作者机构：
[Cheng, Shuiyuan; He, Yi; Zhu, Lisha; Dong, Xingxing; Xie, Chaoxin; He, Y; He, Jingren] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.;[He, Yi; Zhu, Lisha; Xie, Chaoxin; Su, Yue] Wuhan Polytech Univ, Sch Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Key Lab Deep Proc Major Grain & Oil,Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[He, Y ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.
关键词：
Selenium-enriched black sesame pigment;Process optimization;Structural characterisation;Fourier infrared spectroscopy;Antioxidant activities
摘要：
In recent decades, the development and utilization of selenium (Se) resources have attracted considerable attention based on evidence that Se deficiency is associated with some human diseases. In this study, Enshi Se-enriched Sesamum indicum L. [black sesame (BS)] pigment (BSP) was extracted by ultrasonic-assisted extraction with process optimization, the highest BSP yield of 1.36% was achieved when ultrasound temperature, extraction pH and ultrasound power was 73 degrees C, 2.9 and 273 W, respectively. After purification and lyophilization, the total Se content of Se-enriched BSP (2.427 +/- 0.102 mu g/g) was found to be 5.48-fold that of control BSP (0.443 +/- 0.012 mu g/g). Full-wavelength ultraviolet scanning and Fourier infrared (FI-IR) spectroscopy revealed that the combination of Se and BSP contributed to the typical absorption peak red-shift and new peak of Se-enriched BSP, respectively. Furthermore, the antioxidant activities of Se-enriched BSP against hydroxyl radical, DPPH radical, and ABTS radical at 2.5 mg/mL were 78.6%, 82.1%, and 2.94 mmol/Trolox, respectively, indicating that Se-enriched BSP possesses greater antioxidant capacity than control BSP. Based on the significant Se content and good antioxidant characteristics, Se-enriched BSP can be considered as a functional pigment for potential application in the food and pharmaceutical industry.
通讯机构：
[Shuiyuan Cheng; Hua Cheng] A;Authors to whom correspondence should be addressed.<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Cadmium (Cd) is a common agricultural soil pollutant, which does serious harm to the environment and the human body. In this study, Brassica juncea was treated with different concentrations of CdCl2 and Na2SeO3. Then, physiological indexes and transcriptome were measured to reveal the mechanisms by which Se reduces the inhibition and toxicity of Cd in B. juncea. The results showed that Se alleviated the inhibitive Cd effects on seedling biomass, root length, and chlorophyll, and promoted the adsorption of Cd by pectin and lignin in the root cell wall (CW). Se also alleviated the oxidative stress induced by Cd, and reduced the content of MDA in cells. As a result, SeCys and SeMet alleviated the transport of Cd to the shoots. Transcriptome data showed that the bivalent cation transporter MPP and ABCC subfamily participated in the separation of Cd in vacuoles, CAL1 was related to the chelation of Cd in the cytoplasm of cells, and ZIP transporter 4 reduced the transport of Cd to the shoots. These results indicated that Se alleviated the damage of Cd in plants and decreased its transport to the shoots by improving the antioxidant system, enhancing the ability of the CW to adsorb Cd, reducing the activity of Cd transporters, and chelating Cd.
期刊：
International Journal of Biological Macromolecules,2023年240:124442 ISSN：0141-8130
通讯作者：
He, Yi;Wang, ZQ
作者机构：
[He, Yi; Dong, Xingxing; Wang, Zhangqian; Wang, ZQ; Gao, Chao; Xu, Hua; Deng, Jieya; He, Y; Chen, Jixin] Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Wang, Zhangqian; Wang, ZQ] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China.;[Anwar, Naureen] Univ Narowal, Dept Zool, Narowal 51600, Punjab, Pakistan.;[Umer, Muhammad] Guizhou Univ, Inst Forest Resources & Environm Guizhou, Guiyang 550025, Peoples R China.;[Umer, Muhammad] Guizhou Univ, Forestry Coll, Res Ctr Forest Ecol, Guiyang 550025, Peoples R China.
通讯机构：
[Wang, ZQ ; He, Y] W;Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China.
关键词：
A looser structure;Aggregation;Molecular crowding;Parallel structured G-quadruplexes
摘要：
G-quadruplexes are widely distributed in cells and are usually essential in mediating biological processes. The intracellular environment is often in a state of molecular crowding, and the current research considerably focuses on the effect of molecular crowding on the conformation of telomeric G-quadruplexes. However, G-quadruplex-forming oligonucleotides are primarily located in the promoter region of the proto-oncogene and on mRNA inside the cell and are reported to fold into parallel structures. Thus, studying the interaction mechanism between ligands and parallel structured G-quadruplexes under crowding conditions is crucial for the design of drugs targeting G-quadruplexes. In our study, molecular crowding was simulated through polyethylene glycol with an average molecular weight of 200 (PEG200) to investigate the parallel structure of the canonical G-quadruplexes c-KIT1, c-MYC, and 32KRAS and their interactions with ligands. Circular dichroism (CD) spectral scanning, fluorescence resonance energy transfer (FRET), and native polyacrylamide gel electrophoresis (PAGE) analysis revealed that molecular crowding failed to induce oligonucleotides to form parallel G-quadruplex structures in the explored model sequences while induced telomeric G-rich sequences to form antiparallel G-quadruplexes in solution without K(+). Molecular crowding did not induce changes in their parallel structures but promoted the formation of G-quadruplex aggregates. Moreover, to some extent, molecular crowding also induced a looser structure of the monomer G-quadruplexes. Further studies showed that molecular crowding did not alter the binding stoichiometry of the ligand 3,11-difluoro-6,8,13-trimethyl-8H-quino [4,3,2-kl] acridinium methosulfate (RHPS4) to c-KIT1, while it inhibited its interaction with parallel structured G-quadruplexes. This work provides new insights into developing anticancer drugs targeting parallel structured G-quadruplexes.
作者机构：
[He, Jiangling; Zhou, Jiaojiao; Cai, Jie; Qin, Zhiqin; Ye, Yuanyuan] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Qin, Zhiqin; Ye, Yuanyuan; Zhang, Die] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Jiangling He; Jie Cai] N;National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
作者机构：
[He, Jiangling; Cheng, Shuiyuan; Zhou, Jiaojiao; Cai, Jie; Ye, Yuanyuan; Wang, Zhenyu; Cong, Xin; Wei, Lingfeng] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Ye, Yuanyuan; Wang, Zhenyu] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[Din, Zia-ud] Women Univ Swabi, Dept Food Sci & Nutr, Swabi 23430, Khyber Pakhtunk, Pakistan.
通讯机构：
[Jie Cai] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
作者机构：
[Huang, Jian; Li, Shuyi; Zhu, Zhenzhou] Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.;[Huang, Jian; Wang, Lan; Xiong, Guangquan] Hubei Acad Agr Sci, Minist Agr & Rural Affairs, Inst Agr Prod Proc & Nucl Agr Technol, Key Lab Cold Chain Logist Technol Agroprod, Wuhan 430064, Peoples R China.;[Li, Shuyi; Zhu, Zhenzhou] Wuhan Polytech Univ, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Zhang, Yun] Hubei Univ Econ, Coll Tourism & Hotel Management, Wuhan 430205, Peoples R China.
通讯机构：
[Shuyi Li] A;[Guangquan Xiong] K;Key Laboratory of Cold Chain Logistics Technology for Agro-Product, Institute of Agricultural Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430064, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Selenium (Se) is a vital micronutrient for human beings, and the global population facing Se deficiency is estimated to be around one billion individuals. To tackle this issue, the enrichment of staple crops with Se has emerged as a potential solution. However, it is important to note that Se can also be detrimental in excessive amounts, and contamination of the environment due to Se from agricultural and industrial sources has resulted in catastrophic ecological disasters over the past half-century. Consequently, the utilization of Se-enriched plants for both human supplementation and phytoremediation purposes has become an invaluable approach towards pollution control. An in-depth comprehension of how plants absorb and metabolize Se is pivotal in the realms of biofortification and phytoremediation. This comprehensive review concisely outlines the origins, mechanisms of absorption, conversion, and metabolism of Se in plants, while also elucidating the various factors that influence its uptake and accumulation. These influential factors encompass soil moisture, organic matter, pH levels, soil texture, microorganisms, and unique plant species characteristics. Furthermore, a thorough analysis of the potential mechanisms that underlie such influences is conducted. It is evident that both biofortification and phytoremediation possess substantial promise in confronting the challenges pertaining to Se, thereby fostering advancements in environmental sustainability. Building upon the current progress in research, this review provides suggestions for future directions aimed at establishing a theoretical framework for Se supplementation in human nutrition and the mitigation of Se-induced pollution.
摘要：
Plasma-catalytic pyrolysis was developed for upgrading polypropylene (PP) pyrolysis volatiles to co-produce carbon nanotubes (CNTs) and hydrogen. To uncover the role of plasma on the plastic catalytic pyrolysis process, the pyrolysis of polypropylene (PP) over Fe/gamma-Al2O3 was carried out in a two-stage pyrolysis system with a coaxial dielectric barrier discharge (DBD) plasma reactor. The results showed that the plastic pyrolysis volatiles were further cleaved and activated with plasma, resulting in more active carbon species for the growth of CNTs. Compared to conventional catalytic pyrolysis, plasma addition shifted the initial formation temperature of CNTs to a lower ambient temperature by -100 degrees C, and significantly promoted the conversion of liquid and gaseous products to CNTs and hydrogen, with higher carbon and hydrogen yields of -322 mg/gplastic and 30 mmol/ gplastic, respectively. In addition, the degree of graphitization of the CNTs in the presence of the plasma was significantly enhanced with less defectivity. The influence of catalytic temperature variation caused by plasma on CNTs growth was also discussed from the perspective of volatile evolution. This work highlights the potential of plasma-catalytic pyrolysis for the production of hydrogen and high-value carbon materials from plastic waste.
摘要：
The key to advancing urbanization is to promote the urban integration of numerous migrant workers. Two stages of decision making are involved for migrant workers, including residence (staying in cities) and settlement (transferring hukou into cities). The homestead is a necessity for migrant workers to keep their “peasant” status, which will further affect migrant workers’ identification with cities and influence their decision making towards urbanization. This paper uses data from the 2017 China Migrants Dynamic Survey (CMDS), through the coarsened exact matching (CEM) method and the analysis of mediation effects, to estimate how homesteads influence migrant workers’ urbanization intention and how the sense of identity serves as a mediator variable in this mechanism. Empirical results show that the ownership of homesteads is negatively correlated with migrant workers’ urbanization intention. Migrant workers with homesteads are 1.2% less likely to stay and 4.4% less likely to settle down in cities compared with their counterparts who do not have a homestead. In addition, identity plays a mediating role in the influence mechanism of homesteads on migrant workers’ urbanization intention. That is, the homestead has an indirect effect on migrant workers’ willingness to stay and settle down in cites through the sense of identity, aside from its direct effects. The mediation effect accounts for 20.87% of the total effect for willingness to stay and 25.63% of the total effect for willingness to settle down. This paper also represents how these coefficients vary by different regions and migration distances. Therefore, policymakers should provide institutional support for correctly guiding migrant workers to “abandon their land and enter the city” and strengthen their sense of identity to the city.
摘要：
Natural bioactive molecules have been widely used as stabilizers in the functional improvement of selenium nanoparticles (SeNPs) in recent years. In this study, Morchella sextelata polysaccharide (MSP) was introduced as a novel stabilizer for the synthesis of SeNPs based on the redox system of sodium selenite and ascorbic acid. The size, morphology, stability, and anti-cancer cell activities were respectively analyzed by various methods. The results showed that the synthesized SeNPs with MSP were 72.07±0.53nm in size, red in color, spherical in shape, and amorphous in nature. MSP-SeNPs showed high scavenging activity against DPPH and ABTS radicals. And, these MSP-SeNPs exhibited a significant anti-proliferation effect on human liver (HepG2) and cervical cancer (Hela) cells in vitro, while no significant cytotoxicity against normal human kidney cells (HK-2) was observed. Moreover, the mitochondria-dependent apoptosis pathway triggered by MSP-SeNPs in HepG2 cell was identified. The expression levels of p53, Bax, cytochrome c, caspase-3 and caspase-9 were all up-regulated in HepG2 cells after MSP-SeNPs treatment, while Bcl-2 expression was down-regulated. These results suggest that MSP-SeNPs have strong potential as the food supplement for application in cancer chemoprevention.
期刊：
Food Research International,2023年172:113173 ISSN：0963-9969
通讯作者：
Li, Shuyi;Zhu, ZZ
作者机构：
[Zhu, Zhenzhou; Jian, Mengjiao; Li, Shuyi; Zhang, Na; Li, SY] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pr, Wuhan 430023, Peoples R China.;[Jian, Mengjiao] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.;[Deng, Qianchun] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr, Wuhan 430062, Peoples R China.;[Deng, Qianchun] Minist Agr, Key Lab Oilseeds Proc, Wuhan 430062, Peoples R China.;[Cravotto, Giancarlo] Univ Turin, Dept Drug Sci & Technol, I-10125 Turin, Italy.
通讯机构：
[Zhu, ZZ ; Li, SY] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pr, Wuhan 430023, Peoples R China.
关键词：
Covalent interaction;Polyphenol;Polysaccharide;Soy protein isolate;Stability;β-carotene-loaded emulsion
摘要：
A β-carotene rich emulsion with improved physical and chemical stability was obtained in this study, using different types of protein-polysaccharide-polyphenol ternary complexes as novel emulsifiers. The ternary complexes were prepared by covalent or non-covalent binding of soy protein isolate (SPI), β-glucan (DG) and myricetin (MC), which were evidenced to be stable. It was indicated that the emulsion stabilized by covalent complex of SPI, DG and MC, exhibited higher zeta-potential and smaller particle size than those stabilized by non-covalent complex. Furthermore, the covalent complexes prepared from different addition sequences showed different efficiencies in stabilizing the emulsion, in which SPI-DG-MC and SPI-MC-DG-stabilized emulsions possess better stability, emulsifying activity and storage resistance under adverse environmental treatment, with CI values of 62.7% and 64.3% after 25days, respectively. According to oxidative stability and rheology analysis of the emulsions, it was found that the SPI-MC-DG complex prepared at the ratio of 4:2:1 was more stable with relatively less lipid oxidation products and a tighter stacking structure, and the final LH value was 39.98mmol/L and the MDA value was 6.34mmol/L. These findings implied that the ternary complex has the potential to deliver fat-soluble active ingredient by means of emulsion, but which depends on the mode and sequence of the molecular interactions.
作者机构：
[Sun, Ying; Wang, Hongxun; Peng, Kaidi; Zhang, Rui; Yi, Yang; Xu, Wei; Li, Yin] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;[Sun, Ying; Wang, Hongxun; Peng, Kaidi; Yi, Yang; Xu, Wei] Hubei Ind Technol Res Inst Jingchu Special Foods, Jingzhou 434000, Peoples R China.;[Zhang, Rui] Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Peng, Kaidi] 68,Xuefu South Rd,Changqing Garden St, Wuhan 430023, Peoples R China.
通讯机构：
[Yang Yi] H;Hubei Industrial Technology Research Institute of Jingchu Special Foods, Jingzhou 434000, China<&wdkj&>Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
作者：
An, Jiejie;Liu, Mingzhu;Din, Zia-ud;Xie, Fang*;Cai, Jie
期刊：
International Journal of Biological Macromolecules,2023年247:125697 ISSN：0141-8130
通讯作者：
Xie, Fang;Cai, J
作者机构：
[Cai, Jie; An, Jiejie; Liu, Mingzhu; Xie, Fang] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[Din, Zia-ud] Women Univ Swabi, Dept Food Sci & Nutr, Swabi 23430, Khyber Pakhtunk, Pakistan.;[Cai, Jie; Xie, F] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J ; Xie, F] ;Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Delivery;Nanogels;Starch
摘要：
Nanogels formed by self-assembly of natural proteins and polysaccharides have attracted great interest as potential carriers of bioactive molecules. Herein, we reported that carboxymethyl starch-lysozyme nanogels (CMS-Ly NGs) were prepared using carboxymethyl starch and lysozyme by green and facile electrostatic self-assembly, and the nanogels served as epigallocatechin gallate (EGCG) delivery systems. The dimensions and structure of the prepared starch-based nanogels (i.e., CMS-Ly NGs) were characterized by dynamic light scattering (DLS), ζ-potential, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal gravimetric analyzer (TGA). FT-IR and (1)H NMR spectra together confirmed the formation of CMS; FT-IR spectra confirmed the formation of CMS-Ly NGs; XRD spectra confirmed the disruption of the crystal structure of lysozyme after electrostatic self-assembly with CMS, and further confirmed the formation of nanogels. TGA demonstrated the thermal stability of nanogels. More importantly, the nanogels showed a high EGCG encapsulation rate of 80.0±1.4%. The CMS-Ly NGs encapsulated with EGCG exhibited regular spherical structure and stable particle size. Under the simulated gastrointestinal environmental conditions, CMS-Ly NGs encapsulated with EGCG showed the controlled release potential, which increased its utilization. Additionally, anthocyanins can also be encapsulated in CMS-Ly NGs and showed slow-release properties during gastrointestinal digestion in the same way. Cytotoxicity assay also demonstrated good biocompatibility between CMS-Ly NGs and CMS-Ly NGs encapsulated with EGCG. The findings of this research suggested the potential application of protein and polysaccharides-based nanogels in the delivery system of bioactive compounds.
