Microarray analysis reveals the inhibition of intestinal expression of nutrient transporters in piglets infected with porcine epidemic diarrhea virus.
作者：
Zhang, Junmei;Zhao, Di;Yi, Dan;Wu, Mengjun;Chen, Hongbo;...
期刊：
Scientific Reports ,2019年9(1):19798 ISSN：2045-2322
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Zhou, Jia; Hou, Yongqing; Wu, Mengjun; Li, Peng; Zhao, Di; Zhang, Junmei; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
摘要：
Porcine epidemic diarrhea virus (PEDV) infection can induce intestinal dysfunction, resulting in severe diarrhea and even death, but the mode of action underlying these viral effects remains unclear. This study determined the effects of PEDV infection on intestinal absorption and the expression of genes for nutrient transporters via biochemical tests and microarray analysis. Sixteen 7-day-old healthy piglets fed a milk replacer were randomly allocated to one of two groups. After 5-day adaption, piglets (n = 8/group) were orally administrated with either sterile saline or PEDV (the strain from Yunnan province) at 10(4.5) TCID50 (50% tissue culture infectious dose) per pig. All pigs were orally infused D-xylose (0.1 g/kg BW) on day 5 post PEDV or saline administration. One hour later, jugular vein blood samples as well as intestinal samples were collected for further analysis. In comparison with the control group, PEDV infection increased diarrhea incidence, blood diamine oxidase activity, and iFABP level, while reducing growth and plasma D-xylose concentration in piglets. Moreover, PEDV infection altered plasma and jejunal amino acid profiles, and decreased the expression of aquaporins and amino acid transporters (L-type amino acid transporter 1, sodium-independent amino acid transporter, B( degrees (,+))-type amino acid transport protein, sodium-dependent neutral amino acid transporter 1, sodium-dependent glutamate/aspartate transporter 3, and peptide transporter (1), lipid transport and metabolism-related genes (lipoprotein lipase, apolipoprotein A1, apolipoprotein A4, apolipoprotein C2, solute carrier family 27 member 2, solute carrier family 27 member 4, fatty acid synthase, and long-chain acyl-CoA synthetase (3), and glucose transport genes (glucose transporter-2 and insulin receptor) in the jejunum. However, PEDV administration increased mRNA levels for phosphoenolpyruvate carboxykinase 1, argininosuccinate synthase 1, sodium/glucose co-transporter-1, and cystic fibrosis transmembrane conductance regulator in the jejunum. Collectively, these comprehensive results indicate that PEDV infection induces intestinal injury and inhibits the expression of genes encoding for nutrient transporters.
语种：
英文
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幼龄仔猪PEDV感染肠道损伤模型的建立
作者：
李鹏;吴梦郡;余魁;徐海旺;吴涛;...
期刊：
中国畜牧兽医 ,2018年45(6):1715-1721 ISSN：1671-7236
作者机构：
[余魁; 李鹏; 侯永清; 徐海旺; 吴梦郡; 王蕾; 易丹; 赵迪; 吴涛] 武汉轻工大学
关键词：
猪流行性腹泻病毒;仔猪;肠道损伤;模型
摘要：
试验旨在建立猪流行性腹泻病毒（PEDV）感染幼龄仔猪肠道损伤模型。试验选用16头7日龄健康幼龄仔猪（杜×长×大）,随机分为两个处理组:对照组和PEDV组,每个处理8个重复,每个重复1头猪。试验期为10d,试验期间两个处理组饲喂相同的基础日粮。于试验第7天晚上对PEDV组仔猪口腔灌服PEDV病毒（剂量为104.5 TCID50）,对照组灌服等量的生理盐水。于试验第10天早上空腹灌服D-木糖（0.1g/kg体重）,1h后前腔静脉采血,屠宰取样,取十二指肠、空肠、回肠等组织样品,测定平均日增重（ADG）、腹泻率（DR）、肠道形态结构、肠道黏膜损伤相关基因mRNA水平。试验结果表明:（1）PEDV感染显著降低了仔猪ADG（P<0.05）,极显著提高了仔猪腹泻率（P<0.01）;（2）PEDV感染极显著降低了血浆D-木糖含量、空肠和回肠绒毛高度、十二指肠、空肠和回肠绒毛高度与隐窝深度的比值（P<0.01）,显著提高了十二指肠和空肠隐窝深度（P<0.05）;（3）PEDV感染极显著提高了仔猪空肠黏膜PEDV M基因的相对表达量（P<0.01）,极显著降低了肠绒毛蛋白（villin）和肠型脂肪酸结合蛋白（i-FABP）基因的相对表达量（P<0.01）。以上结果表明,口腔灌服PEDV可以成功诱导建立幼龄仔猪肠道损伤模型。
语种：
中文
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凝结芽孢杆菌对断奶仔猪生长性能、肠道菌群和血液生化指标的影响
作者：
张越;徐海旺;李鹏;周颖;张俊梅;...
期刊：
黑龙江畜牧兽医 ,2018年(12):165-168 ISSN：1004-7034
作者机构：
武汉轻工大学动物科学与营养工程学院/动物营养与饲料科学湖北省重点实验室,武汉,430023;[李鹏; 侯永清; 徐海旺; 张俊梅; 张越; 周颖; 赵迪] 武汉轻工大学
关键词：
凝结芽孢杆菌;断奶仔猪;生长性能;肠道菌群;血液生化指标
摘要：
为了研究日粮中添加凝结芽孢杆菌对仔猪生长性能、肠道菌群和血液生化指标的影响,试验选取21日龄健康断奶仔猪30头,随机分为3组(对照组、试验Ⅰ组、试验Ⅱ组),对照组饲喂基础日粮,试验Ⅰ组、试验Ⅱ组分别在基础日粮中添加凝结芽孢杆菌2.0× 106 cfu/g、2.0× 107 cfu/g.预试期3d,正试期21 d.于试验第21天采血,屠宰,测定其生长性能、肠道菌群和血液生化指标.结果表明:日粮中添加不同水平的凝结芽孢杆菌均显著降低了断奶仔猪的腹泻率(P＜0.05);提高了仔猪回肠内容物中乳酸菌的数量(P＜0.05),降低了回肠内容物中梭菌的数量(P＜0.05);提高了血浆中磷酸丝氨酸、胱氨酸、酪氨酸等多种氨基酸的含量(P＜0.05),降低了磷酰乙醇铵的含量(P＜0.05).说明在日粮中添加凝结芽孢杆菌可以改善断奶仔猪的肠道菌群,降低仔猪的腹泻率.
语种：
中文
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复合植物精油对脂多糖诱导的断奶仔猪免疫应激的影响
作者：
徐海旺;余魁;吴梦郡;刘文凯;李鹏;...
期刊：
中国畜牧兽医 ,2018年45(8):2197-2203 ISSN：1671-7236
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉 430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心 ,武汉430023;[余魁; 李鹏; 侯永清; 徐海旺; 吴梦郡; 王蕾; 易丹; 刘文凯] 武汉轻工大学
关键词：
复合植物精油(OCT);断奶仔猪;脂多糖(LPS);免疫应激
摘要：
试验旨在研究复合植物精油(OC T)对脂多糖(L PS)诱导的断奶仔猪的免疫应激的影响.试验选取28日龄左右的杜×长×大三元杂交仔猪,随机分成3个处理组:对照组(基础日粮,注射生理盐水),L PS组(基础日粮,注射LPS),OCT组(基础日粮+50 mg/kg OCT,注射LPS).每组8个重复,每个重复1头猪.于试验第21天注射L PS(100μg/kg体重)或等体积的生理盐水,注射3 h后前腔静脉采血进行白细胞分类计数;6 h后屠宰,取胸腺、脾脏,-80℃保存,用于测量胸腺、脾脏免疫相关基因白介素-4(IL-4)、白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子(TNF-α)、核因子(NF-κB)以及转录相关基因转录激活因子3(STAT3)的基因相对表达量.结果表明:①与对照组相比,L PS刺激降低了仔猪血液中白细胞、中性粒细胞、淋巴细胞、单核细胞及嗜酸性粒细胞数量(P<0.05);与LPS组相比,日粮中添加OCT提高了白细胞的数量(P<0.05);②与对照组相比,LPS刺激上调了脾脏、胸腺IL-6、STAT3 mRNA水平(P<0.05),下调了脾脏IL-4、TNF-α及胸腺IL-4、TNF-α、NF-κB mRNA水平(P<0.05);与LPS组相比,添加OCT下调了脾脏、胸腺IL-6、STAT3 mRNA水平(P<0.05);上调了胸腺IL-4、IL-10、TNF-α、NF-κB的mRNA水平(P<0.05).综合上述结果,LPS诱导发生了仔猪免疫应激,OCT在一定程度具有缓解作用.
语种：
中文
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N-乙酰半胱氨酸灌胃对超早期断奶仔猪血浆中游离氨基酸及小肠形态结构与功能的影响
作者：
周佳;李鹏;程强;张俊梅;周颖;...
期刊：
中国畜牧杂志 ,2018年54(4):50-54 ISSN：0258-7033
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[李鹏; 周佳; 侯永清; 张俊梅; 程强; 王蕾; 周颖; 易丹] 武汉轻工大学
关键词：
N-乙酰半胱氨酸;仔猪;游离氨基酸;小肠
摘要：
本试验旨在研究N-乙酰半胱氨酸(N-acetylcysteine,NAC)灌胃对超早期断奶仔猪血浆中游离氨基酸、小肠形态结构及功能的影响.将22头体重为(2.99±0.41) kg的7日龄断奶仔猪随机分为对照组和NAC组,每组1 1个重复,每个重复1头猪.在饲喂人工乳的基础上,NAC组每天每头按50 mg/kg体重进行NAC灌胃,对照组每天每头进行同体积的生理盐水灌胃.于试验第11天按0.1 g/kg体重的剂量给仔猪灌服D-木糖溶液,1h后前腔静脉采血,然后屠宰取小肠组织样,刮取肠黏膜及制作组织切片.结果表明:与对照组相比,NAC组显著提高了超早期断奶仔猪平均日增重(P＜0.05);提高了超早期断奶仔猪血浆中谷氨酰胺、胱氨酸、γ-氨基丁酸水平(P＜0.05),降低了血浆中尿素氮、半胱氨酸、色氨酸水平和二胺氧化酶活力(P＜0.05):NAC灌胃提高了哺乳仔猪十二指肠黏膜的绒毛高度/隐窝深度(P＜0.05),有降低十二指肠隐窝深度的趋势(P＜0.10),有提高空肠绒毛宽度和回肠绒毛高度的趋势(P＞0.10).在本试验条件下,NAC灌胃可改善小肠形态结构与屏障功能,提高氨基酸的利用率,调节血浆中部分氨基酸的含量.
语种：
中文
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复合植物精油对脂多糖刺激仔猪肝脏的保护作用
作者：
李鹏;汪中兴;吴梦郡;余魁;纪昌正;...
期刊：
中国畜牧杂志 ,2018年54(3):87-91 ISSN：0258-7033
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[余魁; 李鹏; 侯永清; 徐海旺; 吴梦郡; 王蕾; 汪中兴; 易丹; 赵迪; 纪昌正] 武汉轻工大学
关键词：
复合植物精油;脂多糖;仔猪;肝脏
摘要：
试验旨在研究复合植物精油（OCT）对脂多糖（LPS）刺激仔猪肝脏的保护作用。选取18头仔猪,随机分为对照组、LPS组和OCT+LPS组,每组6个重复。对照组与LPS组饲喂基础日粮,OCT+LPS组在基础日粮中添加50 mg/kg OCT。试验期21 d。于试验第21天,LPS组与OCT+LPS组仔猪腹腔注射LPS,对照组注射等量的生理盐水,3 h后采血,6 h后屠宰。结果表明:与对照组相比,LPS刺激提高了血浆谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、谷酰转肽酶（GGT）、肝脏诱导型一氧化氮合酶（i-NOS）的活力（P<0.05）,肝脏热休克蛋白70（HSP70）基因的相对表达量显著提高（P<0.05）;仔猪肝脏表皮生长因子（EGF）、白细胞介素10（IL-10）、胰岛素样因子（IGF-1）、丝氨酸蛋白激酶（mTOR）基因的相对表达量显著降低（P<0.05）。在日粮中添加50 mg/kg的OCT,可缓解LPS导致的仔猪血浆ALT、AST、GGT活力的升高以及肝脏i-NOS活力的升高（P<0.05）,且有缓解LPS导致的仔猪肝脏MPO活力升高的趋势（P<0.1）,另外可缓解LPS导致的仔猪肝脏HSP70基因相对表达量的升高以及EGF、IL-10、IGF-1、mTOR等基因相对表达量的降低（P<0.05）。综上可知,日粮中添加50 mg/kg的OCT可缓解LPS刺激引起的肝脏炎症反应,提高仔猪肝脏细胞的生长和增殖,进而缓解LPS刺激导致的仔猪肝脏氧化应激损伤。
语种：
中文
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Establishment of a recombinant Escherichia coli-induced piglet diarrhea model
作者：
Wu, Tao;Lv, Yang;Li, Xueni;Zhao, Di;Yi, Dan;...
期刊：
FRONTIERS IN BIOSCIENCE-LANDMARK ,2018年23(8):1517-1534 ISSN：2768-6701
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Gong, Joshua; Lv, Yang; Hou, Yongqing; Wang, Lei; Li, Xueni; Li, Peng; Wu, Guoyao; Zhao, Di; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Intestinal injury;Piglet Diarrhea;Recombinant Escherichia coli;Review
摘要：
Enterotoxigenic Escherichia coli strains induce human and animal intestinal dysfunction and injury, and cause diarrhea in weanling pigs. A recombinant E.coli strain LMG194-STa which expressed single toxin STa of ETEC was constructed by directly inserting the STa gene of ETEC into the expression vector pBAD202 and then transferring the recombinant plasmid pBAD-STa into the E.coli LMG19 host strain. Diarrhea and intestinal injury in piglets were induced by oral administration of recombinant strain LMG194-Sta. In vitro, the recombinant strain LMG194-Sta had the same toxicity to the IPEC cell as wild type strain K88, and higher toxicity than the host strain LMG194. In vivo, LMG194-STa caused severe diarrhea in piglets as K88, the diarrhea rate of LMG194-STa and K88 groups was higher than that in the LMG194 and control groups. Both K88 and LMG194-STa induced intestinal inflammation, injury, and atrophy, while adversely affecting the expression of genes for cytokines, transporters and ion channels, and nutrient metabolism. Thus, we established a porcine model of recombinant E. coli strain LMG194-STa-induced diarrhea for future nutritional and mechanistic studies of intestinal dysfunction. © 2018 Frontiers in Bioscience. All Rights Reserved.
语种：
英文
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Dietary supplementation with lactobacillus casei alleviates lipopolysaccharide-induced liver injury in a porcine model
作者：
Zhao, Di;Wu, Tao;Yi, Dan;Wang, Lei;Li, Peng;...
期刊：
International Journal of Molecular Sciences ,2017年18(12):2535 ISSN：1661-6596
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Hou, Yongqing; Wang, Lei; Li, Peng; Wu, Guoyao; Zhao, Di; Yi, Dan; Zhang, Junmei] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
acidifying agent;alanine aminotransferase;aspartate aminotransferase;calcium;calcium propionate;catalase;choline;gamma glutamyltransferase;glutathione peroxidase;heat shock protein 70;immunoglobulin enhancer binding protein;interleukin 6;interleukin 8;limestone;lipopolysaccharide;lysine;malonaldehyde;methionine;phosphorus;protein;sodium chloride;soybean protein;superoxide dismutase;threonine;toll like receptor 4;tryptophan;tumor necrosis factor;gamma glutamyltransferase;heat shock protein 70;immunoglobulin enhancer binding protein;interleukin 6;lipopolysaccharide;malonaldehyde;probiotic agent;tumor necrosis factor;animal experiment;animal model;animal tissue;Article;bleeding;cell vacuole;controlled study;diarrhea;diet supplementation;energy;enzyme activity;enzyme assay;experimental liver injury;female;fish meal;gene expression;histopathology;Lactobacillus casei;liver histology;liver sinusoid;lymphocytic infiltration;maize;nonhuman;performance;porcine model;reverse transcription polymerase chain reaction;soybean;ultraviolet spectrophotometry;wheat;whey;animal;blood;dietary supplement;liver;liver disease;metabolism;pig;Animals;Dietary Supplements;Female;gamma-Glutamyltransferase;HSP70 Heat-Shock Proteins;Interleukin-6;Lactobacillus casei;Lipopolysaccharides;Liver;Liver Diseases;Malondialdehyde;NF-kappa B;Probiotics;Swine;Tumor Necrosis Factor-alpha
摘要：
This study aims to determine whether Lactobacillus casei (L. casei) could relieve liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were randomly allocated into one of the three groups: control, LPS, and L. casei. The control and LPS groups were fed a corn- and soybean meal-based diet, whereas the L. casei group was fed the basal diet supplemented with 6 × 106 cfu/g L. casei. On Day 31 of the trial, piglets in the LPS and L. casei groups receivedintraperitoneal administration of LPS (100 μg/kg body weight), while the control group received the same volume of saline. Blood and liver samples were collected for analysis. Results showed that L. casei supplementation decreased the feed/gain ratio (p = 0.027) and diarrhea incidence (p < 0.001), and attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, LPS challenge dramatically increased glutamyl transpeptidase activity (p = 0.001) in plasma as well as the concentrations of Interleukin 6 (IL-6) (p = 0.048), Tumor necrosis factor-alpha (TNF-α) (p = 0.041), and Malondialdehyde (MDA) (p = 0.001) in the liver, while decreasing the hepatic SOD activity. LPS also increased (p < 0.05) the mRNA levels for IL-6, IL-8, TNF-α, Toll-like receptors 4 (TLR4), Nuclear factorkB (NF-kB) and Heat shock protein 70 (HSP70) in the liver. The adverse effects of LPS challenge were ameliorated by L. casei supplementation. In conclusion, dietary L. casei alleviates LPS-induced liver injury via reducing pro-inflammatory cytokines and increasing anti-oxidative capacity. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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L-亮氨酸对猪流行性腹泻病毒感染仔猪血液生化指标和空肠免疫相关基因mRNA水平的影响
作者：
谢佳倩;周颖;张俊梅;徐海旺;李鹏;...
期刊：
中国饲料 ,2017年(12):27-31 ISSN：1004-3314
作者机构：
动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室,武汉轻工大学动物科学与营养工程学院,湖北武汉 430023;[李鹏; 侯永清; 徐海旺; 张俊梅; 王蕾; 周颖; 易丹; 赵迪; 谢佳倩] 武汉轻工大学
关键词：
亮氨酸;仔猪;猪流行性腹泻病毒;血液生化指标;免疫
摘要：
本试验旨在研究L-亮氨酸（Leu）对猪流行性腹泻病毒（PEDV）感染幼龄仔猪血液指标和空肠免疫相关基因m RNA表达量的影响,探索Leu对PEDV感染仔猪应激的缓解作用。选用30头体重相近的7日龄PEDV阴性仔猪,随机分为3个处理组：对照组、PEDV组和PEDV＋Leu组,每个处理10个重复,每个重复一头猪。试验期10 d（7日龄~16日龄）。从第0天开始对PEDV＋Leu组按2.8 g/kg体重饲喂Leu,而对照组和PEDV组均按4.1 g/kg体重饲喂L-丙氨酸。第7天早上对PEDV组和PEDV＋Leu组采用口服的方式接种106.5TCID50的PEDV,同时对照组采用口服的方式接种等剂量的生理盐水,3 d后（16日龄）取血液和肠道样品。结果表明：与对照组相比,PEDV组仔猪血液中总胆红素（TBIL）水平、血小板分布宽度（PDW）及空肠黏膜CXCL11、REG3G m RNA表达量分别降低了20.92%、9.76%、56.00%、36.00%（P〈0.05）,血液中白细胞（WBC）和淋巴细胞（LYM）数量、磷酸丝氨酸、酪氨酸和色氨酸浓度,及空肠粘膜GSTO2和HSPH1 m RNA水平分别提高了61.68%、27.66%、30.39%、50.54%、64.00%、75.00%、89.00%（P〈0.05）;与PEDV组相比,PEDV＋Leu组仔猪血液中组氨酸、天冬酰胺、尿素氮（BUN）浓度分别升高了55.43%、32.58%、83.33%（P〈0.05）,而二胺氧化酶（DAO）含量、单核细胞（MONO）、单核细胞比（Monocyte Ratio,MONOR）、磷酸丝氨酸、谷氨酸、异亮氨酸、精氨酸、苏氨酸及空肠黏膜MX1和GSTO2 m RNA水平分别降低了28.80%、47.13%、43.87%、10.53%、42.12%、29.48%、29.82%、33.50%、32.11%、28.00%（P〈0.05）。因此,日粮中添加Leu提高了PEDV感染仔猪的免疫功能,缓解了PEDV感染仔猪的应激。
语种：
中文
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N-乙酰半胱氨酸对7日龄感染猪流行性腹泻病毒仔猪肠道吸收功能的影响
作者：
周佳;李鹏;侯永清;王蕾;张越;...
作者机构：
[周佳; 李鹏; 侯永清; 王蕾; 张越; 谢佳倩; 徐海旺] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
N-乙酰半胱氨酸;猪流行性腹泻病毒;仔猪;肠道吸收
摘要：
猪流行性腹泻(PED)是由于猪感染流行性腹泻病毒(PEDV)后,导致猪产生呕吐、高度腹泻及高致死等症状的消化道传染病。猪感染PEDV后,其小肠绒毛会受到损伤、并且使其消化和吸收功能发生紊乱,机体的稳态会遭到破坏,使猪出现高度腹泻甚至导致其死亡。N-乙酰半胱氨酸(NAC)作为功能性氨基酸被证实可以改善肠道功能,对肠道感染有很好的保护作用。本试验旨在以7日龄仔猪为研究对象,探讨NAC对流行性腹泻病毒感
语种：
中文
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短链脂肪酸酯对仔猪生长性能、肠道功能的影响及作用机制
作者：
李抗;张越;李鹏;王蕾;侯永清;...
作者机构：
[李抗; 张越; 李鹏; 王蕾; 侯永清; 丁斌鹰; 易丹] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
己酸甘油三酯;乳酸甘油三酯;仔猪;生长性能;肠道功能
摘要：
本试验旨在研究短链脂肪酸酯对仔猪生长性能、肠道功能的作用及机制。选取24头(21±2)天健康的断奶仔猪,随机分成3个处理组(每组8个重复,每个重复1头仔猪):对照组、己酸甘油三酯组和乳酸甘油三酯组;对照组仔猪饲喂基础日粮,己酸甘油三酯组和乳酸甘油三酯组仔猪在基础日粮上分别添加0.5%的己酸甘油三酯和乳酸甘油三酯,试验期21天。于试验第21天采血,屠宰取样。结果表明:1)饲粮添加0.5%的己酸甘油三
语种：
中文
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凝结芽孢杆菌对断奶仔猪生长性能、血液指标及肠道形态结构的影响
作者：
张越;侯永清;王蕾;李鹏;徐海旺
作者机构：
[张越; 侯永清; 王蕾; 李鹏; 徐海旺] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
凝结芽孢杆菌;断奶仔猪;生长性能;血液指标;肠道结构
摘要：
益生菌可抑制机体中有害菌生长,促进优势菌群增殖,增强非特异性免疫功能,从而促进动物生长、增强机体免疫力、提高饲料转化率,产生与抗生素相似的作用效果。本试验首先通过牛津杯法比较了凝结芽孢胞杆菌、地衣芽孢杆菌、屎肠球菌、干酪乳杆菌对大肠杆菌K88的抑菌作用,发现凝结芽孢杆菌较其他三株菌抑菌能力更好。然后以断奶仔猪为研究对象,研究了日粮中添加凝结芽孢杆菌对断奶仔猪生长性能、血液生化指标及肠道形态结构的影
语种：
中文
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Molecular cloning, distribution and ontogenetic expression of the oligopeptide transporter PepT1 mRNA in Tibetan suckling piglets
作者：
Wang, Wence;Shi, Changyou;Zhang, Jianshe;Gu, Wanting;Li, Tiejun;...
期刊：
Amino Acids ,2009年37(4):593-601 ISSN：0939-4451
通讯作者：
Chu, Wuying
作者机构：
[Huang, Ruilin; Chu, Wuying; Li, Peng; Gen, Meimei; Li, Tiejun; Yin, Yulong; Shi, Changyou; Wang, Wence; Gu, Wanting] Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr Physiol & Metab Proc, Changsha 410125, Hunan, Peoples R China.;[Zhang, Jianshe; Chu, Wuying] Changsha Univ, Dept Bioengn & Environm Sci, Changsha 410003, Hunan, Peoples R China.;[Liu, Yulan; Hou, Yongqing; Yin, Yulong] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Chu, Wuying] Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr Physiol & Metab Proc, POB 10, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Chu, Wuying] C;Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr Physiol & Metab Proc, POB 10, Changsha 410125, Hunan, Peoples R China.
关键词：
Amino acid transporter;PepT1;Tibetan pig;Expression
摘要：
The gene encoding the oligopeptide transporter PepT1 (HGMW-approved gene symbol SLC15A1) from Tibetan porcine intestine was cloned. The open reading frame of this cDNA encodes 708 deduced amino acid residues that show high sequence similarity with its ovine and bovine counterparts. The putative protein has 12 putative transmembrane domains, including many structural features that are highly conserved among the vertebrate orthologs. PepT1 mRNA expression can be detected in duodenum, jejunum and ileum from Tibetan pigs at 28 days by RT-PCR. Real-time PCR analysis indicated that the jejunum had the highest expression of PepT1 when compared with the duodenum and ileum. PepT1 mRNA expression in the duodenum and proximal jejunum increases continuously from day 1 to day 14: expression was highest at day14 (P < 0.01) and then decreased gradually from day 21 to day 35. Our findings show that PepT1 mRNA expression in the distal jejunum increased gradually with age in suckling Tibetan piglet, and this may have important implications for amino acid and protein nutrition in young animals.
语种：
英文
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Catabolism of nutritionally essential amino acids in developing porcine enterocytes
作者：
Chen, Lixiang;Li, Peng;Wang, Junjun;Li, Xilong;Gao, Haijun;...
期刊：
Amino Acids ,2009年37(1):143-152 ISSN：0939-4451
通讯作者：
Wu, Guoyao
作者机构：
[Chen, Lixiang; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410128, Hunan, Peoples R China.;[Chen, Lixiang] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Chen, Lixiang; Li, Xilong; Gao, Haijun; Wang, Junjun; Li, Peng; Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wang, Junjun] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Hou, Yongqing] Wuhan Polytech Univ, Dept Feeds Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Wu, Guoyao] T;Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
关键词：
Amino acids;Catabolism;Enterocytes;Pigs
摘要：
This study was conducted using the piglet model to test the hypothesis that mucosal cells of the neonatal small intestine can degrade nutritionally essential amino acids (EAA). Enterocytes were isolated from the jejunum of 0-, 7-, 14-, and 21-day-old pigs, and incubated for 45 min in Krebs buffer containing plasma concentrations of amino acids and one of the following L-[1-14C]- or L-[U-14C]-amino acids plus unlabeled tracees at 0.5, 2, or 5 mM: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine. In these cells, branched-chain amino acids (BCAA) were extensively transaminated and 15-50% of decarboxylated branched-chain α-ketoacids (BCKA) were oxidized to CO2 depending on the age of piglets. BCAA transamination increased but their decarboxylation decreased between 0 and 14 days of age. Addition of 1 and 2 mM α-ketoglutarate to incubation medium dose-dependently stimulated BCAA transamination without affecting their decarboxylation. Western blot analysis revealed that the abundance of mitochondrial BCAA aminotransferase declined but cytosolic BCAA aminotransferase increased between 0 and 14 days of age, with the cytosolic protein being the major isoform in 7- to 21-day-old pigs. BCKA dehydrogenase protein existed primarily as the phosphorylated (inactive) form in enterocytes of newborn pigs and its levels were markedly reduced in older pigs. All measured parameters of BCAA metabolism did not differ between 14- and 21-day-old pigs. In contrast to BCAA, catabolism of methionine and phenylalanine was negligible and that of other EAA was absent in enterocytes from all ages of piglets due to the lack of key enzymes. These results indicate that enterocytes are an important site for substantial degradation of BCAA but not other EAA in the neonatal gut. © 2009 Springer-Verlag.
语种：
英文
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Dietary supplementation with Acanthopanax senticosus extract enhances gut health in weanling piglets
作者：
Fang, Jun;Yan, F. Y.;Kong, X. F.;Ruan, Z.;Liu, Z. Q.;...
期刊：
Livestock Science ,2009年123(2-3):268-275 ISSN：1871-1413
通讯作者：
Yin, Y. L.
作者机构：
[Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410125, Hunan, Peoples R China.;[Liu, Z. Q.; Zhang, Y. Z.; Yan, F. Y.; Yang, F.; Wu, G. Y.; Kong, X. F.; Huang, R. L.; Geng, M. M.; Li, T. J.; Yin, Y. L.; Li, Peng] Chinese Acad Sci, Lab Anim Nutr Physiol & Metab Proc, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Kong, X. F.; Yin, Y. L.; Ruan, Z.] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, G. Y.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Yin, Y. L.] C;Chinese Acad Sci, Lab Anim Nutr Physiol & Metab Proc, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.
关键词：
Bacillus subtilis;Bacteria (microorganisms);Clostridium;Eleutherococcus senticosus;Escherichia coli;Lactobacillus amylovorus;Lactobacillus salivarius;Ruminococcus;Salmonella typhimurium;Staphylococcus aureus
摘要：
The present study was conducted to investigate the effects of Acanthopanax senticosus extract (ASE) as a dietary additive on gut health in weanling piglets by examining diarrhea frequency, intestinal microbiota and morphology. A total of 96 Duroc× (Landrace × Yorkshire) piglets weaned at 21days of age with an average initial body weight (BW) of 5.6 ± 0.4kg were randomly assigned to 3 treatment groups with 4 duplicates of 8 piglets each. The piglets were fed basal diet to which had been added 0 or 1g/kg of ASE, or 0.7g/kg antibiotics, respectively. Fecal consistence was monitored twice daily and the frequency of diarrhea was calculated. On day 21 after the initiation of supplementation, 8 piglets were randomly selected from each treatment group (2 piglets per pen) and slaughtered. The jejunum, ileum, colon and cecum were then excised and fixed in 10% neutral formalin solution to determine villus height and crypt depth, after their contents were collected to determine microbiota. The results showed that dietary supplementation with ASE increased (P < 0.05) the density of bacterial populations that co-migrated with Lactobacillus amylovorus, Lactobacillus salivarius, Bacillus subtilis, and Clostridium lituseburens, but decreased (P < 0.05) those co-migrating with Staphylococcus aureus, Salmonella typhimurium, Ruminococcus forques, and E. coli O157:H7 in the PCR-DGGE profiling analysis when compared with the control group. The villus height of the duodenum, jejunum and ileum increased (P < 0.05) by 14.8, 13.7 and 10.0%, while the crypt depth decreased (P < 0.05) by 17.9, 9.1 and 12.1%, respectively, in response to dietary ASE supplementation. Additionally dietary supplementation with ASE or an antibiotic decreased (P < 0.05) the frequency of diarrhea by 55.6 and 52.2%, respectively, compared with the control group. In conclusion, these findings suggest that dietary supplementation with ASE could regulate the microbiota composition and maintain a normal morphology of gut mucosa in weanling piglets, thereby decreasing diarrhea that resulted from weaning stress. © 2008 Elsevier B.V. All rights reserved.
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英文
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Acanthopanax senticosus extract as a dietary additive enhances the apparent ileal digestibility of amino acids in weaned piglets
作者：
Kong, X. F.;Yin, F. G.;He, Q. H.;Liu, H. J.;Li, T. J.;...
期刊：
Livestock Science ,2009年123(2-3):261-267 ISSN：1871-1413
通讯作者：
Yin, Y. L.
作者机构：
[Deng, Z. Y.; Xie, M. Y.; Kong, X. F.; Yin, Y. L.; Ruan, Z.; Xiong, H.] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China.;[Fan, M. Z.; Kong, X. F.; Huang, R. L.; Liu, H. J.; Yin, F. G.; He, Q. H.; Li, T. J.; Yin, Y. L.; Li, Peng] Chinese Acad Sci, Lab Anim Nutr Physiol & Metab Proc, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Fan, M. Z.] Univ Guelph, Guelph, ON N1G 2W1, Canada.;[Hou, Y. Q.; Liu, Y. L.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hou, Y. Q.; Liu, Y. L.] Wuhan Polytech Univ, Dept Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Yin, Y. L.] N;Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China.
关键词：
Eleutherococcus senticosus;Glycine max;Zea mays
摘要：
The present study was conducted to determine the effects of Acanthopanax senticosus (AS) extract as a dietary additive on serum contents and apparent ileal digestibility (AID) of amino acids (AA) in weaned piglets. In Exp. 1, 60 piglets with an average body weight (BW) of 5.64kg were randomly assigned into 3 treatment groups who received maize-soybean-based diets supplemented with 0 or 1g/kg of AS extract or 0.2g/kg of colistin (n = 20 in each group). Blood samples were randomly collected from 5 piglets per group on days 7, 14 and 28 after the initiation of supplementation to determine the serum contents of free AA. In Exp. 2, 12 barrows with an average initial BW of 7.64kg were also randomly assigned into 3 dietary treatment groups after being surgically fitted with a simple T-cannula at the terminal ileum. Samples of terminal ileal digesta were collected on day 7 for analysis of the AID of AA. The data showed that the serum contents and AID of most AA in the AS extract-supplemented group gradually increased by 15.3-80.8% and 4.1-30.8%, respectively, as the experiment proceeded in comparison with the colistin-supplemented group and/or control group. In conclusion, these findings indicated that AS extract could enhance the digestion and absorption of AA, which may be a potential mechanism of growth promotion. © 2008.
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英文
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