通讯机构：
[Zhang, Jibin] H;Huazhong Agr Univ, Coll Life Sci & Technol, Natl Engn Res Ctr Microbial Pesticides, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China.
关键词：
Black soldier fly;Environment;Pollution;Soybean curd residues;Waste management
摘要：
Black soldier fly larvae (BSFL), Hermetia illucens (Diptera: Stratiomyidae) can reduce environmental pollution and convert organic wastes into biomass that is rich in protein and fat. The influence of the nutritional characteristics of organic waste on BSFL characteristics relevant for food and feed safety remains poorly understood. To evaluate the conversion of soybean curd residues (SCR) into high-quality animal-derived proteins and fats for human and livestock consumption, this study assessed the co-conversion efficacy, nutrient composition, safety, and anti-nutritional factor concentrations in BSFL after the development on SCR with Lactobacillus buchneri (L3-9). SCR was pretreated with L. buchneri (10(8) cfu/ml), and then BSFL was employed for conversion. BSFL fed with SCR and L. buchneri had a significantly higher dry mass reduction (55.7+/-0.9%), bioconversion rate (6.9+/-0.3%), crude protein content (55.3+/-0.6%), and fat content (30.0+/-0.6%) than SCR (49.0+/-0.7%, 5.0+/-0.3%, 52.8+/-0.3%, and 26.1+/-0.8%, respectively) and artificial feed (43.9+/-0.8%, 3.9+/-0.1%, 50.3+/-0.4%, and 24.3+/-0.4%, respectively). However, the feed conversion ratio (8.0+/-0.3), of BSFL fed with SCR and L. buchneri was lower than that of the BSFL fed with SCR (9.8+/-0.1) and artificial feed (11.1+/-0.5). In addition, BSFL had satisfactory concentrations of all essential amino acids and fatty acids required for human consumption as recommended by WHO/FAO/UNU. The heavy metals and anti-nutritional factor concentrations were within the safety intake levels for food and feedstock. Therefore, the addition of L. buchneri with BSFL on SCR did not only increase co-conversion performance but also enhanced the nutritional value of BSFL.
作者机构：
[Gao TuLing; Zhao Gong; Chen Cheng; He XinYi; Deng ZiXin] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200030, Peoples R China.;[Gao TuLing; Zhao Gong; Chen Cheng; He XinYi; Deng ZiXin] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200030, Peoples R China.;[Gao TuLing; Hu ShenCai] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Xu, H. Howard] Calif State Univ Los Angeles, Dept Biol Sci, Los Angeles, CA 90032 USA.
通讯机构：
[He XinYi] S;Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200030, Peoples R China.
摘要：
The thiol-functionalized eggshell membrane (TF-ESM) was prepared by esterification between thioglycolic acid (CH2SHCOOH) and oxygen-containing functional groups on eggshell membrane (ESM). A promising potential biosorbent, TF-ESM, was used for the removal of toxic mercury in wastewater. Aqueous of mercury ions removing properties by TF-ESM were studied through batch experiments. The results indicated that the modified ESM has greatly enhanced the adsorption capacity for Hg2+, the maximum capacity was increased more than 3-fold in comparison with the unmodified ESM, from 19.4 mg g-1 to 71.9 mg g-1. The optimum of pH value for Hg adsorption was in the range of 2-8, which the removing rate over 96%. The results obtained show that the novel thiol-functionalized eggshell membrane performed well the removal and recovery of mercury from low concentration wastewaters.
作者机构：
[庄令; 闫莉萍; 洪葵] Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricuture Sciences, Haikou 571101, China;[谭仁祥] Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China;Department of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[胡申才] Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricuture Sciences, Haikou 571101, China, Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China, Department of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Hu, S.-C.] I;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricuture Sciences, China
摘要：
A marine Streptomyces sp. 060524 capable of hydrolyzing the glycosidic bond of isoflavone glycosides, was isolated by detecting its β-glucosidase activity. 5 isoflavone aglycones were isolated from culture filtrates in soybean meal glucose medium. They were identified as genistein (1), glycitein (2), daidzein (3), 3′,4′,5,7-tetrahydroxyisoflavone (4), and 3′,4′,7-trihydroxyisoflavone (5), based on UV, NMR and mass spectral analysis. The Streptomyces can selectively hydroxylate at the 3′-position in the daidzein and genistein to generate 3′-hydroxydaidzein and 3′-hydroxygenistein, respectively. The Strain biotransformed more than 90% of soybean isoflavone glycosides into their aglycones within 108 h. 3′-hydroxydaidzein and 3′-hydroxygenistein exhibited stronger cytotoxicity against K562 human chronic leukemia than daidzein and genistein.
