荆楚卓越水产人才协同培养长效机制的构建与思考
作者：
刘立鹤;刘军;黄峰;余登航
期刊：
武汉轻工大学学报 ,2019年38(2):115-121 ISSN：2095-7386
作者机构：
武汉轻工大学动物科学与营养工程学院,湖北武汉,430023;[刘军; 余登航; 黄峰; 刘立鹤] 武汉轻工大学
关键词：
荆楚卓越人才计划;长效机制;协同培养型;水产专业人才培养
摘要：
在水产养殖产品升级与经营转型的大背景下,借我校水产养殖专业列入湖北省“荆楚卓越人才”协同育人计划的契机,针对水产养殖专业人才培养普遍存在的问题,结合我校水产专业人才培养的现状、目标定位与特色发展方向,笔者以教学一线教师的视角,从构建战略联盟长效机制的前提与基础、战略联盟的确定与遴选、人才培养知识体系的构建、专业技能及综合素质培养体系构建等方面,提出自己对“荆楚卓越水产人才”协同培养的一些思考与建议,以期为本校水产专业的学科建设和人才培养提供思路与探讨主题,并通过逐步教学实践探索,进而形成一套专业特色鲜明,能充分适应社会和行业需求的人才培养模式.
语种：
中文
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镧对稀有鮈鲫肝胰脏抗氧化酶活性及MDA含量的影响
作者：
华东;王剑伟;胡兵;刘军
期刊：
水生态学杂志 ,2019年40(2):108-113 ISSN：1674-3075
作者机构：
武汉轻工大学动物科学与营养工程学院;[王剑伟] 中国科学院水生生物研究所;[胡兵] 福建省特种水产配合饲料重点实验室;[华东; 刘军] 武汉轻工大学
关键词：
稀有鮈鲫;镧;超氧化物歧化酶;过氧化氢酶;丙二醛
摘要：
以氯化镧(LaCl_3·7H_2O)为试验毒物,以稀有鮈鲫(Gobiocypris rarus)为试验材料,采用21 d慢性毒性试验(La~(3+)浓度分别为0.00、0.04、0.08、0.16、0.32、0.80 mg/L),通过检测稀有鮈鲫肝胰脏中超氧物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量,研究了镧暴露胁迫对水生动物抗氧化系统的影响,以期从抗氧化生理响应的角度探讨稀土元素镧对水生动物的毒理机理。结果显示,与对照组相比,0.04~0.32 mg/L试验组肝胰脏SOD活性出现一定程度降低(P>0.05),但0.80 mg/L组SOD活性出现一定程度上升(P>0.05);0.04~ 0.32 mg/L试验组肝胰脏CAT活性较对照组出现一定程度降低(P>0.05),但0.80 mg/L组CAT活性出现一定程度上升(P>0.05),且0.80 mg/L组CAT活性显著高于0.04~0.32 mg/L组(P<0.05);0.04~0.16 mg/L试验组肝胰脏MDA含量较对照组出现一定程度降低(P>0.05),0.32 mg/L组MDA含量略微升高(P>0.05), 0.80 mg/L组MDA含量显著高于对照组(P<0.05),也显著高于其它La~(3+)暴露组(P<0.05)。研究表明,一定浓度的La~(3+)暴露胁迫会对环境中水生生物抗氧化系统产生不利影响。
语种：
中文
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水产养殖专业《渔业资源调查》实习的教学设计与探索
作者：
刘军;刘立鹤;余登航;黄峰;李娇;...
期刊：
武汉轻工大学学报 ,2019年38(5):103-107 ISSN：2095-7386
作者机构：
武汉轻工大学 动物科学与营养工程学院,湖北 武汉,430023;[刘军; 刘辉宇; 李娇; 余登航; 黄峰; 刘立鹤] 武汉轻工大学
关键词：
水产养殖专业;渔业资源调查;实践教学
摘要：
根据新形势下应用型人才培养的需求,阐述了《渔业资源调查》实习课程的设立缘由。依据武汉轻工大学水产养殖本科人才培养方案,结合课程教学进程安排,探索、设计了《渔业资源调查》实习课程的实施时间、实施地点、教学内容、实习方式、考核内容与考核办法,探讨了实习期间的教学手段。
语种：
中文
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饲料中添加稀土壳聚糖蝥合盐对鲫鱼肌肉生化组成的影响
作者：
邱逸忱;汪明;刘军
期刊：
饲料工业 ,2018年39(18):25-29 ISSN：1001-991X
作者机构：
武汉轻工大学动物科学与营养工程学院,湖北武汉,430023;[刘军; 汪明; 邱逸忱] 武汉轻工大学
关键词：
稀土壳聚糖螯合盐;鲫鱼;肌肉品质;生化组成
摘要：
通过在日粮中添加不同剂量稀土壳聚糖螯合盐（rare earth-chitosan chelate, RECC）,研究其对鲫鱼（Carassius auratus）肌肉品质的影响。试验分为4个组:空白对照组、0.08%组、0.16%组、0.24%组,各组日粮中稀土壳聚糖螯合盐添加水平分别为0.00%、0.08%、0.16%、0.24%。每组设5个重复,每个重复放养35尾试验鱼。采用生物化学分析和高效液相色谱法分析肌肉中营养组分。结果表明:在同一解冻时间时,各RECC添加组鲫鱼肌肉渗出性损失均较空白对照组有不同程度降低（P>0.05）;各RECC添加组水分、粗脂肪、磷含量与空白对照组较为接近（P>0.05）,粗蛋白质、钙含量均较空白对照组增加（P>0.05）,灰分含量降低（P>0.05）。各RECC添加组鲫鱼肌肉中肌苷含量均高于或显著高于空白对照组,其中0.16%组显著高于空白对照组（P<0.05）;肌苷酸含量也均高于空白对照组（P>0.05）;胆固醇含量则均较空白对照组有不同程度下降（P>0.05）。以上结果表明,稀土壳聚糖螯合盐对鲫鱼肌肉成分不仅无不良影响,而且对其品质、风味有一定增强改善作用。
语种：
中文
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Effects of Acute Copper Exposure on Physiological and Cytological Responses in Liver of GIFT Tilapia (Oreochromis niloticus)
作者：
Yu, Deng-Hang;Chang, Jia-Zhi;Dong, Gui-Fang;Liu, Jun*
期刊：
ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH ,2018年70:1-8 ISSN：0792-156X
通讯作者：
Liu, Jun
作者机构：
[Chang, Jia-Zhi; Dong, Gui-Fang; Liu, Jun; Yu, Deng-Hang] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.;[Dong, Gui-Fang; Liu, Jun; Yu, Deng-Hang] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Hubei, Peoples R China.
通讯机构：
[Liu, Jun] W;[Liu, Jun] F;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.;Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Hubei, Peoples R China.
关键词：
copper toxicity;GIFT tilapia;ultrastructure of liver;lipid metabolism
摘要：
This study was designed to evaluate the physiological and cytological responses in liver of GIFT tilapia (Oreochromis niloticus) to acute copper exposure. Fish were exposed to three Cu treatments at the following concentrations: 0 (control), 1.96 mg/L and 3.92 mg/L, and liver samples were collected after 96h of exposure. Observation of oil red O staining showed that acute copper exposure increased lipid accumulation in liver, which may due to changes of hepatic enzyme activities (malic enzyme, lipoprotein lipase, hepatic lipase, fatty acid synthetase, carnitine palmitoyltransferases l) and lesions of some organelles such as mitochondria and swollen rough endoplasmic reticulum of hepatocytes. This study indicated that acute copper exposure induced hepatic lipid metabolic disturbances and damaged hepatic organelle in GIFT tilapia. © 2018, Israeli Journal of Aquaculture - Bamidgeh. All rights reserved.
语种：
英文
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观赏水族创意性人才培养模式的改革与探索
作者：
刘立鹤;肖航;蒋加鹏;刘军;李娇
期刊：
武汉轻工大学学报 ,2017年36(3):96-100 ISSN：2095-7386
作者机构：
武汉轻工大学动物科学与营养工程学院,武汉,430023;[刘军; 肖航; 李娇; 蒋加鹏; 刘立鹤] 武汉轻工大学
关键词：
观赏水族;创意性人才;培养模式;实践性环节;慕课
摘要：
根据当前国内高校观赏水族科学与技术专业培养目标和学科建设纲要,结合水族行 业发展趋势和市场需求特点,立足于本科应用型创意人才的学科定位及水产养殖学科的现状 与特色,通过增补主干课程架构建设、增加课堂和课后实践性环节要素、引入基于项目的学习 教学、辅以超星慕课和移动学习终端平台建设,延展课堂教学、重塑课程评分体系等方面的改 革与探索,以期建立一套适应学校发展现状,以培养学生学习和实践技能为核心,以教师授课 为主线,以行业发展趋势为导向的观赏水族创意性人才培养模式,从而彰显该专业人才培养特 色,以满足市场对应用型创意性水族人才的需求.
语种：
中文
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Lanthanum exerts acute toxicity and histopathological changes in gill and liver tissue of rare minnow (Gobiocypris rarus)
作者：
Hua, Dong;Wang, Jianwei;Yu, Denghang;Liu, Jun*
期刊：
Ecotoxicology ,2017年26(9):1207-1215 ISSN：0963-9292
通讯作者：
Liu, Jun
作者机构：
[Yu, Denghang; Hua, Dong; Liu, Jun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.;[Wang, Jianwei] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
通讯机构：
[Liu, Jun] W;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
Toxicity;Gobiocypris rarus;La(III);Histopathological;Gill;Liver
摘要：
We evaluated the acute toxicity effects of lanthanum (La(III)) on gill and liver of rare minnow (Gobiocypris rarus). The median lethal concentration of La (III) at 96 h was 1.92 mg L–1. Rare minnow were reared in freshwater and exposed to 0.04, 0.08, 0.16, 0.32 and 0.80 mg L–1 La (III) for 21 d. Gill and liver samples were analyzed by light microscopy. The main histopathological changes induced by La (III) in gills were epithelial lifting, filamentary epithelial proliferation,edema, lamellar fusion, desquamation, and necrosis. Histopathological changes induced by La (III) in the liver included dilation of sinusoids, focal congestion, pyknotic nuclei, karyohexis and karyolysis, vacuolar degeneration, and numerous necrosis areas. Hypsometric analysis indicated significant changes in the measures of gill dimensions (average length, width, area), suggesting metabolic disturbance (gas exchange) upon La (III) exposure. The result showed that La (III) severely affects fish gill and liver. © 2017, Springer Science+Business Media, LLC.
语种：
英文
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Effects of various levels of dietary copper supplementation with copper sulfate and copper hydroxychloride on Pacific white shrimp Litopenaeus vannamei performance and microbial communities
作者：
Zhou, Yangen;Zhang, Dongdong;Peatman, Eric;Rhodes, Melanie A.;Liu, Jun;...
期刊：
Aquaculture ,2017年476:94-105 ISSN：0044-8486
通讯作者：
Davis, D. Allen
作者机构：
[Zhou, Yangen] Ocean Univ China, Minist Educ, Key Lab Mariculture, 5 Yushan Rd, Qingdao 266003, Peoples R China.;[Zhang, Dongdong; Rhodes, Melanie A.; Davis, D. Allen; Peatman, Eric] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, 203 Swingle Hall, Auburn, AL 36849 USA.;[Liu, Jun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, 63 Xuefunanlu, Wuhan 430070, Peoples R China.
通讯机构：
[Davis, D. Allen] A;Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, 203 Swingle Hall, Auburn, AL 36849 USA.
关键词：
Copper hydroxychloride;Copper sulfate;Diet;Litopenaeus vannamei;Microbiome
摘要：
A 42–day trial was conducted to evaluate the performance of juvenile (0.30 g initial weight) Pacific white shrimp Litopenaeus vannamei offered diets containing various levels of copper hydroxychloride (Cu2(OH)3Cl) containing 58.8% copper (Cu) or Cu sulfate (CuSO4) containing 25.5% Cu. The basal diet (360 g/kg protein, 80 g/kg lipid) was primarily composed of soybean meal, fish meal, corn protein isolate and whole wheat and was formulated to be deficient in Cu (approximately 10 mg Cu/kg). The basal diet (B10) was supplemented with 30, 90, 150, and 210 mg Cu/kg from each Cu source. With the exception of 1 treatment (H220, Cu supplemented diet with dietary copper 210 mg/kg from Cu hydroxychloride, n = 4), five replicate groups of 15 shrimp per tank (initial weight 0.30 g) were offered the test diets in slight excess. At the end of the trial, no significant differences were observed in final biomass (53.20–64.40 g), final mean weight (4.34–4.87 g), weight gain (1349–1544%), feed conversion ratio (FCR, 1.61–1.82), survival (81.3–96.0%), and thermal growth coefficient (0.082–0.087). The carapace, hepatopancreas, and whole shrimp Cu were significantly increased with dietary Cu levels from either source. No significant differences in hemolymph Cu contents were observed between the two sources. The Cu concentrations of hepatopancreas and whole shrimp were significantly lower in shrimp fed diets with Cu hydroxychloride than those in shrimp fed diets with Cu sulfate. The Cu contents of final whole body significantly increased with dietary Cu levels from either source and corresponded to a decreased trend in whole body Cu retention as dietary Cu increased regardless of source. Sequencing of intestinal and fecal microbiomes (16S V1–V3) captured distinct microbial communities based on source but not copper treatment group. However, the abundance of several specific family and genera of bacteria did appear to respond to different types and doses of dietary copper. Among these we observed a trend of decreased abundance of particular species of Vibrios, including V. harveyi, V. sinaloensis and V. orientalis. Results of this study confirm that both copper sources are utilized efficaciously by Pacific white shrimp, however, differential deposition patterns indicated the two sources of Cu are metabolized differently. Hence, additional studies to assess the specific metabolic pathways of various Cu sources as well as possible effects of high copper levels on resistance to infection should be undertaken in the future. Statement of relevance In this study, we assessed the efficacy of various levels of dietary copper supplementation with copper sulfate and copper hydroxychloride in Pacific white shrimp. Our finding shows that both copper sources are utilized efficaciously but metabolized differently by shrimp. This manuscript provides valuable information about effects of high copper levels from two copper sources in shrimp. © 2017 Elsevier B.V.
语种：
英文
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Effects of various dietary levels of copper hydroxychloride on growth performance and tissue response in Pacific white shrimp Litopenaeus vannamei fed practical diets
作者：
Zhou, Y.;Rhodes, M. A.;Liu, J.;Davis, D. A.*
期刊：
Aquaculture Nutrition ,2017年23(1):171-180 ISSN：1353-5773
通讯作者：
Davis, D. A.
作者机构：
[Davis, D. A.; Zhou, Y.; Rhodes, M. A.] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, 203 Swingle Hall, Auburn, AL 36849 USA.;[Liu, J.] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan, Peoples R China.
通讯机构：
[Davis, D. A.] A;Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, 203 Swingle Hall, Auburn, AL 36849 USA.
关键词：
bioavailability;copper hydroxychloride;growth performance;Litopenaeus vannamei;tissue response
摘要：
Two trials were conducted to evaluate the performance of Pacific white shrimp Litopenaeus vannamei offered diets containing various copper (Cu) levels from Cu hydroxychloride (Cu2(OH)3Cl) containing 58.81% copper in the clear water recirculating system. In both trials, the basal diet (360 g kg−1 protein, 80 g kg−1 lipid) containing approximately 10 mg Cu kg−1 was primarily comprised of fishmeal, soybean meal, corn protein concentrate and whole wheat. In trial 1, test diets were produced supplementing the basal diet with 5, 10, 20, 40 and 60 mg Cu kg−1 from Cu hydroxychloride. Four replicate groups of 15 shrimp per tank (initial weight 0.28 g) were offered diets in slight excess over 8 weeks. In trial 2, the basal diet was supplemented with 30, 90, 150, 210 and 270 mg Cu kg−1 from Cu hydroxychloride. Seven replicate groups of 15 shrimp per tank (initial weight 0.22 g) were offered feed in slight excess over 7 weeks. At the end of the two growth trials, no significant differences were observed in final biomass, final mean weight, percentage weight gain, feed conversion ratio (FCR) and survival. In trial 1, the Cu concentrations of the carapace, hepatopancreas and whole shrimp linearly increased with increasing dietary Cu supplements. In trial 2, polynomial regression of Cu concentrations of the carapace, hepatopancreas and whole shrimp against analysed dietary Cu content indicated that a plateau was reached at 215 mg analysed Cu kg−1. Results of this study indicate that there was no negative effect of high levels of Cu supplement with regard to growth and survival. Tissue levels generally increased up to around 200 mg Cu kg−1 diet and then decreased, possibly indicating a shift in physiology.
语种：
英文
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Growth performance and whole-body composition of yellow catfish (Pelteobagrus fulvidraco Richardson) under feeding restriction
作者：
Dong, G. F.;Yang, Y. O.* ;Yao, F.;Chen, L.;Yue, D. D.;...
期刊：
Aquaculture Nutrition ,2017年23(1):101-110 ISSN：1353-5773
通讯作者：
Yang, Y. O.
作者机构：
[Liu, L. H.; Huang, F.; Yu, D. H.; Dong, G. F.; Liu, J.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Liu, L. H.; Huang, F.; Yu, D. H.; Dong, G. F.; Liu, J.] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan, Hubei, Peoples R China.;[Yao, F.; Yue, D. D.; Chen, L.; Yang, Y. O.] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.
通讯机构：
[Yang, Y. O.] A;Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.
关键词：
amino acid composition;fatty acid profile;feeding rate restriction;growth performance;Pelteobagrus fulvidraco
摘要：
Fish growth is an important index in aquaculture practice. However, the effect of growth under restricted feeding on quality of fish grown to the same size remains poorly unknown. In this study, yellow catfish were reared by feeding them at three different feeding rates such as low (FR-L group), medium (FR-M group) and high (apparent satiation) ration levels for 82, 58 and 34 days to reach the same size, respectively. After this period, the growth performance, whole-body amino acid composition and fatty acid profile of the fish were evaluated. Feeding restriction led to significant decreases in specific growth rate and feed conversion efficiency. Significant increases in dry matter, protein and energy contents were observed in fish under both restricted feeding treatments. FR-L-treated fish had significantly lower whole-body essential amino acid contents, and significantly higher Ala and non-essential amino acid contents compared to fish fed to satiation. Fish in restricted feeding treatments had significantly higher n-3 PUFA and n-3/n-6 ratio compared to the fish fed to satiation. The present results indicate that from a quality perspective, there are certain advantages in improvement of whole-body composition and enrichment of n-3 PUFA associated with the restricted feeding of yellow catfish at slow growth rate.
语种：
英文
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Effects of Biofloc on Plankton Abundance in the Water
作者：
Nie, Wei;Liu, Wen;Liu, Li-He* ;Yu, Deng-Hang;Liu, Jun;...
期刊：
ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH ,2017年69(1) ISSN：0792-156X
通讯作者：
Liu, Li-He
作者机构：
[Nie, Wei; Liu, Li-He; Liu, Jun; Liu, Wen; Yu, Deng-Hang] Wuhan Polytech Univ, Coll Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.;[Zhou, Zhe] BIOMAX Ecol Engn Ltd, Nanjing 210019, Jiangsu, Peoples R China.
通讯机构：
[Liu, Li-He] W;Wuhan Polytech Univ, Coll Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.
关键词：
Bacillus subtilis;Biofloc;Glucose;Plankton;Water quality indicators
摘要：
This study was conducted to evaluate the effects of biofloc on the species and quantity of plankton in pond water. There were four treatments in the experiment: the control, no supplementation; treatment I, pond water supplemented with glucose; treatment II, pond water supplemented with both glucose and Bacillus subtilis; and treatment III, pond water supplemented with Bacillus subtilis only. Water quality, plankton, and biofloc formation were monitored every five days. Results showed that biofloc formation was enhanced by adding glucose to the pond water. The addition of glucose together with Bacillus subtilis shortened the formation time of mature bioflocs by about five days than the supplementation with glucose only. In ponds supplemented with glucose and those supplemented with glucose + bacillus, the ammonia nitrogen, and nitrite nitrogen content in the water column decreased, and the number of cyanobacteria and green algae were significantly inhibited, while the number of zooplankton such as Rotatoria increased during the experimental period. © 2017, Israeli Journal of Aquaculture - Bamidgeh. All rights reserved.
语种：
英文
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Replacement of fish oil with soybean oil in diets for juvenile Chinese sucker (Myxocyprinus asiaticus): effects on liver lipid peroxidation and biochemical composition
作者：
Yu, Deng-Hang* ;Chang, Jia-Zhi;Dong, Gui-Fang;Liu, Jun
期刊：
Fish Physiology and Biochemistry ,2017年43(5):1413-1420 ISSN：0920-1742
通讯作者：
Yu, Deng-Hang
作者机构：
[Chang, Jia-Zhi; Dong, Gui-Fang; Liu, Jun; Yu, Deng-Hang] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Yu, Deng-Hang] W;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
Chinese sucker;Soybean oil;Lipid peroxidation;Biochemical composition
摘要：
This study was designed to evaluate the effect of the replacement of fish oil (FO) by soybean oil (SO) on growth performance, liver lipid peroxidation, and biochemical composition in juvenile Chinese sucker, Myxocyprinus asiaticus. Fish (13.7±0.2g) in triplicate were fed five experimental diets in which 0% (FO as control), 40% (SO40), 60% (SO60), 80% (SO40), and 100% (SO100) FO were replaced by SO. The body weight gain of fish fed SO40, SO60, or SO80 diet was similar to FO group, but diets that have 100% soybean oil as dietary lipid significantly reduced fish growth (P<0.05). Although the level of SO resulted in increasing crude lipid content of the liver, the level of SO did not significantly alter the hepatosomatic index (HSI). Indicators of peroxidation, such as vitamin E (VE) and thiobarbituric acid-reactive substance (TBARS) contents, were changed as increasing dietary SO. It was shown that the inclusion of SO in the diets increased VE concentrations, but reduced TBARS in the liver and total cholesterol (T-CHO) in the plasma. Linoleic acid (LA) and linolenic acid (LNA) significantly increased in fish liver fed diets that contained SO, but eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and the ratio n-3/n-6 were significantly reduced by the inclusion of dietary SO (P<0.05). Our results indicated that the inclusion of SO increased the hepatic VE content and reduced lipid peroxidation in fish. However, diet containing 100% SO as dietary lipid could reduce growth performance. Thus, we recommended that 40–80% SO can be used as dietary lipid to replace FO for juvenile Chinese sucker. © 2017, Springer Science+Business Media Dordrecht.
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英文
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Dietary supplementation with glutamate precursor alpha-ketoglutarate attenuates lipopolysaccharide-induced liver injury in young pigs
作者：
Wang, Lei;Hou, Yongqing* ;Yi, Dan;Li, Yongtang;Ding, Binying;...
期刊：
Amino Acids ,2015年47(7):1309-1318 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Xiao, Hang; Hou, Yongqing; Liu, Jian; Wang, Lei; Li, Yongtang; Wu, Guoyao; Ding, Binying; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
alpha-Ketoglutarate;Piglets;Lipopolysaccharide;Liver injury
摘要：
Abstract There is growing interest in glutamate as a functional amino acid in nutrition and health. This study was conducted to determine whether glutamate precursor α-ketoglutarate (AKG) could alleviate lipopolysaccharide (LPS)-induced liver injury in young pigs. Twenty-four piglets were randomly assigned to the control, LPS, or LPS + AKG group. Piglets in the control and LPS groups were fed a basal diet, whereas piglets in the NAC group were fed the basal diet supplemented with 1 % AKG. On days 10, 12, 14, and 16 of the trial, piglets in the LPS and LPS + AKG groups received intraperitoneal administration of LPS (80 μg/kg BW), whereas piglets in the control group received the same volume of saline. On day 16 of the trial, blood samples were collected 3 h after LPS or saline injection. Twenty-four hours post-administration of LPS or saline (on day 17 of the trial), piglets were killed to obtain liver for analysis. Dietary AKG supplementation alleviated LPS-induced histomorphological abnormalities and mitigated LPS-induced increases in aspartate aminotransferase (AST) activity and AST/ALT ratio (P < 0.05). Compared with the LPS group, dietary supplementation with AKG decreased plasma glutamate concentration, while increasing hepatic concentrations of glutamate, glutamine, leucine, asparagine, lysine, alanine, serine, threonine, valine, and phenylalanine (P < 0.05). LPS challenge dramatically increased concentrations of malondialdehyde and decreased glutathione peroxidase activity in the liver. Additionally, LPS challenge enhanced concentrations of AMP and total protein, as well as RNA/DNA and total protein/DNA ratios, while decreasing hepatic ADP concentrations. These adverse effects of LPS challenge were ameliorated by AKG supplementation. Collectively, dietary AKG supplementation provides a new means to ameliorate LPS-induced liver injury by increasing anti-oxidative capacity and improving energy metabolism in young pigs. © 2015 Springer-Verlag Wien.
语种：
英文
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Expression of monellin in a food-grade delivery system in Saccharomyces cerevisiae
作者：
Liu, Jun;Yan, Da-zhong* ;Zhao, Sheng-jun
期刊：
Journal of the Science of Food and Agriculture ,2015年95(13):2646-2651 ISSN：0022-5142
通讯作者：
Yan, Da-zhong
作者机构：
[Yan, Da-zhong; Liu, Jun] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Zhao, Sheng-jun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Yan, Da-zhong] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
关键词：
fungal DNA;ribosome DNA;sweetening agent;vegetable protein;Africa;chemistry;food handling;fruit;gene expression;genetic transformation;genetics;human;Menispermaceae;metabolism;plant gene;Saccharomyces cerevisiae;Africa, Western;DNA, Fungal;DNA, Ribosomal;Food Technology;Fruit;Gene Expression;Genes, Plant;Humans;Menispermaceae;Plant Proteins;Saccharomyces cerevisiae;Sweetening Agents;Transformation, Genetic
摘要：
BACKGROUND: Genetically modified (GM) foods have caused much controversy. Construction of a food-grade delivery system is a desirable technique with presumptive impact on industrial applications from the perspective of bio-safety. The aim of this study was to construct a food-grade delivery system for Saccharomyces cerevisiae and to study the expression of monellin from the berries of the West African forest plant Dioscoreophyllum cumminsii in this system. RESULTS: A food-grade system for S. cerevisiae was constructed based on ribosomal DNA (rDNA)-mediated homologous recombination to enable high-copy-number integration of the expression cassette inserted into the rDNA locus. A copper resistance gene (CUP1) was used as the selection marker for yeast transformation. Because variants of transformants containing different copy numbers at the CUP1 locus can be readily selected after growth in the presence of elevated copper levels, we suggest that this system would prove useful in the generation of tandemly iterated gene clusters. Using this food-grade system, a single-chain monellin gene was heterologously expressed. The yield of monellin reached a maximum of 675 mg L-1. CONCLUSION: This system harbors exclusively S. cerevisiae DNA with no antibiotic resistance genes, and it should therefore be appropriate for safe use in the food industry. Monellin was shown to be expressed in this food-grade delivery system. To our knowledge, this is the first report so far on expression of monellin in a food-grade expression system in S. cerevisiae. © 2015 Society of Chemical Industry.
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英文
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生物絮团的研究进展
作者：
聂伟;刘立鹤;刘军;黄峰;王哲;...
期刊：
江西水产科技 ,2014年(4):43-48 ISSN：1006-3188
作者机构：
武汉轻工大学动物科学与营养工程学院;[王哲; 周哲] 标优美生态工程股份有限公司;[刘军; 聂伟; 黄峰; 刘立鹤] 武汉轻工大学
关键词：
生物絮团;絮凝机理;影响因素;应用问题
摘要：
生物絮团是养殖水体中以好氧微生物为主体的有机体和无机物，经生物絮凝形成的团聚物，由细菌、浮游动植物、有机碎屑和一些无机物质相互絮凝组成。通过对生物絮团研究的发展过程、絮团的结构特征、絮凝机理、影响絮团的形成因素以及生物絮团技术在水产养殖应用中存在的问题进行了综述，为生物絮团技术在水产领域中的进一步研究和应用具有一定的指导意义。
语种：
中文
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草鱼配合饲料添加酵母培养物对草鱼生长性能、肝肠功能的影响
作者：
刘立鹤;张恒;聂伟;陈进;张志勇;...
期刊：
武汉轻工大学学报 ,2014年33(1):34-39 ISSN：2095-7386
作者机构：
武汉轻工大学动物科学与营养工程学院;湖北省淡水水产健康养殖协同创新中心;[范伟] 武汉爱维信生物科技有限公司;[刘军; 聂伟; 张恒; 张志勇; 陈进; 黄峰; 刘立鹤] 武汉轻工大学
关键词：
草鱼;酵母培养物;生长性能;肝胰腺;组织切片
摘要：
试验在两种不同营养水平的饲料配方中分别添加0 mg/kg,50 mg/kg,100 mg/kg的酵母培养物益宁易SCP,制成6种试验饲料饲喂均重20.39±2.19g草鱼6周,评估了饲料中不同益宁易添加量对草鱼生长性能和肝、肠功能的影响.结果表明:在普通饲料中添加50 mg/kg的益宁易SCP可以促进草鱼的生长,保护草鱼的肝脏不受损害,并能够显著促进草鱼肠道的发育,增加其绒毛高度;而在降低营养标准的低价饲料中,添加量要需增加到100 mg/kg,效果才会更明显.
语种：
中文
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饲喂枯草芽孢对草鱼粪便、养殖水体中芽孢数量和水质的影响
作者：
贺国龙;张恒;刘立鹤;刘军;黄峰;...
期刊：
淡水渔业 ,2012年42(2):35-39 ISSN：1000-6907
作者机构：
[贺国龙; 张恒; 刘立鹤] 武汉工业学院动物科学与营养工程学院;[刘军; 黄峰] 湖北省饲料工程技术研究中心;[董爱华] 广州雅琪生物科技有限公司
关键词：
草鱼(Ctenopharyngodon idellus);枯草(Bacillus subtilis)芽孢;粪便;水质
摘要：
分别在饲料中添加不同含量的枯草(Bacillus subtilis)芽孢(A组实测芽孢数量为2.6×10~7 cfu/g,B组为4.4×10~6 cfu/g,空白组为0),饲喂静水水族箱中100 g左右的草鱼(Ctenopharyngodon idellus)10 d,测定饲喂枯草芽孢后草鱼粪便、养殖水体中芽孢数量和水质的变化。结果表明:(1)9 d内,水体中芽孢数量A组和B组在前6 d时不断增加,在6 d时达到稳定,其中A组和B组稳定时的含量分别为2.22×10~6 cfu/g和3.98×10~5 cfu/g。(2)10 d内,草鱼粪便干物质中的芽孢数量与饲料中的枯草芽孢含量相比,A组和B组粪便中的枯草芽孢损失率分别为89.76%~90.71%和83.83%~86.84%。(3)草鱼饲喂枯草芽孢后,A组和B组粪便中排出的枯草芽孢对水体中亚硝酸盐和氨氮有降低的趋势,但各组之间差异不显著(P>0.05),对化学需氧量(COD)没有影响。
语种：
中文
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不同加工工艺及保存方式对饲料中芽孢数量的影响
作者：
贺国龙;刘立鹤;张恒;刘军;谭斌;...
期刊：
武汉轻工大学学报 ,2012年31(2):15-19 ISSN：2095-7386
作者机构：
武汉工业学院动物科学与营养工程学院;[刘军; 张恒] 湖北省饲料工程技术研究中心;[周晓辉; 谭斌] 安琪酵母股份有限公司;[贺国龙; 刘立鹤] 武汉工业学院
关键词：
商品饲料;饲料加工工艺;保存方式;枯草芽孢
摘要：
采用A和B两种商品饲料加工工艺,分别在饲料配方中添加9.10×107cfu/g和1.05×107cfu/g的枯草芽孢,依次记为A1、A2、B1、B2,系统研究了不同加工工艺和芽孢添加量对饲料中芽孢数量的影响;并进一步研究了B1、B2在密闭和敞开两种不同保存方式下,饲料中芽孢数量的变化规律。研究结果表明:（1）在A和B生产工艺条件下,随着加工工段的先后,饲料芽孢的数量呈现递减趋势,A1和A2的成品芽孢存活率分别为37.47%和41.90%,B1和B2的成品存活率分别为28.35%和41.90%。（2）以低剂量饲料芽孢数量为横坐标,高剂量饲料芽孢数量为纵坐标,则在各工段中高剂量组与低剂量组呈现较好的线性回归关系,这表明枯草芽孢的存活率不受添加剂量的影响。（3）在100 d的保存期内,B1和B2饲料中芽孢数量与第1 d相比,芽孢的数量没有显著变化。
语种：
中文
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凡纳滨对虾饲料中菜籽粕替代鱼粉适宜比例的研究
作者：
张恒;刘立鹤;贺国龙;刘军;黄峰
期刊：
水产科学 ,2011年30(10):591-596 ISSN：1003-1111
作者机构：
武汉工业学院动物科学与营养工程学院;[刘军] 湖北省饲料工程技术研究中心;武汉工业学院动物营养与饲料科学湖北省重点实验室;[贺国龙; 张恒; 黄峰; 刘立鹤] 武汉工业学院
关键词：
凡纳滨对虾;菜籽粕;鱼粉;蛋白源
摘要：
以饲料中添加36％鱼粉作为对照组,用菜籽粕分别替代饲料配方中10％～50％的鱼粉,配成5种等氮等能试验饲料,饲喂凡纳滨对虾,研究不同替代比例对凡纳滨对虾生长性能和虾体氨基酸组成的影响.试验结果表明,饲养56d后,随着菜籽粕替代鱼粉比例的增加,对虾的质量增加率下降,但仅50％替代比例试验组的质量增加率为1542.74％,显著低于对照组(2066.07％,P＜0.05)；质量增加率(y)和菜籽粕实际添加量(x)的回归方程为y=-0.8674x2+12.917x +2031.7(r2=0.9081).通过拐点法计算得出的菜籽粕最适添加量为7.37％(换算成替代比例为12.70％),在99％的置信水平下,3.17％～22.48％的替代是完全可行的.菜籽粕替代鱼粉饲喂凡纳滨对虾,对其存活率、饵料系数、虾体氨基酸总量和鲜味氨基酸含量影响不大,当替代比例达到50％时,其存活率、虾体蛋氨酸和精氨酸含量才显著低于对照组,饵料系数显著高于对照组.综合凡纳滨对虾质量增加率、存活率、饵料系数、虾体氨基酸含量等各项指标,凡纳滨对虾饲料中用菜籽粕替代22.48％鱼粉完全可行.
语种：
中文
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Alpha-ketoglutarate and intestinal function
作者：
Hou, Yongqing* ;Wang, Lei;Ding, Binying;Liu, Yulan;Zhu, Huiling;...
期刊：
FRONTIERS IN BIOSCIENCE-LANDMARK ,2011年16(1):1186-1196 ISSN：2768-6701
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Yulan; Kang, Ping; Hou, Yongqing; Liu, Jian; Wang, Lei; Li, Yongtang; Ding, Binying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Peoples R China.;China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Alpha-ketoglutarate;Gut function;Intestine;Lipopolysaccharide;Piglets;Review
摘要：
Alpha-ketoglutarate (AKG) is an intermediate of the Krebs cycle which bridges amino acid metabolism with glucose oxidation in animals. Of particular interest is the conversion of AKG into glutamate by mitochondrial glutamate dehydrogenase in the gastrointestinal tract where glutamate has multiple physiological functions (including regulation of cell function, neurotransmission, and gastric emptying). Additionally, AKG stimulates the initiation of catabolism of branched-chain amino acids (BCAA) via BCAA transaminase in enterocytes. Oxidation of AKG also provides large amounts of ATP and modulates cellular redox state in the small intestine. Translating the basic research into practice, results of recent studies indicate that dietary supplementation with AKG alleviates oxidative stress and injury in intestinal mucosal cells, while improving intestinal mucosal integrity and absorption of nutrients in endotoxin-challenged pigs. The beneficial effects of AKG are associated with increased activation of the mTOR signaling pathway and net protein synthesis. Thus, AKG is a novel and promising supplement in diets to improve intestinal health in animals and possibly humans.
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英文
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