摘要:
Cardamine violifolia is a significant Brassicaceae plant known for its high selenium (Se) accumulation capacity, serving as an essential source of Se for both humans and animals. WRKY transcription factors play crucial roles in plant responses to various biotic and abiotic stresses, including cadmium stress, iron deficiency, and Se tolerance. However, the molecular mechanism of CvWRKY in Se accumulation is not completely clear. In this study, 120 WRKYs with conserved domains were identified from C. violifolia and classified into three groups based on phylogenetic relationships, with Group II further subdivided into five subgroups. Gene structure analysis revealed WRKY variations and mutations within the CvWRKYs. Segmental duplication events were identified as the primary driving force behind the expansion of the CvWRKY family, with numerous stress-responsive cis-acting elements found in the promoters of CvWRKYs. Transcriptome analysis of plants treated with exogenous Se and determination of Se levels revealed a strong positive correlation between the expression levels of CvWRKY034 and the Se content. Moreover, CvWRKY021 and CvWRKY099 exhibited high homology with AtWRKY47, a gene involved in regulating Se accumulation in Arabidopsis thaliana. The WRKY domains of CvWRKY021 and AtWRKY47 were highly conserved, and transcriptome data analysis revealed that CvWRKY021 responded to Na2SeO4 induction, showing a positive correlation with the concentration of Na2SeO4 treatment. Under the induction of Na2SeO3, CvWRKY021 and CvWRKY034 were significantly upregulated in the roots but downregulated in the shoots, and the Se content in the roots increased significantly and was mainly concentrated in the roots. CvWRKY021 and CvWRKY034 may be involved in the accumulation of Se in roots. The results of this study elucidate the evolution of CvWRKYs in the C. violifolia genome and provide valuable resources for further understanding the functional characteristics of WRKYs related to Se hyperaccumulation in C. violifolia.
摘要:
Selenium (Se)-rich Cyclocarya paliurus is popular for its bioactive components, and exogenous Se fortification is the most effective means of enrichment. However, the effects of exogenous Se fortification on the nutritional quality of C. paliurus are not well known. To investigate the nutrient contents and antioxidant properties of C. paliurus following Se treatment, we used a foliar spray to apply Se in two forms-chemical nano-Se (Che-SeNPs) and sodium selenite (Na(2)SeO(3)). Sampling began 10 days after spraying and was conducted every 5 days until day 30. The Se, secondary metabolite, malondialdehyde contents, antioxidant enzyme activity, Se speciation, and Se-metabolism-related gene expression patterns were analyzed in the collected samples. Exogenous Se enhancement effectively increased the Se content of leaves, reaching a maximum on days 10 and 15 of sampling, while the contents of flavonoids, triterpenes, and polyphenols increased significantly during the same period. In addition, the application of Se significantly enhanced total antioxidant activity, especially the activity of the antioxidant enzyme peroxidase. Furthermore, a positive correlation between the alleviation of lipid peroxidation and Se content was observed, while methylselenocysteine formation was an effective means of alleviating Se stress. Finally, Na(2)SeO(3) exhibited better absorption and conversion efficiency than Che-SeNPs in C. paliurus.
摘要:
Oxidative stress occurs in the process of egg storage. Antioxidants as feed additives can enhance egg quality and extend the shelf life of eggs. Selenium-enriched Cardamine violifolia (SEC) has strongly antioxidant properties. The objective of this study was to assess the effects of dietary supplementation with SEC on egg quality and the yolk antioxidant capacity of eggs stored at 4 degrees C and 25 degrees C. Four hundred fifty 65-week-old, Roman hens that were similar in laying rate (90.79 +/- 1.69%) and body weight (2.19 +/- 0.23 kg) were divided into 5 groups. The birds were fed diets supplemented with 0 mg/kg selenium (Se) (CON), 0.3 mg/kg Se from sodium selenite (SS), 0.3 mg/kg Se from Se-enriched yeast (SEY), 0.3 mg/kg Se for selenium-enriched Cardamine violifolia (SEC) or 0.3 mg/kg Se from Se-enriched Cardamine violifolia and 0.3 mg/kg Se from Se-enriched yeast (SEC + SEY) for 8 weeks. The eggs were collected on the 8th week and were analyzed for egg quality and oxidative stability of yolk during storage at 4 degrees C or 25 degrees C for 0, 2, 4, or 6 weeks. Dietary SEC and SEC + SEY supplementation increased the Haugh unit (HU) and albumen foam stability in eggs stored at 4 degrees C and 25 degrees C (p < 0.05). SS and SEC supplementation increased the yolk index in eggs stored at 25 degrees C (p < 0.05). SEC or SEC + SEY slowed down an increase in albumen pH and gel firmness in eggs stored at 4 degrees C and 25 degrees C (p < 0.05). Moreover, SEC or SEC + SEY alleviated the increase in malonaldehyde (MDA), and the decrease in total antioxidant capacity (T-AOC) level and total superoxide dismutase (T-SOD) activity in yolks stored at 4 degrees C and 25 degrees C (p < 0.05). These results indicate that SEC mitigated egg quality loss and improved the antioxidant capacity of yolks during storage. SEC supplementation would be advantageous to extend the shelf life of eggs.
摘要:
ATP-binding cassette (ABC) transporters were crucial for various physiological processes like nutrition, development, and environmental interactions. Selenium (Se) is an essential micronutrient for humans, and its role in plants depends on applied dosage. ABC transporters are considered to participate in Se translocation in plants, but detailed studies in soybean are still lacking. We identified 196 ABC genes in soybean transcriptome under Se exposure using next-generation sequencing and single-molecule real-time sequencing technology. These proteins fell into eight subfamilies: 8 GmABCA, 51 GmABCB, 39 GmABCC, 5 GmABCD, 1 GmABCE, 10 GmABCF, 74 GmABCG, and 8 GmABCI, with amino acid length 121-3022 aa, molecular weight 13.50-341.04kDa, and isoelectric point 4.06-9.82. We predicted a total of 15 motifs, some of which were specific to certain subfamilies (especially GmABCB, GmABCC, and GmABCG). We also found predicted alternative splicing in GmABCs: 60 events in selenium nanoparticles (SeNPs)-treated, 37 in sodium selenite (Na(2)SeO(3))-treated samples. The GmABC genes showed differential expression in leaves and roots under different application of Se species and Se levels, most of which are belonged to GmABCB, GmABCC, and GmABCG subfamilies with functions in auxin transport, barrier formation, and detoxification. Protein-protein interaction and weighted gene co-expression network analysis suggested functional gene networks with hub ABC genes, contributing to our understanding of their biological functions. Our results illuminate the contributions of GmABC genes to Se accumulation and tolerance in soybean and provide insight for a better understanding of their roles in soybean as well as in other plants.
期刊:
European Journal of Pharmacology,2024年964:176226 ISSN:0014-2999
通讯作者:
Cheng, SY;Liu, Q
作者机构:
[Li, Xiaoqian; Zhang, Huajie; He, Zhijun; Li, Nan; Liu, Qiong; Shen, Liming] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Biotechnol & Ecol, Shenzhen 518055, Guangdong, Peoples R China.;[Cheng, Shuiyuan; Cheng, SY; He, Zhijun] Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Wuhan 430023, Peoples R China.;[Li, Nan; Liu, Qiong] Shenzhen Hong Kong Inst Brain Sci Shenzhen Fundame, Shenzhen 518055, Peoples R China.;[Liu, Qiong] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen 518055, Peoples R China.
通讯机构:
[Liu, Q ] S;[Cheng, SY ] W;Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Wuhan 430023, Peoples R China.;Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen 518055, Peoples R China.
关键词:
Alzheimer's disease (AD);Cerebral cortex;Esculentoside A (EsA);Proteomics
摘要:
Esculentoside A (EsA), isolated from phytolacca esculenta, is a saponin showing neuroprotective effect in the mouse models of Alzheimer's disease (AD). To investigate its action target and underlying mechanism, this study used the proteomics technique of isobaric tags for relative and absolute quantification (iTRAQ) to analyze the differentially expressed proteins (DEPs) in the cerebral cortex of EsA-treated and untreated triple-transgenic 3×Tg-AD model mice. Proteomic comparison revealed 250, 436, and 903 DEPs in three group pairs, i.e. AD/Wild-type (WT), AD+5mg/kg EsA/AD, AD+10mg/kg EsA/AD, respectively. Among them 28 DEPs were commonly shared by three group pairs, and 25 of them showed reversed expression levels in the diseased group under the treatment of both doses of EsA. Bioinformatics analysis revealed that these DEPs were mainly linked to metabolism, synapses, apoptosis, learning and memory. EsA treatment restored the expression of these proteins, including amyloid precursor protein (APP), cathepsin B (Cstb), 4-aminobutyrate aminotransferase (Abat), 3-phosphoinositide-dependent protein kinase-1 (PDK1), carnitine palmitoyltransferase1 (Cpt1) and synaptotagmin 17 (Syt17), thereby ameliorated the spatial learning and memory of AD mice. Collectively, this study reveals for the first time the profound effect of EsA on the cerebral cortex of AD mice, which might be a potential therapeutic agent for the treatment of AD.
摘要:
Selenium (Se) is a vital micronutrient for human beings, and the global population facing Se deficiency is estimated to be around one billion individuals. To tackle this issue, the enrichment of staple crops with Se has emerged as a potential solution. However, it is important to note that Se can also be detrimental in excessive amounts, and contamination of the environment due to Se from agricultural and industrial sources has resulted in catastrophic ecological disasters over the past half-century. Consequently, the utilization of Se-enriched plants for both human supplementation and phytoremediation purposes has become an invaluable approach towards pollution control. An in-depth comprehension of how plants absorb and metabolize Se is pivotal in the realms of biofortification and phytoremediation. This comprehensive review concisely outlines the origins, mechanisms of absorption, conversion, and metabolism of Se in plants, while also elucidating the various factors that influence its uptake and accumulation. These influential factors encompass soil moisture, organic matter, pH levels, soil texture, microorganisms, and unique plant species characteristics. Furthermore, a thorough analysis of the potential mechanisms that underlie such influences is conducted. It is evident that both biofortification and phytoremediation possess substantial promise in confronting the challenges pertaining to Se, thereby fostering advancements in environmental sustainability. Building upon the current progress in research, this review provides suggestions for future directions aimed at establishing a theoretical framework for Se supplementation in human nutrition and the mitigation of Se-induced pollution.
作者机构:
National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China;School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China;Authors to whom correspondence should be addressed.;[Wenxin Guo; Shuiyuan Cheng; Xinyu Shi; Xin Cong; Lu Wang] National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China;[Hua Cheng; Linling Li] Authors to whom correspondence should be addressed.<&wdkj&>National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China
通讯机构:
[Hua Cheng; Linling Li] A;Authors to whom correspondence should be addressed.<&wdkj&>National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430048, China
摘要:
<jats:p>Selenium nanoparticles (SeNPs) have greater bioavailability and safety than inorganic selenium, and was widely used in medical, agricultural, nutritional supplements, and antibacterial fields. The present study screened a strain L11 producing SeNPs from a selenium rich dairy cow breeding base in Hubei Province, China. The strain was identified as Bacillus subtilis through physiological, biochemical, and molecular biology analysis. By adjusting the cultivation conditions, the experiment determined the ideal parameters for L11 to efficiently produce SeNPs. These parameters include a pH value of 6, a cultivation temperature of 37 °C, a concentration of 4 mmol/L Na2SeO3, and a cultivation of 48 h. X-ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDS), and Transmission Electron Microscopy (TEM) were used to verify that the Se particles produced by L11 are SeNPs with diameters ranging from 50 to 200 nm. The combination of the protein analysis of different cell components and TEM analysis showed that L11 mainly produces SeNPs through the transformation of the cell’s periplasmic space, cell membrane, and cell wall. Adding the L11 SeNPs complex to sheep feed can significantly enhance the antioxidant activity and immunity of sheep, and increase the Se content in the neck muscles, liver, and spleen tissues.</jats:p>
摘要:
Selenium-enriched Cardamine violifolia (SEC), a cruciferous plant, exerts excellent antioxidant and anti-inflammatory capacity, but its effect on hepatic function is unclear. This study investigated the effect and potential mechanism of SEC on hepatic injury induced by lipopolysaccharide (LPS). Twenty-four weaned piglets were randomly allotted to treatment with SEC (0.3 mg/kgSe) and/or LPS (100 μg/kg). After 28 days of the trial, pigs were injected with LPS to induce hepatic injury. These results indicated that SEC supplementation attenuated LPS-induced hepatic morphological injury and reduced aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities in plasma. SEC also inhibited the expression of pro-inflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) after the LPS challenge. In addition, SEC improved hepatic antioxidant capacity via enhancing glutathione peroxidase (GSH-Px) activity and decreasing malondialdehyde (MDA) concentration. Moreover, SEC downregulated the mRNA expression of hepatic myeloid differentiation factor 88 (MyD88) and nucleotide-binding oligomerization domain proteins 1 (NOD1) and its adaptor molecule receptor interacting protein kinase 2 (RIPK2). SEC also alleviated LPS-induced hepatic necroptosis by inhibiting RIPK1, RIPK3, and mixed-lineage kinase domain-like (MLKL) expression. These data suggest that SEC potentially mitigates LPS-induced hepatic injury via inhibiting Toll-like receptor 4 (TLR4)/NOD2 and necroptosis signaling pathways in weaned piglets.
期刊:
International Journal of Biological Macromolecules,2024年280(Pt 2):135586 ISSN:0141-8130
通讯作者:
Cai, Jie
作者机构:
[Cheng, Shuiyuan; Cai, Jie; Liu, Nian; He, Zhijun; Feng, Xiaofang; Xie, Fang] School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China;[Liu, Xiaoqing] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China;[Din, Zia-Ud] Department of Microbiology & Biotechnology, Atta ur Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad 44000, Pakistan;[Luo, Yangchao] Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USA;[Cai, Jie] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China. Electronic address: caijievip@whpu.edu.cn
通讯机构:
[Cai, Jie] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China. Electronic address:
关键词:
OSA starch;Selenium nanoparticles;Stability
摘要:
To develop a promising selenium supplement that overcomes the instability and poor water dispersibility of selenium nanoparticles (SeNPs), we synthesized a series of amphiphilic octenyl succinic anhydride starch (OSAS) through esterification. As the degree of substitution (DS) increased, the particle size of OSAS micelles and the critical micelle concentration (CMC) decreased. FTIR and XRD analysis confirmed the successful introduction of octenyl succinic anhydride groups onto starch. Subsequently, OSAS micelles were used as carriers to synthesize SeNPs via in situ chemical reduction, forming SeNPs-loaded self-assembled starch nano-micelles (OSAS-SeNPs). The OSAS-SeNPs exhibited spherical dispersion in water with an average diameter of 116.1±2.3nm, contributed to enhanced hydrophobic interactions. TEM images showed a core-shell structure with SeNPs as the core and OSAS as the shell. FTIR results indicated hydrogen bonding interactions between OSAS and SeNPs. Due to the negatively charged OSAS shell and hydrogen bonding (OH⋯Se), OSAS-SeNPs remained non-aggregated for one month at room temperature, demonstrating remarkable stability. This study suggests that using OSAS can address the synthesis and stability issues of SeNPs, making it a potential selenium supplement candidate for further evaluation as an anticancer agent.
作者机构:
[Cao, Jie; Xu, Feng; Wang, Li-Na; Zhou, Xian; Zhang, Wei-wei; Ye, Jia-Bao] Yangtze Univ, Coll Hort & Gardening, Jingzhou, Peoples R China.;[Cheng, Shuiyuan; Liu, Xiao-Meng] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;[Xu, Feng; Xu, F] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.
通讯机构:
[Xu, F ] Y;Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.
摘要:
<jats:title>Abstract</jats:title><jats:p>4‐Coumarate‐CoA ligase (<jats:italic>4CL</jats:italic>) gene plays vital roles in plant growth and development, especially the regulation of lignin metabolism and flavonoid synthesis. To investigate the potential function of <jats:italic>4CL</jats:italic> in the lignin biosynthesis of <jats:italic>Ginkgo biloba</jats:italic>, this study identified two <jats:italic>4CL</jats:italic> genes, <jats:italic>Gb4CL1</jats:italic> and <jats:italic>Gb4CL2</jats:italic>, from <jats:italic>G. biloba</jats:italic> genome. Based on the phylogenetic tree analysis, Gb4CL1 and Gb4CL2 protein were classified into Class I, which has been confirmed to be involved in lignin biosynthesis. Therefore, it can be inferred that these two genes may also participate in lignin metabolism. The tissue‐specific expression patterns of these two genes revealed that <jats:italic>Gb4CL1</jats:italic> was highly expressed in microstrobilus, whereas <jats:italic>Gb4CL2</jats:italic> was abundant in immature leaves. The onion transient expression assay indicated that Gb4CL1 was predominantly localized in the nucleus, indicating its potential involvement in nuclear functions, while Gb4CL2 was observed in the cell wall, suggesting its role in cell wall‐related processes. Phytohormone response analysis revealed that the expression of both genes was upregulated in response to indole acetic acid, while methyl jasmonate suppressed it, gibberellin exhibited opposite effects on these genes. Furthermore, <jats:italic>Gb4CL1</jats:italic> and <jats:italic>Gb4CL2</jats:italic> expressed in all tissues containing lignin that showed a positive correlation with lignin content. Thus, these findings suggest that <jats:italic>Gb4CL1</jats:italic> and <jats:italic>Gb4CL2</jats:italic> are likely involved in lignin biosynthesis. Gb4CL1 and Gb4CL2 target proteins were successfully induced in <jats:italic>Escherichia coli</jats:italic> BL21 with molecular weights of 85.5 and 89.2kDa, proving the integrity of target proteins. Our findings provided a basis for revealing that <jats:italic>Gb4CL</jats:italic> participated in lignin synthesis in <jats:italic>G. biloba</jats:italic>.</jats:p>
关键词:
CCoAOMT1 and F3′5′H;differential expression;medicinal active ingredients;molecular mechanism;post-translation level of protein
摘要:
Flavonoids are crucial medicinal active ingredients in Ginkgo biloba L. However, the effect of protein post-translational modifications on flavonoid biosynthesis remains poorly explored. Lysine acetylation, a reversible post-translational modification, plays a crucial role in metabolic regulation. This study aims to investigate the potential role of acetylation in G. biloba flavonoid biosynthesis. Through comprehensive analysis of transcriptomes, metabolomes, proteomes and acetylated proteins in different tissues, a total of 11,788 lysine acetylation sites were identified on 4324 acetylated proteins, including 89 acetylation sites on 23 proteins. Additionally, 128 types of differentially accumulated flavonoids were identified among tissues, and a dataset of differentially expressed genes related to the flavonoid biosynthesis pathway was constructed. Twelve (CHI, C3H1, ANR, DFR, CCoAOMT1, F3H1, F3H2, CCoAOMT2, C3H2, HCT, F3'5'H and FG2) acetylated proteins that might be involved in flavonoid biosynthesis were identified. Specifically, we found that the modification levels of CCoAOMT1 and F3'5'H sites correlated with the catalytic production of homoeriodictyol and dihydromyricetin, respectively. Inhibitors of lysine deacetylase (trichostatin A) impacted total flavonoid content in different tissues and increased flavonoid levels in G. biloba roots. Treatment with trichostatin A revealed that expression levels of GbF3'5'H and GbCCoAOMT1 in stems and leaves aligned with total flavonoid content variations, while in roots, expression levels of GbC3H2 and GbFG2 corresponded to total flavonoid content changes. Collectively, these findings reveal for the first time the important role of acetylation in flavonoid biosynthesis.
期刊:
Journal of Food Engineering,2024年360:111702 ISSN:0260-8774
通讯作者:
Cai, J
作者机构:
[Cheng, Shuiyuan; Zhou, Jiaojiao; Cai, Jie; Fang, Yuan; Liu, Xin; Xie, Fang; Cong, Xin] Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.;[Luo, Yangchao] Univ Connecticut, Dept Nutr Sci, Storrs, CT 06269 USA.;[Din, Zia-ud] Women Univ Swabi, Dept Food Sci & Nutr, Swabi 23430, Khyber Pakhtunk, Pakistan.
通讯机构:
[Cai, J ] W;Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词:
OSA starch;Double emulsions;Plant-derived selenium-enriched peptide;Vitamin E
摘要:
Double emulsions (e.g., O/W/O and W/O/W emulsions) have important applications in healthy food development for food industry. Herein, we prepared O/W/O and W/O/W emulsions by two-step emulsification method only using two food grade emulsifiers (Octenyl succinate starch ester (OSA starch) and polyglycerol polyricinoleate (PGPR)), respectively. The optimal process parameters (in external emulsion and internal emulsion) for the preparation of O/W/O and W/O/W emulsions were systematically studied. It is noteworthy that the facile conversion of the two O/W/O and W/O/W emulsions could be achieved by changing the order of emulsifier addition. The double emulsions can be used for co-loading of plant-derived selenium-enriched peptide (Se peptide) and vitamin E (VE) with efficient loading rates, and the emulsions of encapsulated Se-peptide and VE have good antioxidant properties. The results of fuzzy numerical sensory evaluation and electronic nose showed that both emulsion systems were effective in masking the odor of Se-peptide.
摘要:
Selenium (Se) is an essential trace element for the human body, and its dietary deficiency has been a widespread issue globally. Vegetables serve as a significant source of dietary Se intake, with organic Se derived from plants being safer than inorganic Se. In the present study, Taraxacum mongolicum plants were treated with various concentrations of Na2SeO3. The results showed that as the concentration of Na2SeO3 increased, the chlorophyll content of dandelion seedlings decreased at high concentrations, and the content of soluble sugars, soluble proteins, flavonoids, total phenols, and Vc all increased. The application of Na2SeO3 at concentrations ranging from 0 to 4 mg/L resulted in a reduction in plant malondialdehyde content and an enhancement in the activity of antioxidant enzymes. Following the Na2SeO3 treatment, five Se species were identified in the seedlings, Se4+, Se6+, selenocysteine, selenomethionine, and methylselenocysteine. Notably, selenomethionine emerged as the primary organic Se species in the shoots of dandelion. Transcriptome analysis revealed that ABC11b, PTR4, MOCOS, BAK1, and CNGC1 were involved in the absorption, transport, and storage of Se in dandelion, and C7317 was involved in the scavenging of reactive oxygen species. This study complements the understanding of the possible molecular mechanisms involved in the absorption and transformation of organic Se by plants, thereby providing a theoretical foundation for the biofortification of dandelion with Se in crops.
摘要:
<jats:title>Abstract</jats:title>
<jats:p>Soybean (<jats:italic>Glycine max</jats:italic> L.) sprouts are a common vegetable with rich nutrients, such as protein, vitamin C and isoflavones. Soybean is also capable of accumulating selenium (Se). To study the effects of Se biofortification on the nutrient of this crop, soybean sprouts were treated with different concentrations of selenium nanoparticles (SeNPs) or selenite (i.e., 10 μM, 20 μM, 40 μM, 80 μM and 100 μM) in a hydroponic experiment. Results showed that SeNPs and selenite remarkably increased the total Se concentration in soybean sprouts. Five Se speciations, namely, selenocystine, selenomethionine, methyl selenocysteine, selenite and selenate were detected in soybean sprouts, but selenomethionine was found to be the dominant Se speciation. SeNPs and selenite increased the contents of chlorophyll, carotenoid, soluble sugar, soluble protein, vitamin C and isoflavones in soybean sprouts. SeNPs treatments led to less malondialdehyde content compared with selenite. SeNPs and selenite both enhanced the glutathione content. The modest dosage of exogenous Se stimulated the catalase activity, whereas the large amount reduced it. The peroxidase and ascorbate peroxidase activities were stimulated by SeNPs and selenite. SeNPs posed no significant influence on the superoxide dismutase activity. This study suggests that SeNPs are a good exogenous Se source for the production of Se-rich soybean sprouts.</jats:p>
作者:
Ye, Yuanyuan;He, Jiangling;He, Zhijun;Zhang, Na;Liu, Xiaoqing;...
期刊:
Foods,2023年12(6):1339- ISSN:2304-8158
通讯作者:
Cai, J.;He, J.
作者机构:
[He, Jiangling; Cheng, Shuiyuan; Zhou, Jiaojiao; He, Zhijun; Zhang, Na; Ye, Yuanyuan; Liu, Xiaoqing] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Ye, Yuanyuan; Liu, Xiaoqing] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[He, Zhijun] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Biotechnol & Ecol, Shenzhen 518055, Peoples R China.;[Cai, Jie] Hubei Key Lab Nutr Qual & Safety Agro Prod, Wuhan 430064, Peoples R China.
通讯机构:
[Jie Cai] K;[Jiangling He] A;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, China
作者机构:
[Cheng, Shuiyuan; Rao, Shen; Cong, Xin; Rao, S; Liu, Haodong; Liu, Xiaomeng; Cheng, Hua] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;[Cong, Xin; Gong, Jue] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.
通讯机构:
[Cong, X ; Rao, S] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.
摘要:
Cardamine violifolia, a species belonging to the Brassicaceae family, is a novel vegetable crop that is rich in glucosinolates. However, the specific glucosinolate profiles in this species remain unknown. In the present study, four parts of C. violifolia were collected including central leaves (CLs), outer leaves (OLs), petiole (P), and root (R). The highest level of total glucosinolate was observed in the R. A total of 19 glucosinolates were found in C. violifolia. The predominant glucosinolate compounds were 3-methylbutyl glucosinolate, 6-methylsulfinylhexyl glucosinolate, Indol-3-ylmethyl glucosinolate, 4-methoxyglucobrassicin, and neoglucobrassicin. A transcriptome analysis showed that 16 genes, including BCAT1, BCAT3-6, CYP79A2, CYP79B2-3, CYP83A1, CYP83B1, and SOT17-18, and nine metabolites, such as valine, tryptophan, and 1-methylpropyl glucosinolate, were enriched in the glucosinolate biosynthesis pathway. These genes may be involved in the regulation of glucosinolate accumulation among the four parts. A weighted gene co-expression analysis showed that five genes were predicted to regulate glucosinolate accumulation, including ABC transporter G family member 19, 3-ketoacyl-CoA synthase 19, and pyruvate decarboxylase 1. This study deepens our understanding of the nutrient quality of C. violifolia and provides insights into the regulatory mechanism of glucosinolate accumulation in C. violifolia.