摘要：
Carboxymethyl chitosan (CMCS) has antibacterial activity and coating-forming ability. Under the impact of noncovalent interactions, the bioactivity and functionality of CMCS may be positively affected by the coexistence of flavonoids. This study investigated the effect of a CMCS coating incorporated with flavonoids from guava (Psidium guajava L. cv. Carmine) leaf (GLF) on the refrigeration of fresh-cut apples for preservation. Compared with the CMCS group, apples treated with the CMCS-GLF coating showed better quality (weight loss, browning index, firmness), nutritional value (ascorbic acid and total phenolic content), and microbial safety during storage. The mechanism study indicated that the hydrogen bonding, electrostatic, and hydrophobic interactions between CMCS and GLF (the carboxymethyl moiety of CMCS had the highest response priority and binding strength of the interaction with -C-O of GLF) changed the surface charge distribution and microstructure of CMCS, and increased its molecular weight, particle size, viscosity, and hydrophobicity. Thus, the CMCS-GLF coating exerted better bioactivities (antibacterial and antioxidant activity), and its film showed better mechanical and barrier properties. These results revealed that the noncovalent interaction with GLF could modify the physiochemical properties of CMCS, which was beneficial to improve its bioactivity and application value in fresh fruit preservation.
摘要：
Advanced glycation end-products (AGEs) are the final products of the non-enzymatic interaction between reducing sugars and amino groups in proteins, lipids and nucleic acids. In numerous diseases, such as diabetes, neuropathy, atherosclerosis, aging, nephropathy, retinopathy, and chronic renal illness, accumulation of AGEs has been proposed as a pathogenic mechanism of inflammation, oxidative stress, and structural tissue damage leading to chronic vascular issues. Current studies on the inhibition of AGEs mainly focused on food processing. However, there are few studies on the inhibition of AGEs during digestion, absorption and metabolism although there are still plenty of AGEs in our body with our daily diet. This review comprehensively expounded AGEs inhibition mechanism based on the whole process of digestion, absorption and metabolism by polyphenols, amino acids, hydrophilic colloid, carnosine and other new anti-glycation agents. Our study will provide a ground-breaking perspective on mediation or inhibition AGEs.
作者：
An, Jiejie;Liu, Mingzhu;Din, Zia-ud;Xie, Fang*;Cai, Jie
期刊：
International Journal of Biological Macromolecules,2023年248:126180 ISSN：0141-8130
通讯作者：
Xie, Fang;Cai, J
作者机构：
[Cai, Jie; An, Jiejie; Liu, Mingzhu; Xie, Fang; Xie, F] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Wuhan 430023, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;[Din, Zia-ud] Women Univ Swabi, Dept Food Sci & Nutr, Swabi 23430, Khyber Pakhtunk, Pakistan.
通讯机构：
[Cai, J ; Xie, F] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Wuhan 430023, Peoples R China.
作者机构：
[Jia, Jilai; Zhou, Jiaojiao; Cai, Jie; Zhu, Weijia; Zhou, JJ; Xie, Fang] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Jia, Jilai; Zhou, Jiaojiao; Cai, Jie; Zhu, Weijia; Zhou, JJ] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[Du, Xiaoping] Minist Agr & Rural Affairs, Key Lab Se Enriched Prod Dev & Qual Control, Ankang R&D Ctr Se Enriched Prod, Ankang 725000, Shaanxi, Peoples R China.;[Din, Zia-ud] Women Univ Swabi, Dept Food Sci & Nutr, Swabi 23430, Khyber Pakhtunk, Pakistan.
通讯机构：
[Cai, J ; Zhou, JJ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
摘要：
Due to their high toxicity and ongoing bioaccumulation, mercury ions (Hg(2+)) can cause significant harm to both the environment and human health. Therefore, rapid, accurate, and selective methods for Hg(2+) detection are highly desirable. Herein, we present a simple method for depositing platinum nanoparticles (PtNPs) on graphene oxide (GO) to obtain graphene oxide-PtNPs (GO-PtNPs). The fabricated GO-PtNPs exhibit excellent peroxidase-like activity and high stability. Further, the GO-PtNPs nanozymes preferentially reduced Hg(2+), thereby inhibiting the catalytic activity. By monitoring the color change in the chromogenic substrate, Hg(2+) can be detected within 15 min. With a detection limit of 88.3 pM, the GO-PtNPs system may be employed to detect Hg(2+) in a linear range of 0.1 nM to 10 μM. The simplicity and low cost of the proposed approach as well as its applicability to complicated samples demonstrate its capacity for mercury sensing in environmental samples.
通讯机构：
[Li Li] S;School of Modern Industry for Selenium Science and Engineering, National R&D Center for Se-Rich Agricultural Products Processing Technology, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Selenium (Se) is an essential element for mammals, and its deficiency in the diet is a global problem. Agronomic biofortification through exogenous Se provides a valuable strategy to enhance human Se intake. Selenium nanoparticles (SeNPs) have been regarded to be higher bioavailability and less toxicity in comparison with selenite and selenate. Still, little has been known about the mechanism of their metabolism in plants. Soybean (Glycine max L.) can enrich Se, providing an ideal carrier for Se biofortification. In this study, soybean sprouts were treated with SeNPs, and a combination of next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing was applied to clarify the underlying molecular mechanism of SeNPs metabolism. A total of 74,662 nonredundant transcripts were obtained, and 2109 transcription factors, 9687 alternative splice events, and 3309 long non-coding RNAs (lncRNAs) were predicted, respectively. KEGG enrichment analysis of the DEGs revealed that metabolic pathways, biosynthesis of secondary metabolites, and peroxisome were most enriched both in roots and leaves after exposure to SeNPs. A total of 117 transcripts were identified to be putatively involved in SeNPs transport and biotransformation in soybean. The top six hub genes and their closely coexpressed Se metabolism-related genes, such as adenylylsulfate reductase (APR3), methionine-tRNA ligase (SYM), and chloroplastic Nifs-like cysteine desulfurases (CNIF1), were screened by WGCNA and identified to play crucial roles in SeNPs accumulation and tolerance in soybean. Finally, a putative metabolism pathway of SeNPs in soybean was proposed. These findings have provided a theoretical foundation for future elucidation of the mechanism of SeNPs metabolism in plants.
摘要：
Sepsis is a life-threatening organ dysfunction caused by the dysregulated response of the host to an infection, and treatments are limited. Recently, a novel selenium source, selenium-enriched Cardamine violifolia (SEC) has attracted much attention due to its anti-inflammatory and antioxidant properties, but little is known about its role in the treatment of sepsis. Here, we found that SEC alleviated LPS-induced intestinal damage, as indicated by improved intestinal morphology, and increased disaccharidase activity and tight junction protein expression. Moreover, SEC ameliorated the LPS-induced release of pro-inflammatory cytokines, as indicated by decreased IL-6 level in the plasma and jejunum. Moreover, SEC improved intestinal antioxidant functions by regulating oxidative stress indicators and selenoproteins. In vitro, TNF-α-challenged IPEC-1 cells were examined and showed that selenium-enriched peptides, which are the main functional components extracted from Cardamine violifolia (CSP), increased cell viability, decreased lactate dehydrogenase activity and improved cell barrier function. Mechanistically, SEC ameliorated LPS/TNF-α-induced perturbations in mitochondrial dynamics in the jejunum and IPEC-1 cells. Moreover, CSP-mediated cell barrier function is primarily dependent on the mitochondrial fusion protein MFN2 but not MFN1. Taken together, these results indicate that SEC mitigates sepsis-induced intestinal injury, which is associated with modulating mitochondrial fusion.
摘要：
Theoretically, lactic acid bacteria (LABs) could degrade polyphenols into small molecular compounds. In this study, the biotransformation of lotus seedpod and litchi pericarp procyanidins by Lactobacillus plantarum 90 (Lp90), Streptococcus thermophilus 81 (ST81), Lactobacillus rhamnosus HN001 (HN001), and Pediococcus pentosus 06 (PP06) were analysed. The growth curve results indicated that procyanidins did not significantly inhibit the proliferation of LABs. Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS) revealed that procyanidin B2 and procyanidin B3 in lotus seedpod decreased by 62.85% and 25.45%, respectively, with ST81 metabolised, while kaempferol and syringetin 3-O-glucoside content increased. Although bioconversion did not increase the inhibitory function of procyanidins against glycosylation end-products in vitro, the 2,2 '-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) free radical scavenging capacity and ferric reducing antioxidant power of litchi pericarp procyanidins increased by 157.34% and 6.8%, respectively, after ST81 biotransformation. These findings may inspire further studies of biological metabolism of other polyphenols and their effects on biological activity.
作者机构：
[Yang, Rundong; Guo, Yuhao; Wang, Feifei] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Wang, Feifei] Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China.;[Zhang, Chengwu] Jinan Univ, Dept Ecol, Guangzhou 510632, Peoples R China.;[Zhang, Chengwu] Jinan Univ, Res Ctr Hydrobiol, Guangzhou 510632, Peoples R China.
通讯机构：
[Chengwu Zhang] D;Department of Ecology and Research Center for Hydrobiology, Jinan University, Guangzhou 510632, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
The aim of this study is to investigate the differences in the accumulation capacity of chrysolaminarin among six Tribonema species and to isolate this polysaccharide for immunomodulatory activity evaluation. The results showed that T. aequale was the most productive strain with the highest content and productivity of chrysolaminarin, which were 17.20% (% of dry weight) and 50.91 mg/L/d, respectively. Chrysolaminarin was then extracted and isolated from this alga, and its monosaccharide composition was mainly composed of a glucose (61.39%), linked by beta-D-(1 -> 3) (main chain) and beta-D-(1 -> 6) (branch chain) glycosidic bonds, with a molecular weight of less than 6 kDa. In vitro immunomodulatory assays showed that it could activate RAW264.7 cells at a certain concentration (1000 mu g/mL), as evidenced by the increased phagocytic activity and upregulated mRNA expression levels of IL-1 beta, IL6, TNF-alpha and Nos2. Moreover, Western blot revealed that this polysaccharide stimulated the phosphorylation of p-65, p-38 and JNK in NF-kappa B and MAPK signaling pathways. Overall, these findings provide a reference for the further development and utilization of algae-based chrysolaminarin, while also offering an in-depth understanding of the immunoregulatory mechanism.
作者机构：
[He, Yi; Shao, Yanchun; Zhu, Lisha; Zhang, Hao; He, Jingren] Wuhan Polytech Univ, Sch Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Key Lab Deep Proc Major Grain & Oil,Minist Educ, Wuhan 430023, Peoples R China.;[He, Yi; He, Y; He, Jingren] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Shao, Yanchun] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Wang, Liling] Tarim Univ, Coll Life Sci, Alar 843300, Peoples R China.
通讯机构：
[He, Y ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Monascus red pigments;Microcapsules;Process optimization;Morphological characteristics;Stability analysis
摘要：
Monascus red pigments (MRPs), natural food colorants from microorganisms, are widely used in the food industry but are not very stable during storage and processing. Therefore, this study aims to microencapsulate MRPs using an emulsification/internal gelation technique to improve the stability. The highest encapsulation efficiency (EE) of 70.32% +/- 0.28% was achieved when the percentage of NaCl in phosphate buffered saline (PBS) was 0.88%, the mass ratio of CaCO3 to NaAlg was 3.13, and the molar ratio of calcium to acid was 4.15. Spray-dried MRPs microcapsules had a smaller average diameter (D [4,3] approximate to 3 mu m) with a smooth surface and spherical shape. Moreover, the stability assays revealed that spray-dried MRPs microcapsules processed higher retention rate, especially under the treatments of heating, light and in vitro simulated gastrointestinal digestion. The spray-dried MRPs microcapsules exhibited the lowest degradation constant and longest half-life compared with free MRPs and freeze-dried MRPs microcapsules. These results demonstrated that combining emulsification/internal gelation with spray-drying is feasible for enhancing the stability of MRPs to broad its further application in the food industry.
作者机构：
[Liu, Mingkang; Liu, Yulan; Qin, Kun; Wang, Hui; Yan, Mengke; Zhu, Huiling; Xu, Xiao; Xu, Xianfeng] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Gao, Qingyu; Zhang, Yue; Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.;[Cheng, Shuiyuan] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Zhao, Jiangchao] Univ Arkansas, Dept Anim Sci, Div Agr, Fayetteville, NC 72701 USA.
通讯机构：
[Huiling Zhu; Yulan Liu] A;Authors to whom correspondence should be addressed.<&wdkj&>Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
As a selenium-enriched plant, Cardamine violifolia (SEC) has an excellent antioxidant function. The edibility of SEC is expected to develop new sources of organic Se supplementation for human and animal nutrition. This study was conducted to investigate the effects of SEC on laying performance and ovarian antioxidant capacity in aging laying hens. A total of 450 laying hens were assigned to five treatments. Dietary treatments included the following: a basal diet (diet without Se supplementation, CON) and basal diets supplemented with 0.3 mg/kg Se from sodium selenite (SS), 0.3 mg/kg Se from Se-enriched yeast (SEY), 0.3 mg/kg Se from SEC, or 0.3 mg/kg Se from SEC and 0.3 mg/kg Se from SEY (SEC + SEY). Results showed that supplementation with SEC tended to increase the laying rate, increased the Haugh unit of eggs, and reduced the FCR. SEC promoted ovarian cell proliferation, inhibited apoptosis, and ameliorated the maintenance of follicles. SEC, SEY, or SEC + SEY increased ovarian T-AOC and decreased MDA levels. SEC increased the mRNA abundance of ovarian selenoproteins. SEC and SEC + SEY increased the mRNA abundance of Nrf2, HO-1, and NQO1, and decreased the mRNA abundance of Keap1. These results indicate that SEC could potentially to improve laying performance and egg quality via the enhancement of ovarian antioxidant capacity. SEC exerts an antioxidant function through the modulation of the Nrf2/Keap1 signaling pathway.
摘要：
It is an essential method for healthy Selenium (Se) supplementation to convert exogenous Se into organic Se via crops. Brassica juncea (L.) Czern (leaf mustard) was employed as plant material in this investigation and was treated with sodium selenite (Na2SeO3). Its physiological indicators, nutritional quality, antioxidant enzyme activity, total Se content, and Se morphology were all evaluated. The absorption, transportation, and transformation mechanisms of Se in mustard were studied using transcriptome data. The results revealed that low concentration of Se treatment promoted the growth of mustard, while high concentration Se treatment inhibited it. The concentration of 10 mg/L Na2SeO3 treatment had the best growth parameters for mustard. Compared to the control group, the content of vitamin C (Vc) and anthocyanins in the treatment group increased to varying degrees, while the content of flavonoids, total phenols, soluble sugar, and soluble protein increased first and then decreased. Five Se forms, Se (IV), Se (VI), selenocystine(SeCys2), selenomethionine (SeMet), and methylselenocysteine (MeSeCys), were detected in the Na2SeO3 treatment group, with organic Se accounting for over 95%. Na2SeO3 treatment can significantly reduce the accumulation of ROS in mustard plants and enhance their stress resistance. Transcriptome data and metabolite association analysis showed that PHO1-H8 promoted the absorption of Na2SeO3 by mustard roots, while SULTR3;3 and SULTR4;1 promoted the transport of Se from roots to the aboveground portion and chloroplasts. Se in mustard was transformed into SeMet, SeCys, MeSeCys, and selenoprotein through the action of genes such as APS, APR, and SEP1, and stored in plant leaves.
作者：
Teixeira, Margarida;Tao, Wen;Fernandes, Ana;Faria, Ana;Ferreira, Isabel M. P. L. V. O.;...
期刊：
Trends in Food Science & Technology,2023年138:708-725 ISSN：0924-2244
通讯作者：
Oliveira, HE
作者机构：
[Fernandes, Ana; de Freitas, Victor; Oliveira, H. elder; Teixeira, Margarida; Oliveira, HE; Mateus, Nuno; Tao, Wen] Fac Sci, Dept Chem & Biochem, LAQV, REQUIMTE, P-4169007 Porto, Portugal.;[Faria, Ana] Univ NOVA Lisboa, Fac Ciencias Med, NOVA Med Sch, NMS,FCM,CHRC, Lisbon, Portugal.;[Faria, Ana] Univ NOVA Lisboa, NOVA Med Sch, Nutr & Metab, Fac Ciencias Med,NMS,FCM,CINTESIS RISE, Lisbon, Portugal.;[Ferreira, Isabel M. P. L. V. O.] Univ Porto, Fac Pharm, Dept Chem Sci, LAQV REQUIMTE,Lab Bromatol & Hydrol, P-4050313 Porto, Portugal.;[He, Jingren] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Wuhan 430023, Peoples R China.
摘要：
Background: Among the many sources of anthocyanins, edible flowers are regaining interest for both consumers and researchers due to their nutritional profile and the need for even more healthy dietary alternatives. In such context, anthocyanin-rich edible flowers may be one of the most interesting groups of such cultivars but also of anthocyanins source. Scope and approach: In this review, we discuss the latest findings regarding such type of edible flowers, from their consumption patterns to their nutritional and anthocyanins composition, their reported health benefits, the challenges about the consumption of edible flowers and the future research necessities on this promising thematic. Key findings and conclusions: Anthocyanins have become a key group of natural compounds during the last years due to their broad applications in different areas. From a nutritional and health perspective, these compounds have been showing potential roles against different pathologies. The excellent aroma, taste and appearance of anthocyanin-rich edible flowers turns meals more appealing to consumers. Moreover, their nutritional profile, bioactive properties, and health benefits, encourages the development of functional foods with nutraceutical purposes, thus promoting the consumption of these type of edible flowers worldwide. Further knowledge in food processing methods is a key factor on the comeback and the addition of anthocyanin-rich edible flowers to our dietary habits.
