作者机构：
[Jia, Jilai; Zhou, Jiaojiao; Cai, Jie; Zhou, JJ; Lv, Xuqin; Xie, Fang] Wuhan Polytech Univ, Sch Modern Industryfor SeleniumScience & Engn, NationalR&D CenterforSe richAgriculturalProductsPr, Wuhan 430023, Peoples R China.;[Cai, Jie; Lv, Xuqin] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.;[Du, Xiaoping] Minist Agr & Rural Affairs, Key Lab Se Enriched Prod Dev & Qual Control, Ankang R&D Ctr Se Enriched Prod, Ankang 725000, Shaanxi, Peoples R China.
通讯机构：
[Cai, J ; Zhou, JJ] W;Wuhan Polytech Univ, Sch Modern Industryfor SeleniumScience & Engn, NationalR&D CenterforSe richAgriculturalProductsPr, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.
关键词：
mercury ion detection;colorimetric biosensor;DNA;graphene oxide;platinum nanoparticles
摘要：
Mercury ions (Hg2+) have persistent bioaccumulation and high toxicity, which can severely damage the ecological system and human health. Therefore, sensitive methods are required for Hg2+ analysis. Herein, a DNA-mediated growth strategy was developed for the synthesis of platinum nanoparticles (PtNPs) on the surface of graphene oxide (GO). The resulting GO-DNA-PtNPs displayed excellent peroxidase-mimicking activity in a sequence-dependent manner. The peroxidase-mimicking activity could be significantly inhibited by Hg2+ because of the specific trapping of Hg2+ by thymine bases (T–Hg2+–T) and in situ reduction of Hg2+ by PtNPs on the GO surface. Under optimal conditions, there was a good linear range from 1 pM to 1 μM with a detection limit of 2.2 pM for Hg2+ analysis. This sensor demonstrated excellent analytical performance for assessing Hg2+ in complex lake waters. This work provides an effective strategy for the preparation of PtNP-based nanozymes and broadens the applications of PtNPs for biosensing.
作者机构：
[Li, Xiaoqian; Zheng, Kaixin; Han, Shuangxue; He, Zhijun; Liu, Qiong; Ni, Jiazuan; Huang, Yingying] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Biotechnol & Ecol, Shenzhen 518055, Peoples R China.;[Han, Shuangxue] Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen 518060, Peoples R China.;[Xiao, Peng; Hu, Xia; Xie, Qingguo] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.;[Liu, Qiong; Ni, Jiazuan] Shenzhen Bay Lab, Shenzhen 518055, Peoples R China.;[Liu, Qiong; Ni, Jiazuan] Shenzhen Fundamental Res Inst, Shenzhen Hong Kong Inst Brain Sci, Shenzhen 518055, Peoples R China.
通讯机构：
[Qingguo Xie] C;[Qiong Liu] S;College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China<&wdkj&>Department of Medical Physics and Biomedical Engineering, Istituto Neurologico Mediterraneo, Neuromed IRCCS, 86077 Pozzilli, Italy<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Shenzhen Bay Laboratory, Shenzhen 518055, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen 518055, China<&wdkj&>Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, China
期刊：
Ecotoxicology and Environmental Safety,2023年267:115634 ISSN：0147-6513
通讯作者：
Xiao, J
作者机构：
[Xiao, J; Guo, Zhiqiang; Xiao, Juan; He, Yuke; Peng, Ziting] Hainan Univ, Engn Res Ctr Utilizat Trop Polysaccharide Resource, State Key Lab Marine Resource Utilizat South China, Minist Educ,Key Lab Food Nutr & Funct Food Hainan, Haikou 570228, Peoples R China.;[Wu, Qian] Hubei Univ Technol, Natl Ctr Cellular Regulat & Mol Pharmaceut 111, Hubei Res Ctr Food Fermentat Engn & Technol, Key Lab Fermentat Engn,Minist Educ,Hubei Key Lab I, Wuhan, Peoples R China.;[Li, Shuyi; Zhu, Zhenzhou] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Grimi, Nabil] Sorbonne Univ, Univ Technol Compiegne, Ctr Rech Royallieu, ESCOM,EA 4297,TIMR,CS 60319, F-60203 Compiegne, France.
通讯机构：
[Xiao, J ] H;Hainan Univ, Engn Res Ctr Utilizat Trop Polysaccharide Resource, State Key Lab Marine Resource Utilizat South China, Minist Educ,Key Lab Food Nutr & Funct Food Hainan, Haikou 570228, Peoples R China.
关键词：
Arsenic species;Inorganic arsenic;Seaweeds;Health risk assessment;Monte Carlo simulation
摘要：
Arsenic (As) is a notorious toxic contamination in marine environments, while the toxicity and health risk of As is highly dependent on As species in seafoods. In this study, we hypothesized that the species-specific As bioaccumulation and species resulted in species-specific healthy risk of As in seaweeds. To test the hypothesis, we collected 10 common edible seaweeds from the coast of Hainan Island in South China Sea. Then we comparatively quantified concentration of total As and 5 major As species [AsB, DMA, MMA, As(III), and As(V)] in seaweeds. The results revealed that the concentrations of total As varied significantly among 10 seaweed species. Specially, the highest total As concentration were found in brown seaweeds, followed by red seaweeds, and green seaweeds. Furthermore, the percentage of 5 As species to total As differed significantly among 10 seaweeds. The percentage of AsB was highest in Caulerpa lentillifera (53%) and lowest in Sargassum oligocystum (13%), while that of As(V) was lowest in Caulerpa lentillifera (21%) and highest in Sargassum oligocystum (81%). The iAs [As(III) +As(V)] exhibited highest value in brown seaweeds and least value in green seaweeds. The potential human health risk assessment indicated that the consumption of brown seaweeds of Sargassum oligocystum and Sargassum polycystum could cause a considerable carcinogenic risk and non-carcinogenic risk to residents. Overall, our findings here largely validated our hypothesis that the species-specific As bioaccumulation and As species had great significance to healthy risk of As in seaweeds.
期刊：
Journal of Food Measurement and Characterization,2023年17(2):1703-1713 ISSN：2193-4126
通讯作者：
Zhao Qin<&wdkj&>Xue-De Wang
作者机构：
[Wang, Shou-Tao; Qin, Zhao; Dan, Ya-Qian; Liu, Hua-Min; Lv, Ting-Ting; Ma, Yu-Xiang; Zhang, Chen-Xia; Wang, Xue-De] Henan Univ Technol, Coll Food Sci & Technol, Zhengzhou 450001, Peoples R China.;[Wang, Shou-Tao; Qin, Zhao; Liu, Hua-Min; Lv, Ting-Ting; Ma, Yu-Xiang; Zhang, Chen-Xia; Wang, Xue-De] Henan Univ Technol, Inst Special Oilseed Proc & Technol, Zhengzhou 450001, Peoples R China.;[He, Jing-Ren] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[He, Jing-Ren] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Zhao Qin; Xue-De Wang] C;College of Food Science and Technology, Henan University of Technology, Zhengzhou, China<&wdkj&>Institute of Special Oilseed Processing and Technology, Henan University of Technology, Zhengzhou, China<&wdkj&>College of Food Science and Technology, Henan University of Technology, Zhengzhou, China<&wdkj&>Institute of Special Oilseed Processing and Technology, Henan University of Technology, Zhengzhou, China
摘要：
Subcritical water-ethanol extraction (SWE) was performed to extract proanthocyanidins (PAs) from Chinese quince fruits. The extraction parameters (temperature, time, liquid-solid ratio) were optimized using response surface methodology (RSM), based on single-factor experiments. The optimal SWE conditions were: temperature, 135 degrees C; time, 31 min; liquid solid ratio, 32 mL/g. The maximum PAs obtained were 22.36 +/- 0.35 mg PB2/g DW, which matched predicted values. In the analysis of PAs, 7 compounds were identified: catechin, epicatechin, epicatechin gallate, epigallocatechin-3-gallate, and proanthocyanidins dimers, trimers and tetramers. Structurally, these PAs contained substantial procyanidin (PC) but little prodelphinidin (PD). The findings revealed that SWE yielded more total PA than the conventional method extraction (CME). Furthermore, compared with CME PAs, PAs obtained by SWE showed higher radical scavenging capacities (IC50: 139.32 mu g/mL for DPPH; IC50: 113.11 mu g/mL for ABTS) and stronger alpha-amylase inhibition (IC50: 88.29 mu g/mL). These results indicate that SWE is an efficient method for the separation of PAs from Chinese quince. Chinese quince PAs represent a potentially valuable resource for use in functional foods and nutraceuticals.
作者：
Ye, Yuanyuan;He, Jiangling;He, Zhijun;Zhang, Na;Liu, Xiaoqing;...
期刊：
Foods,2023年12(6):1339- ISSN：2304-8158
通讯作者：
Cai, J.;He, J.
作者机构：
[He, Jiangling; Cheng, Shuiyuan; Zhou, Jiaojiao; He, Zhijun; Zhang, Na; Ye, Yuanyuan; Liu, Xiaoqing] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Ye, Yuanyuan; Liu, Xiaoqing] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[He, Zhijun] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Biotechnol & Ecol, Shenzhen 518055, Peoples R China.;[Cai, Jie] Hubei Key Lab Nutr Qual & Safety Agro Prod, Wuhan 430064, Peoples R China.
通讯机构：
[Jie Cai] K;[Jiangling He] A;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, China
作者机构：
[Yang, Rundong; Zhu, Zhenzhou; Li, Shuyi; Li, Yuanhong; Zhang, Na; Wang, Feifei] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Zhu, ZZ ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Carbohydrate accumulation;Organic selenium;Selenite;Selenocysteine;Tribonema minus
摘要：
This study aimed to investigate the physiological and biochemical responses of filamentous microalga Tribonema minus to different Na2SeO3 concentrations and its selenium absorption and metabolism to evaluate the potential in treating selenium-containing wastewater. The results showed that low Na2SeO3 concentrations promoted growth by increasing chlorophyll content and antioxidant capacity, whereas high concentrations caused oxidative damage. Although Na2SeO3 exposure reduced lipid accumulation compared with the control, it significantly increased carbohydrate, soluble sugar, and protein contents, with the highest carbohydrate productivity of 117.97 mg/L/d at 0.5 mg/L Na2SeO3. Furthermore, this alga effectively absorbed Na2SeO3 in the growth medium and converted most of it into volatile selenium and a small part into organic selenium (predominantly as selenocysteine), showing strong selenite removal efficacy. This is the first report on the potential of T. minus to produce valuable biomass while removing selenite, providing new insights into the economic feasibility of bioremediation of selenium-containing wastewater.
摘要：
Cyclocarya paliurus (Batal.) lljinskaja, the sole and multi-functional tree species of the family Juglandaceae, grows extensively in subtropical areas of China. Species-specific triterpenoids in its leaves have validated health-promoting effects, including hypoglycemic and hypolipidemic activities. To illustrate the effect of MeJA treatment on the accumulation and biosynthesis of triterpenoids in C. paliurus leaves at different stages of maturity, the contents of total triterpenoids and six triterpene compounds, along with the relative expression of three key genes, were detected. The results showed that the contents of triterpenes and expression patterns of the genes significantly differed among the samples. Different treatment times also had diverse effects on triterpenoid accumulation and gene expression. MeJA treatment had positive effects on total triterpenoids, cyclocaric acid B, and cyclocarioside A/B, especially in young leaves. Gene expression was highest in young leaves after 10 days of treatment, indicating that they were the most sensitive to MeJA. This study provided a reference for improving the accumulation of triterpenoids in C. paliurus plantations in the future.
作者机构：
[Cheng, Shuiyuan; He, Yi; Zhu, Lisha; Dong, Xingxing; Long, Pengcheng; Lai, Huafa; He, Jingren] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Liu, Gang; He, Yi; Zhu, Lisha; Lai, Huafa; Xu, Suyin; He, Jingren] Wuhan Polytech Univ, Sch Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod,Minist E, Wuhan 430023, Peoples R China.;[Shao, Yanchun] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Wang, Liling] Tarim Univ, Coll Life Sci, Alar 843300, Peoples R China.
通讯机构：
[Yi He] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
Monascus red pigments;liposomes;morphological characteristics;stability;anticancer activity
通讯机构：
[Hua Cheng; Li Li] A;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
关键词：
biological nano selenium;nutritional composition;antioxidant stress;organic selenium;ABC transporter family
摘要：
It is an important way for healthy Selenium (Se) supplement to transform exogenous Se into organic Se through crops. In the present study, Vigna unguiculata was selected as a test material and sprayed with biological nano selenium (SeNPs) and Na2SeO3, and its nutrient composition, antioxidant capacity, total Se and organic Se content were determined, respectively. Further, the response of ABC transporter family members in cowpea to different exogenous Se treatments was analyzed by transcriptome sequencing combined with different Se forms. The results show that the soluble protein content of cowpea increased after twice Se treatment. SeNPs treatment increased the content of cellulose in cowpea pods. Na2SeO3 treatment increased the content of vitamin C (Vc) in cowpea pods. Se treatments could significantly increase the activities of Peroxidase (POD), polyphenol oxidase (PPO) and catalase (CAT) in cowpea pods and effectively maintain the activity of Superoxide dismutase (SOD). SeNPs can reduce the content of malondialdehyde (MDA) in pods. After Se treatment, cowpea pods showed a dose-effect relationship on the absorption and accumulation of total Se, and Na2SeO3 treatment had a better effect on the increase of total Se content in cowpea pods. After treatment with SeNPs and Na2SeO3, the Se species detected in cowpea pods was mainly SeMet, followed by MeSeCys. Inorganic Se can only be detected in the high concentration treatment group. Analysis of transcriptome data of cowpea treated with Se showed that ABC transporters could play an active role in response to Se stress and Se absorption, among which ABCB, ABCC and ABCG subfamilies played a major role in Se absorption and transportation in cowpea. Further analysis by weighted gene co-expression network analysis (WGCNA) showed that the content of organic Se in cowpea treated with high concentration of SeNPs was significantly and positively correlated with the expression level of three transporters ABCC11, ABCC13 and ABCC10, which means that the ABCC subfamily may be more involved in the transmembrane transport of organic Se in cells.
