作者机构：
[Chuanlin Hu] State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan, P. R. China;[Pengcheng Wang] Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, P. R. China;[Michael Mak] Department of Biomedical Engineering, School of Engineering & Applied Science, Yale University, New Haven, USA;[Qing Chang] Department of Chemical and Biomolecular Engineering, Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong;[Kun Wang] Division of Biostatistics, Department of Population Health, New York University, New York, USA
通讯机构：
[Zhiyong Gong; Yang Wu] H;Hubei Key Laboratory for Processing and Transformation of Agricultural Products (Wuhan Polytechnic University), College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, P. R. China
摘要：
<jats:p>A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.</jats:p>
摘要：
<jats:title>Abstract</jats:title><jats:p>Exposure to PM2.5 has become one of the most important factors affecting public health in the world. Both clinical and research studies have suggested that PM2.5 inhalation is associated with impaired lung function. In this study, material characterization identified the existence of nanoscale particulate matter (NPM) in airborne PM2.5 samples. When coming into contact with protein-rich fluids, the NPM becomes covered by a protein layer that forms a “protein corona”. Based on a 3D organotypic cell culture, the protein corona was shown to mitigate NPM cytotoxicity and further stimulate the proliferation of human lung fibroblasts (HLFs). ROS-activated alpha-smooth muscle actin (α-SMA) is considered to be one of the proliferation pathways. In this research, 3D cell cultures exhibited more tissue-like properties compared with the growth in 2D models. Animal models have been widely used in toxicological research. However, species differences make it impossible to directly translate discoveries from animals to humans. In this research, the 3D HLF model could partly simulate the biological responses of NPM-protein corona-induced aberrant HLF proliferation in the human lung. Our 3D cellular results provide auxiliary support for an animal model in research on PM2.5-induced impaired lung function, particularly in lung fibrosis.</jats:p>
作者机构：
[Wu, Yang; Li, Yan; Wang, Peng-Cheng; Gong, Zhi-Yong] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Pi, Qing-Meng] MIT, Harvard MIT Div Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA.;[Li, Yan] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;[Pi, Qing-Meng] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthoped Surg, Shanghai 200233, Peoples R China.
通讯机构：
[Wu, Yang] W;[Yang, Xu] C;Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Exposure to ambient fine particulate matter (FPM) has been thought to be associated with cardiovascular disease. However, the pathogenesis remains largely unknown. Animal models have been widely used in toxicological research, but species difference makes it impossible to directly translate discoveries from animals to humans. In this study, we developed a 3D functional human microvascular network in a microfluidic device. The established model enables endothelial cells to form vessel-like microtissues and have physiological functions which are closer to cells in human blood vessels. The perfusable microvasculature allows the delivery of nutrients, and oxygen, as well as flow-induced mechanical stimuli into the luminal space of the endothelium. The microflow effectively mimic the blood flow in human vessels. FPMs were introduced into this physiologically human vessel-like microenvironment following the fluid flow. The vascular toxicity was evaluated based on this organotypic 3D microvessel model. Our results demonstrated that intravascular accumulation of FPM could enhance ROS generation which may further cause endothelial dysfunction by oxidative stress. This is expressed in disorder of NO expression and IL-6 up-regulation. These are expected to enhance endothelial inflammation which might in turn accelerate coagulation that is associated with thrombosis. Human organotypic 3D microvessel models provide a possible bridge for how the research outcomes translate to humans. These models could partly simulate the physiological responses of human vessels to FPM stimulation. This simple and versatile platform can be used for a wide range of applications in vascular physiology studies of particulate matter in the context of cardiovascular disease.
摘要：
Hydrodistillation, solvent, enzymolysis and supercritical fluid extraction (SFE) methods were used to extract the essential oil of Osmanthus fragrans Lour.. The components of the essential oil were analyzed by gas chromatography/mass spectrometry (GC-MS). The yields of essential oil extracted by theose four different methods from O. fragrans Lour were were 0.14%, 0.89%, 0.94%, 1.11% respectively, based on the dry weight. Trans-linalool oxide, cis-linalool oxide, beta-ionone, 9-Tricosene, hexadecanoic acid, 9,12,15-octadecatrienoic acid and 9,12,15-octadecatrienoic acid, ethyl ester were the primary components of O. fragrans essential oil. The chemical analysis revealed that the percentage of those primary components of essential oil extracted by different methods were widely different. The results indicated that supercritical fluid extraction method with suitable extract conditions was more selective than the other methods. SFE is the best method for extracting the essential oil of O. fragrans which was solvent free and available in high purity at relatively low cost in the extraction of essential oil.
摘要：
Article Crystal structure of bis(3,5-dichloro-N-2-(dimethylamino)ethylsalicylaldiminato- κ3N,N',O)manganese(II) monohydrate, Mn(C11H13Cl2N2O)2 · H2O was published on June 1, 2012 in the journal Zeitschrift für Kristallographie - New Crystal Structures (volume 227, issue 2).
作者：
Liu Zhiguo;Fu Yunjie;Li Qi;Li Kui;Chen Jiangyuan;...
期刊：
ITME 2011 - Proceedings: 2011 IEEE International Symposium on IT in Medicine and Education,2011年2:628-632
通讯作者：
Liu, Z.(Zhiguo_1@126.com)
作者机构：
[Liu Zhiguo; Fu Yunjie; Li Qi; Li Kui; Chen Jiangyuan; Zhou Guoping; Li Rui; Liu Lieju] School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
会议名称：
2011 IEEE International Symposium on IT in Medicine and Education
会议时间：
December 2011
会议地点：
Cuangzhou
会议论文集名称：
2011 IEEE International Symposium on IT in Medicine and Education
