作者机构：
[Qian, Shenxian; Chen, Zhenzhen; Chen, Can; Shi, Pengfei; Qian, SX] Westlake Univ, Affiliated Hangzhou Peoples Hosp 1, Sch Med, Dept Hematol, Hangzhou, Peoples R China.;[Qian, Shenxian; Chen, Can; Kang, Dandan; Qian, SX] Zhejiang Univ, Sch Med, Hangzhou, Peoples R China.;[Li, Yun; Li, Y] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Team Neonatal & Infant Dev Hlth & Nutr NDHN, Wuhan, Peoples R China.;[Li, Yun; Li, Y] Kindstar Global Precis Med Inst, Wuhan, Peoples R China.
通讯机构：
[Li, Y ; Qian, SX ] W;Westlake Univ, Affiliated Hangzhou Peoples Hosp 1, Sch Med, Dept Hematol, Hangzhou, Peoples R China.;Zhejiang Univ, Sch Med, Hangzhou, Peoples R China.;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Team Neonatal & Infant Dev Hlth & Nutr NDHN, Wuhan, Peoples R China.;Kindstar Global Precis Med Inst, Wuhan, Peoples R China.
关键词：
Biomarker;CD8 + T cells;DLBCL;DLD;KDM1A
摘要：
Since the discovery of copper induces cell death(cuprotosis) in 2022, it has been one of the biggest research hotspots. cuprotosis related genes (CRGs) has been demonstrated to be a potential therapeutic target for cancer, however, the molecular mechanism of CRGs in coronavirus disease 2019 (COVID-19) infected in DLBCL patients has not been reported yet. Therefore, our research objective is first to elucidate the mechanism and role of CRGs in COVID-19. Secondly, we conducted univariate and multivariate analysis and machine learning to screen for CRGs with common expression differences in COVID-19 and DLBCL. Finally, the functional role and immune mechanism of genes in DLBCL were confirmed through cell experiments and immune analysis. The research results show that CRGs play an important role in the occurrence and development of COVID-19. Univariate analysis and machine learning confirm that dihydrolipoamide dehydrogenase (DLD) is the common key gene of COVID-19 and DLBCL. Inhibiting the expression of DLD can significantly inhibit the cycle progression and promote cell apoptosis of DLBCL cells and can target positive regulation of Lysine-specific demethylase 1 (LSD1, also known as KDM1A) to inhibit the proliferation of DLBCL cells and promote cell apoptosis. The immune analysis results show that high-expression of DLD may reduce T cell-mediated anti-tumor immunity by regulating immune infiltration of CD8 + T cells and positively regulating immune checkpoints LAG3 and CD276. Reducing the expression of DLD can effectively enhance T cell-mediated anti-tumor immunity, thereby clearing cancer cells and preventing cancer growth. In conclusion, DLD may be a potential therapeutic target for COVID-19 infection in DLBCL patients. Our research provides a theoretical basis for improving the clinical treatment of COVID-19 infection in DLBCL.
摘要：
Selenium-enriched Cardamine violifolia (SEC), a cruciferous plant, exerts excellent antioxidant and anti-inflammatory capacity, but its effect on hepatic function is unclear. This study investigated the effect and potential mechanism of SEC on hepatic injury induced by lipopolysaccharide (LPS). Twenty-four weaned piglets were randomly allotted to treatment with SEC (0.3 mg/kgSe) and/or LPS (100 μg/kg). After 28 days of the trial, pigs were injected with LPS to induce hepatic injury. These results indicated that SEC supplementation attenuated LPS-induced hepatic morphological injury and reduced aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities in plasma. SEC also inhibited the expression of pro-inflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) after the LPS challenge. In addition, SEC improved hepatic antioxidant capacity via enhancing glutathione peroxidase (GSH-Px) activity and decreasing malondialdehyde (MDA) concentration. Moreover, SEC downregulated the mRNA expression of hepatic myeloid differentiation factor 88 (MyD88) and nucleotide-binding oligomerization domain proteins 1 (NOD1) and its adaptor molecule receptor interacting protein kinase 2 (RIPK2). SEC also alleviated LPS-induced hepatic necroptosis by inhibiting RIPK1, RIPK3, and mixed-lineage kinase domain-like (MLKL) expression. These data suggest that SEC potentially mitigates LPS-induced hepatic injury via inhibiting Toll-like receptor 4 (TLR4)/NOD2 and necroptosis signaling pathways in weaned piglets.
期刊：
Construction and Building Materials,2024年411:134273 ISSN：0950-0618
通讯作者：
Zheng, ZS;Huang, YJ
作者机构：
[Zeng, Chen] Wuhan Polytech Univ, Sch Civil Engn & Architecture, Wuhan 430023, Peoples R China.;[Liu, Gang; Wang, Xiang; Zheng, ZS; Zheng, Zhi-shan] Wuhan Municipal Engn Design & Res Inst Co Ltd, Wuhan 430023, Peoples R China.;[Zhang, Hui; Huang, Yu-jie] North Univ China, Sch Environm & Safety Engn, Taiyuan 030051, Peoples R China.
通讯机构：
[Zheng, ZS ] W;[Huang, YJ ] N;Wuhan Municipal Engn Design & Res Inst Co Ltd, Wuhan 430023, Peoples R China.;North Univ China, Sch Environm & Safety Engn, Taiyuan 030051, Peoples R China.
关键词：
Non-uniform corrosion of steel;3D mesoscale model;Mass transfer;Electrochemical corrosion;Bi-directional erosion
摘要：
Chloride-induced corrosion of steel in reinforced concrete (RC) exhibits complicated mechanisms such as chlo-ride diffusion, oxygen diffusion, electrochemical polarization, dissolution and deposition of steel. This work investigates the 3D non-uniform corrosion behavior of steel in RC by integrating all the possible mechanisms and mesoscale heterogeneities, for the first time. The anodic Tafel slope is used to describe the depassivation of steel induced by chloride, while the dynamic process of oxygen supply and consumption is considered as well as the inhibition of the cathodic reaction caused by oxygen concentration. The coupling of mass transfer and electro-chemical corrosion in RC is developed and validated. Mesoscale RC models with realistic aggregate, mortar, steel and interface are generated through a physically-based approach similar to casting procedures. The simulation results show that the 3D mesoscale RC model can simultaneously capture the non-uniform corrosion patterns of steel along the circumferential and longitudinal directions, offering significant application value in accurately predicting steel corrosion morphology. The random distribution of aggregates affects the local non-uniform corrosion of steel, but has little influence on the overall cumulative corrosion level. The local non-uniform corrosion of steel gradually weakens with the increase in chloride erosion time. The non-uniform corrosion of side steel is enhanced with the increase of the steel diameter and the decrease of the protective layer thickness. Bi-directional erosion mainly enhances the uniform corrosion of corner steel.
摘要：
Four typical forest types in Sygera Mountain, namely, Pinus armandii (PA), Picea likiangensis var. linzhiensis (PLL), Abies georgei var. smithii (AGS), and Juniperus saltuaria (JS) were considered using methods such as analysis of variance and stoichiometry. Effects of changes of nutrient content and stoichiometric ratio of various organs of arbor plants were evaluated. Compared with global plant nutrients, this study area had lower N and P contents. The growth of the Juniperus saltuaria forest was jointly restricted by N and P, and as the environmental gradient decreased, the growth of the other three forest types changed from N and P joint restrictions to N restrictions. The distribution order of element content in various organs among different forest types was consistent as follows: leaf>branch>root>trunk. There was a strong domestication reaction between N and P contents. This led to a significantly higher C/N ratio of the square branch Berlin compared to the other three forest types, while the C/P and N/P ratios were opposite. This may be due to the "optimal allocation principle" of nutrients in various organs under different environmental conditions. The nutrient allocation patterns of plant organs in different forest types were not the same, which reflects the differences in life strategies and nutrient utilization among different forest species.
摘要：
Due to their large surface area and pore volume, three-dimensional covalent organic frameworks (3D COFs) have emerged as competitive porous materials. However, structural dynamic behavior, often observed in imine-linked 3D COFs, could potentially unlock their potential application in gas storage. Herein, we showed how a pre-locked linker strategy introduces breaking dynamic behavior in 3D COFs. A predesigned planar linker-based 3,8-diamino-6-phenylphenanthridine (DPP) was prepared to produce non-dynamic 3D JUC-595, as the benzylideneamine moiety in DPP locked the linker flexibility and restricted the molecular bond rotation of the imine linkages. Upon solvent inclusion and release, the PXRD profile of JUC-595 remained intake, while JUC-594 with a flexible benzidine linker experienced crystal transformation due to framework contraction-expansion. As a result, the activated JUC-595 achieved higher surface areas (754 m2 g-1) than that of JUC-594 (548 m2 g-1). Furthermore, improved CO2 and CH4 storages were also seen in JUC-595 compared with JUC-594. Impressively, JUC-595 recorded a high normalized H2 storage capacity that surpassed other reported high-surface area 3D COFs. This works shows important insights on manipulating the structural properties of 3D COF to tune gas storage performance.
摘要：
The production and sales of electric vehicle lithium-ion batteries (EV LIB) have experienced rapid growth in the past decade, resulting in an extensive and intricate supply chain network (SCN). The SCNs orchestrated by different focal firms are interdependent due to the joint suppliers and customers. This interdependence, coupled with geographical segmentation, introduces vulnerability to the global EV LIB SCN. Focal firms need management insights from the multiplexity of the global EV LIB SCN to address the potential vulnerability. This investigation utilizes the empirical data regarding the supplier-buyer relationships of the top six EV LIB manufacturers in 2020 to construct the multilayered EV LIB SCN. Then, a focal firm-specific analysis is conducted to explore the multiplexity of the global EV LIB SCN, including identifying the influential firms, measuring the structure of SCNs led by different focal firms, and distinguishing the dependence and discrepancies of different focal firms' SCNs. Furthermore, an SCN vulnerability (SCNV) index was designed to assess the vulnerability of such a multilayered SCN in two disruption scenarios, i.e., single-country blockade and intercountry blockade. The structural analysis can assist focal firms in allocating vulnerability-coping resources more effectively. Besides, the assessment of SCNV can assist managers in identifying potential risk sources beyond their own SCNs.
期刊：
Journal of Cleaner Production,2024年435:140388 ISSN：0959-6526
通讯作者：
Zhou, J;Zhang, WJ;Ning, XP
作者机构：
[Zhou, Ju; Zhou, J] Luoyang Normal Univ, Business Sch, Luoyang 471934, Peoples R China.;[Xu, Ning] Henan Normal Univ, Sch Polit Sci & Publ Adm, Xinxiang 453007, Peoples R China.;[Zhang, WJ; Zhang, Wenjie] Wuhan Polytech Univ, Sch Management, Wuhan 430048, Peoples R China.;[Ning, XP; Ning, Xueping] Shanghai Univ Int Business & Econ, Sch Int Business, Shanghai 201620, Peoples R China.
通讯机构：
[Zhang, WJ ] W;[Ning, XP ] S;[Zhou, J ] L;Luoyang Normal Univ, Business Sch, Luoyang 471934, Peoples R China.;Wuhan Polytech Univ, Sch Management, Wuhan 430048, Peoples R China.
关键词：
New-type urbanization pilot policy;Agricultural low-carbon development;Agricultural technological efficiency;Difference-in-differences model
摘要：
Advancing agricultural low -carbon development (ALCD) holds substantial real -world significance for the lowcarbon sustainable growth of the global economy. Traditional urbanization, focusing on the "one-way" concentration of elements in cities and pursuing urban expansion's scale and speed, poses a threat to ALCD. In contrast, new -type urbanization (NTU), which emphasizes a "two-way" concentration of elements in both rural and urban areas, provides an approach to address this issue. Nevertheless, no research has investigated the relationship between NTU, particularly the new -type urbanization pilot policy (NTUPP), and ALCD. To fill this void, this research employs panel data from 210 Chinese cities from 2006 to 2019, using a difference -indifferences (DID) model to investigate the impact and mechanisms of NTUPP on ALCD for the first time. The study reveals: (1) NTUPP significantly boosts the level of ALCD, with cities that have implemented NTUPP showing an average enhancement of 19.13% in ALCD levels compared to those without NTUPP. (2) NTUPP chiefly fosters ALCD by improving agricultural technological efficiency rather than advancing technological progress. (3) Relative to smaller cities, northern cities, eastern cities, resource -based cities, major grainproducing areas, cities with substantial government intervention, and cities with lower levels of financial development, NTUPP is more efficacious in enhancing ALCD in larger cities, southern cities, central and western cities, non -resource -based cities, non -major grain -producing areas, cities with minimal government intervention, and cities with advanced financial development. (4) NTUPP advances ALCD in local cities and boosts ALCD in neighboring cities via spatial spillover effects. This research aids the Chinese government in clarifying the implementation effects of the new -type urbanization pilot policy based on its findings, offering theoretical underpinnings and practical evidence for government departments in shaping and assessing the policy, and providing insights and evidence for other nations worldwide to advance sustainable agricultural development via enhanced urbanization efforts.
摘要：
Our previous study revealed that green tea polysaccharide conjugate (gTPC) has emulsion effect, but its emulsifying ability is weak. In order to improve the emulsification ability of gTPC, gTPC and bovine serum albumin (BSA) were combined to form five different mass proportions of the TPC/BSA (TB) complex: TPC/BSA: 5:1, 5:2, 5:3, 5:4, and 5:5 w/w. We observed that the 5:5 w/w TB emulsion was more hydrophobic and surface-active. Furthermore, the emulsions prepared using 50.00wt% medium-chain triglycerides exhibited the best stability. In addition, the TB emulsion exhibited stability in adverse environments of pH, salt, and heat; in particular, under salt conditions, no significant changes were observed in zeta potential. Subsequently, in vitro simulated digestion experiments were performed to investigate the use of TB emulsions for β-carotene encapsulation. We observed that the encapsulation efficiency for β-carotene was approximately 90.0%; it was subsequently released in the intestine.
摘要：
Two-dimensional transition metal disulfides (TMDs) heterojunction is a very promising non-precious metal catalyst that has been widely used as a catalyst for hydrogen evolution reactions. In this paper, the effects of different positions of Se vacancies on the electronic structure and hydrogen evolution reaction electrocatalyst of MoSe2/WSe2 heterojunction are investigated by first-principles calculations. After the introduction of vacancy, defect states appear between the conduction band bottom and the Fermi energy level of the heterojunction. This is more favorable for the electron transfer between H and the substrate. The results of the density of states calculations indicate that the defect states appear because the d orbitals of the transition metal atoms containing the Se vacancy layer hybridize with the p orbitals of the Se atoms. The differential charge density indicates that the Se vacancies promote the charge transfer of MoSe2/WSe2. In addition, calculations of the Gibbs free energy for the hydrogen evolution reaction of heterojunctions show that Se vacancies (especially the outer vacancies) enhance the electrocatalytic hydrogen precipitation performance of heterojunctions. The results provide a new way to regulate the hydrogen evolution properties of MoSe2/WSe2 heterojunctions.
作者机构：
[Cao, Jie; Xu, Feng; Wang, Li-Na; Zhou, Xian; Zhang, Wei-wei; Ye, Jia-Bao] Yangtze Univ, Coll Hort & Gardening, Jingzhou, Peoples R China.;[Cheng, Shuiyuan; Liu, Xiao-Meng] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;[Xu, Feng; Xu, F] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.
通讯机构：
[Xu, F ] Y;Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.
摘要：
4-Coumarate-CoA ligase (4CL) gene plays vital roles in plant growth and development, especially the regulation of lignin metabolism and flavonoid synthesis. To investigate the potential function of 4CL in the lignin biosynthesis of Ginkgo biloba, this study identified two 4CL genes, Gb4CL1 and Gb4CL2, from G. biloba genome. Based on the phylogenetic tree analysis, Gb4CL1 and Gb4CL2 protein were classified into Class I, which has been confirmed to be involved in lignin biosynthesis. Therefore, it can be inferred that these two genes may also participate in lignin metabolism. The tissue-specific expression patterns of these two genes revealed that Gb4CL1 was highly expressed in microstrobilus, whereas Gb4CL2 was abundant in immature leaves. The onion transient expression assay indicated that Gb4CL1 was predominantly localized in the nucleus, indicating its potential involvement in nuclear functions, while Gb4CL2 was observed in the cell wall, suggesting its role in cell wall-related processes. Phytohormone response analysis revealed that the expression of both genes was upregulated in response to indole acetic acid, while methyl jasmonate suppressed it, gibberellin exhibited opposite effects on these genes. Furthermore, Gb4CL1 and Gb4CL2 expressed in all tissues containing lignin that showed a positive correlation with lignin content. Thus, these findings suggest that Gb4CL1 and Gb4CL2 are likely involved in lignin biosynthesis. Gb4CL1 and Gb4CL2 target proteins were successfully induced in Escherichia coli BL21 with molecular weights of 85.5 and 89.2kDa, proving the integrity of target proteins. Our findings provided a basis for revealing that Gb4CL participated in lignin synthesis in G. biloba.
通讯机构：
[Chen, RS ; Ren, XH ] W;Wuhan Univ Sci & Technol, Inst Adv Mat & Nanotechnol, Sch Chem & Chem Engn, State Key Lab Refractories & Met, Wuhan 430081, Peoples R China.;Wuhan Univ Sci & Technol, Minist Educ, State Key Lab Refractories & Met & Resources Utili, Key Lab Ferous Metalurgy, Wuhan 430081, Peoples R China.;Wuhan Univ Sci & Technol, Fac Mat, Hubei Prov Key Lab New Proc Ironmaking & Steel mak, Wuhan 430081, Peoples R China.
关键词：
CO2RR;Copper-based catalyst;Pyridine;Modification;Surface active sites
摘要：
Copper -based catalysts have been extensively investigated for electrocatalytic reduction of CO2 owing to their unique capabilities. To our notice, organic molecules can be employed as molecular modifiers to tailor the CO2 reduction selectivity of Cu -based catalysts. Herein, three kinds of molecular modifiers, pyridine, triethylamine, and potassium hydroxide have been utilized for fabricating Cu -based electrocatalysts. Experimental investigations reveal that pyridine as molecular modifiers have the ability to alter the surface active sites of the Cubased catalysts. The modification can facilitate the conversion of CO2 into HCOOH. Notably, the highest Faradaic efficiency achieved in our tests for HCOOH can reach to an impressive value of 80.2 %. Moreover, the catalyst maintains its structure and performance with high stability that exceeding 10 h at a current density of 30 mA cm -2 (-1.1 V vs. RHE). Based on structural characterization and theoretical calculations, we propose that the pyridine molecule can facilitate CO2 accumulation, and the pyridine modification of the CuO/Cu(OH)2 surface active site can reduce the reaction energy barrier for the electrocatalytic reduction of CO2 to HCOOH.
摘要：
Quicklime (CaO) or reactive magnesia (MgO) could be utilized as a novel activator of ground granulated blast-furnace slag (GGBS) to produce the geopolymer. The geopolymer was used to solidify soft soil, showing a significant environmental benefit over conventional Portland cement. In this study, the geopolymers were made according to the weight ratio of CaO/MgO to GGBS of 1:9 and 2:8 and were further used to solidify silty clay. The engineering and microstructural characteristics of the solidified silty clay were investigated through various physicochemical, mechanical, and microscopic tests. The results indicated that the unconfined compressive strength of the solidified specimens increased with the increase of activator ratio and curing period. The CaO-GGBS (CG)-solidified soil had a higher unconfined compressive strength than the MgO-GGBS (MG)-solidified soil after 7-day curing, while the MG-solidified soil showed good mechanical properties in the long run. The pH and electrical conductivity of the solidified soils gradually decreased with the decreasing binder dosage and the increasing curing period, and these values of MG-solidified soils were lower than those of CG-solidified soils. Based on XRD analysis, the CSH and hydrotalcite were confirmed to be the main hydration product of both CG/MG-solidified soils in filling the large pores, and the reinforcement mechanism model for the soil treated with CG/MG geopolymers was also proposed. The research results demonstrated that CG/MG geopolymers can be used as a binder to solidify soft soils.
摘要：
To investigate the molecular etiology of low sperm quality in patients with intractable spermatocystitis, spermatozoa samples from patients with persistent hematospermia undergoing transurethral seminal vesiculoscopy and healthy volunteers were utilized. Spermatozoa samples were collected from the seminal vesicles through transurethral seminal vesiculoscopy or by masturbation ejaculation. Sperm quality was analyzed by a WLJY-9000 color semen analysis system. Measurement of tumor necrosis factor alpha (TNFα) and interleukin-6 (IL-6) in the seminal plasma was performed using enzyme-linked immunosorbent assay (ELISA). Measurement of H2O2 in the seminal plasma was performed with a hydrogen peroxide kit. The protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and phosphorylated-Nrf2 (p-Nrf2) were measured by western blot analysis and immunofluorescence assays. Low sperm quality parameters and increased levels of inflammatory cytokines (TNFα, IL-6, and H2O2) in the seminal plasma were detected among the semen samples from the patients with persistent hematospermia. Nrf2 and p-Nrf2 were strongly expressed in the nucleus and periphery of human sperm cells, according to the results of the immunofluorescence assays. The protein levels of Nrf2 and p-Nrf2 were significantly lower in the spermatozoa samples from patients with persistent hematospermia than in those from healthy volunteers with normal sperm motility. The results suggested that Nrf2 signaling might play a role in the low sperm quality of patients with intractable spermatocystitis.
关键词：
train operation;vibration;construction stage;transfer function;finite element model
摘要：
In response to land use challenges, major urban centers have started implementing over-track building constructions above metro lines as a means of accommodating residents and workers. However, the continuous operation of trains can generate excessive vibrations that may negatively impact the overall living conditions for occupants residing in these structures. In this paper, vibration measurements were conducted on the soil and within a three-story frame structure building. Additionally, a three-dimensional finite element model of the track-soil-building was established. The wheel-rail contact force was incorporated as a dynamic load that varies with time to accurately simulate the vibration response induced by trains. According to the construction process of the over-track building, four construction stages were set up using the finite element model to study the impact of the construction stages on the vibration propagation from the soil to building structure. The results indicate that the presence of existing structures exerts a mitigating influence on soil vibrations. Pile foundation construction can effectively mitigate soil vibration to a significant extent. The findings provide references for the future development and design of over-track buildings.
摘要：
To investigate the efficacy of four forms of methionine (Met) supplementation in red swamp crayfish (Procambarus clarkii), a Met-deficient all-plant protein diet was used control, along with four supplemental diets: 0.90 g/kg Met-Met, 1.80 g/kg crystal DL-Met, 3.60 g/kg E-Met, and 3.29 g/kg MHA-Ca. The analysis Met + Cys levels of five diets were 10.46 (control), 11.51, 12.50, 12.56, and 10.70 g/kg, respectively. Each diet was hand-fed to five replicates of 18 crayfish each (initial body weight: 4.40 +/- 0.02 g) for eight weeks. Supplementation with four forms of exogenous Met resulted in an increase in weight gain rate, specific growth rate, and antioxidant capacity, while decreasing the feed conversion ratio (P < 0.05). The abdominal muscle crude protein content and the protein deposition rate in the Met-Met and E-Met groups were higher than those of the control group (P < 0.05). The deposition rate of Met, arginine, leucine, lysine, threonine, and valine, and the activities of digestive enzymes were higher in the Met-Met group than that of the control group (P < 0.05). The peak times and values of serum biochemical indices were affected by four forms of exogenous Met. Additionally, the hepatopancreatic homocysteine content in the MHA-Ca group was higher than in the control group (P < 0.05). In conclusion, the utilization of Met-Met by Procambarus clarkii was superior to other forms of Met in an all-plant protein diet.
摘要：
We explored the resource acquisition and growth strategies of plants adapting to different environments, focusing on the typical forest types of Sygera Mountain: Pinus armandii, Picea likiangensis var. Linzhiensis, Abies georgei var. Smithii, and Juniperus saltuaria. Then, we analyzed the nutrient content and stoichiometric ratios of C, N, P, and K in different plant organs (leaves, branches, trunks, and roots) to examine the stoichiometric characteristics and nutrient balance mechanisms in these forests. Results show that within the same forest type, different plant organs exhibit high C and low N, P, and K levels. N content in all organs followed the order leaves > branches > roots > trunks. During the growth phase, the concentrations of P and K in PLL and AGS follow the order branches > leaves > roots > trunks. In the dormant phase, the distribution in different organs had the order leaves > branches > roots > trunks. C content remained relatively stable over time. In the same organ across different forest types, increase in nitrogen content in plant leaves is an active adaptation of JS plants, indicating that JS has a conservative growth strategy and can adapt to environmental stress. Owing to the influence of seasons, the evolution process of N and P content fluctuates, allocating nutrients to supporting and transporting organs for resource optimization and allocation. The N and P content were lower in the growth phase than in the dormant phase. Seasonal variations in the C/N, C/P, and C/K ratios in different forests were inversely correlated with changes in N, P, and K content in plant organs, supporting the "growth rate hypothesis." Stoichiometric analysis suggests that different limiting elements exist in organs across various forest types. Principal component analysis indicates that the seasonal patterns of stoichiometric ratios in the organs of different forest types show species-specific characteristics, reflecting the evolutionary nutrient utilization strategies of plant genera. In summary, plant growth in different Sygera Mountain forest types is limited by N and P, with a high tendency toward nitrogen limitation. The nutrient utilization and distribution differences among various organs during different growth stages are primarily influenced by the limited availability of environmental nutrients and inherent physiological characteristics of the plants.
摘要：
Nicotinamide mononucleotide (NMN) could reduce seizure intensity and improve cognitive function in epileptic animals. Additionally, NMN exhibited inhibitory effects on neuronal apoptosis. These therapeutic effects were found associated with the down‐regulation of fission proteins (Drp1 and Fis1) and the up‐regulation of fusion proteins (Mfn1 and Mfn2) through the SIRT1‐PGC‐1α pathway, thus promoting mitochondrial energy metabolism protection. These findings suggest that NMN intervention may serve as a novel potential therapeutic approach for epilepsy seizure (SE)‐induced cognitive impairment and behavioral disorders. Targeting the SIRT1‐PGC‐1α pathway represents a promising therapeutic strategy for addressing SE. Abstract Both human and animal experiments have demonstrated that energy metabolism dysfunction in neurons after seizures is associated with an imbalance in mitochondrial fusion/fission dynamics. Effective neuronal mitochondrial dynamics regulation strategies remain elusive. Nicotinamide mononucleotide (NMN) can ameliorate mitochondrial functional and oxidative stress in age‐related diseases. But whether NMN improves mitochondrial energy metabolism to exert anti‐epileptic effects is unclear. This study aims to clarify if NMN can protect neurons from pentylenetetrazole (PTZ) or Mg2+‐free‐induced mitochondrial disorder and apoptosis via animal and cell models. We established a continuous 30‐day PTZ (37 mg/kg) intraperitoneal injection‐induced epileptic mouse model and a cell model induced by Mg2+‐free solution incubation to explore the neuroprotective effects of NMN. We found that NMN treatment significantly reduced the seizure intensity of PTZ‐induced epileptic mice, improved their learning and memory ability, and enhanced their motor activity and exploration desire. At the same time, in vitro and in vivo experiments showed that NMN can inhibit neuronal apoptosis and improve the mitochondrial energy metabolism function of neurons. In addition, NMN down‐regulated the expression of mitochondrial fission proteins (Drp1 and Fis1) and promoted the expression of mitochondrial fusion proteins (Mfn1 and Mfn2) by activating the SIRT1‐PGC‐1α pathway, thereby inhibiting PTZ or Mg2+‐free extracellular solution‐induced mitochondrial dysfunction, cell apoptosis, and oxidative stress. However, combined intervention of SIRT1 inhibitor, Selisistat, and PGC‐1α inhibitor, SR‐18292, eliminated the regulatory effect of NMN pre‐treatment on mitochondrial fusion and fission proteins and apoptosis‐related proteins. Therefore, NMN intervention may be a new potential treatment for cognitive impairment and behavioral disorders induced by epilepsy, and targeting the SIRT1‐PGC‐1α pathway may be a promising therapeutic strategy for seizures.
摘要：
As an advanced time-frequency (TF) decomposition (TFD) method, variational nonlinear chirplet mode decomposition (VNCMD) decomposes the original signal into a series of nonlinear chirplet modes (NCMs), such that the inherent characteristic information contained in the signal can be revealed effectively. However, the decomposition ability of VNCMD is largely affected by the prior instantaneous frequency (IF) and the pre-set parameters. In practical engineering applications, the presence of noise and interference components often complicates the accurate determination of prior IFs and appropriate decomposition parameters. Considering the above issues, in order to precisely extract the NCMs and realize the effective analysis of mechanical vibration signals, this paper mainly focuses on the drawbacks of accurate prior IF and the decomposition parameters of VNCMD, and proposed an improved version via local maximum synchrosqueezing transform and a recursive mode extracting scheme. The performance of the proposed method is evaluated through simulation cases, and the results demonstrate its effectiveness. Finally, the proposed method is successfully applied to bearing data analysis and rub-impact fault detection.
通讯机构：
[Chang, C; Wu, JE ] W;Wuhan Polytech Univ, Coll Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.
关键词：
Adding sequence;Curcumin;Heating pH;Self-aggregated;Zein/whey protein isolate (WPI)/pectin nanoparticles
摘要：
In this work, pectin was employed as a coating material to fabricate zein/whey protein isolate (WPI)/pectin complex nanoparticles via a pH-adjusted and heat-induced electrostatic adsorption process for potential oral administration applications of curcumin. Factors such as the order of raw material addition, heating temperature and pH, and zein concentration were comprehensively examined. In addition to electrostatic interactions, Fourier transform infrared and fluorescence spectroscopy indicated that hydrophobic interactions and hydrogen bonds were also involved in the development of complex nanoparticles. The complex nanoparticles obtained not only improved the antioxidant activity of curcumin in aqueous phase, but also contributed to its controlled release under gastrointestinal conditions. Our findings revealed that the heating pH and adding sequence of raw materials had a notable impact on the properties of complex nanoparticles, and that pectin coating had an exceptional stabilizing effect on complex nanoparticles under gastrointestinal circumstances. This study provides novel insights and perspectives for the preparation of polysaccharide-protein complex nanoparticles, signifying the potential use of zein/WPI/pectin complex nanoparticles as delivery vehicles in the functional food and pharmaceutical industries.
摘要：
In the present study, we demonstrated that the presence of cysteine could remarkably enhance the degradation of atrazine by Fe(3)O(4)/persulfate system. The results of electron paramagnetic resonance (EPR) spectra confirmed the combination of cysteine and Fe(3)O(4) exhibited much higher activity on activation of persulfate to generate more SO(4)(•-) and •OH than Fe(3)O(4) alone. At pH of 3.0, SO(4)(•-) and •OH contributed to about 58.2% and 41.8% of atrazine removal respectively, while •OH gradually dominated the oxidation of atrazine from neutral condition to alkaline condition. The co-existing Cl(-) and HCO(3)(-) could quench SO(4)(•-), resulting in the inhibition of atrazine degradation. The presence of low natural organic matters (NOM) concentration (0-2mgL(-1)) could enhance the atrazine removal, and high concentration (>5mgL(-1)) of NOM restrained the atrazine degradation. During the Cysteine/Fe(3)O(4)/Persulfate process, cysteine served as a complexing reagent and reductant. Through acidolysis and complexation, Fe(3)O(4) could release dissolved and surface bound Fe(2+), both of which contributed to the activation of persulfate together. Meanwhile, cysteine was not rapidly consumed due to a regeneration process, which was beneficial for maintaining Fe(2+)/Fe(3+) cycle and constantly accelerating the activation of persulfate for atrazine degradation. The reused Fe(3)O(4) and cysteine in the Cysteine/Fe(3)O(4)/Persulfate process exhibited high stability for the atrazine degradation after three cycles. The degradation pathway of atrazine included alkylic-oxidation, dealkylation, dechlorination-hydroxylation processes. The present study indicates the novel Cysteine/Fe(3)O(4)/Persulfate process might be a high potential for treatment of organic polluted water.
