摘要：
Accelerated eutrophication in lakes reduces the number of submerged macrophytes and alters the residues of glyphosate and its degradation products. However, the effects of submerged macrophytes on the fate of glyphosate remain unclear. We investigated eight lakes with varying trophic levels along the middle and lower reaches of the Yangtze River in China, of which five lakes contained either glyphosate or aminomethylphosphate (AMPA). Glyphosate and AMPA residues were significantly positively correlated with the trophic levels of lakes (P<0.01). In lakes, glyphosate is degraded through the AMPA and sarcosine pathways. Eight shared glyphosate-degrading enzymes and genes were observed in different lake sediments, corresponding to 44 degrading microorganisms. Glyphosate concentrations in sediments were significantly higher in lakes with lower abundances of soxA (sarcosine oxidase) and soxB (sarcosine oxidase) (P<0.05). In the presence of submerged macrophytes, oxalic and malonic acids secreted by the roots of submerged macrophytes increased the abundance of glyphosate-degrading microorganisms containing soxA or soxB (P<0.05). These results revealed that a decrease in the number of submerged macrophytes in eutrophic lakes may inhibit glyphosate degradation via the sarcosine pathway, leading to a decrease in glyphosate degradation and an increase in glyphosate residues.
摘要：
In this work, the Mn, Co, Ce co-doped corn cob biochar (MCCBC) as catalytic particle electrodes in a three-dimensional heterogeneous electro-Fenton-like (3D-HEFL) system for the efficient degradation of coking wastewater was investigated. Various characterization methods such as SEM, EDS, XRD, XPS and electrochemical analysis were employed for the prepared materials. The results showed that the MCCBC particle electrodes had excellent electrochemical degradation performances of COD in coking wastewater, and the COD removal and degradation rates of the 3D/HEFL system were 85.35% and 0.0563 min(-1) respectively. RSM optimized conditions revealed higher COD removal rate at 89.23% after 31.6min of electrolysis. The efficient degradability and wide adaptability of the 3D/HEFL system were due to its beneficial coupling mechanism, including the synergistic effect between the system factors (3D and HEFL) as well as the synergistic interactions between the ROS (dominated by •OH and supplemented by O(2)(•-)) in the system. Moreover, the COD removal rate of MCCBC could still remain at 81.41% after 5 cycles with a lower ion leaching and a specific energy consumption of 11.28 kWh kg(-1) COD. The superior performance of MCCBC, as catalytic particle electrodes showed a great potential for engineering applications for the advanced treatment of coking wastewater.
通讯机构：
[Fang, C ] W;Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430023, Peoples R China.
关键词：
Flowering plants;Feature extraction;Image color analysis;Convolutional neural networks;Computational modeling;YOLO;Real-time systems;Image recognition;Agricultural products;Tomato flower fruit recognition;C3Faster;convolutional neural networks;lightweight
摘要：
In order to monitor the growth and development of tomatoes, and improve the efficiency of flower and fruit thinning and tomato picking, this paper constructs a tomato flower and fruit dataset and proposes a TF-YOLOv5s model for the detection of tomato flowers and fruits in natural environments. Based on the YOLOv5s model, a C3Faster module is introduced to reduce the number of parameters and calculations while maintaining detection accuracy. The regular convolution is replaced by depth-wise separable convolution (DWConv) to avoid parameter redundancy. To improve the convergence and accuracy of the model, this paper replaces Complete Intersection over Union (CIoU) loss with Efficient Intersection over Union (EIoU) loss. The Squeeze-and-Excitation (SE) module is added to improve the model’s sensitivity to the features of the tomato flowers and fruits. Compared with the baseline model, the number of parameters is reduced by 54.5%, the weight file is reduced by 52.8%, the Floating-point Operation Per second (FLOPs) is reduced by 48.7%. The detection accuracy of tomato flowers and fruits mAP@0.5 has improved by 1.4% and 1.2% respectively. TF-YOLOv5s is used to detect three types of targets: tomato flowers, red tomatoes, and green tomatoes, and mAP@0.5 of which can reach as high as 95.2%. Furthermore, the improved algorithm is deployed on two edge computing devices to verify its effectiveness. Experimental results show that the algorithm in this paper can achieve high detection with less computational resources. This algorithm has the potential value of application in practical tomato production.
通讯机构：
[Peng, X ] C;[Shen, WJ ] W;Cent China Normal Univ, Inst Environm & Appl Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
关键词：
Cu(II) reduction;Zero-valent iron;Boric acid;Oxygen adsorption;Electron transfer
摘要：
The inhibition of electron transfer by the iron oxide passivation layer and the competition of oxygen for electrons limited the efficiency of heavy metal removal by zero-valent iron reduction. Herein we synthesized micron zero-valent iron modified with boric acid by mechanical ball milling, and demonstrated its enhanced copper ions removal performance. Compared to pristine ZVI, B-ZVI is approximately 2.6 times more efficient in the removal of copper. Interestingly, copper removal was significantly inhibited under air atmosphere for pristine ZVI. However, for B-ZVI, copper removal efficiency was almost insignificantly different between air and argon atmosphere conditions. Furthermore, the modification of ZVI with boric acid promoted the Cu(II) reduction proportion. Boronated modification effectively inhibited oxygen competition for electrons in removing heavy metals by zero-valent iron, which was conducive to the improvement of the heavy metal removal efficiency and the electron utilization of zero-valent iron. This study elucidated the importance of surface modification on the reactivity of micron-sized zero-valent iron and provided an efficient heavy metal removal strategy with zero-valent iron for environmental remediation.
摘要：
Organophosphate flame retardants (OPFRs), a novel class of persistent pollutants, are widely distributed in the environment, and their potential health risks have garnered significant global attention in recent years. Crayfish is a popular freshwater crustacean product in China primarily sourced from the middle and lower reaches of the Yangtze River. The purpose of this study was to investigate the exposure levels of OPFRs in crayfish, assess the health and safety risks associated with crayfish consumption, and explore the bioaccumulation of OPFRs in environmental water and sediment on crayfish. Ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was employed to analyze 7 common OPFRs in 106 crayfish samples and 76 environmental samples. The results revealed that OPFRs were detected at a high frequency of 100% in crayfish, with tripropyl phosphate (TPP) being the predominant pollutant found in edible portions while also exhibiting secondary contamination within the crayfish food chain. Monte Carlo modeling combined with @risk risk assessment software demonstrated that TPP present in crayfish muscles had the most substantial impact on health effects, however, overall OPFR exposure did not pose significant risks to human health. Furthermore, analysis of OPFRs bioenrichment ability indicated that crayfish predominantly accumulated these compounds within their edible parts from surrounding environmental water sources, particularly highlighting TPP's potential for bioaccumulation.
摘要：
In this study, we investigated the protective effect of selenium (Se)-enriched peptide isolated from Cardamine violifolia (SPE) against ethanol-induced liver injury. Cell proliferation assays show that different concentrations of SPE protect human embryonic liver L-02 cells against ethanol-induced injury in a dose-dependent manner. Treatment with 12 μmol/L Se increases the cell survival rate (82.44%) and reduces the release of alanine aminotransferase, aspartate transaminase, lactate dehydrogenase, and apoptosis rate. SPE treatment with 12 μmol/L Se effectively reduces the concentration of intracellular reactive oxygen species and increases the contents of intracellular superoxide dismutase (51.64 U/mg), catalase (4.41 U/mg), glutathione peroxidase (1205.28 nmol/g), and glutathione (66.67 μmol/g), thereby inhibiting the effect of ethanol-induced oxidative damage. The results of the transcriptomic analysis show that the glutathione metabolism and apoptotic pathway play significant roles in the protection of L-02 hepatocytes by SPE. Real-time qPCR analysis shows that SPE increases the mRNA expression of GPX1 and NGFR. The results of this study highlight the protective effects of SPE against ethanol-induced liver injury.
摘要：
This paper presents a stepped electromagnetic bandgap(EBG) hosted on the T-shaped stepped stub with an inverted H-shaped slot etched on the ground to enhance bandwidth and improve isolation for a compact UWB-MIMO antenna developed from our previous design. The characteristic mode analysis is used to offer physical insight into the operation phenomena taking place in the evolution of antenna. The proposed antenna has a compact size of $27\times 22\times 0.8$ mm and realize bandwidth from 3.07 GHz to 11.1 GHz, keeping the isolation more than 20 dB. Key parameters evaluating the antenna performance like radiation pattern, gain, radiation efficiency above 75% and envelope correlation coefficient below 0.05 are investigated. The simulated and measured results agree well, testifying the proposed MIMO antenna is a suitable candidate for UWB applications.
摘要：
Protein homeostasis is essential for cyanobacteria to maintain proper cellular function under adverse and fluctuating conditions. The AAA+ superfamily of proteolytic complexes in cyanobacteria plays a critical role in this process, including ClpXP, which comprises a hexameric ATPase ClpX and a tetradecameric peptidase ClpP. Despite the physiological effects of ClpX on growth and photosynthesis, its potential substrates and underlying mechanisms in cyanobacteria remain unknown. In this study, we employed a streptavidin-biotin affinity pull-down assay coupled with label-free proteome quantitation to analyze the interactome of ClpX in the model cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). We identified 503 proteins as potential ClpX-binding targets, many of which had novel interactions. These ClpX-binding targets were found to be involved in various biological processes, with particular enrichment in metabolic processes and photosynthesis. Using protein-protein docking, GST pull-down, and biolayer interferometry assays, we confirmed the direct association of ClpX with the photosynthetic proteins, ferredoxin-NADP(+) oxidoreductase (FNR) and phycocyanin subunit (CpcA). Subsequent functional investigations revealed that ClpX participates in the maintenance of FNR homeostasis and functionality in Synechocystis grown under different light conditions. Overall, our study provides a comprehensive understanding of the extensive functions regulated by ClpX in cyanobacteria to maintain protein homeostasis and adapt to environmental challenges.
摘要：
American Journal of Alzheimer's Disease & Other Dementias®, Volume 39, Issue , January-December 2024. <br/>ObjectivesTo examine the relationship between hearing impairment and cognitive function and the mediating role of social isolation and depression. Methods: Data came from the 2018 China Health and Retirement Longitudinal Study wave. A self-reported item, a composite index, the 10-item Center for Epidemiological Studies Depression Scale, and the Mini-Mental State Exam were used to measure hearing impairment, social isolation, depression, and cognitive function, respectively. Mediation analysis was performed. Results: 6799 participants were included. For participants reporting mild hearing impairment and severe hearing impairment, there were significant direct and indirect effects on cognitive function. Social isolation mediated 2.75% and 6.33% of the relationship between mild hearing impairment, severe hearing impairment, and cognitive function, respectively. The direct effect of hearing impairment outweighed the mediation effect of social isolation on cognitive function. Conclusions: Decreased cognitive function linked to hearing impairment might benefit from addressing hearing impairment and social isolation in older adults.
关键词：
pea protein isolate;mono- and diglycerides;competition adsorption;quantitative protein proteomics
摘要：
As a commercially available ingredient, the mono- and diglycerides (MDG) were widely used in a plant protein-based emulsion to provide effective, functional, emulsifying properties. The simultaneous addition of the MDG and pea protein isolate (PPI) was investigated by the methods of interfacial rheology and quantitative protein proteomics. The physicochemical quantitative analysis of the oil-water interface revealed an interfacial stability mechanism for the protein adsorption layer. For a low MDG concentration, the interfacial quantities of vicilin and albumin were increased, which could be attributed to the adsorption rate. For a high MDG concentration, both vicilin and albumin were displaced by MDG and desorbed from the interface, while legumin was more difficult to displace due to its slow adsorption and the complex structure of protein molecules. The protein molecules with the structural rearrangement interacted with MDG, exhibiting potential effects on the interfacial film structure. Combined with some nanotechnologies, the new comprehension of protein-emulsifier interactions may promote food delivery systems. The research aims to develop an in-depth analysis of interfacial proteins, and provide more innovative and tailored functionalities for the application of the plant protein emulsion.
摘要：
Latex clearing protein from Streptomyces sp. strain K30 (Lcp(K30)) is a natural oxidoreductase that can catalyse the cleavage of rubber through dioxygenation. It has significant potential applications in polymer degradation. However, its limited expression in engineered strains restricts its utility. This study aimed to enhance the soluble expression and enzyme activity of Lcp(K30) in E. coli BL21 (DE3) by optimizing fermentation conditions and making molecular modifications. The enzyme activity reached 5.05U·mL(-1) by optimizing the induction conditions, adding cofactors, and using chemical chaperones, which was 237.1% of the initial case. Further enhancements in soluble expression were achieved through site mutations guided by the PROSS server, resulting in 8 out of 13 mutants with increased protein expression, a high positive mutation rate of 61.5%. Subsequently, combined mutants were created by merging single mutants with enhanced protein expression and enzyme activity. The top three double mutants, G91D/S149A, G91D/A210H, and G91D/H296P, displayed expression levels at 173.3%, 173.3%, and 153.3% of the wild-type Lcp(K30), respectively. These mutants also exhibited enhanced fermentation enzyme activity, reaching 149.5%, 250.0%, and 420.2% compared to the wild-type, along with improved specific activities. This study provides insights for the efficient production of Lcp(K30) and a practical foundation for its application.
摘要：
This study evaluated changes in the enzyme activity, color, carotenoids, sugar profile, individual phenolic compounds, and antioxidant capacity of supercritical carbon dioxide (SCCD)-treated carrot cubes of different sizes (1 cm and 2 cm). Results demonstrated that SCCD reduced the activities of polyphenol oxidase (PPO) and peroxidase (POD) more in 1 cm carrot cubes than in 2 cm carrot cubes. Nevertheless, the a* , b* , and Delta E values of 1 cm SCCD-treated were enhanced compared to 2 cm SCCD-treated samples. The carotenoids, phenolic compounds, and antioxidant capacity of 1 cm SCCD-treated samples were increased under moderate processing conditions. In contrast, the nutritional composition in 1 cm SCCD-treated samples was lower than in 2 cm SCCDtreated samples at harsh processing conditions. The principal component and correction analysis displayed a visual variation between the enzyme activities and nutritional composition of 1 cm and 2 cm carrot cubes after SCCD processing. According to the results above, 1 cm carrot cubes processed with SCCD at 10 MPa, 35 celcius, and 45 min are the most effective at preserving fresh -cut carrots with relatively high enzyme inactivation and the highest retention of bioactive compounds. This work provides a more comprehensive theoretical basis for optimizing SCCD processing for fresh -cut carrots and recommendations for industrial production.
期刊：
Journal of Materials Science,2024年59(2):715-727 ISSN：0022-2461
通讯作者：
Li, B
作者机构：
[Wu, Aolin; Li, B; Li, Bin; Su, Lihua; Liu, Haiying; Du, Li; Zhou, Mengjing] Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Li, B ] W;Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430023, Hubei, Peoples R China.
摘要：
Preparation of composite hydrogels by in situ polymerization (SP) using choline chloride (ChCl), acrylamide (AM), and urea (U) as raw materials for deep eutectic solvent (DES), with the introduction of PVA and PVDF as fillers into DES. Characterization of the composite hydrogels was conducted using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). This study analyzed the pressure sensitivity, piezoelectricity, swelling behavior, and mechanical properties of the composite hydrogels. The results indicate that due to the dipole–dipole interactions between PVA and PVDF, the composite hydrogels exhibit excellent pressure sensitivity and piezoelectric performance. When the ratio of PVA to PVDF is 1:1, the composite hydrogels exhibit optimal performance, with a 3.37-fold increase in pressure sensitivity and a 6.49-fold increase in piezoelectricity compared to other ratios. This research provides a method for preparing composite hydrogels with excellent piezoelectric performance and pressure sensitivity.
期刊：
Journal of Food Engineering,2024年360:111702 ISSN：0260-8774
通讯作者：
Cai, J
作者机构：
[Cheng, Shuiyuan; Zhou, Jiaojiao; Cai, Jie; Fang, Yuan; Liu, Xin; Xie, Fang; Cong, Xin] Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[Cong, Xin] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.;[Luo, Yangchao] Univ Connecticut, Dept Nutr Sci, Storrs, CT 06269 USA.;[Din, Zia-ud] Women Univ Swabi, Dept Food Sci & Nutr, Swabi 23430, Khyber Pakhtunk, Pakistan.
通讯机构：
[Cai, J ] W;Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
OSA starch;Double emulsions;Plant-derived selenium-enriched peptide;Vitamin E
摘要：
Double emulsions (e.g., O/W/O and W/O/W emulsions) have important applications in healthy food development for food industry. Herein, we prepared O/W/O and W/O/W emulsions by two-step emulsification method only using two food grade emulsifiers (Octenyl succinate starch ester (OSA starch) and polyglycerol polyricinoleate (PGPR)), respectively. The optimal process parameters (in external emulsion and internal emulsion) for the preparation of O/W/O and W/O/W emulsions were systematically studied. It is noteworthy that the facile conversion of the two O/W/O and W/O/W emulsions could be achieved by changing the order of emulsifier addition. The double emulsions can be used for co-loading of plant-derived selenium-enriched peptide (Se peptide) and vitamin E (VE) with efficient loading rates, and the emulsions of encapsulated Se-peptide and VE have good antioxidant properties. The results of fuzzy numerical sensory evaluation and electronic nose showed that both emulsion systems were effective in masking the odor of Se-peptide.
通讯机构：
[Pei, HC ] W;Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430048, Peoples R China.
关键词：
Air-cooled PEMFC stack;Temperature distribution;Cathode air intake mode;Ambient temperature
摘要：
Open-cathode air-cooled proton exchange membrane fuel cells (PEMFCs) simplify conventional cell stacks' cooling and air supply systems, effectively reducing additional energy consumption and improving portability and reliability. As an air fan provides the cathode reaction gas, different air supply conditions significantly influence the output performance of the air-cooled PEMFC stack. This study used a cathode air supply with exhaust and blow modes to investigate the stack performance under different operating conditions. Compared with the exhaust air supply mode, the stack output voltage in the blow-flow mode increased by 5.5 %, and both voltage and temperature uniformities were improved, with 43.0 % and 23.9 % reductions in standard deviation, respectively, when the current density was 300 mA/cm2. In addition, the effect of ambient temperature on stack performance was investigated. Comparing the stack's performance at 15 degrees C and 30 degrees C ambient temperatures, the higher ambient temperature increased the stack's voltage, but led to worsening non-uniform temperature distribution. In the air-cooled PEMFC stack, the influence of the heat generated by the fan on the air-cooled stack temperature could not be ignored. The air supply in the blow-flow mode transfers heat to the stack. At a lower ambient temperature, the temperature increase in the stack was more evident under the exhaust air supply mode.
通讯机构：
[Yang, L ] W;Wuhan Polytech Univ, Coll Mech Engn, Wuhan 430048, Hubei, Peoples R China.
关键词：
Rice starch gel;Crosslink network structure;Rheological property;Direct-write 3D printing
摘要：
Amylopectin and amylose components are natural polymers within rice starch granules, intertwined in specific conditions to form gel polymerized with pore crosslink network, has potential printing properties. In this study, a rice starch gel preparation scheme is proposed for stable properties, and starch granule phase transition mechanism is analyzed based on RVA test during preparation, it can be divided into four-stage, swelling, reacting, homogenizing and self-assembling stages. Gel surface tension and contact angle tested with starch concentration effect, a correlation is developed, reflecting a competition result to gel droplet macro-morphology between the intermolecular cohesion and crosslink network. SEM is used to reveal typical crosslink structures of different starch molecular component proportions, providing objective support for starch gel rheologic property change. Results indicate gel interior crosslink network formed under concentration 12%, the gel with amylose 4.475% presents better printing accuracy. Gel shear modulus positively correlated with amylose proportion. Japonica gel under 20% is of higher viscosity and rapid reassembly ability after interior crosslink network is broken. Max dynamic viscosity is positively correlated with starch concentration. The study aims to provide theoretical and practical support for in-depth analysis of rice starch material application in direct-write 3D printing.
