作者机构：
[He, Jiangling; Zhou, Jiaojiao; Cai, Jie; Xie, Fang; Liu, Yuantao; Gui, Yue] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Du, Xiaoping] Minist Agr & Rural Affairs, Ankang R&D Ctr Se Enriched Prod, Key Lab Se Enriched Prod Dev & Qual Control, Ankang 725000, Shaanxi, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
摘要：
A colorimetric sensor detects an analyte by utilizing the optical properties of the sensor unit, such as absorption or reflection, to generate a structural color that serves as the output signal to detect an analyte. Detecting the refractive index of an analyte by recording the color change of the sensor structure on its surface has several advantages, including simple operation, low cost, suitability for onsite analysis, and real-time detection. Colorimetric sensors have drawn much attention owing to their rapidity, simplicity, high sensitivity and selectivity. This Review discusses the use of colorimetric sensors in the food industry, including their applications for detecting food contaminants. The Review also provides insight into the scope of future research in this area.
期刊：
Journal of Fungi,2023年9(12):1174- ISSN：2309-608X
通讯作者：
He, Y;Shao, YC
作者机构：
[He, Yi; Zhu, Lisha; Dong, Xingxing; He, Y] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Wuhan 430023, Peoples R China.;[He, Yi; Li, Aoran; Zhu, Lisha; He, Y; Xu, Suyin] Wuhan Polytech Univ, Sch Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod,Minist E, Wuhan 430023, Peoples R China.;[Wang, Liling] Tarim Univ, Coll Food Sci & Engn, Alar 843300, Peoples R China.;[Shao, Yanchun; Shao, YC] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.
通讯机构：
[Shao, YC ] H;[He, Y ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Sch Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod,Minist E, Wuhan 430023, Peoples R China.;Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.
关键词：
alternative splicing;citrinin;glycolysis;lipid metabolism;Monascus ruber;RNA interference
摘要：
Citrinin (CIT), a secondary metabolite produced by the filamentous fungi Monascus species, exhibits nephrotoxic, hepatotoxic, and carcinogenic effects in mammals, remarkably restricting the utilization of Monascus-derived products. CIT synthesis is mediated through the pksCT gene and modified by multiple genetic factors. Here, the regulatory effects of two pksCT transcripts, pksCT alpha, and pksCT beta, generated via pre-mRNA alternative splicing (AS), were investigated using hairpin RNA (ihpRNA) interference, and their impact on CIT biosynthesis and the underlying mechanisms were assessed through chemical biology and transcriptome analyses. The CIT yield in ihpRNApksCT alpha and ihpRNA-pksCT (alpha + beta) transformants decreased from 7.2 mu g/mL in the wild-type strain to 3.8 mu g/mL and 0.08 mu g/mL, respectively. Notably, several genes in the CIT biosynthetic gene cluster, specifically mrl3, mrl5, mrr1, and mrr5 in the ihpRNA-pksCT (alpha + beta) transformant, were downregulated. Transcriptome results revealed that silencing pksCT has a great impact on carbohydrate metabolism, amino acid metabolism, lipid metabolism, and AS events. The key enzymes in the citrate cycle (TCA cycle) and glycolysis were significantly inhibited in the transformants, leading to a decrease in the production of biosynthetic precursors, such as acetyl-coenzyme-A (acetyl-coA) and malonyl-coenzyme-A (malonyl-coA). Furthermore, the reduction of CIT has a regulatory effect on lipid metabolism via redirecting acetyl-coA from CIT biosynthesis towards lipid biosynthesis. These findings offer insights into the mechanisms underlying CIT biosynthesis and AS in Monascus, thus providing a foundation for future research.
作者机构：
[Zhang, Junjie; Chen, Hanping; Shao, Jing'ai; Rao, Gang; Fu, Liwen; Zhang, Xiong] Huazhong Univ Sci & Technol, State Key Lab Coal Combust, Wuhan 430074, Peoples R China.;[Chen, Hanping; Wang, Qiancan; Shao, Jing'ai; Rao, Gang] Huazhong Univ Sci & Technol, Sch Energy & Power Engn, Dept New Energy Sci & Technol, Wuhan 430074, Peoples R China.;[Chen, Xu] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Krivoshapkina, Elena; Krivoshapkin, Pavel] ITMO Univ, EnergyLab, St Petersburg 197101, Russia.;[Shao, Jing'ai; Chen, Xu] Huazhong Univ Sci & Technol, State Key Lab Coal Combust, 1037 Luoyu Rd, Wuhan 430074, Hubei, Peoples R China.
通讯机构：
[Jing'ai Shao; Xu Chen] S;State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, Wuhan 430074, China<&wdkj&>Department of New Energy Science and Technology, School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan 430074, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
作者机构：
[Chen, Qiangwen; Yu, Li; Zhang, Weiwei; Liao, Yongling; Chao, Wei; Ye, Jiabao; Xu, Feng; Liao, Xiaoli] Yangtze Univ, Coll Hort & Gardening, Jinzhou 434025, Peoples R China.;[Chen, Qiangwen; Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.;[Yang, Xiaoyan] Shaoguan Univ, Henry Fok Sch Biol & Agr, Shaoguan 512005, Peoples R China.;[Cheng, Shuiyuan; Cong, Xin] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Qian, Hua; Zhao, Yang] Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China.
通讯机构：
[Feng Xu; Shuiyuan Cheng] A;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Horticulture and Gardening, Yangtze University, Jinzhou 434025, China
摘要：
Selenium (Se) is an important trace element in human and animal health. Approximately 0.5-1 billion people worldwide are facing Se deficiency which can result in various endemic diseases. Cabbage is one of the most popular vegetables and can accumulate Se through biofortification. Therefore, producing Se-enriched cabbage may be an effective method to alleviate Se deficiency. In this study, the effects of different concentrations of selenite application on the growth and nutritional quality of cabbage were investigated and the results showed that the growth of cabbage was promoted at low concentrations of selenite (0.1-0.4 mmol/L) but inhibited at high concentrations (0.8-1.6 mmol/L). Concentrations of 0.1-0.2 mmol/L of selenite induced the accumulation of primary metabolites (soluble proteins, soluble sugars, and free amino acids), representative secondary metabolites (ascorbic acid, glucosinolates, flavonoids, anthocyanins, and phenolic acids), and important antioxidant enzyme (glutathione peroxidase, catalase, and superoxide dismutase) activity to improve the nutritional quality of cabbages. In addition, a higher concentration (0.8-1.6 mmol/L) of selenite proved beneficial in the accumulation of total Se and representative organic Se in cabbages. The main organic Se species in cabbage were selenomethionine (SeMet), accounting for 12.10% of total Se, followed by selenocysteine (SeCys(2)), accounting for 2.96% of total Se. It is suggested that an appropriate dose of exogenous selenite could be selected for different production purposes in cabbage cultivation. These findings are helpful for us to deeply understand the effects of selenite on the growth and nutritional quality of cabbages and to provide reliable technical support for vegetable cultivation and Se biofortification.
作者机构：
[He, Jiangling; Cheng, Shuiyuan; Hu, Yili; Zhou, Jiaojiao; Cai, Jie; Liu, Yuantao] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Chen, Lei; Zhang, Die; Xu, Wei] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Jie Cai] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Liparis Richard (Malaxideae, Epidendroideae) is a large and diverse genus of the family Orchidaceae, the taxonomy of which is complicated and controversial. In this study, we sequenced, assembled and analyzed four complete chloroplast genomes of Liparis species including L. kumokiri, L. makinoana, L. pauliana, and L. viridiflora, and evaluated their phylogenetic relationships with related species for the first time. These four chloroplast genomes (size range 153,095 to 158,239 bp) possess typical quadripartite structures that consist of a large single copy (LSC, 83,533-86,752 bp), a small single copy (SSC, 17,938-18,156 bp) and a pair of inverted repeats (IRs, 26,421-26,933 bp). The genomes contain 133 genes, including 87 protein coding genes, 38 tRNAs and 8 rRNA genes. The genome arrangements, gene contents, gene order, long repeats and simple sequence repeats were similar with small differences observed among these four chloroplast genomes. Five highly variable regions including ycf1, ndhA, ndhF, trnQ and trnK were identified from the comparative analysis with other nine related Liparis species, which had the potential to be used as DNA markers for species identification and phylogenetic studies of Liparis species. Phylogenetic analysis based on the complete chloroplast genome sequences strongly supported the polyphyly of Liparis and its further division into three branches. These results provided valuable information to illustrate the complicated taxonomy, phylogeny and evolution process of the Liparis genus.
作者机构：
[Cheng, Shuiyuan; Rao, Shen; Qiu, Tianci; Deng, Jie; Liu, Haodong; Cong, Xin; Xiao, Chunmei; Cheng, Hua] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Zhang, Yue; Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.
通讯机构：
[Yue Zhang; Shen Rao] A;Authors to whom correspondence should be addressed.<&wdkj&>Enshi Se-Run Material Engineering Technology Co., Ltd., Enshi 445000, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>School of Modern Industry for Selenium Science and Engineering, National R&D Center for Se-Rich Agricultural Products Processing Technology, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Plant growth is affected by various abiotic stresses, including water, temperature, light, salt, and heavy metals. Selenium (Se) is not an essential nutrient for plants but plays important roles in alleviating the abiotic stresses suffered by plants. This article summarizes the Se uptake and metabolic processes in plants and the functions of Se in response to water, temperature, light, salt, and heavy metal stresses in plants. Se promotes the uptake of beneficial substances, maintains the stability of plasma membranes, and enhances the activity of various antioxidant enzymes, thus alleviating adverse effects in plants under abiotic stresses. Future research directions on the relationship between Se and abiotic stresses in plants are proposed. This article will further deepen our understanding of the relationship between Se and plants.
摘要：
Bound phenolic compounds in the melon pulp have seldom been investigated. This study revealed considerable differences in the total phenolic content (TPC) and antioxidant activity of the free and bound phenolic extracts in the pulps of six melon varieties from Hainan Province, China. Naixiangmi and Yugu demonstrated the highest free TPC, while Meilong showed the highest bound and total TPC and antioxidant activity. UHPLC-QQQ-MS identified and quantified 30 phenolic compounds. The melon cultivars markedly differed in the amount and content of their free and bound phenolic compounds. Xizhoumi No. 25 and Meilong afforded the most phenolic compounds. Hongguan emerged with the highest free phenolic compound content and total content of phenolic compounds; however, Meilong possessed the highest bound phenolic compound content. Hierarchical cluster analysis divided the melon varieties into four different taxa. The present study provides a scientific basis for developing the health-promoting effects of melon pulp.
通讯机构：
[Wujun Ma] C;College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China<&wdkj&>Food Futures Institute, College of Science, Health, Engineering and Education, Murdoch University, Perth 6150, Australia<&wdkj&>Author to whom correspondence should be addressed.
关键词：
Chinese steamed bread;wheat gluten proteins;glutenins;gliadins;rheological properties;frozen storage
摘要：
Chinese steamed bread (CSB) is a main staple food in China, accounting for 40% of wheat flour usage in China. Due to its health benefits, CSB is gaining popularity across the world. In this review, the effects of gluten proteins (particularly glutenins and gliadins) on the quality of CSB are summarized from the literature. Requirements of appropriate rheological parameters in different studies are compared and discussed. Along with the increasing demand for frozen storage food, there are obvious increases in the research on the dynamics of gluten proteins in frozen dough. This review also summarizes the factors influencing the deterioration of CSB dough quality during frozen storage as well as effective measures to mitigate the negative effects.
作者机构：
[Li, Xiaoqian; Wang, Zi; Du, Xiubo; Zhang, Huajie; Han, Shuangxue; He, Zhijun; Li, Nan; Liu, Qiong; Tu, Sixin; Shen, Liming] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Biotechnol & Ecol, Shenzhen 518055, Guangdong, Peoples R China.;[He, Zhijun] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Li, Nan; Liu, Qiong] Shenzhen Bay Lab, Shenzhen 518055, Peoples R China.;[Du, Xiubo] Shenzhen Hong Kong Inst Brain Sci, Shenzhen Fundamental Res Inst, Shenzhen 518055, Peoples R China.
通讯机构：
[Qiong Liu] S;Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong 518055, China<&wdkj&>Shenzhen Bay Laboratory, Shenzhen 518055, China
关键词：
Alzheimer's disease (AD);Esculentoside A (EsA);Isobaric tags for relative and absolute;quantitation (iTRAQ);AMP-activated protein kinase (AMPK);Microtubule-associated protein tau (Tau)
摘要：
Background: Neurofibrillary tangles comprising hyperphosphorylated tau are vital factors associated with the pathogenesis of Alzheimer's disease (AD). The elimination or reduction of hyperphosphorylated and abnormally aggregated tau is a valuable measure in AD therapy. Esculentoside A (EsA), isolated from Phytolacca esculenta, exhibits pharmacotherapeutic efficacy in mice with amyloid beta-induced AD. However, whether EsA affects tau pathology and its specific mechanism of action in AD mice remains unclear. Purpose: To investigate the roles and mechanisms of EsA in cognitive decline and tau pathology in a triple transgenic AD (3 x Tg-AD) mouse model. Methods: EsA (5 and 10 mg/kg) was administered via intraperitoneal injection to 8-month-old AD mice for eight consecutive weeks. Y-maze and novel object recognition tasks were used to evaluate the cognitive abilities of mice. Potential signaling pathways and targets in EsA-treated AD mice were assessed using quantitative proteomic analysis. The NFT levels and hippocampal synapse numbers were investigated using Gallyas-Braak silver staining and transmission electron microscopy, respectively. Western blotting and immunofluorescence assays were used to measure the expression of tau-associated proteins. Results: EsA administration attenuated memory and recognition deficits and synaptic damage in AD mice. Isobaric tags for relative and absolute quantitation proteomic analysis of the mouse hippocampus revealed that EsA modulated the expression of some critical proteins, including brain-specific angiogenesis inhibitor 3, galectin-1, and Ras-related protein 24, whose biological roles are relevant to synaptic function and autophagy. Further research revealed that EsA upregulated AKT/GSK3 beta activity, in turn, inhibited tau hyperphosphorylation and promoted autophagy to clear abnormally phosphorylated tau. In hippocampus-derived primary neurons, inhibiting AMP-activated protein kinase (AMPK) activity through dorsomorphin could eliminate the effect of EsA, as revealed by increased tau hyperphosphorylation, downregulated activity AKT/GSK3 beta, and blocked autophagy. Conclusions: To our knowledge, this study is the first to demonstrate that EsA attenuates cognitive decline by targeting the pathways of both tau hyperphosphorylation and autophagic clearance in an AMPK-dependent manner and it shows a high reference value in AD pharmacotherapy research.
作者：
Ye, Yuanyuan;Yan, Wei;Peng, Lijun;He, Jiangling;Zhang, Na;...
期刊：
LWT,2023年175:114470 ISSN：0023-6438
通讯作者：
Jiangling He<&wdkj&>Jie Cai
作者机构：
[He, Jiangling; Cheng, Shuiyuan; Zhou, Jiaojiao; Cai, Jie; Zhang, Na; Ye, Yuanyuan] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Yan, Wei; Peng, Lijun] Hubei Key Lab Nutr Qual & Safety Agroprod, Wuhan 430064, Peoples R China.;[Cai, Jie; Ye, Yuanyuan] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Jiangling He; Jie Cai] N;National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, China
关键词：
Se-enriched green tea;Mineral;Amino acid;Catechins;Correlation analysis
摘要：
Substance basis of selenium (Se) enriched green tea (Se-GT) plays a crucial role in imparting numerous health benefits to the consumer, however, the complex relationship between minerals, chemical ingredients, and Se in tea leaves remains largely unknown. Here, the contents of 14 minerals and 29 bioactive components (including 19 amino acids, 8 polyphenols, and 2 alkaloids) were determined in five green tea samples with different Se levels. Marked differences were observed in the elemental and chemical profiles of the five groups of teas. Pearson correlation analysis demonstrated that Se was likely to be synergistic with Zn, while antagonistic with Mg, Al, Cr, Fe, and As. Inconspicuous correlations between amino acids and Se in tea leaves were discussed via this extended study. Moreover, Se showed positive correlations with gallic acid, (+)-catechin, and gallated catechins (like (-)-epigallocatechin gallate, (-)-gallocatechin gallate, and (-)-epicatechin gallate), but nega-tively correlated with some non-gallated catechins (including (-)-gallocatechin and (-)-epigallocatechin) in tea leaves. These results suggested that Se has significant effects on the accumulation of minerals and polyphenols in tea leaves. Overall, this study elucidates the elemental and chemical basis of Se-GT and provides a rationale for the quality evaluation of Se-GT, which is of crucial importance for tea industry.
作者机构：
Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China;Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, China;Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, China;[Shuiyuan Cheng; Na Zhang; Jiangling He; Jiaojiao Zhou] National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Zhijun He] Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Jie Cai] K;[Jiangling He] N;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
The authors wish to make the following corrections to their published paper [...]
摘要：
Triterpenoids, known for their anti-inflammatory, anticancer, and hypoglycemic properties, are the major bioactive components in Cyclocarya paliurus (Batal.) Iljinskaja. Selecting elite individuals with high triterpenoids content is the basis of C. paliurus industry for medicinal use. In this study, seasonal variation patterns of total triterpenoids and five triterpene monomers accumulation for three groups with different total triterpenoid contents (TTC; H: 59.74-64.03mgg(-1); M: 47.66-57.08mgg(-1), and L: 35.26-42.22mgg(-1)) were surveyed. Seasonal expression dynamics of 6 key genes relevant to triterpenoids biosynthesis, including HMGR, DXR, SQS, SE, LUS, and β-AS, were described by quantitative real-time PCR (qRT-PCR) for three groups. The expression levels of HMGR, SE, LUS, and β-AS genes in group H were higher than in groups M and L. In addition, Pearson correlation analysis showed that they were significantly positively correlated with triterpene accumulation, and the expression level of SE gene not only was significantly correlated with downstream genes, but also exhibited a linear relationship with TTC, especially in September. These results suggest that SE gene could serve as an effective make for screening elite individuals with high TTC from the germplasm of C. paliurus for medicinal use. Further testing on randomly selected individuals in next September proved the feasibility and reliability of SE gene in assisted selection. Also, we successfully cloned the full-length cDNA of SE. Thus, our work provides an efficient way to attain superior genotypes to develop medicinal industry of C. paliurus in practice.
期刊：
Food Research International,2022年161:111811 ISSN：0963-9969
通讯作者：
He, Jingren(jingren.he@whpu.edu.cn)
作者机构：
[Jiang, Tian; He, Jingren] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;[Ye, Shuxin] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Liao, Wei] Univ Lyon, Univ Claude Bernard Lyon 1, CNRS, LAGEPP UMR 5007, 43 Blvd 11 Novembre 1918, F-69100 Villeurbanne, France.;[Wu, Muci; He, Jingren] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.;[Oliveira, Helder; Mateus, Nuno] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE,LAQV, Rua Campo Alegre S-N, P-4169007 Porto, Portugal.
通讯机构：
[Jingren He] S;[Hélder Oliveira] L;LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal<&wdkj&>School of Food Science and Engineering, Wuhan Polytechnic University, 430023 Wuhan, China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
期刊：
International Journal of Biological Macromolecules,2022年213:718-727 ISSN：0141-8130
通讯作者：
Jie Cai
作者机构：
[Zhou, Rui; Wei, Ling-Feng; Cai, Jie; Chen, Lei; Zhang, Die; Yan, Xiao-Qin; Zhu, Wei-Jia; Ding, Wen-Ping] Wuhan Polytech Univ, Minist Educ, Hubei Key Lab Proc & Transformat Agr Prod, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.;[Ma, Tie-Zheng] Beijing Technol & Business Univ, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China.;[Wei, Ling-Feng; Cai, Jie; Zhang, Die; Zhu, Wei-Jia] Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Serich Agr Pro, Wuhan 430023, Peoples R China.;[Din, Zia-ud] Univ Swabi, Dept Agr, Anbar 23561, Khyber Pakhtunk, Pakistan.
通讯机构：
[Jie Cai] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China
关键词：
Anthocyanin;Microgel;Starch
摘要：
We report a self-assembly method for the fabrication of multilayer-starch-based microgels used for anthocyanin encapsulation. Alcohol-heating treatment and ionization reactions were employed to reduce the crystallinity of starch and introduce ionic groups on the molecule to further cross-link it with sodium trimetaphosphate and produce a starch microgel. The molecular interactions between the starch and the anthocyanins facilitated the anthocyanin encapsulation and the starch-microgel/anthocyanin complexes with one, two, and three self-assembled layers were obtained. The Lay-1 microgel exhibited an encapsulation efficiency of 50.1% when the anthocyanin concentration, cross-linking starch concentration, contact time, and temperature were 0.25mg/mL, 1.5mg/mL, 3h, and 40°C, respectively. An increase in the number of layers resulted in a more compact microgel structure with the zeta potential presenting variations upon structural changes. Furthermore, the encapsulated anthocyanins presented a slow release from Lay-1, while the multilayered microgels (Lay-2 and Lay-3) displayed outstanding encapsulation stability. This study gives an insight on the encapsulation and release of anthocyanins by starch microgels, and provides a novel strategy for the design of starch-based encapsulation materials presenting great stability.
