摘要：
Chitosan (CTS) exhibits notable moisturizing and film-forming capabilities, whereas nano selenium (SeNPs) demonstrates antioxidant and antibacterial properties. In the present study, a CTS/Se film-forming agent was prepared by dissolving CTS, glycerol, and SeNPs in 1% (v/v) glacial acetic acid through heating and ultrasonic treatment. The results revealed that SeNPs fused with CTS via intermolecular forces, thereby enhancing the tensile strength of the CTS film. The water contact angle of the CTS film increased with an increase in SeNPs concentration. The thermal stability and water retention properties of CTS/Se were superior than CTS. In a storage experiment, the content of soluble solids in tomatoes coated with CTS/Se was higher than that in tomatoes coated with CTS and the control. The CTS/Se coating inhibited gas exchange both inside and outside the film, leading to increased activities of antioxidant enzymes. This reduced the oxidation of vitamin C in the tomatoes and decreased the content of malondialdehyde. The CTS/Se film inhibited the growth of bacteria on the tomato surface and mitigated the decline of aldehyde, alcohol and ketone aroma compounds. Consequently, the CTS/Se coating alleviated the softening, aging and rotting of tomatoes. Feeding experiments conducted on mice verified the food safety of the CTS/Se.
Chitosan (CTS) exhibits notable moisturizing and film-forming capabilities, whereas nano selenium (SeNPs) demonstrates antioxidant and antibacterial properties. In the present study, a CTS/Se film-forming agent was prepared by dissolving CTS, glycerol, and SeNPs in 1% (v/v) glacial acetic acid through heating and ultrasonic treatment. The results revealed that SeNPs fused with CTS via intermolecular forces, thereby enhancing the tensile strength of the CTS film. The water contact angle of the CTS film increased with an increase in SeNPs concentration. The thermal stability and water retention properties of CTS/Se were superior than CTS. In a storage experiment, the content of soluble solids in tomatoes coated with CTS/Se was higher than that in tomatoes coated with CTS and the control. The CTS/Se coating inhibited gas exchange both inside and outside the film, leading to increased activities of antioxidant enzymes. This reduced the oxidation of vitamin C in the tomatoes and decreased the content of malondialdehyde. The CTS/Se film inhibited the growth of bacteria on the tomato surface and mitigated the decline of aldehyde, alcohol and ketone aroma compounds. Consequently, the CTS/Se coating alleviated the softening, aging and rotting of tomatoes. Feeding experiments conducted on mice verified the food safety of the CTS/Se.
作者机构：
[Xie, Min; Lai, Yan; Fei, Dan; Zhou, Yaomin; Zhou, YM; Guang, Yelan] Jiangxi Acad Agr Sci, Inst Qual Safety & Stand Agr Prod, Key Lab Agroprod Qual & Safety Jiangxi Prov, Nanchang 330200, Jiangxi, Peoples R China.;[Xu, Jun] Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Med, Nanchang 330200, Jiangxi, Peoples R China.;[Wu, Na] Jiangxi Agr Univ, Coll Food Sci & Technol, Nanchang 330045, Jiangxi, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhou, YM ] J;Jiangxi Acad Agr Sci, Inst Qual Safety & Stand Agr Prod, Key Lab Agroprod Qual & Safety Jiangxi Prov, Nanchang 330200, Jiangxi, Peoples R China.
关键词：
Egg quality;Volatile organic compounds;Chemometric analysis;Odor activity value
摘要：
This study analyzed the egg quality of two commercial layers and two local breeds and detected volatile organic compounds (VOCs) using headspace solid-phase microextraction (HS-SPME-GC-MS) coupled with principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA). A total of 70 VOCs were identified in four types of eggs, mainly including aldehydes, alcohols, ketones, and esters. Chemometric and odor active value (OAV) analysis indicated that cis-3-hexen-1-ol, nonanal, n-decanal, benzaldehyde, and geranial were the main contributing components of flavor in eggs and can also serve as potential marker to distinguish different types of eggs. Both native eggs and commercial eggs have plentiful VOCs. This study contributed to a better understanding of the differences between commercial eggs and native eggs, laying a foundation for the development and utilization of native eggs.
期刊：
CURRENT MEDICINAL CHEMISTRY,2025年32:1-13 ISSN：0929-8673
作者机构：
[Wenbin Liu] College of Medicine and Health Science, Wuhan Polytechnic
University, Wuhan, 430023, China;[Muhammad Umer] Research Center of Forest
Ecology, Institute for Forest Resources and Environment of Guizhou and Forestry College, Guizhou University,
Guiyang, 550025, China;[Yang Xu] Wuhan Polytechnic University School of Modern Industry for Selenium Science and Engineering Wuhan China;[Jixin Chen; Yi He; Shiya Wei; Zhangqian Wang; Chao Gao] National R&[Jixin Chen; Yi He; Shiya Wei; Zhangqian Wang; Chao Gao] D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center
for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science
and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
摘要：
Introduction: G-quadruplexes (G4s) are non-classical high-level structures that are formed by DNA/RNA sequences and have been a promising target for developing antitumor drugs. However, it is still a challenge to find a ligand that binds to a particular G4 with selectivity. Telomeric multimeric G4s are more accessible for screening for specific ligands due to their higher-order structure compared with telomeric monomeric G4s.<&wdkj&>Methods : In this study, the natural product berberine was found to exhibit a higher selectivity for telomeric multimeric G4 in comparison with other G4s. The mechanism of interaction between telomeric G4s and berberine was further investigated by fluorescence spectra measurements, job plot analysis, and UV titrations. We found that there are three binding sites for berberine on telomeric dimeric G-quadruplex Tel45, which are located at the 5' and 3' terminal G-quartet surfaces and the pocket between the two quadruplex units of Tel45. It was worth noting that the berberine preferred to interact within the interfacial cavity between two G4 units.<&wdkj&>Results : Moreover, via dynamic light scattering (DLS) and native polyacrylamide gel electrophoresis (Native-PAGE) assays, it was found that the particle size of the telomeric multimeric G4s conformation was significantly increased by the addition of berberine. In contrast, the particle sizes of Tel21 did not change significantly after the addition of berberine. An immunofluorescence assay indicated that berberine induced the formation of endogenous telomeric G4 structures along with the related telomeric DNA damage response.<&wdkj&>Conclusion: This study provides a hypothetical basis for the development of natural products targeting telomeric G4 as antitumor drugs.
摘要：
Glycyrrhizae Radix et Rhizoma (Gancao) is a functional food whose quality varies significantly owing to the genetic and geographical factors, and is often used by unscrupulous merchants as a substitute for profit. This study developed a rapid identification method for Gancao that determined the species, geographical origin, and main quality marker using a six-channel visual array sensor. This sensor detected the color changes resulting from the competitive coordination with metal ions and color-changing organic dyes. The combination of the array sensor with a random forest (RF) algorithm achieved 100% accuracy in identifying Gancao. Additionally, the smartphone app enabled the rapid and intelligent identification of Gancao within 1 min. The identification accuracy was 91.43%, and the prediction error of two quality markers was less than 13%. This research provides a foundation for rapid and reliable assessment of Gancao quality, enabling the industry to combat fraudulent practices effectively.
Glycyrrhizae Radix et Rhizoma (Gancao) is a functional food whose quality varies significantly owing to the genetic and geographical factors, and is often used by unscrupulous merchants as a substitute for profit. This study developed a rapid identification method for Gancao that determined the species, geographical origin, and main quality marker using a six-channel visual array sensor. This sensor detected the color changes resulting from the competitive coordination with metal ions and color-changing organic dyes. The combination of the array sensor with a random forest (RF) algorithm achieved 100% accuracy in identifying Gancao. Additionally, the smartphone app enabled the rapid and intelligent identification of Gancao within 1 min. The identification accuracy was 91.43%, and the prediction error of two quality markers was less than 13%. This research provides a foundation for rapid and reliable assessment of Gancao quality, enabling the industry to combat fraudulent practices effectively.
期刊：
International Journal of Biological Macromolecules,2025年293:139340 ISSN：0141-8130
通讯作者：
Zhang, Rui;He, JR
作者机构：
[Chen, Ming; Pei, Xun; Yin, Jinjing; Zhang, Rui; Xiong, Sihui; Wu, Muci; He, Jingren] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Oliveira, Helder; Mateus, Nuno] Univ Porto, Fac Sci, Dept Chem & Biochem, REQUIMTE LAQV, P-4169007 Porto, Portugal.;[Ye, Shuxin] Yun Hong Grp Co Ltd, Wuhan 430206, Hubei, Peoples R China.;[Zhang, Rui; He, Jingren] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[He, Jingren; Zhang, Rui] Wuhan Polytech Univ, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.
通讯机构：
[He, JR ; Zhang, R] W;Wuhan Polytech Univ, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.
关键词：
Antioxidant activity;Antiproliferative capacity;Phenolics;Protein;Purple rice (Oryza sativa L.)
摘要：
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
期刊：
International Journal of Biological Macromolecules,2025年314:144319 ISSN：0141-8130
通讯作者：
Sui, Yong;Mei, Xin
作者机构：
[Gao, Xiaomei; Xiong, Tian; Cai, Fang; Wen, Junren; Shi, Jianbin] Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China;[Wen, Junren] College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;[Sui, Yong] Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address: suiyong0429@foxmail.com;[Gao, Xiaomei] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, Wuhan 430023, China;[Mei, Xin] Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address: liangshijiagong@126.com
通讯机构：
[Sui, Yong; Mei, Xin] K;Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address:
摘要：
In this study, the changes in functional properties and binding mechanisms at the molecular level of a non-covalent complex formed between sweet potato leaf polyphenols (SPLPs) and rice bran albumin (RBA) were investigated. Multi-spectral analysis indicated that SPLPs statically quenched the intrinsic fluorescence of RBA, and fitting to a double logarithmic equation revealed that hydrogen bonding constituted the primary driving force behind this interaction. Consequently, the conformational structure, microenvironment, and surface hydrophobicity of RBA were significantly impacted. With 7 μmol/L of SPLPs added to RBA, the emulsifying activity and stability of the complexes were enhanced by 45.71 % and 392.30 %, respectively, compared to RBA. Similarly, the thermal stability of 3,5-diCQA was enhanced by 176.29 %, alongside an improved ultraviolet tolerance. Molecular docking and molecular dynamics simulations clarified that the A0A191ANP5, B8AHL6 and P52428 subunit in RBA has a stronger affinity with the most abundant polyphenols in SPLPs, which was the 3,5-disubstituted caffeoylquinic acid (3,5-diCQA). These findings may furnish a theoretical foundation for the prospective utilization of SPLPs and RBA complex products as functional food ingredients.
In this study, the changes in functional properties and binding mechanisms at the molecular level of a non-covalent complex formed between sweet potato leaf polyphenols (SPLPs) and rice bran albumin (RBA) were investigated. Multi-spectral analysis indicated that SPLPs statically quenched the intrinsic fluorescence of RBA, and fitting to a double logarithmic equation revealed that hydrogen bonding constituted the primary driving force behind this interaction. Consequently, the conformational structure, microenvironment, and surface hydrophobicity of RBA were significantly impacted. With 7 μmol/L of SPLPs added to RBA, the emulsifying activity and stability of the complexes were enhanced by 45.71 % and 392.30 %, respectively, compared to RBA. Similarly, the thermal stability of 3,5-diCQA was enhanced by 176.29 %, alongside an improved ultraviolet tolerance. Molecular docking and molecular dynamics simulations clarified that the A0A191ANP5, B8AHL6 and P52428 subunit in RBA has a stronger affinity with the most abundant polyphenols in SPLPs, which was the 3,5-disubstituted caffeoylquinic acid (3,5-diCQA). These findings may furnish a theoretical foundation for the prospective utilization of SPLPs and RBA complex products as functional food ingredients.
摘要：
Acid-catalyzed organosolv pretreatments using various acids and organic solvents have been widely studied for biomass fractionation. However, few studies have explored whether specific combinations of acids and solvents are necessary to achieve optimal enzymatic cellulose hydrolysis. In this study, organosolv pretreatments were performed on corn stover under mild conditions (120 °C, 2 h) using four biomass-derived solvents (ethylene glycol (EG), 1,4-butanediol (BDO), dimethyl isosorbide (DMI), and γ-valerolactone (GVL)) in an 80:20 solvent-to-water weight ratio, combined with four acids (HCl, H 2 SO 4 , AlCl 3 and p-toluenesulfonic acid (TsOH)) as catalysts (0.1 mol/L). The results showed specific interactions between the acid and solvent. HCl- and AlCl 3 -catalyzed GVL/H 2 O, HCl- and TsOH-catalyzed EG/H 2 O, and HCl-catalyzed DMI/H 2 O exhibited high pretreatment efficacy, achieving enzymatic glucose yields of approximately 80 % after 48 h of hydrolysis. The evaluation of solvent effects using Hansen Solubility Parameters (HSP) revealed no clear correlation with delignification, likely due to the influence of acidic catalysts and the formation of condensed lignin and pseudo-lignin, which may distort the delignification data. Additionally, the chemical composition and cellulose-related factors (accessibility, degree of polymerization and crystallinity) of pretreated biomass were analyzed and correlated with enzymatic glucose yield to evaluate their effects on biomass saccharification. In summary, this study underscores the specificity of acids in organosolv biomass pretreatment and cautions against relying solely on HSP theory for solvent selection when using acidic catalysts.
Acid-catalyzed organosolv pretreatments using various acids and organic solvents have been widely studied for biomass fractionation. However, few studies have explored whether specific combinations of acids and solvents are necessary to achieve optimal enzymatic cellulose hydrolysis. In this study, organosolv pretreatments were performed on corn stover under mild conditions (120 °C, 2 h) using four biomass-derived solvents (ethylene glycol (EG), 1,4-butanediol (BDO), dimethyl isosorbide (DMI), and γ-valerolactone (GVL)) in an 80:20 solvent-to-water weight ratio, combined with four acids (HCl, H 2 SO 4 , AlCl 3 and p-toluenesulfonic acid (TsOH)) as catalysts (0.1 mol/L). The results showed specific interactions between the acid and solvent. HCl- and AlCl 3 -catalyzed GVL/H 2 O, HCl- and TsOH-catalyzed EG/H 2 O, and HCl-catalyzed DMI/H 2 O exhibited high pretreatment efficacy, achieving enzymatic glucose yields of approximately 80 % after 48 h of hydrolysis. The evaluation of solvent effects using Hansen Solubility Parameters (HSP) revealed no clear correlation with delignification, likely due to the influence of acidic catalysts and the formation of condensed lignin and pseudo-lignin, which may distort the delignification data. Additionally, the chemical composition and cellulose-related factors (accessibility, degree of polymerization and crystallinity) of pretreated biomass were analyzed and correlated with enzymatic glucose yield to evaluate their effects on biomass saccharification. In summary, this study underscores the specificity of acids in organosolv biomass pretreatment and cautions against relying solely on HSP theory for solvent selection when using acidic catalysts.
作者机构：
[Liu, Yulan; Wang, Dan; Li, Shunkang; Wu, Nianbang; He, Wensheng; Kuang, Yanling; Zhu, Huiling] Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China;[Gao, Qingyu; Cong, Xin] Enshi Se-Run Material Engineering Technology Co., Ltd., Enshi, China;[Liu, Liping] Beijng Center for Disease Prevention and Control, Beijing, China;[Cheng, Shuiyuan] National R&D Center for Se-rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, China
通讯机构：
[Xin Cong] E;[Dan Wang] H;Enshi Se-Run Material Engineering Technology Co., Ltd., Enshi, China<&wdkj&>Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
摘要：
This study aimed to investigate the impact of Cardamine violifolia on muscle protein degradation, the inflammatory response and antioxidant function in weaned piglets following LPS challenge. Twenty-four weaned piglets were used in a 2 × 2 factorial experiment with dietary treatment (sodium selenite or Cardamine violifolia) and LPS challenge. After 28 days of feeding, pigs were injected intraperitoneally with 100 μg/kg LPS or saline. Dietary supplementation with Cardamine violifolia mitigated the reduction in insulin and growth hormone levels induced by LPS. It also curbed the LPS-induced elevation of plasma glucagon, urea nitrogen, and creatinine concentrations. Cardamine violifolia reduced muscle damage caused by LPS, as evidenced by increased protein content and protein/DNA ratio and decreased TNF-α and IL-1β mRNA expression. Furthermore, Cardamine violifolia modulated the expression of FOXO1, FOXO4, and MuRF1 in muscle, indicative of the protective effect against muscle protein degradation. Enhanced muscle antioxidant function was observed in the form of increased T-AOC, reduced MDA concentration, and decreased mRNA expression of GPX3, DIO3, TXNRD1, SELENOS, SELENOI, SELENOO, and SEPHS2 in LPS-treated piglets. The findings suggest that Cardamine violifolia supplementation can effectively alleviate muscle protein degradation induced by LPS and enhance the antioxidant capacity in piglets.
期刊：
International Journal of Biological Macromolecules,2025年308(Pt 3):142352 ISSN：0141-8130
通讯作者：
Cai, J
作者机构：
[Cai, Jie; Liu, Nian; Yang, Zhaoxing; He, Zhijun; Feng, Xiaofang; Xie, Fang] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Liu, Xiaoqing] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Delivery;OSA starch;Selenium nanoparticles
摘要：
Selenium nanoparticles (SeNPs) exhibit significant potential in antitumor therapy. However, challenges such as aggregation and lack of targeting capability limit their application. Herein, we developed selenium-loaded octenyl succinic anhydride starch (OSAS) micelles functionalized with folic acid (FA) for targeted tumor delivery. The FA-OSAS-SeNPs were synthesized through self-assembly, incorporating SeNPs into FA-conjugated OSAS micelles. Fourier Transform Infrared (FTIR) spectroscopy and UV–visible spectrophotometry confirmed the successful synthesis of FA-OSAS-SeNPs. The nanoparticles exhibited an average size of 131.66 ± 7.88 nm and a zeta potential of −19.54 ± 0.33 mV, with encapsulation efficiency and drug loading capacity of approximately 87.28 % and 8.96 %, respectively. FA-OSAS-SeNPs demonstrated good stability across various conditions, including different dilution ratios, temperatures, pH levels, and ionic strengths. In vitro studies showed that FA-OSAS-SeNPs exhibited significant targeted inhibitory effects on cervical cancer (HeLa) cells and markedly increased intracellular ROS levels, inducing apoptosis. This study presents a novel and effective strategy for targeted SeNPs delivery systems in tumor therapy, offering a valuable reference for future development of nanomaterials for clinical applications in cancer treatment.
Selenium nanoparticles (SeNPs) exhibit significant potential in antitumor therapy. However, challenges such as aggregation and lack of targeting capability limit their application. Herein, we developed selenium-loaded octenyl succinic anhydride starch (OSAS) micelles functionalized with folic acid (FA) for targeted tumor delivery. The FA-OSAS-SeNPs were synthesized through self-assembly, incorporating SeNPs into FA-conjugated OSAS micelles. Fourier Transform Infrared (FTIR) spectroscopy and UV–visible spectrophotometry confirmed the successful synthesis of FA-OSAS-SeNPs. The nanoparticles exhibited an average size of 131.66 ± 7.88 nm and a zeta potential of −19.54 ± 0.33 mV, with encapsulation efficiency and drug loading capacity of approximately 87.28 % and 8.96 %, respectively. FA-OSAS-SeNPs demonstrated good stability across various conditions, including different dilution ratios, temperatures, pH levels, and ionic strengths. In vitro studies showed that FA-OSAS-SeNPs exhibited significant targeted inhibitory effects on cervical cancer (HeLa) cells and markedly increased intracellular ROS levels, inducing apoptosis. This study presents a novel and effective strategy for targeted SeNPs delivery systems in tumor therapy, offering a valuable reference for future development of nanomaterials for clinical applications in cancer treatment.
摘要：
This study explores selenium enrichment of milk through microbial transformation, utilizing Geotrichum candidum LG-8, isolated from traditional dairy products. A combination of electronic tongue, electronic nose, HPLC-ICP-MS, and UHPLC-QTOF-MS were used to evaluate milk quality, organic selenium types, and various components. The milk retained a weakly acidic pH post-transformation, with a flavor profile rich in sour, sweet, and umami tastes. Odor analysis revealed predominant contributions from nitrogen oxides, aromatic compounds, ketones, and organic sulfides. Selenium concentrations increased with higher initial selenium levels, with 50 μg/mL yielding concentrations of MeSeCys, SeCys2, Se(IV), SeMet, and Se(VI) at 5.15, 20.80, 1.41, 75.31 and 0.44 μg/L respectively. Significant changes were observed in the milk’s amino acid profile, with metabolite identification and metabolic pathways mapping(including KEGG). The findings highlight the potential of Geotrichum candidum LG-8 for generating functional organic selenium-enriched milk, offering promising nutritional benefits in dairy production.
This study explores selenium enrichment of milk through microbial transformation, utilizing Geotrichum candidum LG-8, isolated from traditional dairy products. A combination of electronic tongue, electronic nose, HPLC-ICP-MS, and UHPLC-QTOF-MS were used to evaluate milk quality, organic selenium types, and various components. The milk retained a weakly acidic pH post-transformation, with a flavor profile rich in sour, sweet, and umami tastes. Odor analysis revealed predominant contributions from nitrogen oxides, aromatic compounds, ketones, and organic sulfides. Selenium concentrations increased with higher initial selenium levels, with 50 μg/mL yielding concentrations of MeSeCys, SeCys2, Se(IV), SeMet, and Se(VI) at 5.15, 20.80, 1.41, 75.31 and 0.44 μg/L respectively. Significant changes were observed in the milk’s amino acid profile, with metabolite identification and metabolic pathways mapping(including KEGG). The findings highlight the potential of Geotrichum candidum LG-8 for generating functional organic selenium-enriched milk, offering promising nutritional benefits in dairy production.
摘要：
With rising living standards, the demand for health and nutrition has increased, sparking interest in food antioxidants. Known for neutralizing free radicals, antioxidants protect cells from oxidative damage, potentially aiding in disease prevention and anti-aging. In the food industry, they also enhance preservation and quality. Thus, studying food antioxidant mechanisms, detection methods, and applications holds theoretical and practical value. This review mainly discusses the mechanisms, detection methods, and applications of food antioxidants in nutrition. Firstly, the main research status and development trends of food antioxidants are described. Then, the action mechanisms of food antioxidants are introduced. Food antioxidants can effectively remove free radicals and prevent free radicals from causing damage to human cells, thus delaying aging and preventing disease. Secondly, the methods of detecting food antioxidants are discussed, including liquid chromatography, liquid chromatography-tandem mass spectrometry, gas chromatography, and gas chromatography-mass spectrometry. These methods can be used to analyze antioxidant components in various samples of foods, drugs, plants, etc. Finally, the research progress of plant antioxidants is discussed, including the applications of a variety of highly effective antioxidant components extracted from different plants. This review provides the theoretical basis and application reference for the research of food antioxidants.
摘要：
The mortality rate of tumor is still very high till now. Circulating tumor cells (CTCs) are the major culprit of high cancer mortality. To improve survival rate of cancer patients, real-time monitoring and quantitative detection of CTCs are of indescribable value. However, due to the extremely small content and heterogeneous characteristics of CTCs, to accurately detect CTCs is still a tremendous challenge in clinical trials. Herein, the photoelectrochemical aptasensing and fluorescence imaging were co-joint to detect MCF-7 cells in whole blood via an inertial separation microfluidic chip. A portable inertial microfluidic chip with a height of 150 μm was designed to separate MCF-7 cells from whole blood samples. Niblue-C6-IMC was used to label and image MCF-7 cells through specifically reacting with COX-2 enzyme from the over-expression on the cellular surface. Subsequently, MCF-7 cells were detected with a Bi 2 O 2 S nanoflower based photoelectrochemical sensing system. Parameters including the channel height and length, flow rate, focusing position, fluorescence probe concentration, and flow length of the microfluidic chip were optimized. The separation efficiency and purity of MCF-7 cells were 85 % and 80 %, respectively. For 1 mL of blood sample (100 cells mL −1 ), 85 cells and 90 cells could be calculated based on the signals from fluorescence imaging and photoelectrochemical response, respectively. Nevertheless, for assaying 1 mL of blood samples containing 50 MCF-7 cells, 40 cells and 39 cells were obtained from fluorescence imaging and photoelectrochemical responses, respectively.
The mortality rate of tumor is still very high till now. Circulating tumor cells (CTCs) are the major culprit of high cancer mortality. To improve survival rate of cancer patients, real-time monitoring and quantitative detection of CTCs are of indescribable value. However, due to the extremely small content and heterogeneous characteristics of CTCs, to accurately detect CTCs is still a tremendous challenge in clinical trials. Herein, the photoelectrochemical aptasensing and fluorescence imaging were co-joint to detect MCF-7 cells in whole blood via an inertial separation microfluidic chip. A portable inertial microfluidic chip with a height of 150 μm was designed to separate MCF-7 cells from whole blood samples. Niblue-C6-IMC was used to label and image MCF-7 cells through specifically reacting with COX-2 enzyme from the over-expression on the cellular surface. Subsequently, MCF-7 cells were detected with a Bi 2 O 2 S nanoflower based photoelectrochemical sensing system. Parameters including the channel height and length, flow rate, focusing position, fluorescence probe concentration, and flow length of the microfluidic chip were optimized. The separation efficiency and purity of MCF-7 cells were 85 % and 80 %, respectively. For 1 mL of blood sample (100 cells mL −1 ), 85 cells and 90 cells could be calculated based on the signals from fluorescence imaging and photoelectrochemical response, respectively. Nevertheless, for assaying 1 mL of blood samples containing 50 MCF-7 cells, 40 cells and 39 cells were obtained from fluorescence imaging and photoelectrochemical responses, respectively.
摘要：
This study introduces a starch-based composite film integrated with quaternary ammonium chitosan (QCS) and Lycium ruthenicum anthocyanins (LRA) via a facile casting method, designed for intelligent food packaging. The influences of varying concentrations of QCS and/or LRA on water sensitivity, mechanical attributes, UV transmittance, antioxidant capacity, antibacterial performance, and pH-responsive characteristics were meticulously examined. The optimized film demonstrated a tensile strength of ∼0.68 MPa, a contact angle of ∼123.69°, and ABTS radical scavenging efficiency exceeding 80 %. The film exhibited pH-responsive color changes from pink to green across a pH range of 2–12, alongside excellent UV-blocking and antibacterial properties. Shrimp preservation experiments revealed a 16-h shelf-life extension, coupled with real-time freshness monitoring. These findings put forward an exceedingly promising approach to the promotion of starch-based films boasting diverse functionalities in the realm of intelligent food packaging, signifying a stride in this domain.
This study introduces a starch-based composite film integrated with quaternary ammonium chitosan (QCS) and Lycium ruthenicum anthocyanins (LRA) via a facile casting method, designed for intelligent food packaging. The influences of varying concentrations of QCS and/or LRA on water sensitivity, mechanical attributes, UV transmittance, antioxidant capacity, antibacterial performance, and pH-responsive characteristics were meticulously examined. The optimized film demonstrated a tensile strength of ∼0.68 MPa, a contact angle of ∼123.69°, and ABTS radical scavenging efficiency exceeding 80 %. The film exhibited pH-responsive color changes from pink to green across a pH range of 2–12, alongside excellent UV-blocking and antibacterial properties. Shrimp preservation experiments revealed a 16-h shelf-life extension, coupled with real-time freshness monitoring. These findings put forward an exceedingly promising approach to the promotion of starch-based films boasting diverse functionalities in the realm of intelligent food packaging, signifying a stride in this domain.
期刊：
International Journal of Biological Macromolecules,2025年287:138595 ISSN：0141-8130
通讯作者：
Wu, Q
作者机构：
[Ma, Shuzhen; Wang, Jingyi; Zhang, Xue; Wu, Qian; Tao, Sihong] Hubei Univ Technol, Cooperat Innovat Ctr Ind Fermentat, Minist Educ & Hubei Prov, Wuhan 430068, Peoples R China.;[Ma, Shuzhen; Wang, Jingyi; Zhang, Xue; Wu, Qian; Tao, Sihong] Hubei Univ Technol, Sch Life & Hlth Sci, Wuhan 430068, Peoples R China.;[Wu, Muci] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.
通讯机构：
[Wu, Q ] H;Hubei Univ Technol, Sch Life & Hlth Sci, Wuhan 430068, Peoples R China.
关键词：
Casein;Protein-polysaccharide complex coacervates;Low frequency-static magnetic field treatment
摘要：
To evaluate the combination effects of highland barley polysaccharides (HBP) and low-frequency static magnetic field (LF-SMF) treatment on the structure and properties of acid-induced casein (CS) coacervates, this study conducted a comprehensive investigation at various stages- before, during, and after coacervation-for the first time. Compared with native CS, adding HBP caused CS to denature owing to hydrophobic and electrostatic interactions, and LF-SMF treatment further promoted these changes. During the acidification (pH 7.0–2.0) and coacervation processes, the integration of LF-SMF treatment with the addition of HBP enhanced the rate and extent of casein (CS) aggregation and crosslinking, attributable to alterations in the ζ-potential of CS. Among the formed coacervates, the yield and particle size of CS/HBP complex coacervates after being treated with LF-SMF (M-CS/HBP) increased from 67.9 % to 78.4 % and from 803.12 nm to 1253.43 nm, respectively. Additionally, M-CS/HBP demonstrated improved viscoelasticity and a more uniform, compact microstructure with denser packing. The alterations observed in CS-based coacervates were due to non-covalent interactions between CS and HBP, further promoted by LF-SMF treatment, leading to the unfolding and disordering of protein secondary structure. Consequently, M-CS/HBP complex coacervates demonstrated superior encapsulation efficiency for L. plantarum and provided enhanced protection for probiotics under adverse environments.
To evaluate the combination effects of highland barley polysaccharides (HBP) and low-frequency static magnetic field (LF-SMF) treatment on the structure and properties of acid-induced casein (CS) coacervates, this study conducted a comprehensive investigation at various stages- before, during, and after coacervation-for the first time. Compared with native CS, adding HBP caused CS to denature owing to hydrophobic and electrostatic interactions, and LF-SMF treatment further promoted these changes. During the acidification (pH 7.0–2.0) and coacervation processes, the integration of LF-SMF treatment with the addition of HBP enhanced the rate and extent of casein (CS) aggregation and crosslinking, attributable to alterations in the ζ-potential of CS. Among the formed coacervates, the yield and particle size of CS/HBP complex coacervates after being treated with LF-SMF (M-CS/HBP) increased from 67.9 % to 78.4 % and from 803.12 nm to 1253.43 nm, respectively. Additionally, M-CS/HBP demonstrated improved viscoelasticity and a more uniform, compact microstructure with denser packing. The alterations observed in CS-based coacervates were due to non-covalent interactions between CS and HBP, further promoted by LF-SMF treatment, leading to the unfolding and disordering of protein secondary structure. Consequently, M-CS/HBP complex coacervates demonstrated superior encapsulation efficiency for L. plantarum and provided enhanced protection for probiotics under adverse environments.
摘要：
In this study, we extracted, separated, and purified polysaccharides from Se-enriched Cyclocarya paliurus (Se-CPP-1) and compared them with their non-Se-enriched counterparts (CPP-1) to investigate the impact of selenium on their structural and functional properties. Structural characterization by HPLC, GC-MS, and SEM revealed that Se-CPP-1 is an acidic heteropolysaccharide with a lower molecular weight (76.6 vs. 109.22 kDa), smaller particle size (418.22 vs. 536.96 nm), and higher negative zeta potential (-43.15 vs. -21.29 mV), indicating enhanced colloidal stability. SEM imaging further demonstrated a distinctive flaky morphology in Se-CPP-1. Functional assays showed that Se-CPP-1 significantly outperformed CPP-1 in scavenging free radicals (DPPH/ABTS), stimulating RAW264.7 macrophage proliferation (CCK-8 assay), enhancing phagocytic activity, and promoting NO secretion. These improvements were attributed to selenium-induced modifications in polysaccharide conformation and surface properties. Our findings highlight the potential of selenium fortification in developing high-efficacy C. paliurus polysaccharides for antioxidant and immunomodulatory applications.
期刊：
FRONTIERS IN ONCOLOGY,2025年14:1490740 ISSN：2234-943X
作者机构：
[He, Lingwen; Zhang, Lu] Department of Oncology, Dongguan Songshan Lake Tungwah Hospital, Dongguan, China;[Peng, Yulong] Department of Oncology, Dongguan Tungwah Hospital, Dongguan, China;[He, Zhijun] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, China;[He, Zhijun] Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, China
摘要：
Selenium (Se) is important and plays significant roles in many biological processes or physiological activities. Prolonged selenium deficiency has been conclusively linked to an elevated risk of various diseases, including but not limited to cancer, cardiovascular disease, inflammatory bowel disease, Keshan disease, and acquired immunodeficiency syndrome. The intricate relationship between selenium status and health outcomes is believed to be characterized by a non-linear U-shaped dose-response curve. This review delves into the significance of maintaining optimal selenium levels and the detrimental effects that can arise from selenium deficiency. Of particular interest is the important role that selenium plays in both prevention and treatment of cancer. Finally, this review also explores the diverse classes of selenium entities, encompassing selenoproteins, selenium compounds and selenium nanoparticles, while examining the mechanisms and molecular targets of their anticancer efficacy.
期刊：
Journal of Analytical and Applied Pyrolysis,2025年:107167 ISSN：0165-2370
通讯作者：
Yingquan Chen<&wdkj&>Mingwei Xia
作者机构：
[Shan Cao; Zhiqiang Chen; Ziyue Tang; Yingquan Chen; Mingwei Xia; Haiping Yang; Hanping Chen] State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, 1037 Luoyu Road, 430074, Wuhan, PR China;[Wei Chen] College of Engineering, Nanjing Agricultural University, 210095, Nanjing, PR China;[Xu Chen] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, 430023, Wuhan, PR China
通讯机构：
[Yingquan Chen; Mingwei Xia] S;State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, 1037 Luoyu Road, 430074, Wuhan, PR China
摘要：
Potassium-assisted biomass pyrolysis is highly efficient for product poly-generation and upgrading. In this study, kinetics modeling of over-saturated potassium (K 2 CO 3 )-assisted lignocellulose pyrolysis process was carried out by using the modified Friedman-based master plot (MF-MP) method combined with multiple-parallel compensation-simplified distributed activation energy model (CS-DAEM). The effects of K 2 CO 3 dosage on the lignocellulose pyrolysis process were also studied. MF-MP results show that all the K 2 CO 3 -assisted pyrolysis processes of cellulose, xylan, and lignin obey the Avrami-Erofeev (Am) nucleation mechanism, where potassium slightly decreases the activation energies. The potassium-assisted pyrolysis process of typical lignocellulose (bamboo) also obeys the Am mechanism, and 3G-Am-CS-DAEM modeling of the processes achieves R 2 >0.999. By fixing the pre-exponential factor, the compensation effect was simplified. Based on modeling results, the activation energy decreases as potassium dosage increases from 0.01 mmol/g to 10 mmol/g. This study provides an effective kinetics approach to characterize the potassium-assisted biomass pyrolysis process.
Potassium-assisted biomass pyrolysis is highly efficient for product poly-generation and upgrading. In this study, kinetics modeling of over-saturated potassium (K 2 CO 3 )-assisted lignocellulose pyrolysis process was carried out by using the modified Friedman-based master plot (MF-MP) method combined with multiple-parallel compensation-simplified distributed activation energy model (CS-DAEM). The effects of K 2 CO 3 dosage on the lignocellulose pyrolysis process were also studied. MF-MP results show that all the K 2 CO 3 -assisted pyrolysis processes of cellulose, xylan, and lignin obey the Avrami-Erofeev (Am) nucleation mechanism, where potassium slightly decreases the activation energies. The potassium-assisted pyrolysis process of typical lignocellulose (bamboo) also obeys the Am mechanism, and 3G-Am-CS-DAEM modeling of the processes achieves R 2 >0.999. By fixing the pre-exponential factor, the compensation effect was simplified. Based on modeling results, the activation energy decreases as potassium dosage increases from 0.01 mmol/g to 10 mmol/g. This study provides an effective kinetics approach to characterize the potassium-assisted biomass pyrolysis process.
摘要：
Encapsulation is a promising strategy to increase the physicochemical stability of anthocyanins (ACNs). In this work, red cabbage anthocyanins-loaded composite nanoparticles were prepared by the self-assembly of zein protein and anionic polysaccharides. Three anionic polysaccharides and two types of preparation approaches were used to form different ACNs-loaded zein/polysaccharide nanoparticles. Results revealed that ACNs-loaded zein/polysaccharide nanoparticles showed spherical morphology with an average particle size in the range of 102.6–360.4 nm, polydispersity index of 0.214–0.415, zeta potential of - (26.7–42.6) mV and the total ACNs encapsulation efficiency (EE) of 68.03–80.55 %, depending on the type of polysaccharides and the different adding sequences of the raw materials. Polysaccharides coating increased the EEs of ACNs as compared to the zein alone. ACNs monomers with acylated groups presented higher EEs. ATR-FTIR spectrum indicated hydrogen bonds, hydrophobic and electrostatic interactions were the dominant forces among ACNs, zein and polysaccharides. In addition, ACNs-loaded zein/polysaccharide nanoparticles showed good colloidal stability to different pH, ionic strength and storage conditions as compared to ACNs-loaded zein nanoparticles due to the electrostatic and steric repulsion from the polysaccharide coating on the nanoparticle surface.
Encapsulation is a promising strategy to increase the physicochemical stability of anthocyanins (ACNs). In this work, red cabbage anthocyanins-loaded composite nanoparticles were prepared by the self-assembly of zein protein and anionic polysaccharides. Three anionic polysaccharides and two types of preparation approaches were used to form different ACNs-loaded zein/polysaccharide nanoparticles. Results revealed that ACNs-loaded zein/polysaccharide nanoparticles showed spherical morphology with an average particle size in the range of 102.6–360.4 nm, polydispersity index of 0.214–0.415, zeta potential of - (26.7–42.6) mV and the total ACNs encapsulation efficiency (EE) of 68.03–80.55 %, depending on the type of polysaccharides and the different adding sequences of the raw materials. Polysaccharides coating increased the EEs of ACNs as compared to the zein alone. ACNs monomers with acylated groups presented higher EEs. ATR-FTIR spectrum indicated hydrogen bonds, hydrophobic and electrostatic interactions were the dominant forces among ACNs, zein and polysaccharides. In addition, ACNs-loaded zein/polysaccharide nanoparticles showed good colloidal stability to different pH, ionic strength and storage conditions as compared to ACNs-loaded zein nanoparticles due to the electrostatic and steric repulsion from the polysaccharide coating on the nanoparticle surface.
摘要：
In this study, the physicochemical properties, in vitro fermentation, and interaction with Bacteroides ovatus of a purified fraction of polysaccharides from the root of Brassica rapa (BRP2–2) were investigated. It was demonstrated with an in vitro anaerobic fermentation model that BRP2–2 significantly increased ( p < 0.05) the relative abundance of Bacteroides spp. After 24 h incubation of BRP2–2 with Bacteroides ovatus alone, 10.24 ± 0.69, 8.76 ± 0.48 and 3.37 ± 0.26 mM of acetate, propionate and isovalerate were produced, respectively. Moreover, transcriptome analysis of B. ovatus showed that 143 genes were up-regulated by BRP2–2, including five discrete polysaccharide utilization loci and two carbohydrate-active enzyme clusters. Based on the annotation of carbohydrate enzyme function, we speculate that BRP2–2 is a pectic polysaccharide containing homogalacturonan, rhamnogalacturonan I and rhamnogalacturonan II domains. These results suggested that BRP2–2 was degraded by B. ovatus , which produced metabolites with beneficial effects on host health.
In this study, the physicochemical properties, in vitro fermentation, and interaction with Bacteroides ovatus of a purified fraction of polysaccharides from the root of Brassica rapa (BRP2–2) were investigated. It was demonstrated with an in vitro anaerobic fermentation model that BRP2–2 significantly increased ( p < 0.05) the relative abundance of Bacteroides spp. After 24 h incubation of BRP2–2 with Bacteroides ovatus alone, 10.24 ± 0.69, 8.76 ± 0.48 and 3.37 ± 0.26 mM of acetate, propionate and isovalerate were produced, respectively. Moreover, transcriptome analysis of B. ovatus showed that 143 genes were up-regulated by BRP2–2, including five discrete polysaccharide utilization loci and two carbohydrate-active enzyme clusters. Based on the annotation of carbohydrate enzyme function, we speculate that BRP2–2 is a pectic polysaccharide containing homogalacturonan, rhamnogalacturonan I and rhamnogalacturonan II domains. These results suggested that BRP2–2 was degraded by B. ovatus , which produced metabolites with beneficial effects on host health.
摘要：
Sausage is favored due to its rich nutrition and good taste, but usually contains high sodium concentration. Additionally, physicochemical changes happening during the cooking process of sausage resulted in water loss and poor flavor. Plant proteins are important ingredients in meat alternatives. This study explored the effect of plant proteins on the physical and flavor properties of reduced-sodium pork sausages. The results showed that soy protein isolate had the highest gel strength, water holding capacity (WHC) and oil holding capacity (OHC), pea protein had higher gel strength, WHC and OHC than peanut protein. Besides, the emulsion activity of soy protein isolate and pea protein was higher than that of peanut protein. KCl substitution minimally affected the color of soy protein isolate sausage and WHC of pea protein sausage. LF-NMR results showed KCl didn't change water distribution of sausages. Electronic tongue analysis revealed enhanced umami and reduced sourness with increased KCl in pea protein sausage. Furthermore, Soy protein isolate sausage with 20% KCl substitution and pea protein sausage with 40% KCl substitution had the highest sensory scores. Overall, soy protein isolate and pea protein are promising ingredients with 20% and 30%–40% KCl substitution in sausage, respectively.
Sausage is favored due to its rich nutrition and good taste, but usually contains high sodium concentration. Additionally, physicochemical changes happening during the cooking process of sausage resulted in water loss and poor flavor. Plant proteins are important ingredients in meat alternatives. This study explored the effect of plant proteins on the physical and flavor properties of reduced-sodium pork sausages. The results showed that soy protein isolate had the highest gel strength, water holding capacity (WHC) and oil holding capacity (OHC), pea protein had higher gel strength, WHC and OHC than peanut protein. Besides, the emulsion activity of soy protein isolate and pea protein was higher than that of peanut protein. KCl substitution minimally affected the color of soy protein isolate sausage and WHC of pea protein sausage. LF-NMR results showed KCl didn't change water distribution of sausages. Electronic tongue analysis revealed enhanced umami and reduced sourness with increased KCl in pea protein sausage. Furthermore, Soy protein isolate sausage with 20% KCl substitution and pea protein sausage with 40% KCl substitution had the highest sensory scores. Overall, soy protein isolate and pea protein are promising ingredients with 20% and 30%–40% KCl substitution in sausage, respectively.
