of yolks during storage. SEC supplementation would be advantageous to extend the shelf life of eggs.
摘要：
pH-responsive intelligent films for food freshness monitoring have attracted great attentions recently. In this study, several intelligent films based on chitosan (CS), polyvinyl alcohol (PVA), and grape skin anthocyanin (GSA) were prepared, and the effect of film-forming solution pH on the properties of intelligent films was investigated. The results of SEM, FTIR, XRD and TGA displayed that the hydrogen bond between CS and GSA was strong at strong acidic conditions (2.0-2.5), and it weakened at weak acidic conditions (3.0-4.5). Meanwhile, the hydrogen bond between PVA and GSA was negligible under strong acidic conditions, and it appeared under weak acidic conditions. Consequently, the films fabricated under weak acidic conditions displayed lower water solubility, lower water vapor permeability, and higher elongation at break. The tensile strength of films increased firstly and subsequently decreased with pH increasing, reaching a maximum value of 31.44MPa at pH3.5. Additionally, the films prepared at pH2.5 and 4.0 showed the best color responsiveness to ammonia and acetic acid, respectively. Overall, the intelligent films prepared under variant pH have the potential to realize the goal of monitoring the freshness of different types of food, thereby expanding the application subject of anthocyanins-based intelligent films.
期刊：
Food and Bioprocess Technology,2024年:1-13 ISSN：1935-5130
通讯作者：
Li, SY;Cong, X
作者机构：
[Li, Shuyi; Chen, Rongyu; Zhu, Zhenzhou; Wang, Xuehua; Gao, Weilan; Zhang, Na; Li, SY; Zheng, Zhan] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Peoples R China.;[Gao, Weilan] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.
通讯机构：
[Cong, X ] E;[Li, SY ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Peoples R China.;Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.
关键词：
Selenylation;Tea polysaccharides;Pulsed electric field;Ultrasound;Antioxidant capacities;Hypoglycemic activity
摘要：
Selenylation modification is an effective way to improve the nutritional and health effects of tea polysaccharide, but the effects of different selenylation methods on the structure and efficacy of polysaccharides remain unclear. In this study, a series of chemically selenized tea polysaccharides (CSe-TPS, selenium content of 129.98 similar to 1454.99 mu g/g) were prepared by Na2SeO3-HNO3 method, which were promoted by heating (HCSe-TPS), ultrasound (UCSe-TPS), and pulse electric field (PCSe-TPS) treatments, respectively. These different CSe-TPSs were systematically compared with ordinary tea polysaccharides (Ord-TPS) and natural selenium-enriched tea polysaccharides (NSe-TPS) in aspect of molecular structure and biological functions. The results of molecular structure and apparent morphology showed that the particle size (p < 0.05) and the thermal stability of Ord-TPS were reduced after selenylation modification, while the absolute potential of polysaccharide was increased (p < 0.05), coupled with altered monosaccharide composition and changed apparent morphology. However, the main functional groups and the secondary structure of Ord-TPS did not change. The antioxidant activities of tea polysaccharides after selenylation were improved as well as their inhibitory effects on carbohydrate digestive enzymes (p < 0.05). The total antioxidant capacity of PCSe-TPS (selenium content of 240.66 +/- 1.43 mu mol/g) was enhanced by 85.41% compared with Ord-TPS (selenium content of 129.80 +/- 3.42 mu mol/g), while its IC50 values on inhibition of alpha-amylase (2.91 +/- 0.18 mg/mL) and alpha-glucosidase (0.18 +/- 0.01 mg/mL) were significantly lower than other tea polysaccharides (p < 0.05), which suggested best antioxidant and hypoglycemic activity. Moreover, tea polysaccharides with higher selenium content and smaller particle size showed better antioxidant and hypoglycemic activities. This research will provide new strategies for the application of tea polysaccharides.
摘要：
Lettuce (Lactuca sativa) is rich in vitamins, minerals, and bioactive components, serving as an important source of selenium (Se) intake for humans. This study investigated the effects of Se treatment on lettuce using different concentrations of sodium selenite (Na2SeO3), focusing on biomass, physiological indicators, nutritional composition, and physiological changes during storage. Through correlation analysis of the transcriptome and Se species, the absorption and conversion mechanisms of Se in lettuce were revealed. The results showed that Se treatment initially increased the chlorophyll content in lettuce, followed by a decrease. Soluble sugar, soluble protein, total phenols, and anthocyanins increased at low Se concentrations but decreased at high concentrations. Flavonoid content decreased only at 1 mg/L Se, while other treatments were higher than the control group. GSH content and superoxide dismutase, catalase, and peroxidase activities initially increased and then decreased, while malondialdehyde (MDA) content first decreased and then increased. Five Se species, including Se (IV), Se (VI), selenocysteine (SeCys(2)), selenomethionine (SeMet), and methylselenocysteine (MeSeCys), were detected in lettuce leaves after Se treatment, with SeMet being the most abundant. During storage, Se-treated lettuce exhibited lower weight loss, a*, b*, browning index, and color difference (Delta E) values compared to the control group. CAT and POD activities and GSH content also followed a trend of initial increase followed by a decrease. Transcriptome data analysis revealed that genes such as MYB1, RPK1, PTR44, NTRC, WRKY7, and CSLD3 were associated with the stress response of Se-treated lettuce.
作者机构：
[Xie, Min; Lai, Yan; Fei, Dan; Zhou, Yaomin; Zhou, YM; Guang, Yelan] Jiangxi Acad Agr Sci, Inst Qual Safety & Stand Agr Prod, Key Lab Agroprod Qual & Safety Jiangxi Prov, Nanchang 330200, Jiangxi, Peoples R China.;[Xu, Jun] Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Med, Nanchang 330200, Jiangxi, Peoples R China.;[Wu, Na] Jiangxi Agr Univ, Coll Food Sci & Technol, Nanchang 330045, Jiangxi, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhou, YM ] J;Jiangxi Acad Agr Sci, Inst Qual Safety & Stand Agr Prod, Key Lab Agroprod Qual & Safety Jiangxi Prov, Nanchang 330200, Jiangxi, Peoples R China.
关键词：
Egg quality;Volatile organic compounds;Chemometric analysis;Odor activity value
摘要：
This study analyzed the egg quality of two commercial layers and two local breeds and detected volatile organic compounds (VOCs) using headspace solid-phase microextraction (HS-SPME-GC-MS) coupled with principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA). A total of 70 VOCs were identified in four types of eggs, mainly including aldehydes, alcohols, ketones, and esters. Chemometric and odor active value (OAV) analysis indicated that cis-3-hexen-1-ol, nonanal, n-decanal, benzaldehyde, and geranial were the main contributing components of flavor in eggs and can also serve as potential marker to distinguish different types of eggs. Both native eggs and commercial eggs have plentiful VOCs. This study contributed to a better understanding of the differences between commercial eggs and native eggs, laying a foundation for the development and utilization of native eggs.
摘要：
The study on the GmDWF1-deficient mutant dwf1 showed that GmDWF1 plays a crucial role in determining soybean plant height and yield by influencing the biosynthesis of brassinosteroids. Soybean has not adopted the Green Revolution, such as reduced height for increased planting density, which have proven beneficial for cereal crops. Our research identified the soybean genes GmDWF1a and GmDWF1b, homologous to Arabidopsis AtDWF1, and found that they are widely expressed, especially in leaves, and linked to the cellular transport system, predominantly within the endoplasmic reticulum and intracellular vesicles. These genes are essential for the synthesis of brassinosteroids (BR). Single mutants of GmDWF1a and GmDWF1b, as well as double mutants of both genes generated through CRISPR/Cas9 genome editing, exhibit a dwarf phenotype. The single-gene mutant exhibits moderate dwarfism, while the double mutant shows more pronounced dwarfism. Despite the reduced stature, all types of mutants preserve their node count. Notably, field tests have shown that the single GmDWF1a mutant produced significantly more pods than wild-type plants. Spraying exogenous brassinolide (BL) can compensate for the loss in plant height induced by the decrease in endogenous BRs. Comparing transcriptome analyses of the GmDWF1a mutant and wild-type plants revealed a significant impact on the expression of many genes that influence soybean growth. Identifying the GmDWF1a and GmDWF1b genes could aid in the development of compact, densely planted soybean varieties, potentially boosting productivity.
