摘要：
Alzheimer's disease (AD) is a devastating neurodegenerative disorder characterized by the deposition of beta-amyloid (A beta) peptides and dysfunction of mitochondrion, which result in neuronal apoptosis and ultimately cognitive impairment. Inhibiting A beta generation and repairing mitochondrial damage are prominent strategies in AD therapeutic treatment. Luteolin, a flavonoid compound, exhibits anti-inflammatory neuroprotective properties in AD mice. However, it is still unclear whether luteolin has any effect on A beta pathology and mitochondrial dysfunction. In this study, the beneficial effect and underlying mechanism of luteolin were investigated in triple transgenic AD (3 x Tg-AD) mice and primary neurons. Our study showed that luteolin supplement significantly ameliorated memory and cognitive impairment of AD mice and exerted neuroprotection by inhibiting A beta generation, repairing mitochondrial damage and reducing neuronal apoptosis. Further research revealed that luteolin could directly bind with peroxisome proliferator-activated receptor gama (PPAR gamma) to promote its expression and function. In the culture of hippocampus-derived primary neurons, addition of PPAR gamma antagonist GW9662 or knockdown of PPAR gamma with its siRNA could eliminate the effect of luteolin on AD pathologies. In summary, this work revealed for the first time that luteolin effectively improved cognitive deficits of 3 x Tg-AD mice and inhibited A beta-induced oxidative stress, mitochondrial dysfunction and neuronal apoptosis via PPAR.dependent mechanism. Hence, luteolin has the potential to serve as a therapeutic agent against AD.
作者机构：
[Wang, Li; Li, Shuyi; Zhu, Zhenzhou; Wang, Xuehua; Gao, Weilan; Zhang, Na; Cong, Xin; Zheng, Zhan] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;[Gao, Weilan] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Liu, Haiyuan; Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.
通讯机构：
[Wang, XH ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.
摘要：
Selenium polysaccharides have attracted significant interest due to their superior function to that of individual polysaccharides. However, limited research has compared the protective effects of different selenium polysaccharides from different selenization methods on diabetes. This work aims to compare the preventive effects of natural selenium-enriched green tea polysaccharides (NSe-TPS), synthetic selenized green tea polysaccharides (PCSe-TPS), and a mixture of sodium selenite and green tea polysaccharides (ordinary tea polysaccharides (Ord-TPS)+Se) on the development of diabetes. While establishing a diabetes model induced by a high-sugar, high-fat diet combined with streptozotocin, different selenium polysaccharides were administered daily by gavage for nine weeks. Our findings indicate that PCSe-TPS exhibited superior preventive effects on developing type 2 diabetes compared to NSe-TPS and Ord-TPS+Se. PCSe-TPS effectively regulated glucose metabolism and insulin resistance by activating the PI3K/Akt pathway, thereby preventing elevated blood glucose levels. Additionally, PCSe-TPS mitigated oxidative damage and inflammatory responses in liver tissues. Notably, PCSe-TPS intervention reversed the decline in bacterial species richness and the abundance of unclassified_Oscillospiraceae during the development of diabetes in mice. These results provide valuable insights into the protective effects of PCSe-TPS against diabetes development, highlighting its advantages over NSe-TPS and Ord-TPS+Se.
摘要：
To evaluate and compare the safety of four selenium supplements, namely Se-enriched peptides (SeP), yeast selenium (SeY), L-Se-methylselenocysteine (L-SeMc) and sodium selenite (Na(2)SeO(3)), the subchronic toxicity study was designed by 90-day gavage administration in Sprague-Dawley rats. The doses of SeP, SeY, L-SeMc and Na(2)SeO(3) were 0.15, 0.30 and 0.60mg/kg bw/day, with additional dose of 0.45mg/kg L-SeMc (All dose calculated as Se). Symptoms like growling, hair loss and significant weight loss were found at 0.60mg/kg of L-SeMc, but not in other groups. At the dose of 0.60mg/kg, females in Na(2)SeO(3), SeY and L-SeMc groups showed significant elevations in ALT and/or ALP. Pathologic manifestations such as bile duct hyperplasia and cholestasis were predominantly found in females at 0.6mg/kg of L-SeMc and SeY groups, and in males at same dose of L-SeMc group showed marked testicular atrophy. 0.60mg/kg of SeY and Na(2)SeO(3), and 0.30, 0.45, 0.60mg/kg of L-SeMc induced significant reductions in sperm motility rates, rapid movement and amount. In conclusion, the NOAEL of SeP, SeY, L-SeMc, Na(2)SeO(3) was all 0.30mg/kg for female, and 0.60, 0.30, 0.15 and 0.30mg/kg for male respectively. Liver and reproductive organs are possible toxic target organs of hyper selenium.
关键词：
Extruded rice bran;Brown rice noodle;Microstructure of brown rice noodle;Moisture migration;Cooking stability
摘要：
Brown rice flour (BRF) and corresponding brown rice noodle (BRN) were prepared by different methods and the mechanism of impact of extruded rice bran on cooking stability of BRN was investigated. The particle size distribution, damaged starch content and pasting properties of BRFs were evaluated and the texture properties, microstructure and moisture distribution during cooking of BRNs were investigated. The average particle size and damaged starch of brown rice flour refilled with extruded rice bran (BRF-E) and brown rice flour refilled with nonextruded rice bran (BRF-NE) were obviously less than that of the brown rice flour by fully crushed brown rice (BRF-F). The peak viscosity (PV), setback viscosity (SV) and pasting temperature (PT) of BRF-E and BRF-NE were significantly higher than that of BRF-F. The results of scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) showed that the microstructure of BRN-E was most compact, which indicated that using BRF refilled with extruded rice bran to produce BRN could promote BRN formed a compact texture structure. Meanwhile, water migration and texture properties analysis demonstrated that BRN-E had better cooking stability and chewiness.
作者机构：
[He, Yi; Wang, Zhangqian; Gao, Chao; Deng, Jieya; Chen, Jixin] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Wuhan 430023, Peoples R China.;[Mohamed, Hany I.] Benha Univ, Fac Sci, Chem Dept, Banha 13518, Egypt.;[Umer, Muhammad] Guizhou Univ, Inst Forest Resources & Environm Guizhou, Guiyang 550025, Peoples R China.;[Umer, Muhammad] Guizhou Univ, Forestry Coll, Res Ctr Forest Ecol, Guiyang 550025, Peoples R China.;[Anwar, Naureen] Univ Narowal, Dept Zool, Narowal 51600, Punjab, Pakistan.
通讯机构：
[Chao Gao; Yi He] N;National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
G-quadruplexes (G4s) are widely found in cells and have significant biological functions, which makes them a target for screening antitumor and antiviral drugs. Most of the previous research on G4s has been conducted mainly in diluted solutions. However, cells are filled with organelles and many biomolecules, resulting in a constant state of a crowded molecular environment. The conformation and stability of some G4s were found to change significantly in the molecularly crowded environment, and interactions with ligands were disturbed to some extent. The structure of the G4s and their biological functions are correlated, and the effect of the molecularly crowded environment on G4 conformational transitions and interactions with ligands should be considered in drug design targeting G4s. This review discusses the changes in the conformation and stability of G4s in a physiological environment. Moreover, the mechanism of action of the molecularly crowded environment affecting the G4 has been further reviewed based on previous studies. Furthermore, current challenges and future research directions are put forward. This review has implications for the design of drugs targeting G4s.
摘要：
Selenium (Se) biofortification of crops has been studied to substantially improve the Se content in human dietary food intake. In the present study, Vigna radiata (mung bean) seeds were soaked in different concentrations of sodium selenite (Na(2)SeO(3)). Low concentration of selenite is conducive to seed germination and growth, and can increase the fresh weight (FW) and dry weight (DW) of sprouts. The concentration of Na(2)SeO(3) lower than 50mg/kg resulted in noticeable elongation in the stem and marginal elongation in root. Mung bean seeds soaked with 80mg/kg Na(2)SeO(3) accounted for 93.77% of organic Se after growing for about 5days. Transcriptome data revealed that Se treatment enhances starch and sugar metabolism, along with the up-regulation of ribosomal protein and DNA synthesis related genes. Further analysis indicated that the mung bean seeds absorbed Na(2)SeO(3) through PHT1.1 and NIP2. Se (IV) was transformed into Se (VI) and transported to stems, leaves and roots through cotyledons during the germination of bean sprouts. SULTR3;3 may play an important role in the transit process. Se (VI) or Se (IV) transported to the leaves was catalytically transformed into SeCys through SiR and CS, and SeCys is further converted to MeSeCys through SMT. Most SeCys were transformed into SeHCys through CBL, transported to plastids, and finally transformed into SeMet through Met Synthase.
期刊：
Results in Engineering,2023年20:101530 ISSN：2590-1230
通讯作者：
Benkun Qi
作者机构：
[Zhenzhou Zhu] Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;National R&D Center for Se-rich Agricultural Products Processing, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[Benkun Qi] State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China;[Caixia Wang] Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China;University of Chinese Academy of Sciences, Beijing, 100049, China
通讯机构：
[Benkun Qi] S;State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China
摘要：
The utilization of herbal extraction residues (HERs) for production of valuable products has gained significant momentum in recent years. In this study, Lianhua Qingwen residue (LQR) was selected as a representative HER for enzymatic saccharification. By subjecting starch-containing raw unpretreated LQR to direct hydrolysis using a combination of cellulolytic and amylolytic enzyme, a glucan conversion of around 60 % was achieved within 48 h. To further enhance the hydrolysis rate and yield, ultrasound treatment of LQR samples was conducted. Investigation into the effect of ultrasound treatment on subsequent enzymatic saccharification revealed a noteworthy acceleration in the saccharification rate of LQR, exceeding 20 % within 10 h of hydrolysis time. Notably, this enhancement was achieved even when the substrate concentration was high at 10 % under optimized conditions of 5 min of sonication at a power intensity of 1.20 kW/cm2 and a temperature of 50 °C. The improvement in efficiency can be attributed to the disruptive effect of ultrasound treatment on the structure of LQR, leading to a reduction in particle size. Consequently, the LQR particles exhibited better contact with the enzyme, promoting a more efficient saccharification process. These findings suggest that ultrasound technology holds considerable potential for enhancing the enzymatic saccharification of HERs.
作者机构：
[He, Yi; Wang, Zhangqian; Gao, Chao; He, Y; Deng, Jieya; Chen, Jixin] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Anwar, Naureen] Virtual Univ Pakistan, Fac Sci & Technol, Dept Biol Sci, Lahore 54000, Pakistan.;[Umer, Muhammad] Guizhou Univ, Inst Forest Resources & Environm, Guizhou Forestry Coll, Res Ctr Forest Ecol, Guiyang 550025, Peoples R China.
通讯机构：
[Umer, M ] G;[He, Y ] W;Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;Guizhou Univ, Inst Forest Resources & Environm, Guizhou Forestry Coll, Res Ctr Forest Ecol, Guiyang 550025, Peoples R China.
摘要：
Human telomere sequences (TTAGGG)(n) fold into G-quadruplexes with different conformations in K(+) and Na(+) solutions, which are highlighted for their potential as antitumor drug targets. Moreover, human multimeric G-quadruplexes have been broadly studied potentially for screening ligands with higher selectivity than monomeric G-quadruplexes. Most insects have telomeres consisting of pentanucleotide (TTAGG) repeats, which fold into an antiparallel structured G-quadruplex with a two-layer G-planar in a K(+) solution. However, the structure of insect telomeric G-quadruplexes in Na(+) solutions and their higher-order structures have not been explored. The quinoline derivative BMPQ-1 has been reported to bind human multimeric G-quadruplex. This study compared the stability and compactness of insect monomeric and multimeric G-quadruplex structures in K(+) and Na(+) solutions and further validated the interaction between BMPQ-1 and insect multimeric G-quadruplexes. Circular dichroism (CD) spectral scanning analysis revealed that although the insect telomeric G-quadruplex folds into an antiparallel structure in both K(+) and Na(+) solutions, all the insect telomeric G-quadruplexes are more stable in Na(+) solutions. Fluorescence resonance energy transfer (FRET) analysis indicated insect telomeric G-quadruplexes have a more compact structure in Na(+) solutions. BMPQ-1 exhibited higher selectivity for insect multimeric G-quadruplex Bom37 than monomeric G-quadruplex Bom17, and had a different binding pattern to Bom37 G-quadruplex in K(+) and Na(+) solutions. Finally, BMPQ-1 was found to have a significant inhibitory effect on the proliferation of pest cells. This study contributes to our comprehensive understanding of insect telomeric G-quadruplexes.
作者：
Ye, Yuanyuan;Yan, Wei;Peng, Lijun;Zhou, Jiaojiao;He, Jiangling;...
期刊：
Food Research International,2023年165:112460 ISSN：0963-9969
通讯作者：
Jiangling He<&wdkj&>Na Zhang<&wdkj&>Jie Cai
作者机构：
[He, Jiangling; Cheng, Shuiyuan; Zhou, Jiaojiao; Cai, Jie; Zhang, Na; Ye, Yuanyuan] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Yan, Wei; Peng, Lijun] Hubei Key Lab Nutr Qual & Safety Agroprod, Wuhan 430064, Peoples R China.;[Cai, Jie; Ye, Yuanyuan] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Jiangling He; Na Zhang; Jie Cai] N;National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
关键词：
Amino acid;Correlation analysis;Key aroma compound;Polyphenol;Se-enriched green tea;Selenium
摘要：
Selenium (Se) deficiency causes impaired growth of fish skeletal muscle due to the retarded hypertrophy of muscle fibers. However, the inner mechanisms remain unclear. According to our previous researches, we infer this phenomenon is associated with Se deficiency-induced high concentration of reactive oxygen species (ROS), which could suppress the target of rapamycin complex 1 (TORC1) pathway-mediated protein synthesis by inhibiting protein kinase B (Akt), an upstream protein of TORC1. To test this hypothesis, juvenile zebrafish (45 days post fertilization) were fed a basal Se-adequate diet or a basal Se-deficient diet, or them supplemented with an antioxidant (DL-α-tocopherol acetate, designed as VE) or a TOR activator (MHY1485) for 30 days. Zebrafish fed Se-deficient diets exhibited a clear Se-deficient status in skeletal muscle, which was not influenced by dietary VE and MHY1485. Se deficiency significantly elevated ROS concentrations, inhibited Akt activity and TORC1 pathway, suppressed protein synthesis in skeletal muscle, and impaired hypertrophy of skeletal muscle fibers. However, these negative effects of Se deficiency were partly (except that on ROS concentration) alleviated by dietary MHY1485 and completely alleviated by dietary VE. These data strongly support our speculation that Se deficiency-induced high concentration of ROS exerts a clear inhibiting effect on TORC1 pathway-mediated protein synthesis by regulating Akt activity, thereby restricting the hypertrophy of skeletal muscle fibers in fish. Our findings provide a mechanistic explanation for Se deficiency-caused retardation of fish skeletal muscle growth, contributing to a better understanding of the nutritional necessity and regulatory mechanisms of Se in fish muscle physiology.
期刊：
Environmental Science and Pollution Research,2023年30(36):86232-86243 ISSN：0944-1344
通讯作者：
Qi, BK
作者机构：
[Zhu, Zhenzhou] Wuhan Polytech Univ, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Wu, Sirong] Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.;[Wu, Sirong; Qi, Benkun; Luo, Jianquan] Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China.;[Wang, Caixia] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China.;[Wan, Yinhua; Luo, Jianquan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
通讯机构：
[Qi, BK ] C;Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China.
